Práctica Acetilcolinesterasa - Aa2022

1.
1.4
f(x) = − 1253.16302521008 x² + 105.509142857143 x + 0.425546192226891
1.2
0.8
0.6
0.4
f(x) = − 56.2689075630276 x² + 12.6741428571429 x + 0.206432451418067

0.2
0
0:00:00 0:02:52 0:05:45 0:08:38 0:11:31 0:14:24 0:17:16
ACT_ENZ Polynomial (ACT_ENZ) 50uMDNPZ Polynomial (50uMDNPZ)

Método 6
ORDEN FILA A
1 BUFFER C pH 8
2 MeOH:H20/EXTRACTO
3 DTNB (3 mM) TRIS 50mM pH8
4 ATCh /SUSTRATO (15mM) AGUA
5 DONEPEZILO
PRE-INCUBACIÓN (412nm-cinetica) 25°c
AChE (1 U/mL) (15 ul + 1985 BUFFER C-TRIS -50mM pH8)
Cinética 412 nm 60 min (c/1mins) 25 °C
A
B
C
D
E
F
G
H
OBSERVACIONES:
Concentraciones iniciales inhibibdor Donepez

CTRL_DONP:4 = 5uM
DONP:3 = 10 uM
DONP:2 = 50 uM
DONP:1 = 100 uM
Software Version 2.09.1
Experiment File Path: C:\Users\Public\Documents\Experiments\Practica_G8.xpt

Protocol File Path: C:\Users\Public\Documents\Protocols\AChE\AChE_4_PRACTICAS_19042022.prt
Plate Number Plate 1
Date 5/24/2022
Time 17:46:17
Reader Type: Epoch 2
Reader Serial Number:Unknown
Reading Type Reader
Procedure Details
Plate Type Nunclon 96 flat bottom
Eject plate on completion
Set Temperature Setpoint 25°C, Gradient 0 °C
Preheat before moving to next step
Start Kinetic Runtime 0:03:00 (HH:MM:SS), Interval 0:01:00, 4 Reads
Shake Orbital: Continuous
Frequency: 282 cpm (3 mm)
Read Absorbance Endpoint
Full Plate
Wavelengths: 412
Read Speed: Normal, Delay: 100 msec, Measurements/Data Point: 8
End Kinetic
Plate Out/In AÑADIR AChE
Shake Orbital: 1:00 (MM:SS)
Full Plate
Wavelengths: 412
End Kinetic
Layout
Read 1:412
Time
0:00:15
0:01:15
0:02:15
0:03:15
Read 2:412
Time
0:00:15
0:01:15
0:02:15
0:03:15
0:04:15
0:05:15
0:06:15
0:07:15
0:08:15
0:09:15
0:10:15
0:11:15
0:12:15
0:13:15
0:14:15
0:15:15
Blank Read 2:412
Time
0:00:15
0:01:15
0:02:15
0:03:15
0:04:15
0:05:15
0:06:15
0:07:15
0:08:15
0:09:15
0:10:15
0:11:15
0:12:15
0:13:15
0:14:15
0:15:15
Results
B
B
H
CTRL_DON
BLK ACT_ENZ P
80 40 58
20 metanol 20 metanol -
100 100 100
- 20 20
- - 2
3 mins
- 20 20
15min 15min 15min
PARA LA PRUEBA peso inicial uL peso final (mg/mL)

Mínimo Buffer C 10000
ATCh + PBS (4.3mg en 1mL=15mM 4.3 1000 4.30
DTNB + BUFFER C (1.19 mg en 1 5.95 5000 1.19
AChE + BUFFER C
PARA REACTIVOS BUFFER C 15000 PREPARAR 10 mL

Soluciones Molaridad InicMolaridad final 10mL
MgCl2.6H20 (0,08M
molaridad soluciones
preparadas) 0.08 0.02 2.5
NaCl (0,4 M molaridad
soluciones preparadas) 0.4 0.1 2.5
Tris (200mM molaridad
Ajustar a pH8 con HCl 2M (140uL) y ajustar volumen a

10mL
Agua ultrapura 2.5
1 2 3 4 5
BLK BLK BLK ACT_ENZ ACT_ENZ
CTRL_DON CTRL_DONP: CTRL_DON
CTRL_DONP:4 CTRL_DONP:4
P:4 3 P:3
Concentraciones iniciales inhibibdor Donepezilo:

CTRL_DONP:4 = 5uM
DONP:3 = 10 uM
DONP:2 = 50 uM
DONP:1 = 100 uM
tica_G8.xpt
ChE_4_PRACTICAS_19042022.prt
urements/Data Point: 8
1 2 3 4 5

15 15
P:4 3 P:3
5 5 5 10 10
T° Read 1:412 G1 G2 G3 G4
25.2 0.057 0.053 0.077 0.208
25.2 0.057 0.053 0.077 0.211
25.2 0.057 0.053 0.077 0.214
25.2 0.057 0.053 0.077 0.219
T° Read 2:412 G1 G2 G3 G4
25.4 0.057 0.053 0.077 0.441
25.4 0.057 0.053 0.077 0.51
25.4 0.057 0.053 0.077 0.588
25.5 0.057 0.053 0.077 0.663
25.5 0.057 0.053 0.077 0.729
25.5 0.057 0.053 0.077 0.8
25.6 0.057 0.053 0.077 0.867
25.6 0.057 0.053 0.077 0.922
25.7 0.057 0.053 0.077 0.984
25.7 0.057 0.053 0.077 1.049
25.7 0.057 0.053 0.077 1.11
25.8 0.057 0.053 0.077 1.171
25.8 0.057 0.053 0.077 1.231
25.9 0.057 0.053 0.077 1.287
25.9 0.057 0.053 0.077 1.347
25.9 0.057 0.053 0.077 1.409
G1 G2 G3 G4 G5
0.222 0.266
0.291 0.317
0.369 0.381
0.444 0.451
0.51 0.512
0.581 0.578
0.648 0.643
0.703 0.701
0.765 0.772
0.83 0.831
0.891 0.888
0.952 0.954
1.012 1.013
1.068 1.076
1.128 1.142
1.19 1.2
1 2 3 4 5
-0.1 0.1 0.067 77.9 71.033
1 1 1 1 1
0:02:45 0:02:45 0:05:45 0:01:45 0:07:45
0:02:55 0:02:35 0:06:45 0:00:21 0:01:08
34.767 36.9 31.567 21.667 23.667
1 1 1 1 1
0:00:45 0:02:45 0:03:45 0:04:45 0:00:45
0:00:15 0:00:23 0:00:57 0:00:38 0:00:15
6 7 8 9 10
ACT_ENZ ACT_ENZ ACT_ENZ ACT_ENZ
CTRL_DON CTRL_DON CTRL_DON
P:3 P:2 P:1
6 7 8 9 10

15 15 15 15
P:3 P:2 P:1
10 50 50 50 100
G5 G6 G7 G8 G9
0.209 0.198 0.201 0.208 0.198
0.217 0.203 0.207 0.213 0.203
0.223 0.207 0.213 0.218 0.209
0.227 0.211 0.216 0.224 0.214
G5 G6 G7 G8 G9
0.485 0.437 0.432 0.464 0.434
0.536 0.517 0.498 0.545 0.5
0.6 0.583 0.596 0.618 0.585
0.67 0.649 0.678 0.687 0.663
0.731 0.713 0.749 0.759 0.75
0.797 0.775 0.835 0.826 0.824
0.862 0.83 0.926 0.896 0.909
0.92 0.877 0.987 0.968 0.973
0.991 0.931 1.059 1.033 1.033
1.05 0.981 1.129 1.109 1.115
1.107 1.04 1.184 1.165 1.18
1.173 1.089 1.25 1.229 1.24
1.232 1.147 1.31 1.298 1.301
1.295 1.21 1.381 1.364 1.363
1.361 1.263 1.446 1.426 1.426
1.419 1.323 1.508 1.49 1.485
G6 G7 G8 G9 H1
0.218 0.213 0.245 0.215 0.099
0.298 0.279 0.326 0.281 0.133
0.364 0.377 0.399 0.366 0.165
0.43 0.459 0.468 0.444 0.197
0.494 0.53 0.54 0.531 0.227
0.556 0.616 0.607 0.605 0.254
0.611 0.707 0.677 0.69 0.283
0.658 0.768 0.749 0.754 0.309
0.712 0.84 0.814 0.814 0.338
0.762 0.91 0.89 0.896 0.37
0.821 0.965 0.946 0.961 0.396
0.87 1.031 1.01 1.021 0.426
0.928 1.091 1.079 1.082 0.451
0.991 1.162 1.145 1.144 0.479
1.044 1.227 1.207 1.207 0.508
1.104 1.289 1.271 1.266 0.534
6 7 8 9 10
80.767 98.4 81.567 86.867
1 1 1 1
0:00:45 0:01:45 0:00:45 0:03:45
0:00:15 0:00:35 0:00:15 0:00:37
17.733 9.167 10.667 11.1 11.133
1 1 1 1 1
0:07:45 0:00:45 0:04:45 0:01:45 0:09:45
0:01:20 0:00:15 0:01:04 0:00:17 0:02:29
11 12
CTRL_DON
CTRL_DONP:1
P:1
11 12
Well ID
Conc/Dil
Well ID
Conc/Dil
Well ID
Conc/Dil
Well ID
Conc/Dil
Well ID
Conc/Dil
Well ID
Conc/Dil
Well ID
Conc/Dil
CTRL_DON
CTRL_DONP:1 Well ID
P:1
100 100 Conc/Dil
H1 H2 H3 H4 H5 H6
0.2 0.166 0.212 0.207 0.204 0.199
0.204 0.169 0.216 0.213 0.202 0.203
0.209 0.173 0.22 0.218 0.207 0.207
0.213 0.177 0.225 0.223 0.212 0.213
H1 H2 H3 H4 H5 H6
0.318 0.287 0.33 0.291 0.291 0.278
0.352 0.323 0.355 0.312 0.314 0.295
0.384 0.356 0.375 0.332 0.335 0.31
0.416 0.393 0.402 0.351 0.355 0.325
0.446 0.426 0.434 0.369 0.375 0.339
0.473 0.458 0.458 0.391 0.396 0.352
0.502 0.486 0.488 0.407 0.417 0.368
0.528 0.518 0.514 0.426 0.439 0.383
0.557 0.549 0.538 0.444 0.458 0.401
0.589 0.58 0.558 0.463 0.478 0.418
0.615 0.608 0.587 0.478 0.497 0.434
0.645 0.639 0.602 0.495 0.516 0.45
0.67 0.665 0.626 0.511 0.536 0.464
0.698 0.698 0.651 0.528 0.555 0.479
0.727 0.726 0.669 0.545 0.574 0.494
0.753 0.754 0.697 0.562 0.594 0.51
H2 H3 H4 H5 H6 H7
0.068 0.111 0.072 0.072 0.059 -0.011
0.104 0.136 0.093 0.095 0.076 -0.001
0.137 0.156 0.113 0.116 0.091 0.007
0.174 0.183 0.132 0.136 0.106 0.016
0.207 0.215 0.15 0.156 0.12 0.024
0.239 0.239 0.172 0.177 0.133 0.033
0.267 0.269 0.188 0.198 0.149 0.042
0.299 0.295 0.207 0.22 0.164 0.05
0.33 0.319 0.225 0.239 0.182 0.058
0.361 0.339 0.244 0.259 0.199 0.066
0.389 0.368 0.259 0.278 0.215 0.075
0.42 0.383 0.276 0.297 0.231 0.083
0.446 0.407 0.292 0.317 0.245 0.091
0.479 0.432 0.309 0.336 0.26 0.099
0.507 0.45 0.326 0.355 0.275 0.107
0.535 0.478 0.343 0.375 0.291 0.116
11 12
Max V [Blank Read 2:412]
R-Squared [Blank Read 2:412]
t at Max V [Blank Read 2:412]
Lagtime [Blank Read 2:412]

8.3 8.3 Max V [Blank Read 2:412]
1 1 R-Squared [Blank Read 2:412]
0:02:45 0:06:45 t at Max V [Blank Read 2:412]
0:00:22 0:00:49 Lagtime [Blank Read 2:412]

4,000
3,500
3,000
2,500
OD
2,000
1,500
1,500
1,000
0,500
0,000
0:00:00
H7 H8 H9 H10 H11 H12

0.166 0.204 0.193 0.18 0.197 0.2
0.171 0.208 0.198 0.185 0.202 0.204
0.176 0.214 0.202 0.19 0.207 0.209
0.181 0.219 0.208 0.195 0.212 0.214
H7 H8 H9 H10 H11 H12

0.208 0.249 0.251 0.239 0.26 0.247
0.218 0.255 0.261 0.248 0.268 0.255
0.226 0.264 0.272 0.257 0.276 0.262
0.235 0.273 0.282 0.266 0.284 0.27
0.243 0.283 0.292 0.274 0.292 0.277
0.252 0.293 0.301 0.282 0.299 0.284
0.261 0.303 0.311 0.29 0.307 0.292
0.269 0.312 0.321 0.298 0.315 0.3
0.277 0.321 0.33 0.306 0.323 0.306
0.285 0.331 0.339 0.315 0.33 0.314
0.294 0.34 0.348 0.326 0.338 0.32
0.302 0.349 0.357 0.335 0.345 0.328
0.31 0.357 0.367 0.345 0.353 0.335
0.318 0.367 0.376 0.352 0.36 0.342
0.326 0.376 0.385 0.36 0.367 0.349
0.335 0.385 0.395 0.368 0.375 0.356
H8 H9 H10 H11 H12

