Universidad

Tecológica de Pereira
Chemical School
Laboratory of Instrumental Analysis
Activity: Polarimetry Applications
polarímetros
[H] EQUIPOS ÓPTICOS
Introduction
An instrumental analysis offers a variety of applications in food analysis,
olarímetro manual 404 environmental soil and water quality control, research and development among
others.
Polarimetry is the
polarímetro manual 404 de Zuzi es un instrumento robusto y
oldest and most widely used method constituting a
de desviar el plano de la luz polarizada. Esta característica es de gran
nonseparation
l de manejar, ideal para aplicaciones en la industria alimentaria,technique.
macéutica, química, etc., así como en educación. Determinadas
utilidad puesto queIt relies
la medición de laupon measurement
rotación óptica a través de un
polarímetro permite determinar la concentración, contenido y pureza de
of a net effect. Data from
tancias son óptimamente activas, espure enantiomers are
decir, presentan la capacidad required
la sustancia for comparison. The technique is based on the fact
ópticamente activa.

that chiral molecules rotate the plane of polarization of plane polarized light [1].

In quantitative analysis, it is understood that the
calibration curve must have at least 6
concentration levels where the concentrations
must cover a linear range between 50-150% in
relation to the expected results [2].



Figure 1. Polarímeter [3]


Prestaciones y accesorios incluidos en el equipo
Objective
To dedemonstrate
| Fuente de luz de vapor de sodio (longitud onda de 589.3 nm)
| Compartimento inclinado para muestras que permite alojar tubos de
the application
4| Ocular con ajuste dióptrico. of polarimetry: Physico-chemical evaluation of
5| Dos lupas laterales de 4 aumentos para facilitar la lectura de la escala.
asta 220 mm. Rosmarinus officinalis L. Essential oils [4].
| Doble escala de lectura mediante vernier para evitar diferencias por
6| Suministrado junto con dos tubos para muestras de 100 y 200 mm
respectivamente.
xcentricidad.
Instructions
Características técnicas
Read the paper carefully, answer the questions, and provide information about the
topics suggested:
Summarizes the analytical objective of the study,
Referencia
Nº escalas
HMB001
2
1. Summarizes the analytical objective of the study,
Rango de medición
División escala
±180º
1º
2.
Lectura mediante
Peso
Summarizes
vernier the
0.05ºconditions (Instrumentation and measurement conditions,
7.5 Kg
Sample
Dimensiones (LxAxH)
Alimentación
preparation
480x135x325 mm
CA220V, 50-60Hz
and analysis); this is concerning the Polarimetry
application.
3. Did the authors perform a quantitative analysis? If yes, provide information
about the quantitative method? (Calibration method, relationship between
optical rotation and concentration) Give the pieces of evidence in the paper.
4. ¿Why polarimetry can be useful in identifying composition and purity of
esssential oils?
5. Comment the type of information obtained in the discussion and in the
conclusions.
LARÍMETROS
EQUIPOS ÓPTICOS

a: el fabricante se reserva el derecho de modificar las características y fotografías de los productos
For the grading of this assignment, you need to send to the course: Instrumental
Analysis G2 google classrom:
The infographics, in pdf and in website (accesible version),

Delivery date: Monday March 08-2021. 6:00 pm.

References
1. Castro, E; Federman. Análisis-instrumental- Algunos-métodos- Fotométricos
y Electrométricos Apuntes de Clase.
2. Konieczka, P., & Namieśnik, J. (2018). Quality Assurance and Quality Control
in the Analytical Chemical Laboratory. Quality Assurance and Quality Control
in the Analytical Chemical Laboratory. CRC Press.
https://doi.org/10.1201/9781315295015
3. Polarímetro manual 404. Disponible en:
https://www.auxilab.es/controles/ObtenerPDF.ashx?f=%5c00%5c00%5cki
%5cfq_F_HMB001_C.pdf,
4. Atti-Santos, A. C., Rossato, M., Pauletti, G. F., Rota, L. D., Rech, J. C., Pansera, M.
R., … Moyna, P. (2005). Physico-chemical evaluation of Rosmarinus officinalis
L. Essential oils. Brazilian Archives of Biology and Technology, 48(6), 1035–
1039. https://doi.org/10.1590/S1516-89132005000800020,