Concentración EI % v/v Concentracion DE ( EI) % v/v Conc DE ( EtOH) % v/v

2 0.2 1.99

Conc. Rel DE
0.995
Disolucion estandar
Area EtOH Area EI Area relativa C rel Fr Fr Promedio
87153 86290 1.01000116 0.995 1.01508 1.02512859
81210 78844 1.03000862 0.995 1.03518
86500 84804 1.01999906 0.995 1.02512

Muestra
Area EtOH Area EI Area RelativaConc. rel C% % v/v
20418 85190 0.23967602 0.23380093 0.04676 0.117
24512 102133 0.24000078 0.23411773 0.04682 0.117
19820 82928 0.23900251 0.23314393 0.04663 0.117
Curva de calibracion relativa

Datos

% EI acetona % DP etanol
10 10

Preparación de la curva de calibración

Resultados
Punto DP mL EI mL Vol final mL Conc DP % v/v
1 0.1 1 10 0.1 Punto
2 0.5 1 10 0.5 1
3 1 1 10 1 2
4 1.5 1 10 1.5 3
5 2 1 10 2 4
5

muestra de licor
Inyeccion
1
2
3
4
5

1.8
f(x
1.6
R²
1.4

1.2
A relativa

0.8

0.6

0.4

0.2

0
0
0.2

0
0
 AREAS EtOH AREAS EI Arel
Rep 1 Rep 2 Rep 3 rep 1 rep 2 rep 3 Rep1 Rep 2
30545 25864 30743 290080 249370 287220 0.10529854 0.10371737
109170 123050 139240 214770 246810 266110 0.50831122 0.49856165
228380 247660 255520 241720 236180 261540 0.94481218 1.04860699
321700 371000 387370 220850 254990 265390 1.45664478 1.45495902
481390 481950 433470 261410 261700 247950 1.84151333 1.84161253

muestra de licor
area EtOH area acetonaA rel con % v/v % v/v % v/v promedio
296800 187150 1.58589367 1.694 3.388 3.388
290840 185900 1.56449704 1.671 3.388
287960 185880 1.54917151 1.654 3.388
285800 183680 1.5559669 1.661 3.388
291010 187180 1.5547067 1.660 3.388

Curva de calibración
2

1.8
f(x) = 0.906277933213228 x + 0.0502058151521286
1.6
R² = 0.996098507827084
1.4

1.2
A relativa

0.8

0.6

0.4

0.2

0
0 0.5 1 1.5 2 2.5

% v/v EtOH
0.2

0
0 0.5 1 1.5 2 2.5

% v/v EtOH
Rep 3 promedio
0.10703642 0.10535077
0.52324227 0.51003838
0.97698249 0.99013389
1.45962546 1.45707642
1.74821537 1.81044708
Curva de calibración relativa

[ EI] mg / mL [ DP] mg/ mL

5 1

Datos de la curva de calibración

punto DP ( mL ) EI ( mL) Vol final mL conc DP mg /mL Area DP Area EI

1 0.02 0.01 10 0.002 19998 275556
2 0.04 0.01 10 0.004 39899 242563
3 0.06 0.01 10 0.006 61000 255448
4 0.08 0.01 10 0.008 81010 247076

20 10
40 10
60 10
80 10

Curva de calibración
0.35
0.3f(x) = 42.010579521052 x − 0.00911952918751
0.25R² = 0.99846011756738
Arearelativa

0.2
0.15
0.1
0.05
0
0.001 0.002 0.003 0.004 0.005 0.006 0.007 0.008 0.009
Diclorobenceno (mg /mL)
 Muestra
A rel Inyeccion Area Area EI Arel Con mg / mL ppm
0.07257327 1 65430 275556 0.2374472 0.00587003 5.87003161
0.16448923 2 65400 242563 0.26962068 0.00663605 6.63604857
0.23879615 3 65430 255448 0.25613824 0.00631505 6.31504573
0.32787482

.00911952918751

06 0.007 0.008 0.009

mL)
Conc ppm promedio
6.27370863473021
Por factor de respuesta

Pico Alcaloide Area 1 Area2 Area 3 A r1 Ar2 A rel 3

1 Esoartíena 690515 584481 587884 2.8285884 2.82246163 2.81163135
3 Lupanina 2948813 2527575 2516182 12.0793585 12.2056721 12.0339662
4 3- OH- lupanina 138221 123711 121251 0.56620105 0.59740103 0.57989861