0.03 0.032 0.02 0.041 0.028
0.036 0.042 0.029 0.049 0.036
0.045 0.053 0.038 0.057 0.043
0.054 0.063 0.047 0.065 0.051
0.064 0.073 0.055 0.073 0.058
0.074 0.082 0.063 0.08 0.065
0.084 0.092 0.071 0.088 0.073
0.093 0.102 0.079 0.096 0.081
0.102 0.111 0.087 0.104 0.087
0.112 0.12 0.096 0.111 0.095
0.121 0.129 0.107 0.119 0.101
0.13 0.138 0.116 0.126 0.109
0.138 0.148 0.126 0.134 0.116
0.148 0.157 0.133 0.141 0.123
0.157 0.166 0.141 0.148 0.13
0.166 0.176 0.149 0.156 0.137
4,000
3,500
3,000
2,500
2,000
1,500
1,500
1,000
0,500
0,000
0:00:00 0:10:00 0:20:00 0:30:00 0:40:00 0:5
Time
D10-Read 2:412
D11-Read 2:412
D12-Read 2:412
F4-Read 2:412
F7-Read 2:412
F10-Read 2:412
F4-Read 2:412
F7-Read 2:412
F10-Read 2:412
0:40:00 0:50:00 1:00:00 1:10:00

e
10-Read 2:412
11-Read 2:412
12-Read 2:412
4-Read 2:412
7-Read 2:412
10-Read 2:412
4-Read 2:412
7-Read 2:412
10-Read 2:412
GRUPO 8 GRUPO 7
Time G4 A4
0:00:15 0.441 0.71
0:01:15 0.51 0.867
0:02:15 0.588 1.084
0:03:15 0.663 1.337
0:04:15 0.729 1.485
0:05:15 0.8 1.748
0:06:15 0.867 1.951
0:07:15 0.922 2.217
0:08:15 0.984 2.632
0:09:15 1.049 2.891
0:10:15 1.11 3.015
0:11:15 1.171 3.169
0:12:15 1.231 3.325
0:13:15 1.287 3.458
0:14:15 1.347 3.565
0:15:15 1.409 3.662
Chart Title
4
3.5
3
2.5
2
1.5
1
0.5
0
0:00:00 0:02:52 0:05:45 0:08:38 0:11:31 0:14:24 0:17:16
G4 A4
GRUPO 8
Time G4 G5 G6
0:00:15 0.441 0.485 0.437
0:01:15 0.51 0.536 0.517
0:02:15 0.588 0.6 0.583
0:03:15 0.663 0.67 0.649
0:04:15 0.729 0.731 0.713
0:05:15 0.8 0.797 0.775
0:06:15 0.867 0.862 0.83
0:07:15 0.922 0.92 0.877
0:08:15 0.984 0.991 0.931
0:09:15 1.049 1.05 0.981
0:10:15 1.11 1.107 1.04
0:11:15 1.171 1.173 1.089
0:12:15 1.231 1.232 1.147
0:13:15 1.287 1.295 1.21
0:14:15 1.347 1.361 1.263
0:15:15 1.409 1.419 1.323
Grupo 7
PROMEDIO desvest A6 A7 A8 PROMEDIO desvest
0.45 0.03 0.582 0.623 0.704 0.64 0.06
0.52 0.01 0.727 0.791 0.946 0.82 0.11
0.59 0.01 0.89 0.99 1.116 1.00 0.11
0.66 0.01 0.998 1.182 1.324 1.17 0.16
0.72 0.01 1.136 1.406 1.525 1.36 0.20
0.79 0.01 1.29 1.636 1.738 1.55 0.23
0.85 0.02 1.426 1.821 1.949 1.73 0.27
0.91 0.03 1.681 2.05 2.111 1.95 0.23
0.97 0.03 1.852 2.258 2.308 2.14 0.25
1.03 0.04 2.034 2.428 2.471 2.31 0.24
1.09 0.04 2.218 2.652 2.615 2.50 0.24
1.14 0.05 2.398 2.782 2.777 2.65 0.22
1.20 0.05 2.536 2.984 2.97 2.83 0.25
1.26 0.05 2.642 3.1 3.103 2.95 0.27
1.32 0.05 2.701 3.242 3.225 3.06 0.31
1.38 0.05 2.862 3.361 3.386 3.20 0.30
Método 6,
ORDEN FILA A
1 BUFFER C pH 8
2 MeOH:H20/EXTRACTO
5 DONEPEZILO
A
B
C
D
E
F
G
H
OBSERVACIONES:

CTRL_DONP:4 = 5uM
DONP:3 = 10 uM
DONP:2 = 50 uM
DONP:1 = 100 uM

Procedure Details
Full Plate
Wavelengths: 412
End Kinetic
Full Plate
Wavelengths: 412
End Kinetic
Layout
Read 1:412
Time
0:00:15
0:01:15
0:02:15
0:03:15
Read 2:412
Time
0:00:15
0:01:15
0:02:15
0:03:15
0:04:15
0:05:15
0:06:15
0:07:15
0:08:15
0:09:15
0:10:15
0:11:15
0:12:15
0:13:15
0:14:15
0:15:15
Blank Read 2:412
Time
0:00:15
0:01:15
0:02:15
0:03:15
0:04:15
0:05:15
0:06:15
0:07:15
0:08:15
0:09:15
0:10:15
0:11:15
0:12:15
0:13:15
0:14:15
0:15:15
Results
B
B
H
CTRL_DON
BLK ACT_ENZ P
80 40 58
20 metanol 20 metanol -
100 100 100
- 20 20
- - 2
3 mins
- 20 20
15min 15min 15min

AChE + BUFFER C

MgCl2.6H20 (0,08M
molaridad soluciones
preparadas) 0.08 0.02 2.5

10mL
Agua ultrapura 2.5
1 2 3 4 5
P:4 3 P:3

CTRL_DONP:4 = 5uM
DONP:3 = 10 uM
DONP:2 = 50 uM
DONP:1 = 100 uM
tica_G7.xpt
1 2 3 4 5
15 15
P:4 3 P:3
5 5 5 10 10
T° Read 1:412 A1 A2 A3 A4
25 0.212 0.216 0.172 0.253
25 0.214 0.215 0.174 0.261
25 0.216 0.216 0.175 0.264
25 0.217 0.218 0.176 0.271
T° Read 2:412 A1 A2 A3 A4
25.1 0.224 0.225 0.181 0.71
25.1 0.226 0.227 0.182 0.867
25.1 0.228 0.228 0.184 1.084
25.1 0.229 0.23 0.185 1.337
25.1 0.231 0.232 0.187 1.485
25.2 0.232 0.233 0.188 1.748
25.2 0.234 0.235 0.189 1.951
25.2 0.236 0.236 0.191 2.217
25.2 0.237 0.238 0.193 2.632
25.2 0.239 0.24 0.194 2.891
25.2 0.24 0.241 0.195 3.015
25.2 0.242 0.243 0.197 3.169
25.2 0.244 0.244 0.198 3.325
25.3 0.245 0.246 0.199 3.458
25.3 0.247 0.248 0.2 3.565
25.3 0.249 0.249 0.202 3.662
A1 A2 A3 A4 A5
0.014 0.015 -0.029 0.499 0.48
0.014 0.015 -0.029 0.656 0.651
0.015 0.015 -0.029 0.871 0.875
0.014 0.015 -0.029 1.122 1.061
0.014 0.015 -0.029 1.268 1.26
0.014 0.015 -0.03 1.53 1.495
0.015 0.015 -0.03 1.731 1.741
0.015 0.015 -0.03 1.996 1.993
0.014 0.015 -0.03 2.41 2.255
0.015 0.016 -0.031 2.667 2.409
0.015 0.016 -0.031 2.79 2.589
0.015 0.016 -0.031 2.942 2.726
0.015 0.016 -0.031 3.096 2.919
0.015 0.016 -0.031 3.228 3.01
0.015 0.016 -0.031 3.333 3.151
0.015 0.016 -0.032 3.429 3.296
1 2 3 4 5
0.633 0.633 -0.733 414.1 262.4
1 1 1 1 1
0:01:45 0:02:45 0:08:45 0:07:45 0:07:45
0:00:50 0:02:53 0:06:53 0:03:38 0:01:29
163.233 145.367 120.833 82.733 86.833
1 1 1 1 1
0:01:45 0:00:45 0:02:45 0:05:45 0:01:45
0:00:31 0:00:15 0:00:45 0:00:52 0:00:27
6 7 8 9 10
P:3 P:2 P:1
6 7 8 9 10
15 15 15 15
P:3 P:2 P:1
10 50 50 50 100
A5 A6 A7 A8 A9
0.275 0.257 0.267 0.238 0.25
0.281 0.269 0.275 0.241 0.252
0.287 0.277 0.283 0.248 0.255
0.293 0.283 0.291 0.256 0.262
A5 A6 A7 A8 A9
0.69 0.582 0.623 0.704 0.672
0.863 0.727 0.791 0.946 0.874
1.089 0.89 0.99 1.116 1.067
1.276 0.998 1.182 1.324 1.199
1.477 1.136 1.406 1.525 1.414
1.712 1.29 1.636 1.738 1.601
1.961 1.426 1.821 1.949 1.805
2.214 1.681 2.05 2.111 1.941
2.478 1.852 2.258 2.308 2.066
2.633 2.034 2.428 2.471 2.198
2.814 2.218 2.652 2.615 2.311
2.954 2.398 2.782 2.777 2.495
3.148 2.536 2.984 2.97 2.624
3.24 2.642 3.1 3.103 2.749
3.382 2.701 3.242 3.225 2.887
3.529 2.862 3.361 3.386 3.012
A6 A7 A8 A9 B1
0.372 0.413 0.494 0.462 0.518
0.516 0.579 0.735 0.663 0.637
0.676 0.776 0.902 0.854 0.8
0.783 0.967 1.109 0.984 0.9
0.919 1.189 1.308 1.198 1.034
1.072 1.419 1.521 1.383 1.128
1.207 1.601 1.73 1.586 1.204
1.46 1.829 1.89 1.72 1.314
1.629 2.035 2.085 1.843 1.391
1.81 2.204 2.247 1.974 1.487
1.993 2.427 2.39 2.086 1.572
2.171 2.555 2.549 2.268 1.68
2.308 2.756 2.741 2.395 1.761
2.412 2.869 2.873 2.519 1.838
2.47 3.011 2.994 2.655 1.943
2.629 3.128 3.152 2.779 2.055
6 7 8 9 10
253.3 229.133 240.367 213.2
1 1 1 1
0:06:45 0:04:45 0:00:45 0:03:45
0:02:57 0:00:52 0:00:15 0:00:48
83.867 30.867 22.9 30.767 19.767
1 1 1 1 1
0:00:45 0:08:45 0:07:45 0:08:45 0:09:45
0:00:15 0:01:43 0:01:19 0:02:08 0:01:46
11 12
CTRL_DON
CTRL_DONP:1
P:1
11 12
Well ID
Conc/Dil
CTRL_DON
CTRL_DONP:1 Well ID
P:1
100 100 Conc/Dil
Well ID
Conc/Dil
Well ID
Conc/Dil
Well ID
Conc/Dil
Well ID
Conc/Dil
Well ID
Conc/Dil
Well ID
Conc/Dil
B1 B2 B3 B4 B5 B6
0.276 0.268 0.271 0.263 0.253 0.272
0.282 0.266 0.277 0.261 0.257 0.276
0.29 0.276 0.279 0.265 0.259 0.282
0.297 0.286 0.283 0.269 0.265 0.286
B1 B2 B3 B4 B5 B6
0.728 0.677 0.694 0.522 0.508 0.548
0.848 0.824 0.772 0.591 0.579 0.633
1.014 0.936 0.879 0.67 0.668 0.71
1.115 1.022 1.002 0.749 0.736 0.785
1.25 1.102 1.108 0.823 0.814 0.858
1.346 1.23 1.201 0.893 0.893 0.913
1.424 1.299 1.284 0.978 0.967 0.993
1.535 1.4 1.385 1.041 1.014 1.055
1.613 1.477 1.468 1.106 1.081 1.104
1.711 1.533 1.557 1.162 1.147 1.171
1.798 1.631 1.642 1.202 1.202 1.239
1.907 1.723 1.723 1.267 1.276 1.303
1.989 1.816 1.812 1.348 1.322 1.372
2.068 1.902 1.892 1.394 1.385 1.436
2.175 1.984 1.97 1.459 1.445 1.494
2.288 2.072 2.063 1.532 1.507 1.562
B2 B3 B4 B5 B6 B7
0.467 0.484 0.312 0.298 0.338 0.162
0.612 0.561 0.379 0.367 0.422 0.187
0.722 0.666 0.456 0.454 0.496 0.21
0.808 0.787 0.534 0.521 0.57 0.238
0.886 0.892 0.607 0.597 0.641 0.262
1.012 0.983 0.675 0.676 0.695 0.285
1.079 1.065 0.758 0.747 0.774 0.308
1.179 1.163 0.82 0.793 0.834 0.337
1.255 1.246 0.884 0.858 0.882 0.364
1.309 1.332 0.938 0.923 0.947 0.395
1.405 1.417 0.977 0.977 1.013 0.421
1.496 1.496 1.04 1.049 1.075 0.442
1.587 1.584 1.12 1.093 1.144 0.461
1.672 1.661 1.164 1.155 1.206 0.48
1.752 1.738 1.227 1.213 1.262 0.499
1.838 1.83 1.299 1.274 1.329 0.522
11 12
15.2 18.633 Max V [Blank Read 2:412]


