Documentos de Académico
Documentos de Profesional
Documentos de Cultura
Supervisor
Dr.
Elmer
Ccopa
Rivera
/
PAT-CTBE
Co-Supervisor
Celina
Kiyomi
Yamakawa
/
PIN-CTBE
Supervisor
Dr. Elmer Ccopa Rivera / PAT-CTBE
Co-Supervisor
Celina Kiyomi Yamakawa / PIN-CTBE
Campinas, SP - 2013
Agradecimientos
A mis padres, Myriam Garca Sierra y Norberto M. Herrera Tapia, de quienes he
recibido todo el amor, el apoyo y la disciplina. Ellos son los cimientos de todos mis proyectos
realizados y los que pretendo realizar.
A mi hermano, Norbertt Herrera Garca, por ser autntico, apoyarme y desearme lo mejor
siempre, a pesar de los desacuerdos.
A mi familia, por celebrar a lo grande cada uno de mis logros y acompaarme muy de cerca en
los estresantes das de espera; abuelos, tos y primos sin los cuales no habra tenido tanta
confianza, especialmente a Michelle, que vivi todo el proceso conmigo y sin quien no habra
sido posible.
A Mario, por esa confianza ciega desde el inicio. Por ser siempre la voz del realismo,
por su apoyo an desde lejos y sobre todo, por estos 5 maravillosos aos juntos.
A Mara y Karen, por seguir aqu sin importar el paso de los aos, celebrando nuestros
logros.
A mis amigos Rolando, Myriam y Risela, por los nimos y el apoyo todos los das, hacen mi
vida cotidiana mucho ms fcil y entretenida.
A mis profesores, el Dr. M. A. Salgado Cervantes y la Dra. Dolores Reyes Duarte, su
apoyo fue fundamental en la realizacin de esta experiencia. Especiales agradecimientos al
Profesor Alejandro Gonzlez Valdz por presentarme esta gran oportunidad e impulsarme a
aprovecharla, por su apoyo y comprensin durante todo el proceso.
A mi asesor Elmer A. Ccopa Rivera y co-asesora Celina Kiyomi Yamakawa, por todas
sus enseanzas, dedicacin y tiempo durante mi estancia en CTBE, gracias por la confianza
depositada; fue para m una experiencia muy grata el haber podido trabajar con ustedes.
A Fernanda Keile Gabrielli por toda la paciencia y su apoyo en mis primeros das.
A Victor Coelho, Lucas Pavanello y Sayonara Soares, por su amistad y compaerismo todos
los das.
A mis colegas Becarios, por hacer de esta una experiencia de vida completa, pero en
especial a Thiago (Alberto), Fi, Paola (mostri), Dulcinea, Patricio (Pato) e Izabel, por haber
sido mi familia durante 2 meses, por las risas y noches de desvelo compartidas, por su empata
en momentos de crisis y porque son lazos que conservar toda la vida.
A Tatiane Madruga Morais y Roberto Pereira Medeiros por su hospitalidad y cuidados
durante todo el proceso.
Por ltimo agradezco al CNPEM por la gran oportunidad que me fue brindada a travs del 22
Programa de Becas de Verano para desarrollarme en el campo de la investigacin en un centro
tan importante como lo es el CTBE.
Resumo
A configurao de plantas de fermentao industrial no Brasil
predominantemente em batelada alimentada com reciclo de clulas. Neste modo de
operao, a levedura exposta a inibidores atravs de longos perodos de tempo e a
concentraes elevadas de clulas, bem como a flutuaes na qualidade da matriaprima, com impacto na cintica do processo e no desempenho operacional. Neste
contexto, para a implementao de estratgias de operao adequadas, necessrio
dispor de modelos cinticos capazes de descrever o processo em batelada alimentada, o
mais realista possvel. Assim, neste trabalho, avaliou-se um processo de fermentao
em batelada alimentada com reciclo de clulas, utilizando levedura industrial e caldo de
cana como substrato..