Concentraciones mg / mL
Esparteina 0.1514
EI cafeina Lupanina 0.684
A1 A2 A3 3 OH 0.044
244120 207082 209090 Cafeina 0.1

Muestra
Pico Area 1 Area 2 Area 3 Arel 1 Arel 2 Arel 3
Esparteina 1 145848 175345 147997 0.66265328 0.71310342 0.62262619
Cafeina 2 220097 245890 237698 1 1 1
Lupanina 5 2191599 2573631 2263740 9.95742332 10.4665948 9.52359717
3- OH 7 147762 175634 159083 0.67134945 0.71427874 0.6692652
A rel promedio C rel Fr rel
2.8208937958742 1.514 1.86320594
12.10633229868 6.84 1.76993162
0.5811668954878 0.44 1.32083385

A rel promedio C rel C mg / mL C mg / 100 g Sumatoria

0.6661276314838 0.35751691 0.0358 17.875845513 325.808595
1 0
9.9825384354133 5.64007011 0.5640 282.00350533
0.6849644650199 0.51858488 0.0519 25.929243987
[DE]
[OCPB] mg / mL [OB] mg / mL Muestra % v/v
3.04 2.093 0.1

Disolucion estandar
Area OB Area OCPB Fr OB Fr OCPB
1650008 771567 788345.915 253804.9342105

Muestra
Area OB Area OCPB C OB mg / mLC OCPB mg / mL Cppm OB C ppm OCPB
532048 375730 0.67489155 1.48038887096 6748.91554 14803.8887
Normalizacion de areas

Nota:; Solo considerar el area de alcoholes

Pico Alcohol Area 1 Area 2 Area 3 Apromedio Area total A x / Atotal

3 Metanol 365134 350714 334699 350182.333 4755906.33 0.07363104
4 Etanol 3417384 3539710 3274528 3410540.67 0.71711687
5 Propanol 106710 109274 99385 105123 0.02210367
6 Isobutanol 168896 174293 157479 166889.333 0.03509096
8 Isoamilico 474225 494192 446721 471712.667 0.0991846
10 Hexanol 45866 48451 44430 46249 0.00972454
13 Nonanol 154264 163405 146662 154777 0.03254416
14 feniletanol 12231 13084 11726 12347 0.00259614
15 Bencilico 37519 38971 37766 38085.3333 0.00800801
%x Suma
7.36310408 100
71.7116871
2.21036733
3.5090963
9.91845999
0.97245397
3.25441649
0.25961403
0.80080074
Acido graso Conc mg / mLArea Fr
C 16 0.02 107682 5384100
C 18 0.02 97061 4853050
C 18 : 1 0.024 98597 4108208.33

Muestra Area Conc mg / mL Conc mg / g

C 16 171984 0.03194294311 0.21295295
C 18 145681 0.03001844201 0.20012295
C18 : 1 148440 0.03613253953 0.2408836
Curva de calibrción relativa

[EI] mg / mL [ DE] MP [ DE ] PP Muestra g [Muestra] ( g / mL)

1 0.2 0.1 2.0719 0.20719
.

Disolucion estadanr

Metil parabeno butilparabeno Arel Conc rel mg / mL Fr rel

62783 73883 0.84976246 0.2 4.24881231135715

Muestra
mparabeno bparabeno A rel Conc. rel Conc. mg/ mL Conc % m/ m
33205 73079 0.4543713 0.106940778627 0.106940778626825 0.00051615 0.052
Datos Datos de la muestra
[ EI] mg / mL[ DE C12] mg / mL [ DE C18:1] mg/ mL Vol muestra ( mL) Vol de aforo ( mL)
4 0.6 1.068 1 5

Datos del estandar

Pico Acido grasp Area Arel Conc rel Fr rel

1 C 12 167.04 1.09945369578095 0.15 7.32969131
2 C 14 151.93
3 C18:1 303.2 1.99565589416178 0.267 7.47436664

Datos de la muestra

Pico Acido grasp Area A rel Crel Con mg / mL

1 C 12 34.01 0.4110466521634 0.0560796676214 0.22431867
2 C 14 82.74
3 C18:1 53.27 0.64382402707276 0.08613760304636 0.34455041
% g/mL
1.122

