Documentos de Académico
Documentos de Profesional
Documentos de Cultura
Conchita
Ramrez Gomez, hermana del ilustre profesor autor de este libro.
Como podremos comprobar a travs de su curriculum y de las reseas a las que se
hace referncia, as como en el contenido del presente documento ha sido uno de los
grandes cientficos y miclogos del siglo XX, habiendo sido declarado el Miclogo
nmero 1 en 1985 en el congreso internacional de Cientficos celebrado en Blgica.
Los honores todos para un hombre que naci en el pas y en la poca equivocada.
El resto de los pases con inters en su rama de investigacin se disputaron su
sabidura e intervencin hasta los ltimos das de su vida.
Dedicado a Da. Conchita Ramrez, mi mejor amiga. Cuya esperanza es que el
esfuerzo y saber de su hermano estuviera al servicio de toda la humanidad.
Motivacin que gui el trabajo y todo el esfuerzo del sabio a lo largo de los aos
de dedicacin absoluta a la investigacin.
CURRICULUM VITAE
Actualizado al ao 1984
CARLOS RAMIREZ GOMEZ
Realizado por l mismo en vida.
Universidad Comptutense
DOCTOR EN CIENCIAS NATURALES, Universidad Complutense
microorganismos.
1971 - Nombrado Profesor de Investigacin del C.S.I.C.
1972 - se traslada a Lille, donde participa a un curso de un mes en el Instituto Pasteur dirigido por
el Prof. Buttiaux, sobre Microbiologa de los Alimentos.
Seguidamente y por tres meses, se traslada al Institituto de Microbiologa Tcnica y de los Alimentos
(TNO) de Holanda en Zeist, para realizar estudios sobre micotoxinas y su deteccin en los alimentos y
piensos.
(Para aos siguientes vanse las pginas de publlcaciones, etc.
SOBRE
TECNICAS MICROFUNGICAS.
SOBRE FERMENTACIONES.
Uno en Microbiologa Espaola.
SOBRE MICOTOXINAS
Cinco trabajos: dos en revistas espaolas y tres en extranjeras.
TRES MONOGRAFIAS
LOS PSILIDOS DE ESPAA" Bol. Real. Soc. Esp. Hist. Nat. Vo. 55, 56, y 57, 210
pg. 1955, 1956, 1959.
"MANUAL AND ATLAS OF THE PENICILLIA" 1982. Elsevier PuB. Co Amsterdam. XV + 874
pgs.T
TRABAJOS EN COLABORACIN
X) Sa colaboraciSn con LA SECCIN DE MICRQBIOLOGA TECNICA, se estaa reali-zaado estuciios sobre
el pool de amiaoScidos eh los HALOBACTSRIUM, con el fia de tener mas datos para peraiitir situar
estos microorgaaismos ek el lugar que les corresponda taxonomicamente*
2)
Coa la SEC-ION DE FIJACION DEL NITROGENO se esta estudiando el papel que
desempenaa LQ5 HQKGQS SUPERIORES en la utilizacion total de las ligainas, prod
ducto dc desehcho de las papeleras*
3)
Ea colaboracion coa un medico Alerg6logo de las Palmas de Gran Canaria, se
esta 11 vando ua ESTUDIO ECOLOGICO DEL MEDIO AMBIENTE de esta Capital, en
cuaato a la MICOFLO.U CONTAMINANTS DEL AI3E Y SU INFLUENCIA p LAS ALS3fi*AS |
7/ Referencias de publicaclones o Tesis doctorales en relacin con el Proyecto:
Sobre tazonoma de mohos y ecologa mlcolgca ha pubilcado veinte trabajos. Sobre fisiologa
da mohos ha pubilcado diez trabajos. Sobre accin de las radiaccines gamma sbre la esporulacin
pubilcado un trabajo. Sobre metabolismo de Mohos ha pubilcado cuatro trabajos.Sobre
Micotoxinas ha presentado dos trabajos en el Congreso Nacional de Mlcrobiologa da Granada
(1973), dos en el de Salamanca (1975), uno en el International Symposium on Mycotoxins in
Foodstuffs da Paris (1976). De los trabajos pubilcados, 44 tratan de temas de Micologa General
y Aplicada. Dos Tesis doctorales presentadas y una on preparacin
tratan de la micoflora micotoxignica contaminante de produccin destinados a la alimentacin
humana.
Repercusin
Trataremos do reunir en este apartado, aunque no exaustivamente la repercusn Internacfonal
de nuestros trabajos, que se hace bien patento
en las numerosas menciones en revistas extranjeas y en tratados de nuestra especialidad:
1954,* J.Bofdn et F,Abadie.Bull.Soc.Mycol.France,70: 353-383;1957, J.Boidin et J.M. Adzet,
Bui|iSec, MymlFrwice, 73:331*342; 1958, "The Chemistry and Biology of Yeasts, A.H. Cook
(editor); 1959 Van der Walt,
J.P. Antony Van Leewenhoek 25: 458-464; 1962, Artagaveitia-Allende, R.C.
y C. Leganani.
Micopathologa et Mycologa Appl. 18: 140-146; 1962, L. de mONTEMAYOR
y B. Heredia de Ganero, Mycopatholga et Mycologa Appl. 18: 1-52; 1962,
Artagaveita-Allende, B.C. Mycopathologa et Mycologa Appl. 25: 299-302; 1962, M.
W.Miller, H. J. Phaff and H.e.Synder. Mycopatologa Appl. 16: 1-18; 1965 J. Boldin
J. Abadie et Y. Lehoday. Bull. Soc. Mycol. France 81: 5-23 1965, M.C.Pignal et J. Boldfn, Dull.
Soc.Mycol,France SI; 197-226; 1965, D, Yarrow..Ant, van ' oe Leewenhoek 3It 405-467;
f965,R*C#Artagaceftfe>AMende,Myoop*thologa et Mycologfa Appl* 2S;237*273; 196> J.Bofdfn,J,
Plgnal et M.Besson, Bull, Soc.Mycol.France 8tj566*60@; 1966 "The Fungi", An advanced Treatise,
Ed,G, C *Afnsworth and A#S#Sueeman,Vot* I pag 135-I4tj 1966, G,ffiurtan en "The Fungi", Vol,
II pgs.367; 1967* J. J, Miller, Bull,Sec,Hist, Natur.Toulouse,Francef 103;3a7*339; 1964,
J.Bo!dln,F.Abadle et Y, Leho* dey et M.C Filial,Buff.Soc,MycoUFrence, 80*397*438j W7,R.Moniro*
cher, Bvtil Soc.Mycol.Framce 83*641-730 J 1968, F. Abedle,My wathologle et Mycologfa Appl.
36i81*t3j 1968, MlNor^M* liter notional Atomic Ertorgy Agency,Vlnna pg. 181-10*1 J* J* Milter
and G^offman-Ostenhoff, ZeftachrM fur AHgemelne Mfkroblofogfe, 44t63*294f If 69, J,J. Ml Her
on "The Yeasts", EoiA^H.Rdfi and J, S.HarHaon, Aeectemlc Press* Vol I Pfl. 93-104*334*381} 1970,
"The Yeasta* A Teconomic Study, Lodde*, J.Ed.pgs ||fiIM,)7fi.861l1Pti.lofri 13SU
)NGOS PUBLICADAa
1984
CARLOS RAMIREZ
PUQLICACIONES
1949.- Ramirez C. Nota sobre hall
.azgo de "Acarapis woodit" (Acarioais de las abejas) en la isla de Malloroa. Bol. Real. Soc.
Hist. Nat, Madrid. - Ramirez, C, Socias, A, e
|^ 1955
/^1956. W 1956.
/ 1956
I 1952
j 1952 4 1953
1953.
C 1953. 1 1953.-? 1954.-^ 1954.< W.9 54.I 1954.)Vl955.47 611-118. Gil. Presencia de Aspergillus y Penicillium en esputos y orina de tuberculoses.
Micro- biologa Esp. 5: 113-121.
1
Ramirez, C. Une nouvelle espece de Sporotriohum. le Sporotrichum sanguineum. Revue de Mycologie. Paris. 17j 214-218
Trois nouvelles especes de levures isolees de liqueur tan-nantes. Revue de Mycol. Paris. 18'!
149-158.
Ramirez C. Introduccin de una modificacin al metodo de Wickerham para determinar el poder
fermentativo de la rafi-nosa por las levaduras. Estratto degli atti del VI Congresso
Internazionale di Microbiologa. Roma. Septiembre. I: 872-873 Ramirez, C. Contribucion a la
tincin de ascosporas y clami-dosporas de las levaduras. Microbiol. Esp. 6: 239-246.
Estudio de nuevas especies de levaduras ai&ladas de distintos sustratos. Microbiol. Esp. 6:
249-253.
Deux nouvelles especes de levures isolees de divers substrats. A Revue de Mycologie (Paris).
19: 98-102.
Ramirez, C., et J. Boidin. Saccharomyces chambardii, nouvelle J
espece de levure isolee de liqueur tannante. Bull. Mens. Soc.
Linn. Lyon. 23: 151-152.
Ramirez, C. Socias, A, y Pelaez Campomanes, F. Una nueva eg- i pecie de Debaryomyces. El
Debaryomyces toletanus. Microbiol. Esp. 7: 111-114.
Ramirez, C, Genestar, R., y Socias A. Una nueva especie de "7 Endomycopsis: Endomycopsis balearica
aislada de concentrado de tanino de encina. Microbiol. Esp. 7: 107-110. Ramirez, C. "Los Psilidos
de Espafia". I. Bol. Real. Soc. Esp. Hist. Nat. 53: 151-218.
Ramirez, C. y Socias A. Estudio cariologico de algunas levaduras ascosporogenas y
anscosporogenas. Microbiol. Esp. 8: 389417.
Ramirez, C. "Los Psilidos de Espafia". II. Bol. Real Soc. Hist.
Nat. 54: 63-106.
Ramirez C, Pelaez Campomanes, F. El Sporobolomyces hispani^jis
so. nov., nueva especie de Sporobolomyces aislado de extracto
de corteza de encina. Microbiol. Esp. 9: 37:41.
Ramirez, C. y Sierra, G. Una nueva variedad de Torulopsis holmii (Jorgensen) y estudio de sus productos metabolicos.
Microbiol. Esp. 9: 147-162.
II 1956 /? 1957
tf 1957. 7" 1957.
$1 1959.
^ 1959.
-?3 1959.
Zh 1960.2;,-1960. 1963. -Jl 1964.^^T 1964.-4^1965.-%t> 1968.-}( 1968.-3 ^1969.13 1970.-3^1972..- Ramirez, C, y Pelaez Campomanes, F. Una nueva especie dP
Endomyces: Endomyces ovetensis. MierobioX. Esp. 9: 3.91-200 ' *
. - Ramirez, C. Contribucin al estudio de la ecologa de las
levaduras. I. Levaduras aisladas de hongos carnosos. Microbiol ,.
Esp. 10: 215-247. "-"-croDiol. )|
elemen-Microbiol.
Real Soc.
Ramirez, C. Nota sobre algunas modifieacione* introducldas a la tincin de nucleos de
hongos. Microbiol. Esp, 10: 201-203
Ramirez, C. y Socias, A. Cromosomas ligados y Mitosis tales en los nucleos de Aspergillus
y Penicillimjj Esp. 10: 259-286.
Ramirez, C. "LOS PSILID0S DE ESPANA". III. Bol Esp. Hist. Nat. 57: 5-87.
Ramirez, C, et Chodat, F. Etudes sur la periodicite des echan-ges gazeux chez une levure:
Kloeckera apiculata. Microbiol. Esp. 12: 265-296.
Ramirez, C. Methode rapide et simple pour determiner le degre
de fermentation du raffinose par les levures. Ant. van Leeuwenhoek. 25: 445-448.
Ramirez, C. Une nouvelle diagnose d'une levure isolee d'Amanita JTJ livido-palescens:
Geotrichum zambettakesii (Ramirez) comb nov. Revue de Mycol. (Paris). 25: 433-436.
Ramirez, C, and Miller, J. J. Observations on vegetative nuclear division inScJccharomyces
cerevisiae. Can. J. Microbiol. 8: 603-608.
Ramirez, C, and Miller, J. J. Accumulation of free proline during yeast sporo^enesis.
Nature. 197: 722-723. Ramirez, C. Studies on the causes of reciprocal inhibition of
pseudomycelium formation between parallel colonies of yeasts of the genus Candida. Can.
J. Microbiol. 10: 931-933. Ramirez, C, and Miller, J. J. The metabolism of yeast
spo-rulation. VI. The changes in amino acid content during sporo-genesis. Can. J.
Microbiol. 10: 223-231.
Miller, J. J., Kingsley, V., and Ramirez, C. Comparative sensitivity to Gamma radiation
of yeast mitosis and meiosis. Radiation Bot. 5: 384-390.
Ramirez, C, and Ransom, J. P. The growth of Candida albicans and Salmonella typhimurium
in mixed cultures. Microbiol. Esp.
21: 77-88.
Ramirez, C, Ransom, J. P., and Pitkin, D. R. The in Vitro Interaction of Candida albicans
and Salmonella typhimurium. Microbiol. Esp. 21: 88-91.
Ramirez C. Antibacterial action of non-volatile substances extracted from Artemisia
tridentata Nutt. ssp. tridentata. Can. J. Microbiol. 15: 1341.
'Uamivez, C, Rodriguez, D., and Lanzuela, F. Rapid diagnosis of Candida albicans. Mycopath.
et Mycol. Appl. 40: 10?-111. Ramirez C, and Gonzalez, C. Candida iberlca sp nov. A new
U species of yeast Isolated from Spanish sausages* Can. J. Micro** biol. 18: 1778-1780.
4
b 1979.Martinez, A.T., and Ramirez, | Ecological importance of antibiosis in soil. Production of antibiotic
by species of Penicillium. Anales Edaf. Agrobiol 3s-159-166.
!T t 1980.Torres, J., Guarro, Suarez, G, Sufie , N., C^lvo, M.A.
and Ramirez, C. Morphological changes in strains of'LpergilluB flavus Link ex Fries, and A. parasiticus SpeaTeT
related with aflatoxin production. Mycopathologa: 72*'
171-174.
Tl 1980
i'^> 1980.
| r 1980.
2T & 1980.
Calvo, M.A., Guarro, J., Su&rez, G., and Ramirez, C. Airborne Fungi in Barcelona City (Spain). I.
A two-year study (1976-1978). Mycopathologa. 71: 89-93. S'3 1980.- Calvo, M.A., Guarro, J. Suarez,
G., and Ramirez, C. Airborne Fungi in the air of Barcelona, Spain. V. Studies of the spore content
of air in dwellings. Ann. Allergy. 44; 228-234.
Ramirez, C, and Martinez. A.T., and Berenguer, J. Four ^ ^ new species of Penicillium isolated from
the air. Mycopathologa 72: 27-34. Ramirez, C., and Martinez, A.T. Some new species of Penici- ^
Ilium recovered from the atmosphere in Madrid and from
other substrata. Mycopathologa. 72: 181-191.
Guarro, J., Calvo, M.A., and Ramirez, C. Soil Ascomycetes
(Sphaeriales) from Catalonia, Spain. I. The genus Chaeto-mium Kunze ex Fresenius. Nova Hedwigia.
28: 191-216.
iT 7 1980.- Calvo, M.A. , Guarro, J., Suarez, G., and Ramirez, C. Airborne Fungi in the air of Barcelona
(Spain). 111= The ge-nusAspergillus Link ex Fries. Mycopathologa. 71: 41-43.
$ <i 1981.
$T / 1980.- Calvo, M.A. , Guarro, J., Suarez, G., and Ramirez, C. Airborne Fungi in the air of Barcelona
(Spain). IV. Various isolated genera. Mycopathologa. 71: 119-123. Ramirez, C, and Martinez, A.T.,
Seven new species of Penicillium and one variety of Penicillium novae-caledoniae Smith. Mycopathologa. . 74: 35-49.
o
1981./
1981.(^
1982.^ 2
1982.Ramirez, C, and Martinez, A.T. Four new species of Penicillium isolated from different substrata.
Mycopathologa 74: 163-171.
Ramirez, C, Gonzalez, C, and Gutierrez, C. Rhodotorula matritense sp. nov., isolated from red pepper.
Mycopathologa. 74: 173-175.
Martinez, A.T., Calvo, M.A., and Ramirez, C. Scanning electron microscopy of Penicillium conidia.
Ant. v. Lee-wenhoek. 48: 245-255, 1982.
Ramirez, C. "MANUAL AND ATLAS OF THE PENICILLIA". Elsevier Biomedical Press B.V. Amsterdam. XV +
874 pp.
&H
1983.
if
1983.
U
1984.
61
1984.
xpensive method for>
Ramirez, C. A convenient and ine preparing fungal conidia for scanning
electron Mycopathologa 83: 17-18. m
Martinez, A.T. and Ramirez, C. Rhizosphaera oudemansii (Sphaeropsidales)
associated with needle cast of Spanish
Abies- pinsapo. Mycopathologa. 83: 175-182.
Ramirez, C, and Gonzalez, C. Penicillium flavido-stipj-tatum sp. nov., isolated from sand dunes at
the Arches National Monument, Utah, U.S.A. Mycopathologa 88:3-7
Ramirez, C, and Gonzalez, A. Rhodotorula grinbergsii I sp. nov. isolated from decayed wood in the
evergreen rainy Valdivian forest of southern Chile. Mycopathologa 88: 51-53.
i^ 1984. 1 1984.
1984.- Ramirez, C, and Gonzalez, A.. Two new species and one variety of nitrate-utilizing,
mycelial Candida isolated from decayed wood in the evergreen rainy Valdivian forest of
bfrSoutsMign Chilpa I M$j{fcop&thologa& 88 : 55-60.
3^
Ramirez, C., and Muntanola-Cvetovic, M. Penicillium ju-goslavicum sp. nov., isolated from sun
flower seeds (Helianthus annuus L.). Mycopathologa 88: 65-69 Ramirez, C., and Gonzalez, A.
Pichia scaptomyzae sp. nov. isolated from Scaptomyza multispinosa Malloch (Diptera) in southern
Chile. Mycopathologa. 88: 79-81.
If 1984.- Ramirez, C, and Gonzalez, A. Five new filamentous, glucose-fermenting Candida
isolated from decayed wood in the evergreen rainy Valdivian forest of southern Chile. Mycopathologa. 88: 83-92.
73 1984
71 1984.- Ramirez, C, and Gonzalez, A. Two new, amycelial Candida isolated from decayed wood
in the evergreen Valdivian forest of southern Chile. Mycopathologa. 88: 99-103. Ramirez, C. Validation
of Candida citrica sp. nov. (Furu-kuwa, Matsuyoshi, Minoda & Yamada). Mycopathologa. 86: 125.
7 4 1984.
Ramirez, C., and Gonzalez, A. Candida santjacobensist and
Candida ancudensis, two new species of yeasts isolated
from decayed wood in the evergreen rainy Valdivian forest
of southern Chile. Mycopathologa 88: 105-109.
Jf 1984.Ramirez, C, and Gonzalez, A. Two new filamentous, non
fermenting Candida and a new Schizoblastosporion isolated
isolated from decayed wood in the evergreen Valdivian
forest of southern Chile. Mycopathologa 88: 165-171.
li 1984.
Ramirez, C, and Gonzalez, A. Two new filamentous, fermenting ^
Candida isolated from decayed wood in the evergreen Valdivian
forest of southern Chile. Mycopathologa. 88: 185-189.
CONGRESOS .wINTERNACIONALES
1953.- Ramirez, C. Introduction de una modificacin al metodo de
Wickerham para determinar el poder fermentativo de la rafino-sa por las levaduras. Estratto degli
Atti dei VI Congresso Internazionale di Microbiologa. ROMA. Sep. I: 872-873
1963.- Ramirez, C, Friis, J., and U. Leupold. Allelic recombination and complementation in
adenine-requiring mutants of Schizosaccharomyces pombe. Proceedings of the XI Internal Congress
of Genetics. THE HAGUE, The Netherlands. Sept. I: 7.
1973.- Gonzalez, C., Gutierrez, C., and Ramirez, C. Effet du pH, du NaCl et de la temperature sur
la synthese et sur l1action des esterases chez Halobacterium. Proceedings of the 1st International
Congress of Bacteriology. JERUSALEM. ISRAEL. Sept.
1974.- Ramirez, C., Gutierrez, C, and G onzalez C. , The G^enus Detail
ryomyces: Revision of the species includec in the genus under t the light of new criteria. IV
International Symposium on Yeasts. VIENNA, AUSTRIA. June. I: E-8
1974.- Ramirez, C., and Moriyon I. New sppcies osf yeast isolated from an acid washed brown soil.
IV International Symposium on Yeasts. VIENNA, AUSTRIA. July. I: E-5. 8-12 JULY.
1976.- Ramirez, C, and Guergue, J. M. Incidence of Aflatoxin potential contamination in Spanish
sausages. Ill International Symposium on Mycotoxins in Foodstuffs. PARIS, FRANCIA. 16-18 Sept.
I: 10.
1977.- Ramirez, C, and Martinez, A.T. A new species of yeast
isolated from beech forest soil. V. International Specialized Symposium on Yeasts. KEZTHELY,
HUNGRIA. Sept. 12-15.
1978.- Ramirez, C, Martinez, A. T., and Ferrer Soledad. Three
new species of Penicillium. XII INTERNATIONAL C0NGR&# OF MICROBIOLOGY. MUNICH, ALEMANIA FEDERAL.
3-8 Sep. J; C*Z1
1981.- (Rarxtibtteztf JCU;, and Martinet, A. T. Contribution to the
Preparation Techniques of conidia for scanning electron microscopy. IV INTERNATIONAL CONFERENCE
ON CULTURE
19-25. P
C. adn Ramirez, C. The THE HAGUE. 15-17 Sept.
COLLECTIONS. BRNO, CHECOSLOVAQUIA. July
1981.- Gonzalez, C., Gutierrez of indicators organism*
TIONAL SYMPOSIUM OF THE FEMS
si
Snificane
I INTERNA1983.- Ramirez, C. amd Gonzalez, A. New species of yeasts isolated from Chilean "palo podrido"
in the rainy VaXdivian Forest IX INTERNATIONAL SPECIALIZED SYMPOSIUM ON YEASTS. SMOLEMICE
CHECOSLOVAQUIA. Abril 18-22. Abstracts: 25-26, -<'.
1983.- Ramirez, C. Ill INTERNATIONAL MYCOLOGICAL CONGRESS, 28-Agosto 3 Sept. TOKYO, JAPON.
Invitado a presentar una ponencia sobre "SPECIES CONCEPT IN PENICILLIUM BASED ON
MORPHOLOGICAL CHARACTERS", en el Symposium on Taxonomy of Food-borne Fungi. Abstracts: 247.
1984.- Ramirez, C. Two new species of Penicillium found in Tierra del Fuego. V INTERNATIONAL
CONGRESS OF CULTURE COLLECTIONS. BANGKOK, THAILAND. 2 5-Noviembre al 1 de Diciembre.
1983,- Martinez, A.T. y C. Ramirez. Effect of wil<fcfire on soil mi-crofungi in a Spanish
coniferous forest. Ill INTERNATIONAL MYCOLOGICAL CONGRESS. 28 Ag.-3 Sept. TOKYO, Japon. Panel
y Abstracts: 541
+ + + + +
1982.- Ramirez, C. Invitado por la USSR ACADEMY OF SCIENCES a la presentacin de su
libro"MANUAL AND ATLAS OF THE PENICILLIA". MOSCU, URRS. Noviembre 1982.
1983.- Ramirez, C. Invitado por el Comite Organizador del III INTERNATIONAL CONGRESS OF
MYCOLOGY, 28 Agosto- 3 Sept. como ponente en el Symposium on Taxonomy of Food-borne Fungi,
presentando la ponencia "SPECIES CONCEPT IN PENICILLIUM BASED ON MORPHOLOQMAL CHARACTERS".
TOKYO 1^ 1985.- Invitado a partidipar en el FIRST INTERNATIONAL PENICILLIUM
AND ASPERGILLUS N.A.T.O. A.R. WORKSHOP. Amsterdam 6-10 mayo.
presentando varias ponencias publicadas en "Advances in PeniciIlium Systematica" (Samson*R.A. and Pitt, J.I. Eds.)483 pp
London and New York. Plenum Publishing Co.
CONGRESOS NACIONALES
9
1971.- Solana, A, , Mo ran. C Mnfin? r D' , XT.. , "Ulan, u, iviunoz, c, Ramirez, C, y Gonzalez, C. "Aislamiento e identificacion
de levadu-ras de muestras de salchichones curados", in CON GRESO NACIONAL DE
MICROBIOLOGA. Barcelona, 2-3 Junio. Resumenes. I; 15.
1971.- Munoz, C., Moran, C, Solana, A,, Gonzalez, C, y Ramirez, C. "Poblacin bacteriana
en distintas muestras de salchichones curados". Ill CONGRESO NACIONAL DE MICROBIOLOGA.
Barcelona, 2-3 Junio. Resumenes. I: 129.
1973.- R amirez, C., Echarri, L., y Sanz, J. "Aislamiento y clasificacion de la micoflora
de especias comer-ciales". IV CONGRESO NACIONAL DE MICROBIOLOGA. Granada. Septiembre.
Resumenes. I: 7
1973.- Sesma, B., Ramirez, C., y Rodriguez-Burgos, A. "Estudio de las diferentes
especies microbianas que condicionan la maduracion de algunos embuti-dos." IV CONGRESO
NACIONAL DE MICROBIOLOGA. Granada. 8 de Septiembre. Resumenes. I: 20.
1975.- Gutierrez, C, Gonzalez, C, y Ramirez, C. "Nuevas aportaciones a la fisiologa
y bioquimica de la fa-milia Halobacteriaceae. V CONGRESO NACIONAL DE MICROBIOLOGA.
1-4 Octubre. Salamanca. Resumenes. I: 54.
1975.- Martinez, A. T. y Ramirez, C, "Estudio de la Comu-nidad microfungica de un suelo
Andico. Conslflflera-ciones generales. Estudio taxonomico y ecologico del genero
Penicillium. V CONGRESO NACIONAL DE MICROBIOLOGA, Salamanca, 1-4 Octubre. ResumenesI:
C-69
1976.- Gutierrez, C, Gonzalez, C. y Ramirez, C. "Asimila-cion de hidratos de carbono
por bacteri&s halofilas estrictas valoradas por auxanogramas". Ill REUNION CIENTIFICA
DE LA SECCIN REGIONAL DEL NOROESTE DE LA SOCIEDAD ESPANOLA DE MICROBIOLOGA. Oviedo.
4-5 Junio. Resiimenes. I: 124.
1977 _ Martinez, A. T. y Ramirez, C. Importancia ecologica en los medios naturales del
fenomeno de la antibiosis: produccin de antibi6ticos por cepas de Peni~
AU t^ I ^A
UHL
lAAA
un Andofuelo
1 argo *A,
Santia1977. -
iium. VI CONGRESO NACIONAT nt, . _____ go de Compostela. 6-9 j^8 MIC*BI0L0GIA. SanJ
Martinez, A.T. y Ramirez, C mMM
dad de los hongos microsc6picos en ** l* Com"**~
Variaciones a lo largo del perfil "" 7M~w,*,aei0' Wm aficrf, VI CONGRESO NACIONAL DE MICROBIOLO^*
de Compostela: 6-9 Julio.
1977. -
nov
gfierrez, C, Gonzalez, C. y Ramirez, I vi CONGRESO NACIONAL DE MICROBIOLoga. Santiago de Compostela
"Halobacterium vallismorti SD
bacteria halofila extrema, asimiladora de hidratos de carbono". Resumenes. I: 111.
1979
Martinez, A.JT., y Ramirez, C. "Tipo de ornamenta cion superficial de los conidios de Penicill
VII CONGRESO NACIONAL DE MICROBIOLoga. Cadiz, 24 Septierobre al 1 de Octubre .
1984.-
Gonzalez, A., Ramirez, C. y Grinbergs, J. "Palo po drido". Una transformacion biologica de la madera
animal, i II REUNION CONJUNTA DE MICO-LOGA. 31 Mayo-2 Junio, Bellaterra (Barcelona).
au &*&%*- ^vt-^
' I/A**? lf-*> gC-V **>
SL*-4A^L^M &^> ht *-k & %
&\^
CONSIDERACIONES ESTADISTICAS
sobre mi dedicacion exclusiva y total a i Tnwo tigacion durante el periodo de 15 aos (1968-iqa/n
Horas de trabajo obligatorias al afio: Horas de trabajo obligatorio
en 15 aos:
Horas que trabajo por ano:
Horas que he trabajado en 15 aos:
Horas extras por ano: Horas extras en 15 aos:
^Cuantos aos se necesitarian para traba-jar 51.960 horas de modo
normal?
Labor real en aos que he realizado hasta
ahora:
i
u
3.464
51.960
t !
27 aos y 6 meses
42 aos.
80.160
2.8
Ritmo de trabajo que supone
28.200 Lo que supone un ritmo de casi tres veces el normal
2 7 aos y 6 meses.
Afios extras que he regalado al Consejo en 15 aos :
42 aos.
Afios oficiales de trabajo que fcubiesen sido necesarios para realizar la labor
efectuada en estos 15 aos:
2.010
;En que ano nos encontrariamos actualmente si toda esa labor hubiese sido hecha
de modo normal?
89 aos.
,Que edad tendria?
59 millones 400.000 pt
^Cuanto me deberia el Consejo por todas las horas extra en 15 aos?
Contents
Preface
xi
xiii
Introduction
description of species . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Chapter 2
15
Examination of cultures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
17
21
22
Preservation of penicillia . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
23
Part 11
Chapter 3
The Monoverticillata . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Chapter 4
The Asymmetrica
201
Chapter
Asymmetrica-velutina sub-section
345
Chapter 6
Asymmetrica-Ianata sub-section
Chapter
Asymmetrica-funiculosa sub-section
437
447
....................
27
419
Chapter 8
Chapter 9
507
Chapter 10
The Polyverticillata
. . ..
609
Chapter 11
619
,.
,
.
631
641
647
649
653
665
Colour piates
673
Alphabetic list of the colour plates of the Penlcllliurn species and varieties .
801
805
845
849
o.
861
865
Preface
'1'111': PURPOSE OF THIS 'MANUAL AND ATLAS OF THE PENICILLIA ' IS TO SUMMARIZE
Xll
Abbreviations of International
Culture Collections
A T e c :
III> UN :
111 (; :
III1IM :
IIKM :
IIIU . :
C IIS :
CC M :
<:I-: H:
C I. M R:
CM I :
CMT :
CS IR :
DAOM :
EST .
AG R .
NAe. :
ETI-I:
FI E:
FGSe :
FP RL:
II Aee :
IAM :
IARI :
rcr:
11'0 :
lB S :
IJ FM :
IMI :
I MR U :
I M UR :
IC C:
IOC:
I P:
IPO :
ISP :
KNC
&
SF :
LC:
LCF:
L M:
LP :
LS H:
U K.
MN HN:
M W:
NC I B :
NCTC:
NCYC:
NHL:
NR R L:
NYSDH:
OEC D :
PC :
PO:
P RL :
QM:
IO,A :
'I'llTC:
I IAMII :
un:
l1SSR ,
A lU :
WIl :
e
o
en
en
U
::J
en
.
"'C
ro
"
Q)
eQ)
CD
ro
1:
a..
.l
haptcr 1
Introduction
' I'hcy coustitu to ih c norm al ami a n nh undu nt purt 01' (he mycollu rn 01' nll soils,
th cy play a cti ve mi es in lllallY d ccomposition a nd d ct crioruti ou pmcesscs , th cy
attack man and his dornesti c a n irna ls, and th ey are arnong th e most corn mo n
01' all labora to ry co nta m inan ts . Bcginnin g in th e lat e lBOOs so rne 01' thcm wer c
found to produce interestin g m etabolic produ ct s a nd tod a y a co ns ide ra ble
number 01' spec ies a re used in indu stry 1'01' th e produ ction 01' food specialties,
organ ic ac ids, e nzymes, and a n tibiotics . T h us, m an y peopl e train ed in
differ en t di sciplines a nd workin g in ve ry di ver se fields , mu st conce rn
th emselves w ith spec ies 01' Penicillium. For th ese peopl e , few 01' wh om a re
spec ia lists in m ycological ta xonomy, th ere mu st be av ail able a means 01'
identifying th eir specime n th at is under stable a n d easy to use , an d whi ch at th e
sa me time d oes not viola te th e basi c principI es a nd objective s 01' sound
bot anical nomenclature .
;)
t ypificd th c gcnus Pen icillium Link, liut su ch typifi cation is not in accordan cc
with the l nt crn utional C ode 01' Bolanical Nom cu clutu rc (St nllcu 1972) .
Accordin g lo Arti clc 1:1 1', thc d esi gnat ion 01' P. ctustaceum Linn acus ex Fri es as
I ype mu st be foll ow ed, becau se Fries (18 32) in th e Systema Myeologieum vol. 3 ,
aeeepted Link's ge n us Penicillium, hut design at ed P. erustaeeum Linnaeus ex
Fri es , based on Mu eor crustaccus L. (with the syno ny ms P. glaueum Link and P.
expansum Link) as the typ e spec ies of th e genus . Linnaeu s based hi s Mu eor
crustaceus on M ieh eli ' s (1 729) ' Botrytis non ramosa, alba, seminibus rotundis ' and
referred to plate 9 1, lig . 3 01' Mieheli's iUustrations . This figure possibly
illu strat es a Botrytis-like er us tose fu ngus whi eh shows sim ila rities to P.
exponsum . Hawksworth a nd Pitt ( 1976) th erefor e proposed th e eonserva tion of
Penieillium Link as th e leetotype . They d esignat ed a neotyp e IMI 39 76 1 = CRS
:125 .48 = Thorn 4852 of P. expa nsum, whi eh becomes the neot yp e of th e
conse rve d ge n us .
No perfeet state is known in P. expansum nor in relat ed spee ies. The gene rie
llame Penieillium is based on an imperfect fun gus and eonseque n tly it
represen ts a ge nus of th e H yphorn ycet es.
H owever, R ap er (19 57) con tes ted th e desirability o f applyin g dual
nom en d ature to PeniciUia , em p has izing the unity 01' all PeniciUia a nd insistin g
th at the ge n us Penicillium sho uld indude both ascogeno us and anascogenous
spec ies. Eve n tho ug h induding both perfeet a nd imperfect spec ies has its
advantages, in accordance with the Intern ation al Code 01' Bot anical
Nomendature (Stafleu 197 2) (A r t. 59) th e ge n us Penicillium sho uld be
res tric ted to th e a nascospo ric species.
The ' M a n ual o f th e Penieillia ' (Rap er a nd Thom 1949), whi ch d escribes 99
accepted sp ecies and varieti es of Penieillium sen su stricto and list s alm ost 700
spec ies, stiU remains now adays th e sta nd ard a nd indisp ens abl e guide to th e
taxon orn y of th e ge n us, d espite a pleth ora o f new species described since its
publica tion . R aper a nd Thorns grasp of relation ships with in th e ge nus was
ex traord inary a nd, by reducing more th an 700 species lo synon ym y , and
forrnin g th e remainder into se ries, th ey m ade it possibl e for erud ite workers to
be reason ably co nfide n t of m ost identification s. H ow ever, beeause 01' th e
paucity of obv ious taxonomic characte rs, and th e arbitrary nature 01' many 01'
th e M anual's gro upings, th e genus Penieillium stiU rem ains taxonom ica lly
difficul t , eve n 1'01' ex pe rts .
M an y new , add itional, species a nd varieties have been deseribed in th e
liter ature, representing sorn e 140 Peni ciUia, and various new surveys have
been published (Abe 1956 ; Kulik 1968 ; Pidoplicko 1972 ; Sam son et al. 1976 ;
Fassatiov 1977 ; Pitt 1979b) in th e yea rs sin ce the ' Man ual' was published ,
and so it is ob viou sly now ou t 01' d at e.
The acc u m ula tion of all this m at eri al seemed suffi eient to th e au thor to
su ppo r t a syste matic restud y 01' aU PeniciUia. As th e present syste m 01'
The colony 01' a Penicillium exhib its certa in ty pical and striking characte r
istics. T hese inc1ud e colour and colour changes; texture whi ch m ay be velvety
whe n th e stipes arise from th e su bs tratum, lanose whe n th e stipes 01' the conid io
Note . Shortly a fter complet ion of the prese nt study, the book "The G enus Penicillium and its
T eleom or ph ic Sta tes, Eupenicillium and Talaromyces' by John I. P itt (1979b) was published . Th ree
spec ies of Penicillium, P. erythromellis H ockin g, P. dendriticum, and P. loliense Pitt were describ ed in
it as new in the wo rld. These have bee n inc1 uded here in an Addend urn , where they a re
described a nd illust rated applying the sta ndard method s used throug hout the presen t work. O n
th e other hand , a nd as a matte r of conve nience, no acco unt had been m ade her e of Pitt' s new
class ificat ion ofthe gen us Penicillium. For further informa tion , the book itsel f(Pi tt 1979b) should
be consu lted .
phorcs ari sc from simple nc rial hyphnc, [unlculose wh en th e stipc arisc 1'1'0111
prostrat c Iascicles 01' acri al hyphae, uvfasciculatc wh cn th e stipcs ari sc 1'1'0111 crcct
lusciclcs 01' aerial hyphae or sy u nc ma ta . 111 sev era] spccics th c stipcs 01' the con i
. liophores are both monon cmatous and loos ely synnematou s, as in P. expansum .
In P. claviforme and in P. concentricum the synnemata are differentiated into a
d istin et stalk and an enlarged head composed of th e sporulating structures .
Another typical characteristic is the habit, which may be restricted to broadly
spread in g , a nd range from plane to de eply furrowed 01' wrinkled . The
suhstratu rn may 01' may not be dis coloured . Conspicuous drops 01' transpired
lluid a re charac te r istic of sorn e spec ies but not 01' othe rs . Odours may 01' m a y
1101 be produ ced, and if present m a y be charac te ristic 01' just 'mouldy ' .
Although th e gen eral appearance 01' su ch colon ies is influen ced b y the
n-l ative ab u n d a nce 01' conidial structures and scleroti a , whi ch may vary from
s rrain to strain , th e cu ltu ral charac teristics 01' a sp ecies seem to depend on the
use 01' a particular substratum .
C olon ies 01' most sp ecies and strains grow well on th e usu al Czapek' s
solu tion agar. Sorne sp eci es, however, are ch a rac ter ized b y a ver y pOOl'
dcvelopment on this medium. In m ost ca ses su ch restrict ed gro wth m ay b e
attributed to th e in ability 01' th ese strain s to synthe size the vit amins n ecessary
or to utili ze nitrate as a source 01' nitrogen . On th e othe r hand , malt-extract
agar usu all y su ppo rts good growth .
The co m position 01' the medium has a mark ed effec t on th e texture and
su rface ch a rac te r 01' colo n ies. On C za pek ' s aga r m edium the colon ies are
usu all y clos e textured , co m pa ra tively thi ck a nd sometimes va r ious ly furrowed
01' wrinkled . On malt-extract ag ar medium th e co!onies are u su ally loose
textured , co m paratively thin and plane, a nd sometimes a p pear zanat e .
CO LOU R OF C ON ID IA L AR EAS
C olony colour other th an shades 01' blue- green as well as colou r changes a re
ver y us eful in the id entification 01' strains a nd character iza tion 01' species.
Ev ery d escription 0 1' Penicillium em phasize s sorn e colo u r 0 1' se ries 0 1' colou rs
as charac te r ist ic 0 1' con id ial areas. When using seve ral cu ltu re m edi a , it is
necessary to sp ecify th e colou rs upon each by n ame , 0 1' numerical references to
sorne al' th e recognized colou r standards. Ridgway 's 'Colo u r St andards and
Nomenclature ' (191 2) h as been us ed by Raper a n d Thom ( 1949 ) . Much of the
Europe a n lit erature referred to Klinck sieck a nd V al ette ' s 'Code des Couleurs '
( 1908) . Sgu y ' s ' Code Universel des Couleurs ' ( 193 6) was publish ed in
Fr an ce . M ore recently , R a yn er (1970) published his ' Mycolog ical C olour
Chart'. Reference to suc h m anuals will undoubtedl y beco m e necess ary in th e
future since both Ridgway 's , and Klincksi eck and Valette 's works a re out al'
print a n d consequentl y no longer eas ily availa ble .
C olou r records show co ns ide rable variations 1'0 1' particul ar strains a s
int e rp ret ed by dilTe renl individu nls , 01' eve n by th e sa me person workin g a t
diff c rcnt times . For thi s rcaso n cita tion of co lo urs is nev c r a n a bsolu to gu ide. In
gene ral, how ev er, th e colo u rs produ ced by a give n strain upon a sta ndard ize d
su bs tratu m under standard cond itions, fall co nsiste n tly within a narrow range
of shades . H ence, th e cu ltu re medium must be spec ified if the co lou rs cited a re
to hav e real value . O n the other h and , colo u r photographic records 01' colonie s
represent a n excelle n t m ean s 1'01' di scardin g personal interpret ation 0 1' colou rs
an d h a ve been used by th e a u thor throughout th e present stu dy.
The ae r ial m ycel ium 0 1' th ese fun gi is ge ne rally colo u rless. Areas 01' ye llo w
a nd red h yphae co m monly a ppe a r in th e one -stage branch ed Penicilli a 0 1'
R ap er a n d Thom' s ( 1949) Biver ticillat a -symmetrica sec tio n. In th is case th e
colou r ing substance is largel y deposited as granu les on th e surface 0 1' th e cell
rathe r th an with in th e cell itself.
Su b merged m ycellium , on th e othe r hand , m ay sh ow di stincti ve colours
which are best a pp rec ia te d from below a n d a re routinc1y desi gnated as colou r
0 1' reverse. Di rty wh ite 01' dull buffs a re sometimes produ ced , but th e
com monest colours a re var ious shades 0 1' ye llo w, orange, red 0 1' purple . In
sorn e cases th e pi gment produced m a y diffus e into th e surrou nd ing agar
medium. T he di stincti ve a nd bri ght pigments a re parti cul arly ev ide n t in fresh
isolat es.
E X UDA T E AND ODOUR
T ite Peni cillia re presc nt a variabl e gro llp 01' moulds. Sectorial m utation s,
loss (JI' ahi lity lo produce spores and t he rnany variati on s whi ch ofte n acc u r,
nlles l lo th e cha ngability 01' th cse fun gi . T hese variations m ay devel ap as a
rcsu lt 01' progressive cha nges under ca ntin ued labaratary cultivatia n. When
I he cha nge cornrna nly rernains stablc thra ugh su bsc q ue nt su bc ultures it may
he rcgarde d as a true mutati on. When the change contin ues to sho w fur th er
chunges in th e same, 01' in sorne other directi on , it is usually regarded as a
vur iai io n .