A preciso da previso de um modelo cintico avaliada no apenas por sua
preciso na descrio de observaes experimentais, mas, essencialmente, pelos
desafios envolvidos na estimativa de seus parmetros. Fermentaes sucessivas de
experimentos em batelada alimentada foram realizadas para desenvolver o mtodo para
a estimativa de parmetros cinticos. O modelo foi capaz de prever com preciso as
fermentaes em batelada alimentada, aps a etapa de tratamento de levedura.
Observou-se medidas de desempenho aceitaveis (RSD e R2) para a previso das
concentraes de clulas, substrato e etanol.
Abstract
The configuration of industrial fermentation plants in Brazil is predominantly
fed-batch culture with cell recycle. In this mode of operation, yeast is exposed to
inhibitors through long time periods and under high cell concentrations as well as
fluctuations in the quality of the raw material, with impact on process kinetic and
operating performance.
In this context, for implementation of suitable operational strategies, it is
necessary to have fed-batch kinetic models able to describe the process as much realistic
as possible. Bearing this in mind, in this work the alcoholic fermentation by industrial
yeast strain and sugarcane juice in fed-batch with cell recycle was assessed. The
accuracy of prediction of a mechanistic kinetic model is evaluated not only by their
precision in describing experimental observations, but essentially by the challenges
involved in the estimation of their parameters. Fed-batch experiments in successive
fermentations were performed to develop the method for estimation of kinetic
parameters. The model was able to accurately predict the fed-batch fermentations after
the yeast treatment step. It was observed an acceptable performance measures (RSD and
R2) for prediction of cell, substrate and ethanol concentrations.
Summary
Resumo/Abstract ......................................................................................................................... 2
1.
Introduction ......................................................................................................................... 4
Objectives ........................................................................................................................... 11
Methodology ....................................................................................................................... 12
6. Conclusions............................................................................................................................24
7.References...............................................................................................................................25
1. Introduction
For the last few years, the alternative energy field has been expanding in order to
compensate the fuel demand worldwide dealing with fossil fuels problems such as
unavailability of resources and high greenhouse emissions.
Nowadays, ethanol has been established as the best fuel alternative and a fair
competition to gasoline, replacing approximately 50% of the gasoline that would be
used in Brazil if there wasnt another option (Goldemberg, 2013).
The cultivation of sugarcane in Brazil is one of the most notorious worldwide,
this makes it a highly available resource for exploitation.
The total use of sugarcane (bagasse and straw included) as raw cellulosic
material has become an important alternative for ethanol industrial production processes
by depolymerizing, through hydrolysis, the cellulose and hemicelluloses fractions into
fermentable sugars (Andrade, 2013).
In Brazil, ethanol has been produced by industrial fermentation processes since
the 20th century. Currently there are 432 mills and distilleries processing about 625
million tons of sugarcane per crop, resulting in a production about 27 billion liters of
ethanol and 38.7 tons of sugar (Amorim and Lopes, 2011).
Even though Brazil is a pioneer and a leader in fermentative processes for
biofuels production, there are still a lot of problems that need to be taken care of, such
as the cost of production, the complete exploitation of sugarcane and the yeast cell
recycling, which is not yet explored as much.
The main purpose of this work is to generate a mathematical model that can
adjust and predict the effects, advantages and disadvantages of cell recycling in fedbatch fermentation for ethanol industrial production processes.
It is also intended that it can be applied to the processes already being used in
order to improve the efficiency and lower the costs.
1.1 Background
Due to the fossil energy depletion and the need to reduce green house gas
emissions and their effects on global warming, alternative energy sources must be
developed. Biofuels, derived from renewable resources are realistic and viable
substitutes to fossil fuels.
At the present time, bioethanol, the main biofuel produced by fermentation of
several feedstocks, constitutes a rapid and significant answer to these problems which is
already being taken into account (Amillastre et al., 2012).
Among all forms of producing ethanol, the fermentation route is the most
economically profitable to Brazil. This fact is due mainly to Brazilian geographic
location, type of soil, variety of feedstock and possibility of nationwide cultivation
(Basso et al., 2011).
