Acetilcolinestera

sa
Damin Madrigal Aguilar
 Sustrato e inhibidores

Acetilcolinesterasa

SUSTRATO INHIBIDORES
Edrofonio, Carbamatos
Acetilcolina (Neostigmina), Organofosfatos
Colina+ Acetato (Malatin, Paratin)
 Localizacin

Clulas piramidales
SNC Corteza motora

Acetilcolina
Ncotinicos Simptica
Muscarnicos Preganglionares
SNP
Corazn
Estmago
Parasimptico Intestino
Preganglionares vegija
Motoneuronas Posganglionares
Msculo esqueltico
Va de sealizacin
 Estructura
Acetilcolinesterasa
Mecanismo de reaccin
V=Vmax [s]/Km+[s]

[S] Sin [I]=10 [I]=25 [I]=40

(M) [I]=0 M M M

1 9,09 3,23 1,69 0,85

2 16,67 6,25 3,23 1,60

4 28,57 11,77 6,25 2,60

6 37,50 16,67 9,09 4,90

8 44,44 21,05 11,77 5,60

10 50,00 25,00 14,29 8,00

20 66,67 40,00 25,00 16,00

30 75,00 50,00 33,33 20,00

40 80,00 57,14 40,00 25,00

50 83,33 62,50 45,46 33,00

 Y= mx+b
1/V=(Km/Vmax)(1/[S]) + 1/Vmax
 Y= mx+b
1/V=(Km/Vmax)(1/[S]) + 1/Vmax
1/[S]
1)Regresin lineal (
M
Sin
[I]
[I]=0.00
001
[I]=0.00
002
[I]=0.00
004

obtencin de m y b 1000
) =0 M 5M 0M

0
2) 1/Vmax=b , 0
0 110011 309598 591716 1176471

Vmax=1/b 5000
0
0 59988 160000 309598 625000
3)Km/Vmax=m 2500
0

Km=m Vmax 0
1666
35002 84962 160000 384615

6
7 26667 59988 110011 204082
1250
0
0 22502 47506 84962 178571
1000
0
0 20000 40000 69979 125000
5000
0 14999 25000 40000 62500
3333
 Y= mx+b
1/V=(Km/Vmax)(1/[S]) + 1/Vmax
Concentracin m b
inhibidor (Km/Vmax) (1/Vmax)

0M 0.100 9998

0.00001 M 0.299 10038

0.000025 M 0.583 11905

0.000040 M 1.177 20275

 Y= mx+b
1/V=(Km/Vmax)(1/[S]) + 1/Vmax
Concentraci Km (M) Km (M) Vmax
n
de inhibidor
0M 0.00001 0.0001
0.00001 M ***** 0.000029 0.0001
79
0.000025 M ***** 0.000048 0.000084
97
0.000040 M ***** 0.000058 0.000049
05
 Energa Libre de Gibbs
G=R T LnKm
R (8.314 J/K mol) T 310.15 K
concentracin G (Joules/mol)

0M -27687.08

0.00001 M -26872.32

0.000025 M -25590.67

0.000040 M -25152.06
log((km/km)-1)=log (I)-logKb

Log ((Km /
Log [I]" Km)-1)

5 0.29624501

4.602059991 0.590632619

4.397940009 0.681604604
log (V/(Vmax-1))=nlogS-logK