Mut ants characte rized by colou r cha nges or striking nutriti on al deficien cies
sorne times isolat ed from nature . Seve ral strains have been observed to
dcvclop in a m anner typi cal of well- recognized spec ies upon a n enriche d
mcdiurn suc h as m alt extract aga r, but to gro w sparse ly and very atypically
Il!l0n Czape k's agar medium .
Areas of sterile , or esse nt ially sterile ove rg ro wth m ay a t times occur in
o rhc rw ise typi cal str a ins of almost a ny species of Penicillium. This overgr owth
ra n be isolat ed a nd cult iva ted separately and th en exhibits littl e if a ny of th e
r ha rac reristics of th e par ental strain.
;11'(:
Import ant fea tu res of th e st ipes include th eir len gth , septa tion, di ameter a nd
or igin in relati on to the su bs tratum and to eac h ot he r. Althou gh va ria tion in
stip e len gth u su ally occu rs in mo st cu ltures, th e majority of stipes are of a
defin ite ave rage len gth wh ich is characte ristic of th e spec ies . They are often
com paratively short, less th an 100 tut , espec ially whe n th ey arise from ae rial
hyphae a nd bea r sim ple penicilli . When they arise di rectly from th e basal felt
01' su bs tra tu m they are u su ally longer but seldom exceed 250 p.m in len gth .
When long , th ey are sometimes branch ed and ofte n beal' one- or two-stage
branched penicilli . T he wa lls m ay be smooth and th in or m ay be various ly
rou gh en ed , appea ring deli cately echinulate , gra n ula r or asperula te. In sorne
10
o
branched) . E, Typical
biverticillate-symmetric
penicillus, as seen in
Penici//ium variabile Sopp,
consisting of a stipe
terminating in a simple
vert icil of metulae, each 01
Latin ampulla = flask) of the ty pe show n in fig: 1.1 A and B. The one -stage
bra nchcd forms, R ap er and Thom's (1949) Biverticilla ta - sym metrica section,
p roduce phi alides that are us ually acerose (from the La tin acerosus = needleshaped ) like leaves of Pinus , as shown in fig: 1. 1 E . A few species prod uce
al most cylindrica l phi alides, lackin g a definite term inal na rrowing (see R ap er
an d T hom's ' M an ual of the Pen icill ia ' (1949) , page 49 , Fig. 12 A l a nd A2) .
T he phialide neck (the con id iu m -be aring tube of R aper a nd Thom 1949)
wa s tcr med collulum by Za leski (1927) , (from the La tin collulum = littl e neck) ,
it is usually long in sorne spec ies (see R ap er a nd T hom's Manual 1949, pa ge
47, Fig. 11 B) or sho rt, and is also of taxon omical im po rta nce .
o
branched). E, Typical
biverticillate-symm etric
penicillus, as seen in
Penicillium variabile Sopp,
cons isting of a stipe
term inating in a simple
verti cil of metulae , each 01
Latin amp ulla == flask) of the ty pe show n in fig: 1.1 A and B. T he one-s tage
bran ch ed forms, R aper and Thorns (1949) Bivert icilla ta- sym metr ica section,
p rodu ce ph iali des that are us ually acerose (from the Latin acerosus == needleshaped) like leaves of Pinus, as shown in fig: 1. 1 E. A few species produce
al most cylindrical phialides, lackin g a definite term inal narrowing (see R aper
and T ho m's ' Manual of the Pe nic illia' (1949), page 49, Fig. 12 A l and A2) .
T he phialide neck (the conidium-bearing tube of R aper a nd T ho m 1949)
was tcr med collulum by Zaleski (1927) , (from th e Lat in collulum == little neck),
it is usu ally long in sorne spe cies (see R aper a nd Thom ' s Manual 1949, page
47 , Fig. 11 B) or sho rt, an d is also of ta xono mical impor tance.
M I(TUJ.Al-:
Thc ICI'IIl metula (We sllillg 1911) is maint uincd fill' (he pcnuh imut c hrnn ch
wh ich bca rs the w ho rl 01' phialides . Thcy are charac te r ist icnlly borne in a
te rmi nal vcrticil on th e st ipc, or on it a nd cm o ne 0 1' more branch cs . Whe never
the wa lls a l' th e stipe a re smoo th, th ose of th e metul ae are also co ns iste n tly
smooth. W he n the walls 01' th e stipe are pi tted or ro ug h, th e walls 01' th e
m etulae may or may not be sim ilarly ro ughened .
BRA NCH E S
AII cells be tween m etulae a n d stipe s are refer red to as b ranc hes . 1'0 indicat e
th e ty pe 01' branching, th e ter m s pro posed by R aper a n d Thom ( 1949) a re
replaced by th e te rms simple (not branch ed = monoverticill at e), one-stage
branched (b ive rticilla te, as in P. citrinum or species 01' th e Biverticillat asym metrica sec tion), two-stage branched ( = Bive rti cill at a - symme tri ca , as in P.
italicum W chmer, var. italicum Sa mson, Stolk et H adl ok 0 1' P. expansum), or three
or more stage branched, as in P. griseoJulvum.
CON ID IA
Typ ically th ese cons ist 01' very h ard m a sses a l' pse u do pare nc hy mato us tissue
in which cell walls are ve ry thick. Among th e sim p le Penicilli a, R ap er a n d
Thom ' s Monoverticillata section , th e produ ction a l' ellip tica l to glob ose
sclerotia is regularly characte r istic al' m embers a l' th e P. thomii series. In P.
turbatum al' th e same series, small, soft m asses 01' thi ck-walled cells are usually
encou nte re d . These h ardly re presen t true sclerotia in the se nse al' P. thomii a n d
P. raistrickii, but ac tually th eir presence is st ill accepted as evi de nce al' a
1'('1111 ionship
'hapt cr 2
I hcy provide the maximum a mou nt of information from the fewest possible
lelll s in the minimum time. First must come the cho ice ofmedia : Thom (1910)
1I 1led potato agar and bean agar ; W estling (1911) used prune cxtract gclatine;
lliulIr ge (1923) a nd Zal eski (1927) gr ew their cu ltures on Raulin' s solu tion plus
c ln t ine 0 1' agar. Raper and Thom (1949) em ploye d three standard media:
( :zlIpck ' s a gar , stee p agar , and malt-extract agar. In more re cent studies stccp
If.l lll' has so metimes be en omitted because it is not widely a va ila ble . Abe (195 6)
IIIH:d , in ad d it ion , a Czapek's a ga r m edium with nitrite su b st itu ted for nitratc.
111 Il llld ies on Eupenicillium , Stolk and Scott (1967) added d escriptions of gr owth
1111 co ru -m eal ag ar. Pitt (1973) recommended that th e three basic media uscd
IIY R npcr a nd Thom (1949) for prep a ring co lon ia l a nd morphologieal
dl'llcl'ipli ons be reduced to two : Czap ek ' s yeas t-au tolysate agar (CYA) and
ur ult -cxtra ct a ga r (MEA) . In th e present work we adop te d th e foIlowing threc
Imll ic media , wh ose formula a re given below: Czapek's agar (CA), CYA and
M EA. AIl species cu r re n tly d escribed have been grown on th ese three standard
cu ltu re m edia. AH th e forms gro w well at room temperature (20 -25 C) with
d illusc d a ylight. Hundreds of Penicillium strains , including th e typ e stra ins
ohruined from the most important internation al cu ltu re co Ilections, have becn
x udi cd a nd com pared. The cult u res cite d in th e text by number, bearing th e
prcfix IJ FM , a re accession ed with our own fungu s cu ltu re coIlect ion .
S T i\ ND A R D I Z E D CULTU RE MEDIA
Czapek '5 agar medium h as the foIlow ing co m posit ion (Rape r and Thom 1949) :
N aN03
K 2 H P0 4
M gS0 4 7H 20
KCl
FeS04. 7H20
Su crose
Agar (Difeo)
Di still ed wat er
3 .0 g
. 1.0 g
. 0 .5 g
. 0. 5 g
. 0 .01 g
. 30 .0 g
. 20 .0 s
. up to 1000 m
.
.
.
300.0
50 .0
50 .0
1.0
g
g
g
g
This stock solution will keep ind efinite1y ; th e slight precipitate of ferric
hydroxide does not affect th e final medium.
.
.
.
.
.
1.0 g
10.0 mI
5 .0 g
30. 0 g
20 .0 g
up to 1000 mI
Malt extract agar has th e fol1owing com position (Raper a nd Thom 1949) :
Malt extract (Difco) . . . . . . . . . . . . . . . . . . . . . . . . . . ..
Glucose . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Bacto-peptone (Difco) . . . . . . . . . . . . . . . . . . . . . . . . . .
Agar (Difco) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Distilled water . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ..
20 .O g
20 .0 g
1.O g
20 .0 g
up to 1000 mI
The pH is not adj us ted a nd the medium is autoclaved for 15 min a t 121C
(15 lb overpressure) .
Media other th an C zap ek ' s agar were u sed on slide cultu res in certa in
instances wh en con id ia l development on this medium wa s poor or absent:
CYA, MEA and corn-meal ag ar. In su ch instances , wh en ever th ey were u sed ,
they are indicated in the tex t.
'om-meal agar (Di fco), T o rchydrat c th c mcd iu m , su spen d 17 g 01' Bacto r urn-mca l agar (Dif co) in 1000 mi 01' co ld d isti lled wat er and heat to boiling to
d l"Plolve th e mcdium co m plc tcly. Di stribute in tubes and autoclave for 15 min
1I I ~ I oC or 15 lb over pressure. Thc final pH of the medium wil1 be 6 .0 and is
nol udj us ted .
l U I.'I' U RE GROWTH AND INOCULATION MEDIA
1rcscriptions 01' colon ial ch a racte ris tics 01' th e va rio us species studied were
from th ree -spot in ocul at ed m edi a in Petri di sh es. The media rou tine ly
used were C A, CYA , a nd MEA .
For m icr omorphological studies slide preparations were m ade using a n
IlI" e OU S solu tio n 01' 0.01 % Tween 80 as th e m ounting fluid. Small fra gments
of a 7-day-old colony were torn up into ve ry small pi eces w ith ve ry fine
d issecting n eedl es (insect pins fixed on wooden h andles will do perfectly) ,
suspend ed in the abo ye m ounting fluid in test tubes a nd shake n for 2-3 min in
u vortex mi xer a t hi gh speed in orde r to wash ou t th e clou d 01' co n id ia, wh ich
othcrwise wo u ld obscu re the m icroscopi c obse rvat ion 01' th e penicilli.
I lla d e
I NC UBATI O N TI M E
1"01' colo ny m easu rement to b e 01' m ost value , a stan dard in cubation tim e is
r-sscntia l. The author h as chose n 14 d ays as stan dard for colony measu re men t
nnd colour pho tograp hs . H owever, becau se penicilli di sintegr at e with age,
rc nde ring m icroscopi c exam ination diffi cult , a sta n dard in cu b a tion period that
is as sho rt as possible is hi ghl y desirable for th at purpose. 7 da ys a t 25 C is
cons ide re d op timal for slid e cu ltu res.
S UDE CULTU RES
Con id ial structures were exami ne d gene rally on slide cu ltures al'ter 7 d a ys
incubat ion a t 25 C , while th e fu ngus is in a n ac tive state 01' gro wt h . T he
Fig. 2.1 . Equipment. A, Reichter 'Neopan' microscope (Austria) provided with a drawing
device or camera lucida Reichter, Model 21,391 . B, Low power 'Kyowa' binocular (Japan),
with zoom lens, extremely useful for making preliminary observations of colony
characteristics. C, 1 mi inoculation pipette with a closed and bent ball-pointed end.
--
Fig. 2.2 . Equipment. A, An 'exploded' view of Cole and Kendrick 's culture chamber, showing
the drilled and slotted slide, the agar medium, and the coverslips wh ich seal the upper and
lower surfaces. B, View of the Reiss microchamber, showing: (a) the slide, (b) strip of
adhesive paper , (e) side parts of the chamber, (d) the coverslip, and (e) the inoculum. C, An
'exploded' view of the drawing device: (1), Camera lucida . (2), Fixing screw to affix the
camera lucida to the microscope. (3), Ad justing ring for focusing . (4), Fixing screw, to affix
the camera lucida eye-piece. (5), Camera lucida prism's casing. (6), Screen for cutting off the
path of light through the camera lucida 's prism when the apparatus is not in use. D,
Schematic diagram showing the basis of the camera lucida theory.
Other techniques may be us ed 1'01' th e study 01' org an ization and ontoge ny 01'
ae rial str uc tu res . M any 01' them have been em ploye d 1'01' thi s study . In order to
ens u re aeri al gro wth, th e fun gu s is u su all y gro wn on slides on a thin film 01' an
appropriate su bstratu m. The conid iophores will gro w off the sides 01' th e
m edium in to th e surro u nding a ir and ca n thus be exam ine d (see V ernon 1931 ;
Riddell 1950 ; Anton y and W alk es 1962 ; C ochrane 1963; Zo be ri 196 7) . Cole
and K endrick ( 1968) described a sm all cult ure cha m ber 01' glass (fig. 2.2 , A)
whi ch all ows cultiva tion 01' th e fu ngus in suc h a m anner th at th e develop m en t
01' fu ngal struc tures ca n be contin uo u sly observe d and even film ed under hi gh
m agnifi cat ion . The constr uctio n 01' suc h a chambe r, however, requires great
per sonal skil!.
Consi de ring th at m any conidiopho res are gene ra lly negati vely geo tro pic
(Coch ran 1963), an eas ily producibl e and in exp en sive cult ure cha m ber was
described by R eiss (1970) (see fig . 2.2, B) , whi ch allows the study 01' the
development 01' re pro d uct ive fungal str uc tures in an undisturbed po siti on.
Su ch a cham be r has been used in our lab oratory with success . On a clean slide,
two small str ips 01' glass (the thi ckness 01' a slide) are glue d with nontoxic
mucila ge (i.e . U.H.U from Fisher, B hl , FRG ) at a dis ta nc e 01' 14 mm from
eac h othe r. This frame is ste rilized . A ste rile coverslip (18 x 18 mm) is placed
ayer the gap bet ween th e two glass strips a nd fixed to th em with two strips 01'
adhesive tap e . A small piece 01' a Petri di sh cultu re 01' th e fungu s is removed
under ste rile cond itio ns a nd placed on th e slide in fro nt 01' the spa ce bet ween
th e cover a nd th e slide . With a small and sterile strip 01' glass thi s in oculum is
pushed between th e slide and th e cove rslip . One sid e 01' th e op en cham be r is
closed with the strip 01' glass, th e othe r remaining ope n to ens ure a su fficient
supply 01' a ir.
T o enable negative geo tro pic gr owth 01' con id iophores, th e ino cul at ed
cham be r is put in a ve rtical position into a ste rile tr ou gh 1'0 1' slides , th e bot tom
and side s 01' whi ch are lin ed with sterile wetted filter papel'. U nde r th ese
cond itions rep roducti ve structures ca n grow upright int o th e air. The tr ou gh is
in cubat ed at a n appro pria te temperature (20 - 25C) under d iffuse light. Al'ter
varyi ng periods th e cham be r is removed and exam ine d under th e m icro scop e .
When th e mi croscop ical study has been fini sh ed , alco hol is dropped into th e
chambe r , th e coverslip is removed , cleaned and sterili zed , together with th e
lower part of the cha m ber. U se ofthe stro ng illuminati on necessary 1'01' m aking
mi crophot ogr aphs ca uses fine d rops 01' conde nsation occasionally to arise on
th e inner side 01' the cove rslip, but th ese evaporate sorne time afterwards .
111
'-' V ~
T his step is necessary to com plete th e dehyd rution proccss, lo avoid colla pse
01' fungal cells a11(1 sh ri nkugc 01' ccll wall lay ers. A Sumd ri-Put -S a uto matic
a ppa ratus, with a 15-min purgin g tim e was uscd by th e auth or , Bcfore coa ting
(the spec ime ns are shadow cas t with palladium - gold (40 - 60 ) in a vacuum
eva po rato r), th e sa m ples we re tak en from th e ca ps ules and fixed to a srnall (5
mm di am .) tin di sc with a n electroco nd uc tive silve r glue (Do lite) . The tin d iscs
may be ea sily m arked underneath for identificati on , and fin all y placed on a
sta ndard specimen stub by m eans of th e same silve r adhesive m ixture .
The con id ia were viewed a nd photogr aphed with a J EOL jSM-50A
sca nn ing e1ectron mi cr oscop e at an acc ele rating voltage of 15-20 kV.
CAMERA L U CIDA DRAWINGS
The m aj ori ty of typ e strains stud ied were obtaine d from th e Cent raalbu reau
voo r Sch im m elcultures (CBS), Baarn , The Ne the rla nds. O the r were obtained
from th e Ame rica n T yp e C ulture Collection (AT CC) , R ockville , M aryland ,
USA. A few stra ins were obta ine d from th e Com mo nwealth M ycological
In stitute (C M I), Kew , U K, a nd from the In stitute for Fermen tatio n (I FO),
O sak a , j apan . Additional strains of new or prev ious ly describ ed species were
secu re d fro m numerous fresh isolates. The latt er were chec ked both cult ura lly
a nd mi croscopically against th e type stra ins.
0 1'
pcnicillia
Miles occur in n utu re on decay ing plunt nuu eri al , on gra in , flour, chcc sc,
du st a m i so il pa rticles. T hosc infectiug lun gal cu ltures most co m mo nly bclon g
to suc h gen era as Tyroglyphus and Tarsonemus. They are most cas ily co n tro lled
by high standards of hygiene in laboratories whi ch hav e a screen in g procedure
1'01' in coming cu ltures a nd whi ch do not have to handle or ga n ic m at erial. In
laborat ories whi ch h a ve to handle fre sh plant m at erial , soil, b at ch es 01' grain 01'
m anufactured st u ffs, precautions must b e taken against mites, particul a rly in
humid c1imat es . W e found that storage 01' co n tam inate d m a terial in th e deep
freeze chest 1'01' 8 h is sufficient to kili all mites.
C HEM ICAL AN D PHYSI CA L B ARRI ERS AGAI NS T MIT E I N F E ST ATI O N
Q.)
a.
o
1
a.
ro
::J
c:
ro
E
Q.)
.....
..c:
lhap ter 3
Page
28
201
507
611
The Monoverticillata
( 1949) WAS T HE
M onov erticillata section. They included in it both th e perfect and imperfect
sp ecies in an a ggregat e of seri es a nd species bound together by th e a rbitrary
fact th at th e penicillu s was simple, i. e . it cons isted of a single clu ster of
phialides at th e tip of th e conid iopho re.
In th e present work , th e auth or m aintains th e Monoverticillat a section, but
in order to be in accordance with th e Internation al Code of Botanical
N omenclature (Sta fleu 1972) , al! species with perfect sta te s cons idered by
R aper a nd Thom (1949) in thi s sectio n have been exclude d fro m it.
The m ain characte ristic of thi s gro u p is th at th e penicilli co ns ist of a sing le
cluster or ve rticil of phialides at th e tip of th e stipe ; u su all y unbranched ; in
sorne forms irregul arly branched but with eac h branch terminating in a di stinct
and sep arate sim ple penicillus .
Few sp ecies are strictly simple , that is , produce stipes without branch es
below th e ve rtici l of phialides. In a sin gle se ries, design ated by R aper a nd
Thorn (1949) R amigen a , the stipe is nearl y always branch ed at various sep ta,
with the branches vary ing in len gth and usuall y di verging, but eac h branch
m aintaining th e a ppe ara nc e of a strictly simple penicillus .
The Penicillium thomii series of the Monoverticillata section is ch aracte r ized
by the production of true sclerotia, which in appearance and texture may
simulate cleistothecia .
Separation of th e simple forms from th e remainder of th e Penicillia is based
on examination of th e terminal verticil at th e tip of th e stipe. If there are no
branch es a t th e a pex of th e stipe , or if each branch maintains a fairl y definite
individuality , i.e . produces a simple penicillus, th e species is assi gn ed to th e
Monoverticillata section . However, no sharp line of sep aration can be drawn
between th e M onoverticillata and other species of R aper and Thom ' s ( 1949)
As ymmetrica section . In fact, th e different sections tr eated by th em are
intimately related and represent tr ansitions and different facets of a large and
variable group of moulds . As a matter of fact , com plete bridgings ca n be
observed between th e Monoverticillata section and th e Velutina sub -section of
th e As ymmetrica sec tion throu gh th e R amigen a series and Penicillium
corylophilum in the P. citrinum series . A similar transition from the Mono
verticillata to the Divaricata sub-section of th e Asymmetrica section occurs
through P. j ensenii.
T HE FIRST GROUP O F P ENICILLI UM TREATED BY R APER AND T HOM
:.W
29
prmlollll.
onloa volvety or nearly so; stipes arising mostly from the sub
trntum.
1' . Colonias ganerally spreading broadlv ,
111 . Conidia globose or subglobose
P. frequentans series o. oo 50
bb. Conidia elliptical
P. /ividum series oooo 70
' . Colo nias growing rather restrictedly, especially upon Czapek's
linar
P. imp/icatum series oooo 82
1.1 . Colonies appearing velvety or slightly lanate, but with the stipes
Sclerotia usually produced ; they are typically hard and gritty, consisting of
vCl'y hard sclerenchyrna-like rnasses of parenchyrnatous tissue with thick
wnllcd cells . In sorne species th ey consist of cornparatively soft rnasses of thi ck
wullcd ps eudoparenchyrnatous cells.
C olon ies usually spreading rapidly, developing abundant sclerotia which
o ftc n cha racter ize the colony appearance o In sorne species, howev er, colonies
~ l'Ow slowly and develop sclerotia only on certain substrates .
C on id ial structures abundant in sorne species , not in others ; with stipes
a l'ising directly frorn the substraturn, producing a velvety appearance, 01' frorn
trai ling vcgc ta tive hyph ac; va ry ing frorn short IIp to 300 - 400 J.'m in Icn gth ,
with walls smoo th 0 1' dclicatcly cchinulnto.
Pcni cill i str ic tly sim ple. H ow cv e r, so m c tim cs l!ley rlla y show a n occas io nal
bra nch , T he y a re spa rsc in sorne specics a m i stru ins . Phi alidcs a re typically
p a ralIel in co m pact clus te rs.
C onidia m ostl y elliptical to subglobose , srn ooth wall ed , usu alIy rel atively
smalI, 2 .5 -3 .5 .tm in di amete r , fo rmin g lon g cha ins often a d he re nt in loase
colu m n s.
Page
P. syriacum Baghdadi . . . .
b. Sclerotia soft, composed of pseudoparenchymatous thick-walled cells;
sclerotia produced only upon certain substrata
P. turbatum sub -series. . ..
1' . Colonies upon Czapek's agar developing rather restrictedly
aa. Colonies upon Czapek's agar not showing dull, dark purple
colours in reverse; growth somewhat restricted
P. turbatum Westling . . . .
bb. Colonies upon Czapek's agar showing dull, dark purple colours in
P. pusillum Smith . . . .
2'. Colonies upon Czapek's agar developing fairly rapidly
aa. Sclerotia produced upon all substrata
1". Colonies upon Czapek's agar showing brown colours in reverse;
sclerotia white, surrounded by brownish submerged hyphae
P. donkii Stolk . . ..
2". Colonies upon Czapek's agar not showing brown colours in
reverse; sclerotia white to cream coloured, not enveloped by
vegetative hyphae
P. indicum Sandhu et Sandhu . . . .
29
34
31
36
39
39
41
44
42
llricfly ch a ra ctc r ized , th e Pen icillium thom ii ser ies co m p r ises a gro u p of sim ple
Puu icilliu which regularly produ ce sclerotia . Raper a nd Thom ( 1949) sub
lvidcd lh e series, as a matter of co nve n ien ce, as follow s: (1 ) The series proper ,
in cludi ug a ll species produ cing a bu ndan t sclerot ia upon a ll su bs trata, whe rei n
1111: scle ro tia are ve ry h ard , brittle , a n d crush w ith difficulty ; (2) The P.
turbatu m sub-ser ies, in wh ich scle ro tia a re no t p rodu ced upon sorne su bs trata
( iud lld ing Czape k's agar), a n d are softer a nd re prese nt less co m pact m asses o f
ihick-wullcd ce lls .
'I'hrce spec ies ac tually co m prise the ser ies proper ; Penicillium thomii Maire is
l he oldcst, best kn own, a nd m ost a bu ndant species, appare ntly world -wide in
listribu tion. P. sclerotiorum van Beyma is less w ide ly di stributed a nd was
llI'i!{i na l1y isolated in J ava (Indonesia). It h a s been obtaine d fro m ot he r
su u rce s , rnostl y of tropical or igi no P. syriacum Ba ghdadi , isola ted from Syrian
d cscrt soi l, is characte r ized b y it s rather restricted growth upon a ll med ia, and
by thc p rod uct io n of b ro wn to blac k scle ro tia.
Fivc speci es ac tually comp rise the Penicillium turbatum sub-series, whic h are
ch uru c re r ized b y the p roduction of softer a nd easily crushed scle ro tia . P.
lurhutum W estling a n d P. pusillum Smith , bo th characterized by thei r rest ricted
g l'llw l!J upon Czapek's agar a nd P. donkii Sto lk, P. indicum Sand h u et Sandhu,
II UI! P. grancanariae R amr ez , M artn ez el Ferrer , characte r ized b y the ir fairly
I'lIpid growth upon Czapek's agar. Altho ugh the degree ofrelations h ip between
1hcsc spec ies and the members of the P. thomii series proper is q uestionable , it is
hcl icvcd , nevertheless, that th e y ca n be keyed here more sa tis factorily than
clscwhc re .
1, Pe nicillium thomii Maire : fig. 1, colou r plate 1, and SEM plate 1 (top :
right).
Penicil/ium thomi Maire - Bull. SOCo Hist. Nat. Afr. Nord. 8: 189-192. 1917. See
also Thom , U.S. Dep. Agric. Bur. Anim. Ind., Bull. 118: p. 78. 1910; also Thom,
'The Penicillia', p . 73 . 1930; also Raper and Thom, 'A Manual of the Penicillia',
p . 156. 1949.
Synonym ies
Penicil/ium crocicola Yamamo to apud Yamamoto, Maeda et Oyasu - Sci. Rep.
Hyogo Univ. Agric. Biol. Ser. 2: 28. 1956.
Penicil/ium paral/elosporum Sasaki - J. Fac. Agric. Hokkaido Univ. 49: 147. 1950.
Penicil/ium quercetorum Baghdadi - Nov. Sist. Niz. Rast. 7 : 110. 1968.
Penicil/ium thomii Maire, varo flavescens Abe - J . Gen. Appl. Microbio!. Tokyo .
2: 51. 1956.
nly (colou r piat e 1, A) , and ofte n failin g to devclop sclerotia, es pcc ially in
trui ns lon g m aint aincd in artifici al cult u res ; c xud a tc clca r . a bu ndantly
pn ulu ced in so rne strains, not in others; odour aint , sugge sting mushrooms ;
l't:V CI'SC (colour plat e 1, a) in pal e yellow to pinkish shades ; stipes 0 1' th e
r n uid iopho res a r ising primaril y frorn the substratum a n d from interlacing
1l:l'ial hyphae , seld orn branched , with walls delicately ec hi n u la te (fig. 1, A) ,
variable in length up to 300-400 J.l.m by 3.0-3 .5 J.l.m in diamet er, with a p ices
Hlightly c nla rge d in a ves icle abou t 4.0- 5. 0 J.l.m in di amet er; penicilli strictl y
ximplo, bea rin g co n id ial chains usu all y in loose colu m ns up to 150 J.l.m 0 1' more
in lcn gth (fig. 1, C); phialides m ostl y parallel , in cro wde d clu sters, 8 -12 in th e
vcrticil, usu all y 8- 13 J.l.m by 2.5- 4.0 um , with a somewhat n arrowed , sho rt
ucck (lig. 1, A) ; con id ia elliptical to subglobose (fig. 1, B, a nd SEM plate 1,
lop : right) , m ostl y 3. 0-3 .5 J.l.m in lon g axis, app arently smooth wall ed when
oh scrved under th e light mi cr oscop e but rough wall ed when observe d under
Ih e S EM.
Co lonies on CYA spreading broadly , a tt ain in g a di amet er 0 1' 45- 65 mm in
1,1 d ays at room temper ature (20- 25 OC ) (colour plat e 1, B, a nd b ), d eepl y
rudiately furrowed , ve lvety in m ost strains but with surface a ppearing loosely
l.u uu e in sorne isolat es; u su all y h eavily sp oring throu gh out , in yellow- green
shndes (S guy, pl at e X X , 299, 300), becoming dull gray in age, bu t remaining
wliite in sorne very poorly sp oring stra ins; margin irregularly dissect ed ; odo ur
l.uk in g; ex u date a bu ndan t as a m be r-colou re d drop s; a m be r-co loured pi gment
c1 iflusing into th e su r ro u nd ing agar; scle ro tia as described a boye, ab u ndan tly
produced in sorne strains , not in othe rs (colour pl at e 1, B) , commo nly obsc u re d
hy ove rly ing h yphae and co n id ia l structu res, in othe r cases dom inat ing th e
rulony ; penicilli as described aboye; reverse in pale cin n a mon shades (colou r
pla tc 1, b ) .
Colon ies on MEA spread ing b ro adly (colour pIa te 1, C a nd c), attain ing a
c1 ia met er 01' 50- 55 mm in 14 d ays a t room temperature, pl ane , zo nate, h ea vier
sporing than upon C A, th in m a r gin wi th su b merge d m yceli a exten d ing 5 mm
(JI' more beyond th e aerial growt h;
usu all y producin g abundant sclerotia,
lorrning in so rne strains a dense , con tinuous layer adjacen t to the su bstratu m,
lending th e colo ny a n orange- p in k colour interrupted only by a reas 01' h ea vy
ro nid ia l devel opment ; penicilli as described aboye but with th e stipes 01' th e
ro nid iopho re more cons p icuo us ly a nd closely ec h in u la te.
Spec ies d escription is ce n tered upon th e typ e strain CBS 38 1.48 = C M I
~(),02 7 = ATCC 10,506 = NR RL 1640 = QM 8, 002 = IJ FM 5017, isola ted fro m th e air
in the U S by K .B. R ap er , a nd fro m m any othe r strains isolat ed b y th e au thor.
T he species is ab u n dan t a nd world-wide in di stribution . It is especially
rornrnon in soil. When fir st isola te d, strains almost in va ri ably p ro duce
ab u ndan t p ink scler otia, but under con tinu ous laborat ory cul tivatio n th ey tend
to become in cr easingly co n id ial. Strains m aintained 1'0 1' long period s in
.ollcctio ns o tcn lose th c ir abilily lo produ ce thesc churac tcristic stru cturcs.
M cmbe rs of th c specics a re sul>jcct lo co nside rab le variu tin u in cu lt ure , a nd th c
sm all co lo n ics m uy co n ra in sccto rs th at a re ciihc r p rcdominnutly co n id ia l 0 1'
sclero tial. In addition to thesc vari ations , occa siona l st rn ins SllOW sclerotia less
dcliniteIy pink in coIou r , and produ ce smallcr co nid ia l st ru ctu rcs arising as
sho rt branchcs from int crl acin g ae riaI hyph ae , su ggestive 01' th e P. decumbens
seri es . But sclerotia have never be cn reportcd for an y of th e spc cies th at
comprisc th at seri es.
The sclerotia of P. thomii M aire are borne singly and are naked , 0 1' nearl y so .
The actual tenden cy is to regard as representative of P. thomii M aire all strain s
with sim ple penicilli producin g hard , brittIe , pink sclerotia .
~ 8 0~0O
O
0 0
lw..L.1.LwJ
10 jsrn
O
00 0
0 0 0
O
00 ()
0
()0 0 O
"
,.:"1)
L--I
~~o}Jm
:'~ ['
"J.. -1
~ ..\
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K.
Penicillium sclerotiorum van Beyma - Zentbl. Bakt. Parasitenk. Inf. Hyg . Abt. 11. 96:
416-419. 1937; See also Raper and Thom, 'A Manual of the Penicillia', pp.
160-163. 1949.
Rap cr and T hom's (1949) or iginal description is included below , sin ce thi s
ndc q uate ly presents th c species in qu estion wh en recently isolat ed. The typ c
sI rain 1\TCC 10.494 has lost some of its original cu ltural charac teristics sin ce
I he tim e of its isolation in 1936 by Professor Boedijn in Buiten zorg , J av a,
particul arl y th e ca pacity of producin g sclero tia upon all substrata .
Raper a nd Thom ' s original description is a s follows:
' Colonies on Czapek's agar growing somewhat restrictedly, attaining a diameter
of 20-35 mm in 10-12 days at room temperature , variable in colour and texture
depending upon the relative abundance of sclerotia , vegetative hyphae, and
conidial structures; varying from essentially conidial presenting a velvety
appearance, through more or fess floccose from the development of abundant
vegetative mycelium, to predominantly sclerotial with limited development of
fruiting struetures or of aerial vegetative hyphae, frequently showing these different
aspects as sectors in the same colony; conidial structures Iimited or abundant, at
first arising primarily from the substratum and en masse producing areas near
artemisia green, in age often developing from aerial hyphae over the entire colony
surface; vegetative mycelium in orange-red shades; sclerotia uncoloured or nearly
so, typically borne in fairly definite c1usters surrounded by envelopes of sterile
encrust ed hyphae in bright orange-red shades near scarlet to Brazil red, usually
occurring in limited sectors but sometimes characterizing the entire colony when
young; exudate colourless or nearly so , abundantly produced in areas of heavy
sclerotial or mycelial development, lacking or Iimited 'in predominantly conidial
areas; odour slight, suggesting mushrooms; reverse in yellow to orange-red shades,
not diffusing into the surrounding agar; penicilli strictly monoverticillate, bearing
conidia in parallel chains forming loose columns up to 150-200 p.m in length by
15-20 JLm wide; conidiophores arising from the substratum or from aerial hyphae,
seldom branched, smooth-walled, up to 200-300 p.m in length by 3.0-3.5 JLm,
enlarging in terminal areas to vesicular apices 5.0-7.0 JLm in diameter; sterigmata
parallel, in crowded c1usters of 12 or more, mostly 8-10 p.m by about 2.0 p.m, with
conidium-bearing tips somewhat narrowed; conidia elliptical, about 2.5-3 .0 JLm by
2.0-2.5 p.m, smooth -walled.
Conidia on malt agar spreading, up to 55 mm in 10-12 days at room temperature,
thin, usually heavy sporing, with eonidial areas velvety and light blue-green ;
sclerotia abundantly produced, in limited c1usters projecting through the eonidial
layers and appearing as bright red islands; penieilli as deseribed above but with
conidiophore walls delieately roughened.'
.ha ructcristics lo th e a bo ve doscript ion , cxce pt 1'0 1' th e abs cnce 0 1' scleroti a a nd
thc produ ction ofycllow-colourc d pigm cnt d illus ing into th e surroundin g agar.
Colo n ies UpOll C YA (colour plat c 2 , B and h) attainin g a diamct er 01' 40 mm
at room tempcrature (20 - 25 OC ), co n sisting 01' a closc- tc x tu rc d basal felt,
strongly and deeply radiately furrowed, with the centre sligh tly rais ed a nd
irregularly fold ed in a cra tcrifo r m p attern ; ve getaiive m ycelium brightly
colou re d in cin nabar inous shades influencing greatly th e co lo ny a p pearance ;
hea vil y spor ing in limited sect ors in artemisia gree n shades (S guy , XXX ,
438) with co nid ia l a reas appearing velv et y ; thin m argin surro u n de d b y a fringe
01' su b merged hyphae ex ten d ing a bo u t 3 mm beyond th e ae ria l gro w th;
exudate ab u ndan t , as ye llow -o range d rops ; odou r fa in t, ' mou ldy'; ye llo w orange-co lo u re d pi gment diffusing into th e su rro u nd ing agar; co lony reverse
in deep oran ge shades (colour pl at e 2 , b) . Scl erotia n ot observe d .
C olonies upon MEA (colour pi a te 2, C and c) growing restrict edly , att a in ing
a d iamet er 01' a bou t 20 mm in 14 days a t ro om temperature , pl ane , velvety,
heavil y spo r ing as aboye ; ex u date ab u ndan t; odou r ' m o uldy'; undulating
m argin largel y su b merged, ex te nd ing abou t 5 mm b eyond th e ae r ia l gro w th;
ye llo w-co lou red p igment diffu sing into th e surrounding agar, o the rw ise, as
describ ed a b oye . Sclerotia n ot observed .
Penicillium sclerotiorum is charac te r ize d particularly by its prominent scle ro tia .
As not ed by van Beyma ( 1937), th es e a re essen tially colo urless, 01't en
somewh a t a n gu la r in p attern , ranging from 200 to 400 tm in di amet er. In
recent isolates th ey m ostl y occur in clos ely com pactc d, sm a ll clusters a nd are
co n tained within a n e nve lope 01' sterile h yphae whose walls a re studded b y a
b right orange- re d pi gmented su bs tance which was isolated and in te ns ively
studied b y C urtin a n d R eill y ( 1940) a n d desi gn at ed scle ro tio r ine, after th e
species name . They a re very h ard , 'sto ne-like ' a nd are co m po sed 01' thi ckwall ed scle re nc hy ma-like ce lls 10-1 5 tm in di amet er.
Sp ecies d escription is ce n tere d upon th e typ e strain AT CC 10,494 = C BS
287. 36 = NR R L 2,074 = C M I 40 ,569 = IFa 6,105 = QM 1,938 = IJ FM 7241 , isolated
from th e a ir b y Prof. Bo edijn , in Buitenzorg , J a va, Indone sia , in 1936 .
The species is less abundant th an Penicillium thomii M aire . It ha s been
rep orted .from soil in south Africa , from leal' litter in the Congo, and from
det eriorating military eq u ip men t in tropical and su b trop ical regions .
:P
a BO 0 0
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100pm
('2 0 - 25 OC ), close -tex tured, to ugh , with surface appearing velve ty, dee ply
radi ately furrowed , slightly rai sed and fold ed in colony centre, at first white ,
with eve n m argin becoming dark blue-gray (Sguy, PI. X X XIII , 492) at
matu ri ty of co nid ial structu res; th in m argin wit h submerged hyp hae ext end ing
about 2 m m beyond the aerial growth ; odo ur lacking ; ex udate a bundant, in
large yellow d ro ps; bright yellow pigment di ffu sin g into the surrou nd ing agar;
colony reverse (colour plate 3, a) in ligh t brown shades . W h ile abu ndant
producti on of b row n to bl ack scle rotia was re ported in Bagh dadi ' s original
di agn osis of the spec ies, at no time , however , ha ve we obse rve d any ev ide nce of
suc h st ruc tures in th e type strain studied. This is probably du e to the long
maint en ance in artificial cultu re since the tim e of its isolation. The stipes of th e
conid iophore a rise pri marily from the substratum or from aerial hyphae (fig . 3,
C) , eithe r with walls smooth or fine ly echi n ulate (fig . 3, A), u p to 300 11m in
lcn gth by 2.0-3 .0 11m in diameter, with ap ices enla rge d in a ves icle up to
:>. 5 11m in di am eter. Con idial structures limi ted in number , not influencing the
colony appearance; pen icilli strictly sim ple , with phialides aggregated in
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compact cluste rs o f 4-8 pcr verticil, 7.0 -1 1.0 .UII by 3.0 -4.0 .o n , terrninated
in short narrowcd neck (Iig. :~, A) ; co n id ia globoso lo slightly clliptical,
3.0- 4. p.m in di amet er, deli cately echinu late to nca rly smooth wa lled when
obs erved under th e Iigh t mi cr oscope but co ns picuo us ly roughen ed wh en
observed under th e SEM (SEM plate 1, ce n tre : left); conid ial cha ins forming
loosely parallel colu m ns (fig . 3, C) .
C olonies on CYA (colour plat e 3 , B and b) spreadin g broadly , attaining a
di amet er of 40-45 mm in 14 d ays at room temperature (20- 25 OC ), sim ilar in
texture to the abo ve description ; eve n margin with a fringe of su b m erged
hyphae ex te nd ing 2-3 mm bey ond th e aerial growth , deepl y and radiately
furrowed , with ce ntre fold ed and depressed in a crate riform pattern; colon y
surface at fir st white to yellowish, with m argin becoming dark, lead gray with
age; spa rsely sporing in blui sh-violet sh ades (S guy, PI. XL , 593, 594); od ou r
lacking; ex ud a te a bu ndan tly produced in bri ght yellow drops ; bright yellow
pigm ent diffusing into th e su r ro u nd ing a ga r; colon y reverse (col ou r plat e 3, b)
in brown shades; th e stipe s of th e conid iopho re arise from the substratum and
from tr ailing ae rial hyphae as a boye; scIcro tia have not been obse rve d .
C oloni es on M EA (colou r plat e 3, C and c) spreading broadl y a ttai n ing a
diameter of 45-50 mm in 14 d ays at ro om temperature (20 -25 OC ) , plane or
with sha llow furrow s; colou rless a nd thin m argin , with a fringe of sub me rged
hyph ae ex tc nd ing a bo u t 2 mm beyond th e aerial gro wth; colony surface
gradually rais ed tow ards th e ce n tre; ce n tral and su bce ntra l a reas cove re d by an
over gr owth of whi te ae rial hyphae lendin g a sublan at e a p peara nce to th e
colony ; spo ring areas coloured in gray-green (Sguy , PI. XXIX, 430) to pale
blu e-gray shades (Sguy , PI. XXX , 449) , m ore or less zo nate, at cen tral and
subcentral a reas; odo u r lacking ; ex udate not ob served ; no colou re d pigment
diffusin g in to the surro u nd ing agar; scIerotia hav e not been obse rve d; colon y
reverse (colour plat e 3 , c) in pale yellow shades . Pen icilli as described above .
Sp ecies d escription co nce rn ing co nid ial structu res a nd colonial
characte ristics is ce nte re d upon th e typ e stra in C BS 418 .69 = BKM
F - 1,071 = lJ FM 5043 , isolated from Syri an desert soil by Ch . Baghdadi in
O ctob er 1963.
