CÁLCULO DE ENZIMA Y GRÁFICA DE MICHAEL MENTEN

Velocidad
mM/seg/mg
Glucosa mM enzima 1/m 1/Vo
0.05 30 20 0.03333333
0.1 43 10 0.02325581 Chart Title
0.15 52 6.66666667 0.01923077 0.035
0.2 57 5 0.01754386 f(x) = 0.00100921927206192 x + 0.013038056551442
0.3 58 3.33333333 0.01724138 0.03

0.025
GRÁFICA MICHAELIS-MENTEN
0.02

0.015

0.01
𝑦=𝑚𝑥+𝑏 0.005

0
1) y= 2 4 6 8 10 12 14 16
vmax=
km=
2) 1/vmax=
3) km/vmax=
 Chart Title
𝑦=𝑚𝑥+𝑏

00921927206192 x + 0.0130380565514427
Vmax 0.013
km 0.01

1/vmx 76.9230769

km/vmax 0.7623077

8 10 12 14 16 18 20 22
CALCULO DE INHIBIDORES

Sin Inhibidor Inhibidor A Inhibidor B

v0 v0 v0 3000000
[S] M M/s M/s M/s
2500000 f(x) = 1
0.0005 0.00000125 0.00000058 0.00000038 R² = 0.
0.001 0.000002 0.00000104 0.000000603 2000000
0.0025 0.00000313 0.000002 0.000001 1500000 f(x) = 7
0.005 0.00000385 0.00000278 0.00000125 R² = 0.
0.01 0.00000455 0.00000357 0.00000143 1000000
f(x) = 3
500000 R² = 0.
Enzima pura Inhibidor A Inhibidor B
0
1/[s] 1/v0 1/v0 1/v0 0
1/M s/M s/M s/M
2000 800000 1724137.931 2631578.947
1000 500000 961538.4615 1658374.793
400 319488.8179 500000 100000
200 259740.2597 359712.2302 800000 km
100 219780.2198 280112.0448 699300.6991 VMAX

GRÁFICA DE LINEWEAVER BURK

1) Calcular el valor de km y Vmax de la enzima

2) Determine el tipo de inhibición causada por A y B y determinar el valor de KI

NOMBRE: ADAMARIS CARRIÓN

 Chart Title
3000000

2500000 f(x) = 1022.41685770007 x + 601262.4131764

R² = 0.999392860018852
2000000

1500000 f(x) = 760.071796158343 x + 202647.004364165

R² = 0.999947197973117
1000000
f(x) = 302.77294538748 x + 195749.879895635
500000 R² = 0.999728217816058
0
0 500 1000 1500 2000 2500

Series1 Linear (Series1)

Series3 Linear (Series3)
Series5 Linear (Series5)
SIN INH CON INH
KM= Km(1+I/kI) 0.00154672 0.00170042
0,001700423=0,001546718(1+0,00000038/KI) 5.10856E-06 1.66317E-06
0,001700423=0,001546718(1)+0,001546718(0,00000038/KI)
0,001700423-0,001546718=0,001546718(0,00000038)/KI

0,00015371= /Ki
5,8775x10-10
KI=5,8775x10-10 /0,00015371
KI= -64998,801

0,001546718/-64998,801= -2.37961005e-8 //

OMBRE: ADAMARIS CARRIÓN PARDO