OBJETIVO 

Calcular la concentración de cada uno de los componentes de una mezcla 

MATERIAL Y REACTIVOS. 
 Espectrofotómetro Globe Modelo CS200PC 
 Tubos de ensayo 
 Gradilla 
 Pipeta 
 Agua destilada 
 Solución A (un estándar de c conocida) 
 Solución B (un estándar de c conocida) 
 Mezcla de A y B 
 
Cálculos
510 nm 630 nm
Rojo 0.798 0.007
Azul 0.037 1.055
Jamaica GV 1.600 0.299
Jamaica Light 1.625 0.226

 Concentración en mezclas
A x = A B+ A 2 x

1. GV
3.55 g
=0.0142 M
250 mL
2. Clight
3.58 g
=0.01432 M
250 mL
3. Azul
0.0527 g −4
=5.27 x 10 M
100 mL

4. Rojo
0.0637 g −4
=6.37 x 10 M
100 mL

mg
C Azul=0.52
mL
mg
C Rojo =0.637
mL
  Absortividades
A
a=
bC
 AZUL
A 0.037 mL
a A 510 = ⟹ a A 510 = =0.1423
bC
(
( 1 cm ) 0.26
mg
mL ) cm mg

A 1.055 mL
a A 630 = ⟹ a A 630 = =4.0576
bC
(
(1 cm ) 0.26
mg
mL ) cmmg

 ROJO
A 0.798 mL
a A 510 = ⟹ a A 510 = =2.5054
bC
(
( 1 cm ) 0.26
mg
mL ) cmmg

A 0.007 mL
a A 630 = ⟹ a A 630 = =0.0219
bC
(
(1 cm ) 0.26
mg
mL ) cm mg

 Absorbancias
AT 510= A R 510 + A A 510 AT 510= A R 510 + A A 510

A=abc

( cmmg
AT 510= 2.505
mL
) ( 1 cm ) (0.3185 mLmg
)+(0.1423 cmmLmg )(1 cm) ¿
=(0.0219
cmmg ) ( mL )
mL mg mL
AT 630 ( 1 cm ) 0.3185 +(4.0576 )(1 cm)¿
cmmg

 JAMAICA GV
A510 =1.600
A630 =0.299

AT 510=a R 510 · b· C R + a A 510 · b · C A

AT 630 =a R 630 · b ·C R + a A 630 · b · C A

(
1.600= 2.505
mL
cmmg ) ( mL
( C R ) + 0.1423 cmmg ( C A ) … ( 1 ) )
 (
0.299= 0.021
mL
cmmg
( ) (
C R )+ 04.057
mL
cm mg )
( C A ) …( 2)

[ 1.600=( 2.505 ) ( C R ) +( 0.1423 ) ( C A ) ] (−4.057)

[ 0.299=( 0.021 ) ( C R ) +( 04.057 ) ( C A ) ] ( 0.1423 )
−6.4912=−10.1627C R −0.5773
−3
0.0425=2.98 x 10 C R +0.5773
−6.4487=−10.1597
−6.4487
=C R
−10.1597
mg
C R =0.634 7
mL
Sustituimos C R en 1
1.600= ( 2.505 )( 0.6347 )+ ( 0.1423 ) ( C A )
1.600= ( 2.505 )( 0.6347 )+ 0.1423C A
1.600=1.5899+ 0.1423C A
1.600−1.5899=0.142 3C A
0.0101=0.1423C A
0.0101
=C A
0.1423
mg
C A=0.07097
mL

 JAMAICA CLIGHT
AT 510=a R 510 · b· C R + a A 510 · b · C A

AT 630 =a R 630 · b ·C R + a A 630 · b · C A

( cmmg
1.625= 2.505
mL
) (C )+(0.142 cmmg
R
mL
) (C ) … ( 1) A

0.226=( 0.021 ) C ) +( 04.057

cm mg )
mL mL
cm mg
( R (C ) … ( 2) A

[ 1.625=( 2.505 ) ( C R ) +( 0.1423 ) ( C A ) ] (−4.057)

 [ 0.226=( 0.021 ) ( C R ) +( 04.057 ) ( C A ) ] ( 0.1423 )

−6.5926=−10.1627 C R−0.5773
−3
0.03215=2.98 x 10 C R +0.5773
−6.5604=−10.1597
−6.5604
=C R
−10.1597
mg
C R =0.6 457
mL
Sustituimos C R en 1
1.625= ( 2.505 )( 0.6457 )+ ( 0.1423 ) ( C A )
1.625= ( 2.505 )( 0.6457 )+ 0.1423C A
1.625=1.6174 +0.1423 C A
1.625−1.6174=0.142 3 C A
−3
7.6 x 10 =0.1423 C A
7.6 x 10−3
=C A
0.1423
mg
C A=0.05340
mL