Haghd ad i' s original description of th e spec ies follow s, since thi s ade quate ly
presents th e species in question :
' Colo nies on CA atta ining a diameter of 20-25 mm in 10 days, velvety , radiately
furrowed but not deeply, irregularly folded, with margin enti re; colony surface at
first wh ite , assuming grayish colo urs, finally leading to gray shades in age ; colony
reverse in yellowish-brown shades; yellow pigment diffusing into the surrounding
agar; exudate abundant , colourless ; odour faint , suggesting m ushrooms; sclerotia
abundantly produced , variously shaped , spherical , ovoid , elliptical, brown to black
coloured , from 50 to 65 .m up to 150 .m in diameter . Conidiophores arising directly
frorn the substrat um or f rom aerial hyphae, measu ring from 30 to 300 .m in length
by 2.6-3.0 ILm in dlamet er, with wall s spinulose and aplces enlarg od in a vosicle
3.5- 6.0ILm in diam eter. Penicilli strictly simple, somet imes aggregat ed in clusters of
2-3; phlalides, bottle-shaped, 6.5-9.0 JlITI by 2.5-3.0 JlITI in clustsrs of 5-8 per
verticil; conidia globose to slightly elliptical, with walls echinulate , 3.0-3.5 11m in
diameter; conidial chains up to 65-160 11m in length .
Colonies upon MEA attaining a diameter of 25 mm in 10 days, close textured,
with surface asperulate, yellow-white in colour; exudate lacking; reverse in yellow
shades with similarly coloured pigment diffusing into the surrounding agar.
Colonies upon corn meal agar attaining a diameter of 30 mm in 10 days , buckled
and wrinkled, velvety, partially lanate, in yellow-white shades; exudate colourless ;
reverse in reddish shades. Th is fungus ca~~ot be related to any known species. It
was isolated from soil near Berza and Kabun , in the Damascus area and near Es
Suveida (Svria) in October 1963. The type culture is maintained with the Damascus
University Fungus Collection (Svrial and at the Lomonossov University Fungus
Collection, Moscow, (USSR):
(Translated from the Russian by Prof. L. Vlas).
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Pen;c;//;um turbetum Westllng - Arklv Bot. 11: 128-130. 1911. See also Thom, 'The
Penicillia', p. 207. 1930; also Raper and Thorn, 'A Manual of the Penicillia', pp.
166-167. 1949.
d ilfer mnrkedly 1'1'0111 those 0 1' Penicillium thomii M alre in th ut thcy are
.o m pnrutivcly son, crush eas ily , a nd are com poscd 0 1' pa rcnchyma-Iike cells
with walls mod crat ely thi ckcn ed. T hc sclero tia a re adj accnt to the subs tra tum
a nd parti ally obs curcd by overlay ing co nidia l str uc tu res, attaining a d iam eter
up to 150 un (fig. 4, O) , colourless to ycIlowish, com posc d 01' polyhcdral ceIls
10-1:) /lm in diameter, with waIls 1.0-2 .0/lm thi ck ; penicilli as descr ibed
ab oye.
Species description is cente red up on the typ e strain C BS 383 .48 = C M I
39,7 38 = ATC C 9 ,78 2 = NRRL 757 = I FO 7,762 = Q M 1,941 = IJ FM 5016, isolat ed by
W estling probabl y from soil, in 1911.
c,l
5. Penicillium pusillum Smith: fig. 5, colour plat e 5, and SEM plat e 1
(bottom : left ).
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Penicillium pusillum Smith - Trans . .Brit. Mycol. Soco 22: 254-255. 1939. See also
Raper and Thom, 'A Manual of the Penicillia'. pp . 167-168. 1949
t\'~J-oo
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C olon ies on C zapek's agar (co lour plate 6, A and a) gro wing fa irly rapidly ,
attaini ng a d ia me ter of 40- 50 mm in 14 days at room tem pe ratu re
(20-25 OC), plane bu t somewhat raised and flu ffy in the ce ntre, which is
cove re d by tufts of ste rile hyphae , giving a cottony appearance to th e colony;
wh ite at first , becoming grayish green in heavily sporing a reas, wi th m argin
irregul arl y di ssected from the uneven growth of the vegetative myccl iu m;
exudate abun dant , colou rless; odou r lacking or in definite ; sclerotia abu ndant ly
produced , lending a granula r appearance to the colony su rface, globose to
su bg lobose (fig . 6, D ), white to cream colou red, covering th e entire colony
su rface, soft , easily crushed, formed of thick-walled pseudop arenchyrna-like
cells, not envelope d by h yphae , m ostly 60- 150 .tm in th e long ax is. Penicilli
strictly sim ple (fig . 6, A), with the stipes of the conid iopho res a ris ing prim arily
from ae rial hyphae , short, smooth-walled, ranging fro m 17.0 to 40 .0 .tm by
1.5- 2.5 tut , with apices en la rged in a vesicle 3.5 to 6. 0 tui: in d iame ter;
7. Penicillium donkii Stolk: fig. 7, colour plate 7, and SEM plate 2 (to p:
left) .
Penicil/ium donkii Stolk - Persoonia . 7: 333-337. 1973.
~.
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Iate r sho wing dark brown shades (Sguy, PI. XLVII , 69 1); d iffus ing pigment
into the su rro u nd ing agar dark brown; soft, white, setose sclero tia- like
struc tu res usu ally produced near th e agar su rface, globose to su bg lobose (lig .
7, D), mostly 60-150 /lm in di ameter , hyaline when yo ung, but soon
bccoming brown ; pseudop aren chym at ou s walls co nsis ting of com paratively
sma ll irregu la r cells, 2-3 /lm in d iame te r, bearing n umer ous, septa te, hyaline ,
rad iating , stra ight hyphae , up to 300 /lm in len gth , completely obsc u ring th e
hrown in ne r bodies, giving a white appearance to th e struc tu re. H ea vily
spor ing in light grey -gree n shades (Sg uy, PI. XXV 375); penicilli stri ctl y
sim ple (fig . 7, A) ; stipes of th e con id iopho re s usuall y aris ing fro m th e bas al felt
a nd fro m aeri al veget at ive hyph ae, developing someti m es in marg inal a reas
lrorn subme rge d hyphae , septat e , va riable in len ght , ra ng ing from 20- 300 /lm
by 1.5- 3.0 tui: in di ameter , smooth -walled or nearl y so, with apices enla rged
in a ves icle 4.0-5.0 /lm in di ameter; phialides in cro wde d who rls up to 10-1 2
per verticil, cons isting of a cylindrical, bottle- shaped base tapering abru ptly to
a sho rt neck , 7.0- 10 /lm by 2. 0-3 .0 /lm ; conid ia su bglobose to ellipsoidal,
2.0-:U l
jllll by 1.5- 2.0 jllll , smooth when obse rved und er the light
ruicroscop e ( fi~ . 7, B) 01' dcl icat cly ro ughc nc d wh cn o1JS CI'V C<! und cr th c S EM
(SEM plat e 2 , top : lcft }; co n id ia l cha ins formin g wcll -d cfin cd , so rne timcs
sligh tly twi sted colum ns up l o 200 jlllJ lon g (lig. 7, C) .
Colo n ies on C YA (co lour plat e 7, B a nd b) sprcadi ng b road ly a nd rapidly ,
attain ing a di amet er o f 50-55 mm in 14 d ays at roo m temperature , co ns isting
of a loose-textu red felt , velvety in a ppearance, poorl y sporing throu ghout in
light pinkish shade s; strongly and deepl y radi ately furrowed , with ce n tre
depressed and irregularl y folded; thin m argin with su b merged hyph ae
exte nd ing 3- 4 m m beyond th e ae rial gro wt h; ex udate ab u ndan tly prod uced in
dark sien na shades (Sguy, PI. X.tI, 172); sim ila rly colou re d pigm ent d iffus ing
into th e su r rou nd ing agar; odo ur lacking ; reverse (colou r pl at e 7, b) in d ark
reddish b row n sha des; sclerotium-like bodi es not observed up on thi s
su bs tratu m. Penicilli as described a boye.
Colon ies up on MEA (co lou r plat e 7, C a nd c) sp read ing b ro adl y , attaining a
di amet er o f 50-60 mm in 14 d ays at ro om te m pe ra ture (20-25 OC ), cons isti ng
of a loose-textured basal felt, poorl y sporing th rou gh ou t in light gray -green
shades (Sguy, PI. XXVII , 400), with a b u ndan tly p rod uce d sclerotiu m -like,
white, setose bodi es affecting the colo n y with a gr anul ar a ppeara nce ; thin
marg in with su b me rge d h yph ae extend ing 5 0 1' more mm beyon d th e ae rial
growth; odo ur a nd ex udate lackin g; colo ny rever se (co lour pla te 7, c) in very
d ark , almost bl ack gree n shades; gree n coloured pi gm en t diffu sin g slowly into
the su rro u nding agar. Penicilli as d escribed aboye .
Species desc rip tio n is ce n tered up on th e type strain CBS 188.72 = C M !
197 ,489 = !J F M 5023, isolat ed by L. K . liver fro m ara ble Alas ka n so il.
10 urn
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fR.
fj
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plat e 8 , a) in ligh t cream shades; scle ro tia -like struc tures have not b een
ob served upon th is su bs tratum. P enicilli strictly sim ple o r ncarly so, sho wing
scldorn a n occa sional branch (fig . 8 , A) ; stipe s of th c co ni diopho res sho rt ,
usu all y up to 100-1 50 tm in length by 3.0-4. 0 tm in di amet er , a r ising
di rectl y fro m th e substratum, with walls r anging fro m d eli cat ely to coarscly
ro ug hened but scldom smooth , with ap ices e nla rg ed in a vesicle up to 7 tm in
di amet cr; phialides formin g cro wde d clus te rs of 10-12 pcr ve rticil, m ostl y
6.0- 13 tm by 3 .0 -5 .0 iutv , co m monly producin g ch a in s of co n id ia in fairly
co rnpa ct co lu m ns up to 400 tm long (fi g . 8, C) ; co nidia clli ptica l to
su bglobose, mostl y 3.0 - 4. 0 tm by 2.6- 3 .0 tui , ve ry delicat ely rou gh ened
whe n obscrvcd under th e light mi croscope (fig . 8 , B) , but showing a
ce rebr ifo rm pattcrn whe n observed with th e S EM (SE M plate 2, top : right) .
Colo n ies on C Y A (co lour piate 8, B a nd b) sp read ing b ro adly, attai ning a
diamct er of 45- 50 mm in 14 d a ys a t room tempcrature (20 -2 5 OC ), velvety ,
stro ng ly radiatel y fur rowed a n d irregularly folded in cen tra l area, very poorly
spor in g , m ostl y in cen tral arcas in ligh t gray-gree n shades; rc mainder n onspo r ing areas re main in g white ; cv en m argin with submerged hypha e
sxtcnding about 1 mm beyond the ncriul growth; odour und exudare lacking;
dark a m be r colou rcd pigm cnt dilfusin g into thc su rro u nd ing agur: colony
reverse (colou r plat o a, b) showin g cin namo n shades. Pen icil1 i as described
aboye. Scle rot ia ha ve not been obse rvcd .
Colon ies on malt ex tract agar (co lour plat e 8, C and e) spread ing bro adl y ,
at taining a di ameter al' 65 -70 mm in 14 days a l room tem peratu re
(20- 25 OC ), consisting 01' a rather loose-textured basal feIt , za nate, plane ,
heavily spo ring throu ghou t in defin ite gree n shades (Sguy , PI. XXV II , 403);
producing few, scattered, soft sclerotia-like bo dies in ora ng e shades , with wa lls
formed 0 1' thick pseudoparenc hymatous po lyhedral cells (fig . 8, D) . Penicilli as
described aboye .
~;
Penicillium grancanariae app ears most cIosely related with species al' th e P.
thomii series, in the P. turbatum sub -series, on account 0 1' th e presen ce al' soft
sclerotia- like bodies up on sorne subst rates (MEA in the presen t cas e), a l' the
rapid growth upon most med ia and a l' the strictly simple pen icilli.
Sp ecies descr ipti on is centered upon the typ e strain IJ F M 3745 = C BS
68 7.77 = ATC C 38, 668, isola ted from the air 0 1' Las Palmas de Gran C anaria,
Canary Islands, Spain by D I' R . Ruano-T ravieso , in July 1976.
The type culture N. 194 Is depositad at the Department of Cryptogamy, Univerltv M.V. Lomonossov, Moscow, USSR, and at tha Institute of Pomology and
Ampelography, Laboratory of Microbiology of Kazachstan, Alma-Ata region.'
(Translated from the Russian by Prof. L. Vilasl,
Iace wrinkled, zonate, velvety, margin at first white, then becoming dirty green, with
cont re grayish; exudate colourless; colony reverse brown. Sclerotia are abundantly
produced in colony centre, variable in size and shape, giving gray colours to the
colony surface , elliptical, ranging from 300 to 430 JUTl by 150JUTl, or globose, 130JUTl
in diameter, brown coloured . The conidiophores arising directly from the substrat um , smooth walled, 500 JUTl by 3.0-3.5 JUTl, usually terminating in simple
penicilli, although an occasional branch may be encountered, measuring 14-18JUTl
by 3.5 JUTl. Phialides in number of 4-6 per verticil, mostly 7.0-8.0 JUTl by 3.5 JUTl.
Conidial chains forming divergent columns. Conidia smooth walled, at first ellip tical, 2.5 JUTl by 3.0 JUTl, becoming later globose, 3.0 JUTl in diameter.
Colonies upon MEA attaining a diameter of 20 mm in 10 days, close textured,
velvety, somewhat lanate in zones, wrinkled, in pale cinnamon shades with white
spots; exudate lacking; colony reverse yellowish.
Colonies on corn mear agar attaining a diameter of 30 mm, strongly folded,
velvety, in cinnamon shades; exudate hyaline, colourless, colony reverse in
cinnamon shades. No closely related species are known.
The type species has been isolated from Syrian desert soil near Es-Suveida. Other
isolates have been recovered near Irna, Damascus region, Syria, from meadow soil,
forest soil, from gray chestnut, from Quercus forest soil, at 5 cm of depth, in
August 1964.
The type culture N 619 has been deposited with the fungus culture collection of
the M.V. Lomonossov University, Moscow, USSR, and with the fungus culture
collection of the University of Syria at Damascus.'
(Translated by Prof. L. Vilas from the Russianl.
Colonies grow ing ra p idly, usu ally sprcad ing broad ly, with a velve ty
appearance or with colony su rface slig h tly lan at c.
The stipes of the conid iop hores, scldom branch ed , smooth wall ed to
defini tely rou gh en ed , a rising di rectly from the basal felt or from trailing
hyphae .
Peni cilli str ictly sim ple, com paratively large , with phiali des crowded in
compact who rls, a nd with con id ial chains forming u su all y loose or com pact
~i;
columns .
Conid ia globose to su bglobose , seldo m ellipt ica l, ranging fro m 2 .5 to 5.0 utt:
or m ore in di ameter, smooth wa lled to coa rsely ro ug hened, dcp end in g u pon
the species.
Key to th e species
1. Colonies velvety or nearly so, with stipes of the conidiophores arising
mostly from the substratum . Colonies usually spreading broadly on most
media. Conidia globose to subglobose.
P. frequentans series
a. Colon ies upon CA strictly velvety, close textured .
1'. Colony reverse usually in orange -brown to redd ish-purple shades.
aa. Conidia mostly 2.5-3.5 tm, with walls thin, smooth or finely
roughened.
P. frequentans Westling
bb. Conidia mostly 4.0-5.0 tm, with walls heavy, dark green, and
coarsely roughened.
P. purpurescens (Soppl Raper et Thom
2'. Colony reverse colourless or nearly so.
aa. Colonies developing conspicuous stolon-Iike hyphae at margino
P. odoratum Christensen et Backus
bb. Colonies not developing stolon-like hyphae at margin
P. abeanum Smith
3'. Reverse usually in bright orange-red to orange shades.
aa. Colonies upon CA growing rapidly and spread ing widely.
P. luteo-aurantium Smith
bb. Colonies upon CA growing rather restrictedly
P. multicolor G-M et P. (see P. implicatum series)
4'. Colony reverse in bright yellow shades .
aa. Colonies upon CA in pale cream shades, reverse in b right
sulphu r-yellow colour.
P. cremeo-griseum Chalabuda
Page
. . . . 50
. . . . 51
. . . . 54
. . .. 67
. . . . 65
. . .. 63
. . . . 84
. . . . 59
Briely characte rized , sorne rnernbers of this series are a rnong the rnost
widd y di stributed a nd rnost abundant of all Penicillia . Sorne of th ern ,
cspcc ially Penicillium frequentans W estling and P. sp inulosum Thorn , a re co rnrno n
in soil a nd upon organic rnat erials under going slow decornpositi on. R aper a nd
T horn ( 1949) included in this series three well-defined sp ecies aggregates
ccntcred arou n d P. frequentans W estling, P. purpurescens (Sopp) Raper el Thorn,
nnd P. spinulosum Thorn , characte rized by con side rable varia tion within each
spccies aggregate, with , not infrequently , strain s bridgin g th e lin es of
sc pa ration between th ern . Since 1949 , how ever, six Penicillium species have
hccn described as new to science which see rn to fit quite well within th e series;
n.u nely P. cremeo-gnseum C hala b uda , P. griseo-azu reum M oreau el M oreau ex
R um rez , P. luteo-aurantium Srnith , P. odoratum Christensen el Backus, and P.
tarraconense Rarnrez el M artnez.
172-177. 1949.
Synonymies
Citromyces albicans Sopp - Monographie der Pilzgruppe Penicillium, pp.
128-129. 1912.
Penicillium aurantio-brunneum Dierckx - Ann. Soco Sci. Bruxelles. 25: 86. 1901.
Penicillium candido-fulvum Dierckx - Biourge's Monographie, La Cellule. 33:
275-277. 1923.
Penicillium columnare Thom - The Penicillia, pp. 214-215 . 1930.
Penicillium f1avi-dorsum Biourge - Monographie, La Cellule. 33: 290-291. 1923.
Penicillium fluitans Tiegs - Deut. Bot. Gess. Berlin. 37: 499-501. 1919.
10~m
~(I
-----==---< ,
'OoPm
(9 ~M~ (~fi
'i~) t;!j
~\~ .- Jt~~;~
'"'ihO;- ,,:"~.
f j
R.
499-501. 1927.
Penicillium pfefferianum (Wehmer) Pollaci - Atti 1st. Bot. Univ. Pavia. Ser. 11. 16:
IllItar obovo or below the surtace from whlch abundant crowded conidiophores
11110, volvoty, but commonly showing limited trailing hyphae, heavily sporing,
IlflU[llIy colandine to artemisia green (Sguy, PI. XXVI, 388, 389), becoming storm
Iff1 Y In oge (Sguy, PI. XXVII, 402), in some strains darker, approximating
morlcun to dark American green (Sguy, PI. XXIX, 427); exudate Iimited, c1ear to
lIuhl nrn ber: odour faint, 'mouldy'; reverse mostly in shades of yellow-orange near
1I1 ~"ol to mars brown (Sguy, PI. XIV, 1961, but in occasional strains appearing Iight
purpluh brown' (colour plate 9, a).
IllItes ollow :
I'l tipes 01' thc con idio pho res short , up to 200 tut: in length by 3.0 - 3.5 .tm in
d lrllllc!el' (lig . 9 , A) , with walls sm ooth or finel y roughen ed , enla rged at th e
'PI' X in a vesicle up to 5.0 .tm in diamet er; con id ial structures abundantly
1'1'1It1l1ced in new isolates, forming a cro wde d, fairl y contin uo u s layer over th e
whulc colony su rface ; penicilli almos t entire ly simple but showing an
11t' I'ltsillnal bran ch ; phialid es in crowde d clu sters of 10-12 or m or e in th e
\'l1 l't ir-i l, mostl y 8 - 12 .tm by 3.0 -3.5 .tm; usually producin g chains of con id ia
I1 I'lIi rly well-define d colu m ns up to 150 .tm or more in length (lig. 9, C) ,
flnll' limcs splitting in age , in othe r strains showing conid ial chains only loosely
pnrullcl; conid ia globose to su bglobo se, with wal1s com paratively thin , smooth
111' Iiu cly rou gh en ed (fig. 9 , B) and (SEM plate 2, centre : left) mostly 3.0-3 .5
1111 1 in di amet er.
( :lIll1 l1 ies upon C YA (colou r plate 9, B and b) spreading rapidly a nd
bl'l,atlly, a ttaini ng a di amet er of 50- 60 mm in 14 days at room temperature
~ () '2 ~ OC ), azonate, velve ty, radiat ely furrowed, close tex tu re d as aboye;
hl' ltvily spo r ing in va rious shade s of gree n, sometimes ob scured by ove rruw ihs ofw hite ae rial h yphae inf1uen cing th e colony appearance; thin m argin
with subme rge d hyphae exte ndi ng about 5 mm beyond th e aerial growth;
nxud.uc abu ndantly produced in sorne strains, limited or lackin g in othe rs, in
1111 1)(:1' shade s; odo ur pronounced, ' mouldy' ; dark amber-coloured pigm ent
d llTl lsing int o th e su rro u nd ing a gar in sorne strains, ab sent in othe rs; colon y
I'I: VCI'Se (colou r plat e 9, b) in pale orange , reddish brown to light cin namo n
luul cs, Penicilli as described aboye; conid ial cha ins in well-d efined colu m ns ,
uxuull y lon ger th an upon CA, up to 300 .tm long.
(:lI lonies upon MEA (colour plate 9, C and e) spreading rapidly a nd
1ll'lIadl y, attain ing a di amet er of 55-65 mm in 14 days at room temperature
tl () _ '25 OC ) , velvety, plane, loos e textured, zonate a t m argin, with submerged
iuycclia exte nd in g largely (about 5 mm) beyond the ae rial gr owth , loose
re..cturcd ; heavily sporing throughout in dark , yellow -gr een shades (Sgu y ,
1'1. XXI , 301), with centre showing artemisia gr een colou rs (Sguy, PI. XXI ,
'.110) and a n ove rg rowth of f1occose aerial hyphae ; exudate lacking in sorne
tra ius, prese nt in othe rs ; odou r pronounced , ' mo uldy' ; colony reverse (colou r
plruc 9, c) in light reddish cinna mo n shad es, with centre dark brown . P eni cilli
( ) I I I'
10. Penicillium purpurescens" (Sop p) R aper el T hom : fig. 10, colour plat e
10, and SEM pIate 2 (centre: right).
:@~:c~
0
e-;
; B00
@
O
lulllwJ.J
tf'>.
10 )Jm
'(j)
'~~'.
'_. -l-c{,l1
L--...J
100 )Jm
*The original spell ing of P. purpurrescens has been treated as a typographical error and correc ted
here to purpurescens.
'enlcllllum p rpurescens (Sopp) Raper e t Thom - 'A Manual of the Penlclllla'. pp.
177-180. 1949.
;ynonymies
Citromyces virido-albus Sopp - Monographie der Pilzgruppe Penicillium. pp. 131132. 1912.
11. Penicillium spinulosum Thom : fig . 11, coIou r pIate 11, a nd SEM
pIate 11 , and SEM pIate 2 (bottom: Ieft).
.f
.~ ~
:;
j
,o
,:.:
':
..:
'
: :~
'o
"o,
r.0 ,
0 0
r0
'o
'.
l"':
o,
0
o'
!;~~;o't~
tJ!" @
IV
(J'
[\1
e
L--..J
100 pm
(j(,
Pimlcllllum spinulosum Thom - Bul!. Bur. An lm . lnd ., U.S . Dep. Agr e. 118: 76.
1D10. See also Thom, 'The Penicillia', pp. 183-184. 1930; also Rapar and Thom,
fA Manual of the Penicillia', pp. 180-185. 1949.
yn on y m ies
nici/lium [Citromycesl brunneo-viride v. Szilviny i - Zentbl. Bakt. Parasitenk.
Inr. Hyg . 11 Abt. 103: 145. 1941.
nicillium (Citromyces) bruntzii Sartory - C. R. Soco Biol. Paris. 76 : 605-606.
1914.
nici/lium (Citromyces) citricus Maz et Perrier - Ann. Inst. Pasteur Paris, 18:
58-559. 1904.
nicillium f1avo-cinnereum Biourge - Monographie, La Celiule. 33: 293-295.
1923.
'onici/lium (Citromvcesl lacticus Maz et Perrier - Ann. Inst. Pasteur Paris, 18:
58-559. 1904.
Ponici/lium mediocre Stapp et Bortels - Z. Bakt. Parastenk. Inf . Hyg. 11 Abt. 93:
O. 1935.
Penici/lium mucosum Stapp et Bortels - Z. Bakt. Parasitenk. Inf. Hyg . 11 Abt. 93:
1. 1935.
Penicillium pfefferianum Wehmer - Beitr. Kenntn. einheim. Pilze, 1: 22-24. 1893.
Penici/lium p fefferianum (Wehmer) Westling - Ark . Bot. 11: 132-133. 1911; also
Biourge's Monographie, La Cellule. 33: 303-305 . 1923.
Penici/lium (Citromycesl oxalicum Maz et Perrier - Ann. Inst. Pasteur Paris, 18:
558-559. 1904.
Penicillium roseo-maculatum Biourge - Monographie, La Celiule. 33 : 301-303.
1923.
Penici/lium viridi-dorsum Biourge - Monographie, La Celiule. 33: 306-307. 1923.
Penici/lium tannophagum Stapp et Bortels - Z. Bakt . Parasitenk. Inf. Hyg. Abt.
11. 93: 52. 1935.
Penici/lium tannophilum Stapp et Bortels - Z. Bakt . Parasitenk. Inf. Hyg. Abt. 11.
558-559. 1904.
submcrgcd hyph ae cxtcnd ing 5 0 1' more mm be yond the acr ial growrh; odo ur
very Iain t 0 1' nbse nt; exudare lackin g 01' limit cd in amou nl, crystal clcar ; colony
reverse (colour plato 11, a ) almost colou rlcss lo palc gra y, occas ionally show in g
light p inkish to crearn shades. Pcnicill i usu ally strictly m on overticillat c but
with a n occa sion al branch ; stipes 01' th e con idio pho res a rising m ainly from th e
su bs tratu m 0 1' from ae rial hyphae, va ry ing from ver y long wh en arising
directl y from th e su bstratu m, 80- 100 .tm up to 300 .tm in len gth , to qu ite
short, 25 -50 .tm wh en arising as b ranch es fro m ae rial vege ta tive hyphae ,
mostly 2. 5-3 .0 .tm in di amet er, with walls smooth in sorne strains to definite1y
rou gh en ed in others (fig. 11, A), with ap ices enla rged in a ves icle up to 4.0 .tm
01' m ore in diameter ; phialides few in number, abo ut 6 - 10 in th e ve rticil,
m ostly 6-9 .tm by 2.5-3 .5 .tm; conid ia mostly globose to su bg lobose , usu all y
3.0 -3 .5 .tm in di ameter , sorne larger , up to 5.0 usu, cons picuo us ly rou gh en ed ,
spin ulose, sho wing windin g bars in so rne strains (fig . 11, B, and SE M plat e 2
(bo tto m: left ) ; conid ial chains forming loose colu m ns 100-1 50 .tm lon g (fig.
11, C) .
Colonies upon C YA sp read ing broadl y , a tta ining a di amet er 01' 60 -65 mm
in 14 days at room temperature (20- 25 OC ) (colour plate 11, B and b) , deepl y
ra d iately furrowed , with m yce1ial felt some what heavier an d more lanate than
upon CA , with centre depressed and irregul arl y fold ed; thin m argin with a
fringe 01' submerged hyphae extend ing abou t 3 mm beyond the aeri al gr owth ;
sporing well in m arginal areas in dark gr een , almost black shades, with
nonsporing areas remaining white to light tan ; exudate and odo ur lacking ; no
pi gm ent di ffusin g into the surrou nd ing agar. Peni cilli as described aboye .
Colonies on M EA (colour pIate 11, C and c) gro wing rapidly attaini ng a
diameter 01' 50- 60 mm in 14 days at room temper ature (20 -25 OC), velve ty,
plan e , thin m argin with a frin ge 01' su b me rge d hyph ae extend ing 4- 5 mm
beyond th e ae rial gro wth, usu ally showing a loose-t extured , white lanat e felt
abo u t 2-3 mm deep ; heavily spo ring throu gh out in shades 01' very dark gree n
(Sguy, PI. XXIX , 431); odo ur and exu da te lacki ng ; no colou re d pigment
diffusin g in to th e su rro u ndi ng agar. Peni cilli as described aboye but with th e
stipes 01' th e conid iopho res cons picuo us ly ech in ulate and with conid ial chains
showing a greate r tenden cy to form 100se1y paralle1 colu m ns.
Sp ecies descriptio n concern ing cult u ra l characte ris tics are based upon the
typ e strain N RRL 1750 = C BS 374.48 = C M I 24,3 16 = ATCC 10,498 = QM
7,654 = IJFM 7096 , and upon observations m ade on a large number 01' stra ins
havin g essentially the same cu ltural and m or phological characte ristics. Strain
CB S 269. 29 = IJFM 5035, isolat ed by Biou rge , no longer represents th e species
ade q uate1y with respect to cult ural ap pearance. It is now esse ntially lanate and
almost sterile but still produces penicilli and con id ia characteristic 01' P.
spi nulosum.
Penicilliu m spinulosum T ho m is world wide in di stribution , espec ially com m on
I:.! . Perucilliurnn cremeo-griseum ChaIabuda: fig . 12, colou r pIate 12, and
S EM pIat e 2 (b attam : ri ght) .
"
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B
v.:
lwJJ.w.lJ
10 pm
~
.
I'."""""",,-'t,,
-"
~
"
', '
.-.
So~\..
';ii
R.,
;Ji
10 0
jrm
Colon ies upon CA (colour plate 12, A and a) spre ad ing fairly rapidly ,
a llain in g a di amet er of 50 mm in 14 da ys at room temper ature (20- 25 OC ),
co ns istin g of a very tou gh , cla se textured basal felt, vel vet y in a p peara nce ,
rad iatcly su1cate, dcpresscd in centre ; eve nly and thin murgin with a frin gc 01'
sub me rge d hyphae extcnding abo ut 1- 2 m m beyond the acrial gro wth, a t firs t
white , th en bccom in g sulp hur ycllow to pale crca m in age; exudate ora nge
colou red ; odo ur faint , 'mouldy'; colony surface cove red by a network of
tra ilin g vege ta tive hyphae and rop es of hyphae; pale a m ber pigm en t diffusing
into the su rrounding agar; eolony reverse (eolour piate 12, a) in yellow, eream,
to reddish shades; pe nie illi strietly simple, seldo m with an oeeas ional brane h
(fig . 12, A) ; the stipes of th e eonidio pho res arisi ng from su bmerged , ereeping
01' funieulose hyphae, u su all y short, 20 -40 tut: by 1.0- 2.5 tui, sep tate, with
wa lls smooth 01' del ieately rou ghen ed at upper parts, wit h apiees en la rged in a
vesicle 2.5 -5.0 tm in diameter; phialides fewin nu mber , 2-6 per verticil,
mos tly 6.0- 11.0 tm by 3.0 tm; eonid ia globose to subglobose, eomparatively
small, 2.0 -3 .0 tm in dia me ter, delieately rou ghen ed (fig. 12, B, and SEM
plate 2 (bo ttom : rightj) ; eonidial ehains forming loose columns up to 300 tm
lon g (fig. 12, C) .
Colon ies upon CYA (eo lour plate 12, B an d b) almost d up lieating in cultural
eharaeterist ies those grown on CA , attaini ng a diameter of 50-55 m m in 14
days at room temperature (20-25 OC), deeply velvety to somewhat lanate ,
white; growing margin thin, with a fringe of submerged hyphae ext end ing
abo u t 1- 2 m m beyond the ae ria l growth ; abundantly sporing in whi te to pale
gray shades; odour faint, indefi nite; exudate in yellow droplets; eolony reverse
(eolour plate 12, b) in pale yellowis h eream shades; light amber-colou red
pigm ent diffus ing into the surrounding agar; pen ieilli as deseribed aboye .
Colon ies on malt-extraet agar (colou r plate 12, C and e) spreadi ng broadly,
attaining a dia meter 01' 50- 60 mm in 14 days at roo m te mperature
(20-25 OC), plane , deepl y velve ty to su b floccose, ow ing to a thi n overgrowth
01' wh ite aerial hyph ae lend in g th e eolony a lan at e appearanee; thi n margin
surrounded by a fringe of submerged hyphae exte nd ing 2-3 mm beyond the
aerial growth, eonsistently heavier spo ri ng throu gh ou t in mou se-gr ay shades
(Sguy, PI. XL, 598) ; odo ur pleasant, aromatie; exudate laekin g ; yellowis h
green-eolou red pigm ent diffus ing into th e surrou nding agar; eolony reverse
(eolour pla te 12, e) in yellowis h green shades . Pen ieilli as des eribed above.
Spee ies dese ription is eente red upon the type strain CBS 223 .66 = ATCC
18,320 = BKM F - 1,034 = IJFM 5011 , isolated from Russia n fores t soil in 1949
by T. V . C ha labuda, in th e env iro ns 01' Ki ev.
hl
-fJL! =!
r l1
())J
:----:: ~
\jxv/
0 O 10).lm \. \'./.,
00 8 @ .. 0
O
@ (,.::.
...'Y
00
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00 ' 0
~
0 fD OO~8
Oo OOoOO O
00 0
v, "
~j '~;f\)
~' '' ~\.
~
, <::.~
~ \""'-,lb
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"
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100 jrm
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'
~~.
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fR.,
Penicillium griseo -azu reum was isolated from sand dunes in France by Drs F .
and V. Moreau (1941) , who prop osed th e name P. griseo-azureum and des cribed
I he spec ies without providing a L atin di agnosis . To validat e th e species a Latin
diagn osis of it is give n below ; the names of th e origin al au thors are maintained
under Recommendation 46 C of the International Code of Botanical Nomendature (Stafleu (Ed.), 1972).
Latin diagnosis of Penicillium griseo-azureum Moreau el Moreau ex Ramrez:
Coloniae in agaro Czapekii satis celeriter crescentes, post duas hebdomadas ad
20-25C, 35 mm in diametro assecutae sunt, velutinae, denso texto basali coacto
mycelio, leviter sporulantes, primum albae, deinde griseolo-coeruleae, inaequaliter
prominentes, plicatae, radiatae, cum aequabili margine, parum elevatae ad
centrum; exsudatio adest, exiguus; odor abest; reverso cremeo-albido; neque
ascomata neque sclerotia visa sunt. Stipitibus erectis, brevis, glabris, hyalinis,
plurimum e mycelio basali vel e hyphis reptabundis, vel etiam ex substrato
exoriuntur, plerumque usque 100 flm longitudine per 2.0-3.0 flm in diametro, cum
apicibus tenus 5 flm inflatibus; penicilli pleraque monoverticillati, rami rari; phialides
pauper numero, 2-6 per verticillum, plerumque 5-11 flm longitudine per 2.5-4.0
flm in diametro. Conidia globosa vel ovoidea, plerumque 2.0-3.0 flm in dimetro, in
lat e 1:" b) in pal e ora ngl: lo crcarn shndcs: pcni cill i as describod abov... .
Colonies upon M EA (eolour plate 13 , a nd e) spread ing b road ly, attaini ng
d iameter of 40 - 50 mm in 14 da ys a l room tempcrature (20 -25 OC ) , planc ,
leeply velvet y , heav ily spori ng throu gh ou t in m ou sc-gray shade s (Sgu y , PI.
{XI , 307), eve nly m argin in lighter gray tin ts , with a frin ge of su bmerge d
iyphae exte nd ing largely beyond th e ae rial gr owth (about 4 mm); odo ur a nd
.xu d a te absent ; pale am be r eoloured pigm ent di ffusin g int o th e su rrou nd ing
19ar; rever se (eolour plat e 13 , e) in pale or ange shades . Penieilli as deseribed
ibove but u su all y a rising direetly from th e su bs tra tu m, and with eonid ial
ch ains forming loose to tangled eolu m ns .
Sp eeies deseription is eente re d upon th e typ e stra in CBS 162.42 = ATCC
18 ,228 = Q M 8, 150 = IJ FM 5010 , isolat ed from sa nd dunes by Drs F . and V .
M or eau, in Franee, in 1941.
00 0
00 0
Gas o ooo
O
0
O O
; 0 o
lJ.J.J.il.uu.J
10
jrrn
Aj-~
L---J
)Jm
100
iR.
Penici//ium luteo -sursntlum Smlth - Trans, Brlt, Mycol. Soco 46: 331. 1963. See
also M.M. Kulik, 'A Compilation of Deseriptions of New Penici//ium Species' .
Agrie . Handbook No . 351. Agrie . Res. Ser. U.S . Dep. Agrie. , p. 50. 1968.
C olon ics upon C YA (colour plat c 14, B and b) uttai ning a di a mcter 01'
Colonies on C A (colour plat e 15, A and a) growing fairly rapidly, att a ining a
diamet er 01' 55 mm in 14 days at room temperature , consis ting 01' a loosetextured basal felt , covered by an overgrowth 01' ae rial vegeta tive hyphae
lending a su blana te appearance to the otherwise velvety colony surface , almost
plane, heavily sporing in sorn e area s in gray-gree n shades (Sg uy , PI. XXIX,
432) ; evenly m argin with sub me rge d m ycelia exte nding 3-4 mm beyond th e
{ :,~ '.;
ae rial growth; od our and exudate lacking ; colony rev ers e (colour plat e 15, a )
colou rless; penicilli strictly sim ple (fig . 15, A), with stipes usu ally arising fro m
trailing aerial hyphae, so rne from su bme rge d hyphae , variable in len gth , up to
170-440 p.m by 2 .0-3 .8 tui, with apices enla rge d in a vesicle up to
3 .5- 5.0 p.m in di ameter, with walls smooth or delicately rou gh en ed ; phialides
numbering 5 to 8 in th e verticil, mostly 8 .0 - 15. 0 p.u: by 2.5 -4 .0 p.m , wi th a
short, de finite ly narrowed neck ; con id ia cons picuous ly rou gh en ed (fig . 15, B),
show ing a cere briform pattern whe n obse rve d under th e sca nning elec tron
mi cros cop e (S EM plate 3 (centre; left )) , mostly 4.0 - 5.0 p.m in di amet er,
globo se ; conid ial chains forming long, loose colu m ns (fig . 15, C).
Colon ies upon CYA (co lour plat e 15 , B and b) spread ing broadl y , attaining
a di ameter of65 mm in 14 days at room temperature (20-25 OC ), cons isting of
a similarly textured felt as described aboye but with sh allow radiate furrows ,
heavier spo ring in dark gray-gree n shades (Sg uy, PI. XXX , 446) in m argin al
areas; con id ial struc tu res obsc u red in su bmarg inal and central a reas by a n
ove rg ro wth of white aerial hyphae lending a lan at e appearance to th e colony ;
odo ur and exuda re lackin g ; co lony revcrsc (colou r plute 1;, b) in vc ry palc
ycllowi sh tint s , Peni cilli as desc ribcd a boye.
Colon ies O I! M EA (co lour pla tc 15, C and e) gro wing stilJ more rapidl y th an
up on the aboye substrata, a ttaining l diarnete r 01' 70 mm in 14 days at roo m
tempcrature (20-25 OC), vclvet y in appearance but cove red by an abu nda nt
ove rgrowth of ae rial hyph ae lending a lan at e appeara nce to the colony surface;
heavily sporing in dark gray-gr een shad es (Sguy, PI. XXX , 446), bu t con idial
structu res are partly obsc ured by the mention ed ove rg rowth; exudate a nd
od our lacking; penicilli as described abo ve but with coni dial chains forming
well-defin ed com pac t colu m ns.
Penicill ium abeanum Smith was isolat ed fro mJapa nese soil by S . Ab e in 1952,
and described by K. Sa kaguchi a nd S . Ab e (Abe 1956) as P. trzebinskii Zaleski
va l'. magnum, a nd red escribed by G . Smith ( 1963) as P. abeanum.
Species descript ion is centere d up on th e type strai n CB S 348.59 = C M I
68 ,222 = I Fa 6,239 = IJ FM 5048, being in sa tisfacto ry ag ree me nt with the
original spec ies di agn osis.
16 . Penicillium odoratum C h riste nsen et Ba ckus : fig . 16, co Io ur pI ate 16 ,
a n d SEM pI ate 3, (ce n t re: ri ght) .
'~$~f~ ~'
'~I r~G
~
jrm
10
()
R.
100 ).1m
a nd has a vun illa -likc odo ur instcad 01' the apple-likc aro ma so cha ractc ristic 01'
P. odorat um, has sho rte r stipcs (60- 2110 .u n lon g) with som cwh at sm aller
ves icu lar api ces, a nd does not fo r m stolon-like hyph ae up on C YA a nd M EA.
Spe cies dcscription is ce nte rcd up on the ty pe strain C BS 294.62 = ATCC
14,769 = F 7.741 = IJ FM 5005, isolat ed from the pcat y soil 01' a spruce -Iarc h
com m u nity in Taylor Cou nty, Wi scon sin, U SA, by M. C hriste nse n and M .P.
Backu s, in 1960 .
17. Penicillium tarraconense Ramrez el Martnez : fig. 17, caIaur pIate 17,
and SEM plate 3, (ba tta m : Ieft).
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vcsi clc IIp to 5 .0 mI in di um ct c r: phi ali d es bo ttlc-sh np ed with sho rt -poin tcd
neck , co m m o n ly 10.0 -1 5.0 mI in lcn gth b y :U l- 1. 0 LIlI in diumct cr,
di vcr gent , 6- 10 in thc vet icil (Iig. 17, A) ; co nid ia smoo th-wallcd 0 1' ncarl y so
(lig. 17, B, and SEM plate 3, (bottom : left , clliprical , usu all y 3. 0 - 1 .0 un by
2.5- 3.0 JL m in diamete r; co nid ial chains tangled and penicilli str ictly simple
(lig . 17, D ) .
Colonies upon C YA (colour plat e 17, B a nd b) gro wing luxuriantly,
attai ning a di amet er of 60 m m in 14 d ays at roo m temper ature (20-25 OC),
velvet y , almost plane , compact, close- textured , sligh tly rai sed in central a re a;
heavily spo ring th ro ug ho ut in yellowish to gray-gree n shades (Sguy, PI.
X XIX , 424) in margi nal a rcas, and in pale oc h re shades in cen tre; the colony
surface is covered by a n ove rg rowth of loose floccose hyphae , especially in
cen tra l areas; ex udate lacki ng ; odo u r faint , indelinite ; colon y reverse (co lour
plat e 17 , b) b rown in ce nt re and in oc hraceous shades in m argin al a nd
submarg inal a reas; a m be r-colou red pigm ent d iffus ing into th e surro u nding
agar. No a scomata 01' scle ro tia have been obse rved, but a few structures
sugges ting ascomata initials have been observe d (lig. 17, C). Penicilli strictly
sim ple, with lon g stipes up to 200 JLm by 2.5 - 4. 0 JLm co m monly borne fro m
submerged h yph ae , ei the r smooth 0 1' ro ugh-walled, as described aboye .