Brazil is one of the largest producers of sugarcane worldwide and responsible of
sugar accounting for approximately a quarter of the entire worlds production
(Goldemberg, 2013). Thus, Brazil is the most competitive producer of bioethanol from
sugarcane in the world with a well developed domestic market thats also being
increasingly stimulated by growing sales of flex fuel cars.
At the beginning the priority was to produce anhydrous bioethanol so that it
could be mixed with gasoline; after the world oil crisis that took place in the late 70s
this objective turned into the initiation of ethanol-powered vehicles. This plan was
successful and it culminated in an increase of vehicles that functioned on hydrated
ethanol in Brazil to the point where the occupation of these cars occurred in almost
100% of the country.
In 2008 bioethanol consumption as a fuel exceeded the consumption of gasoline
in Brazil; currently more than 95% of all the cars sold in Brazil are flex-fuel (meaning
they can run either on ethanol or gasoline).
In Brazilian ethanol production industry, the fermentation is a biochemical
process in which glucose, fructose and sucrose (from sugarcane juice and sugarcane
molasses in varying proportion) is metabolized to ethanol by yeast Saccharomyces
cerevisiae in fermentors containing millions of liters. Ethanol yields in the order of 90
92% of the theoretical sugar conversion into ethanol were achieved in the last decade
(Della-Bianca et al., 2012).
Gasoline sold in Brazil nowadays contains 25% anhydrous bioethanol. The
expansion of ethanol consumption due to the growing fleet of light vehicles, mainly flex
fuel cars, and increased exports have opened new opportunities for industrial growth.
However, the success of these industries depends on how they solve and face the
challenges that this new fuel brings to the table.
1.2 First and Second Generation Ethanol
At present, there are two main streams in biofuels production: First and Second
generation biofuels (see Figure 1).
First generation biofuels
First generation or conventional biofuels are the ones produced from raw
materials ready for fermentation; these raw materials dont mandatorily need a
pretreatment. Some examples of these feedstocks are mainly crops rich in starch such as
grains, sugarcane and corn.
Starch is a glucose-containing polymer which can readily be hydrolyzed by
industrially produced enzymes and fermented by yeast strains. This process is well
known and is being currently applied for industrial production of bioethanol in many
parts of the world, especially in the USA (whit corn as feedstock) and Brazil (with
sugarcane as feedstock).
Second Generation Biofuels.
Second-generation biofuels are the ones produced from sustainable feedstock.
These feedstocks are usually lignocellulosic materials which need to be pre-treated for
the fermentation (hydrolysis or thermo-chemical pretreatments). After this additional
step, the sugar released during the pretreatment is fermentation ready to produce
ethanol. Wood, bagasse and straw are the most common lignocellulosic resources for
this type of fermentation.
Figure 1: Flowchart for first and second generation ethanol production processes (Tan et al., 2008)
by predicting the behavior of the fermentation for it to perform in a more efficient way
and thus reduce the costs and time of production, as well as determinate the optimal
conditions for the process to work at its highest levels of performance.
In this work an alcoholic fermentation by an industrial yeast strain
(Saccharomyces cerevisiae from LEB-UNICAMP) and sugarcane juice in fed-batch
with cell-recycle was performed. These experiments were assessed in order to study and
analyze the kinetic model with focus on a method that may be used always a reestimation of parameters is required. In this sense, the performance of a mechanistic
kinetic model, considering the effect of cell recycling on the kinetic, is evaluated not
only by their accuracy in describing experimental data, but mainly by the difficulties
involved in the adaptation of their parameters. Fed-batch experiments with cell recycle
were performed to develop the method for estimation of kinetic parameters.
1.4 The Melle-Boinot process
The actual fermentation process was developed in the 1930s by Firmino Boinot and
this technology was patented in 1937. Melle-Boinot Process is the most popular
fermentation technology being used in Brazil; this process involves yeast recovery for
cell recycling by wine centrifuging.
Yeast cell recycling represents an advantage for the industry because the reutilization of the living cell biomass saves sugar and increases the fermentation yield
because, according to Amorim and Lopez (2005), instead of the yeast converting sugar
into the cellular biomass, more sugar is converted into ethanol. For this reason, other
processes worldwide without cell recycle cannot compete with Brazilian distilleries
ethanol yields (Amorim et al., 2011).