1.723
Análisis de ácidos grasos

M16 Datos mg / mL dis DE mg/mL Conc. Relativas Area relativa Factor rel.
M18 [DP] M16 0.2 0.04 0.4 0.6213 1.5534
M18:1 [DP] M18 0.103 0.0206 0.206 0.3770 1.8302
M15 (EI) [DP] M18:1 0.306 0.0612 0.612 1.0091 1.6488
[EI] M15 0.50 0.1 1
Reporta en:
mg / g
Para la muestra

Area rel. Conc. Rel Conc. Mg / mL mg mg/g

M 16 0.804 0.517 0.052 0.2587 17.2457348
M18 0.105 0.057 0.006 0.0286 1.90672131
M18:1 0.986 0.598 0.060 0.2989 19.9270661
ácidos grasos presentes en mantequilla

Muestra ( mg/g) EI M11 mg/ mL DP mg /mL DE mg/mL

10 10 2
15 mg de mantquilla
mg / mL 3

Datos Area rel Con rel Factor rel

M14:1 0.10963356974 0.2 0.54816785
M16 1.19988507634 0.2 5.99942538
M16:1 1.25365293055 0.2 6.26826465
M18 1.15728123461 0.2 5.78640617
M18:1 1.26219011657 0.2 6.31095058
M18:2 1.1973403382 0.2 5.98670169
M18:3 0.96215728123 0.2 4.81078641
M11 12182 0.2 60910

De la muestra
Area rel Conc. Rel conc mg/mL mg mg /g
M14:1 0.12396829288 0.22615024 2.26150244 11.3075122 753.834148
M16 0.70605540574 0.11768717 1.17687172 5.88435859 392.290573
M16:1 0.70605540574 0.11263969 1.12639693 5.63198465 375.465643
M18 0.34624499469 0.05983766 0.59837658 2.99188291 199.458861
mayor M18:1 1.36373294108 0.21608994 2.16089941 10.8044971 720.299804 BIEN
M18:2 0.6796600474 0.1135283 1.13528297 5.67641485 378.427657
menor M18:3 0.15788183378 0.0328183 0.328183 1.64091502 109.394335 BIEN

M11 12237 0.20090297 2.00902972

Determinar el % en dos muestras

Datos de la disolucion estándar

DP mg/mL EI (mg/mL) DE mg/mL Area EtOH Area EI Area rel. conc rel
54.5 60 5.45 1105.81 1606.98 0.6881 0.09083333

Muestra mg/mL

Muestra 1 Peso mg Muestra 2 Peso mg Datos de la muestra

1a 135.72
1b 123.26 Disolucion Area EtOH Area EI
1c 150.28 1a 738.26 680.62
2a 116.75 1b 1085.65 1067.7
2b 156.35 1c 519.11 441.33
2c 204 2a 529.35 583.11
2b 1119.06 905.54
2c 488.67 320.83
Factor rel
7.57573526

Area rel. conc rel conc mg / mLm/m % m/m

1.0847 0.14317917 8.59075023 0.632976 63.2975997
1.0168 0.13421956 8.05317348 0.65334849 65.334849
1.1762 0.15526414 9.31584826 0.6198994 61.9899405
0.9078 0.11983057 7.18983442 0.61583164 61.5831642
1.2358 0.16312516 9.78750945 0.62599996 62.5999965
1.5231 0.20105548 12.0633289 0.59133965 59.133965
 Identificar e identificar
Alcanfor
Naftaleno

Datos

DE mg/mL areas factor resp

Alcanfor 2.3 56887110 24733526.1
Naftaleno 2.3 41962757 18244677

Muestra mg Alacanfor Naftalrno Con rel Al Conc Naf conc alcanfor mg/g
200 56389894 15492843 2.27989708 0.84917058 113.994854194562
naftaleno mg /g
42.4585292382
Disolvente en la muestra Xileno
Concentracion % Datos del estandar
Datos Disolvente
Acetona EI Xileno
Acetona
Muestra
mg /mL
20 Datos de la messtra
Disolvente
Xileno
acetona
atos del estandar
Area Areal conc % conc rel factor resp
12761 0.13079888 35 1.16666667 0.11211332
97562 30

atos de la messtra
area area rel conc rel conc% Conc final %
18792 1.52198915 13.575453 407.263591 20.3631795
12347
 Adiciones patrón

Acetaldehído
Adiciones patrón
120000
1g de queso
5 mL vol 100000
f(x) = 20311.5 x + 19958.4

80000
Volumenes de acetaldehido 10ppb
60000
Adicion ppb area
1 0 19998 40000
2 1 39899
3 2 61000 20000
4 3 81010
5 4 101000 0
0 0.5 1 1.5 2 2.5 3 3.5 4 4.5
ón