Colonies upon MEA (co lour piate 17, C and c) gro wing rathe r m od erat ely ,
atta ining a dia m et er of 40 mm in 14 da ys at roo m temper ature (20- 25 OC),
pla ne , ve lvety, loose textured and sligh tly zo nate; heavil y sporing throu ghout
in yellow- gr een sha des (S guy, PI. X X IX, 424); thin , eve n marg in su rro u nde d
by a fringe ofs ubmerged hyph ae exte nd ing 3-4 mm beyond th e aerial growth;
exudate not ob served ; odour fragrant ; revers e colou rle ss with a gree n cas t
show ing throu gh ; no co lou re d pi gm ent di ffusin g into th e su rrou nd ing agar
(co lour pla te 17, c) . Penicilli as aboye.
Penicillium terraconense R amrez el M artnez a ppears m ost closely related with
species in cluded in th e P. frequentans se ries on accou n t of its str ictIy sim ple
pen icilli , co m paratively la rge , with lon g and erect stipes a ris ing di rectl y fro m
th e su bstrat u m, of th e velve ty a ppeara nce of th e colon ies, and of th e lu xuriant
growth on most m edi a use d, but det ail ed in vesti gati on s fail ed to m at ch it with
any of th e speeies in th e series.
Species di a gn osis is based on th e type stra in IJFM 5151 = ATCC 42,235,
isolat ed from Spanish must , M ad rid , 197 8 . It is at presen t known only fro m
th e typ e strain.
Penicillium lividum se r ie s
UT ST A NDIN G FEATU RES
r ile stipcs 0 1' the conidiophores are ercc t 01 ' asccndi ng , lon g , usua lly
unb run chcd , w ith wa lls usually delica tcly rou g hcn cd a nd with a p ices
so rncw hat enlarged in a vcsicle .
Pcni cilli strictly simple with phialides usu ally cro wde d in the vert icil, parallel
0 1' div crgen t .
C o nid ia elliptical to su bglobo se , mos tly 3.0- 4. 0 Jlm in lon g axis, with wa lls
echinu la tc, often cons picuously .
Key to species
1. Colonies deeply lanose, stipes very long, up to 400 p.m 01' more; reverse
in orange to ochraceous shades.
a. Penicilli with phialides comparatively large, up to 14 p.m in length
1' . Conidia compa ratively large, up to 6 p'm in long axis, conspicuously roughened.
P. lividum Westl ing
2'. Conidia comparatively small , up to 3.5 p'm in long axis, with walls
smooth 01' nearly so.
P. valentinum Ramrez et Martnez
b. Penicilli with phial ides comparatively small, up to 10 p'm in length.
Conid ia elliptical with pointed ends, with walls delicately roughened.
P. aurantio-violaceum Biourge
2. Colonies not deeply lanose, deeply velvety, stipes comparatively short.
a. Penici lli with phialides comparatively large, up to 10 p'm in length;
odour suggesting aroma of vanilla ; reverse in pale ochraceous shades.
P. trzebinskianum Abe ex Ramrez
b. Penicilli with phialides comparatively small , up to 8 p'm in length;
odour lacking 01' indefinite; reverse in deep violet to violet-black
shades.
P. trzebinskii Zaleski
Page
. . . . 72
. . .. 80
. . . . 74
. . . . 78
. . . . 76
The members 01' th is series represent a normal com po ne nt 01' th e fungu s flora
01' soils, but occurring less frequently tha n othe r sim ple forms. T he seri es
actually com prises five well-d efin ed spec ies:
Penicillium lividum W estling, easily recog nized by its deep velv ety , almost
lanate colon ies, its typicall y blue-green colou r, and its conspicuo u sly rough walled , elliptica l conidia . Penicillium aurantio-violaceum Biourge , although
presenting m u ch the same cultu ral cha rac te ristics, differs, however, markedl y
in producin g strongly elliptica l con id ia, usuall y wit h poi nted ends . Penicillium
trzebinskii Zaleski, characterized especially by the colony revers e in deep violet
to violet-b la ck shades. Penicillium trzebinskianum Ab e differs from P. trzebinskii by
the produ ction 01' a strong vanilla-like a rom a, its com paratively large penicilli,
its Iaster grow ing colon ies up on C YA, an d thc prod uction 01' l rcddi sh brown
pigm cnt in thc colony rcver sc, which diffu ses into thc su rro u nd ing agar, upon
MEA . And finall y, Penicillium oalentinurn Rarnfrez et M artfnez , more closely
related to P . lividum West1ing, characterized by its dceply velvety , almost
lanat e colonies, its com pa ra tively large peni cilli , and the ora nge sha des
developed in colony reverse. The seri es appears to be related to the P.
frequ entans series throu gh P . trzebinskii, P. sp inulosum and P. odoratum .
18. Penicillium lividum W estling: fig. 18, colour plate 18, and SEM plate
3, (bottom: right) .
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Penicillium lividum Westling - Ark. Bo1. 11: 58, 134-137. 1911. See also Dale, Ann.
Mycol. 12: 52. 1914; also Thom, 'The Penicillia' , pp. 205-206. 1930; and Raper
and Thorn, 'A Manual of the Penicillia', pp. 190-192. 1949.
iou gh basal fclt, loosely lextured al the colon y sur lace whi ch is decply velvcty
l o almos t lanate , a t Iirst white, then assurning deep blu e lo blu e-green shade s
in heavily sporing zon cs (Sguy, PI. XXXI, 461 -463), slightly zonate, with
undulating margin white , surrounded by a narrow fringe 01' submerged hyphae
cxtcnd ing about 1 mm beyond the aerial growth; exudate lacking; odour
nhsc nt or indefinitc ; colony revers e (colour pIate 18, a) in orange to peach
shndes; pale arnber-coloured pigment diffusing into th e surrounding agar;
pcni cilli mostly strictly sim ple, with stipes 01' th e conidiophores mo stly arising
Irorn the substratum , usually unbranched, comparatively very long, up to
(iOO .tm or more in len gth by 2.5-4.0 .tm in diameter, septate , with walls
xmooth or nearly so , with apices enla rged in a vesicle up to 5.0 -6 .0 .tm in
rlin m ete r (fig . 18, A) , or arising as short branch es from a network 01' aerial
hyphae; phialides in parallel clusters or somewhat divergent, mostl y 5-10 in
(he vcrticil , comparatively large , 11.0-16 .0 tut: by 2.5-4 .0 .tm, terminating
in a fairly conspicuous tapered neck; conidia at first elliptical, then becoming
usually ovate to subglobose at maturity, mostly 4.0-5.0 tut , occasionally up to
(i.O .tm by 3.0-4 .0 tuu, with walls conspicuously roughened (fig . 18, B) ,
usually in spiral bands when observed under th e SEM (plate 3, bottom : right) ;
conidial ch ains short and tangled (fig . 18, C).
Co lonies upon CYA (colour plate 18, B and b) spreading more rapidly than
upon CA, attaining a diameter 01' 55 mm in 14 days at room temperature
(~ O-25 OC ), essentially duplicating in colour , cultural ch ar acteristics and
det ails 01' morphology those described up on CA, but deepl y radi ately furrowed
with centre depressed; exu date a nd od our lacking; colony rever se (colour plat e
In, b) in darker orange or cinnamon shade s; amber coloured pigment diffu sin g
into the surrounding a ga r. Penicilli as described aboye.
Colonies upon MEA (colour plate 18, C, and c) growing fairly rapidly ,
a ttainin g a diameter 01' 55-60 mm in 14 day s at room temperature, plane,
dcepl y velvety to Ian ate , he avil y sporing throughout in deep blue-green shades
(Sguy, PI. XXX , 446) ; od our sIightly ' mou ldy'; exudate Iacking; pale am be rcoloured pigm ent diffusing into th e surrounding a ga r; revers e (colour plate 18,
c) in orange shades ; penicilli as described aboye but with the stipes 01' the
con idiophore s arising directly from the su bs tratu m, and comparatively very
lon g, up to 1000 .tm or more , with walls frequently roughen ed in terminal
a reas; penicilli av erage a little larger than upon CA; con idia more cornmonly
rem aining ellipticaI at maturity, otherwise as described aboye.
The species is centered upon the type species ATCC 10,102 = CBS 347.48 = CMI
39, 736 = IFO 6 ,102 = NRRL 754 = IJFM 5,155 , isolated from soil in Scotland by
Miss E. Dale , in 1913, Westling' s type strain being lost before 1911. It constituted the principal basis for Thom' s redescription 01' Westling species (1930).
An other cultu re received from the Centraalbureau voor Schimmelcultures ,
Baarn , The Netherl ands, labeled Penicillium lividum W estling CBS 217 .30 ,
produ cti on of (hin , closc-tcxtu red , more rcs tri ct cd colonics up on C A, a ttain ing
a di amet er of 25-30 mm in 14 day s at roo m tempcr ature ; peni cil1i with sho rt
stipes, ra rely more th an 50-100 tm lon g. Upon M EA th e colo nies a re
spread ing b ro adl y , a tta in ing a diamet er 01' 60 mm in 14 days a t roo m
temperature , plan e , heavil y sporing th rough ou t , with th e margin very
irregul arly di ssected from th e uneven gro w th 01' th e veget ative mycelium ,
whi ch is largely su b me rged . In colony charac te ristics, thi s peculia r strain fits P.
lividum only in th e colour 01' conid ial areas (Sguy, PI. XXXI , 461- 463),
deepl y blue-gr een . The penicil!i , however , agree q uite rea son abl y with the
a bo ve description ; thus this strain ca n no t be rem oved fro m th e species because
01' the co ni dial structu res produce d . A strain dupl icating in al! eharacte ristics
th e C ES 2 17.30 strain 01' P. lividum, strain IJ FM 5157, has been isola ted by th e
a u tho r from th e a ir in Mad rid, in 1978 .
Penicillium lividum W estl in g, altho ug h less ab u ndant th an many species, is
found amo ng isolates from soil, from sto re d cereal p roducts, and from org a nic
materials su bject to a ir 01' soil conta m ination, and is ap pare n tly cos mopo litan
in di stribution.
19. Penicill ium aurantio-v iolaceum Biaurge: fig . 19 , colou r pi ate 19 , and
SEM pla te 4 (top: left) .
Penicil/ium aurantio-vio/aceum Biourge - Monographie, La Cellule. 33: 282-284.
1923. See also Thom, 'The Penicillia' , p. 208. 1930; also Raper and Thorn, 'A
Manual ot the Penicillia', pp. 192-194. 1949.
Synanym
Penicil/ium roseo-viridum Stapp et Bortels - Z. Bakt. Parasitenk. Int. Hyg. Abt. 11.
93: 51. 1935.
Colonies upon CA (co lour plate 19 , A and a) sp read ing b road ly, attain ing a
d iameter 01' 55 -60 mm in 14 d ays at room temperature (20-25 OC), plane 01'
with shal!ow furrows , azo nate to sligh tly zona te , com para tivcly deep up to
1.5 mm in cent ra l arcas , co ns isting 01' a tough m ycelial felt at th e su bs tratu m
level , with su rface loose-t extured , deepl y vclv et y to lanate , formed by lon g ,
erect stipes; evenly m argin white , 10 -1 5 mm wide , rather thin, with
su b me rged hyph ae ex te nd ing a bo ut 2 mm beyond th e aeria l gro wt h; heavily
sporing th roughout in blue-green shades (Sg uy, PI. XXX , 443), re m aining
almo st unch anged in age; ex udate lacking ; odo u r faint , indistinct ; colony
reverse (co lo ur pla te 19 , a) in light cream to pal e salmon- pin k shades ; pen icil!i
strictly sim ple (fig. 19, A), with stipes 01' th e con id iophore s arising prim arily
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with walls smooth to closely and delicately echinulate , with apices somewhat
enlarge d in a vesicle up to 4.5-5 .0/lm in di amet er; phialides in crowded
clusters of 8-12 or mor e in th e verticil, slightly divergent a t th e tip s, about
8.0-10.0/lm by 2.0-2 .5/lm , terminating in a definitely narrowed neck ;
conidia strongly elliptical, almost fusiform , about 3 .0 -3 .5 /lm by 2.0-2. 5 /lm
in diameter, with ends usually pointed, with walls som etimes appearing
smooth, wh en obs erv ed under th e light mi cro scop e (fig . 19, B), but delicately
roughened wh en obse rved under th e SEM (plate 4 , top: left) ; conidial ch ains
forming eithe r parallel or div ergent colu m n s, up to 100 /lm or more in length
(fig. 19, C) .
Colonies upon CYA (colour plate 19, B and b ) growing as rapidly as upon
CA, attaining a di amet er of 55 mm in 14 days at room temper ature
(20- 25 OC ), duplicating in texture th e colonies grown upon C A , but deeply
radiately wrinkled, less heavily spo ring in artemisia green shades (Sgu y , PI.
XXVI, :i90); odour a nd exuda re lacklng: umbcr-co lourcd pigmcnt dillu sing
into the su r ro u nd ing ag ur; colo n y reve rso ( COIOlll' plat e t 9 , h) in Iight orange to
salmo n-pink shadc s ; pcni cilli as describcd aboye.
C olonies upon MEA (colour plat e 19, C a nd e) sprcad ing broadl y, attaining
a diameter 01' 60 -70 mm in 14 days al room tcmperature (20-2 5 OC ),
consisting 01' a loose-t extured felt , with wid e gro wing margin surrounded by a
fringe 01' submerged hyphae extend ing 5 mm 0 1' more beyond the ae rial
gro wth; heavily sporing throu ghout in yellow-green shade s (Sguy , PI. XV ,
222) , remaining almost unchanged with age ; odour and exudate la cking ;
penicilli as described aboye , but with the stipes 0 1' th e conidiophor es mo stly
arising from th e substratum , com mo nly more th an 500 .tm in len gth ; conid ial
chain s forming loose colum ns up to 200 .tm long ; colony rev erse (colour piate
19, e) in pale orange shades ; no colou re d pigment diffu sin g into th e
su rro und ing agar.
Sp ecies description is cente red up on th e typ e stra in CBS 267 .35 = CMI
39,740=ATCC 1,041=NRRL 760=IFO 6,089=QM 7,485=IJFM 5019, isolated
from a beech-forest soil in Berlin (G ermany) by C . Stapp an d H . Bortels in
1935 , and us ed a s th e typ e a l' th eir new spec ies, Penicillium roseo-oiridum ,
actually conside re d as syno ny m ous with P. aurant io-violaceum ea rlier described
by Biourge .
20 . Penicillium trzebinskii Zal eski: fig. 20 , colou r plate 20, a nd SEM plate
4, (top : ri ght) .
Penicil/ium trzebinskii Zaleski - Bull. Acad. Polonaise Sci. Math. Nat. Sr. B, pp.
489-499. 1927. See also Thom, 'The Penicillia', p. 189. 1930; also Raper and
Thom, 'A Manual of the Penicillia', pp. 194-196. 1949.
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cnlurgcd in a vcsicle IIp lo 1,,0 - 1'.;' J~1Il in dluntctcr, with walls conspicuously
cchi nu la te ; philialides crowd ed in the whorl , up lo 12 01 ' more in thc verticil ,
othe rw ise as dcscrihcd above ; con id ia l chains in loo se colu m us u p lo 150 tun 0 1'
more in lcn gth . Ex udate lack in g ; odo u r in dc fi n itc ; colony reverso (co lou r pIa te
20 , e) eolou rless 0 1' pale eream shades .
Speeies d eseription is ee ntered up on th e typ e strain CBS 382.48 = CMI
39,749=ATCC 10,50 7 = N R RL 731 =IF O 6, 110 =QM 7,678=IJFM 5008 , isolat ed
fro m fore st soil in Poland by K . Zaleski, in 1927 .
The eor ree t plaeement of thi s speeies still remains some what questi on a ble . It
was placed b y R ap er and Thom ( 1949) with the P. lividum se ries on aeeo u nt of
its lon g , rough stipes a nd eeh in ula te eo nid ia, th at are usu all y broadly ellip tieal.
The ove r-all pietu re of the spe cies, however, suggests a close relat ionsh ip to P.
spinulosum Thom.
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phialidcs crowded in com pac t cluste rs 01' 5-12 in the verticil , mostly
8.0 -12 .5 usu by 2.0 -3.5 ust, terminating in a short , tapered neck; conidia
ovate to subglobose, mostly 2.5 -4.0 J.m by 2.5 -3.0 J.m, with walls
conspicuously echinulate or verruculose (fig . 21, B, and SEM pIate 4, centre:
left) ; conidial chains forming well-defined compact or loose columns (fig . 21,
C) , up to 300 J.m in length .
Colonies upon CYA (colour plate 21, B and b) spreading broadly, attaining
a diameter al' 55 mm in 14 days at room temperature (20-25 OC), consisting al'
a compact basal feIt, deeply velvety, covered by a loase overgrowth al' floccose
aerial hyphae giving a sublanate appearance to the colony surface, strongly
radiately furrowed, with evenly margin 2 mm wide; heavily sporing
throughout in the same deep blue-green shades as upon CA ; odour suggesting
vanilla aroma; exudate lacking; colony reverse (colour plate 21, b) in
cinnamon shades ; penicilli as described aboye, but with conidial chains loosely
parallel to tangled.
Colonies upon MEA (colour plate 21, C and c) growing less rapidly than
upon CA and CYA, attaining a diameter al' 45 mm in 14 days at room
temperature, plane, deeply velvety to lanate in appearance, heavily sporing
throughout in dark, mouse-gray shades (Sguy, PI. XXXVII, 547); thin
margin surrounded by submerged hyphae extending about 5 mm beyond the
aerial growth ; exudate limited, colourless , enmeshed in the mycelial feIt ; odour
suggesting vanilla; colony reverse (colour plate 21, c) in cinnamon to reddish
brown shades; bright yellow coloured pigment diffusing into the surrounding
agar. Penicilli as described aboye.
Penicillium trzebinskianum seems to have a clase relationship to the P. lividum
series on account 01' the deep velvety to floccose character al' its colonies, its
long and rough-walled stipes, and its broadly elliptical and echinulate conidia.
The specific name given by Abe reminds its similarities with P. trzebinskii
Zaleski , especially the echinulate character al' conidia and the pigment
developed upon CA.
Spe cies description is centered upon the type strain CBS 431.65 = IFa
6,038 = IJFM 5041, isolated fromJapanese soil by S . Abe, in 1956.
22. Penicillium ualentinum Ramrez el Martnez: fig. 22, colour plate 22,
and SEM plate 4 (centre : right) .
Penicillium valentinum Ramrez et Martnez - Mycopathologia. 72: 181-191, 1980.
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Colo nics lIpon CY A (co lou r plat c 22, B a nd 1 sprcad ing b roadly an d
rapidly , a u a ining a di a mct er 01' G5 mm in 1'1 days a l roo m tcmpcraturc
(20-2 5 OC), vclvcty lo su b lanatc in appcaran cc , closc texturcd ; d ecpl y
radiately furro wed; m argin en tire with a narrow Iringe 01' su b me rgc d hyphae
ex te ndi ng abou t 1 mm beyond th e ae rial gro wth ; cen tra l are as depressed a nd
irregularl y fold ed ; heavily spo ring throu gh ou t in sim ila r blue-gr een shades as
up on CA; ex udate and odo ur laeking; reve rse in lighter ora nge sh ades (co lou r
plat e 22, b) ; no eo lou red pi gment diffu sin g into the surrou nd ing agar; pe nieilli
as deseribed above, with stipes 0 1' th e co nid iopho res smooth walled to defin itely
ro ughe ne d; eonidia l ehains tangled 0 1' forming we ll-de fine d eolu m ns.
Colon ies upon M EA (eo lour pl at e 22, C and e), a tta in ing a di amet er 01'
55 mm in 14 d ays at ro om temper atu re (20-25 OC), pl ane, velvety, with
centre sligh tly rai sed ; m argin eve nly wh ite , wit h su b me rge d hyphae extending
abo u t 5 mm beyond th e aerial gro wt h; heavil y spo r ing th rougho u t in bluegree n shades (Sguy, PI. XXX , 438, 449); eo lony surfaee eove red by an
ove rg row th 01' loosely interwoven floeeose hyph ae , influeneing th e eolony
a p pearanee; ex udate an d odo ur laeking ; eolony reverse (eolour pla te 22, e)
developing light ora nge shades ; a mber-eoloured pigment diffusing in to th e
surrou ndi ng agar; penieilli as deseribed a boye, with th e st ipes u suall y rou gh
wa lled, lon g , ereet, arising m ostly fro m th e su bs tratu m ; eo nid ial ehains
formi ng loose to eom paet eolu m ns .
Penicillium valentinum a ppears most close ly related to speeies included within
th e P. lividum series on aeeou n t 0 1' th e deepl y velo u tino us eharaete r 0 1' its
eolon ies, th e fast growth on a ll m edia tested , its long, rough- wall ed stipes, a nd
its ellip tiea l eoni d ia.
Speeies di agnosis is ba sed u pon th e typ e strain IJFM 50 71 = ATCC 42,22 7,
isola ted fro m th e air , M adrid , 1978. It is a t p resent kn ow n only from th e typ e
strain .
Colon ies growing restrie ted ly up on most substrat a , usu all y eo m paet,
velvety, heavily spo ring, m ostly in blue -green shades , with vegetative hyphae
sometimes ene rusted and p igmented; colon y reverse showi ng eharaete ristie
ora nge- re d to m aro on colo u rs.
Penieilli str ietly sim ple, usu all y bo rne upon stipes a ris ing di ree tly from the
su bst ratu m 0 1' from a d en se ba sal felt , eree t, seld om braneh ed , va ria ble in
length, eom mo nly less th a n 200 u it, but in sorne stra ins up to 300- 400 tuu ,
with a piees usu ally enla rged in a ves icle .
Con id ia l ehains lon g, loosely parallel 0 1' in eo m paet eolumns, ero wde d in
mos t spee ies and 01'ten tending to b reak away as erus ts in age.
:Illlid ill HIIIOo lh 01' delicutcly rou ghcn cd , clliptical lo Hu!>glo!>oHe 01 ' cvc n
ItlhuHl:, 1'1'0111 2.0 l o 2.5 {t lll in su me spcc ies to 4-.0 0 1' 5,0 {t ll l in oihcrs.
l' l' v
\,
lo
spccics
Page
rat her restrictedly upon most substrata, especially
P. implicatum series . . .. 82
lonias attaining a diameter at least of 20-30 mm in 14 days at room
tumperature (20-25C) .
1' , Conidial areas in pale blue-green shades, colony reverse in bright
oro nga-red or red shades, conidia globose to subglobose, in parallel
or divergent cha ins.
P. multicolor G.-M. et P. . . . . 84
' , Conidial areas commonly in deep blue-green shades, colony
rovarse in orange-brown or maroon shades, conidia broadly ellip tlcal, usually in compact columns.
P. implicatum Biourge . . .. 86
' , Conidial areas in yellow-green to gray -g reen shades.
aa. Colony reverse in orange-red (approaching brick-red) , conidia
strongly elliptical or pyriform .
P. sublateritium Biourge . . . . 88
bb. Colony reverse in greenish yellow shades .
1". Con idia small (less than 3 ~ m in diameter), smooth -walled or
nearly so.
aa. Conidia strongly elliptical or pyriform , smooth-walled.
P. ramusculum Bat ista et Maia . . . . 92
bb. Conidia globose to subglobose, with walls smooth or
nearly so.
P. aeneum Smith . . . . 90
2" . Conidia comparatively large, up to 4.5 ~ m in long axis ,
conspicuously roughened .
P. palmensis Ramrez et al. . . . . 94
4' . Conidial areas in green-black shades , colony reverse in bright
yellow shades, sulphur yellow pigment diffusing int o the surrounding agar, conid ia globose, conspicuously spinulose.
P. ardesiacum Novobranova . . . . 98
5' . Conid ial areas in gray -blue shades, colony reverse in yellow
shades, co nidia globose to subglobose, smooth-walled or nearly so.
P. ga//aicum Ramrez et al. . . . . 100
b . Colonies growing very rest rictedly, attaining a diameter not
surpassing 17 mm in 14 days upon Czapek's agar.
P. hispanicum Ramrez et al. . . .. 96
C olo nies upon C A (co lou r plat e 23, A and a) u su all y growi ng rather
restrictedl y , attaining a di amet er 01'20-25 mm in 14 days at room te m peratu re
(20- 25 OC ), but in sorne strains, as it is the case 01' stra in C BS 35 1.48 stud ied
here (colour plate 23, A a nd a) , spread ing ra the r rapidly , attain ing a di amet er
01' 50 mm in 14 d ays at room temperature , co n sisting 01' a com paratively thi ck
ba sal felt, 1.0-1. 5 mm d eep , close textu red and fairly tou gh , velvety, with
shallow radial furrow s; heavily spo ring in sorne stra ins, poorly in others,
almost lanat e but presenting a velvety appearance , 0 1' showing both cond itions
in th e sa me colony ; with gro wing m argin 1- 2 mm wide , pal e yellow to bri ght
ora nge in colou r; in sorne strains co nid ia l structures developing in locali zed
cen tral 0 1' su bcen tra l areas agains t a background 01' yellow to ora nge-red
j't'r!o
B
.:~\ ~ -:.::
(}
luWJJ.u.J
10
jrrn
"lr L
l...--....J
100 jrrn
R.
e,
VI'Wt;ltivc hyphae (co nsp icuous ly studde d with orange to orange-red gra n ules
wlu- n vicwe d under low magnifi cation ), in othe rs , heavily spo ri ng throu gh out
11 hlu c-gr een to deep bluish gray-gree n shades (S guy, PI. XXX , 443, 449);
I1 Xlll la l c limited to abundant, ye llow to pale ora nge; odo ur faint , suggesting
u iush ro orns ; colony revers e (colour piate 23 , a) in bri ght ora nge-red to burnt
Ie:lllla (Sguy , PI. XIII , 181 , 194) ; yellow to pa le ora nge-coloured pigm ent
t1 ifl'll sillg int o th e surraunding agar ; conid ial structu res abu ndantly produced
11 SClII 1C stra ins, sparingly in othe rs; peni cilli strictly simple (fig . 23, A), with
tilll'S 01' th e conid iophores m ostly arising directly from th e substratu m but
ol'l'as iona lly from aerial hyphae , usually unbranch ed , varying from short up to
lOO /U 11 in len gth by 2.0 -2 .5 /lm in diamet er, wi th walls smooth in so me
tra ins, gra n ula r in othe rs, or showi ng both cond itions in th e same strain, with
Ipiccs slightly enlarged or de finitely ves icula r in some stra ins, up to
i,O- :>. 5 /lm in di amcter ; phiali des in com pact wh orl s , usually 6 - 12 in the
vr-rt icil, in some strains m or e , mostly 8 - 10 /lm by 2.0- 3 .0 uiu , with apices
pnrall cl or di vergent ; conidia at first elliptical, becoming subglobose to
.UlI
d cli cat cly rou gh cn cd wh cn obsc rv ed 1I11<il:1' th c Iighl mi croscopc (lig . 2:1 , B) but
cons p icuous ly rou gh cn cd , a ppcarin g cc rc b rilorm wh cn ob scrvcd un d er th e
SEM (plate 4 , bott om : left) ; co n id ia l cha ins up to 100 ftllll on g , loosely parallcl
~ t an ~ ed (fig . 23, C ).
Colon ies upon C YA (colour plat e 23, B and b) spreadin g broadl y, a ttain in g
a di amet er of 60 m m in 14 d a ys at roo m tempcr a tu rc (20 - 25 OC ), essc nt ia lly
duplicating those grown upon C A in texture a n d gro ss colo ny appearancc , but
deepl y radiately furro wed ; ex uda te a b u nda n tly p roduced in bri ght orange
shades ; co lony reverse (co lour pia te 23, b) sim ila rly coloured in bright ora nge
shades; odo u r faint , suggesting mush rooms; bright yellow-colou rc d pi gment
di ffusing into the su rro u nd ing agar; penicilli as describcd a boye .
Colo n ies upon MEA (co lour plat e 23 , C a nd e), a ttain ing a di amet er of
30-45 mm in 14 d ays at ro om te m pe ratu re (20 - 25 OC ), a ppcaring velve ty to
subfloccose , com mo nly tufted in cen tral a re as in mi xed orange-red (fro m
hea vily e ncruste d hyphae) a nd blue- gr een sh ades (Sguy, PI. XXX , 443)
(fr om co ni d ia l struc tu res), with the former pred ominating in so rne strains, th e
latter in ot he rs, a nd cove re d by a n ove rgro wth of white vegeta tive hyphae in
sorne strains; growing m argin with hea vily e nc r usted orange-red hyphae
largely submerged , exte nd ing about 5 mm beyond th e ae r ial gro wt h; exudate
limited 01' lacking ; odo u r faint , suggesting mu shrooms; colony reverse (colour
piate 23, e) in bright orange-red colou rs (Sgu y, PI. XIII , 181); bright orangeyellow pi gment diffu sin g into th e surround ing agar; penicilli as described
a boye .
Sp ecies description is ce n te re d upon strain CES 35 1.48 = C M I 40, 574 = ATC C
10,471 = N R RL 2, 058 = QM 2,685 = IJFM 5040 , isolat ed from exposed textil es in
Florid a by Prof. W eston from H arva rd U niv ersity , Boston in 1946; also upon
stra in N R RL 2, 059 , isolat ed from a wat er pond in Brookl yn , N .Y . by Miss
Laura A. K olk fro m Brookl yn College, Brookl yn , N.Y . , in 1944; also upon
strain N R RL 2060 , isolat ed from ex posed cello phane in Florida , b y DI' L. White
in 1945.
Attempts to ob ta in th e typ e cu lture fail ed. Penicillium multic olor was isolated
or iginally from Ru ssian so il in 1915 by O .C . G r igo r ieva-Mano ilova a nd N .N .
Poradielova .
While it ca n not be ass u med with ce rta in ty th at th e stra ins assig ne d by Raper
an d T hom ( 1949) to P. multicolor G. -M et P . duplicat e in all det ail s th e strain
origin all y described by the au tho rs ofthis species, th er e is conside rable reason ,
accord ing to the forme r a u thors, for beli evin g th a t they must ha ve b as ed th eir
spec ies upon a for m similar to those co n side re d by them .
24 . Penicillium implicatum Biourge: fig. 24, colour plat e 24, and SEM
o O
O O
00 BO 000
O
O O O O
O O
O
00
O
liliili.wJ
10 jarn
C
)
1
}.j
"~\' '\
/
~,
jI\,
'"
R.
...
.7 ~
L-....:.J
100pm
\\
Penicil/ium imp /icatum Biourge - Monographie, La Cellule. 33: 278-280. 1923. See
also Ch. Thom , 'The Penicillia ' , pp. 210-211. 1930; also Raper and Thom, 'A
Manual of the Penicillia', pp. 201-203. 1949.
cha racter; stipes 01' th e co nid iophores sho rt , mostly under 100 ,un lon g by
2.0-2 .5 'LID in diaruc tcr , a rising usu ally from th e su bs tra tum in a den se sta nd,
occasionally from trail in g h yphae , with wa lls srnoo th 01' nearl y so (fig . 24, A) ;
phialides in cornpact clusters, m ostly 8 - 12 in th e ve rticil, ranging from 6.0 to
12 .tm by 2.0 -2.5 .tm; con idia com parativ ely small, elliptica l, 2.5-3 .0 .tm by
2.0- 2.5 uit) , m any subglobose, 2.5 -3 .0 utt, occasionalIy larger up to
4. 5- 5.0 tun, heavy-walIed , sm oo th 0 1' delicately rou ghened (fig . 24, B, and
SEM plate 4 , bottom : ri ght) ; con id ial chains in definitely compact to loose
colum ns up to 200 .tm 0 1' more in len gth (fig. 24, C) .
C olonies upon C YA (colour plat e 24, B and b) gro wing m or e rapidly th an
upon C A, a ttain ing a d iameter 01' 40 mm in 14 days at roo m temperature
(20-25 OC), deepl y radiat ely fur ro wed , otherwise duplicating in colony
characte ristics a nd det ails 01' m orphology, th ose gro wn upon CA ; reverse
(colou r plat e 24, b) in yellow-orange shades; sim ila rly coloured pigment
d iffusing into the surro und ing agar; penicilIi as desc ribe d aboye .
Colonies upon M EA (colour plate 24, C, a nd e) , attaining a di ameter 01'
25- 45 mm in 14 days at room temper ature (20-2 5 OC ) , plane , cons picuo us ly
zona te, with ce ntre umbon at e ; heavily sporing th rough out in bluish- gr ay
shades (Sguy, PI. XXXIV , 508); exudate a nd odo ur lacki n g; colony reverse
(colour plat e 24, c) in bright yelIow sha des; peni cilli as describcd aboye, with
conid ial chains formin g loose colum ns , with a tenden cy to form crus ts in old
colon ies; thin m argin surrou nded by a frin ge 01' su bme rge d m ycelia extend ing
abo ut 5 mm beyond th e aerial growth.
Sp ecies description is cente re d upon th e typ e strain CBS 232.38 = IJF M 5013,
strain C BS 337 .48 = IMI 40,5 78 = ATCC 10,453 = NRRL 2,054 = I Fa 6,098 = Q M
3,2 71, and numerous othe r strains possessin g esse ntially th e sa me cult ural and
m orphological characteristics .
The aboye description is drawn in broad eno ug h terms to incl ude a gro u p 01'
stra ins di ffering som ewhat in particul ar characteristics , but showing sufficient
sim ila rity in ge neral appearanc e and det ail s 01' m orphology to be cons ide red
toge the r. The species is com mo n in soil and cosmo polita n in distribution .
T ypi cal strains 01' P. implicatum show con idial areas in deep blue-g reen
colours upon alI su bstrata, with th e blue element pred om in ant. T hey
characteristically appear as rest ricted , hcavily spo ring, with reverse in ora nge brown to m aro on shades . H owever, occasional strai ns, othe rwise typical,
develop yelIow-gree n rather th an dee p blue-gr een shades in con id ial areas.
Sinc e th e colou r change fro m strain to strain is pro gressive a nd no satisfac to ry
line 01' separation ca n be drawn , recogn ition 01' a variet y covering th ese forms is
not con sidere d adv isa ble. It wilI be sufficiently to re cog nize su ch varia tio ns .
25 . P enicillium sublateritium Biou rge : fig. 25, colour plat e 25, and SEM
...
0 '
00 O
0
iJ..w.WJ.J.J
10 ).lm
O O
sS OOOOO
00 0 0 0 O
O
O O,
lrl~
~
100 jrrn
(1{.
com in g ellip ticul, with wa lls smooth or finely roughencd (Iig. 25 , B, a nd SEM
pla te 5, top: lcft ); eonid ia l cha ins up lo 100 {u n lon g, beeom in g la nglcd in age
(fig . 25 , C). Conid ial arcas showing gray shades in a ge.
Coloni es upon CYA (colo ur plate 25 , B and b ) growi ng more rapidly ,
attaining a di amet er of 35 mm in 14 da ys a t room temperature (20-25 OC ),
consisting of a c1ose-textured basal felt, velv ety in appearance, deeply rad iately
wr inkled, buckled in ce ntre in a crateriform pattern; heavily spo ring
throughout in blue-green shades (Sguy, PI. XXVIII , 412); und ula tin g thi n
margi n, white; colony rev erse (colour plate 25 , b) in cin namon shades; odou r
and exuda te lackin g; a m ber-colou red pigment diffusing into the surrounding
agar ; peni cilli as described aboye.
Colonies u pon MEA (colour plat e 25, C and e) attain ing a d iameter of
45 mm in 14 days at room temperature, loose-textured , velvety, plane , slight ly
ra ised in centre; margin evenly whi te; heavily sporing in deep artemisia gr ee n
shades (Sg uy, PI. XXXI, 462); odour arid exuda te lackin g; colony reverse
(colour plate 25 , c) in pale yellow to cinna mon shades; pale amber- coloured
pigment di ffu sing into th e surrounding agar; penicilli as described aboye, very
small, wit h short stipes a rising mostly from trailing hyph ae ; conid ial chains
loosely parallel to ta ngled .
Spe cies description is ce ntere d upon th e typ e strain C BS 267.29 = IM I
40 ,59 4 = ATCC 10 ,502 = NRRL 2,07 1 = IFO 6, 107 = QM 7652 = IJFM 5047 , isola ted
by Biourge in 1923.
This stra in appears to suffer actually from a vitamin deficien cy sinc e it gro ws
very restrictedly , sporing poorl y upon CA bu t not upo n CYA.
Colon ies upon CA (colour plat e 26, A and a ) growing slowly to moderately ,
attaining a diamet er varying from 22 to 40 mm in 14 days at room temperature
(20-2 5 OC ) , appearing lea thery , plane , slightly raised in ce ntral ar ea ; th in ,
fimbriate m a rgin, surrou nded by a fringe of submerged hyphae exte nd ing
about 2 mm beyond th e aerial growth ; sporing in blu ish gray-green shades
when isola ted , but very poorly sporing actually , espec ially in marginal a rc a s,
l.l.w.LuJ.LI
}1m
10
. ~!.
fR.,
( '~
l~m
' Colonies on Czapek agar growing slowly, very bluish gray-green, with a narrow,
slightly yellowish, fimbriate margin, velvety but somewhat heaped in the centre;
reverse dull yellow or developing a greenish east whieh might be termed bronze.'
Colon ies upon C YA (co lou r plat e 26, B a nd b) a tta in ing a diame ter of
35 mm in 14 days a t room temperature (20- 25 OC), velvety to leather y ,
deepl y fur ro wed in a radiat e pattern , buckled in the ce ntre; undulat in g
m argin , su rro unded by a narrow fringe of su bmerged hyph ae extend ing abo ut
1 m m beyond th e ae rial gro wth; poorl y spo ri ng, rc main ing persisten tly white
to pale yellow ish shade s in colou r; exudate abu ndant to limited in amo u nt, as
light am be r d roplets ; colony reverse (colou r pla te 26, b) in cinnamon shades;
simi la rly coloured pigm en t diffusin g in to th e surround ing agar; pe nici lli as
describe d aboye, with stipes short, arising fro m trail in g ae rial hyphae .
Colon ies upon M EA (colour plate 26, C a nd c) a ttaining a d iame ter of
50 mm in 14 days at room te m pe rature (20-25 OC ) , typi cally velvety, plane or
nearl y so, w ith centre slig htly raised , with tufts of whitc hyphae cove ring the
colony su rface in sector s, lendin g it a gr anular appearance; thin , irregul arl y
d issected m argin with a wide frin ge of su bmerged hyphae ex tending abo ut
4 m m beyond th e aerial growth; heavily sporing th rou gh ou t in blue-gr ay
shades (Sg uy, PI. XXXIII , 488); ex udate lacking ; odo ur lacking or
indefinite ; penicilli as described aboye, but with stipes mostly arising from the
su bs tratu m, a nd with con id ial chains loosely parallel. No coloured pi gm ent
diffusin g into th e surrou nd ing agar; colony reverse (colour plat e 26, c) in
cream shades .
Species description is centered upon th e typ e strain CBS 321.59 = CM [
68,225 = ATCC 18,308 = [F O 6,224 = Q M 7,290 , isolat ed by S. Ab e fro m J apanese
so il in 1956. The species is also represented by Ab e ' s stra ins FAT 123 a nd FAT
124, isolated from th e same source.
11
!J..wl.wJ.J
10)Jm
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! ,~
If
,-
"\ c
'
O OO O OO
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o C)
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. " D. ...
..: t
\1 ..
l-----I
100 jrrn
C)
0
O
f{.
I.(ar ; penicilli strictIy simple, arising from aeri al hyphae and ropes of hyphae
(lig . 27 , A) , with the stip es of th e conidiophores smooth walled , som etimes
Hc' platc , short , 15-2 5 tut: in length by 1. 5-2 .5 flm in diamet er, slightIy
1;lIlar gcd at the ap ex up to 3 .0 flm in di amet er; phialides in cro wde d clusters,
IIp lo 12 in th e vertici l, parallel or divergent , m ostIy 8. 0 - 12. 0 flm by
" . () - :~ . O tui , terminating in a long, tapering, narrowed neck ; conid ia pyriform
lo cllip tical with walls sm ooth or fine1y roughen ed (fig. 27 , R, and SEM plat e
,i , ce n tre: left) , 2.2 - 2 .8 flm by 1.0-1. 6 flm ; conid ial chains forming short ,
loosc!y parallcl to definite1y com pact colu m ns, bec oming tangled in age (fig.
0'7 , D).
Colonies upon C YA a tta ining a di amet er of 25- 30 mm (colour plat e 27 , R)
iu 14 days at room temperature (20- 25 OC ), similar in texture and colony
uppca ra nce to th ose grown upon CA , but appe a ring m or e gr anular ; very
poorl y sporing, rem aining persistentIy white to white ros y ; exudate and odour
lackin g; colony reverse (co lou r plat e 27 , b) in cin namo n shades ; pale a m be rcoloure d pigment diffusing into th e surrounding a ga r ; penicilli as des cribed
ahoye .
"
"
@I
~@
@i~ @ u....L.w
G/IJ~
10 um
B @O
~-
C~
j l'.L'"
).::L
\ \
l'
cj;0o
1I
I!
.,/
L--....J
J1m
'
"
"".
'
r-ll iptical whe n for me d and usu all y remaining so, altho ugh in sorne strains
bccorning ovate, 4.0 -4 .5 /lm by 3.0-3 .5 usi, cons picuo us ly rou gh ened with
thc ru gosity di spl ayed in an helicoidal pattern (fig. 28, B), appearing
n :rebriform whe n obse rve d under th e SE M (pla te 5, centre: ri gh t) ; conid ial
chains form ing lon g , well defined compact colu mns , up to 300 /l m lon g (fig .
28, C).
Colonies upon C YA (colour plate 28, B and b) spreadi ng broadly, attaining
a d iame ter of 48-50 mm in 14 days at room temperat ure (20-25 OC),
1ypically velve ty, radiately and deeply furrowed , wit h centre irregul arl y fold ed ;
(hin, eve nly m a rgin , with su bmerge d hyphae exte nd ing abo ut 1 m m beyond
(he ae rial gro wt h ; heavily sporing throu ghout in deep blue- green shades, wi th
the bl ue pre dominant (Sguy, PI. XXX, 448), especially in marg inal areas;
submarginal and central areas cove red by an ove rgrowth of white aerial
hyphae influen cing the colony appearance by obscuring the otherwise
abundant con id ial structures; odour lackin g ; exudate limited in amo u nt , in
pa le yellow droplets; yellow -coloured pigm ent d iffus ing int o the surrou nd ing
agar; colo ny reverse (colour pla te 28, b) in shades of ochre; peni cilli as
c' .
