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1 El sustrato de la amilasa es
Tu respuesta: almidón.
6 Las hidrolasas son enzimas que descomponen moléculas grandes en subunidades más
pequeñas mediante la adición de .
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2/9/2020 PhysioEx Exercise 8 Activity 1
1 Tubes 3 (amylase, starch, pH 7.0 buffer), 7 (amylase, starch, pH 2.0 buffer), and
8 (amylase, starch, pH 9.0 buffer) reveal that
2 This activity includes a number of negative controls. Which tube indicates that the
amylase solution was not contaminated with maltose?
3 Which tubes indicate that the deionized water did not contain contaminating starch or
maltose?
Correct answer: tubes 4 (amylase, deionized water, pH 7.0 buffer), 5 (deionized water,
starch, pH 7.0 buffer), and 6 (deionized water, maltose, pH 7.0 buffer).
Correct answer: Salivary amylase would be most active in the mouth because pH 7 is
where its peak activity is.
2 What effect did boiling have on enzyme activity? Why? How well did the results this
compare with your prediction?
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2/9/2020 PhysioEx Exercise 8 Activity 1
3 At what pH was the amylase activity the most active? Describe the significance of this
result.
4 Briefly describe the need for controls and give an example used in this activity.
5 Describe the significance of using a 37°C incubation temperature to test salivary amylase
activity.
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