4,000
3,500
3,000
2,500
D
2,000
2,500
OD
2,000
1,500
1,000
0,500
0,000
0:00:00
B7 B8 B9 B10 B11 B12

0.267 0.242 0.265 0.254 0.25 0.252
0.259 0.25 0.265 0.262 0.258 0.258
0.263 0.253 0.268 0.267 0.262 0.266
0.27 0.254 0.271 0.273 0.266 0.273
B7 B8 B9 B10 B11 B12

0.372 0.336 0.374 0.343 0.324 0.349
0.398 0.356 0.399 0.36 0.339 0.365
0.424 0.379 0.426 0.377 0.354 0.383
0.453 0.4 0.45 0.394 0.368 0.403
0.478 0.421 0.473 0.411 0.382 0.421
0.503 0.443 0.498 0.43 0.398 0.441
0.527 0.464 0.52 0.45 0.414 0.459
0.558 0.483 0.545 0.468 0.431 0.478
0.586 0.507 0.575 0.486 0.447 0.495
0.619 0.528 0.607 0.505 0.463 0.514
0.646 0.545 0.636 0.526 0.478 0.532
0.67 0.565 0.663 0.544 0.495 0.549
0.69 0.586 0.688 0.559 0.51 0.568
0.71 0.602 0.71 0.578 0.526 0.586
0.731 0.617 0.734 0.593 0.542 0.604
0.755 0.639 0.759 0.609 0.556 0.621
B8 B9 B10 B11 B12

0.126 0.164 0.133 0.114 0.139
0.145 0.187 0.148 0.127 0.154
0.165 0.212 0.163 0.14 0.169
0.185 0.235 0.179 0.153 0.188
0.204 0.257 0.195 0.165 0.205
0.225 0.28 0.212 0.18 0.223
0.245 0.301 0.23 0.195 0.24
0.262 0.324 0.247 0.21 0.257
0.285 0.352 0.263 0.225 0.273
0.304 0.383 0.281 0.239 0.29
0.32 0.411 0.3 0.253 0.306
0.337 0.435 0.316 0.268 0.322
0.357 0.46 0.33 0.281 0.339
0.372 0.48 0.348 0.296 0.355
0.386 0.502 0.361 0.31 0.372
0.406 0.526 0.375 0.323 0.388
4,000
3,500
3,000
2,500
2,000
2,500
2,000
1,500
1,000
0,500
0,000
0:00:00 0:10:00 0:20:00 0:30:00 0:40:00 0:5
Time
D10-Read 2:412
D11-Read 2:412
D10-Read 2:412
D11-Read 2:412
D12-Read 2:412
F4-Read 2:412
F7-Read 2:412
F10-Read 2:412
0:40:00 0:50:00 1:00:00 1:10:00

e
10-Read 2:412
11-Read 2:412
10-Read 2:412
11-Read 2:412
12-Read 2:412
4-Read 2:412
7-Read 2:412
10-Read 2:412
Método 6,
ORDEN FILA A
1 BUFFER C pH 8
2 MeOH:H20/EXTRACTO
5 DONEPEZILO
A
B
C
D
E
F
G
H
OBSERVACIONES:

CTRL_DONP:4 = 5uM
DONP:3 = 10 uM
DONP:2 = 50 uM
DONP:1 = 100 uM

Date 5/17/2022
Time 17:48:40
Reader Serial Number: 15051119
Reading Type Reader
Procedure Details
Full Plate
Wavelengths: 412
End Kinetic
Full Plate
Wavelengths: 412
End Kinetic
Layout
Read 1:412
Time
0:00:15
0:01:15
0:02:15
0:03:15
Read 2:412
Time
0:00:15
0:01:15
0:02:15
0:03:15
0:04:15
0:05:15
0:06:15
0:07:15
0:08:15
0:09:15
0:10:15
0:11:15
0:12:15
0:13:15
0:14:15
0:15:15
Blank Read 2:412
Time
0:00:15
0:01:15
0:02:15
0:03:15
0:04:15
0:05:15
0:06:15
0:07:15
0:08:15
0:09:15
0:10:15
0:11:15
0:12:15
0:13:15
0:14:15
0:15:15
Results
C
C
H
CTRL_DON
BLK ACT_ENZ P
80 40 58
20 metanol /buffer 20 metanol -
100 100 100
- 20 20
- - 2
3 mins
- 20 20
15min 15min 15min

AChE + BUFFER C

MgCl2.6H20 (0,08M molaridad

10mL
Agua ultrapura 2.5
1 2 3 4 5
P:4 3 P:3
CTRL_DONP:4 = 5uM
DONP:3 = 10 uM
DONP:2 = 50 uM
DONP:1 = 100 uM
tica_G6.xpt
1 2 3 4 5
15 15
P:4 3 P:3
5 5 5 10 10
T° Read 1:412 A1 A2 A3 A4
25.1 0.238 0.243 0.242 0.241
25.1 0.238 0.245 0.243 0.245
25 0.242 0.249 0.248 0.252
25.1 0.245 0.25 0.248 0.257
T° Read 2:412 A1 A2 A3 A4
25 0.202 0.209 0.208 0.811
25 0.203 0.21 0.216 1.015
25 0.205 0.211 0.212 1.213
25 0.207 0.213 0.213 1.413
25 0.21 0.215 0.221 1.597
25 0.212 0.216 0.221 1.806
25 0.216 0.218 0.22 2.011
25.1 0.217 0.219 0.221 2.217
25 0.218 0.221 0.223 2.412
25.1 0.22 0.222 0.23 2.615
25 0.224 0.224 0.227 2.783
25 0.225 0.225 0.231 2.962
25 0.227 0.226 0.233 3.136
25 0.229 0.228 0.234 3.281
25 0.231 0.23 0.235 3.415
25 0.234 0.233 0.24 3.525
A1 A2 A3 A4 A5
-0.004 0.003 0.002 0.605 0.529
-0.006 0 0.006 0.805 0.675
-0.004 0.001 0.003 1.004 0.88
-0.004 0.002 0.002 1.202 1.062
-0.005 0 0.006 1.382 1.181
-0.004 0 0.004 1.59 1.433
-0.002 0 0.002 1.793 1.627
-0.002 0 0.002 1.998 1.846
-0.002 0 0.002 2.191 2.076
-0.004 -0.002 0.006 2.391 2.284
-0.001 -0.001 0.002 2.558 2.491
-0.002 -0.002 0.004 2.735 2.663
-0.002 -0.002 0.004 2.907 2.835
-0.001 -0.002 0.004 3.051 2.988
-0.001 -0.002 0.003 3.183 3.128
-0.002 -0.003 0.005 3.289 3.246
1 2 3 4 5
3 -2.367 4.333 207.6 252
1 1 1 1 1
0:09:45 0:00:45 0:00:45 0:04:45 0:04:45
0:09:07 0:00:15 0:00:15 0:00:31 0:01:40
100.067 72.7 102.8 79.233 239.833
1 1 1 1 1
0:10:45 0:05:45 0:09:45 0:07:45 0:07:45
0:01:54 0:00:58 0:02:56 0:01:02 0:01:26
6 7 8 9 10
P:3 P:2 P:1
6 7 8 9 10
15 15 15 15
P:3 P:2 P:1
10 50 50 50 100
A5 A6 A7 A8 A9
0.228 0.251 0.253 0.231 0.248
0.233 0.257 0.259 0.237 0.256
0.239 0.26 0.262 0.244 0.262
0.245 0.271 0.269 0.25 0.269
A5 A6 A7 A8 A9
0.735 0.851 0.834 0.671 0.421
0.885 1.018 1.049 0.834 0.491
1.09 1.128 1.246 1.006 0.573
1.273 1.279 1.456 1.173 0.647
1.396 1.467 1.683 1.329 0.744
1.65 1.632 1.901 1.551 0.851
1.845 1.801 2.125 1.773 0.984
2.065 2.049 2.338 1.992 1.094
2.297 2.221 2.543 2.201 1.188
2.509 2.417 2.738 2.396 1.284
2.716 2.733 2.922 2.573 1.367
2.889 2.821 3.096 2.757 1.457
3.063 2.98 3.245 2.929 1.541
3.219 3.126 3.386 3.083 1.625
3.36 3.256 3.511 3.229 1.71
3.481 3.404 3.606 3.361 1.794
A6 A7 A8 A9 B1
0.645 0.627 0.465 0.215 0.248
0.808 0.84 0.624 0.281 0.331
0.919 1.037 0.797 0.364 0.412
1.068 1.246 0.962 0.436 0.497
1.252 1.468 1.114 0.529 0.57
1.415 1.685 1.335 0.635 0.649
1.583 1.907 1.556 0.766 0.727
1.83 2.119 1.773 0.875 0.807
2 2.323 1.98 0.968 0.894
2.192 2.514 2.172 1.06 0.99
2.508 2.698 2.348 1.142 1.084
2.594 2.869 2.53 1.231 1.184
2.752 3.016 2.7 1.313 1.283
2.896 3.155 2.852 1.395 1.349
3.024 3.279 2.997 1.479 1.419
3.169 3.37 3.126 1.559 1.513
6 7 8 9 10
315.4 222.1 221 131
1 1 1 1
0:09:45 0:03:45 0:04:45 0:05:45
0:04:21 0:00:28 0:01:19 0:02:03
45 30.333 26.6 38.733 28.9
1 1 1 1 1
0:09:45 0:13:45 0:04:45 0:13:45 0:09:45
0:01:17 0:02:55 0:01:20 0:02:53 0:03:45
11 12
CTRL_DON
CTRL_DONP:1
P:1
11 12
Well ID
Conc/Dil
CTRL_DON
CTRL_DONP:1 Well ID
P:1
100 100 Conc/Dil
Well ID
Conc/Dil
Well ID
Conc/Dil
Well ID
Conc/Dil
Well ID
Conc/Dil
Well ID
Conc/Dil
Well ID
Conc/Dil
B1 B2 B3 B4 B5 B6
0.26 0.231 0.271 0.244 0.239 0.274
0.265 0.237 0.276 0.249 0.244 0.281
0.271 0.243 0.282 0.255 0.251 0.284
0.277 0.249 0.287 0.262 0.256 0.29
B1 B2 B3 B4 B5 B6
0.455 0.368 0.492 0.411 0.69 0.37
0.541 0.431 0.57 0.482 0.9 0.417
0.621 0.494 0.646 0.559 1.124 0.458
0.708 0.555 0.716 0.634 1.323 0.503
0.785 0.621 0.791 0.704 1.502 0.547
0.865 0.69 0.861 0.777 1.722 0.589
0.944 0.764 0.935 0.849 1.892 0.626
1.026 0.838 1.012 0.916 2.097 0.666
1.114 0.912 1.084 0.997 2.338 0.709
1.214 0.986 1.158 1.071 2.551 0.746
1.309 1.051 1.262 1.142 2.714 0.792
1.411 1.119 1.325 1.223 2.914 0.82
1.512 1.182 1.398 1.297 3.07 0.862
1.58 1.242 1.489 1.368 3.222 0.897
1.651 1.306 1.556 1.438 3.359 0.941
1.748 1.368 1.65 1.508 3.471 0.975
B2 B3 B4 B5 B6 B7
0.162 0.286 0.205 0.484 0.163 0.08
0.221 0.36 0.273 0.69 0.207 0.102
0.285 0.436 0.35 0.915 0.249 0.129
0.344 0.505 0.423 1.112 0.292 0.153
0.406 0.576 0.489 1.287 0.332 0.176
0.473 0.644 0.561 1.505 0.373 0.198
0.546 0.718 0.631 1.675 0.408 0.22
0.619 0.793 0.697 1.878 0.447 0.241
0.691 0.863 0.777 2.117 0.488 0.266
0.762 0.934 0.847 2.327 0.522 0.288
0.826 1.037 0.917 2.489 0.567 0.312
0.893 1.099 0.996 2.687 0.593 0.339
0.953 1.169 1.068 2.841 0.633 0.364
1.011 1.259 1.138 2.992 0.667 0.393
1.074 1.324 1.207 3.127 0.709 0.424
1.132 1.415 1.272 3.236 0.739 0.451
11 12
17.467 18.067 Max V [Blank Read 2:412]


4,000
3,500
3,000
2,500
OD
2,000
1,500
1,500
1,000
0,500
0,000
0:00:00
B7 B8 B9 B10 B11 B12

0.239 0.239 0.274 0.237 0.233 0.268
0.244 0.242 0.281 0.24 0.239 0.276
0.249 0.249 0.287 0.246 0.245 0.282
0.255 0.254 0.294 0.251 0.251 0.287
B7 B8 B9 B10 B11 B12

0.286 0.272 0.342 0.27 0.253 0.295
0.312 0.292 0.373 0.291 0.27 0.312
0.339 0.314 0.405 0.312 0.287 0.329
0.364 0.337 0.437 0.331 0.303 0.348
0.391 0.358 0.469 0.349 0.318 0.365
0.414 0.386 0.503 0.369 0.334 0.383
0.438 0.406 0.536 0.389 0.349 0.402
0.46 0.427 0.568 0.408 0.364 0.42
0.486 0.454 0.603 0.426 0.381 0.438
0.512 0.477 0.635 0.447 0.395 0.455
0.537 0.498 0.666 0.476 0.411 0.47
0.566 0.523 0.702 0.499 0.426 0.486
0.593 0.544 0.738 0.519 0.441 0.502
0.624 0.567 0.768 0.546 0.456 0.518
0.655 0.591 0.808 0.569 0.471 0.534
0.686 0.608 0.847 0.6 0.486 0.549
B8 B9 B10 B11 B12