\ '
~l' J
Y1 (JJ1V
'-------'
100.um
.J{.
29, C) .
Colon ies upon CYA (co lou r pl at e 29, B a n d b) gro wing a littI e less
re strictedly than upon CA , a tta in in g a di ameter of 23-25 mm in 14 days at
room temperature (20- 25 OC ), co nsisti ng of a close -tex tu re d basal felt ,
velve ty, radiatcly furro wed and rais ed in th e colony ce n tre , sho wing a
depression in a crater ifor rn pattern ; h eav ily spo r ing throu gh out in th e sa me
dcep green sh ades as upon CA , but showing zo nes in yellow shade s; od our a nd
ex udatc lacking ; dark amber to orange-coloured pi gment diffusing into th e
su r rou n d in g a ga r, becoming d ark brown with a ge; colon y reverse (colour pl at e
29, b) in d a rk brown shades; penicilli as described above.
Colon ies upon MEA (co lour pl at e 29, C a n d e) a ttain ing a di amet cr of
26 mm in 14 d a ys at ro om te m pe rature (20- 25 OC), velve ty, zo nate, pl an e ,
with white , thin m a rgin surro u nde d by a fringe of su b merge d hyphae
exte n d ing about2 mm beyond th e ae r ial grow th; heavily sporing throu ghout
in deep blue-green sh ades, with th e blue predominating (Sguy, PI. XXX ,
447) ; ex u date a nd od our lacking; yellow-o ra n ge -colou re d pi gment diffu sin g
into th e su r rau n d ing aga r ; colony rev erse in yellow- orange shades (co lou r plate
29, e); penicilli as described aboye.
The sp ecies di agnosis is ce n te re d upon th e typ e strain IJFM 3223 = ATCC
38, 667=CBS 691.77 , isolat ed b y M .S. Ferrer from th e su rface of a Citrus
limonum L. fruit in M adrid , Spain , in 1975. It is a t present kn own on ly frorn
th e typ e species .
:W. Penicillium ardcsiacutn Novobra nova : Iig. :W, colo ur plat o 30 , and
SE M plat o 5 , (bottom: right).
oc)! (;)
-------- 10
~
Jlm
( ., )
C:~~,.,.
JI
'}ii;}
( ' ~ 1{
,
L--..l
100 urn
f./? .
Penicitlium ardesiacum Novobranova - Nov . Syst. Niz . Rast. 11: 225-234. 1974.
.3- 4.5 ,tm in dlameter. It is asslgned with the P. imp/icotum series. No related
pecies are known .
Type: The type strain N. 421 has been isolated from branches of Vitis vinifera L. in
ctober 1967 by T.1. Novobranova, in Kazachstan, Alma-Ata region, USSR.
(Translated from the Russian by Prof. L. Vilasl,
O ur notes follow :
C olonies upon C A (colour plate 30, A and a) gro wing rather restricted ly ,
a ua in ing a diamet er of 35 mm in 14 da ys at room temperature (20-2 5 OC),
vclvcty , with flat , thin margin, su rro u nded by a frin ge of su bme rge d hyphae
cxtcnd ing 3- 4 mm beyond th e ae rial growth; su bce ntra l and ce ntra l arca s
with shallow furrows in a radial pattern, with centre raised ; heavil y sporing
1hrou ghout in deep blue-gr een shades (Sg uy , PI. XXX, 446), espec ially in
marginal zones; showing an overgrowth ofwhite to pale N apl e yellow (Sgu y ,
1'1. XVIII , 270) ae rial hyphae obs curing th e otherwise abundantly produced
ro nid ial structu re s in central a nd su bce ntral arca s; odo u r and exudate lackin g ;
ro lony reverse in deep cad m iu m yellow shades (colour plat e 30, a); bright
yellow-co lou re d pi gm ent diffusing into th e surrou nd in g aga r ; penicilli strictly
sim ple (fig. 30, A), with stipe s of th e conid iopho res arising directly from th e
substra turn, with wall s deli cately rou ghened to coarsely granular, with apices
nlarged in a ve sicle up to 6 J-m in diamet er, u sually sho rt , less th an 75 J-m in
len gth by 3. 0- 4.5 J-m in diameter ; phialides com p aratively large , 10-14
crowde d into the verticil, 10-1 3 J-m by 3.5 - 4 .5 utu , terminating in a
narrowed short neck ; conid ia glob ose to su bglobose, cons picuo u sly spinu lose
(lig. 30, B, and SEM piate 5, bottom: right) , m ostl y 3.5- 4.0 J-m in diamet er;
conid ial ch ain s in definitely com pac t colu m ns (fig . 30, C ).
Colon ies upon CYA (colour piate 30, B and b) attaining a diameter of
40 mm in 14 days a t room temper ature (20- 25 OC) , velve ty, cons picuo us ly
zona te , deepIy sulca te radi ately with few wrink1es , with ce ntre irregul arl y
fold ed and slightly rais ed ; thin a nd undulating m argin with a frin ge of
su bmerg ed my celi a exte nd ing abo u t 1 mm beyond th e aeri al growth ; heavily
sporing throu ghout in deep blue-green (Sguy, PI. XXX , 448) to dark brown
shades, but con id ial fru ctifi cations ob scured in zone s by an ove rgrowth of
white ae rial hyphae , lending a su bla nate appe ara nce to th e colony, sho wing
light salmon-pink shad es in age ; od our faint , 'mouldy' ; exudate lacking or
limited in amount in sulph ur yellow droplets ; colony revers e (co lour plate 30,
b) in orange shades with a green cas t sho wing th rough; su lph ur yellowcoloure d pigment diffusin g into th e surro u nd ing a ga r; pen icilli as de scribed
aboye .
C olonies upon MEA (colour piate 30, C and c) atta ining a di ameter of
50 mm in 14 days at room temperature (20-25 OC) , velv et y , conspicuously
zonat e , showing some what floccose tufts of aeri al h yphae in central areas; thin
m argin, with a fringe ol' submerged h yphae exte nd ing up to 10 mm beyond th e
acria l grow th; hcavil y spo ring in ve ry dc c p blu c-grecn , nlmos t bl nck shndcs
(S gu y , PI. XXX , 446) in m argin al a m i su hcc n tmi arcas, a nd in a rtc m isia
gree n shades (Sguy, PI. XXIX , 425) in ce ntra l a rcas; ex udatc a nd odou r
lack ing; colony rever se (co lou r plat e 30, e) in o ra nge sha dcs; no colou red
pigme nt di ffusin g in to th e su rraund ing agar ; pen icilli as described aboye .
The species di agn osis is cente rcd up on th e type st rain CB S 497 .7 :3 = VKM
F-1 ,7 49 = C M I 174 ,71 9 = A T C C 24, 719 = IJ FM 7070 , isolat ed from vine -shoo ts of
Vitis vinifera L. in Kaza ch stan , Alm a-Ata region , U SS R , by T. I. Novobranova , in 1967 .
el
Berenguer: fig . 3 1, co lo u r
u..w.u.w
10jJm
O O
0 0 0 000 O
00 00
00
00 8 0 0 0
00 O O
O O
f'
tV, (J! . e
~:L1: V ~
L.--...J
100jJm
iR
co nsisting 01' a tou gh , den se , closc-tc x tu red basal felt , with m argin al arca planc,
ce n tra l ami s u bce n tra l arca s ru iscd a m i irrcgul url y Iold cd ; whit c , c n tirc , thi n
m a rgin surround ed by a Iringc 01' su b ruc rgc d hyph a c ex (cnd ing a bo u t 2 mm
beyond th e a er ia l gro w th ; co lo ny su r lace a p pcar ing vel vety, with ce ntre
g-ran u la r , m ainly cluc lo a th in ovc rg rowth of w hite ae r ia l hypha e ; poorly
spo r ing in pal e brow n shade s in m argin al zo nes a nd in ye llowish colou rs in
ce n tra l an cl su bce n tral arca s ; ex ucla te a n cl odo u r la cking ; reverse (colour pl ate
:)1, a) in pal e o ra nge shades in ce n tre ancl colou rl ess in m a rgin o Penicilli
com rno nly str icty sim ple, seldom wi th a n occas ional branch which bea rs a
verticil of phialides re tain ing a lways its m onoverticillate character; th e stipes of
th e co n icliophores short , u su all y less th an 50 tm long by 2.0- 3.0 tm in
d ia mete r (fig. 31, A) , wi th wall s smooth, hyal in e, a r ising e ither from th e
su b stratu m 0 1' a s short branch es fro m aeria l trailin g h yphae , with a p ices
enla rgecl in a ves icle up to 5 tm in wiclth ; phialides closely pa ck ed in pa rall el
clu sters , m ostl y 6 - 10 per verticil , ranging fro m 7.0 to 12 tm in length by
2.5 -4 .0 tm in cliamet er , with short n arrow n eck s ; con iclia glo bose to
su bglobose, smooth-wall ecl 0 1' nearl y so when obse rvecl uncl er th e light
mi cro scope , but deli cately ro ughene d when obse rve cl u n cler th e SE M (fig . 31,
B, a ncl SEM pl ate 6, top : left) , measuring 2.0 -3 .0 tm in di amet er ; co ni clial
ch a in s forrning loose colu m ns 0 1' tanglecl, up to 150 tm long (fig . 31, C) .
Colo n ies upon C Y A (co lo ur pl a te 31, B a n d b ) growing a little m ore rapicll y
th an upon CA, a ttain ing a di ame ter of 35 mm in 14 clays a t roo m temperature
(20- 25 OC ) , tou gh , velvety, close textu recl, zo nate, cleeply ra di atel y wrinkled
with numerous furrow s , with ce nt re ra isecl a nd irregul arly folclecl in a
cra ter ifo rrn p attern ; eve n m argin su rro u n de d b y a frin ge of su b mergecl hypha e
ex te n di ng a bou t 2 mm beyoncl th e ae ria l growth ; h eavil y spo ri ng za nes in
blue- green shacles (Sguy, PI. XXX , 436), poo rly spor ing zo nes showing
su lph ur yello w co lours (Sg uy , PI. XX , 286) , m ainl y clue to th e ye llow
enc ru stecl hypha e ; ex uclate in small, yello w clrops ; oclour la cking ; colony
reverse (co lo ur pl at e 31, b) in ye llow-o range sh acles ; su lphur yello w-co lou re cl
pi gment cliffu sing into th e su rro u ncling agar. Penicilli as clescribed aboye .
Colo nies upon M EA (co lo ur pl ate 31, C a n cl c) a ttain ing a di amet er of
27 mm in 14 cla ys at ro om tempera ture (20 - 25 OC ), co ns isting of a leathe r -like
b a sal felt formecl of yello w enc rus tecl vegeta tive h yph ae, pl ane , with ce ntre
sligh tly rais ecl a n cl clepressecl, za nate ; thin m argin with subm erged yellow
encru stecl h ypha e ex te nd ing 3- 4 m m beyoncl th e ae rial grow th ; h eavil y
sporing in blue-gray shacles (S g uy, PI. XXX , 449); odou r a n d ex u clate
la cking ; colon y reverse (co lo ur pl at e 31, e) in cleep su lph u r ye llow colo u rs ;
sim ilarly co lo urecl pi gment di ffu sing into th e su r ro u ndi ng agar; pe n icilli as
clescribecl a bove .
Penicillium gallaicum a ppears to stancl b etween species incluclecl within the P.
imp licatum se r ies a n cl th e P. decumbens se ries . On accou nt of th e restri ct ecl
g ro w rh o n m ost m edi a it suc ms lo lil m ost sa lisfa <: tlll'ily withi n (he ormc r
ser ies, bu t Oll acco u n t 0 1' ih c sho rt co n id io pho rcs a ris illg" mo stl y fro rn nct wo r ks
01' tr ailing h yphae it could als o be assignablc to th e P. decumbens series.
Species diagnosis is centered on the type st rain I.f F M 5597 = A T C C 42,232 , isola ted
fro m the ai r 01' M ad rid , Sp ain , in 1979 . It is a t prcscnt kn own on ly from the
typ e st rain .
Colonies growing slowly, u su ally restrietedly , su rfaee appear ing ve lve ty 01'
slightly la n a te , eons isting 01' a net work 01' trai ling a nd inter woven vcgeta tive
hyphae , rangi ng fro m com pa ra tively loose textu re d in so rne species to close
textured, tou gh, dense m yceli al felt s in othe rs .
Penieilli str ictly sim ple , co ns istcntly small , usually typi call y borne on short
stipes a r ising frorn trai lin g , 0 1' interwoven ae r ial hyphae , seldom from the
su bs tratum .
Con id ia small , usu ally somewhat ellip tica l, a bo u t 2.0-3. 0 .,m in th e lon g
ax is, smooth wa llcd in m ost spcc ics, delicately raughened in others.
K ey to th e spec ies
2. Colonies appearing velvety or with surface slightly lanate ; stipes borne
prima rily as short branches from loosely trailing or compacted vegeta tive
hyphae.
P. decumbens series
a. Colon ies loose-textured , w ith margin usually thin and gene rally
consisting of a loose network of interlacing hyphae bearing short
stipes .
1' . Conidial areas in Iight gray-green shades w ith reverse in yellow
drab shades upon CA but becoming cherry red upon MEA
P. chermesinum Biourge
2' . Conidial areas in dull blue -green shades with reverse usually
colourless upon all med ia.
P. decumbens Thom
b. Colon ies c1ose-textured, tough , almost leathery, restricted , with
margin compact but showing occasionally stolon-l ike hyphae .
1'. Vegetative mycelium yellow, gene rally characterizing the colony
even in age , sporu lating slightly upon CA.
P. citreo-viride Biourge
2'. Vegetative mycelium white , often characterizing the colony when
yo ung , conidial areas deve loping blue-green shades .
Page
.... 102
. .. . 106
. . . . 105
. . . . 108
111
117
128
129
131
120
122
124
113
115
118
0 0 0 0
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00 o 0 0
Bo o o
() 0 0 o o
0
w....J.LwJ
10jJm
e
l....----..J
100jJm
fR.,
Penicillium decumbens Thom - Bull. Bur. Anim . Ind. U.S. Dep. Agr ic. 118: 71 .
1910. See also Thom , 'The Penicillia', pp. 197-198. 1930; also Raper and Thom,
'A Manual of the Penicillia' , pp . 209-212. 1949.
Coloni es upon CA (co lour plate 32, A and a) growing slowly, attammg a
diameter of20- 30 mm in 14 days at room temperature (20-2 5 OC), velve ty in
some strains, with a tenden cy to develop a white mycelial overgro wth in
othe rs, and in stil1 othe rs almo st lanat e, deep, u p to 1- 2 mm , bu t al1
cha rac te rized by loosely interwoven a nd tr ailing hyphae from whi ch arise short
stipes, plane, very thin m argin with sub m erged hyphae ext ending largely
beyond th e ae rial growth (abou t 2-4 mm) ; heavily spo ring in grayish gr een to
olivaceous shades (Sg uy , PI. XXI, 307); exudate lackin g or lim ited in
a mount; odo u r distincti ve , fragrant , su ggesting soap perfumes; colon y revers e
(colour plat e 32, a) almost colou rle ss, with a green ca st showing through ; no
coloured pigment diffusin g into th e surro u nd ing agar; peni cil1i strictly sim ple
(fig. :~2, A), with the stip e 01' the conid iophores sho rt , :>0- 100 JJ.1T1 by
2.0 -2.5 JJ.1T1 , with apiccs slightly c nla rged in a vesiclc IIp lo :U ) JJ.IIl in
diarnct cr , with walls smooth 01' deli cat cly rough cn cd , borne al su cccssivc nodos
upon trailing hyphae, which in marginal arcas grow stolo n -like struc tu res
along th e substratum ; phialides usually in compact clusters u p to 12-15 in the
verticil , 7.0-9 .0 IJ-m by 2.0 -2 .5 IJ-m, sometimes borne at two immediately
a djacen t lev els ; co n id ia ellip tical to su b globose , 2.0-2. 5 IJ-m in long axis ,
occ asionally up to 3.0 IJ-m , smooth walled (fig. 32, 13) to delicately roughened
when observed under th e SEM (plate 6, top : right) ; conidial chain s producing
short , loo sel y parallel colu m n s, up to 100 IJ-m long (fig . 32, C).
Colon ie s upon CYA (colour plate 32, B a nd b) a tt a in in g a diamet er of
25- 30 mm in 14 days at room temperature (20-25 OC ), velvety , radiately
fur rowed with ce n tre fold ed in a somewhat crate r ifo rm pattern ; thin margin
with a fringe of su bmerged h yphae exte n d ing less th an 1 mm be yond th e aerial
growth , developing a white m ycelial ov ergrowth in cen tral a nd su bce n tra l
a rcas , othe rwise duplicating the co lon ies grown upon C A in cu ltu ra l charac te risti cs ; ex u date abundantly produced, crystal clear, as colo urless droplets;
odour fra grant a s described a boye; colo n y rev erse (col our plate 32, b) in
yellowish cream shades in m argin al a reas a nd in deep gree n co lou rs sh owing
throu gh in th e ce n tra l arca s. P enicilli as described aboy e.
C olo n ies o n MEA (colour pi a te 32, C a n d c) a ttain in g a di amet er of
35-50 mm in 14 d a ys a t ro om temperature (20- 25 OC ) , typically velve ty ,
pl ane ; heavily spo rin g in dark olive-g re en sh ades (S gu y , PI. XXIX , 433);
thin m argin irre gul arly di ssect ed , surro u n de d by a frin ge of su b merged h ypha e
ex te nd ing a bou t 3- 4 mm beyond th e aerial gro w th ; exu da te a nd odou r
la cking ; co lo ny re verse (colo ur plate 32, e) colo u rless , with a greenish cast
sho wing throu gh ; n o colou re d pigment diffu sin g into th e surro u nd ing agar .
Penicilli as described aboye ; co n id ia l chains forming loos el y p arallel co lu m n s
up to 250 IJ-m long .
Spe cies d escription is cen te red upon strain ATCC 10,436 = C BS 318.48 = NR R L
742 = IF O 6,093 = C M I 39,755 = Q M 1,920 = IJFM 7068, isol ated by Prof.
Sherbakokk , University of Tennessee , Knoxville , T enne ssee , USA, also on
strain C BS 258.33 = N R RL 2,044 = IJFM 5003, which represents th e sp eci es satisfa ctorily but tends to produ ce co lon ies somew ha t de ep er a nd of looser texture
th an th e former strain , a n d a lso upon m any other strain s th at present th e same
general m orpholo gy .
The species a ppear s to be com mon, a nd worldwide in di stribution , a nd h as
b een isolated primarily from so il a nd det eriorating military eq u ip men t in
tropical co u n tr ies .
000
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}-1m
Synonymies
Penicillium citreo-nigrum Dierckx - Ann. Soco Sci. Bruxelles. 25: 86. 1901 : see
also Biourge's Monographie, La Cellule. 33: 273-274. 1923.
.r-:
00 O 00
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~t
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'
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-1
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100 )Jm
11 0
"'Ill
' - .- 1 - -
el' work ers may hav e ba sed species descrip tion s upon va riants 01' st rai ns
kn own to re prcscnt differcnt aspects of the sarne spec ies, which is th e case
IC fivc spec ies listed above as syno nyms of P. citreo -viride.
~O . , Cl ~
(~p
O () .:\:::l
00 O O
O
O0 ,':'"':: 0
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. O ()O
.'
00 <
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100}Jm
enic lium fe/lutanum Biourge - Monographie, La Cellule. 33: 262-264. 1923. See
also Ch. Thom , 'The Penicillia', pp. 198-199. 1930; also Raper and Thom, ' A
Manual of the Penicillia', pp . 212-215. 1949.
ynonymies
Penici/lium phaeo-janthine/lum Biourge - Monographie, La Cellule. 33: 289-290.
1923.
Colon ies upon CA (co lour plate 35, A and a) growing rather res tricted ly,
a ttaining a diameter of 20- 30 mm in 14 days at ro om te mpera tu re
(20- 25 OC ), co ns isting of a tough , close- te xtu red basal fel t, 100- 200 utt: deep ,
wi th m argin ofte n surrou nde d by a na rrow zon e ofpredo m inan tly su bmerged,
fu nicu lose , sto lon-like h yphae , radiately furrowed , with concen trical wrinkles ,
n ar rowly za nate, cave red by a mycelia l overgrowth of snow-white ae rial
h yphae in flue ncing greatly th e colony a ppeara nce; poorl y spo r ing in sorne
st rains, only in m arginal areas, with centre rem aining persistently white,
conid ial a reas in light blue- gr een shades (Sguy, PI. XX , 449); ex udate as
colou rless d ropl ets em be d de d in th e myceIial feIt ; odour not pronounced ,
rath er pleasant; colon y reverse (co lour pl at e 35, a) in pal e cream to flesh
colou rs or in light vinaceous shades , in sorne strains greenish co loured; penicilli
stric tly sim ple, wi th a n occas ional b ranch (fig . 35, A), co m parativeIy large ,
wi th sho r t stipes, usu all y 50- 100 utt: in length by 2.0-2 .5 /l m in diamete r,
with wall s smooth , ar ising from interlacing, trailing or creep ing h yphae ,
co m mo nly enla rge d at th e a pe x in a vesicle up to 4.0- 5. 0 /lm in dia me te r;
ph ialides in compact clusters 8 - 12 in th e ve rt icil, compara tiveIy la rge , m ostly
10 -1 5/lm lon g b y 2.0- 4.0 tut: in di amet er, with fairly long tapered neck ;
co nid ia elliptica l to subglobos e, mos tly a bo ut 2.5 - 3 .O /lm in long axis, smooth
or cons picuo us ly ro ug he ne d (fig. 35, B, a nd SEM pla te 6, botto m : left);
conid ia l chains form ing loosely paralleI or d iverge nt, poorl y defin ed colu m ns
up to 100 /lm in length (fig . 35, C).
Colo nies u pon C YA (colour plate 35, B and b) attaining a diameter of
35 mm in 14 days at room temperature (20 -25 OC ), simi lar in tex ture and
cultu ral cha racte ristics to those grown u pon CA, bu t m ore deep ly ra d iateIy
fu rrowed, with centre raised a nd depressed in a craterifor m pattern, an d very
poorl y sporing, re maining persisten tly white in colou r ; undul ating m argin
su rrou nded by a narrow fri ng e of su bme rg ed hyph ae ex te nding abou t 1 mm
beyond the aeria l growth ; colony su rface con spic uo u siy zanat e ; exudate
a b u ndan tly p roduced in co lou rless dro ps; odour lacking; colony reverse
(colo u r plate 35, b) in cream shades; pal e amber-colou red pigmen t d iffus ing
into the surrou nd ing agar ; penicilli esse ntia lly as described a boye .
Colon ies u po n MEA (colo ur plat e 35, C a nd c) attain ing a d ia meter of
40 mm in 14 days at room temperat u re (20-25 OC ), ty pically veIvety ,
sp ccics 01' Penicillium, individu al strains sho w a p prec inb lc vuriati on in gross
co lo n y a ppea rancc , in th e re lat ivo a bu nd n ncc 01' spo r ulutio n , a m i in lcsser
d cgrcc in the Iincr d etails 01' m orphology . T h is is proba bl y th e rcaso n w hy
ea rlier workers m a y hav e b a sed spe cies d c scripti on s up on va ria nts or str a ins
now kn own to represen : diff erent as pe cts 01' th e sa m c species, wh ich is thc ca se
of th e Ii ve sp ecies listed above as syn on ym s 01' P. citreo -oiride.
0
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100).lm
Synonymies
Penicillium phaeo-janthinellum Biourge - Monographie, La Cellule . 33: 289-290.
1923.
Colonies u pon CA (co lour plate 35, A and a) grow ing ra th er restricte dly,
attaining a d ia meter of 20-30 mm in 14 days at room temperatu re
(20- 25 OC ), consisting of a tough, close-textured basal felt, 100- 200 /l m de ep,
with margin oft en surrounded by a narrow zone of pred om ina ntl y submerged,
fu niculose , sto lon- like h yphae , rad iately furrowed , wit h conce n tr ical wrinkles ,
narrowly zonate, covered by a mycelia l overgrowth of snow-white aerial
h yphae in flue ncing greatly th e co lony appearance ; poorl y sporing in sorne
strains, only in marginal areas , wit h centre remaining persistently white ,
conidial areas in light blue-gree n shades (Sguy , Pi. XX , 449) ; ex udate as
colou rless droplets embedded in the m ycelial felt ; odour no t pronounced,
rat he r pleasant; colon y reverse (co lou r plate 35 , a) in pale cr eam to flesh
colou rs or in ligh t vinaceou s shades , in sorne strains greenis h colou red; penicilli
strictly simple, with an occasional branch (fig . 35 , A), com pa ratively large ,
wi th short stipes, usu all y 50-100 /lm in length by 2.0-2.5 /l m in diameter,
wit h wa lls smooth , arising from interlac ing , traili n g or creep ing h yph ae ,
com m only enla rged at the apex in a vesicle up to 4.0-5 .0 p.u: in d iameter;
phiali des in compact clus ters 8- 12 in the verticil, comparative ly large , mostly
10-1 5 /l m long by 2.0- 4 .0 /lm in d ia m eter, with fairly lon g tapered neck ;
conid ia elliptical to subglobose, mos tly abo ut 2.5-3 .0 /l m in long axis , smooth
or conspicuously roughened (fig . 35, B, and SEM plate 6, bottom : left);
con idial chains form ing loosely parallel or divergent , poorl y defined co lumns
u p to 100 /l m in length (fig . 35, C).
Colon ies upon CYA (colour plat e 35, B a nd b) attaini ng a di amet er of
35 mm in 14 days at room temperature (20-25 OC), sim ila r in texture and
cult u ral ch aracte ristics to those grown upon CA, but more deep ly radi a tely
furrowed, with cen tre raised and depress ed in a crate riform pattern , and very
poorly sporing , remaining persistently white in colou r; undulating margin
surrou nde d by a narrow fringe of submerged hyphae extend ing about 1 mm
be yond the aerial growth; colony surface conspicuously zanate; ex ud ate
ab u ndantly p ro d uced in co lourless drops; odo ur lacking ; colon y reverse
(co lour plate 35, b) in cream shades ; pale amber-coloured pigment diffusing
in to the surrou nding agar ; penicill i essen tia lly as described aboye .
C olonies u pon M EA (colour plat e 35, C and c) attaining a d iamet er of
40 mm in 14 d ays at room temperature (20-2 5 OC ), typicall y velv ety,
36 , Penicillium roseo-purpureum Dierckx: fig. 36, coIour plate :i6, nud ~'mM
:::::..-----
0 0
o ~ oB oo9
00008
O 00 0
O 00
1~
O
O
O
e ~,- ~
.JJ: ~
~ .S- ...y
~
100 jrm
~
f{.
11'1
Penicillium roseo-purpureum Dierckx - Ann. Soco Sci. Bruxelles. 25: 86. 1901. See
also Biourge 's Monographie, La Cellule. 33: 317-319. 1923; ibid. 36: 452. 1925;
also Ch. Thom, 'The Penicillia' , p. 181 . 1930; also Raper and Thom, 'A Manual of
the Penicillia' , pp. 218-220. 1949.
Synonymies
Penici/lium carmino-violaceum Dierckx - Ann. Soco Sci. Bruxelles. 25: 86. 1901 .
Biourge's Monographie. La Cellule. 33: 281-282. 1923.
Penicillium internum Morotkovsky - Bul. Sci. Rec. Biol. Univ. Kiev. 2: 78-79.
1936.
Penicillium [Citromvcesl sanguifluus Sopp - Monographie, pp. 115-117. 1912.
Colo nies upon CA (co lour plat e 36, A and a) grow ing restrict edl y , att ai n ing
a d iameter of 25- 30 mm in 14 days a t room temperature (20-25 OC ),
gradually raised towa rds th e cen tre , velvety, with shallow wrinkl es in m argin al
zones, wh ich are ab ru p t; close-t extured basal felt , formed of slende r
interwoven h yphae ; very poorl y sporing, pe rs iste n tly white, grayish wh ite to
flesh or pal e pink shades in areas whc re eonidial structu res are m ost ab u ndan t;
exud a te lacking or limited in sorne strains, whi le a bu ndan t in others , in pin k to
vin aceou s red shades ; odo u r faint , indefinite or lacking; eolony rcver se (eolou r
pla te 36, a) white , rosy, orange-red, ora nge or te rra co tta -colo ured, dep ending
on th e strain; pale vinaceous red -colou red pi gm ent d iffus ing slow ly into the
surro u nd ing agar; penicilli strictly sim ple (fig . 36, A) , with sho rt stipes arising
usuall y as sh ort branch es from tr ailing a nd in terl acing ae ria l h yphae , or
occasionally fro m submerged h yphae , u su ally less th an 50 /l m in len gth by
1.5 - 2.0/lm in d iamete r , wit h wa lls smooth ; ph ialides, few in numbcr or
crowdcd , up to 8- 10 in th e verticil, mostl y 6-8 /lm in len gth by 1.5-2 .0 /lm in
di am eter; co n id ia at first ellip tica l, th en su bg lobo se to globose, co m paratively
small , 2.0-2 .2 /lm in di amet er, wit h wall s del ica tely rou gh en ed whe n observed
under the light microscop e (fig. 36, B) , bu t co ns picuo usly roughen ed in a
cere b rifor m pattern wh en observed under th e SEM (pla te 6, bottorn: ri ght) ;
conid ia l chains usu ally sho rt , bu t a litt le lon ger , up to 100/lm , in heavier
spo ru la ting strains (fig. 36, C) .
Colon ies upon CYA (co lou r pIate 36, B a nd b) a tt a in ing a d ia me te r of
30 m m in 14 d ays at room temper ature (20 - 25 OC ) , dupl icating in tex ture and
colony ap pearance th ose grow n up on C Y A, but m ore deepl y rad ia tely
furro wed a nd more int en sely colou red in pinkish sha des; ex udate and odo ur
lackin g ; poo rly spo ring; vinac eo us red-colou red pigm ent di ffusing into the
surro u ndi ng m ediu m ; colony rever se (co lour pla te 36, b) in salmo n pink to
b rown sien na shades; penicilli essentia lly as described a boye .
Colonies on MEA (co lour pla te 36, C a nd c) grow ing res tr icte d ly , at tai ni ng
a di a m et er of 35 m m in 14 d ays at room temperature (20- 25 OC ), velve ty,
now whit c in colour , with thin murgin surrou ndcd by a fringc 01' ycllowish
1I11 lllllc l'ged hyphae cxt c nd iug a ho ut !i IIIln bcyond th c ac ria l gro wth; colony
111'l :\Ce co vc rcd by a m yccli al ovc rgrow th , lendin g a lan nt c a ppeara nce (o th e
('olo ny; poorl y spo ring , but hcavi cr th an on th c abo ve subs tratc ; exu dare a nd
ndou r lncking or ind efi nit e ; colony reverso (colour plate :'36, e) in yellow, dull
III:I nf.(c-brown lo deep brown shades, dep ending on th c strain ; pal e ambc rI'o lolll'e <l pigment diffu sing into the su rrou nd ing agar, becorning hrown in
th c colo nics in terspace. Pen icilli esse ntia lly as described aboye .
'I'hc spec ies description is ce ntcred on the typ e strain c ns 266.29 = NR RI.
~, ()( 'l = \TC C 10,492 = C M I 40,573 = IJFM 5029, isolated by Prof. Ph . Biourge in
1~ ' '2 ~ I , a nd other stra ins having sim ilar m orphology, com b ine d with R aper ami
'l'llllln's original not es (1949) .
ludividual strains are kn own to vary a ppre ciably in pattern such as
vuria tions in colony margin , often showing aggregation of aerial hyphae int o
I'II\'CS or prostrate fas cicles, dep ending on th e su bs tra tu m a nd other
r-uvi ro n me n t a l factors.
'l'he species a ppears to be world-wide in d istribution , but not abu nda nt in
II II III I'C . R epresentative stra in s of P. roseo -purpureum hav e occa sionally bccn
slIlatcd from soil and from det eri or ating military eq u ipment in tr opi cal a nd
Id ,1 ro pical cou ntrie s.
:17 . Penicillium glauco-lanosum Chalabuda: fig. 37, colou r platc 37, and
00 cP OO
o
O O
0 0 o
;)7
"-../
Oo OO O
B O0 0 O
00
O
O O O 0
O
lu.u1J.wJ
10 jim
11~~1}
~
100}lm
Icl'i1l: h yph uc , pl anc , a ppcaring gra nula r; thin m nrgin su rro u ndc d by a Irin gc
suln ucrged h yph ac extc nd ing ahou t 4 11I11l bcyond th e ac ria l grow th ; hcavily
i1H'inl-{ throu ghout in gr ay sh adcs (Sgu y , PI. XXXIII , 49 3); exud ate a nd
udu u r lnckin g ; colony revcrse (colour plate 3 7, e) in very pale cre a m shade s;
u ub cr-co lou red pigm ent difTusin g into th e su rro u nd ing agar ; penicilli
llNNl ' lI 1 ially as described aboy e .
SllC :cics di agnosis is cen tc re d upon th e typ e strain CB S 224 .66 = BK M Fj - \ T CC 18 ,47 8 =IJ FM 5027, isolated from Ukranian soil , in th e enviro n s
lit' Kic w, U SSR , by T.V . Chalabuda in 194 9 .
A d ose rel ationship between P. glauco-lanosum Chalabuda and th e P.
1t'/'/II/1!Jm .l' se ries is not presumed here , but th ey are con sidered toge the r
1Il'IIl arily as a m atter 01' conve n ience. They do , how ever, have ce rta in features
11 1'11111mon , particularly th e tough , close ly wo ven felt offine h yphae , th e com prm uively slow-gro wing colonies , espec ially on CA, and th e sho rt stipes,
uu rs t ly arisin g from tr ailing 01' pros trat e cree ping hyphae.
111'
~I Il ,
f~
---
(~
o
O O
00
B O O
O O O O 00
0 0
0 0
00
00
0
CJ 0
R.
~~
e
100
jrrn
terilc hyphu c , plane , nppca ri ng gra nular ; (hin ma rgin SlIITO U IH lc d by a Iringu
JI' suhmcrged hyph ac extcnd ing abo ut '1' II1l1l bcyond thc aerial g-rowlh; hcavily
p"l'ing" rhrou gh out in gray sha dcs (Sguy, PI. X XXIII , 493); exudate a nd
HlI lIll' lacki ng ; colony reverse (colou r platc 37 , c) in very pale cream shades;
uubcr -coloure d pigm cnt di ffusin g int o th e surrou ncling agar ; pen icilli
(' HMI ' 1I1ially as dcscrib ed aboye .
Spccics di a gn osis is cen tere d u pon the type strain CBS 224 .66 = BKM FH;I I -ATCC 18 ,47 8=IJFM 5027, isola ted frorn U krania n soil, in the environs
I JI' Kic w, U SSR , by T .V . C halabuda in 1949.
\ close relat ion sh ip bet ween P. glauco-lanosum C hala buda and the P.
ircnmbcns series is not presu me d here , but they are considered togethe r
primuri ly as a m atter 01' convenie nce . They do, however , h ave certain fea tures
111 co tum on , particul arl y the tou gh , closely woven felt 01' fine h yphae , th e cornpnrurively slow-gro wing colonies, especially on CA, and th e short sti pes,
nll lslly arising from trailing 01' prostr ate cree ping hyphae .
(~
o
O
O O 10
O O O 00
0 0
00
0 0 0
0 0
O ()
R.,
C) .
Colo nies upon C YA (co lou r plat e 38, R and b) gro wing res tr icted ly, a ttain ing a di amet er 01' 21 mm in 14 d ays at ro om temper ature (20-25 OC), deepl y
ra d iately fur row ed, with ce n tre rai sed in a cra te r iform pattern ; eve nly m argin
su r ro u nde d b y a narrow fringe 01' su bmerged hyph ae exte nd ing abo u t 1 mm
beyond th e aeri al gro wth; colony surface poorl y spo ring, remaining
pe rsiste n tly whi te to wh ite ros y , velvet y in a ppeara nce ; odo ur faint , indefinite ;
exudate lackin g; colony reverse (colo ur plat e 38, b) in cin namo n shades; pale
am be r-co loured pi gm ent diffusin g into th e surrounding ag ar; penicilli
esse ntially as described a bo ve .
Colon ies upon M EA (co lo u r plat e 38, C a nd c) growing restrict edl y ,
at ta ining a di amet er 01' 25 mm in 14 d ays a t room temperature (20- 25 OC),
velve ty, plane, sligh tly co nvex ; white , wide, evenly m argin ; heavily sporing
th rou gh out in blue-gr een shades (Sguy, PI. XXXI , 462); ex ud ate lackin g;
odour fragra nt, aromatic; colon y rever se (colou r piate 38, e) in light yellow
shades; pal e a m be r-co lou re d pi gm ent di ffusin g into the surrou nd ing aga r ;
penicilli esse nt ia lly as described a boye, but with th e sho rt stipes arising m ostl y
from trailing rop es 01' hyphae ( Fig . 38, C ).
Sp ecies description is ce nte re d up on th e type strain C BS 763.68 = IJ FM 5036,
isolat ed from rizosphere soil 01' Brassica campestris, var. toria, in Lu cknow , Indi a ,
by j .N . Rai and K. Wadhwani , in 1968.
39. Penicillium bilaiae Chalabuda : fig. 39, colour plat e 39 , and SEM
plate 7, (cen tre: left) .
7~~
00000
000 O
O
000
O
BO
0
O
O
O
i.J.J..ulillJ.J
10
}1m
R.
>V
Ce ".(
"t\[ '111
L--J
100
jrm
Penicil/ium bilaiae Chalabuda - Nov . Syst. Herb . Inst. Bot. Komarovii, Aead. Sei.
USSR. 6: 165. 1950. See also M.M. Kulik, 'A Compilation of Deseriptions of New
Penicil/ium Species'. Agre. Handbook No. 351. Agrie. Res. Ser. U.S. Dep.
Agrie. p. 20. 1968.
cnla rged al. api ces in a veside up lo :L5 P.III in di um ctcr; phi alid es usually few
in number, 2-6 in the verticil, 01' crowded in clusters , 9-14 in thc vcrt icil,
7-9 p.m by 2.5- :L5 J.tm, terrninating in a co nsp icu ously tapercd , sho rt,
narrowed neck ; con id ia usually g!obose to subglobose , apparcntly smooth
when obs erved under the light microscope (fig . 39, B) , but de!icatc1y
roughened when obs ervabl e under th e SEM (plate 7, centre: left), 2.0 - 3.0 J.tm
in diameter; conid ial chains forming more 01' less loosely parallel colu m ns, up
to 150 J.tm long (fig. 39, C).
Colonies upon CYA (colour plate 39 , B and b), attaining a diameter al'
35 mm in 14 days at room temperature (20-25 OC ), consisting al' a closetextured bas al felt , velv ety , deeply radiately sulc ate , with numerous furrows ,
with centre raised and irregularIy folded in a crateriform pattern; sporing
abundantIy in pale blue-green shades (Sguy , PI. XXX , 444) ; exudate
abundant, in amber colou red drops; odour lacking; amber coloured pigment
diffusing into th e surrounding ag ar ; colony revers e (colour piate 39, b) in dark
brown shades; undulating margin surrounded by a narrow frin ge al'
subm erged hyphae exte nd ing 2-3 mm beyond th e aeri al growth; peni cilli
essentially as described aboye.
Colonies on MEA (colour piat e 39, C and c) attaining a diameter al' 30 mm
in 14 days at room temperature (20-25 OC ), velvety in appearance, plan e,
slightly raised in central areas; colony surface cave red by a my celial
overgrowth a l' int erwoven white ae rial hyphae , and rapes a l' hyphae en crusted
with dark purple-red crystals , lending a pinkish appearance to the colony;
poorIy sporing in pal e blue-green shad es (Sguy , PI. XXX , 444); thin ,
undulating margin , surrounded by a fringe al' purple enc ru sted subme rged
hyphae ext ending about 5 mm beyond th e ae rial growth; odour and exudate
lacking; colony revers e (colour pIat e 39, e) in dark purple shad es; arnbe rcoloured to vinace ous red pigment diffusing into the surrounding a ga r;
peni cilli essentia lly as described aboye .
The spec ies description is centere d upon th e type spec ics C BS 221. 66 = BKM
F -854 = IJFM 5025, isolated from Ukranian soil in the enviro ns a l' Ki ev, USSR ,
by T .V. Chalabuda, in 1949.
A clase relationship between P. bilaiae Chalabuda and th e P. decumbens seri es
is not presumed here . However , th ey are con sidere d tog ether, primarily as a
m atter al' conve nience . They do , how ever, show certa in characte ristics in
com mo n, such as the slow-growing colon ies, espec ially upon CA, and th e short
stipes, usuall y arising from tr ailing 01' prostrate cree ping hyphae , reminding
thos e species included within th e P. f ellutanum sub- series.
~ 00 0
o O
0 0
O ~O OO
000
0
lw.L!.u.J..U
10
)Jm
fR .
~ i-ll
I--J
100 ).1m
'oniclllium kurssanovii Chalabuda - Nov. Syst. Herb. Inst . Bot. Komarovii, Acad .
Sci. USSR . 6: 164. 1950. See also M.M. Kulik , ' A Compilation of Descriptions of
New Penici/lium Species'. Agric. Handbook No. 351. Agric. Res. Ser. U.S. Dep.
A gric . p. 48. 1968.
2. 0 -:~ . O
:::::::--------
::.;::-oo"}
.,-,
~\" ',
.-. ,
~;
,.:,
'<i
-,.J
i.wWJ.uJ
10
jrrn
'1flJ1J
100
)Jm
small , Iew in number , 2 - ;> in th e verticil , 01' somutime c ro wdc d IIp lo 12 pcr
whorl , comruonly 5.0 -9 .0 ~ 1lI by 2.0 -:LO ~ nl , tcrtuinut ing in a short , rap er ed,
narrowed neck ; conid ia at lirst ellipso id a l, bccomi ng subglobosc, 2.5 -3 .5 ~m
in d iamet er , conspicuously echinulatc (lig . 41, B, and SEM plat o 7, bottom:
left) ; conid ial chains short , up to 30 ~m long, forming loosc1y d ivergen t
01' tangled colu m ns (fig . 41, C) .
