Nombre: Diego Tobar

Curso: Cuarto Biotecnología A

Docente :Ing. Cecilia Carpio
 X Y
Dobles Inversos Gliceroquinasa -Lineweaver Burk
10 0.02
0.035
20 0.025
y = 0.0004x + 0.0175
30.303 0.03003 0.03

40 0.03003 0.025

0.02

Vmax=57.14 mg/min 0.015

Km=Vmax*0.0035 0.01
Km=0.1999 mM
0.005

0
0 5 10 15 20 25 30 35 40 45

Eadie-Hofter Gliceroquinasa Eadie-Hofter

60
X=Vo Y=Vo/s
500 50 50
800 40
1009.09 33.3 40
1332 33.3 Km=0.0206 mM y = -0.0206x + 57.92
30
Vmas=57.92
mg/min
20

10

0
0 200 400 600 800 1000 1200 1400
 Hanes -
Wolf

X=S Y=S/Vo
Gliceroquinasa Hanes-Wolf
0.1 0.002 0.0025

0.05 0.00125
0.002 y = 0.016x + 0.0004
0.033 0.0009909 Vmax=62.5 mg/min
0.025 0.0007507 Km=Vmax*0.0004
0.0015
Km=-0.025 mM

0.001

0.0005

0
0 0.02 0.04 0.06 0.08 0.1 0.12
Trabajo Asistido
 X Y
Dobles Inversos Transaminasa
3.33 5.882 7
2 3.703
6
1 2.325 y = 1.4581x + 0.9195

0.5 1.538 5

0.33 1.369 Vmax=1.078 mg/min 4

0.25 1.282 Km=Vmax*1.45
3
0.2 1.265 Km=1.563 uM
0.1 1.234 2

0
0 0.5 1 1.5 2 2.5 3 3.5

Eadie-Hofter

Transaminasa
X=Vo Y=Vo/s
0.566 0.17 1
0.9
0.54 0.27
0.8
0.43 0.43 Km=1.37 uM
0.7
0.325 0.65 Vmas=1.015 mg/min
0.6
0.243 0.73
0.5
0.195 0.78 y = -1.3757x + 1.0152
0.4
0.158 0.79
0.3
0.081 0.81 0.2
0.1
0
0 0.1 0.2 0.3 0.4 0.5 0.6
 Hanes -
Wolf

X=S Y=S/Vo

0.3 1.764 Transaminasa

0.5 1.851 14
1 2.325 Vmax=0.915 mg/min 12
y = 1.0921x + 1.094
2 3.076 Km=Vmax*1.0921 10
3 4.109 Km=1.001 uM
8
4 5.128
6
5 6.329
10 12.345 4
2
0
0 2 4 6 8 10 12