Typical configuration of a Melle-Boinot fermentation process is presented in
Table 1.
Table 1: Data table of Melle-Boinot process traditional configuration (Johnson and Seebaluck, 2012)
In the last 30 years this process has been improved allowing Brazilian distilleries
to achieve yields up to 92-93%.This yield refers specifically to ethanol produced from
sugar, nonetheless there are various sub-products such as glycerol, cellular biomass,
succinate and malate (Amorim et al., 2011); now every industrial fermentation plant in
Brazil uses modified Melle-Boinot processes to produce ethanol on an industrial scale.
Disadvantages of the Melle-Boinot process
Despite the high level yields that this process achieves, it presents a few
significant problems such as contamination risks and loss of activity because of cell
recycling and stressful conditions.
In fermentation process with cell recycling the yeast cells are being constantly
submitted to stressful conditions such as high ethanol levels, low pH, temperature,
excess of salts and mineral deficiency among others; due to this the first challenge
nowadays for process improvements is the comprehension of how this parameters affect
the yeast cells and the fermentation. Although several laboratories are now working in
the reproduction of these conditions at bench scale, its still very difficult to understand
how the yeast is being affected at an industrial scale since the results of other
fermentation processes cant be applied to the Brazilian distilleries because of the
difference in the conditions between sugar cane and other feedstocks such as wheat,
corn, etc (Amorim et al, 2011).
10
2. Objectives
2.1 General Objective:
The main purpose of this work is to evaluate the prediction accuracy of a
mechanistic kinetic model not only by its precision in describing experimental
observations, but essentially by the challenges involved in the estimation of their
parameters.
2.2 Specific Objectives:
1. Development of experiments for a fed-batch fermentation process with cell recycles
using industrial yeast strain and sugarcane juice.
2. Development and testing of a modeling approach for the kinetic parameter
estimation with focus on a method that may be used when a re-estimation or
comparison of parameters is required.
2.1 Evaluate optimization criteria expressions to find optimal values for the
parameters that result in the closest fit between the experimental
observations and the simulated response variables.
2.2 Evaluate the performance of the model considering the effect of cell
recycling on the kinetic.
11
3. Methodology
3.1 Fed Batch Fermentation: Experimental Part
For the first part of this study a fed-batch fermentation experiment was assessed
to produce the appropriate conditions to emulate the industrial conditions of the
Brazilian industrial alcoholic fermentation.
The characteristics of this experiment are presented in Figure 2.
Glucose Solution
PA
500g/L:
1800.00
mL
Water:
7200.00
mL
Must
12
Previous the inoculum preparation, three slopes from agar plate were transferred
to liquid complex medium containing per liter of desmineralized water: yeast extract, 10
g; peptone, 20 g; and glucose, 20 g. This step named pre inoculum aimed cells
activation that was performed in flask shaker culture for 24 hours at 33C and 250 rpm.
Inoculum and cultivation
The complex medium used for inoculum and cultivation contained the following
per liter of desmineralized water: K2SO4, 6.6 g; KH2PO4, 3 g; MgSO4, 0.5 g;
CaCl2.2H2O, 1.0; and yeast extract, 5.0 g. After autoclaving at 121C for 15 minutes, the
medium was cooled to room temperature. Thereafter, filter-sterilized elements were
added in the following concentration per liter: urea, 2.3 g; thiamine, 3.0 g; EDTA, 15
mg; ZnSO4.7H2O, 4.5 mg; CoCl2.6H2O, 0.3 mg; MnCl2.4H2O, 0.84 mg; CuSo4.5H2O,
0.3; FeSO4.7H2O, 3 mg; NaMoO4.2H2O, 0.4 mg; H3BO3, 1 mg; and KI, 0.1 mg. The
carbon source, 80 g/ L of glucose, was sterilized separately at 121C for 15 minutes.