Colonies upon CYA (colour pIat e 41 , B a nd b) a ttaining a di ameter of
30 mm in 14 days at room temperature (20 -25 OC ), cons isting of a tough,
close -textured basal felt, de eply and radiately wrinkled ; margin en tire ; poorly
sporing, with a colon y su rface remaining persistently white rosy ; ex uda te
lacking ; odour faint, ind efinite ; colon y reverse (colour pl at e 41 , b) in ligh t
cinnamon shade s; amber- coloured pigment diffusin g into th e surrounding
agar ; peni cilli esse ntially as des cribed aboye .
Colonies upon MEA (colour plate 41 , C and c) attaining a d iamet er of
40 mm in 14 days at room temperature (20 -25 OC ), conspicuously zonate,
ve1vet y to lanate , due to a loose network of fine ae rial white h yphae ; m argin
thin , with a wide fringe of submerged hyphae ex te nd ing about 3 mm beyond
th e aerial gro wth ; exud ate and odour lacking; sporing ab u nd a n tly in blu e-gray
shades (Sguy, PI. XXXIV , 50S); co n id ial structures somewhat obs cured in
the colo ny centre by the myce1ia l overgrowth of interwoven floccose hyphae ;
colony reverse (colour plate 41 , c) in crea m sh ades ; pale amber -coloured
pigment diffusing into the surrou nd ing a ga r; penicilli essen tially as described
aboye .
The sp ecies description is ce ntere d upon strain CBS 222.66 = BKM
F -S56 = IJFM 5024 , isolated from Ukranian soil in th e enviro n s of Ki ev , USSR ,
by T.V . Chal abuda, in 1949.
The sp ecies as originally described, and a s observed in ou r cu lt u res, agrees
satisfactorily in all respects, excep t that it has become somc wha t altered in
cultu ral aspect, and predominantly steril e upon CA and CYA, after 30 years of
laboratory cu ltivation .
Although a close re lationship between P. cmereo-atrum Chalabuda and the P.
decumbens series is not presumed here, th ey are con sidere d together, primarily
as a matter of convenience . They do , however , show certain characteristics in
com m on , su ch as th e com para tive1y slow-growing colon ies upon most
substrata , th e short , slende r stipes , arising from trailing hyphae, su ggesting
those species of Penicillium included within th e P. f ellutanum sub-series.
E) ... @
ttZ(\ ~
~ ~
~ ~
': ' ~.;."
/ ~
-!~; ~ Yit9
':::,~. _
(Jill)
',,,
B ....
.~
~ @
LwJlwJ
jrrn
10
.~ ~ .
..
r~
;\
L------J
100 ).1m
cum margine asqualiter a hyphis subm ersis 2 mm lat ltudlne formatur; lenlter
sporulantes, primum albas, dainde vlrido pallido colorantur; odor abest; axsudatio
adest, succineo; reverso pallido fusco vel salmoneo; cum colore succineo fusco in
medio diffluente; neque ascomata neque sclerotia visa sunt; penicilli pleraque
monoverticillati, stipitibus erectis, brevis aut mediocriter longis , plurimum e hyphis
reptabundis exoriuntur, plerumque tenus 100-200 Lm longitudine per 2.0-2.5Lm in
diametro, cum apicibus tenus 3-4Lm inflatibus; phialides numerosae, usque 8-12
per verticillum, plerumque 7.5-10 Lm longitudine per 2.5-3.5 Lm in diametro;
conidia globosa, 3-5 Lm in diametro, pariete aspere; in catenis compactis tenus
25-50 Lm longis.
Coloniae in agaro Czapekii cum extracto levedinis, post duas hebdomadas ad
20-25C, 55-60 mm in diametro attingunt, velutinae, radiatae plicatae, albidae vel
griseo viridae; leniter sporulantes; odor et exsudatio absunt; reverso fu seo in
centrum et virido fusco in marginem; cum colore fusco atro in medio diffluente;
neque ascomata neque sclerotia visa sunt.
Coloniae in agaro hordeaceo celerior quam in agaro Czapekii crescunt, post duas
hebdomadas ad 20-25C, velutinae, planae, margine plerumque a hyphis
submersis formatur; copiosae sporulantes, superficiebus conidiorum griseo
viridibus; exsudatio et odor absunt; neque ascomata neque sclerotia visa sunt.
Reverso flavido.
Typus cultura cas 351.51 ex Driza sativa L., Japoniae, a 1. Miyake isolata est
anno 1940.
Miyake's original diagnosis follows:
'Colon y growth of this fungus is restricted , in a radial pattern; attarnmq a
diameter of 2.5-3.0 cm in 14 days. With central area raised and somewhat
irregularly folded, but with the rest of the colony surface remaining smooth and flat.
The raised part is cracked and lanate . The colony margin is 0.5-1.0 mm wide; the
colony shows yellow colours at the obverse as well as at the reverse. The
conidiophores are 100-200 Lm by 3.0-3.5Lm; penicilli are strictly monoverticillate;
phialides are crowded in c1usters at the apex of the conidiophores, 7.5-8.0 Lm by
3.0-3.5 Lm. Conidia are somewhat globose, 2 .0-2.5 Lm in diameter, and forming
chains 25-50 Lm long .'
(Translated from the Japanese by Miss Masae Yamamoto) .
close-tc xturc d b asal fclt, su ggc sting (hose specics includcd within th c P.
f ellutanum su b -se ries .
~~
OO O 1~
pm
00 0 0
O BOa
O o o
:
, J.". iJ/
00
'\
O
O
-----
"" , !7
i.' '
c
. 7;~'<:
L.......-....J
100 jJm
al
4:~,
ddi calcly rou gh en ed wh en ob se rve d under th e SEM (pl ate 8, top : left) ;
l'llllidial ch a in s formin g loosel y parall el co lu rn n s (li g. 43, C ) .
C olon ies upon CYA (co lou r plate 43, B and b) growing faster than those
Kro wn upon CA, attaining a di amet er 01' 30-35 mm in 14 da ys a t room
u-mp crature (20-25 OC), co ns isti n g 01' a similarly tou gh, close-tex tu re d basal
rc'll , with colo ny surface r emaining persistently white ; velve ty , stro ng ly and
II<-cpl y radiately furrowed , with ce n tre rai sed and irregul arly folded in a
rr. uc rifor rn pattern ; eve n ly, undulatin g m argin , surrounded by a narrow
rl'ingc 01' submerged hyphae ex te n d ing 1 mm beyond th e ae r ial growth; po orl y
spo ri ng ; ex u d a te and odour la cking ; colo ny reverse (colour pl ate 43, b) in de ep
snlmo n-pin k to cinn a m o n shades; no coloured pi gm ent diffusing into the
surro u nd in g aga r; penicilli essen tially as d escribed aboye .
Colon ies upon MEA (co lou r pl at e 43, C and e) growin g restrictedly ,
a lla in ing a diamet er 01' 30 mm in 14 days a t ro om temperature (20-2 5 OC ),
ru ns isting 01' a d ense network 01' trailing a n d close ly interwov en ae r ial hypha e
rove ring th e e n tire colon y surface, lending it a sublanate a ppearance; thin ,
uu dul ating margin su r rou n ded by a n a rrow fringe 01' su b merged hyphae
r-xtend ing 1 mm b eyond th e ae r ia l gro wth; sp oring abundantly throu ghout in
pale blue- gra y sh ades (S guy , PI. XXX , 445-450); ex u date a n d odo ur
l.icking ; n o co lou re d pi gment diffusing into th e surrou nd ing a gar; colony
rcverse (col our pl at e 43, c) in cin namon shades. P enicilli essent ia lly as
dcscrib ed above .
Penicillium gerundense a p pears m ost close ly rel ated with sp ecies in cluded
within th e P. decumbens ser ies on account 01' th e sho rt stipes, com mo nly a rising
Irorn trailing hyphae , th e velu tinous a p pearance a nd slow growth 01' its colo n ies
lHI most su bs trata .
The species di a gn osis is ce n tere d on th e typ e strain IJFM 5967 = ATCC 42,234,
isolat ed from th e ai r, Madrid , Sp ain, 1977 . It is a t present kn own o nly fro m
th e typ e strain .
l.lJ.J.l.1.JJ..
10 jsrn
0000
0 0 0
00
00 0 0
0 0 0
~ ~~
L-..-.....J
100
jrrn
at
ce ntral area rai sed in a cra te riform pattern ; vel ve ty , with eve nly , white n arrow
m argin , 1 mm wide , surrounded by a narrow frin ge of su b me rged hyphae
ex te nd ing a bo u t 1 mm beyond th e aerial gro wth; colo ny surface at first white ,
lat er becoming yellow ish in non-sporin g areas and deep blue- gr een (S gu y, PI.
XXX, 44 6, 447) in marginal areas wh ere co nid ial structu re s a re abundantly
produ ced ; ex udate ab u ndan t to limited in a mo u n t, in yellow d ropl ets ; lacking
od ou r; colo ny rev erse (colour plate 44, a) in bri ght orange shades ; yellowora nge-co lo u re d pi gm ent diffusing into th e surro u nd ing agar; penicilli strictly
sim ple , with sho r t stipes, less th an 50 p.m in length by 1.5-2.0 p.m in di amet er,
smooth wa lled, with a p ices not enla rged in a ves icle , usu all y arising fro m
trailing hyphae; phialides bottle shape d, few in number , 2- 3 in th e verticil
(fig. 44, A), usually 6.0 -11 .0 p.m b y 2.0 - 3.0 tuu, terminating in a sho rt ,
tap er ed neck ; co nid ia globos e to subg lobose , smooth wa lled or nearl y so (S EM
plate 8 , top : right), m ostl y 2.5- 3.0 utt: in di amet er; con id ial chains forming
short, di vergent colu m ns .
Colon ies up on C YA (co lour plat e 44, B a nd b) a tta ining a di amet er of
45 mm in 14 d ays at ro om temper ature , du plicating in texture a nd cultu ral
charac teristics th ose grown up on C A , but sulcated with numerous and deep er
1:11
rud ial Iurrow s , co nspicuously zona tc nnd henvier spo riug , es peci ally in
ma rg inal a rcas in dcep blu e-g rccn shad<:s (S guy, PI. XXX , 447 ); cxud a tc
ubu ndan tly produccd in ycllow -orangc dropl cts; odou r lacking; colo ny reve rso
(colou r plato 44, b) in bri ght yellow -orangc shadc s; bri ght yellow- coloured
piglllent difu sing into th e surrounding ag ar; pcnicilli esse n tia lly as dcscribcd
a boye .
C olonies upon MEA (colour plate 44 , C and c) growin g m ore rapidly than
IIpon th e a boye substrata, attainin g a di amet er 01' 50 mm in 14 days at 1'00111
icmperature (20- 25 OC ), co ns isting 01' a tou gh , c1 ose-t extured basal Ielt ,
vclvet y to sublanate, slightly raised in ce n tra l a reas; thin , eve n margin ,
sur rou nd ed b y a frin ge of submerged hyphae exte nd ing about 3- 4 mm beyond
1he ae ria l growth; colon y surface remaining persistently white, due to th c
mycelial overg ro wth 01' fin e , close ly interwo ven ae ria l hyphae a nd th e pOOl'
sporula tio n up on th is substratum ; colony reverse (colour piat e 44, c) in pale
ycllow-o ra nge shades ; no colo u re d pi gment diffu sin g into th e surro u nd ing
"gar ; penicilli esse ntially a s d escribed aboy e.
Penicillium alicantinum a ppears most closely relat ed to species included within
lite P. decumbens series, on accou nt 01' th e slow-gro wing colonies, th e velv cty
appearance , a nd th e short stipes arising a s short branches from tr ailing
hyph ae .
The species diagn osi s is ce n te re d upon th e typ e strain IJ FM 7026 = AT C C
42,236, isolat ed from th e air, M adrid , Sp ain , 1978. It is at present kn own only
Irorn th e typ e strain.
;:::::::-----
~;\~ ~n
8 (;;,
i:~." ,:,:
'F:'
~r-~JJ$ f.R. .
1~ ~ Y
.i
(fig. 45, E , and SE M plate 8, ce ntre : left) ; conid ial chains forming sho rt, more
or less parallel colu m ns (fig. 45, C) .
Colon ies upon C YA (colour plate 45, B and b) a tt ainin g a di a m eter of
25 mm in 14 days a t roo m temperature (20-2 5 OC ), duplicating in tex ture and
cult ural cha racte ristics th ose grown on C A, but with better spo ru la tion; deepl y
radiat ely furrowed in m arginal and su bmarg inal zone s, with centre depressed ;
conid ial areas showing pale pink shades; ex udate abu nda n tly p roduced , in
crysta l c1ear, colourless drop s; odo ur pronounced , ' mould y ' ; colony reverse
(colour plate 45 , b) in reddish brown shades ; no coloured pigment d iffusin g
into the su rro u nd ing agar; penicilli esse ntially as described aboye.
C olonies upon M EA (colour plate 45, C and c) growing ve ry restrictedl y,
a ttai ning a di amet er of 15- 20 mm in 14 days at room temperature
(20- 25 OC ), velvety, plane, looser textured th an aboye; white, eve nly m argin,
su rro u nde d by a fringe of su bmerged hyphae extend ing abo u t 1 mm beyond
th e ae rial gr owth ; heavily spo ring throu gh ou t in deep gray-gre en, olive gree n,
or artem isia gre en shades (Sgu y , PI. XXIX , 421, 426, 428); exudate and
odour lacking; colony reverse (colou r plat e 45, c) in yellowish shades with a
gr een ca st sh owing through ; no coloure d pigment diffusing int o th e
Page
133
136
148
1:11
2'. Onlv achinulata, globosa conidia producsd .
aa. Colony reverse eolourless upon MEA.
.. .. 152
.. .. 150
.. .. 134
.... 139
.... 145
Synonym
Citromyces griseus Sopp -
Co lon ies up on CA (colour plat e 46, A and a) gro wing rather restrictedl y ,
attaining a diameter of 25 mm in 14 days at room temperature (20-25 OC),
deeply velvety to floccose in appeara nce, 1 mm or mor e thi ck , con sisting of a
tou gh , close -tex tured basal felt , covered by a thin mycelial ove rg row th of white
slender hyphae, deeply radi ately fur rowed and rai sed in colony centre,
t(;'.~j
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sIron gly pulling a part th e substratum and wit h colony surface cracked in the
ce ntre in a crateriform pattern; poorly sporing, re maining persistently whitc;
ex uda re and odour la cking; colony reverse (colo ur plate 46 , a) in ligh t yellow
shade s; penicilli str ictly simple (fig . 46 , A), with short stip es, mo stly 25 p.m 01"
lcss in len gth by 1.2 - 1.8 u ra , smooth walled , arising from aerial hyphae ,
occa sionally ir regul arly branched, showing some tim es one or two seco ndary
pcni cilli on the same st ip e; phialides u su ally d ivergen t, in sm all whorls of 6-8
in n umber, co mparatively small, about 5.0 -7 .0 tut: by 1.5- 2. 5 utt, bottle
shap ed , term inating in a short-pointed neck; conidia globose, about
'2 .0- 2.5 p.m in d iamet er with wa lls co nspicuously ro ughened (fig. 46, B, and
SEM pIate 8 , centre : right) ; co nidial chains tangled (fig . 46, C ) .
Colonies u po n CYA (colour pIate 46 , B and b) attaining a diamete r o'
:30 mm in 14 days at room temperature (20 -25 OC ) , dupli catin g in tex tu re
those grown upon CA, but deeply sulcated wit h numero u s rad ial furrows ,
with centre ra ised in a crat eriform pat tern ; u ndulatin g margin en tire; poorly
spo ring, with colony su rface remain ing pe rsisten tly white ; exudat e a bu nd an tly
pr oduced in topaz yellow d ropl ct s; a mhcr coloure d pigm ent dilu sin g inlo th c
su rro u nd ing agar ; co\ony reverso (coIour plat o 16, b) in c in narnon shades;
peni cilli cssc ntially as dcscribed ahoy e .
CoIon ies upon MEA (colour plat e 46 , C and e) gro wing less restrictcdIy than
up on th e aboye su bs trata, attaining a diarnet er of 40 mm in 14 days at room
temperature (20 - 25 OC), deeply floccose, plane , zonat e , with ce ntre urnbo nate ; colony surface cove re d by a my ce1ial ove rgro wth of close1y int erwoven slender aerial h yphae giving a powdery appe arance to th e coIony;
poorly sp or in g through out , with colony su rface remaining persistently white;
thi n m argin surra u nde d by a wide fri nge of su bme rge d hyphae extending
abou t 5 m m beyond th e ae rial gro wth; exudate and odo u r lacking; colony
reverse (coIour plat e 46, e) colourless to pale cream shades; pale arn be rcoloured pigm ent diffusin g into th e surrou nd ing aga r; penicilli esse ntially as
described aboye .
T he spe cies di agn osis is based upon cult ura l stud ies of strain CBS
367.48 = CMI 40 ,2 28= ATCC 1l,257=NRRL 1,748= QM 1,962=IJFM 5038 ,
isolated by P ra f. E .M . Gi Ibe rt , from H onduras soil, in 1937, a nd on G ilman
and Abbott's original description .
Stra ins show ing cultural a nd morphological charactcristics of thi s spec ies a re
frequently isolated fro m so il. (C olour plate 233 presents a representati ve strain
of P. restrictum, describcd by Kinoshita et al. as P. restrictum va r. kuensanii.) T hey
have a tenden cy to become sterile or nearly so afte r several tr ansfers on
arti ficial su bs trata .
2.5 - 4-.0 .UII in clia me tc r, smoo th wall cd , with upices enla rge d in a ves iclc up tu
6.0 Jl.m in di ameter: pcni cilli usu ally strictly sim ple , with a n occasiona l branch
(fig . 47 , A), com pa ra tivcly largc ; phi alidcs bo ttl e shapcd , mostl y in clus tc rs uf
6 - 14 e1eme nts in th e ve rticil, usually 10-1 2 Jl.m by 3.0 - 4. 0 Jl.m , terminati n g
in a sho rt, narrowed , tapered neck ; co nid ia at first subg lobose, becoming
globose, 4.0 -6 .0 Jl.m in d iameter, conspicuo us ly coa rse ly echi n ula te (fig. 4 7, B
and SEM plat e 8, bott om: left ), dark brown coloured; co nid ial chains in
loa se colu m ns 0 1' tan gled (fig . 47 , C).
Colon ies upon C YA (colour plat e 47, B a nd b) grow ing rapidly and
exte nd ing broadl y , attai n ing a diameter 01' 50 mm in 14 days at room
temperature (20- 25 OC), co ns isting a l' a close -text ured basal felt, cove re d by a
m ycelial ove rg rowth 01' loosely interwoven aerial hyphae , lendi ng a lanate
appe arance to th e colony surface; rad iat ely wrinkled, with cen tre irregul arly
fold ed ; thin m argin surro u nde d by a fringe 01' su bmerged hyphae exte nding
wide ly, abou t 4 mm beyond the ae rial growth; sparingly sporing , especially in
marginal a reas, re maining persistentl y wh ite; ex udate and odo ur lacking ;
colony rever se (colour plat e 47 , b) in pale N aple yellow shades (Sguy, PI.
XIV , 200); no coloured pigm ent d iffusi ng int o th e surro u nd ing agar; peni cilli
esse ntially a s described aboye , bu t with longer stipes aris ing directly from th e
subs tra tu m a nd also fro m the net work a l' floccose ae r ial hyphae ; con id ial
cha ins forming m ore 0 1' less com pact colu m n s.
Colonies u pon MEA (colour plate 47, C and c) spreadi ng bro adl y and
lu xu riantl y , attain ing a d ia met er 01' 65 mm in 14 days at room temperature ,
cons isting a l' a looser textured basal feIt, cavered by a mycelial ove rg ro wth 01'
floccose ae rial hyph ae , lending a lana te appeara nce to th e colony; thin and
wide margin, surro u nded by a frin ge a l' su bmerge d hyphae ex tend ing abo ut
5 m m beyond the aerial gro wth; heavily spori ng, espec ially in cen tra l and
su bce nt ral a reas in gray-gree n to brownish gree n sha des (Sguy, PI. XXV ,
373, 37 1) becom ing fusco us in age; odour lacking ; exuda te limited , in
colourless d roplets , enmes he d in the m ycelial felt ; no coloure d pi gm ent
d iffusin g in to the surro u nd ing agar; colony reverse (colour plate 4 7, c) in pale
yellow shades; peni cilli essen tia lly as desc ribe d aboye .
T he species d iag nos is is centered u pon cultu ra l stud ies 01' th e typ e strain CBS
3 10 .63 = C M I 99, 468 = ATCC 14,941 = IJ FM 503 4, isolated from a lod gepole pine
Dou glas-fir ( Pseudotsuga taxifolia) soil sa m ple collected in R avilli Cou nty ,
M ontan a , USA, by D I' M . C hristense n during th e su m me r 01' 1957.
Penicill ium montanense closely resembl es P. fuscu m (Sopp) R aper et T ho m.
H owever , P. montanense di ffer s from P. Juscu m in a number 01' mi cr oscopi c
characteristics, notably in its larger peni cilli , a nd its pOOl' grow th upon CA. O n
th e other h and , despit e its pred ominantly sim ple habit , P. montanense shows an
occasional character 01' the di varicat e peni cilli , which, in combination with the
dark bro wn colou r and th e stro ng ec hin ulation 01' th e conidia, a re rem in isce nt
11 1' P. meliuii T ho rn 01' the P. nigricnns se ries in th e Asytum cu -icn-idi varlcutu subsec tion ( R apc r and T ho m, 1!W J).
1B. l'enicillium airo -oirens Sm ith: lig. 4B, colour pla to 'lB, ami S EM plato
B, (bo llo m : right).
":'' ::.
~.~ \
?i::"'
L.wWwJ
10
).1m
tLttvif
l.-..-...J
).1m
100
Penicillium etro-virens Smith - Trans. Brit. Mycol. Soc o46: 331-337. 1963. See also
M.M. Kulik , ' A Compilation of Descript ions of New Penicillium Species' . Agr ic.
Handbook No . 351 . Agric . Res. Ser. U.S. Dep. Agric . p. 19. 1968.
Synonym
Penicillium decumbens Thom, varo atro -virens Abe - J . Gen. Appl. Microblol .,
Tokyo. 2: 70-71. 1956 (without a Latin diagnosis).
nnmcl y th c slow -growin g co lo n ics , IIH~ vclv cty a p pt:a ra nce , a m i lh e sripc
nrising
1'1'0111
l!) .
~;9$_'~
QJ!
t t1J f':-
t::;p
(@
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10 jirn
.K
Ji1
~
100 jrrn
Synonym
Citromyces fuscus Sopp - In Monographie der Pilzgruppe Penicil/ium mit
besonderer Bercksichtigung der in Norwegen gefundenen Arten. Videnskapselskapets Skrifter. 1. Mat.-Naturv. Klasse No . 11 . pp. 120-122. 1912. See also
Ch. Thom, 'The Penicillia', p. 180. 1930.
locali zcd a rc a s , showin g' sh allow radi al lurrows in narrow sub margina l arca s,
co ve red by a nctwork 01' stc r ilc ac ria l hyph ac , sho wing' sorn e ro pinc ss in ce n tra l
a reas; thin rnargin su rroundcd by a [rin gc 01' suhmcrged h ypha c cx ie nd ing
a bou t 1-2 mm bey ond th e acrial gro w th ; po orly spo r ing, with co lo ny su rface
remaining per sist entIy w h ite in strain s long maint ained under artifici al
cu lt ivation, but m edium spo r ing throu gh out , hea vier in m arginal a nd su b m arginal a reas, a t fir st olive gray (S guy, PI. XXIX , 427), becoming d eep
gra yish to b rown (Sguy, PI. XXIX , 433) in a ge , in recen t isol at e s; exud a te
and od our la ckin g; pale amber pi gment slo wly diffusing into th e surro u nd in g
a gar ; colo ny reverse (c olo ur pl ate 49, a ) in p al e cream sh ade s; penicilli var ia ble
(fig . 49, A ) , ran gin g fro rn fra gmentary to str ictIy sim ple to irregularly
branch ed (s uggesting th e Asymmetrica-divaricat a ), with two 01' m ore m etulalike cells borne terminall y 0 1' a t low er se p ta, with th e stipes o f th e co n id iop hores
variable , a r isin g m ostI y fro m ae ri a l h ypha e a nd u su all y not exceedi n g
100 tm in length b y 1.5- 2.5 tm in di amet er, se p ta te, with wall s smooth 01'
deli cately rou gh en ed ; phi alides di vergent , in small clusters up to 6 e1ements in
th e ve rticil, frequentI y 2- 3, m ostIy 7-1 3 tm by 2.5 -3 .0 tui , te rm in a ting in a
co m parative ly lon g , narrowed , tapered neck ; co n id ia globose, a t first co lo u rle ss , becoming olive brown when m ature , co a rsely ech in u la te with prominent
dark tu bercle s (fig . 49, B, a n d SE M pl ate 9, top : left ) , m ostIy 3.5-5. 0 tutv , 0 1'
up to 7.0 tm in di amet er; con id ia l chains tangled up to 50- 100 tm in length
(fig. 49, C).
Colo nies upon C YA (colour pl at e 49, B and b ) gro w ing m ore r apidly than
upon CA , a tta in in g a di amet er al' 30 -35 mm in 14 days a t room temperature
(20- 25 OC), similar in texture and cu ltu ral ch aract eristi cs to th ose grown upon
C A, but with ropiness m ore ev ide n t th roughout , plane , 1 mm deep in th e
ce n tre; thin m argin surrounded by a fringe a l' su bmerged hypha e ex te nd in g
about 1 mm beyond th e ae r ia l growth; very poorly spor ing, with su rface
r emaining persist ently white ; odo ur and exu date lacking; pale a m be r -co lo u re d
pi gment diffusing into th e su rrou n d ing aga r ; co lo ny reverse (co lo u r pl at e 49,
b) in pale crea m shades; penicilli as de scribed a boye.
C olo n ies upon M EA (co lour pl at e 49, C and c) sp read in g broadly , a ttain ing
a diamet er al' 55 mm in 14 d a ys a t room te m pe rature (20- 25 OC ), looser in
texture , co m parative ly thin , vel ve ty in a ppearance, with co lony su r face
charac te r ize d b y tr ailin g a nd interwoven ae r ia l h yphae; th in, eve nly m argin ,
surrou nded by a wide frin ge a l' sub merged hyphae ex te nd ing m ore than
10 mm beyond th e aeria l growth ; heavily spor in g throughou t in d ark mouse
gray shades (Sguy, PI. XXXV , 513) 0 1' oli ve- green co lours (Sguy, PI. XXI,
301); ex udate a n d odour la cking ; n o colo ured pi gment diffusing in to th e
surro u nd in g agar; colony rev erse (colour pIate 49, e) in reddish brown to olive
gre en shades; penicilli essentiall y as described aboye but with the stipes al' the
co n id iopho re s somewhat longer th an upon th e a boye substrata a nd m ore
III 'qu elllly b ranchcd in th e mnnn cr 01' thc Asyuu uctri cn-edivnricnru.
spc c ics di a gnosis is cc n tc rcd II pon 1he sI rui liS e li S 147.45 :1:J1.4B = C M I 39, 747 = A T C C 10,447 - NI~I{ 7'21 .. 11"0 6, 09 7 ..
' 1'111:
Ind (: IIS
IJ FM
QM
:)0 15
1,92 5 ,
Hnlll l ecl rom T exas soil by Pr of. M .B . Morrow, frorn th e Univcrsity o f T exas,
11
111:10 .
'1'111: co r rcc t placern ent of thi s species is still op en to question , T he
IlI Hi~ II I1 I C II I lo
n. Prnicillium
(1))
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Baghdadi's original diagnos is is as follows :
'Colonies upon Czapek's agar atta ining a diameter of 40 mm in 12 days , closs
textured , w rinkled, velvety to floccose , w ith c1avate, closely interwoven hyphae in
marginal areas, reminding c1eistothecia initials; nonsporulating areas in dirty white
shades; sporulating areas and reverse in gray-green colou rs; exudate limited in
amount, colourless or lacking.
The conidiophores arise from aerial hyphae, smooth walled or nearly so, 23-35
.tm by 3.3 .tm , sometimes up to 150 .tm in length; with apices enlarged in a vesicle
up to 3.5 .tm in diamete r; ph ialides bottle shaped, in c1usters numbering 6-8
elements in the verticil, 6-8 .tm by 3 .tm. Conidia globose, delicately roughened , 3
.tm in diameter.
Colonies upon malt extract agar attaining a diameter of 40 mm , close textured ,
plane, velvety, in lead gray shades, with centre white; colony reverse in darker
shades tha n upon Czapek's agar; heavily sporing throughout the colony surface .
Colonies upon corn meal agar attaining a diameter of 40 mm, c1ose-textured ,
velvety, wrinkled, in blue-g ray shades; exudate abundantly produced , in pale yellow
shades . The species is assigned to t he Monoverticillata stricta section. No closelv
related species are known . Several strains have been isolated from Syrian soil in the
environs of lrna and Darai, Damascus region , and in the environs of Es-Suveida , EsSuveida region (Svr ia), in August 1964, by the author.
The type culture T 16 has been deposited w ith the Cult ure Collection of the M.B.
Lomonossov University, Moscow, USSR , and with the Culture Collection of the
University of Syria , Damascus .'
(Translated from the Russian by Prof. L. Vilas).
" I': M plat o 9, top : righ t), 2.5 - 1 .0 Lm by 2.0 - :I.:J Lm; conid ial cha ins shor t,
1/lII Io(lt:d (lig. 50, C ).
:lllon ics upon CY A (co lo u r pla to 50, B an d b) attain ing a diamc ter 0 1'
I ~ 11 \111 in 14 days a t room temperature (20 -25 OC ), consisting 0 1' a leathery
1II'1'11I:e, su lcate d with nu m erous, radial fu rrow s, and dcprcsse d in the centre;
th ln ruargin su rro u nded b y a frin ge 01' su b mc rged h yphae cxte nd ing 2- 3 mm
buyond th e ae r ial gr ow th ; colo ny su rfaee eovered with a thin mycc1i al
uvcr growth 01' white ae rial, closely interwoven , hyphae giving a su b la n a te
\pl'eal'a nec to th e eolony; med iu m sporing in pale blue-gra y shades (Sg uy, PI.
X X II I, 48 4), with n on -sp orulating a reas remainin g p ersistently white ;
xud.uc in eo lou rless d roplets; odou r laeki n g; ye llow -eoloured pi gmcnt
d fl'nsillg into th e surro u nding aga r ; eolo ny reverse (eolour plat e 50, b ) in
i-Ilow -cream shades; penieilli essentially as deseribed a boye, w ith th e stipcs 0 1'
11\1: con id iop hores mostly arising from th e net work 01' ae rial hyphae.
( :Dlon ies upon MEA (eo lour pla te 50, C a n d e) a ttaini ng a d iametcr of
I ~) 111m in 14 d ays at ro om te m pe rature (20- 25 OC), eonsisting 01' a typi cal1y
dC'('l' ly velvety, loose-t extured b asal felt, zo nate, plane , with th in margin ,
IIITDI!nded by a fringe 01' su b merge d hyphae exte nd ing abou t 2 - 3 mm bey ond
1111' ucrial growth; heavily sporing through out in blue-green shades (Sgu y , PI.
X X XI , 46 1); exudate limited in a mou nt, en meshed in the m yeeli al felt ; odo u r
Inrk iIIg; pale amber p igment d iffu sing into th e surro u n d ing agar; co lony
n -vcrsc (co lou r pla te 50, e) in yello wis h eo lou rs; penieil1i essen tial1y as
I h'sni bed a boye, bu t w ith the sti pes either a r ising from the su b st ratu m a nd
1'1'Il1I1 the ne twork 01' ae r ial hyphae ; co nid ial eh a ins in loosely paral1el colu m na
DI' lan gled .
' I' he speeies deseription is ee ntered u pon cu lt ural stu dies 01' th e typ e strain
1:Il S 11 4.69 = V K M F-I 0n = C M I 140 ,335 = ATC C 22 ,347 = IJ FM 50 14, isolatcd
I'I'DIl\ Syrian d esert soil by V . C h . Ba ghdadi , in 1964.
' I'he correet plaeement 01' the speeies is still ope n to question. It is as signed
1)(:1'(: tentat ively only as a m a tt er 01' eonven ienee .
.11 . Penicillium rapen' Sm ith : fig. 51, colour plate 51, a nd SEM plat e 9 ,
(centre: Ieft ).
Penicil/ium raperi Smith - Trans . Brit . Myeol. Soe. 40: 486-487. 1957. See also
M .M . Kulik, 'A Compilation of Deseriptions of New Penicillium Species' . Agrie.
Handbook No. 351. Agrie. Res. Ser. U.S. Dep. Agrie . p. 70. 1968.
Smith's original diagnosis is as follows:
'On Czapek agar grows restrietedly, 22-25 mm in 9-10 davs, and thereafter
spreading only slightly, matted floeeose about 1 mm thick, at first pale yellow
beeoming mauve in parches. tardily turning pale bluish green all over or almost all
o o o
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O
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over, with drops small, colourless, and reverse dull brown with intense yellow edge,
but with no diffusion of the pigment into the agar, sporing tardily first in the mauve
areas, more freely when the greenish colour appears; on malt agar grows rather
more quickly, 30 mm in 9 days and 42-45 mm in 15 days, pale yellow and whitish
with only traces of pink or mauve and turning greenish only very slowly and
incompletely, with reverse deep dull yellowish brown; on potato-dextrose agar
grows rather more quickly than on Czapek, much as on malt agar, yellow at first
then with mauve centre but with a middle zone of pale bluish green, with reverse at
first brown w ith yellow margin, then with centre purple and margin zoned with
brown and deep greenish yellow; penicilli monoverticillate or. more often, irregular,
with two to three unequal metulae, or with various superimposed verticils
consisting of both metulae and phialides, but with the number of elements at each
stage very small; conidiophores arising from aerial hyphae, very short, mostly less
than 25 Jlm long and 1.3 to 1.5 Jlm in diameter; phialides few in the verticil, usually
divergent, 7-9 by 1.8~2.2 Jlm, narrowed at the base and with fairly long tip; conidia
Sm iths original dcscription 01' the species is given sin ce this ad equatcl y
prcscnts th e spec ies in question whcn first isolat cd . The typc strain OIlS
" 11 1.58 = CMI 71,625 = IF O 8 ,179 = QM 7527 = IJFM 5007 , maintained in a rtificial
cultu re sin ce its isolation from th e soil of a cabbage field near Bedford, in
Septernber 1952, has become predominantIy poorly spor ing , rernaining pcrMistently white upon C A and CYA, not showing any longer th e typical cultura l
rh aracteri sti cs origina lly described by th e author, although still sho wing
cha racteristic det ails of morphology.
O ur not es follow :
Colonies u pon C A (colour plat e 51, A a nd a) a ttaining a di ameter of 35 mm
in 14 days at room temperature (20-2 5 OC ), ca ns isting a l' a close -textured
hnsal felt , velve ty to lanat e; poorly spo ring , remaining per sistentIy white ;
l'olony surface deepl y radiat ely furrowed and depresscd in th e cen tre; evcnly
IlIargin su rro u nde d by a frin ge of submerged h yphac exte nd ing about 1 mm
hcyond th e aerial gro wth ; od our and exudate lacking; pale am ber colou red
I,i).{rnent d iffusin g into th e su rro u nd ing agar ; colony reverse (colou r plat e 51, a)
in sulphur yellow shades; penicilli eithe r strictIy sim ple 01' irregul arly branched
i 11 a divaricat e manner (fig. 5 1, A) , with sti pes ar ising m ostl y as short branch es
I'I'OI!l ae rial hyphae , ranging fro m 25 to 100 .tm in length by 2.3 - 3 .0 uit: in
rlinme te r, with walls smooth, enla rged at th e apex in a vesicle up to 3. 5 .tm in
di.unet er; phialides in div ergent c1uster s of 6- 10 e1ements in th e verticil ,
iu ostl y 8 - 11 .tm by 3.0-3 .5 uui , terminating in conspicuo us, narrowed ne ck;
conidia elliptica l to su bglobose , 2. 5- 3. 3 .tm by 2.5-3. 0 uiu, delicately
roug he ne d (fig. 51, B, a nd SE M piate 9, ce nt re: left) ; con id ial chains loose to
tan gled (fig. 51, C ).
Colonies u pon C YA (colou r plate 51, B and b) attaining a di ameter a l'
;)0 mm in 14 days at roo m temperature (20- 25 OC), sim ila r in texture to th ose
gl'Own upon CA , bu t deep er radiat ely furrowed, with thin , yellow margin
sur rou nded by a fringe of su bme rged hyphae exte nd ing abo ut 1 mm be yond
lile ae rial grow th; stro ngly pulling and crac king th e agar underneath (colour
pla te 51, b); poorl y sporing wi th surface rem aining per sistentI y white with
I race s of pink ; exudate abu ndantIy produced in pink shades ; od our lacking;
hrown colou re d pigm ent d iffu sing into th e surroundin g agar; colony revers e
(colou r piate 51, b) in dark brown-purple colours; peni cilli esse ntially as
dcscribed abo ye , with stipes arising from th e net work 01' ae rial hyphae .
52, Penicillium dimorphosporum Swart: fig. 52, colour plate 52 , and SEM
y ::-O 00
00 0
{:;:~
o e ro:,
~ ,.~;r
.j:.::J
o o iJ
O oS
8
O
0 0
~0.)
(0;0
lll.llJ..LW..I
10
jim
R..
D
t - t
L--....J
100
}-1m
IIlci/lium dimorphosporum Swart - Trans Brlt. Mycol. Soco 55: 310-313. 1970.
S WHI'I 's
01' thc
pl'l':sc:n ls thc spccics in qucs tio n when first isolat ed . The typ e stra in CIIS 456 .70
tloes not show an y longer the typical cultu ra l cha rac teristics o rig ina lly
tlUMl'I'ihecl by th e a utho r , remaining persisten tly wh ite up on a11 media test cd ,
dlhlJlI /{h still sho wing chara ctcristic dctails 01' morphology.
' Colonies upon Czapek's agar slow-growing, reaching a diameter of 2 cm in 14
va. Colonies white at first , turning pale grayish green in the centre with the
lovolopment of the first conidia . Eventually the culture turns a deep reddish brown
xcopt for the white margino Mature colonies are slightly zonate, irregularly
buckled , with agar sometimes being pulled away from the bottom of the culture
lah. Exudate is abundantly produced , pale pink at first but soon turning deep red;
vorse in similar colours with pigment diffusing into the surrounding medium.
onidiophores short, srnooth, unbranched, arising as side branches from trailing
hyph ae at, or slightly aboye, the surface of the mycelial felt, rarely more than 30 11m
lonu by 2 Jlm wide. Phialides variable, in older cultures short, wide and thus
rllvorging . First-formed conidia are oval , srnooth, greenish in culture and measure
- 2.4 Jlm by 1.8-2 Jlm, but conidia produced later, from the moment the culture
turns brown, are globose, roughened by prominent tubercles and bars of pigment,
brownish, and average 3.1 Jlm in diameter with some conidia up to or even over 4
1,m . Some conidia are intermediate in size, shape, and surface, between these two
mnin types.
Colonies on malt extract agar grow slightly faster, produce more greenish conidia
md are slower to change colour . Pigmentation is not as intense and colonies are
browner with very little red at first . Exudate is limited and tinted amber or pale pink .
ccasionally a few patches of yellow encrusted hyphae are produced. The reverse
In yellow brown. Whereas on Czapek's agar the second type of conidium soon
dominares. on rnalt extract agar a considerable number of conidia of the first type
rre found . Intermediate conidia may be numerous as well.'
O u r notes follow :
Colonies upon CA (colour piat e 52 , A a nd a) a tta in ing a di ameter 01' 32 mm
in 14 days a t roo m temperature (20 -25 OC ), co ns isting 01' a close-textu re d
basal felt , irregul arl y folde d a nd radi at ely wrinkled a nd rai sed in th e centre ;
irregularly dissected margin surrounded by a frin ge 01' sub me rge d hyph ac
cx tend ing 3-4 m m beyond th e aerial growth ; heavily spo ring thro ug ho u t,
with colony surface rem aining persistently white ; ex udate lacking; odo u r fain t ,
'mouldy; no coloured pi gm ent diffus ing into th e surrou nding agar; colony
reverse (colour plat e 52, a) in crea m sha des; peni cilli strictly sim ple (fig. 52 ,
A), with sho rt stipes, abou t 25 11m in len gth by 1.8- 2.5 11m in diamet er ,
smoorh-wa llcd, arising Irom aeriul hyph ae: phi nlidcs bottl e-xhap ed , in clus tcrs
o l' 6- 10 elc mc n ts in th c vc r tic il, mostl y 11 lO I~III by :U l - 4,.:{ Jtlll, tcrmin a tcd
in a short-poin te d n cck ; co n id ia 01' two typ cs: The fir st type (Iig. 52, B) are
ellip tical , sm ooth walled , m ostl y 3.0 - 4 .5 Jtrn in lon g axis . The seco nd type are
globose, co nspicuo usly ech in ulate a nd brown colou rcd (lig. 52, e, a nd SEM
piate 9, cen tre : ri gh t), m ostl y 4.5 -5 .0 Jtm in di ametcr ; co nid ial chai ns sho rt ,
loosely parallel (lig . 52, D ).
Colo nies up on CYA (co lour plat e 52, B a nd b) attaini ng a d iameter of
37 mm in 14 d ays at room te rnpe ra ture (20 -25 OC), co nsis ti ng of a tough ,
close- te xtu re d basal felt, partiall y cove re d b y a thi n rnyceli al overgrowth,
giving a powdery appeara nce to th e colo ny ; deepl y rad ia tely wrin kled a nd
sulcate d conce ntr ically in th e cen tre which is irregula rIy folde d and lea th ery ;
poorI y spo ring, with su rface remaining persistently white; ex udate limited in
amo u nt; odo u r lacking ; pal e a mbe r-co lou red pi gm ent diffusing into the
surro u nd ing agar; colon y reverse (co lour plat e 52, b) in yellowi sh cream
shades; pe n icilli essc ntially as desc ribe d a boye.
Colonies upon MEA (colour plat e 52, C a nd c) attain ing a diame te r of
45 mm in 14 days at room tempera ture (20-25 OC ), consis ting of a looser
textured ba sal felt , with a velvety and powd ery a p peara nce, plane in
sub marg inal area and irregul arI y folde d and ra ised in th e ce nt re; thin,
irregul arI y di ssected rnargin , su rrou nde d b y a fringe 01' submerged hyphae
exte nd ing 10 01' more m m beyond th e aeria l growth; heavily spo rin g
throu gh ou t in pale gray shades (Sguy, PI. XXXV, 515); ex udate lacking;
odo ur faint, arom atic; no coloured pi gm ent diffu sin g into th e surrou nd ing
agar; colony reverse (colou r plate 52, c) uncoloured , bu t wi th ce n tre sho wing
th ro ugh green- blac k shades; penicilli esse ntially as described above b u t with
stipes aris ing directl y from sub me rge d h yphae in a radial pat tern ;
The species description is cen te re d u pon th e type strain CBS 456.70 = CMI
149,680 = ATCC 22783 = IJ FM 502 1, isolated fro m a m angrove swa m p soil near
W esternport Bay near M elbourne , V ictoria, Au stralia , by H .I. Swa rt, in
1969.