3 3.5 4 4.5
EI ng/mL
200
Areas
Nivel conc ng/mL OXI OC 2-EHMC AVO Nivel conc ng/mL
1 100 4706 38773 5369 5496 1 200
2 200 7085 64276 8829 8900 2 200
3 400 14973 140823 20768 19858 3 200
4 600 20968 214851 26804 28046 4 200
5 800 28103 313078 37583 40045 5 200
6 1000 36795 362708 47325 51351 6 200

Areas
Muestra OXI IAMC OC 2-EHMC AVO Conc rel Conc ng/ mL
A 10522 33791 88523 30521 13356
B 5073 34592 235621 10223 7806
C 24211 34004 324378 40945 43109

Muestra OXI IAMC OC 2-EHMC AVO

A 296.368969 33791 88523 30521 13356
B 142.888981 34592 235621 10223 7806
C 681.942019 34004 324378 40945 43109
EI Conc rel X Area rel OX Area rel OC Area rel EHMC Area rel AVO
34768 0.00287621 0.13535435 1.11519213 0.154423607915 0.1580763920847
33673 0.00593948 0.21040596 1.90882903 0.262198200339 0.2643067145784
34704 0.01152605 0.43144882 4.05783195 0.598432457354 0.5722106961734
34654 0.01731402 0.60506724 6.19989034 0.77347492353 0.809314941998
35801 0.02234574 0.78497807 8.74495126 1.049775145946 1.1185441747437
33882 0.0295142 1.08597485 10.7050351 1.396759341243 1.5155834956614

OX O
1.2
12

1 f(x) = 35.5025011153745 x + 0.0125330511912644 10 f(x) = 375.064271753529 x

R² = 0.996480559740522 R² = 0.994372915230484
0.8 8

0.6 6

0.4 4

0.2 2

0
0 0 0.005 0.01 0.015
0 0.005 0.01 0.015 0.02 0.025 0.03 0.035

AVO
EHMC
1.6
1.6
1.4 f(x) = 51.1576940328731 x − 0.0
1.4 R² = 0.99617502669445
f(x) = 46.5462944129611 x + 0.0114066454731298 1.2
1.2 R² = 0.995187716851937
1
1
0.8
0.8
0.6 0.6

0.4 0.4

0.2 0.2

0 0
0 0.005 0.01 0.015 0.02 0.025 0.03 0.035 0 0.005 0.01 0.015 0
 OC
12

10 f(x) = 375.064271753529 x − 0.140401398013988

R² = 0.994372915230484
8

0
0 0.005 0.01 0.015 0.02 0.025 0.03 0.035

AVO

f(x) = 51.1576940328731 x − 0.0235633368624186

R² = 0.99617502669445

0 0.005 0.01 0.015 0.02 0.025 0.03 0.035

Problema 23

Datos estandar
Area Vit E Area Vit A to tr vit A W 1/2 tr vit E
153587 143587 1.2 5.8 0.2 7.2

Vit E conc mg/ mL Vit A conc mg/ mL Fr vit E Fr vit A

10 10 15358.7 14358.7

Datos de la muestra
Area Vit E Vit E conc mg/ mL conc microg/mL g/g % peso
35186 1000 2.290949103765 0.00229095 0.22909491
W 1/2
0.3
determinar la conc de Muestra mg/ mL
Piroxicam mg/ mL Ibuprofeno mg/ mL 0.5
0.5 0.5 piroxi
Muestra 309819
Area piroxi Area ibuprofeno Conc 0.227832881814
Estandar 673446 346638 mg 0.455665763627
Estandar 673333 347894 113.9164409068
Estandar 692999 346663
Promedio 679926 347065 DUDA
Fr 1359852 694130

Chart Title
conc mg/ml area
1 0.000239 56066 400000

2 0.000478 116178 350000

f(x) = 242187208.607292 x − 578.599999999977
3 0.000717 173977 300000 R² = 0.999879610906364
4 0.000956 230044 250000
5 0.001195 290156 200000
6 0.001434 345645
150000
100000
0.0239
50000
muestra area 0
0 0.0002 0.0004 0.0006 0.0008 0.001 0.0012 0.0014
% v/v 172777
20

conc mg/ml

ACEtona EI %
5

Curva de calibracion
 C % ET C ACETINA %AREA ET AREA ACETONA
1 5 1320 7425
2.8 5 4851 8662
4.6 5 7590 8250
6.4 5 9504 7837
8.2 5 10824 6600
10 5 14850 7425