The inoculum culture was performed in Erlenmeyer flask for 24 hours, 33C and
250 rpm in an orbital shaker incubator (Innova 44 New Brunswick). After that the
inoculum was centrifuged in a Sorvall centrifuge at 8000 rpm for 20 minutes, then the
supernatant was discarded and the cells were suspended in sterilized water up to 200 mL
and transferred to the cultivation bioreactor aseptically. The cultivation was performed
at 33C in a bioreactor (Bioflo 115; New Brunswick Scientific) (as shown in Figure 4)
in fed batch configuration in cascade control with agitation and air flow to maintain the
dissolved) O2 concentration above 60% of saturation with air. Thereafter, the yeast
culture was centrifuged in a Sorvall centrifuge at 8000 rpm for 20 minutes, then the
supernatant was discarded and the cells were suspended in sterilized water (quantity
sufficient for 800 mL) and transferred to the bioreactor for alcoholic fermentations.
13
The substrate used for alcoholic fermentations was slight the same of cultivation but
glucose concentration was per liter of desmineralized water 198.53 g in the first recycle,
197.78 g in the second recycle and 136.44 g in the third recycle. The alcoholic
fermentations were performed with cells recycling in the fed batch configuration as is
usual in industrial Brazilian process (Melle-Boinot). However the cell density was not
high as at industrial process (approximately 30 g/ L in the end of must feed) whereas the
main aim in these experiments was to obtain many data to obtain kinetic tendency in fed
batch.
The yeast cells required were obtained previously in the cultivation step. The batch
feeding using must was performed in nine hours (flow of 2.26 mL/min) up to the final
volume of 2 L. After that, the wine was then centrifuged meaning not ensure that all
sugar was consumed. The fermented wine was centrifuged at 8000 rpm for 20 minutes
in a Sorvall centrifuge then the yeast was suspended with sterilized water and
centrifuged again in the same condition above. The centrifuged yeast biomass was
carried back to the bioreactor for treatment with H2SO4 under pH of 3.0 and aeration
during one hour. This treatment was performed before each fermentative cycle during
yeast cell recycling in other words fermentation and yeast recover step and recycle were
carried out for four cycles (see figure 5).
Samples were taken on every hour of each fermentation cycle in triplicates, for them
to be analyzed later and determinate the products and the yields of the fermentation and
14
discuss how does the cell recycling affects the yeast cells and impact on the
fermentation kinetics.
De-yeasted
wine
1st.
Recovery
Cells
H2SO4
pH=3
T=30
min.
150
rpm
0.1
vvm
Detoxification
2nd Recovery
Yeast
Treatment
Reinvigoration
must
Fermentation
15
4. Results
The first part of this study includes data obtained from 5 fed-batch fermentations (4
cell recycles) in which the triplicate samples of each fermentation cycles were weight to
determine the dry mass data contained in 2 mL eppendorfs: MA (empty eppendorfs
weight), MD (eppendorfs weight with dry cell weight) and MC (dry cell weight).
These data were analyzed in order to obtain concentration values for the samples.
As it was previously mentioned, the samples were taken in triplicates and to avoid any
probabilistic error, the standard deviation between the 3 samples was calculated. The
results of this analysis werent significant, meaning that the difference between the
samples of each time wasnt even enough to plot them.
After performing these analyses, the profiles of X (cell concentration, g/L) in
function of the time (hours) were plotted, as shown in Figure 6.
12.00
Concentration
X
(g/L)
-
all
values
8.00
12.00
Concentration X (g/L)
Concentration (g/L)
10.00
Concentration X (g/L)
14.00
6.00
4.00
2.00
Concentration X (g/L)
10.00
8.00
6.00
4.00
2.00
0.00
0.00
0
4 Time
5 (h) 6
-2
10
a)*
Concentration
X
(g/L)
6.00
4.00
2.00
0.00
4
Concentration X (g/L)
Concentration X (g/L)
8.00
10
Concentration X (g/L)
12.00
Concentration
X
(g/L)
10.00
b)
12.00
-2
Time (h)
Concentration X (g/L)
10.00
8.00
6.00
4.00
2.00
0.00
10
-2
Time (h)
10
Time (h)
d)*
c)
Figure 6: X against time for first (a), second (b), third (c) and forth (d) recycle.