The co rrect placement 01' th e speeies a ppears to be m ost sa tisfac torily
ass igned to th e P. restnctum se ries on accou nt 01' th e slow growth on most m edi a,
th e sho rt stipes arising from ae rial hyphae , a nd th e presence 01' strongly
echin ula te globose conid ia.
53 . Penicillium striatisporum Stolk : fig. 53, colour plat e 53, and SEM
plate 9 , (bottom : left ).
Penicillium striatisporum Stolk - Antonie van Leeuwenhoek. 35: 268-270. 1969.
Colonies upon CA (co lour plate 53, A and a) grow ing restri ctedl y , attaining
rt
~ ----
rr
0 0
00 O O
O
.
o 0
e
.
G'
O B '? O
O
O 00
@
00
WlllJ..w.J
10
).1m
11'l
L----l
100 um
ER.
;1
lcft): eonid iul chains looscly div crgcnt, up to 60 Jtm lon g (fig. 5:i, C ).
C olonies upon C YA (eolour piule 5:i , B and b) growing u littl e less
restrictedly than aboye, attaining a diamct er 01" :i O mm in 14 days at room
temperature (20-25 OC ), similar in texturc lo thos e grown up on CA, but
showing de ep radial furrows , margin raised and centre depressed, strongly
pulling and cracking the agar underneath (colour pia te 53, b) ; poorly sporing,
with colony surface remaining persistently white; exuda te abundan tly
produced in pale yellow shades; odour lacking; no coloured pigment diffusing
into the surrounding agar; colony reverse (colour plate 53, b) in cinn amon
shades; penicilli essentially as described aboye.
Colonies upon MEA (colour plate 53, C and e) attaining a diameter of
30 mm in 14 days at ro om temperature , consisting of a close-t extured basal
felt, deepl y velvety to floccose in appearance; white , eve n m argin surrounded
by a fringe of submerged hyphae exte ndin g abou t 2 mm beyond th e aerial
growth; colony su rface convex, abou t 1 mm deep in th e cen tre, covered by a
my celial overgrowth of white , slender hyphae , giving a lanat e appearance to
th e colony; heavily sporing through out , formin g conce n tric, differently
coloured zones , ranging from lila c (Sguy, PI. 1, 3) to pastel blue (Sguy, PI.
XXXII , 474) , pa ssin g through orange-yello w sh ad es, dep ending on th e degree
of maturity of con idia; exudate and odou r lacking; pal e yellow-colou red
pigment diffu sin g into th e su rrou nding agar; colony reverse (colour plat e 53, e)
in pal e yellow shade s; penicilli esse n ti ally as described ab oye.
The sp ecies description is cen tered upon th e typ e strai n C BS 705. 68 = CMI
151,749 = ATC C 22,052 = IJFM 5028, isolat ed from leaf litt er of A cacia karroo by
Prof. M .C . Pap endorf, D ep artment of Bota ny, Potchfstroom , Sout h Africa , in
1969.
Penicillium striatisporum Stolk appears mo st satisfac tor ily assigned to th e P.
restrictum series on accoun t of th e restricted growth and th e sho rt stipes arising
as branch es from ae rial hyphae . H owever, it shows a certa in relation ship with
th e P. adametz ii seri es sin ce sorn e strains produce strong ly funiculose colon ies
on M EA.
T he spec ies is probabl y a soil mi crofungus . Prof. M. C. Pap en dorf isolat ed
three addition al strai ns duplicating th e typ e strain in th e structure of th eir
penicilli a nd conidia, from soil taken at a depth of 15 cm. The colonies of th ese
strains on CA are less thi ck, they spo rulate better , in grayish shades, and up on
MEA th ey are thin and stro ngly funi cul ose .
15:J
J~
. 10~
pm
g0I~
...'"
~
l;j
B'
'4
r1~,
~>0 "..:;r
~r0~
'\[jl:;.
"il.
\ ! ~:
\~
q.>
L..--.J
100pm
n~
.<'
~ f.f{.
C olonies upon C A (colour plate 54, A and a) growing restri ctedly , attaining
a d ia meter of 25-30 mm in 14 days at room tempe ratu re (20-25 OC),
uhho ugh sorne strains attain a diameter of 45 mm, consisting of a tough, closel c x t ur ed basal felt , velvety in appearance, radiately wrinkled, with white, eve n
u iurgi n, surrou nded by a fringe of su b merged hyphae exte nding less th an
1 111m beyond the aerial growth; raised in central areas; sporing abundantly in
pode blue -green shades (Sguy , PI. XXX , 49) in su bmarg in al zones , and in
hrown ish gray shades (Sg uy , PI. XXIX, 429); odour a nd exudate lacki n g; no
coloured pigment diffusing into the su rrou nd in g agar; colony reverse (colou r
plate 54, a) in pale cream ti nt s; penici lli strictly simple , wit h short stipcs,
usually borne as sho rt branch es from aerial hyph ae or from th e substratu m,
smooth wa lled , usu ally less th an 50 .tm in length by 1.5-2 .5 .tm in di am etcr;
phialides bot tle shaped, few in the vertici l, usu ally 2-3 eleme nts , m ea su rin g
D.O- 13.0 .tm by 2.5-3.5 uu, term inating in a conspicuously long, narrowed ,
lap er ing neck (fig . 54, A); con id ia globose , stro ng ly echi nula te (fig . 54 , B, and
SEM pla te 9, bottom : right), commonly 4.0 -4.5 .tm in d iameter, sho wing
hrown colou rs in mass.
C olon ies u pon CYA (colour pIate 54 , B a nd b) spre ad ing broadly, attaining
tri !>
'Colonies upon Czapek's agar growlng slow ly, oppeorlng floccose; the penicilli
rru monoverticillate. The colony remains perslstently uncoloured in age at 20-25C.
After 30 days, the colony becomes slightly coloured, in part green, sometimes in
pnrt red-violet . The colony reverse is occasionally partially purple or deep purple.
'ho exudate is uncoloured or yellow, becoming purple in old cultures. The
onidiophores are generally monoverticillate, sometimes branched, 2 tm in
lIometer; phialides measuring 12 tm in length, the conidia are globose, 1.5-2.5 tm
In diameter, smooth walled.
Colonies upon malt extract agar with a wide, white margin, sometimes 5 mm in
w ldth; becoming partially purple-red coloured. It does now grow at 33C.'
(Translated from the Japanese by Masae Yamamoto). (The above description
W llS published without providing a Latin diagnosis).
K ey to spe cies
Paga
1. Colonies with the funiculose habit predominant or well developed.
P. adametzii series . .. . 155
a. Colonies usually developing dull reddish orange to brown shades in
the colony reverse.
1'. Phialides comparatively small, usually less than 10 .tm in length .
aa. Conidia comparatively small, up to 3 .0 .tm in diameter.
1". Conidia smooth walled or nearly so, globose, up to 2.8 .tm in
diameter.
P. kazachstanicum Novobranova . . . . 173
2". Conidia delicately granular, globose to subglobose, ranging
from 2.5 to 3.3 .tm in diameter.
P. adametziiZaleski ... . 157
3". Conidia conspicuously roughened, globose , up to 2.5 .tm in
diameter.
P. albo-cinerascens Chalabuda ... . 175
bb. Conidia comparatively larger, up to 4.0 .tm or more in diamete r.
1". Phialides up to 10 .tm in length, con idia globose , ranging
from 2.5 to 4.0 .tm in diameter , conspicuously roughened.
P. lilacino-echinulatum Abe ex Smith . . . . 170
2". Phialides very small, ranging from 4.0 to 8.0 .tm in length,
with a narrowed, pointed neck; conidia globose, up to 5.0 .tm
in diameter, roughened, with conspicuously long connectives
between them.
P. griseolum Smith .. .. 104
2'. Phialides comparatively large, more than 10 .tm in length.
aa. St ipes of the conidiophores and con idia conspicuously
roughened, especially upon MEA.
1". Phialides atta ining a length of 14 .tm, conidia globose or subglobose , consp icuously spinulose .
P. spinulo-ramigenum Sasaki ex Ramrez .. .. 161
2". Phialides attaining a length of 12 .tm, conidia globose to subglobose , about 3.0-3.5 .tm in diameter, definitely rough .
P. terlikowskiiZaleski . .. . 159
bb. Stipes and conidia smooth-walled or nearly so.
168
166
177
178
180
180
182
Wi th in the series, individual strains vary from essen tially lanate , showin g a
limi ted development of ropes of hyphae, to d efinite1 y funicul ose .
:l:">.
Penicillium adametzii Zale ski: fig. 5 5, colour plate 55 , and SEM platc
Penici/lium niklewski Zaleski - Bull. Acad . Polonaise Sci . Math . Nat. Sr. B, pp.
504-505. 1927.
Colonies upon CA (co lou r pIa te 55, A a n d a) grow ing rather restrictedly,
a lta in in g a diameter of 28 mm in 14 days a t ro om temperature (20-25 OC ),
consistin g of a tough close-textu red basal felt , abou t 1 mm d eep in central
arca, zonate, a ppea r in g lanate , with colony su rface cove re d by a mycelial
B '
lwJ.lJ.wJ
10 )Jm
, >~~/. ~
t j" \)
L---...J
I..--...J
100 )Jm
100 )Jm
overg rowth of tr ailing sim ple hyphae and prominen t funi cles or rop es of
hyphae , eithe r prostrat e , tr ailing, or asce nding ; conid ial areas in pale blu egreen shades (Sg uy, PI. XXVII , 400); ma rgin su rro u nded by a fringe of
submerged hyphae ex ten d ing abou t 1 mm beyond th e aerial grow th ; exudate
lacking to abundantly produced , light am ber coloured; odo ur lacking or
indefinite ; a mbe r-co lou red pigm en t diffu sin g into the surrou nd ing agar;
colony reverse (colour pla te 55, a) in orange shades ; peni cilli str ictly sim ple
(fig . 55, A), rar ely showing on e to two branch es with seco ndary penicilli at
lower levels , with stipes mostly borne as perp endicul ar , short branch es fro m
tr ailing hyphae or rop es of hyphae (fig. 55, D ), com mo nly 20-3 0 /lm but
som etimes up to 50 /lm in len ght by 1.5- 2.0 /lm in diameter , smooth walled;
phialides in small clus ters of 3-8 eleme nts in th e ve rt icil, mostly 5- 7 /lm by
2.0 -2.5 /lm, terminating in a short , narrowed neck ; co nid ia globose to
subg lobose, 2.2-3. 0 /lm in diameter, with walls delica tely rou gh en ed (fig. 55,
B, and SEM plat e 10, top : left); conidial chains tangled , up to 50- 100 /lm in
len gth (fig . 55, C ), or in sho rt well-de fine d colu m ns (fig. 55, D ).
C olo n ies upon C YA (colour pl at e 55,13) nuuining a diamc ter oCiO mm in 14d ays a t room ternperature (20 -25 OC) , duplica ting in te xtu rc a no cu lt ural
cha racter istics th ose gro w n upon C A, hut up lo 2 m m d eep ; conidial arca s in
pale gray-gree n shade s (S guy , PI. XXll , :i:iO); poorIy sporing; exu date ano
odo u r lacking ; amber colou re d pigrnent diffusing in to the su rrou n d in g agar;
colon y rev erse in orange shades (colour plate 55, a); penicilli essentially as
dcscr ib ed a boye .
C olon ies upon MEA (colour pi at e 55, C and c) spread ing less restrictedly,
a tta in ing a diamet er a l' 45 mm in 14 days a t room temperature (20-25 OC),
consisting 01' a thinner felt , deeply velvet y to lana te in appearance , alm ost
plan e , sligh tly rai sed in th e co lony cen tre, cons picuous ly zonate; growing
margin su rrou n de d b y a w ide fringe 01' su b me r ged hyphae exte n di n g a bou t
:3 m m beyond th e ae r ial gro w th; h eavily spor ing in concen tr ic zo nes in very
pale gray-green shades (Sguy , PI. XXII , 330); exu date a nd od our lacking;
pale a m be r pi gment diffusing slowl y into the surrounding ag ar ; col on y reversc
(co lou r pl at e 55 , e) in pal e cream sh ades; penicill i esse n tially as d escribcd
aboye.
The spec ies d escription is centered upon the typ e strain CBS 209. 28 = CMI
:19 ,751 = ATCC 10,407 = N RR L 737= QM 1916=IJFM 500 6 , isola ted frorn soil
under a con ifer forest in Poland , by K. Zal eski.
Strains sh owing cu ltu ral a n d m orphologi cal characte r istics approximatin g
(hose described aboye are cornm only isolated from soil and from m at erial
subj ecte d to soil or dust-borne con ta m ination . Individual stra ins show com rnonly marked differences , with respeet to the degree ofropiness and the rate 01'
growth, without showing su fficie n t bases for separa t ion .
Synonymies
Penicillium jentho-citrinum Biourge - Monographie, La Cellule. 33: 311-313. 1923.
Penicillium paczoskii Zaleski - Bull. Acad. Polonaise Sci. Math . Nat . Sr. B, pp.
505-507. 1927.
":.',;-;'
,.:~:::
:1
." :0 ".",
:.:;::~"
10 ).1m
tS~r
L..--....J
100 jarn
fR.,
somewhat zon ate, plane , with central area usually umbonate; undulating
margin sur rou nde d by a frin ge of subme rge d hyphae extend ing ab ou t 2 mm
beyond th e ae rial growth; medium to he avily sporing throu gh out or in sorne
strains in m arginal a reas only, in pale gr ay-green shades (Sguy , Pi. XXX,
450) , sometimes partially obsc ured by a white m ycelial overgrowth ; odo ur
faint , indefinite ; exudate abundantly produced , as amber coloure d droplet s, or
lackin g; bright yellow coloured pigment diffusin g into th e su rrou nd ing agar ;
colony revers e (colour plat e 56, a) in reddish orange shades; penicilli strictly
sim ple, seldom bran ch ed (fig. 56 , A) , with th e stipes of th e con idiopho res
arising primarily as short branches from aerial hyphae or ropes of hyphae,
usually less th an 100/lm in len gth by 2.0-4.0/lm in diamet er, with wall s
ranging from appare n tly smo oth to conspicuo u sly granula r; phialides in
clusters numbering 5- 10 elements in th e verticil, mostly 9-12/lm by
3. 5 - 4.5 tui , terminating in a con spicuously short , narrowed neck ; con idia
globose to subglobo se, mo stly 2.8 - 4.0 /lm in di ameter , with walls definitely
rou ghen ed (fig. 56, B, a nd SEM plat e 10, top : right) ; conidial chains
lorrn .
e
-:
'.
e
~
10,um
t11
.R..
100
jrrn
1c;:
58. Penicillium griseolum Smith : fig. 58, colour plate 58, and SEM plate
10, (centre: rig ht) .
Penicillium griseolum Smith - Trans. Brit . Mycol. Soco 40: 485. 1957. See
also M.M. Kulik , 'A Compilation of Descriptions of New Penicil/ium Species'.
Agric. Handbook No. 351. Ag ric . Res. Ser. U.S. Dep. Agric. pp. 42-43. 1968.
Smith's original diagnosis follows, since this adequately presents the species in
question:
' Colonies on Czapek's agar spreading moderately quickly but extremely th in,
transparent, showing no visible myce lium but only scattered penicilli , giving a faint
greenish hue to the colony; on malt agar or potato-dextrose agar growing
moderately well, at first pale gray w ith broad white edge, turning darker gray,
matted funiculose about 1 mm thick; reverse brown with a greenish tone (somewhat Iike mustard but rather darker and duller) ; penicilli strictly monoverticillate;
conidiophores arising from aerial hyphae, very short, smooth, 12-35 by 1.2-2.0
.tm ; phialides few in the verticil, abruptly pointed, 6-7 .tm by 1.6-2.0 .tm; ripe
Jf
A~
y=vL
V0
kLl
~~
-==~
'.
.~
L...---...I
100
jrm
--- ~
lu.uJ.w..J
10 prn
R.
e,
conidia globose or nearly so, faintly verrucose, 3.1-4.0 .tm in diameter but mostly in
the range 3.2-3.5 .tm, in parallel chains or forming somewhat loose columns; young
conidia oidium-like, small , cylindrical, swelling only slowly and remaining in chains
attached to the phialides, even on mounting fluid.'
textured basal elt , overl aid by a my cclial ovcrgro wth 01' closcly int crw oven
ae ria l and Iunicul ose hyphac , obs curing almos t tot ally the othe rwise
abu nda n tly produced con id ial structures, rc maini ng persistcntl y whitc,
showing th e gra y-coloure d sporing ar eas especially in m arginal zon cs ;
undulating, thin margin surrou nde d by a frin ge 01' su bme rged hyphac
extend ing abou t 2 m m beyond th e ae rial growth; exuda te and odo u r lacking;
pale bro wn coloured pigment diffu sin g into th e surrounding agar; colony
re ver se (colour plate 58 , b) showing brown colou rs; peni cilli essen tially as
descr ibed aboye.
Colonies upon M EA (colour pIat e 58, C and e) spread ing broadl y , attai n ing
a di ameter 01' 55 mm in 14 days a t room temperature (20-25 OC), cons isting 01'
a den sely textured basal felt , deepl y velvety to lan at e , ove rla id by a m ycelial
network of ae rial hyphae and ro pes of hyphae influen cin g partially th e colony
a ppeara nce; thin m argin su rro unde d by a wid e frin ge of su bme rge d hyphae
exte nd ing abo ut 3- 4 mm beyond th e ae rial grow th; ce ntral a nd su bce n tral
areas heavily spo ring in gra y shades (Sguy, PI. XXI , 310); conid ial structures
are obscured in m a rgin al areas by th e white mycelial ove rgrowth of floccose
ae rial hyphae ; ex udate and odo ur lacking ; no coloured pigm ent diffusin g int o
the su rro u nd ing agar ; colony rever se (colou r pIat e 58 , c) in orange sha de s;
penicilli esse ntially as described ab oy e .
T he species description is ce ntere d up on th e typ e stra in C BS 277 .58 = CMI
71,626 = ATCC 18, 239 = IFO 8.175 = Q M 7,523 = IJFM 5 113, isolated in] uly 1955
by Miss]uliet C . Brown , of the R oyal H ollow ay College, from th e ac id dunes
of Studla nd, Dorset , U K . It is kn own only from th e typ e strain.
i-l
~i4 \~ 1=-~~'-"'
'tr x
l-.-...-J
100 prn
iR.
'o
L '
.c>
59, A) , with stipes sho rt, ari sin g mostly from aeri al hyphae or rop es ofhyphac ,
sometime s from th e subs tra tum, com mo nly 25 p,m or less in len gth , by
2.2-3. 0 p,m in diamet er, with apices enla rge d in a ves icle up to 4 .5 p,m in
diame ter, smoo th walled , septate, phialides in cro wde d clu sters of 8 - 12
cIeme nts in the verticil, bottle sha pe d with a cons picuo us , sho rt, pointed neck ,
mostl y 10-1 2 p,m in len gth by 2.8-4.0 p,m in wid th; conidia broadly elliptica l
to subglob ose, smoo th walled or nearly so (fig. 59, B, and SEM pIate 10,
botto m: left ), mostly 3.0-4.8 p,m by 2.5-4.0 p,m; con idia l chains ta ng led (fig.
59, C) .
Co lonies upon C YA (colour plate 59 , B a nd b) attai ning a diame ter of
50 mm in 14 days at room temper ature (20 -25 OC), duplicating in textu rc
those grown up on CA, but deepl y radiat ely suleate with nume ro us furrows ,
and centre folded in conce ntr ical wrinkles in a cra teri form patt ern ; he avily
spo ring throu gh ou t in blue shades (Sguy, PI. XXX II, 477) , especially in
marg inal areas, a nd partially obsc ure d in central and subce n tral areas by the
white ove rgrowth of aeri al hyphae ; m argin thin , surrou nde d by a narrow
fringe of sub me rge d hyphae ex te ndi ng abo u t 1 m m beyon d th e aerial growth;
colon ics somctimes strongly pulling away thc agnr undcrncath, and crac king
it, thu s permitting the pr escn ce 01' air in the reverso a nd thc production 01'
abundant conidial structures (colour plate 59 , b) ; exudare lacking; odour faint I
indefinite; bright yellow-colou red pigment diffu sing into th e surrounding agar;
colony reverse (colour plate 59 , b) in deep orange shadcs; pcnicilli essentially
as described aboye .
C olonies up on MEA (colour plate 59, C and c) att ain ing a diameter 01'
35 mm in 14 days at room temperature (20 - 25 OC ), consisti ng 01' a closetextured basal felt , ve1vety with sorne centra l funicles , plane , with a thin
m argin surrounded by a wid e fringe ext ending about 7 mm beyond th e aerial
growth ; heavily sporing throughout in blu e shades (Sguy , PI. XXXII , 447);
exudate la cking; odour faint , aromatic ; revers e in ora nge sh ad es (colou r platc
59, c); bright yellow- coloured pigm ent diffusing into th e surrounding agar;
peni cilli esse ntially as described abo ye.
The species de scription is cente red up on th e type strain CBS 3 13.59 = CMI
68 ,227=ATCC 18306=IFo 6 ,055=QM 7, 312=IJFM 5112 , isolat ed from
J apanese soil by S. Ab e in 1955.
UJJJ..l.u.i.JJ
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100 jrrn
McLennan , Ducker and Thrower's ori ginal diagno sis of th e specics follow s
since this adequately presents th e spe cies in question when first isolated:
' Colonies upon malt extract agar spreading broadly, 5-6 cm in 14 days at 24C,
with mycelium forming a dense felt. The upper surface is very funiculose, and the
overall colour is mignonette-green (Ridgway, XXXI), fading towards the edge of the
colony to lemon yellow (Ridgeway, IV), with a tendency to zonation of colour. The
margin of the colony is regular, thin, and white . The reverse of the colony is flat,
pale, cream-coloured (Ridgeway, XVI) with a buff centre.
Colonies on Czapek's agar grow more restrictedly than on rnalt, attaining a
diameter of 2.5 cm in 14 days at 24C. The funiculose character is more pronounced
than on malt; the colonies resemble little nests, the brim formed by a creamy white
lanose growing margin which surrounds the sunken mignonette-green centre. The
reverse is cream to avellaneous in the older areas.
Colonies on Raulin's agar grow more rapidly than on Czapek's agar, 4.5 cm in 14
days at 24C. The nest-like character is more pronounced with a small coloured
sunken centre (Kronberg's green, Ridgway, XXXI) surrounded by a very lanose
white area, 1.5 cm bread , with an abrupt margin oThe reverse is slightly buckled, the
centre antimony yellow, darkening to yellow ochre in age.
Morphology as on malt agar. The colony is characterized by a profuse
development of ropes 10-15 .m wide, consisting of coloured hyphae from which
numerous smooth conidiophores arise, 50-150 .m long. Each conidiophore is
sparsely septated uniformly 2 .m bread , the apex is slightly swollen and bears a few
(3-9) phialides; the spore chains are twisted into short columns. The phialides
which are strongly tapered at their apices are 9-12 by 3 .m. The conidial chains
break up readily, the conidia are smooth and tinted, broadly elliptical, 2-3 by 1.5-2
.m.
This isolate is a strictly monoverticillate form and produces neither ascomata nor
sclerotia, even when newly isolated. The phialides are long and tapering,
resembling those found in the Biverticillata-symmetrica section, also the general
colour scheme is suggestive of this group . However, we have never found other
than monoverticillate conidiophores. The colonies are characterized by the
development of prominent funicles or ropes of hyphae, and hence the form must be
grouped in the P. adametzii series. It differs, however, from the other forms in that
series in the broadly elliptical spores, of the type found in the members of the P.
lividum series.'
L::::=-----
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~~
changing to Tea Green; exud ate abundant , colourloss; roverse in near Iight
vinaceous lilac in 6-7 days, changing to yellow shades, and th e surrounding agar In
pale yellow shades; penicilli strictly monoverticillate; conidiophores short, up to
20-40 .tm in length by 1.2-1.9 .tm in diameter, with walls smooth or nearly so, and
apices enlarged up to 3.0-6.2 .tm in diameter; phialides in crowded c1usters,
numbering 6-15 in the verticil, mostly 5.0-7.0 .tm or rarely 9.3 .tm in length by
1.5-2.5 .tm in diameter, acute type with smooth walls or nearly so; conidia globose
to subglobose, w ith typically echinulate or verruculose walls, mostly 2.1-2.8 .tm in
diameter; chains of conidia in parallel or loase columns, commonly up to 30-60 .tm
in length; and conidia showing conspicuously echinulate or verruculose walls in
electro n microscopv .'
00
0 0 0 O
00 0 0 0
BOO
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l.WJ1J.=J
10 jrrn
,~
by H network (JI' ropes and trailing hyphac, disponed in a zonu te pattcrn, wit h
1II1I I'!{ina l a rcas plano an d ce n tra l zo nes decp ly rud iatcly wrinkl cd ; thin,
1"1'l:glllar1y dissccted ma rgin su rroundcd b y a widc fringc 01' su b rne rgcd hyph ac
exic nd ing abou t :~ mm beyon d the aerial grow th; colony su rface appear ing
rucmbrunous ; sparse ly spo ring; exudate and odo u r lacki n g ; no coloured
pigrm:nt d iffu sing into th e su r ro u ndi ng agar ; colo ny rcv c rse (colour pia te 62 , e)
in cin na rno n sh ad cs ; penicilli esse n tially as d cscribed aboye , bu t more
rbu ndant ly produced , a r ising m ostly frorn trailing hyphae and from rop es 01'
hyph ae (lig. 62 , C ).
The species diagn osis is ce n te re d upon th e type st rain AT C C 24 ,722 = C IlS
1!). 74 = VKM F-1750 = C M I 174 ,720 = IJ FM 7112 , isolated from Pirus malus L.
xu rlacc in th e Alma-Ata region, Sov iet Soc ia list R epublic a l' K azach stan ,
USS R, by T. I. N ov ob ranova, July , 1967 . It is actually kn own on ly frorn the
I ype strain.
The co rrect placement al' this species a p pears to be m ost sa tisfac to r ily
lIssigna ble with in th e P. adametzii se ries on accou n t of its str ictly sim ple
pcnicilli , a nd the strong fu n icu lose characte r a l' th e colo n ies .
o
0 0
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.
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100 jim
.;''>--,
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nemat ophagou s fu ng i, usu ally less th an 50 p,m in len gth by 1.8 - 2.5 p,m in
diamet er, wi th apices enlarged in a vesicle up to 3. 0 usi: in d ia me te r, smooth
walled ; phialides com paratively small, bottle shaped, in clus te rs of 3- 10
eleme nts in th e verticil, com monly 6-9 p,m by 2. 3- 3.0 p,m , ab ru ptly tap er ed
in a short , n arrow neck ; co nidia globose, u su ally 2.5 utt: in di amet er ,
delicatel y sp in ulose (Fig. 63, B, and SEM plate 11, cent re: left); con id ial
chains loosely parallel to tangled (fig. 63, C ) .
Colonies upon CYA (colour plat e 63, B a nd b ) duplicating in texture those
grown up on C A, but spreadi ng mor e rapi dly , attaining a diameter of 35 mm
in 14 days at room temperature (20 - 25 OC ), stro ng ly and deepl y ra diately
wri nkled, with conce nt rical ridge s in the colony centre, whi ch is also
irregul arl y fold ed in a crateriform patte rn, with surface cracked; better sporing
in pale blue-gre en shade s (Sg uy, PI. XXX , 44 5), espec ially in marginal a reas ;
eve n margin su rrou nde d by a narrow frin ge of submerged hyphae exte nd ing
less than 1 mm beyond th e ae rial growth; odo u r lacking; exudate as small
~Y)
~@@
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~
10pm
R.
t1 ropl ct s e n mc shed in thc my ccli al lcl t; pu le nmbcr pigm c nt dilfu sin g into the
urro u nd ing agar ; colon y rev ers o (co lou r platc 6:1, b) in orange lo cin namo n
ha rlc; pcnicill i csse ntially as dcscribcd a b ovc ,
C olo n ies upon M EA (col our plato 6:i , C a n d e) grow ing m ore rapidly,
ru uini ng a c1iamet er 01' 50 mm in 14 day s al room tcmperaturc (20 -25 OC ),
etlllsisting of dense basal fel t ovc rla id by a close ly wov en network ofla n atc ami
lun icul osc hyphae lending a lanate appearance to the colony surfacc, planc,
wh h I ult s of white aerial hyphac in the ce n tre; thin evenly margin su r ro u ndc d
by a wid e fringe of subrncrged hyphae ext ending about 5 mm beyond th e acr ia l
ro wt h; heavily sporing throughout in blue-gray shades (Sguy , PI. XXX ,
111:1 , 188) ; exudate and odour la cking; no coloured pigment diffusing into the
au rro u ncl in g a ga r ; colony reverse (colour pIate 63 , c) in brown shades; pen icilli
cssc n tia lly a s des cribed aboye, but showing more divaricate penicilli.
'I'he species description is ce n te red upon the type strain CBS 219.66 = AT C C
11I ,:ln=vKM F-1037=IJFM 7051 , isolated frorn uncultivated soil in the
cuviro ns of Ki ev, USSR, b y T .V . Chalabuda in 1949 . It is at p rescnt kn ow n
o ldy rom the type strain .
' I'he co r rect placement of the species appears to be m ost sat isfactor ily
lssig-n able within th e P. adametzii series on account of its pred ominant simple
Iu-uicilli and the strong funiculose charac te r of the colonies , but the possibllitlca
I hal P. albo-cinerascens Chal abuda m ay represent a sp ecies tra ns itio nal het wcc n
11u: Monoverticillat a and th e Asymmetrica-divaricata Penicilli a, sUPPOI'lt:d
m.rinly by th e presence of divaricat e penicilli upon sorne su b st rat a ( M EA),
shou ld not be overlook ed .
l'cnicillium sacculum Sub-series , su b -ser ies nov .
Hyphae sterilia reptabundis, crassae, hyalinae, septatae; cum stipitibus brevis,
irregularit er ramosis, ramis ultimis phialidis ferentibus; phialidae dense compressae ,
brevia, plusminusve ovoideae; conidia singulares vel in catenis duorum, triorum;
asperula.
Species typicalis : Penicillium sacculum Dale.
rcrn ain cd u nidcntified since 1914, one 01' the: num es bc ing P. .sacculum. T ho m
( 1930), alt er cxa mi n ing it , regarded Mi ss Da lc's spccies as Scopulariopsis repens
Bain ier. R aper and Thom (1949) assign ed P. .sacculum to Scopulariopsis on th e
b asis 01' T homs earl ier findings , a nd without cxa m in ing a nd stu dy ing any
fresh m at erial. This species wa s list ed by C hes te r a nd Thornton (1956) as
Scopulanopsis sacculum (Dale).
H u gh es ( 1953) h as sho wn th a t Scopulanopsis is n ot rel at ed a t all to Penicillium,
since th e two ge ne ra have entirely d iffere n t m ethod s 01' spo re formation . I n
Penicillium th e spores a re phiall ospores, while in Scopulariopsis th ey are
annellospores .
A ca re fu l exam inatio n 01' P. sacculum has fa iled to det ect a n nelatio n in th e
sporogenou s cells, wh ich on th e othe r hand show all th e characte ristics 01' true
phiali d es.
In 1961 G . Smith create d th e new genus Eladia for th is fun gu s, on the b asis
01' th e fact th at th e spo re- pro ducing cells wer e phialides.
In our op in io n thi s spec ies m a y be cons ide re d as a ve ry primitive form of
Penicillium, co ns isting 01' poorl y d iffere n tia ted sti pes, septate, m ore or less
a rticu la te, b earing monoverticillat e clu sters 01' phial id es, producing
consp icuously stron gly ech in ula te co n id ia.
The species shows a cer ta in relati on shi p with th e P. adametzii series on
account 01' the funicul ose characte r 01' th e co lon ies, espec ia lly when cu ltiva ted
on M EA .
Synonymies
Scopulariopsis sacculum (Dale) Chester et Thornton - Trans. Brit. Mycol. Soc o
39: 301-313. 1956.
Eladia saccula Smith - Trans . Brit. Mycol. Soco 44: 42-50. 1961.
Colon ies upon CA (co lour plat e 64, A a nd a) gro wing ve ry restrictedl y , mostly
sub me rge d, a ttain ing a di ameter 01' 15 mm in 14 da ys a t room tem pera ture
(20- 25 OC ), co nsist ing 01' a loose-textured basal felt , ve lve ty , whi te, somewhat
za nate, with th in margin largely su bmerged (4- 5 mm wide) ; sparin gly
spo ring, with co n id ial fru ctification s a r ising from loosely in terwov en ae r ial
--
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~
10
jrrn
eR.
a di amctcr 0 1' 5:)-60 IlIIl1 in 14 day s al room remp crnturc (20-25 OC),
cons isting 01' a loosc-t cxturcd basal clt , vclv et y in a ppcu ru ncc, co nsp icuously
za na te, showing a thin ove rgro wth 01' sno w whi te, looscly int crwoven acrial
hyphae a nd rop es 01' hyphae from whi ch arise the abu ndan tly produced
con id ial structures, rem aining per sistently white; th e colony surfa ce plan c ,
with gro wing m argin surrounded by a wid e frin ge 01' su bmerged hypha
ex tend ing up to 5 mm beyond th e ae rial gro wth ; odour and ex udate lackin g;
no colou re d pigment di ffu sin g into th e surrounding agar; penicilli abu nda nt ly
produced throughout , esse ntially as described aboye; colony reverse (colour
plate 64 , c) in ora nge shades.
The species diagnosis is ce ntere d on strain c as 230 .6 1 (white variant),
isolated by W . Gams from Austrian soil. The othe r strains 01' P. sacculum Dale
kept in th e Internati onal Fungus Collect ions duplicate this strain in cult ural
and morphological characte ristics with th e exception 01' th e green colou r sho wn
by th ese forms in stead 01' th e persistently white colou r 01' strain css 230 .61 .
Smith's ori ginal di agnosis of Eladia saccula follows, sin ce thi s ade q uately
presented P. sacculum D ale:
' On malt agar growing moderately well, dull gray-green with white edge during
the growing period, gradually becoming brownish green, velvety, slightly wrinkled;
reverse grayish; colonies on potato agar growing somewhat more slowly, otherwise
similar; colonies on Czapek's agar thin and sparse, growing very slowly; on cornmeal agar growing slightly better than on Czapek's agar, with colonies definitely
arachnoid; on malt agar with 40% sucrose growing slowly but colon ies eventually
typical, gray-green, smooth velvety; superficial hyphae funiculose, hyaline, coarse,
septate, more 01' less artic late. 3.5 to 6 /lm in diameter.'
~~~
;1 \
\+j C ~
"'" ,! ",;3
,
~
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100 JJm
'\
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with apices vcry slightly c n la rgcd ; phialldcs usunlly COJlsisling 01' a cluster 01'
2-5 ele rnc n ts in th e verti cil , 6.0 -7 .51t1ll by 1. :> -2.0 Itlll, tcrminat in g in a
conspicuous tape red neck , so rncwha t div crgent ; con id ia globoso to su bglob ose
when mature, with ends apiculate when young, 2 .0-2 .5 tun, occasionally up
to 3 tui: in diamet er , with walls appearing slightly irregu lar 01' in sorne strains
definitely roughened (Fig. 65, B, and SEM plate 11, bottom: left); co n id ial
chains very short a nd loosely divergent (fig . 65, C).
C olonies upon CYA (colour pi ate 65 , B and b) attaining a diamet er 01' about
20 - 25 mm in 14 da ys at room temperature (20- 25 OC ), con sisting 01' a clos e
textured and tough basal felt, duplicating in texture those gro wn upon CA, but
abruptly raised at the colon y centre in a crateriform pattern; exu da te very
abundantly produced , either as large topaz yellow drops 01' minute droplets;
colon y surface sulcated b y numerous radi al furrows, remaining persistently
white , and slightly sp oring, with undulating margin surrounded by a very
narrow fringe not exceed in g 1 mm beyond th e aerial growth ; arnber-co loured
pigment diffusing into th e surround in g aga r ; colony reverse (colour piat e 65,
b) in deep vin aceous shades; penicilli essentiall y as described aboye.
Colonies upon MEA (colour plate 65, C and e) attaining a diamet er 01' abou t
35 mm in 14 days at room temperature (20 -25 OC ), consisting 01' a looser
textured basal felt, with a wide transparent m argin , su rro u nd ed by a frin ge 01'
su b me r ged h yphae extend in g more than 10 mm be yond th e aerial growth ; no t
furrowed , plane , more 01' less floccose, with ce n tre slightly raised a nd fold ed ;
more 0 1' less zonate ; h eavily sporing in cen tral a nd subcentral zone s in
artemisia green shades (Sguy, PI. XXVII, 404) ; exudate and odour lacking;
colony rev er se (colour plat e 65 , c) in brown sh ades with a green cast show in g
through, rather than vinaceous ; penicilli as aboye .
The spe cies d escription is cen te re d upon strain C BS 514.7 3 = NRRL
2,063 = IJFM 7262 , isolated in 1945 by Prof. G .W . Martin frorn th e sidewall 01'
a tent in th e area 01' Hollandia, N ew Guinea, which represents an extre m e type
by producing conidia that a re consp icu ously ech in ula te. In colony ch a rac te ristics , this cu ltu re duplicates Gi lm an and Abbott 's type, originally isolated
frorn Utah soil, in 1927, CES 389 .48 = NRRL 739 = ATCC 10 ,514 = IJFM 5123 .
66 . Penicillium phoeniceum van Beyma: fig . 66, colour plate 66, and SEM
00
. 0 0 00
00 o O
O
000
00
00 O
B 00 0 O
O O 00 0
00 O
lLw.J..wJJ
\,
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10 prn
'<ji
1
\U
R .
100 prn
with ce n tre rai scd and irrcgularly Ioklcd in a cra tcrifor m patt crn ; cven margin
surrounded by a fringe 01' su b rne rgc d hyph ae extc nd ing about 1 mm beyond
th e ae rial growth; heavily sporing es pec ially in m arginal arc as in pale bluegreen sh ades (S guy, PI. XXX , 443) , with th e remaining zones obs cured by
th e white ove rg ro wth 01' close ly interwoven ae rial hyphae ; odour faint,
indefinite ; ex udate lacking; a m be r-c olou re d pi grnent diffusing int o th e
su rro u nd ing agar; colony reverse (colour plate 66 , b) in pal e brown to
cin namo n shades , with th e colo ny stron gly pulling away a nd crac king th e agar ,
permitting ae ro bic cond itions underneath a nd allowing a b u nd an t sporu la tion
in th e colo ny reverse (colour plat e 66, b) ; penicilli esse n tially as described
aboye .
C olonies upon MEA (colour plat e 66 , C and e) attain ing a di ameter of
40 mm in 14 d ays at ro om temperature (20 - 25 OC ), cons isting of a less d ens e
basal felt , plane in marginal areas and fold ed and raised in th e cen tre, with
thin m argin surro u nde d b y a wid e fringe 01' submer ged hyphae exte nd ing
abou t 6 mm beyond th e aerial growth; vel vet y; hea vily spo ring in ar te m isia
gre en shades (Sguy , PI. XXX, 438); odo u r and exudate lacking; purple-redcolou re d pi gment diffu sing into the su rrou nd in g aga r; co lony reverse (colour
plat e 66, c) in deep purple-red sh ad es; penicilli esse n tially as described aboye ,
but with th e stipes 01' th e con id iop ho res m ostly arising from th e su bstra tu m and
from tr ailing ae rial hyph ae .
The spec ies description is ce n te red up on th e type strain CBS 249 .32 = CMI
40 ,85 8= ATCC 10,481=NRRL 2,070= QM 7,608=BKM F- 321=IJFM 5 122 ,
isolat ed from soot y m ould of a palm (Ph oenix sp. ) at Baarn , H olland , by F .H .
va n Beyma, in 1933.
her e as a species 01' dubiou s po sition since th e au tho r 01' th e presen t study was
not able to exam ine and study th e typ e stra in 01' th e sp ecies in question ,
because all attempts to ob ta in it from Internation al Fungus Collecti ons failed.
Therefore , it has not been included in th e key to sp ecies.
Sa saki ' s original description follows :
'Colonies upon Czapek's solution agar growing restrietedly, with eenter 1.5-3
mm deep, wrinkled, funieulose, margin white, about 1 mm wide, eonidial areas tea
Ill rJ
Colon ies gr owing usuall y ra ther restrict edl y , cons isting of a close-textured
basal felt , wi th su rface a p pearing velve ty or n early so ; fre quen tly p oorly
sporing during th e first 8-10 d ays ; sometimes spread ing broadly , tending to
be lanat e , rangin g from slig htly to heavily sporing .
The stipes of th e co n id ioph ores eithe r prostrate or ascend ing , arising
primar ily from th e su bs tratu m or from creep ing ae ria l hyphae , typi call y
ra m ified, w ith th e ramifications eithe r one celled (metula-like) or lon g a nd
sep ta te, co m monly unequ al in length , sim ple or rebranch ed , not producing
de fin ite a p ical ve rticils or m etulae .
Penicil1i typi cally simple , b orne te r mi nally on th e a pe x of the ramified
stipe s, but keeping always its monoverticillat e characte r , altho ugh rep eat ed
d iva ricate ram ifica tion at var ious levels p recludes a definite or ganizat ion .
C onidia co m para tively small, ranging from globose to stro ngly ellip tical ,
a nd frorn smooth to definitely ro u ghe ne d, dep ending upon th e spec ies .
K ey to species
Pag
B. Stipes of the conidiophores mostly ramified , occasionally twice, each
branch bearing a terminal and simple penicillus but not arranged as a
definite apical verticil of metulae .
RAMIGENA Series . . . . 186
1. Colonies growing restrictedly upon all substrata , mostly 15-25 mm in
diameter in 14 days.
a. Conidia defin itely elliptical, smooth-walled .
1'. Colony surface spor ing in gray-green shades; conidia strongly
elliptical to narrowly cylindrical , with rounded ends, not apicu late.
P. capsulatum Raper et Fennell .... 187
2'. Colony surface spor ing in blue-green shades; conidia elliptical
wi th ends somewhat pointed .