MUESTRA
area etoh area acetonaarea rel conc rel
1 10258 8121 1.26314493289 1.298557577272
2 10432 8121 1.284570865657 1.320584192445
3 5098 8301 0.614142874352 0.631360552738
4 6397 8248 0.775581959263 0.797325630474
5 9020 8251 1.093200824143 1.123849034824
6 11799 8325 1.417297297297 1.457031649125
7 10795 8324 1.296852474772 1.333210120129
 Ibuprofneo
177591
0.2558468875
0.5116937749
0.5116937749

Chart Title

7208.607292 x − 578.599999999977
9610906364

0006 0.0008 0.001 0.0012 0.0014 0.0016

AREA REL CONC REL fac resp
0.1777777778 0.2 0.88888889
0.5600323251 0.56 1.00005772
0.92 0.92 1
1.2127089447 1.28 0.94742886
1.64 1.64 1
2 2 1
0.97272925

conc %
6.4927878864 0.06492788 0.64927879 64.9278789
6.6029209622 0.06602921 0.6602921 66.0292096
3.1568027637 0.03156803 0.31568028 31.5680276
3.9866281524 0.03986628 0.39866282 39.8662815
5.6192451741 0.05619245 0.56192452 56.1924517
7.2851582456 0.07285158 0.72851582 72.8515825
6.6660506006 0.06666051 0.66660506 66.660506
prome 56.8708483
Curva

THC microg/mL area CURVA DE CALIBRAZION THC

1 11.8267
1000
5 71.1157
900 f(x) = 15.833714814192 x − 8.89135406087109
10 140.2586 R² = 0.999787361389686
800
30 470.289
700
60 940.427
600
500
400
300
200
100
0
0 10 20 30 40 50 60 70

Muestra Area conc Thc microg/mL

1 163.20067 10.802428739843
2 161.5121
3 161.75
promedio 162.15425666667
C

60 70
ng/mL
500

Antraceno Fluoranteno
Estándar 473424 246638
Estándar 473311 247894
Estándar 492977 246663
Muestra 1 328856 88394
Muestra 2 300098 89119
Muestra 3 328099 87932
Muestra mg/ mL Normalizacion de areas
0.5 Area de 1S 4R Area 1R 4S mg/mL 1S mg/mL 1R
46700.35 1868 96.15 3.85
Solución estándaar
mg/mL
2

Inyeccion muestra conc muestra mg Muestra areaInyeccion estandar Area Factor

1 200 232000 1 246638 123319
2 150 172846 2 247894 123947
3 300 347093 3 246663 123331.5
Conc muestra mg/ mL mg mg/ tableta
1.88129971861595 0.23516246 70.5487394
1.39451539771031 0.23241923 69.7257699
2.81430940189651 0.23452578 70.357735
PROM 70.2107481
menos a 0.05% m/m

Estandares Sulfato Sulfamato Muestra Area sulfato area sulfamato

mg/ mL 0.1 0.18 300.4567 112.459

Curva de calibración

Conc microg/mL Area ssulfamato area sulfato

1 11.8267 31.8267
5 59.1157 159.1157
10 110.2586 319.2586
20 236.289 636.289
30 354.427 954.427

Sulfamato sulfat
1200
400

350 1000
f(x) = 11.8588262436914 x − 2.15310641672679
R² = 0.999374748773457 f(x) = 31.8069156452776 x + 0.3321134823359
300 R² = 0.99999832320764
800
250
600
200

150 400

100 200
50
0
0 0 5 10 15
0 5 10 15 20 25 30 35
 conc sulfata microg/ conc sulfamato microg/mL microg microgramo g sulfato
9.43580343949445 9.66456699553082 94.35803439 96.64566996 9.43580344E-05

sulfato

452776 x + 0.332113482335956
320764

10 15 20 25 30 35
g sulfamato % sulfato % sulfamato
9.664566996E-05 0.047 0.048
muestra estandar muestra conc. factor r conc mg/ mL
mg/mL Fructosa 1200 1100 3 400 2.75
60 Sucrosa 900 870 2 450 1.93333333
lactulosa 600 155 1 600 0.25833333
Lactosa 710 720 1 710 1.01408451
% m/m
13.75
9.67
1.29
5.07