(*
The fluctuations in concentration values can be considered as a result of a decalibration of the
analytical balance between samples.)
16
Figure 7 shows the concentration values obtained from the HPLC analysis for ethanol,
glucose, and cells concentrations (g/L):
120.00
90.00
80.00
100.00
70.00
80.00
60.00
50.00
Ethanol
40.00
Glucose
30.00
Cells
Ethanol
60.00
Glucose
40.00
20.00
Cells
20.00
10.00
0.00
0.00
0
10
a)
10
b)
120.00
120.00
100.00
100.00
80.00
80.00
Ethanol
60.00
40.00
Ethanol
60.00
Glucose
Glucose
40.00
Cells
20.00
Cells
20.00
0.00
0.00
10
c)
10
d)
Figure 7: Graphics that represent the concentration of Ethanol (), Glucose (), and Cells () in g/L
plotted against time (hours).
17
5. Mathematical
modeling
This section presents the considerations required to develop a model-based
technique for the estimate of the kinetic parameters.
5.1 Kinetic model
The state variables involved in this fed-batch process were concentration of total
cell mass, X (kg/m3), concentration of substrate, S (kg/m3) and concentration of ethanol,
P (kg/m3).
Experimental observations have shown that cell, substrate and product inhibitions
are significant for ethanol fermentation (Rivera et al., 2007; Andrade et al., 2013). Eq
(1) shows the cell growth rate equation, rx, which includes terms for such types of
inhibitions:
rx = max
S
X
exp(K iS)1
Ks + S
X max
P
1
Pmax
(1)
where max is the maximum specific growth rate (h1), Ks the substrate saturation
constant (kg/m3), Ki the substrate inhibition parameter (m3/kg), Xmax the cell
concentration where the growth ceases (kg/m3), Pmax the ethanol concentration where
the cell growth ceases (kg/m3), and m and n are empirical parameters.
In this study, a modified Luedking-Piret expression was used to account for the ethanol
formation rate, rp, as shown in Eq (2). This rate depended on the specific growth rate
and cell concentration (X). Yp/x (kg/kg) is the product yield based on cell growth, m
(kg/kg h) is a parameter associated with maintenance, and Ks (kg/m3) a saturation
parameter.
rp = p/x rx +
mS
X
K s + S
(2)
rs = (rx / x ) + ms X
(3)
Were Yx (kg/kg) and ms (kg/kg h) denote the limit cellular yield and maintenance
parameter.
18
F X
dX
= rx A
dt
V
(4)
- Substrate
dS FA (SA S )
=
rs
dt
V
(5)
- Ethanol
F P
dP
= rp A
dt
V
(6)
- Volume
dV
= FA
dt
(7)
The mass balance differential equations were solved with the using the
Livermore Solver for Ordinary Differential Equations (LSODE, Radhakrishnan and
Hindmarsh 1993).
Initial dataset
Fed-batch model
State
variables
(Xn,
Sn,
Pn)
Stop?
Optimization step
Minimize
cost
function:
E()
Experimental
data
(Xen,Sen,Pen)
End
Figure 8: General framework of the model-based approach used to estimate the kinetic parameters
19
E () =
(X n Xe n ) 2
Xe 2max
n =1
np
E() =
(X n Xe n ) 2
n =1 X n
Xe n
(S n Se n ) 2
Se 2max
(S n Se n ) 2
S n Se n
(Pn Pe n ) 2
(8)
Pe 2max
(Pn Pe n ) 2
Pn Pe n
(9)
20
Fermentation
2
Fermentation
3
Fermentation
4
17.95
9.84
40.55
3.37
14.55
44.15
15.52
14.56
44.83
4.22
11.13
44.65
0.5
171.7
0.43
2
0.5
171.7
0.26
3
0.5
171.7
0.22
3
0.5
171.7
0.23
3
The parameter estimation process requires appropriate initial guess values to start
the optimization process. This step is critical to find the optimal values of the
parameters that minimize the error between the experimental and simulation data.