0.065 0.136 0.064 0.047 0.089
0.082 0.163 0.082 0.06 0.102
0.105 0.195 0.102 0.078 0.12
0.126 0.227 0.12 0.092 0.137
0.143 0.254 0.134 0.103 0.15
0.17 0.287 0.153 0.118 0.167
0.188 0.318 0.171 0.131 0.184
0.208 0.349 0.189 0.145 0.201
0.233 0.383 0.206 0.16 0.217
0.253 0.411 0.223 0.171 0.231
0.273 0.441 0.252 0.187 0.245
0.296 0.475 0.273 0.2 0.259
0.315 0.509 0.291 0.212 0.274
0.337 0.538 0.315 0.226 0.288
0.359 0.576 0.338 0.239 0.302
0.373 0.611 0.364 0.25 0.314
4,000
3,500
3,000
2,500
2,000
1,500
1,500
1,000
0,500
0,000
0:00:00 0:10:00 0:20:00 0:30:00 0:40:00 0:5
Time
D10-Read 2:412
D11-Read 2:412
D12-Read 2:412
F4-Read 2:412
F7-Read 2:412
F10-Read 2:412
F4-Read 2:412
F7-Read 2:412
F10-Read 2:412
0:40:00 0:50:00 1:00:00 1:10:00

e
10-Read 2:412
11-Read 2:412
12-Read 2:412
4-Read 2:412
7-Read 2:412
10-Read 2:412
4-Read 2:412
7-Read 2:412
10-Read 2:412
Método 6,
ORDEN FILA A
1 BUFFER C pH 8
2 MeOH:H20/EXTRACTO
5 DONEPEZILO
A
B
C
D
E
F
G
H
OBSERVACIONES:

CTRL_DONP:4 = 5uM
DONP:3 = 10 uM
DONP:2 = 50 uM
DONP:1 = 100 uM

Date 5/10/2022
Time 18:25:16
Reading Type Reader
Procedure Details
Full Plate
Wavelengths: 412
End Kinetic
Full Plate
Wavelengths: 412
End Kinetic
Layout
Read 1:412
Time
0:00:15
0:01:15
0:02:15
0:03:15
Read 2:412
Time
0:00:15
0:01:15
0:02:15
0:03:15
0:04:15
0:05:15
0:06:15
0:07:15
0:08:15
0:09:15
0:10:15
0:11:15
0:12:15
0:13:15
0:14:15
0:15:15
Blank Read 2:412
Time
0:00:15
0:01:15
0:02:15
0:03:15
0:04:15
0:05:15
0:06:15
0:07:15
0:08:15
0:09:15
0:10:15
0:11:15
0:12:15
0:13:15
0:14:15
0:15:15
Results
A
A
H
CTRL_DON
BLK ACT_ENZ P
80 40 58
100 100 100
- 20 20
- - 2
3 mins
- 20 20
15min 15min 15min

AChE + BUFFER C

MgCl2.6H20 (0,08M molaridad 0.08 0.02 2.5
NaCl (0,4 M molaridad solucio 0.4 0.1 2.5
Tris (200mM molaridad solucio 0.2 0.05 2.5

10mL
Agua ultrapura 2.5
1 2 3 4 5
P:4 3 P:3

CTRL_DONP:4 = 5uM
DONP:3 = 10 uM
DONP:2 = 50 uM
DONP:1 = 100 uM
tica_G5.xpt
1 2 3 4 5
BLK BLK BLK ACT_ENZ:4 ACT_ENZ:4
15 15
P:4 3 P:3
5 5 5 10 10
T° Read 1:412 A1 A2 A3 A4
25.2 0.249 0.247 0.248 0.272
25.2 0.249 0.248 0.247 0.276
25.1 0.25 0.25 0.25 0.28
25.1 0.251 0.252 0.25 0.285
T° Read 2:412 A1 A2 A3 A4
25.1 0.262 0.26 0.262 0.565
25.2 0.264 0.263 0.264 0.664
25.2 0.267 0.265 0.265 0.758
25.2 0.269 0.267 0.268 0.836
25.2 0.27 0.269 0.272 0.926
25.2 0.272 0.272 0.274 1.02
25.2 0.274 0.274 0.274 1.119
25.3 0.277 0.277 0.276 1.231
25.3 0.28 0.279 0.283 1.292
25.3 0.282 0.283 0.281 1.396
25.3 0.284 0.286 0.284 1.486
25.3 0.287 0.287 0.286 1.572
25.3 0.29 0.289 0.288 1.677
25.4 0.292 0.292 0.291 1.795
25.4 0.295 0.294 0.293 1.898
25.4 0.297 0.296 0.295 1.988
A1 A2 A3 A4 A5
0.001 -0.001 0.001 0.304 0.302
0 -0.001 0 0.401 0.379
0.001 0 -0.001 0.492 0.456
0.001 -0.001 0 0.568 0.525
-0.001 -0.001 0.002 0.656 0.595
-0.001 -0.001 0.001 0.747 0.665
0 0 0 0.845 0.732
0.001 0 -0.001 0.954 0.808
-0.001 -0.002 0.003 1.012 0.863
0 0.001 -0.001 1.115 0.929
0 0.001 -0.001 1.202 1
0.001 0 -0.001 1.285 1.064
0.001 0 -0.001 1.388 1.119
0.001 0 -0.001 1.503 1.183
0.001 0 -0.001 1.604 1.247
0.001 0 -0.001 1.692 1.311
1 2 3 4 5
-1.7 2.833 -3.967 115.533 77.167
1 1 1 1 1
0:07:45 0:08:45 0:08:45 0:12:45 0:01:45
0:07:17 0:08:24 0:08:46 0:02:52 0:00:15
28.3 41.4 16.867 25.5 48.233
1 1 1 1 1
0:00:45 0:00:45 0:01:45 0:00:45 0:08:45
0:00:15 0:00:15 0:00:17 0:00:15 0:01:35
6 7 8 9 10
P:3 P:2 P:1
6 7 8 9 10
ACT_ENZ:4 ACT_ENZ:5 ACT_ENZ:5 ACT_ENZ:5
15 20 20 20
P:3 P:2 P:1
10 50 50 50 100
A5 A6 A7 A8 A9
0.269 0.265 0.259 0.255 0.258
0.274 0.271 0.265 0.26 0.264
0.28 0.277 0.271 0.266 0.27
0.285 0.283 0.278 0.272 0.277
A5 A6 A7 A8 A9
0.564 0.61 0.484 0.503 0.561
0.643 0.727 0.555 0.583 0.657
0.722 0.836 0.62 0.655 0.755
0.793 0.919 0.676 0.728 0.853
0.865 1.009 0.743 0.808 0.946
0.938 1.103 0.807 0.886 1.023
1.006 1.2 0.868 0.96 1.1
1.085 1.353 0.94 1.037 1.155
1.143 1.423 1 1.112 1.234
1.211 1.53 1.053 1.195 1.326
1.285 1.681 1.123 1.27 1.384
1.351 1.757 1.188 1.334 1.473
1.408 1.806 1.228 1.424 1.635
1.475 1.913 1.277 1.482 1.775
1.541 2.044 1.336 1.553 1.935
1.606 2.179 1.397 1.628 2.075
A6 A7 A8 A9 B1
0.348 0.223 0.241 0.299 0.139
0.463 0.292 0.319 0.393 0.167
0.571 0.354 0.389 0.489 0.193
0.651 0.408 0.46 0.585 0.213
0.739 0.473 0.538 0.675 0.233
0.83 0.534 0.614 0.75 0.253
0.926 0.594 0.686 0.826 0.272
1.076 0.663 0.761 0.878 0.291
1.142 0.72 0.831 0.954 0.305
1.249 0.771 0.913 1.045 0.322
1.396 0.838 0.985 1.099 0.338
1.471 0.901 1.048 1.186 0.353
1.517 0.939 1.135 1.346 0.365
1.622 0.985 1.19 1.483 0.379
1.75 1.042 1.259 1.642 0.393
1.883 1.101 1.332 1.779 0.407
6 7 8 9 10
149.7 69.2 87.4 159.5
1 1 1 1
0:06:45 0:06:45 0:11:45 0:11:45
0:02:23 0:00:54 0:02:01 0:05:42
5.633 114 20.467 20.9 13.8
1 1 1 1 1
0:08:45 0:09:45 0:01:45 0:13:45 0:04:45
0:02:54 0:02:30 0:00:25 0:01:51 0:00:37
11 12
CTRL_DON
CTRL_DONP:1
P:1
11 12
Well ID
Conc/Dil
CTRL_DON
CTRL_DONP:1 Well ID
P:1
100 100 Conc/Dil
Well ID
Conc/Dil
Well ID
Conc/Dil
Well ID
Conc/Dil
Well ID
Conc/Dil
Well ID
Conc/Dil
Well ID
Conc/Dil
B1 B2 B3 B4 B5 B6
0.265 0.262 0.232 0.239 0.255 0.241
0.271 0.269 0.233 0.245 0.264 0.247
0.277 0.275 0.235 0.249 0.27 0.253
0.284 0.282 0.236 0.255 0.277 0.259
B1 B2 B3 B4 B5 B6
0.4 0.419 0.306 0.36 0.396 0.288
0.431 0.463 0.325 0.388 0.437 0.294
0.458 0.502 0.344 0.415 0.475 0.301
0.481 0.54 0.361 0.442 0.517 0.306
0.503 0.579 0.376 0.468 0.561 0.313
0.526 0.617 0.391 0.494 0.606 0.32
0.546 0.652 0.405 0.519 0.649 0.325
0.568 0.692 0.419 0.545 0.698 0.332
0.585 0.723 0.432 0.569 0.737 0.337
0.604 0.761 0.446 0.592 0.787 0.344
0.623 0.8 0.459 0.617 0.834 0.351
0.64 0.833 0.47 0.64 0.88 0.358
0.654 0.862 0.48 0.661 0.925 0.363
0.67 0.9 0.491 0.683 0.971 0.369
0.687 0.937 0.501 0.704 1.009 0.375
0.703 0.971 0.511 0.725 1.057 0.381
B2 B3 B4 B5 B6 B7
0.158 0.045 0.099 0.135 0.026 0.252
0.199 0.062 0.124 0.173 0.031 0.344
0.237 0.078 0.149 0.21 0.035 0.441
0.272 0.093 0.173 0.249 0.038 0.526
0.308 0.106 0.197 0.29 0.042 0.609
0.344 0.118 0.221 0.333 0.047 0.694
0.378 0.131 0.245 0.376 0.051 0.778
0.416 0.143 0.268 0.421 0.056 0.892
0.443 0.152 0.288 0.457 0.057 0.946
0.479 0.164 0.311 0.505 0.062 1.021
0.515 0.174 0.332 0.55 0.066 1.136
0.546 0.183 0.354 0.593 0.071 1.201
0.573 0.191 0.372 0.636 0.074 1.28
0.608 0.199 0.391 0.679 0.077 1.379
0.643 0.207 0.41 0.715 0.081 1.471
0.675 0.215 0.43 0.761 0.085 1.548
11 12

15.233 14.2 Max V [Blank Read 2:412]

4,000
3,500
3,000
2,500
OD
2,000
2,500
OD
2,000
1,500
1,000
0,500
0,000
0:00:00
B7 B8 B9 B10 B11 B12

0.267 0.261 0.257 0.251 0.258 0.255
0.272 0.269 0.267 0.256 0.264 0.263
0.278 0.276 0.274 0.261 0.268 0.268
0.284 0.283 0.28 0.266 0.274 0.275
B7 B8 B9 B10 B11 B12
0.513 0.348 0.352 0.322 0.318 0.327
0.608 0.367 0.373 0.337 0.331 0.341
0.706 0.39 0.393 0.352 0.344 0.355
0.794 0.41 0.415 0.366 0.356 0.37
0.879 0.429 0.436 0.381 0.368 0.382
0.967 0.449 0.457 0.397 0.382 0.395
1.052 0.469 0.476 0.41 0.394 0.409
1.169 0.491 0.499 0.424 0.406 0.421
1.227 0.509 0.516 0.438 0.416 0.435
1.303 0.529 0.537 0.453 0.43 0.448
1.42 0.551 0.559 0.468 0.442 0.465
1.488 0.571 0.58 0.482 0.452 0.479
1.569 0.589 0.6 0.496 0.464 0.492
1.671 0.61 0.621 0.509 0.481 0.507
1.765 0.631 0.644 0.522 0.492 0.52
1.844 0.65 0.665 0.534 0.502 0.534
B8 B9 B10 B11 B12

0.086 0.091 0.06 0.057 0.066
0.104 0.109 0.073 0.067 0.077
0.124 0.127 0.086 0.078 0.089
0.142 0.147 0.098 0.088 0.102
0.159 0.165 0.11 0.098 0.112
0.176 0.185 0.124 0.109 0.122
0.195 0.202 0.136 0.12 0.135
0.215 0.222 0.148 0.129 0.145
0.228 0.236 0.158 0.135 0.154
0.247 0.255 0.171 0.148 0.166
0.266 0.274 0.183 0.157 0.18
0.285 0.293 0.196 0.165 0.193
0.3 0.311 0.207 0.175 0.203
0.318 0.329 0.218 0.19 0.215
0.337 0.35 0.228 0.198 0.226
0.355 0.369 0.239 0.207 0.238
4,000
3,500
3,000
2,500
2,000
2,500
2,000
1,500
1,000
0,500
0,000
0:00:00 0:10:00 0:20:00 0:30:00 0:40:00 0:5
Time
D10-Read 2:412
D11-Read 2:412
D12-Read 2:412
D10-Read 2:412
D11-Read 2:412
D12-Read 2:412
F4-Read 2:412
F7-Read 2:412
F10-Read 2:412
0:40:00 0:50:00 1:00:00 1:10:00

e
10-Read 2:412
11-Read 2:412
12-Read 2:412
10-Read 2:412
11-Read 2:412
12-Read 2:412
4-Read 2:412
7-Read 2:412
10-Read 2:412
Método 6,
ORDEN FILA A
1 BUFFER C pH 8
2 MeOH:H20/EXTRACTO
5 DONEPEZILO
A
B
C
D
E
F
G
H
OBSERVACIONES:

CTRL_DONP:4 = 5uM
DONP:3 = 10 uM
DONP:2 = 50 uM
DONP:1 = 100 uM

Date 5/3/2022
Time 19:02:01
Reading Type Reader
Procedure Details
Full Plate
Wavelengths: 412
End Kinetic
Full Plate
Wavelengths: 412
End Kinetic
Layout
Read 1:412
Time
0:00:15
0:01:15
0:02:15
0:03:15
Read 2:412
Time
0:00:15
0:01:15
0:02:15
0:03:15
0:04:15
0:05:15
0:06:15
0:07:15
0:08:15
0:09:15
0:10:15
0:11:15
0:12:15
0:13:15
0:14:15
0:15:15
Blank Read 2:412
Time
0:00:15
0:01:15
0:02:15
0:03:15
0:04:15
0:05:15
0:06:15
0:07:15
0:08:15
0:09:15
0:10:15
0:11:15
0:12:15
0:13:15
0:14:15
0:15:15
Results
A
A
H
CTRL_DON
BLK ACT_ENZ P
80 40 58
100 100 100
- 20 20
- - 2
3 mins
- 20 20
15min 15min 15min

AChE + BUFFER C


10mL
Agua ultrapura 2.5
1 2 3 4 5

P:4 3 P:3

CTRL_DONP:4 = 5uM
DONP:3 = 10 uM
DONP:2 = 50 uM
DONP:1 = 100 uM
tica_G4.xpt
1 2 3 4 5

15 15
P:4 3 P:3
5 5 5 10 10
T° Read 1:412 D1 D2 D3 D4
25.6 0.171 0.187 0.174 0.224
25.6 0.175 0.182 0.174 0.23
25.6 0.177 0.183 0.177 0.235
25.6 0.18 0.177 0.179 0.242
T° Read 2:412 D1 D2 D3 D4
25.7 0.185 0.183 0.185 0.745
25.7 0.188 0.185 0.187 0.828
25.7 0.191 0.187 0.189 0.958
25.8 0.192 0.189 0.191 1.05
25.8 0.193 0.196 0.193 1.127
25.8 0.195 0.192 0.195 1.271
25.8 0.197 0.194 0.197 1.44
25.8 0.2 0.195 0.198 1.619
25.9 0.201 0.202 0.2 1.771
25.9 0.202 0.199 0.203 1.939
25.9 0.204 0.201 0.204 2.125
25.9 0.206 0.203 0.206 2.303
26 0.208 0.208 0.207 2.48
26 0.209 0.206 0.209 2.612
26 0.211 0.208 0.211 2.728
26.1 0.213 0.21 0.213 2.87
D1 D2 D3 D4 D5
0.001 -0.002 0.001 0.561 0.582
0.001 -0.002 0.001 0.641 0.762
0.002 -0.002 0 0.769 0.986
0.001 -0.002 0.001 0.859 1.13
-0.001 0.002 -0.001 0.934 1.289
0.001 -0.002 0.001 1.077 1.446
0.001 -0.002 0.001 1.244 1.606
0.002 -0.002 0 1.422 1.745
0 0.001 -0.001 1.57 1.881
0.001 -0.002 0.002 1.737 2.003
0.001 -0.002 0.001 1.922 2.157
0.001 -0.002 0.001 2.098 2.295
0 0 0 2.273 2.447
0.001 -0.002 0.001 2.404 2.576
0.001 -0.002 0.001 2.518 2.687
0.001 -0.002 0.001 2.658 2.812
1 2 3 4 5
-2.167 3.533 2.4 184.7 224.2
1 1 1 1 1
0:07:45 0:03:45 0:08:45 0:09:45 0:01:45
0:07:45 0:03:18 0:08:55 0:02:53 0:00:27
108.3 173.733 178 129 91.3
1 1 1 1 1
0:00:45 0:03:45 0:00:45 0:00:45 0:00:45
0:00:15 0:01:07 0:00:15 0:00:15 0:00:15
6 7 8 9 10

P:3 P:2 P:1
6 7 8 9 10

15 15 15 15
P:3 P:2 P:1
10 50 50 50 100
D5 D6 D7 D8 D9
0.208 0.217 0.219 0.216 0.222
0.212 0.222 0.23 0.222 0.227
0.22 0.226 0.235 0.226 0.228
0.226 0.232 0.24 0.227 0.231
D5 D6 D7 D8 D9
0.766 0.804 0.642 0.684 0.9
0.949 0.924 0.775 0.879 1.113
1.175 1.047 0.929 1.125 1.299
1.321 1.156 1.098 1.406 1.542
1.482 1.262 1.225 1.676 1.74
1.64 1.384 1.347 1.849 1.897
1.801 1.496 1.44 2.006 1.99
1.942 1.608 1.636 2.153 2.115
2.082 1.716 1.894 2.295 2.223
2.204 1.812 2.19 2.45 2.322
2.36 1.913 2.465 2.612 2.409
2.499 2.005 2.664 2.738 2.583
2.655 2.102 2.85 2.87 2.742
2.785 2.192 2.948 2.98 2.822
2.897 2.277 3.096 3.11 2.963
3.024 2.366 3.195 3.231 3.105
D6 D7 D8 D9 E1
0.619 0.458 0.5 0.716 0.356
0.738 0.588 0.693 0.926 0.464
0.858 0.741 0.936 1.11 0.561
0.966 0.907 1.215 1.351 0.652
1.068 1.031 1.482 1.546 0.732
1.19 1.153 1.655 1.704 0.833
1.3 1.244 1.811 1.795 0.925
1.41 1.438 1.955 1.918 1.018
1.516 1.693 2.094 2.022 1.11
1.611 1.989 2.249 2.121 1.199
1.71 2.262 2.409 2.206 1.287
1.8 2.459 2.533 2.378 1.378
1.894 2.643 2.662 2.534 1.465
1.984 2.74 2.772 2.614 1.555
2.067 2.886 2.9 2.753 1.646
2.155 2.983 3.019 2.893 1.727
6 7 8 9 10
122.1 296.1 279.033 241.233
1 1 1 1
0:04:45 0:08:45 0:02:45 0:02:45
0:00:35 0:04:05 0:00:41 0:00:37
89 96.9 55.7 62 30.733
1 1 1 1 1
0:00:45 0:00:45 0:00:45 0:01:45 0:02:45
0:00:15 0:00:15 0:00:15 0:00:21 0:00:26
11 12
CTRL_DON
CTRL_DONP:1
P:1
11 12
Well ID
Conc/Dil
Well ID
Conc/Dil
Well ID
Conc/Dil
Well ID
Conc/Dil
CTRL_DON
CTRL_DONP:1 Well ID
P:1
100 100 Conc/Dil
Well ID
Conc/Dil
Well ID
Conc/Dil
Well ID
Conc/Dil
E1 E2 E3 E4 E5 E6
0.212 0.217 0.21 0.22 0.213 0.209
0.217 0.223 0.214 0.226 0.22 0.216
0.223 0.23 0.217 0.234 0.227 0.222
0.229 0.237 0.224 0.24 0.233 0.226
E1 E2 E3 E4 E5 E6
0.54 0.549 0.572 0.489 0.477 0.394
0.651 0.705 0.752 0.621 0.57 0.485
0.75 0.803 0.828 0.738 0.645 0.547
0.843 0.926 0.967 0.828 0.72 0.627
0.925 1.103 1.07 0.928 0.799 0.696
1.027 1.272 1.207 1.028 0.883 0.749
1.121 1.433 1.309 1.126 0.959 0.822
1.215 1.544 1.436 1.222 1.036 0.878
1.311 1.66 1.545 1.301 1.108 0.939
1.4 1.742 1.648 1.388 1.183 0.999
1.49 1.817 1.76 1.476 1.253 1.055
1.583 1.965 1.849 1.556 1.324 1.114
1.673 2.115 1.969 1.628 1.4 1.18
1.763 2.227 2.054 1.706 1.474 1.235
1.856 2.343 2.186 1.796 1.544 1.296
1.939 2.419 2.254 1.87 1.614 1.351
E2 E3 E4 E5 E6 E7
0.365 0.388 0.305 0.292 0.209 0.28
0.518 0.566 0.434 0.384 0.298 0.377
0.614 0.639 0.549 0.457 0.358 0.445
0.735 0.777 0.637 0.529 0.436 0.535
0.909 0.876 0.734 0.605 0.502 0.615
1.078 1.014 0.834 0.689 0.555 0.702
1.237 1.113 0.93 0.763 0.626 0.779
1.347 1.239 1.025 0.838 0.681 0.859
1.459 1.344 1.1 0.907 0.738 0.933
1.54 1.447 1.186 0.982 0.798 1.008
1.614 1.557 1.273 1.051 0.852 1.084
1.76 1.644 1.351 1.119 0.909 1.153
1.907 1.762 1.42 1.192 0.972 1.221
2.019 1.846 1.498 1.266 1.026 1.29
2.133 1.976 1.586 1.334 1.086 1.362
2.207 2.042 1.658 1.403 1.139 1.431
11 12

27.6 32.8 Max V [Blank Read 2:412]

E7 E8 E9 E10 E11 E12
0.21 0.205 0.207 0.216 0.2 0.207
0.216 0.212 0.217 0.222 0.206 0.213
0.221 0.219 0.223 0.23 0.213 0.22
0.228 0.225 0.229 0.24 0.22 0.226
E7 E8 E9 E10 E11 E12
0.464 0.376 0.382 0.324 0.298 0.319
0.563 0.434 0.44 0.353 0.326 0.354
0.634 0.484 0.504 0.385 0.354 0.385
0.725 0.536 0.561 0.417 0.382 0.418
0.809 0.592 0.608 0.447 0.409 0.45
0.896 0.646 0.668 0.478 0.437 0.481
0.975 0.697 0.722 0.508 0.464 0.512
1.056 0.747 0.776 0.538 0.491 0.542
1.134 0.793 0.832 0.567 0.515 0.571
1.21 0.842 0.883 0.596 0.542 0.602
1.287 0.885 0.935 0.622 0.567 0.63
1.357 0.928 0.983 0.65 0.592 0.661
1.429 0.975 1.034 0.677 0.618 0.69
1.498 1.022 1.085 0.706 0.643 0.72
1.572 1.067 1.136 0.733 0.669 0.748
1.643 1.112 1.187 0.759 0.693 0.777
E8 E9 E10 E11 E12

0.192 0.198 0.14 0.114 0.134
0.247 0.253 0.166 0.14 0.167
0.295 0.315 0.196 0.165 0.197
0.345 0.37 0.226 0.191 0.228
0.398 0.414 0.253 0.215 0.256
0.452 0.474 0.284 0.243 0.287
0.501 0.526 0.312 0.268 0.316
0.549 0.579 0.34 0.293 0.344
0.592 0.631 0.366 0.314 0.37
0.64 0.682 0.394 0.341 0.4
0.682 0.732 0.42 0.364 0.428
0.723 0.778 0.445 0.387 0.456
0.767 0.826 0.469 0.41 0.482
0.814 0.877 0.497 0.435 0.512
0.857 0.926 0.523 0.459 0.538
0.901 0.975 0.547 0.481 0.565
4,000
3,500
3,000
3,000
2,500
OD
2,000
1,500
1,000
0,500
0,000
0:00:00
4,000
3,500
3,000
3,000
2,500
2,000
1,500
1,000
0,500
0,000
0:00:00 0:10:00 0:20:00 0:30:00 0:40:00 0:5
Time
D10-Read 2:412
D11-Read 2:412
D12-Read 2:412
F4-Read 2:412
F7-Read 2:412
F10-Read 2:412
0:40:00 0:50:00 1:00:00 1:10:00

e
10-Read 2:412
11-Read 2:412
12-Read 2:412
4-Read 2:412
7-Read 2:412
10-Read 2:412
Método 6,
ORDEN FILA A
1 BUFFER C pH 8
2 MeOH:H20/EXTRACTO
5 DONEPEZILO
A
B
C
D
E
F
G
H
OBSERVACIONES:
Concentraciones iniciales inhibibdor Donepe

CTRL_DONP:4 = 5uM
DONP:3 = 10 uM
DONP:2 = 50 uM
DONP:1 = 100 uM

Date 5/3/2022
Time 18:15:00
Reading Type Reader
Procedure Details
Full Plate
Wavelengths: 412
End Kinetic
Full Plate
Wavelengths: 412
End Kinetic
Layout
Read 1:412
Time
0:00:15
0:01:15
0:02:15
0:03:15
Read 2:412
Time
0:00:15
0:01:15
0:02:15
0:03:15
0:04:15
0:05:15
0:06:15
0:07:15
0:08:15
0:09:15
0:10:15
0:11:15
0:12:15
0:13:15
0:14:15
0:15:15
Blank Read 2:412
Time
0:00:15
0:01:15
0:02:15
0:03:15
0:04:15
0:05:15
0:06:15
0:07:15
0:08:15
0:09:15
0:10:15
0:11:15
0:12:15
0:13:15
0:14:15
0:15:15
Results
C
C
H
CTRL_DON
BLK ACT_ENZ P
80 40 58
100 100 100
- 20 20
- - 2
3 mins
- 20 20
15min 15min 15min

