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Primer-BLAST» JOB ID:2tAFWOPh7knJd_Ry-RLQQIMJwXKuGtpvrw

Primer-BLAST Results

Input PCR template NC_011521.1 Neisseria gonorrhoeae plasmid pCmGFP, complete sequence
Range 4419 - 5135
Specificity of primers primers may not be specific to the input PCR template as targets were found in selected database:Custom (Organism limited
to Neisseria gonorrhoeae)...help on specific primers
Other reports Search Summary

Graphical view of primer pairs

NC_011521.1 Find: Tools Tracks
Template

Genes

(U) Primer pairs for job 2tAFWOPh7knJd_Ry-RLQQIMJwXKuGtpvrw

NC_011521.1: 4.3K..5.2K (931bp) Tracks shown: 3/7

Detailed primer reports

Primer pair 1
Template Self Self 3'
Sequence (5'->3') Length Start Stop Tm GC%
strand complementarity complementarity
Forward
GGCGATGGCCCTGTCCTTTT Plus 20 4983 5002 63.12 60.00 6.00 0.00
primer
Reverse
GCCATGTGTAATCCCAGCAGC Minus 21 5114 5094 61.95 57.14 4.00 3.00
primer
Product length 132

Products on potentially unintended templates

>Subject_1 Neisseria gonorrhoeae plasmid pCmGFP, complete sequence

product length = 132

Forward primer 1 GGCGATGGCCCTGTCCTTTT 20
Template 565 .................... 584

Reverse primer 1 GCCATGTGTAATCCCAGCAGC 21

Template 696 ..................... 676

Primer pair 2
Template Self Self 3'
Sequence (5'->3') Length Start Stop Tm GC%
strand complementarity complementarity
Forward
AGAGTGCCATGCCTGAAGGT Plus 20 4672 4691 61.50 55.00 4.00 2.00
primer
Reverse
AGGACAGGGCCATCGCCAAT Minus 20 4999 4980 64.13 60.00 6.00 3.00
primer
Product length 328

Products on potentially unintended templates

>Subject_1 Neisseria gonorrhoeae plasmid pCmGFP, complete sequence

product length = 328

Forward primer 1 AGAGTGCCATGCCTGAAGGT 20
Template 254 .................... 273

Reverse primer 1 AGGACAGGGCCATCGCCAAT 20

Template 581 .................... 562

Primer pair 3
Template Self Self 3'
Sequence (5'->3') Length Start Stop Tm GC%
strand complementarity complementarity
Forward
TCCAATTGGCGATGGCCCTG Plus 20 4976 4995 63.19 60.00 8.00 2.00
primer
Reverse
TGCCATGTGTAATCCCAGCAGC Minus 22 5115 5094 63.48 54.55 4.00 3.00
primer
Product length 140

Products on potentially unintended templates

>Subject_1 Neisseria gonorrhoeae plasmid pCmGFP, complete sequence

product length = 140

Forward primer 1 TCCAATTGGCGATGGCCCTG 20
Template 558 .................... 577

Reverse primer 1 TGCCATGTGTAATCCCAGCAGC 22

Template 697 ...................... 676

Primer pair 4
Template Self Self 3'
Sequence (5'->3') Length Start Stop Tm GC%
strand complementarity complementarity
Forward
ACCTGTTCCATGGCCAACACT Plus 21 4577 4597 62.23 52.38 6.00 1.00
primer
Reverse
GGACAGGGCCATCGCCAAT Minus 19 4998 4980 62.72 63.16 6.00 3.00
primer
Product length 422

Products on potentially unintended templates

>Subject_1 Neisseria gonorrhoeae plasmid pCmGFP, complete sequence

product length = 422

Forward primer 1 ACCTGTTCCATGGCCAACACT 21
Template 159 ..................... 179

Reverse primer 1 GGACAGGGCCATCGCCAAT 19

Template 580 ................... 562

Primer pair 5
Template Self Self 3'
Sequence (5'->3') Length Start Stop Tm GC%
strand complementarity complementarity
Forward
AGAGTGCCATGCCTGAAGGTT Plus 21 4672 4692 62.01 52.38 4.00 2.00
primer
Reverse
AAGGACAGGGCCATCGCCAA Minus 20 5000 4981 64.33 60.00 6.00 2.00
primer
Product length 329