Fortran routines was used to accomplish this procedure. Two cost functions were
compared (Equations 8 and 9) and the results are show in Table 3.
The parameters shaded in green were the only ones varied during the adjustment.
In this study, mp, m, Ks also were studied. The remaining ones were fixed in the
previous values used in several studies (Atala et al., 2001; Rivera et al., 2007, Andrade
et al., 2013 ), as follows: Ks = 4.1 (kg/m3), Ki = 0.004 m3/kg, m = 1.0 and n = 1.5.
21
Description
Ferm. 1
Ferm. 2
Ferm. 3
Ferm. 4
Obj. Eqn. 1 Obj. Eqn. 2 Obj. Eqn. 1 Obj. Eqn. 2 Obj. Eqn. 1 Obj. Eqn. 2 Obj. Eqn. 1 Obj. Eqn. 2
4.1
4.1
4.1
4.1
4.1
4.1
4.1
4.1
0.02
0.02
0.02
0.02
0.02
0.02
0.02
0.02
Pmax (g/L) Product concentration whe cell growth ceases. 75.61201899 92.7767951
72.459508
98.176
n
m
1.5
1
1.5
1
1.5
1
1.5
1
1.5
1
1.5
1
1.5
1
1.5
1
9.6993
10.23472122
5.5924
0.3057
0.365345316
0.3484
0.85
0.85
0.84
0.84
0.795
0.795
0.917
0.917
0.482
0.482
0.55
0.55
12.75
12.75
13.65
13.65
5.96
5.96
5.84
5.84
1.1328
a)
d)
b)
e)
c)
f)
)
Figure 9: Cell (X), substrate (S) and ethanol (P) concentrations plotted against time, being the dotted
line (---) the plot corresponding to equation (8) and the continuous line corresponding to equation (9).
a), b), and c) correspond to Recycle 1; d), e) and f) correspond to recycle 2.
22
g)
j)
h)
k)
i)
l)
Figure 10: cell (X), substrate (S) and ethanol (P) concentrations plotted against time, being the dotted
line (---) the plot corresponding to equation (8) and the continuous line corresponding to equation (9).
g), h), and i) correspond to Recycle 3; j), k) and l) correspond to recycle 4.
Table 4: Statistical Criteria to characterize the prediction quality of the fed batch model:
Objective equation 1.
S
P
Obejctive equation 2.
S
P
RECYCLE 1
RSD(%)
2
R
5.03
12.22
3.02
5.96
8.35
6.35
0.97
1.00
1.00
0.99
1.00
1.00
RECYCLE 2
RSD(%)
2
R
11.56
22.96
6.52
7.94
23.98
4.99
0.98
0.98
1.00
0.98
0.98
1.00
RECYCLE 3
RSD(%)
2
R
6.37
7.83
7.82
4.81
10.42
9.36
0.99
0.99
0.99
0.98
0.99
0.98
RECYCLE 4
RSD(%)
2
R
7.20
4.90
7.12
6.21
6.00
7.19
1.00
1.00
1.00
1.00
0.99
1.00
23
6. Conclusions
This work presents results from the development and testing of a modeling approach
for the estimation parameters in fed-batch fermentation with high cell densities
intensively recycled. Even considering that the kinetic rate expressions are known in the
mechanistic model, the estimation problem is complex and time consuming.
This suggests that using a model in a situation where frequent parameter reestimation is necessary, such as the studied system could be a limitation. In this work, a
model-based approach has been developed using a mechanistic model and optimization
algorithms that have been widely used for modeling and optimization purposes in
engineering application.
Based on this approach, a mechanistic model was obtained and its performance in
describing the dynamic behavior of cell, substrate and ethanol concentrations during
fed-batch fermentation was assessed. Model predictions using the experimental
observations provided acceptable performance measures (RSD and R2). Finally, it can
be said that the use of this approach enables a rapid determination of a mathematical
description of fed-batch fermentation processes that can be used for optimization and
control.
24
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