[S](mmol/L) Sin inhibidor Con inhibidor Diagrama de LINEAWEAVER

1.25 1.72 0.98 Sin inhibidor Linear (Sin inh

1.67 2.04 1.17 Con inhibidor Linear (Con in

1/V [(mmol/L)min^-1]
2.5 2.63 1.47 1.2
5 3.33 1.96 1
f(x) = 0.858162966866622 x + 0.336927757065883
10 4.17 2.38 0.8
0.6
f(x) = 0.48431276187116 x + 0.195090246656494
0.4
1/[S] 1/V sin 1/V con
0.8 0.581395348837 1.020408163265 0.2
1/[S] (mmol/L)
0.59880239521 0.490196078431 0.854700854701 0
0 0.1 0.2 0.3 0.4 0.5
0.4 0.380228136882 0.680272108844
0.2 0.3003003003 0.510204081633
0.1 0.239808153477 0.420168067227

b). Sin Con a).

Vmáx 5.125576627371 2.968239833779 Es una inhibición no competitiva, porque KM se mantiene y
KM 2.482316760636 2.547343425349 reduce
agrama de LINEAWEAVER-BURK
Sin inhibidor Linear (Sin inhibidor)
Con inhibidor Linear (Con inhibidor)

162966866622 x + 0.336927757065883

31276187116 x + 0.195090246656494

1/[S] (mmol/L)
0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9

etitiva, porque KM se mantiene y Vmáx se

[S](mmol/L) Sin inhibidor Inhibidor B 3 mM Inhibidor B 5mM
1.25 1.72 1.25 1.01
1.67 2.04 1.54 1.26
2.5 2.63 2 1.72
5 3.33 2.86 2.56
10 4.17 3.7 3.49

1/[S] 1/V Sin 1/V con B a 3mM 1/V con B a 5mM

0.8 0.58139535 0.8 0.990099009901
0.5988024 0.49019608 0.64935064935065 0.7936507936508
0.4 0.38022814 0.5 0.5813953488372
0.2 0.3003003 0.34965034965035 0.390625
0.1 0.23980815 0.27027027027027 0.2865329512894

b). Sin Con B a 3mM Con B a 5 mM a).

Vmáx 5.12557663 5.07872016251905 5.3734551316497 inhibición competitiva, KM s
KM 2.48231676 3.83443372270188 5.4067705534659 mantiene constante y la velocid
aumenta
 LINEAWEAVER-BURK
1.2

1
f(x) = 1.00624910965659 x + 0.186077012430725
1/V[(mmol/L) min^-1

0.8
f(x) = 0.754987560008577 x + 0.196940313994366
0.6 sin
f(x) = 0.48431276187116 x + 0.195090246656494
0.4 a 3 mM
a 5mM
0.2

0 [I]
0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9
3 Mm
1/[S](mmol/L) 5 mM

1/V Sin Linear (1/V Sin) 1/V con B a 3mM

Linear (1/V con B a 3mM) 1/V con B a 5mM Linear (1/V con B a 5mM)

KM con I=KM sin I (1+[I]/Ki)

c). a 3mM
inhibición competitiva, KM se KM CON-KM SIN= KM SIN* [I] / Ki
mantiene constante y la velocidad 1.35211696206631 7.446950281907 /Ki
aumenta Despejando:
Ki= 5.507622854258
 KM con KM sin
2.48231676
3.83443372
5.40677055

0.8 0.9

5mM)

in I (1+[I]/Ki)
d). a 5mM
KM CON-KM SIN= KM SIN* [I] / Ki
2.92445379283031 12.41158380318 /Ki
Despejando
Ki= 4.244069040724