ATCh + PBS (4.3mg en 1mL=15mM) 4.3 1000 4.30
DTNB + BUFFER C (1.19 mg en 1 mL= 5.95 5000 1.19
AChE + BUFFER C


10mL
Agua ultrapura 2.5
1 2 3 4 5
BLK1 BLK1 BLK1 ACT_ENZ ACT_ENZ
P:1 2 P:2
CTRL_DONP:4 = 5uM
DONP:3 = 10 uM
DONP:2 = 50 uM
DONP:1 = 100 uM
tica_G3.xpt
1 2 3 4 5
15 15
P:1 2 P:2
100 100 100 50 50
T° Read 1:412 A1 A2 A3 A4
25.1 0.228 0.227 0.21 0.254
25 0.225 0.231 0.214 0.258
25 0.231 0.234 0.212 0.262
25.1 0.233 0.236 0.214 0.267
T° Read 2:412 A1 A2 A3 A4
25.1 0.244 0.243 0.223 0.849
25 0.246 0.246 0.225 1.066
25 0.249 0.248 0.227 1.327
25 0.251 0.251 0.23 1.532
25.1 0.254 0.253 0.232 1.757
25.1 0.256 0.255 0.234 1.927
25.1 0.258 0.258 0.236 2.162
25.1 0.26 0.261 0.238 2.301
25.2 0.263 0.263 0.241 2.555
25.2 0.265 0.265 0.243 2.7
25.2 0.268 0.267 0.245 2.899
25.2 0.27 0.27 0.248 3.164
25.2 0.272 0.272 0.25 3.373
25.2 0.275 0.275 0.252 3.581
25.3 0.277 0.277 0.254 3.721
25.3 0.279 0.28 0.256 3.821
A1 A2 A3 A4 A5
0.007 0.007 -0.014 0.612 0.673
0.007 0.007 -0.014 0.827 0.877
0.007 0.007 -0.014 1.085 1.115
0.007 0.007 -0.014 1.288 1.356
0.007 0.007 -0.014 1.511 1.586
0.007 0.007 -0.014 1.679 1.761
0.007 0.007 -0.014 1.911 1.993
0.007 0.008 -0.015 2.048 2.151
0.007 0.007 -0.015 2.3 2.422
0.007 0.007 -0.015 2.442 2.647
0.007 0.007 -0.015 2.639 2.926
0.007 0.008 -0.015 2.901 3.174
0.007 0.007 -0.015 3.109 3.332
0.008 0.008 -0.015 3.314 3.472
0.007 0.008 -0.015 3.451 3.534
0.008 0.008 -0.016 3.55 3.585
1 2 3 4 5
0.6 0.467 -0.5 261.933 279.4
1 1 1 1 1
0:03:45 0:05:45 0:12:45 0:10:45 0:09:45
0:03:42 0:04:49 0:10:39 0:02:31 0:02:11
249.533 152.733 300.067 171.033 144.333
1 1 1 1 1
0:13:45 0:08:45 0:07:45 0:08:45 0:10:45
0:06:19 0:02:11 0:03:40 0:03:08 0:03:04
6 7 8 9 10
P:2 P:3 P:4
6 7 8 9 10
15 15 15 15
P:2 P:3 P:4
50 10 10 10 5
A5 A6 A7 A8 A9
0.247 0.241 0.237 0.24 0.231
0.251 0.243 0.242 0.243 0.236
0.257 0.248 0.247 0.247 0.24
0.262 0.254 0.251 0.251 0.244
A5 A6 A7 A8 A9
0.91 0.632 1.067 0.929 0.855
1.116 0.793 1.309 1.214 1.087
1.357 0.945 1.558 1.51 1.263
1.599 1.091 1.862 1.75 1.563
1.833 1.268 2.166 2.029 1.759
2.009 1.399 2.43 2.312 1.968
2.244 1.654 2.688 2.575 2.253
2.404 1.819 2.925 2.828 2.463
2.677 2.273 3.138 3.051 2.675
2.905 2.225 3.33 3.237 2.86
3.186 2.617 3.488 3.394 3.046
3.437 3.031 3.61 3.552 3.242
3.597 3.232 3.693 3.64 3.398
3.739 3.411 3.773 3.731 3.514
3.803 3.518 3.823 3.768 3.621
3.857 3.626 3.839 3.809 3.673
A6 A7 A8 A9 B1
0.395 0.83 0.692 0.618 0.322
0.554 1.07 0.975 0.848 0.455
0.704 1.316 1.269 1.022 0.489
0.847 1.618 1.507 1.32 0.696
1.022 1.92 1.783 1.513 0.775
1.15 2.182 2.063 1.72 0.899
1.403 2.437 2.325 2.002 1.06
1.566 2.672 2.575 2.21 1.177
2.017 2.882 2.795 2.42 1.315
1.967 3.072 2.979 2.602 1.426
2.357 3.228 3.134 2.785 1.569
2.768 3.348 3.289 2.979 1.642
2.967 3.428 3.376 3.134 1.868
3.144 3.506 3.463 3.246 2.054
3.248 3.553 3.498 3.352 2.303
3.354 3.567 3.537 3.401 2.447
6 7 8 9 10
450.967 302.367 293.733 297.867
1 1 1 1
0:07:45 0:02:45 0:01:45 0:02:45
0:04:39 0:00:39 0:00:17 0:00:54
118.533 162.633 41.533 100.133 34.833
1 1 1 1 1
0:10:45 0:08:45 0:00:45 0:08:45 0:10:45
0:01:33 0:03:37 0:00:15 0:02:56 0:01:45
11 12
CTRL_DON
CTRL_DONP:4
P:4
11 12
Well ID
Conc/Dil
CTRL_DON
CTRL_DONP:4 Well ID
P:4
5 5 Conc/Dil
Well ID
Conc/Dil
Well ID
Conc/Dil
Well ID
Conc/Dil
Well ID
Conc/Dil
Well ID
Conc/Dil
Well ID
Conc/Dil
B1 B2 B3 B4 B5 B6
0.254 0.237 0.223 0.231 0.245 0.243
0.256 0.241 0.225 0.235 0.25 0.248
0.262 0.246 0.229 0.239 0.253 0.253
0.268 0.251 0.234 0.243 0.258 0.258
B1 B2 B3 B4 B5 B6
0.559 0.462 0.592 0.581 0.538 0.52
0.694 0.57 0.755 0.685 0.65 0.635
0.731 0.693 0.981 0.794 0.759 0.743
0.94 0.824 1.099 0.907 0.869 0.859
1.021 0.935 1.235 1.006 0.977 0.959
1.148 1.042 1.31 1.156 1.061 1.062
1.311 1.149 1.54 1.251 1.185 1.181
1.43 1.282 1.682 1.388 1.273 1.276
1.571 1.406 1.984 1.476 1.417 1.379
1.684 1.561 2.087 1.649 1.48 1.47
1.829 1.695 2.29 1.742 1.597 1.576
1.905 1.821 2.534 1.829 1.744 1.697
2.133 1.932 2.714 1.952 1.869 1.805
2.321 2.032 2.905 2.075 2.014 1.924
2.572 2.127 3.007 2.224 2.152 2.037
2.719 2.228 3.236 2.333 2.293 2.148
B2 B3 B4 B5 B6 B7
0.225 0.355 0.345 0.302 0.284 0.258
0.331 0.516 0.446 0.411 0.396 0.369
0.452 0.74 0.553 0.518 0.502 0.467
0.58 0.856 0.664 0.625 0.615 0.553
0.689 0.989 0.76 0.731 0.713 0.645
0.794 1.062 0.907 0.813 0.814 0.765
0.898 1.289 1 0.934 0.931 0.843
1.029 1.428 1.135 1.02 1.023 0.969
1.151 1.729 1.22 1.162 1.124 1.011
1.303 1.83 1.391 1.222 1.212 1.174
1.435 2.03 1.482 1.337 1.316 1.282
1.559 2.271 1.567 1.482 1.434 1.37
1.668 2.45 1.687 1.605 1.541 1.532
1.765 2.638 1.807 1.747 1.657 1.676
1.858 2.738 1.954 1.883 1.767 1.769
1.956 2.964 2.062 2.021 1.877 1.867
11 12
33.567 31.433 Max V [Blank Read 2:412]


4,000
3,500
3,000
2,500
OD
2,000
1,500
1,000
0,500
1,000
0,500
0,000
0:00:00
B7 B8 B9 B10 B11 B12

0.206 0.209 0.244 0.261 0.254 0.252
0.211 0.212 0.248 0.263 0.254 0.257
0.217 0.216 0.254 0.267 0.261 0.261
0.218 0.221 0.259 0.271 0.266 0.266
B7 B8 B9 B10 B11 B12

0.494 0.327 0.423 0.334 0.332 0.335
0.608 0.371 0.491 0.366 0.358 0.369
0.709 0.41 0.571 0.399 0.385 0.394
0.796 0.454 0.626 0.432 0.418 0.427
0.891 0.492 0.691 0.464 0.444 0.458
1.013 0.531 0.757 0.491 0.474 0.489
1.094 0.572 0.816 0.525 0.51 0.519
1.222 0.61 0.872 0.556 0.536 0.545
1.267 0.65 0.974 0.589 0.572 0.574
1.432 0.687 1.076 0.617 0.6 0.602
1.542 0.724 1.171 0.653 0.631 0.629
1.632 0.761 1.251 0.69 0.661 0.654
1.796 0.799 1.323 0.72 0.688 0.679
1.943 0.836 1.384 0.751 0.715 0.703
2.039 0.874 1.462 0.785 0.738 0.731
2.138 0.911 1.524 0.817 0.767 0.755
B8 B9 B10 B11 B12

0.09 0.186 0.097 0.096 0.098
0.132 0.253 0.127 0.12 0.13
0.169 0.329 0.158 0.144 0.153
0.21 0.383 0.188 0.174 0.184
0.246 0.445 0.218 0.198 0.212
0.283 0.509 0.243 0.226 0.24
0.322 0.565 0.275 0.259 0.268
0.356 0.619 0.303 0.283 0.292
0.394 0.718 0.334 0.317 0.319
0.429 0.818 0.36 0.342 0.344
0.464 0.911 0.393 0.371 0.369
0.499 0.988 0.428 0.398 0.392
0.534 1.059 0.455 0.423 0.414
0.569 1.117 0.484 0.448 0.436
0.605 1.193 0.515 0.469 0.461
0.639 1.252 0.545 0.495 0.483
4,000
3,500
3,000
2,500
2,000
1,500
1,000
0,500
1,000
0,500
0,000
0:00:00 0:10:00 0:20:00 0:30:00 0:40:00 0:5
Time
D10-Read 2:412
D11-Read 2:412
D12-Read 2:412
F4-Read 2:412
F7-Read 2:412
F10-Read 2:412
0:40:00 0:50:00 1:00:00 1:10:00
e
10-Read 2:412
11-Read 2:412
12-Read 2:412
4-Read 2:412
7-Read 2:412
10-Read 2:412
Método 6,
ORDEN FILA A
1 BUFFER C pH 8
2 MeOH:H20/EXTRACTO
5 DONEPEZILO
A
B
C
D
E
F
G
H
OBSERVACIONES:

CTRL_DONP:4 = 5uM
DONP:3 = 10 uM
DONP:2 = 50 uM
DONP:1 = 100 uM

Date 4/26/2022
Time 18:15:54
Reading Type Reader
Procedure Details
Full Plate
Wavelengths: 412
End Kinetic
Full Plate
Wavelengths: 412
End Kinetic
Layout
Read 1:412
Time
0:00:15
0:01:15
0:02:15
0:03:15
Read 2:412
Time
0:00:15
0:01:15
0:02:15
0:03:15
0:04:15
0:05:15
0:06:15
0:07:15
0:08:15
0:09:15
0:10:15
0:11:15
0:12:15
0:13:15
0:14:15
0:15:15
Blank Read 2:412
Time
0:00:15
0:01:15
0:02:15
0:03:15
0:04:15
0:05:15
0:06:15
0:07:15
0:08:15
0:09:15
0:10:15
0:11:15
0:12:15
0:13:15
0:14:15
0:15:15
Results
B
C
H
CTRL_DON
BLK ACT_ENZ P
80 40 58
100 100 100
- 20 20
- - 2
3 mins
- 20 20
15min 15min 15min

