Vías metabólicas en E.

coli

1.1. Compuestos del ciclo de Krebs de E. coli

 Citrato
 Isocitrato
 αCetoGlutarato
 SuccinilCoA
 Succinato
 Fumarato
 Malato
 Oxalacetato
 Enzimas del ciclo de Krebs de E. coli

Citrato
sintasa Aconitasa

Malato Isocitrato
deshidrogenasa deshidrogenasa

αCetoGlutarato
deshidrogenasa
Fumarato
hidratasa

Succinil CoA
Succinato sintetasa
deshidrogenasa

N° ID ENZYME GENE REACTION EQUATION CHEBI IUPAC

b1276 (acnA)
01 ACONTa Acotinase cit_c ⇌ acon_C_c + h2o_c
b0118 (acnB)
Isocitrate icit_x + nadp_x ⇌ akg_x +
02 ICDHym YDL066W (IDP1)
dehydrogenase co2_x + nadph_x
α-KetoGlutarate nad_c + r2hglut_c ⇌
03 ARHGDx b2913 (serA)
dehydrogenase akg_c + h_c + nadh_c
Succinil CoA b0729 (sucD) atp_c + coa_c + succ_c ⇌
04 SUCOAS
synthetase b0728 (sucC) adp_c + pi_c + succoa_c
b0724 (sdhB)
Succinate b0721 (sdhC) fad_c + succ_c → fadh2_c
05 SUCD1
dehydrogenase b0723 (sdhA) + fum_c
b0722 (sdhD)
b4152 (frdC)
Fumarate b4154 (frdA) fum_c + q8h2_c ⇌ q8_c +
06 FRD7
hydratase b4153 (frdB) succ_c
b4151 (frdD)
Malate mal__L_c + nad_c ⇌ h_c
07 MDH b3236 (mdh)
dehydrogenase + nadh_c + oaa_c
accoa_c + h2o_c + oaa_c
08 CS Citrate synthase b0720 (gltA)
→ cit_c + coa_c + h_c
 1.2. Enzimas de la Vía pentosa fosfato de E. coli

Glucosa 6-Fosfato
Glucosa 6-fosfato
deshidrogenasa
deshidrogenasa

Fosfogluconato
deshidrogenasa

Ribulosa 5-fosfato Ribulosa 5-fosfato

isomerasa epimerasa

Ribulosa 5-fosfato Transcetolasa Transaldolasa

epimerasa

Transaldolasa Transaldolasa

Transaldolasa Transcetolasa

PHASE N° ID ENZYME GENE REACTION EQUATION CHEBI IUPAC

G6PDH2 Glucose 6-phosphate g6p_c + nadp_c ⇌

01 b1852 (zwf)
r dehydrogenase 6pgl_c + h_c + nadph_c
Fase
Oxidativa 6pgc_c + nadp_c →
Phosphogluconate
02 GND b2029 (gnd) co2_c + nadph_c +
dehydrogenase
ru5p__D_c
Ribulose 5-
b2914 (rpiA)
03 RPI phosphate r5p_c ⇌ ru5p__D_c
b4090 (rpiB)
isomerase
Ribulose 5- b3386 (rpe)
ru5p__D_c ⇌
Fase No 04 RPE phosphate b4301 (sgcE
xu5p__D_c
oxidativa epimerase )
b2465 (tktB) r5p_c + xu5p__D_c ⇌
05 TKT1 Transketolase
b2935 (tktA) g3p_c + s7p_c
b0008 (talB) g3p_c + s7p_c ⇌ e4p_c
06 TALA Transaldolase
b2464 (talA) + f6p_c