Products on potentially unintended templates

>Subject_1 Neisseria gonorrhoeae plasmid pCmGFP, complete sequence

product length = 329

Forward primer 1 AGAGTGCCATGCCTGAAGGTT 21
Template 254 ..................... 274

Reverse primer 1 AAGGACAGGGCCATCGCCAA 20

Template 582 .................... 563

Primer pair 6
Template Self Self 3'
Sequence (5'->3') Length Start Stop Tm GC%
strand complementarity complementarity
Forward
AAGAGTGCCATGCCTGAAGGT Plus 21 4671 4691 62.01 52.38 4.00 2.00
primer
Reverse
GGGCAGATTGTGTGGACAGGT Minus 21 5037 5017 62.61 57.14 4.00 2.00
primer
Product length 367

Products on potentially unintended templates

>Subject_1 Neisseria gonorrhoeae plasmid pCmGFP, complete sequence

product length = 367

Forward primer 1 AAGAGTGCCATGCCTGAAGGT 21
Template 253 ..................... 273

Reverse primer 1 GGGCAGATTGTGTGGACAGGT 21

Template 619 ..................... 599

Primer pair 7
Template Self Self 3'
Sequence (5'->3') Length Start Stop Tm GC%
strand complementarity complementarity
Forward
CCAATTGGCGATGGCCCTGT Plus 20 4977 4996 63.48 60.00 8.00 2.00
primer
Reverse
CCATGCCATGTGTAATCCCAGC Minus 22 5118 5097 61.91 54.55 7.00 2.00
primer
Product length 142

Products on potentially unintended templates

>Subject_1 Neisseria gonorrhoeae plasmid pCmGFP, complete sequence

product length = 142

Forward primer 1 CCAATTGGCGATGGCCCTGT 20
Template 559 .................... 578

Reverse primer 1 CCATGCCATGTGTAATCCCAGC 22

Template 700 ...................... 679

Primer pair 8
Template Self Self 3'
Sequence (5'->3') Length Start Stop Tm GC%
strand complementarity complementarity
Forward
GTGGAGAGGGTGAAGGTGATGC Plus 22 4507 4528 62.89 59.09 2.00 2.00
primer
Reverse
ACCTTCAGGCATGGCACTCT Minus 20 4691 4672 61.50 55.00 4.00 0.00
primer
Product
185
length

Products on potentially unintended templates

>Subject_1 Neisseria gonorrhoeae plasmid pCmGFP, complete sequence

product length = 185

Forward primer 1 GTGGAGAGGGTGAAGGTGATGC 22
Template 89 ...................... 110

Reverse primer 1 ACCTTCAGGCATGGCACTCT 20

Template 273 .................... 254

Primer pair 9
Template Self Self 3'
Sequence (5'->3') Length Start Stop Tm GC%
strand complementarity complementarity
Forward
CAAGAGTGCCATGCCTGAAGGT Plus 22 4670 4691 63.11 54.55 4.00 2.00
primer
Reverse
AGGGCAGATTGTGTGGACAGG Minus 21 5038 5018 62.34 57.14 4.00 0.00
primer
Product
369
length

Products on potentially unintended templates

>Subject_1 Neisseria gonorrhoeae plasmid pCmGFP, complete sequence

product length = 369

Forward primer 1 CAAGAGTGCCATGCCTGAAGGT 22
Template 252 ...................... 273

Reverse primer 1 AGGGCAGATTGTGTGGACAGG 21

Template 620 ..................... 600

Primer pair 10
Template Self Self 3'
Sequence (5'->3') Length Start Stop Tm GC%
strand complementarity complementarity
Forward
TGGAGAGGGTGAAGGTGATGC Plus 21 4508 4528 61.79 57.14 2.00 2.00
primer
Reverse
AGTGTTGGCCATGGAACAGGT Minus 21 4597 4577 62.23 52.38 6.00 2.00
primer
Product length 90

Products on potentially unintended templates

>Subject_1 Neisseria gonorrhoeae plasmid pCmGFP, complete sequence

product length = 90
Forward primer 1 TGGAGAGGGTGAAGGTGATGC 21
Template 90 ..................... 110

Reverse primer 1 AGTGTTGGCCATGGAACAGGT 21

Template 179 ..................... 159

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