AChE + BUFFER C


10mL
Agua ultrapura 2.5
1 2 3 4 5
BLK1 BLK1 BLK1 ACT_ENZ ACT_ENZ
P:1 2 P:2
CTRL_DONP:4 = 5uM
DONP:3 = 10 uM
DONP:2 = 50 uM
DONP:1 = 100 uM
tica_G2.xpt
1 2 3 4 5
15 15
P:1 2 P:2
100 100 100 50 50
T° Read 1:412 A1 A2 A3 A4
25.2 0.281 0.257 0.27 0.255
25.1 0.289 0.263 0.272 0.259
25.1 0.289 0.263 0.279 0.262
25.1 0.295 0.277 0.283 0.259
T° Read 2:412 A1 A2 A3 A4
25.1 0.318 0.29 0.315 1.507
25.1 0.323 0.296 0.319 1.798
25.1 0.328 0.299 0.324 2.097
25.1 0.333 0.304 0.328 2.508
25.1 0.334 0.309 0.331 2.787
25.2 0.33 0.313 0.334 3.095
25.2 0.33 0.321 0.339 3.334
25.2 0.333 0.322 0.343 3.525
25.2 0.339 0.327 0.345 3.644
25.2 0.346 0.331 0.349 3.763
25.3 0.351 0.336 0.355 3.814
25.3 0.355 0.34 0.359 3.862
25.3 0.36 0.342 0.363 3.888
25.3 0.364 0.346 0.368 3.881
25.3 0.37 0.351 0.372 3.916
25.3 0.372 0.354 0.377 3.928
A1 A2 A3 A4 A5
0.01 -0.018 0.007 1.199 1.049
0.01 -0.017 0.007 1.485 1.336
0.011 -0.018 0.006 1.78 1.592
0.011 -0.017 0.006 2.186 1.904
0.01 -0.016 0.006 2.463 2.307
0.005 -0.013 0.008 2.77 2.649
0 -0.009 0.009 3.004 2.863
0.001 -0.011 0.01 3.192 3.107
0.002 -0.01 0.008 3.307 3.256
0.004 -0.011 0.007 3.421 3.374
0.004 -0.011 0.008 3.467 3.445
0.004 -0.011 0.008 3.511 3.49
0.005 -0.013 0.008 3.533 3.521
0.005 -0.013 0.009 3.521 3.521
0.005 -0.013 0.008 3.551 3.508
0.004 -0.013 0.009 3.56 3.568
1 2 3 4 5
-5.133 4.1 2.367 405.767 402.833
1 1 1 1 1
0:04:45 0:05:45 0:04:45 0:02:45 0:03:45
0:04:06 0:04:09 0:04:40 0:00:49 0:01:08
372.667 327.167 263.367 111.933 149.467
1 1 1 1 1
0:02:45 0:02:45 0:02:45 0:03:45 0:02:45
0:00:40 0:00:18 0:00:35 0:00:23 0:00:38
6 7 8 9 10
P:2 P:3 P:4
6 7 8 9 10
15 15 15 15
P:2 P:3 P:4
50 10 10 10 5
A5 A6 A7 A8 A9
0.26 0.25 0.246 0.249 0.243
0.264 0.254 0.25 0.253 0.246
0.268 0.257 0.251 0.257 0.25
0.27 0.26 0.251 0.26 0.252
A5 A6 A7 A8 A9
1.357 1.169 1.313 1.095 1.364
1.649 1.346 1.66 1.395 1.708
1.909 1.57 2.003 1.692 2.044
2.226 1.804 2.325 1.96 2.374
2.632 2.049 2.625 2.213 2.676
2.975 2.297 2.916 2.453 2.955
3.193 2.544 3.158 2.692 3.197
3.439 2.773 3.36 2.895 3.407
3.593 3.004 3.534 3.094 3.566
3.716 3.219 3.656 3.267 3.685
3.792 3.37 3.758 3.407 3.769
3.841 3.497 3.812 3.532 3.813
3.876 3.601 3.842 3.64 3.845
3.88 3.688 3.876 3.711 3.865
3.873 3.746 3.882 3.771 3.88
3.935 3.773 3.887 3.809 3.889
A6 A7 A8 A9 B1
0.861 1.006 0.788 1.057 0.54
1.033 1.347 1.082 1.396 0.89
1.253 1.686 1.375 1.727 1.13
1.483 2.003 1.638 2.052 1.502
1.724 2.3 1.889 2.352 1.697
1.971 2.59 2.128 2.629 1.916
2.215 2.828 2.362 2.868 2.143
2.44 3.027 2.562 3.075 2.409
2.667 3.197 2.757 3.229 2.559
2.877 3.314 2.924 3.343 2.79
3.022 3.411 3.06 3.422 2.963
3.146 3.461 3.18 3.461 3.094
3.245 3.487 3.284 3.489 3.223
3.328 3.516 3.352 3.505 3.314
3.382 3.518 3.407 3.516 3.375
3.405 3.519 3.441 3.521 3.422
6 7 8 9 10
247.067 341.433 294.233 338.933
1 1 1 1
0:04:45 0:00:45 0:00:45 0:00:45
0:00:45 0:00:15 0:00:15 0:00:15
367.833 386.133 120.167 239.267 302.5
1 1 1 1 1
0:03:45 0:00:45 0:04:45 0:02:45 0:01:45
0:00:51 0:00:15 0:00:48 0:00:18 0:00:16
11 12
CTRL_DON
CTRL_DONP:4
P:4
11 12
Well ID
Conc/Dil
CTRL_DON
CTRL_DONP:4 Well ID
P:4
5 5 Conc/Dil
Well ID
Conc/Dil
Well ID
Conc/Dil
Well ID
Conc/Dil
Well ID
Conc/Dil
Well ID
Conc/Dil
Well ID
Conc/Dil
B1 B2 B3 B4 B5 B6
0.212 0.209 0.223 0.234 0.231 0.216
0.216 0.213 0.228 0.233 0.234 0.218
0.22 0.215 0.231 0.252 0.237 0.222
0.222 0.219 0.235 0.244 0.242 0.225
B1 B2 B3 B4 B5 B6
0.848 0.992 0.842 0.555 0.517 0.917
1.203 1.323 1.072 0.666 0.653 1.17
1.447 1.642 1.291 0.776 0.769 1.482
1.824 1.974 1.559 0.891 0.923 1.814
2.021 2.302 1.788 1.006 1.068 2.185
2.242 2.621 2.011 1.117 1.212 2.47
2.473 2.916 2.215 1.224 1.363 2.769
2.741 3.176 2.428 1.335 1.502 3.111
2.896 3.394 2.61 1.433 1.621 3.324
3.132 3.573 2.812 1.539 1.744 3.508
3.31 3.691 2.99 1.641 1.873 3.627
3.446 3.768 3.153 1.738 1.956 3.72
3.578 3.819 3.298 1.832 2.064 3.797
3.674 3.862 3.43 1.929 2.168 3.842
3.74 3.873 3.523 2.018 2.262 3.875
3.79 3.9 3.625 2.112 2.368 3.897
B2 B3 B4 B5 B6 B7
0.684 0.535 0.248 0.21 0.61 0.681
1.01 0.759 0.353 0.341 0.857 1.067
1.325 0.974 0.459 0.452 1.165 1.397
1.652 1.237 0.57 0.601 1.493 1.729
1.977 1.464 0.681 0.743 1.86 2.018
2.295 1.686 0.791 0.886 2.145 2.312
2.586 1.885 0.895 1.033 2.44 2.579
2.844 2.095 1.002 1.169 2.779 2.822
3.057 2.273 1.096 1.284 2.986 3.04
3.23 2.469 1.197 1.402 3.165 3.2
3.344 2.643 1.294 1.526 3.28 3.333
3.417 2.802 1.387 1.604 3.368 3.402
3.464 2.943 1.477 1.709 3.442 3.454
3.502 3.07 1.569 1.808 3.482 3.495
3.509 3.158 1.654 1.897 3.51 3.507
3.532 3.257 1.744 2 3.529 3.559
11 12
309.567 37.967 Max V [Blank Read 2:412]


4,000
3,500
3,000
2,500
OD
2,000
1,500
1,000
1,500
1,000
0,500
0,000
0:00:00
B7 B8 B9 B10 B11 B12

0.254 0.251 0.247 0.227 0.236 0.239
0.259 0.253 0.25 0.232 0.239 0.243
0.262 0.257 0.253 0.235 0.242 0.247
0.265 0.26 0.258 0.239 0.247 0.251
B7 B8 B9 B10 B11 B12

0.989 0.64 0.803 0.897 0.91 0.351
1.38 0.748 1.045 1.2 1.217 0.394
1.714 0.85 1.278 1.507 1.516 0.434
2.05 0.963 1.522 1.812 1.83 0.475
2.342 1.071 1.732 2.074 2.121 0.514
2.638 1.192 1.936 2.361 2.394 0.554
2.909 1.302 2.139 2.621 2.653 0.591
3.155 1.388 2.335 2.845 2.897 0.627
3.377 1.498 2.52 3.053 3.109 0.665
3.542 1.581 2.714 3.251 3.317 0.703
3.68 1.696 2.889 3.413 3.473 0.74
3.753 1.785 3.047 3.548 3.601 0.775
3.809 1.894 3.188 3.653 3.702 0.811
3.855 1.984 3.315 3.722 3.771 0.846
3.872 2.065 3.433 3.768 3.802 0.883
3.927 2.158 3.54 3.82 3.822 0.918
B8 B9 B10 B11 B12

0.333 0.495 0.589 0.602 0.043
0.435 0.732 0.888 0.904 0.081
0.533 0.961 1.19 1.199 0.117
0.641 1.2 1.49 1.508 0.153
0.747 1.407 1.75 1.797 0.19
0.867 1.611 2.035 2.068 0.228
0.972 1.809 2.291 2.324 0.261
1.056 2.002 2.512 2.565 0.295
1.161 2.183 2.716 2.772 0.328
1.238 2.372 2.909 2.975 0.361
1.349 2.542 3.066 3.126 0.393
1.434 2.696 3.197 3.249 0.424
1.539 2.833 3.298 3.347 0.455
1.625 2.955 3.362 3.412 0.486
1.701 3.068 3.404 3.437 0.518
1.79 3.172 3.452 3.454 0.55
4,000
3,500
3,000
2,500
2,000
1,500
1,000
1,500
1,000
0,500
0,000
0:00:00 0:10:00 0:20:00 0:30:00 0:40:00 0:5
Time
D10-Read 2:412
D11-Read 2:412
D12-Read 2:412
F4-Read 2:412
F7-Read 2:412
F10-Read 2:412
F7-Read 2:412
F10-Read 2:412
0:40:00 0:50:00 1:00:00 1:10:00

e
10-Read 2:412
11-Read 2:412
12-Read 2:412
4-Read 2:412
7-Read 2:412
10-Read 2:412
7-Read 2:412
10-Read 2:412
Método 6,
ORDEN FILA A BLK

1 BUFFER C pH 8 80
2 MeOH:H20/EXTRACTO 20 metanol /buffer
3 DTNB (3 mM) TRIS 50mM pH8 100
4 ATCh /SUSTRATO (15mM) AGUA -
5 DONEPEZILO -
PRE-INCUBACIÓN (412nm-cinetica) 25°c 3 mins
AChE (1 U/mL) (15 ul + 1985 BUFFER C-TRIS -50mM pH8) -
Cinética 412 nm 60 min (c/1mins) 25 °C 15min
PARA LA PRUEBA
Mínimo Buffer C
ATCh + PBS (4.3mg en 1mL=
DTNB + BUFFER C (1.19 mg
AChE + BUFFER C
PARA REACTIVOS BUFFER C

1
A BLK1
B CTRL_DONP:1
C
D
E
F
G
H
OBSERVACIONES:

CTRL_DONP:4 = 5uM
DONP:3 = 10 uM
DONP:2 = 50 uM
DONP:1 = 100 uM
Experiment File Path: C:\Users\Public\Documents\Experiments\PRACTICA_G1.xpt

Date 4/19/2022
Time 18:29:12
Reading Type Reader
Procedure Details
Full Plate
Wavelengths: 412
End Kinetic
Full Plate
Wavelengths: 412
End Kinetic
Layout
1
BLK
A
CTRL_DONP:1
B
100
Read 1:412
Time T° Read 1:412

0:00:15 25.8
0:01:15 25.8
0:02:15 25.8
0:03:15 25.8
Read 2:412
Time T° Read 2:412

0:00:15 25.8
0:01:15 25.8
0:02:15 25.8
0:03:15 25.8
0:04:15 25.9
0:05:15 25.9
0:06:15 25.9
0:07:15 25.9
0:08:15 25.9
0:09:15 26
0:10:15 26
0:11:15 26
0:12:15 26
0:13:15 26.1
0:14:15 26.1
0:15:15 26.1
Blank Read 2:412
Time A1
0:00:15 -0.002
0:01:15 -0.001
0:02:15 0
0:03:15 0
0:04:15 0
0:05:15 0
0:06:15 0
0:07:15 0
0:08:15 0
0:09:15 0
0:10:15 0
0:11:15 0
0:12:15 0
0:13:15 -0.001
0:14:15 -0.001
0:15:15 -0.001
Results
1
1.467
1
A
0:00:45
0:00:15
8.067
1
B
0:00:45
0:00:15
H
CTRL_DON
ACT_ENZ P
40 58
20 metanol -
100 100
20 20
- 2
20 20
15min 15min
peso inicial uL peso final (mg/mL)

10000
8.6 2000 4.30
11.9 10 1.19
10010 PREPARAR 10 mL

10mL
Agua ultrapura 2.5
2 3 4 5 6 7
BLK1 BLK1 ACT_ENZ ACT_ENZ ACT_ENZ ACT_ENZ
CTRL_DON CTRL_DONP: CTRL_DON CTRL_DON CTRL_DON
CTRL_DONP:1
P:1 2 P:2 P:2 P:3
ciales inhibibdor Donepezilo:

DONP:4 = 5uM
NP:3 = 10 uM
NP:2 = 50 uM
P:1 = 100 uM
2 3 4 5 6 7
BLK BLK ACT_ENZ ACT_ENZ ACT_ENZ ACT_ENZ
15 15 15 15
CTRL_DON CTRL_DONP: CTRL_DON CTRL_DON CTRL_DON
CTRL_DONP:1
P:1 2 P:2 P:2 P:3
100 100 50 50 50 10
A1 A2 A3 A4 A5 A6
0.188 0.194 0.195 0.224 0.22 0.219
0.19 0.195 0.195 0.228 0.225 0.223
0.19 0.192 0.196 0.233 0.233 0.226
0.191 0.194 0.196 0.238 0.236 0.232
A1 A2 A3 A4 A5 A6
0.203 0.207 0.206 0.634 0.497 0.642
0.205 0.205 0.207 0.718 0.601 0.743
0.207 0.204 0.209 0.744 0.655 0.825
0.208 0.204 0.211 0.828 0.721 0.898
0.21 0.207 0.212 0.9 0.763 0.964
0.211 0.207 0.214 0.959 0.816 1.042
0.213 0.209 0.217 1.098 0.875 1.127
0.214 0.212 0.218 1.188 0.938 1.222
0.216 0.214 0.22 1.232 0.985 1.293
0.218 0.215 0.221 1.404 1.039 1.402
0.219 0.216 0.223 1.438 1.093 1.526
0.221 0.218 0.224 1.642 1.142 1.646
0.222 0.219 0.225 1.726 1.188 1.748
0.223 0.222 0.227 1.836 1.236 1.854
0.225 0.222 0.231 1.923 1.28 1.961
0.226 0.224 0.231 1.989 1.33 2.054
A2 A3 A4 A5 A6 A7
0.002 0.001 0.429 0.291 0.437 0.323
-0.001 0.002 0.512 0.395 0.537 0.401
-0.003 0.003 0.538 0.448 0.618 0.443
-0.003 0.003 0.62 0.513 0.69 0.519
-0.003 0.003 0.69 0.554 0.754 0.606
-0.003 0.003 0.748 0.605 0.831 0.676
-0.004 0.004 0.885 0.662 0.914 0.77
-0.003 0.003 0.973 0.723 1.007 0.843
-0.003 0.003 1.016 0.769 1.077 0.902
-0.003 0.003 1.186 0.821 1.184 0.969
-0.003 0.004 1.219 0.874 1.306 1.023
-0.003 0.003 1.42 0.92 1.425 1.07
-0.003 0.003 1.504 0.966 1.526 1.126
-0.002 0.003 1.612 1.012 1.63 1.186
-0.004 0.005 1.697 1.054 1.735 1.245
-0.003 0.004 1.762 1.103 1.827 1.295
2 3 4 5 6 7
-2.733 2.167 201.3 103.267 122.8 94.3
1 1 1 1 1 1
0:00:45 0:13:45 0:10:45 0:00:45 0:09:45 0:05:45
0:00:15 0:12:12 0:06:20 0:00:15 0:03:10 0:01:30
4.167 11.567 10.6 13.233 12.8 48.3
1 1 1 1 1 1
0:00:45 0:13:45 0:01:45 0:14:45 0:01:45 0:09:45
0:00:15 0:05:36 0:00:25 0:06:09 0:00:29 0:02:42
8 9 10 11 12
ACT_ENZ ACT_ENZ
CTRL_DON CTRL_DON
CTRL_DONP:3 CTRL_DONP:3 CTRL_DONP:4
P:4 P:4
8 9 10 11 12
ACT_ENZ ACT_ENZ
15 15
CTRL_DON CTRL_DON
CTRL_DONP:3 CTRL_DONP:3 CTRL_DONP:4
P:4 P:4
10 10 5 5 5
A7 A8 A9 B1 B2
0.235 0.228 0.226 0.216 0.222
0.239 0.234 0.229 0.219 0.227
0.243 0.235 0.233 0.224 0.231
0.248 0.24 0.239 0.226 0.235
A7 A8 A9 B1 B2
0.528 0.294 0.521 0.284 0.276
0.607 0.32 0.582 0.292 0.281
0.65 0.341 0.597 0.298 0.284
0.727 0.363 0.666 0.306 0.289
0.816 0.387 0.69 0.314 0.292
0.887 0.414 0.733 0.321 0.297
0.983 0.442 0.856 0.329 0.301
1.057 0.475 0.947 0.337 0.306
1.118 0.5 0.983 0.345 0.31
1.187 0.533 1.109 0.352 0.315
1.242 0.556 1.138 0.36 0.319
1.291 0.588 1.242 0.367 0.323
1.347 0.611 1.273 0.375 0.327
1.41 0.635 1.437 0.382 0.333
1.47 0.659 1.55 0.389 0.336
1.522 0.681 1.618 0.397 0.341
A8 A9 B1 B2 B3
0.088 0.316 0.078 0.071 0.103
0.115 0.376 0.087 0.075 0.114
0.134 0.391 0.091 0.077 0.119
0.155 0.458 0.099 0.081 0.127
0.178 0.481 0.104 0.083 0.134
0.203 0.522 0.11 0.086 0.142
0.229 0.643 0.116 0.089 0.149
0.26 0.732 0.122 0.091 0.156
0.284 0.767 0.128 0.094 0.163
0.315 0.891 0.134 0.097 0.168
0.337 0.918 0.141 0.099 0.175
0.367 1.021 0.146 0.102 0.181
0.389 1.051 0.153 0.105 0.185
0.411 1.213 0.158 0.109 0.192
0.433 1.324 0.163 0.111 0.203
0.454 1.391 0.17 0.114 0.205
8 9 10 11 12
30.7 162.267
1 1
0:06:45 0:12:45
0:01:39 0:07:43
54.767 29.9 62.6 38.9 38.333
1 1 1 1 1
0:04:45 0:09:45 0:09:45 0:01:45 0:02:45
0:00:33 0:02:35 0:02:40 0:00:23 0:00:21
Well ID
Conc/Dil
Well ID
Conc/Dil
Well ID
Conc/Dil
Well ID
Conc/Dil
Well ID
Conc/Dil
Well ID
Conc/Dil
Well ID
Conc/Dil
Well ID
Conc/Dil
B3 B4 B5 B6 B7 B8
0.221 0.212 0.206 0.208 0.217 0.222
0.226 0.218 0.21 0.213 0.223 0.227
0.231 0.221 0.214 0.216 0.225 0.228
0.234 0.225 0.219 0.22 0.229 0.233
B3 B4 B5 B6 B7 B8
0.309 0.284 0.279 0.303 0.358 0.388
0.32 0.293 0.288 0.313 0.395 0.442
0.326 0.305 0.296 0.326 0.428 0.493
0.335 0.315 0.304 0.339 0.463 0.546
0.344 0.326 0.313 0.351 0.491 0.595
0.353 0.335 0.323 0.364 0.53 0.651
0.362 0.345 0.331 0.378 0.562 0.697
0.371 0.353 0.343 0.389 0.598 0.744
0.379 0.36 0.35 0.402 0.645 0.787
0.386 0.367 0.36 0.415 0.687 0.82
0.394 0.372 0.368 0.428 0.737 0.861
0.402 0.38 0.379 0.439 0.771 0.9
0.407 0.388 0.391 0.449 0.809 0.947
0.416 0.395 0.4 0.462 0.841 0.984
0.429 0.402 0.406 0.475 0.87 1.025
0.432 0.411 0.421 0.487 0.891 1.063
B4 B5 B6 B7 B8 B9
0.079 0.073 0.097 0.153 0.183 0.129
0.088 0.082 0.107 0.189 0.236 0.149
0.098 0.089 0.12 0.221 0.286 0.177
0.107 0.096 0.131 0.255 0.338 0.201
0.116 0.103 0.141 0.281 0.385 0.218
0.124 0.113 0.153 0.319 0.44 0.243
0.132 0.118 0.165 0.349 0.484 0.259
0.139 0.128 0.174 0.383 0.53 0.281
0.144 0.133 0.185 0.429 0.571 0.308
0.149 0.142 0.197 0.469 0.602 0.328
0.153 0.149 0.208 0.517 0.641 0.358
0.159 0.158 0.218 0.55 0.679 0.381
0.166 0.169 0.227 0.587 0.725 0.407
0.171 0.176 0.238 0.617 0.76 0.437
0.177 0.181 0.249 0.644 0.799 0.464
0.184 0.194 0.26 0.665 0.836 0.486

4,000
3,500
3,000
2,500
OD
2,000
2,500
OD
2,000
1,500
1,000
0,500
0,000
0:00:00 0:10:00 0
B9 B10 B11 B12

0.22 0.22 0.22 0.221
0.224 0.225 0.222 0.225
0.227 0.227 0.225 0.228
0.23 0.233 0.23 0.232
B9 B10 B11 B12

0.334 0.363 0.332 0.339
0.355 0.393 0.366 0.375
0.384 0.447 0.406 0.413
0.409 0.503 0.445 0.453
0.427 0.546 0.479 0.489
0.454 0.594 0.512 0.526
0.472 0.634 0.544 0.56
0.496 0.678 0.581 0.594
0.524 0.733 0.621 0.633
0.546 0.788 0.659 0.662
0.577 0.851 0.692 0.7
0.602 0.904 0.726 0.727
0.629 0.951 0.756 0.762
0.661 0.999 0.793 0.794
0.689 1.046 0.82 0.827
0.713 1.087 0.855 0.856
B10 B11 B12

0.158 0.126 0.133
0.188 0.16 0.169
0.24 0.199 0.207
0.295 0.237 0.245
0.336 0.269 0.28
0.383 0.301 0.315
0.421 0.331 0.347
0.464 0.366 0.38
0.517 0.404 0.416
0.57 0.441 0.444
0.632 0.473 0.481
0.683 0.505 0.506
0.73 0.534 0.54
0.775 0.569 0.571
0.82 0.595 0.601
0.86 0.628 0.629
0:10:00 0:20:00 0:30:00 0:40:00 0:50:00 1:00
Time
D10-Read 2:412
D11-Read 2:412
D12-Read 2:412
F4-Read 2:412
D10-Read 2:412
D11-Read 2:412
D12-Read 2:412
F4-Read 2:412
F7-Read 2:412
F10-Read 2:412
:50:00 1:00:00 1:10:00

PROTOCOLO ACETILCOLINESTERASA_LABORATORIO DE BIOENSAYOS_DEPARTAMENTO DE QUÍMICA_UTPL_20
MENTO DE QUÍMICA_UTPL_2022 VISTA PREVIA RESULTADOS Gru
ISTA PREVIA RESULTADOS Grupo 8 Y VISTE GENERAL DEL PROGRAMA

Práctica Acetilcolinesterasa - Aa2022

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Práctica Acetilcolinesterasa - Aa2022

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1.

f(x) = − 56.2689075630276 x² + 12.6741428571429 x + 0.206432451418067

ACT_ENZ Polynomial (ACT_ENZ) 50uMDNPZ Polynomial (50uMDNPZ)

Concentraciones iniciales inhibibdor Donepez

Software Version 2.09.1

Experiment File Path: C:\Users\Public\Documents\Experiments\Practica_G8.xpt

Blank Read 2:412

PARA LA PRUEBA peso inicial uL peso final (mg/mL)

PARA REACTIVOS BUFFER C 15000 PREPARAR 10 mL

Ajustar a pH8 con HCl 2M (140uL) y ajustar volumen a

Concentraciones iniciales inhibibdor Donepezilo:

BLK BLK BLK ACT_ENZ ACT_ENZ

ACT_ENZ ACT_ENZ ACT_ENZ ACT_ENZ

R-Squared [Blank Read 2:412]

t at Max V [Blank Read 2:412]

Lagtime [Blank Read 2:412]

Max V [Blank Read 2:412]

R-Squared [Blank Read 2:412]

t at Max V [Blank Read 2:412]

Max V [Blank Read 2:412]

R-Squared [Blank Read 2:412]

t at Max V [Blank Read 2:412]

Lagtime [Blank Read 2:412]

Max V [Blank Read 2:412]

R-Squared [Blank Read 2:412]

t at Max V [Blank Read 2:412]

Lagtime [Blank Read 2:412]

Max V [Blank Read 2:412]

R-Squared [Blank Read 2:412]

t at Max V [Blank Read 2:412]

Lagtime [Blank Read 2:412]

Max V [Blank Read 2:412]

R-Squared [Blank Read 2:412]

t at Max V [Blank Read 2:412]

Lagtime [Blank Read 2:412]

Max V [Blank Read 2:412]

R-Squared [Blank Read 2:412]

t at Max V [Blank Read 2:412]

Lagtime [Blank Read 2:412]

8.3 8.3 Max V [Blank Read 2:412]

1 1 R-Squared [Blank Read 2:412]

0:02:45 0:06:45 t at Max V [Blank Read 2:412]

0:00:22 0:00:49 Lagtime [Blank Read 2:412]

H7 H8 H9 H10 H11 H12

H7 H8 H9 H10 H11 H12

H8 H9 H10 H11 H12

0:40:00 0:50:00 1:00:00 1:10:00

Concentraciones iniciales inhibibdor Donepez

Software Version 2.09.1

Experiment File Path: C:\Users\Public\Documents\Experiments\Practica_G7.xpt

Blank Read 2:412

PARA LA PRUEBA peso inicial uL peso final (mg/mL)

PARA REACTIVOS BUFFER C 15000 PREPARAR 10 mL

Ajustar a pH8 con HCl 2M (140uL) y ajustar volumen a

Concentraciones iniciales inhibibdor Donepezilo:

R-Squared [Blank Read 2:412]

t at Max V [Blank Read 2:412]

Lagtime [Blank Read 2:412]

15.2 18.633 Max V [Blank Read 2:412]

0:06:45 0:02:45 t at Max V [Blank Read 2:412]

0:00:55 0:00:37 Lagtime [Blank Read 2:412]

Max V [Blank Read 2:412]

R-Squared [Blank Read 2:412]