P. cyaneum (Bainier et Sartory) Biourge .... 189
b. Conidia globose, ovate , or slightly elliptical, w ith walls roughened .
1' . Conidia globose or nearly so; conidial chains divergent, not
form ing well-defined columns.
P. waksmaniiZa leski .. .. 191
2'. Conidia ovate to slightly elliptical; conidial chains parallel,
forming well -defined compact columns.
P. charlesii Smith .. . . 193
2. Colonies growing more rapidly upon most substrata , usually 45-50
mm in diameter in 14 days.
a. Conidia roughened , echinula te; colony reverse in vinaceous to
reddish to reddish brown shades.
P. velutinum van Beyma . . . . 195
b. Conidia smooth walled; colonies producing brigh t yellow pigment
in the reverse, diffusing into the surrounding agar. Velvety .
P. citrinum series . .. . 351
Isee The Asymmetrica-velutinasubsection)
c. Conidia smooth wa lled; colony reverse in orange shades ; deeply
floccose.
P. sartoryiThom . .. . 197
67. Penicillium capsulatum Raper et Fcnncll: fig. 67, colou r platc 67, and
SEM plat c 12, (top : lcft).
lw.J..l=1J
10
.:
jrrn
fR. ,
-,
~.~
~
100
jrrn
Penicillium capsulatum Raper et Fennell - Mycologia . 40: 528-530. 1948. See also
Raper and Thorn, 'A Manual of the Penicillia', pp . 242-244. 1949.
\IV
(colour pia te 67, a) un colou red 01' in palo yellow-grec n sha dcs ; peni cill i
typi call y sim ple , borne on stipes 01' va ryin g lcn gth (Ii g. 67, A a nd C ), and
occas ionally formin g clu ste rs, but cons iste n tly ret ainin g th cir mon overticilla tc
character; th e stipes a l' th e ca n idiaphores arise prim aril y frorn cree ping 01'
interl acin g aerial hyph ae (fig. 67, C), rangin g frorn very sho rt up to 100 p.m in
len gth by 2.0- 3.0 p.m in di am eter, smooth walled ; phialides in whorls,
rangin g from 4- 5 up to 8- 10 ele me nts in th e verti cil, u su al1y paral1el, mostly
8-11 p.m by 2- 3 p.m , terminatin g in a short , tap er ed neck; con id ia stron gly
el1ipt ical, with ro unde d ends, mostly 3.0 - 7.0 p.m by 2.0 - 2.8 p.m in diameter ,
smooth wa lled 0 1' nearl y so (SEM pIate 12, top : left) ; conid ial chains sho wing
cons picuo us ly lon g ca nnec tives, and adhe ring in lon g, well-defined colu m ns
(fig. 67, C ), a nd (fig. 67, B) .
Colonies up on C YA (colour pIate 67, B and b) atta ining a diameter 01'
35 mm in 14 days at room temperature (20-25 OC) , duplicating in texture
those grown up on C A, but with the colony surface deepl y wrinkl ed in a radial
pattern , a nd with the centre depressed in a crateriform way , a nd cons picuo usly
crac ked, zonate; medium sparing in pale blue-green sh ad es (Sguy , PI.
XXXI , 463); eve n m ar gin su rro u nde d by a wide frin ge 01' subme rge d hyphae
exte nd ing abou t 2 mm b eyond the aer ial growth; exudate a nd odo u r lackin g ;
pale ambe r -coloured pigm en t diffusin g into the surrou nd ing agar; colony
reverse (colour pIate 67, b) in yellow-cream shades; penicil1i esse ntially as
described aboye, but with stipes arising m ostly fro m trailing ae rial hyphae and
from ropes 01' hyphae.
Colonies upon M EA (colour plat e 67, C and c) attaining a diameter 01'
35 m m in 14 days at roo m te m pera tu re (20 - 25 OC ) , cons isting 0 1' a loaser
text ured felt , appearing deepl y velve ty, not furrowed , abo u t 2 mm deep in the
centre; heav ily spo ring throu ghout in deep blue-gr een shade s (Sguy , PI.
X XX , 446); gra wing margin wh ite , surrou nde d by a wide fringe extend ing
abo u t 2-3 mm beyond th e aeri al grow th; exudate and odo ur lacking ; ca loured
pigment not diffusin g into the surrou nd ing agar; colony reverse (colour pIa te
67, c) uncoloured ; peni cilli esse ntially as described aboye, but with th e stipes
u suall y longer , up to 200 p.m in len gth , ar ising primarily from th e substratum ,
fro m tr ailing hyphae 0 1' from rope s 0 1' hyphae .
T he spec ies description is cente red upon th e typ e stra in C BS 301.48 = C M I
40,576 = ATCC 10,402 = NR R L 2,056 = Q M 4,869 = IJFM 5120, isolated in th e
P an ama Canal Zon e fra m an optical instru ment by DI'. W. G . Hu tchinson in
1945.
Add itional strains representing this spec ies have been rep eat edl y recovered
from deteriora tin g military eq ui pme nt in tropical and sub-tropi cal countries,
thu s suggesting its probable wid e distribu tion in such areas.
11
R.,
~'
1" e 1V'
~\;~::&>~
j)l-.-.....J
100
jrrn
Penicillium cyaneum (Bainier et Sartory) Biourge - Monographie, Liste onomastique, La Cellule. 33: 102. 1933. Emend. Ch. Thom, 'The Penicillia', pp. 226-228.
1930. See also Raper and Thom, 'A Manual of the Penicillia', pp. 244-246. 1949.
Synonymy
Citromyces cyaneus Bainier et Sartory - Bull. Trim. Soco Mycol. France. 29:
157-161 .1913.
Citromyces musae Bainier et Sartory - Bull. Trim. Soco Mycol. France. 29:
154-157. 1913.
1- 2 mm dcep , dccply velvet y lo lan at e in appcnrn ncc from an ovc rg rowth 01'
white aerial sterile hyph ae inllucu cin g grca tly th e colony ap pcura ncc; azo na le;
m argin entircly surro u nde d by a very narrow fringc 01' su bme rged hyph ae
exte nd ing less th an 1 mm beyond th e ae rial gro wth; poorl y sporing in palc
gray-green shades (Sguy, PI. XXIX , 429) but with th e colony surface
remaining persistently white th rou gh out owing to th e m yceli al ove rg rowth;
exudate a nd odo ur lacking; coloured pigm ent not dil'fusin g into thc
su rro u nd ing agar; colony reverse (colou r plat e 68, a ) colou rless 01' becoming
yellowish in age ; penicilli sim ple (fig. 68, A) , with th e stipes a ris ing from the
su bs tra tu m 0 1' as branch es fro m ascending ae rial hyphae , smooth walled , up to
100- 200/lm in length by 2.0 -2 .8 /lm in d ia mete r , irregul arly bra nc he d,
occasionally bearing apica l gro u ps 0 1' penicilli ; phialides comparatively large ,
mo stly 13-16/lm by 3.0-4.0 iuu , terminating in a cons picuo us ly tapering
narrowed neck , u sually in clusters 01' 3- 10 ele me nts in th e ve rt icil; conid ia
elliptica l with mor e 0 1' less point ed ends , sm oo th walled 01' nearly so (fig. 68, B,
and SE M plat e 12, top: ri ght) , mostly 2.5 -5 .0 p.m by 2 .0-3 .0 p.m ; conid ial
cha ins loosely parallel 01' ta ng led (fig . 68 , C) .
Colonies upon C YA (colour plat e 68, B and b) attaining a d iam et er a l'
37 mm in 14 days at room te m pe ra tu re, cons isting 0 1' a tou gh , close- tex tu red
basal felt , deep velvet y in ap peara nce, stro ngly and deeply sulcated with
numerou s radial fur row s, rai sed in th e centre in a crate riform pattern , ove rlai d
by a thin m ycelial ove rg ro wth 0 1' closel y interwoven ae rial hyphae , infl uen cing
th e colony appearance; margin entire su rro u nde d by a very narro w fringe of
su bme rged hyphae , exte nd ing less th an 1 mm beyond the ae rial gro wth ;
spo ring abu nda ntly in deep gree n shades (Sguy, PI. XXVIII , 4 16) in
m argin al areas, with non-sporulating a reas remaining persistently colou re d in
cream shades owing to th e m ycelial ove rg ro wth; exudate a nd odo u r lacking;
am be r-co lou red pi gm ent diffusin g into th e su rro u nd ing agar; colony reverse in
yellowish sha des (colour plat e 68, b); penicilli essentially as described aboye.
Colon ies upon M EA (colour pIate 68, C and e) attain ing a d iamet er 01'
40 mm in 14 days at roo m temperature (20 -25 OC), looser tex tu re d than the
preceding , deepl y velvety to la nat e , somewhat deepe r in th e ce ntre, up to
3 mm thick , overla id by a m ycelial overgrowth 0 1' loosel y interwoven floccose
ae rial hyphae influencin g the colony ap peara nce; thin , eve n m argin
surrou nde d by a fringe 0 1' submerged hyphae exte nd ing abo ut 2 mm beyond
th e ae rial growth; heavily spo ring in th e colony cent re in blue-gr een shades
(Sguy, PI. XXX , 443) ; exu date lacking; odo u r faint , aro matic; pale am be rcoloured pi gm ent d iffusin g into th e su rround ing agar; colony rever se (colour
pIate 68, c) in yellow ish sh ades; peni cilli esse ntially as described abo ye , but
with th e stipes eve n longer th an on C A, sho wi ng fewer phialides in th e ve rticil.
T he species description is cente re d upon th e typ e strain CBS 3 15.48 = CM I
39, 744 =ATCC 10,432= NRRL 775=IFO 5,33 7 =QM 7,516=IJ FM 3759, isolat ed
from soil by Ba inier in 1913 .
/l ll el
llH
!\,t i-t
J;~V
~~~. .
::=1?
(" 1'..'
':fu1
: ..
.'t- . o
Penicillium waksmani Zaleski - Bu!. Acad. Polonaise Sci. Math . Nat. Sr. B, pp .
468-469. 1927. See also Ch. Thom, 'The Penicillia', pp. 230-231; also Rapar and
Thom, ' A Manual of the Penicillia' , pp. 246-248. 1949.
Probable synonymies
Citromyces affinis Bainier et Sartory - Bul!. Trim . SOC oMyco!. France. 28: 39-43.
1913.
Citromyces brevis Bainier et Sartory - Bull , Trim . Soco Myco!. France. 28: 43-45.
1912.
Citromyces minutus Bainier et Sartory - Bull. Trim . Soc oMyco!. France. 29: 137144. 1913.
Citromyces ramosus Bainier et Sartory - Bul!. Trim. Soco Myco!. France. 29:
144-148. 1913.
C olon ies upon C A (colour plut e (;9, A and a) growing mod cratcly , auainin g
a diameter 01' 40 1II111 in 14 days at room tcmperaturc (20 -25 OC), co nsisting 01'
a close-textured basal ICIt, ovcrlaid by a clos cly wovcn nctwork 01' deli cat e
hyphae, giving a deep vclvety appearance to the colony suracc; thin margin
surrounded by a fringe of submerged hyphae extending about 2 mm beyond
the aerial growth; colony surface deeply furrowed in a radial pattern, raised in
the centre, appearing crateriform ; heavily sporing throughout in deep bluegreen shades (Sguy , PI. XXX, 447), obscured in sectors by the thin
overgrowth 01' white sterile aerial hyphae; exudate abundantly produced, as
large yellow drops; odour lacking; pale amber-coloured pigment diffusing into
the surrounding agar; colony reverse (colour piate 69, a) in bright yelloworange shades; penicilli monoverticillate , 01't en appearing singly, sometimes in
irregular clusters 01' 2-3 01' more elements, borne on separate branches and
retaining their individual monoverticillate character (fig. 69, A and C), with
the stipes arising from the basal felt as ascending branches, ranging from very
short to 100-200 tm in length by 1.5-3.0 tm in diameter, with apices
somewhat enlarged in a vesicle up to 3.0 tm in diameter; phialides
comparatively small, in compact clusters 01' 6-10 elements in the verticil,
mostly 7-11 tm by 2.5-4.0 uit , terminating in a short, narrowed neck;
conidia globose to subglobose, mostly 2.5-3 .0 tm in diameter, delicately
roughened (fig . 69 , B, and SEM plate 12, centre : left); conidial chains
divergent, becoming tanglcd, 50-100 tm in length (fig . 69, C) .
Colonies upon CYA (colour piate 69, B and b) attaining a diameter 01'
55-60 mm in 14 days at room temperature, duplicating in texture those grown
upon CA, but strongly and irregularly furrowed, raised in the centre in a
crateriform pattern, and cracking; heavily sporing in the same blue-green
shades (Sguy, PI. XXX, 447) as the preceding, especially in marginal areas,
but conidial areas obscured in central areas by the thin overgrowth 01' white,
closely woven aerial hyphae overlaying the colony surface; thin, even margin
surrounded by a wide fringe 01' submerged hyphae, extending about 3-4 mm
beyond the aerial growth; odour faint, aromatic; exudate abundantly produced
as large, deep amber drops ; amber-coloured pigment diffusing into the
surrounding agar; colony reverse (colour plate 69, b) in orange to cinnamon
shades; penicilli essentially as described aboye .
Colonies upon MEA (colour plate 69, C and c) attaining a diameter 01'
50 mm in 14 days at room temperature (20- 25 OC), consisting 01' a looser
textured basal felt, conspicuously zonate, overlaid in sectors by a thin mycelial
overgrowth 01' white floccose aerial hyphae; colony surface plane, slightly
furrowed in the centre; thin, even margin surrounded by a wide fringe 01' submerged hyphae extending 10 mm 01' more beyond the aerial growth ; heavily
sporing throughout in gray-green shades (Sguy, PI. XXIX, 429); exudate
and odour lacking; coloured pigment not diffusing into the surrounding agar;
I ~ ;l
oony rev crse (colour plat e 69 , e) ;n pu le yellow sha dcs : pcn ici11 cssc n t ially a
:)cscribed abovc but more co n unon ly show ing' ramifi cati on s in a terminal
c rticil and co n id ial cha ins lon gc r , up lo 100 ~1Il 01' m ore , tan glcd.
Spcc ics d escription is cen tc red upon the type stra in C BS 230 .2B = C M I
:19 ,746 = AT C C 10,516 = NRRL 777 = 11'0 7, 7'3 7 = QM 7,681 = IJFM :1757, isolat cd
rom forest so il in Pol and by K . Zal eski , in 1927 .
Penicillium w aksmanii a ppe a rs to be clos ely related to P. corylophilum Dierckx , both
in its basic cu ltu ral ap peara nce a nd in th e ge neral m orphological charactc rs ol'
its penicilli. H owe ver , in th e former species the penicilli a re mure regul arly
mon overticill a te , a nd th e co nid ia a re globose and co ns p icuous ly rou gh en ed ,
wh ile th e la tt er species shows smooth -walle d, elliptica l to su bglobose co nidia,
Accord ing to Raper a nd Thom ( 1949) th e tw o species sh ould be regarded as
close ly rel ated , a n d as p ossibly forming a bridge between th e M on overticillata
a nd th e Penicill ium citrinum series.
10 urn
iR.
P /Jr
c-L
100 J.lm
Penicillium charlesii Smlth - Trans. Brlt. Mycol. Soco 18: 90-91. 1933. See also
Raper and Thom, 'A Manual of the Penicillia', pp. 248-250. 1949.
Colonies upon C A (colour plat c 70 , A and a) att ainin g a diam eter 01' 38 mm
in 14 days at room temperature (20- 25 OC), consisting 01' a tough , closetextured basal felt , deepl y velve ty in a ppeara nce to sublanat c , strongly rai sed
in th e ce ntre up to 3 mm deep, with a depression in a crate riforrn pattern ; eve n
m a rgin su rro unde d by a fringe 01' submerged hyphae exte nd ing about 2 mm
beyond th e ae rial growth; colony su rface radiat ely furrowed, rem aining
persistently sno w white throu ghout ; m edium sporing throughout , heavier in
submarginal are as, in a rte m isia green shade s (S guy, PI. XXIX , 425) , not
influencing th e gener al colony appearance; exudate and odo ur lacking; no
coloure d pigmen t diffu sin g into th e su rro u nd ing agar; colony reverse (colour
pIate 70, a) strong ly pulling away and cracking th e ag a r, allowing th e air to
pen etrat e underneath and a good sporu la tion, othe rwise colourle ss; peni cilli
sim ple (fig. 70, A), with stipes arisin g from cre eping or closely interwoven
ae rial hyphae , sim ple or variou sly ramified , occasionally showing fairly
com pact clus te rs 01' m etula-like branch es , eac h ramification bearing a
monoverticillate peni ciJlus ; th e stipe s 01' th e conid iopho res are variable in
length , ranging from very sho rt up to 100 Ilm or more by 2.0-4 .0 ust , with
apices enlarged in a ves icle up to 4.5 Ilm in di amet er, smooth wall ed (r eported
as slightly rough en ed by G . Smith) ; phialides in com pac t clus te rs, m or e or less
parallel , numbering 6- 12 eleme nts in the ve rticil, m ostl y 8-10 Ilm by
2.9-4 .0 psi , terminating abruptly in a short-pointed neck ; conidia ovate to
su bglobo se, deli cately roughened (Fig. 70 , B, and SEM pIate 12, ce ntre:
right) , mostly 3.0- 4. 0 psi: by 2.5-3 .0 tui ; conid ial chain s producin g welldefin ed colu m ns (fig. 70, C) .
C olonies upon CYA (colour plat e 70, B and b) attain ing a di amet er 01'
28 mm in 14 days at room temperature (20-25 OC ), consisting al' a tough ,
close-t extured bas al felt , velv et y in appearance , overlaid by a thin mycelial
ove rg ro wth 01' white , ste rile, closely interwoven ae rial hyphae, influen cing
gre atly th e colony appe arance; colony su rface strongly wrinkled , sulcated by
numerou s, deep , radial furrows , with centre raised in a crateriform pattern ;
undulating margin surrounded by a narrow fr inge a l' submerged hyphae
exte nd ing less th an 1 mm beyond th e aerial gro wth; colonies strongly pulling
away and crac king th e a ga r , remaining persistently white owing to th e thin
m ycelial ove rgro wth ; slightly spo ring throughout , not influen cing the colony
appearance ; odour lacking ; exudate not obs erved ; amber- coloured pigm ent
diffusin g into th e su rrou nd ing agar; colony reverse (colou r plate 70, b) in
cinn namo n shades ; peni cilli essentially as described aboye.
C olonies upon M EA (colour plate 70, C and c) attain ing a di ameter 01'
45 mm in 14 days at room temperature (20-2 5 OC), cons isting 01' a close-
195
textured basal fclt , co ns picuous ly zonu tc, slig hlly raised and sulca ted by
sha llow radial Iurrow s, a p pearing vclvery, with thin , even rnargin surrounded
by l wide frin ge 01' su b rne rge d hyphae cx ic ud ing 5 mm or m ore beyond the
ncr ial gro wth; colony su rfacc rcmaining pe rsi ste n tly snow white in colou r;
spo ring spa ringly, not influen cing the co lo ny as pec t; od ou r faint , indefini te;
cxudate lackin g; pale a m be r-c oloured pi gm ent diffusing into th e su rro u nd ing
agar; colo ny rever se (colour plat e 70, c) in pal e cre a m sh ad cs; pcnicilli
essc n tially as d escr ibed aboye but with stipe s arising mostly frorn su b me rgc d or
ascc nd ing ae rial hyphae , co m monly 200 ,tm or m ore in len gth , loosely
ramified , with penicilli u su all y well separated a nd p roducin g co nid ial chains in
lon g narrow well-defined colu m ns 100- 200 ,tm in len gth .
T he species d escription is ce n te re d upon th e typ e strain CBS 304 .48 = C M I
10,232 = AT C C 8, 730 = NR RL 1,887 = Q M 6,83 8 = IJFM 3756, isolat ed from Ital ian
mai ze (Zea mays L. ), by G. Sm ith in 1932.
St ra ins showing th e general cult u ral characte ristics a nd details al'
morphology 01' Penicillium charlesii Smith a re occasionally isolat ed from soil an d
[rorn organic su bs tra ta ex pose d to soil co ntam inatio n, a s fabrics, tents am i
othe r military equipmc n t undergoing deterioration in trop ical a nd su btro pica l
zones .
fR.,
\':\1
l.--.....J
100 jirn
1 ~I I
o 0 0
O O O
OO
O O
O
00 () Q
OB
00
O
O
O
~
10
jrrn
)11'1"1
L--......J
100
jirn
fR.
ramificati ons some times resembling apica l ve rticils of m etul ae , eac h te rrnin ating in a m onoverticillate penicillu s (fig . 72, A) ; phialides in clu sters of 3- 8
ele me nts in th e vertici l, mostly 9 - 13 p.m by 2. 4-3 .8 psi , te rm in ating in a
sho rt-po inted narrowed neck ; conid ia ellip tica l to su bg lobose, m ostly
2.5-3.5 p.m by 2.0-2 .5 p.m in di ameter, with walls smooth or nearly so (fig.
72, B, a nd SEM pIate 12, bottom: ri ght) ; co nid ial chains forming par all el or
loose colum ns (fig . 72, C ) .
Colonies upon CYA (colour pIate 72, B and b) a ttain ing a d iameter of
40 mm in 14 days at room temper a ture (20 - 25 OC), cons isting of a closetextured basal felt, deepl y velvet y , ra di ately wrinkled, with ce ntre depressed in
a crat eriform pattern ; eve n m argin surro u nde d by a ve ry narrow fringe of sub merged hyphae exte nd ing less th an 1 mm beyond th e ae rial gro wth; heavil y
sporing in subcentral are as in deep blue-gr een shades (S guy, PI. XXX , 44 7);
non-sporulating areas remainin g sno w-wh ite in colou r; exudate lacking; odo ur
faint , indefinite ; amber-col oured pigment di ffusin g in to th e surro und ing agar ;
colony reverse (colour pIate 72, b) in yellowish shades; penicilli esse nt ially as
descr ibed aboye .
I ~) \
violaceou s, showing little spots deep red in colour, w ith centr e in dark brown
shades. Penicilli simple, wi th tangled chains of conidia. Conidiophore of the
ramigena type , smooth walled , 10.0-30.0 (60.0) .tm by 2.0-2.5 .tm, with apices
inflated; phialides in verticils of 5-8 elements, slightly divergent, 6.0-9 .0 (11 .0) .tm
by 1.8-2.2 .tm. Conidia subglobose, smooth walled , 2.0-2.5 .tm. It belongs to the
Monoverticillata-ramigena. No related species are known.
Type: isolated from stored grapes, in the Alma -Ata region , USSR, 16-X-1967 by
T.1. Novobranova.
(Translated from the Russian by Prof . L. Vilas).
"'/1
hapt cr 4
The Asymmetrica
RAP ER ANO THOM (1949) IN CLUOEO I N THI S SE CTI ON AL L T H E PE NI CILLI A WIII GII
pr oduced two- or three-stage branched penicilli , in whi ch th e ramifi cation
sys te rn was typically irregular, on e side d , or asymmetrical.
T he ge ne ra l typ e 01' penicillus d escribed is 01' an arb itra ry characte r,
co rnmon to seve ra l more or less well-defined groups , co nside re d by th e authors
mentioned as su b-sectio ns . Each 01' th ese su b-sec tions em b raced two or more
se ries whi ch were beli ev ed to represent natural grou ps 01' spec ies. Fiv e su bscctio ns we re recognized and are m aintained here as fol1ows:
DIVARICATA . Charact eri zed by th eir stro ng ly divaricat e or bi fur care
penicilli , co m mo nly presenting th e a ppe ara nce 01' irregular and a pical clus te rs
01' penicilli ret aining alw ays th eir monoverticill at e characte r.
VELUTINA. Grouping spe cies producin g penicilli one - or two -stnge
branched , es pecially characte rized by th e producti on 01' ab u ndan t co nid ial
struc tu res with th e stipes 01' th e con id iop ho res a rising primarily in a dense
stand from th e substratum or the ba sal felt , giv ing a velvety a ppeara nce lo th c
colony surface.
LANATA . G rouping species producing penicilli similar to th e preceding
gro u p, but charac te rize d by th e producti on 01' a loose-textured network 01'
ae rial hyphae , from w hich a rise largely the stipes 01' th e conid iophores,
pr esentin g a lan at e or floccose overa ll as pec t.
FUNICULOSA. Grouping species producing penicilli , as previ ousl y , but
with th e m yceli al ove rg rowth sh owing definite aggre ga tes 01' m yceli a or rop es 01'
hyphae or fu nicles, with th e stipes 01' th e con id iophores com m only arising a s
shor t branch es from su ch furrieles .
FASCICULATA. Grouping species producing penicilli sim ila r in pattern to
th e preceding , but arising ch aracteristicall y from syn ne mato us co nid iop ho re s.
ind cfinitc numbcr 01' b runch es arising' irre gui arly at lowc r lcvclsto co mprise a
looscly a rrangcd two-st uge branc hcd a nd asym me tric pcni cillu s. In a nu mbcr
01' spee ies th e br an ch es arisc at onc Icvcl in a mctula-likc m anner , comprising
fairly eom paet but definitely d iva riea te pen icilli .
M embers 01' th e gro up a re eom manly isolat ed frorn soil, vege ta ble rem ains,
a nd or ganie mat eri als in th e lat er stages 01' decompositi on. They a re fre q ue ntly
eneo unte re d on fab ries a nd othe r military equ ipme nt undergoin g det eri or ati on
in th e field.
Altho ug h the Asymmetriea- diva rieata are re garde d as rep resen tin g, in
gene ral , a na tural grou p 01' Peni eillia , th ey sho uld be eons idered, however , as
oeeupy ing a somewhat intermedi at e posit ion in th e genus as a who le , sinee
different m embers assigne d here show affinities to ot he r well-defined gro u ps .
In eonst rueting th e key , we had to eree t th ree add ition al series to
aeeomo date seve ral 01' th e numerous spee ies 01' D iva rieata deseribe d sinee 1949
th at could not satisfaeto rily be c1assified in any 01' R ap er and T ho m' s ( 1949)
kn own series, nam ely:
T he P. godlewskii series, inc1uding th ose form s gro wing rather restrietedly,
with penieilli praduein g st ro ng ly diver gent eo nidial eha ins, a nd eomparatively
small eonid ia .
T he P. atro -sanguineum series, ereete d to inc1ude those for m s developing,
up an sorne substra ta, deep pu rpl e 0 1' blood-red eolou rs in th e colony reve rse,
an d diffu sin g int o the surrou nding agar .
The P. brasilianum series , inc1uding form s spreading broadl y on all subs tra ta,
appearing deepl y floeeose .
P. rolfsiiThom . . . . 217
b. Blaek sclerotia reported .
. . .. 233
.. . . 236
. ... 238
.. . . 243
. . .. 254
. .. . 240
. .. . 246
2''' , Conid iophoros consplcuously w arty; panicllli irregular; conidia olllptlco l, smoot h walled or delicately
roughened; colony reversa developing orange
colours .
P. ochro-chloron Biou rge ... . 248
3'''. Conid iophores smooth walled or nearly so; penicilli
commonly irregular; conidia mostly subglobose,
conspicuously echinulate; colony reverse in cream to
light shades.
P. piscarium Westling . ... 250
4' '' . Conid iophores smooth walled or nearly so; penicilli
irregular; conid ia elliptical to subglobose, smooth
walled or nearly so.
aaaa. Conidia comparatively large, up to 6.5 {t m in
diameter; phialides large, up to 19 {t m in length.
P. aragonense Ramrez et Ma rtnez . .. . 256
bbbb. Conid ia compara tively small , up to 2.5 {t m in
long axis; phialides small , up to 10 {t m in length.
P. miczynskiiZaleski . . .. 262
bb. Phialides not abruptly taper ing to a long, narrowed neck;
penicilli cons isting of c1uste rs of strongly divergent phialides,
producing divergent columns of conidia; conidia compara tively small, usually not surpassing 3.0 {tm in diameter.
P. godlewskii series ... . 258
1". Conidiophores smooth walled or nearly so.
aaa. Conid ia smooth walled or nearly so.
1' ", Colonies growing very restrictedly upon Czapek's
agar, attaining up to 20 mm in diameter.
aaaa. Colon ies appearing definitely funiculose.
P. godlewskiiZaleski . . . . 259
bbbb. Colon ies appearing velvety
P. eben-bitarianum Baghdad i . . . . 266
2''' . Colon ies atta ining a diameter of 30 mm on CA .
aaaa. Conidia comparatively small, up to 3 {t m in diameter.
1"". Exudate and reverse in deep orange shades .
P. gorlenkoanum Baghdadi . . . . 269
2' ''' . Exudate colourless and reverse yellowish to
colourless.
P. botryosum Batista et Maia . .. . 261
bbbb . Conidia larger, up to 4 {t m in diameter.
P. citreo -virens Abe ex Ramrez ... . 279
.... 273
. . .. 271
. ... 268
.. .. 275
. .. . 264
. . .. 283
. . . . 285
... . 287
.. .. 293
.. .. 291
.... 289
C'OIllIlHJIl ly IIp to 2:">()- 1-00 u n 01' ruorc in lcn gth I with walls ra llging' rom
u ns picou sly cc h in uln tc lo smooth 01' ncurl y so .
Pcn icilli typ ica lly d ivari cat c usyuunctri cul, usua lly co nsisting' 01' a term inal
ve r!icil 01' 2 - 5 m ctu lac, gc nc ra lly som cwhut di vcrgcnt .
C o n id ia glob osc lo cllip tica l , with wa lls gcnerally smooth 0 1' ncarly so.
K cy lo spccies
Paga
A . Colony surfaee velvety or nearly so upon most substrata ; stipes arising
direetly from the substratum or from aerial hyphae.
P. raistrickii series
1. Conidiophore walls eoarsely roughened ; sclerotia well developed,
very hard .
a. Sclerotia very hard , stony, white to pale pink in eolour; vegetat ive
hyphae always eolourless.
P. raistrickiiSmith
b. Sclerotia fairly firm , yellow to ligh t brown in eolour; vegetative
hyphae not always eolourless.
1' . Vegetative hyphae sometimes enerusted with yellow granules;
con id ia subglobose, up to 4.5 JLm in diameter.
P. pulvillorum Turfitt
2'. Vegetative hyphae always enerusted w ith orange granules;
eonid ia elliptiea l, up to 3.5 JLm in the long axis.
P. pedemon tanum Luppi-Mosea et Fontana
2. Conidiophore walls delieately roughened , true sclerotia laeking; small
rounded masses of th iek-walled eells present upon all substrata and
partieularly upon malt extraet agar.
P. soppiiZaleski
3. Condiophore w alls smooth or nearly so.
a. White to pink sclerotia reported .
P. rolfsiiThom
b . Blaek sclerotia reported.
P. mirabile Beljakova et Milko
e. Small masses of thiek-walled eells (as in P. soppii) produeed in
some strains .
P. micz ynskiiZaleski
(see P. janthinellum series)
. . . . 208
. . .. 210
. . . . 212
. .. . 221
. . .. 214
.. . . 217
. . . . 224
.. . . 252
th c lorrnation 01' synne mata. In add ition lo th c our specics cons ide red hcre by
Raper and Thom (191,9), namcly: Pen icillium raistrickii Sm ith , P. puloillorum
Turfitt, P. soppii Zale ski, and P. rolfsii Thorn , th c followin g spccies havc been
included in the seri es in the prescnt study : P. pedemontanum Luppi-Mosca et
Fontana, and P. mirabile Belj akova et Milko . Howevcr , a close relationship
between th ese forms is not presumed here , a nd th e six species are conside red
together principally as a m atter 01' conve n ience.
The sclerotia 01' sclerotia-like cellula r mas ses , differ su bsta ntially, dep ending
upon th e species. In Penicillium raistrickii th ese are globose to su bg lobose , ve ry
hard a nd gritty , be aring a striking resemblance to th e flesh- to pink-coloured
scle ro tia produced by P. thomii Maire , in th e Monov erticillat a Section (colou r
pIate 1). The cellula r masses in P. puloillorum are less regul ar in shape, 01't en
larger, firrn , but not sto ny, com monly su rrou nde d by a loos e network 01'
yellow- to brownish- en crusted veget ati ve hyph ae lending a brownish colouration to the colony su rface . In P. pedemontanum, th e scle ro tia are hard , alw ays
surrou nde d by a loose network 01' fin e orange-enc rus ted hyphae whi ch lends a
deep orange colou ra tion to th e colony . P. roljsii Thom a nd P. mirabile Belj ak ova
et Milko are in cluded in th e series primarily because Thom (1910 ), in his
origin al di agnosis 01' P. roljsii reported th e production 01' white to pink sclero tia,
a nd Belj akova and M ilko (1972 ), in th eir or iginal diagnosis 01' P. mirabile,
rep orted the presen ce 01' hard black sclerotia . On th e othe r hand , the aggrega tes 01' thick-w alled sterile cells ob serve d in P. soppii are qu ite unusu al amo ng
th e Pen icillia , suggesting rudimentary sclerotia, 0 1' possibl y asc omata . T hey
are regul arly produced adjacent to th e su b stratum a nd are generally obsc ured
by the m ycelial ove rgro wth a nd th e heavily spo ri ng a reas.
73 . Penicillium raistrickii Smith : fig . 73, colour plat e 73, and SEM plat e
13 , (t op : left).
Penicil/ium raistrickii Smith - Trans . Brit. Mycol. Soco 18: 90. 1933. See also Raper
and Thorn, 'A Manual of the Penicillia', pp . 275-277. 1949.
Smith's diagnosis is as follows:
'Colonies growing rapidly on most media at temperatures up to 30C, rate of
growth much less above 30C; bluish green, then grayish green , and finally some what brown , velvety; reverse persistently uncoloured, showing masses of sclerotia;
conidiophores arising form the substraturn, up to 250 .tm long, 3.5-4.0 .tm in
diameter, rough; penicilli typically divaricate, comprising 3-5 monoverticillate
heads united into a fairly compact whole; metulae clavare, sometimes very slightly
roughened, 10-13 .tm by 4.0-4.8 .tm; phialides closelv packed on each individual
metula, 7 .5-9.0 .tm by 2.6-3.0 .tm; conidia globose or nearly so, smooth, 2.2-2.7
.tm in diameter, conidial chains packed into diverging salid columns; sclerotia
....
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produced abundantly, hard, feeling like grit under a cover glass, consisting of
masses of irregular-shaped thick-walled cells , white to dirty wh ite, globose or ovoid ,
up to 180 JLm in long axis.'
sclcrot iu are ab un dantly prod uced rhroughout thc colony surfacc , often
characterizing ils ap peara nce .
Colonies up on CYA (colour piale 73 , B a nd b) auain ing a diarnetcr 01' 60
mm in 14 days at room temper atu rc (20 - 25C), duplicati ng in tcxtu rc ami
appearance those grown up on C A, but radiatcly sulcat ed by numerou s and
deep furro ws, with cen tre ra ised; margin su rro unde d by a wide fringe 01'
submerged hyphae , ex te nding about 4 m m beyond th e aerial gr owt h ; spor ing
ab undantly in marginal areas in the same shades as described aboye; ex udare
abundantly produce d, crystal c1ear, colou rless; odour lacking ; no coloured
pigme nt diffus ing int o the surroundi ng agar; colony reverse (colour pla te 73,
b) in orange shades; penicilli essential1y as describe d aboye .
Colonies upon MEA (colour plate 73, C and c) attaining a diameter 01' 60
mm in 14 days at roo m temperature (20 -25C), consisting ofa loose-textu red
basal felt overlaid by a Ioosely woven network 01' floccose aeriaI hyphae
in fluencing greatly the colony appearance, plane; heavily sporing in margi na l
areas in blue-green shades (Sguy, PI XXXI, 46 1); exudate and odo ur
lacking ; sclerotia Iess ab undantly produced than upon the precedi ng substra ta;
colony surface appearing light buff from the production 01' sclerotia and the
abundant ovcrgrowth 01' sterile floccose hyphae ; no coloured pigment diffusing
into the surrounding agar; colony reverse (colour plate 73, c) in reddish brown
to deep orange red shades; penicil1i essential1y as described aboye . (Fig. 73, A
and C).
The species description is centered u pon the type strain CBS 26 1.33 = CM l
40 .22 1 = ATCC 10,490 = NRRL 2039 = n-o 6, 104 = QM 1,937 = VKM F-337, isola ted
by George Sm ith fro m mo uldy cotton yarn in 1927, in England .
~ llf lf@'~
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r~
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f
e .
100 prn
I~,
~.
giving' a lan utc appl:a ranee lo 11H: colony, wh ich rc ma ius pcrsistcnrl y whit c I'Osy
in colou r; oven ma rgin su rrou nde d by a frin gc of sub mc rged hyph ae cx ic nd ing
about 1-2 mm bcyond thc aerial growth; slighlly spo ring in palc blu c-gr een
shades (S guy , PI. XXX, 444); odour lacking ; ex udare in large , crys tal-clear ,
un coloured drops ; pal c am ber-colou red pigment diffu sin g in to the su rro unding
ag a r; colony rever se (colour plate 74 , a) in pal e ora nge shade s; peni cilli as
described by Turfitt (fig. 74, A) , with conid iophores cons picuous ly and finc1 y
roug h wall ed , arising mostly from tr ailing hyphae ; co nidia elliptical to
subglobo se (fig . 74 , B, and SEM plate 13, top : right) , with walls smo oth 01'
nearl y so, 3 .0 - 4 .8 /lm by 2.5-4.0 /lm in diamet er. In ou r expe rience , the
scleroti a 01' P. pu loillorum , whil e not hard and gritty , are nevertheless quite firm
and withstand cons ide rable pressure without collapsing .
Colonies up on CYA (colour plat e 74 , B and b) attaining a diamet er 01' 55
mm in 14 days at room temperature (20 - 25 C), duplicating in texture , cultu ra l cha racte ristics, a nd details 01' morpholo gy thos e grown upon CA ; even
margin su rrou nde d by a narrow frin ge 01' submerged hyphae exte nding ab out 1
mm beyond th e aerial gro wth; heavil y spo ring in m arginal areas in gray-gree n
shades (S guy, PI. XXVII, 403); odo ur lacking ; exudate abu ndantly produced
in large , amber- coloured drops ; pale amber-coloured pigm ent diffu sin g into
th e su rrou nding a gar; colony reverse (co lour plat e 74, b) in pal e or ange
shades ; peni cilli and scle ro tia as described aboye .
C olonies up on MEA (colour plate 74 , C a nd c) attaining a dia me ter 01' 45
mm in 14 days at room temperature (20 -2 5C), cons isting 01' a den se layer 01'
larger , yellow to light brown sclerotia , up to 250 /lm in long ax is (fig. 74, D ),
ove rlaid by a loose network 01' interw oven hyphae ; spo ring heavily th rou ghout
in mouse gray shades (Sgu y , PI. XXIX , 430) ; with a n eve n margin
su rr ou nde d by a fringe 01' subme rge d hyphae exte nding abo u t 2 mm beyond
the ae rial growth; odour and exuda te lacking ; on coloured pigm ent diffusing
into th e surrounding agar; colony re ve rse (colou r plat e 74, c) in pale cream
shad es; penicilli esse ntially as described ab oye but with conidiophores arising
mo stly fro m th e substratu m, com mo nly 500 /lm 0 1' m or e in len gth by 3.0- 3.5
/lm in di amet er; phialides in clu sters 01' 3- 8 elements in th e verticil , mo stly
7-11 /lm by 2.5- 3.5 utti, terminating in a conspicu ou sly taper ed , narrowed
ne ck (fig . 74, A); conid ia appearing deli cately roughen ed ; conidial cha ins
tangled 01' formin g divergent colum ns (fig . 74, C) ; sclerotia up to 250 /lm in
diameter.
The sp ecies description is cente red upon th e typ e strains C BS 280. 39 = NR R L
2,026 = IFa 7,763 = IJFM 5118, isolat ed by G .E . Turfitt from ac idic soil
collected in London , U .K.
75. Penicillium soppii Zaleski: fig. 75, colou r plat e 75, and SEM pIate 13
(centre: left) .
WJ..W.u.J
10 jrrn
o~
00~
O 000
O
00
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00
80
,,/
~ l?# 1~ 1~/ lR
o
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1~ 1
.1lc
Penicillium soppii Zaleski - Bull. Acad. Polonaise Sci. Math. Nat. Sr. B, pp.
476-477.1927. See also Ch. Thom, "The Penicillia ', pp . 344-345.1930; also Raper
and Thom, 'A Manual of The Penicillia', pp. 279-282. 1949.
Synonymy
Penicillium matris-meae Zaleski - Bull. Acad. Polonaise Sci. Math. Nat. Sr. B,
pp. 477-479. 1927.
rcddish sic n na brown shadcs: with rhick-wullcd, stcrilo ce lls in small m a sses u!'
to 50 -60 J.III in diam etcr, suggcs tiug small sclcro tia , occuring adjaccn t to th e
subs tra turn th rou ghout th e colony but rnos t ab undan tly alo ng thc inter colony
margins, resembling fine white sa nd in appearancc but not firm o r gritty ;
peni cilli (fig. 75, A) borne up on lon g con id iopho res with walls smoo th or finel y
roug he ned, up to 1000 J.m by 2.5-3 .0 uiri , arising mostly from the substratum
along the intercolony margin s, a nd as bran ches of va riable len gth from ae rial
hyph ae in centra l are as; penicilli forming large struc tures usu ally consisting of
a terminal verticil of 2-5 metulae , 12- 25 J.m by 2.5 -3. 0 uu , with ap ices
enla rged in a ves ic1e up to 4.0 J.m in di am ct er; phialidcs in c1us ters of 2-8
closely crowded eleme nts in th e verticil, mostly 9- 15 J.m by 2.5 -3 .0 tui,
terminating in a short-po in tcd ncck ; conidia elliptical to subglob osc (fig. 75, B,
and SE M plat e 13, ce nt re: left), mostl y 3 .5 - 6.5 tut: by 3. 0- 4.0 J.m in
diameter , with su rface smooth or delicatel y ro ug he ned; conidial chains loose to
tan gled .
Colon ies up on C YA (colour plate 75, B a nd b) broadly spreading , attain ing
a di amet er of 60 mm in 14 days at room temperature (20-25C), cons isting of
a c1ose-textured basal felt , ove rla id by a loosely interwoven netw ork of floccose
ae rial hyph ae lending a lanate appeara nce to the colony su rface, which is
deepl y radi at ely furrowed ; heavily sporing particul arl y in m arginal areas in
blue-green shades (Sgu y , PI. X X X, 438) ; thin , eve n marg in surrounde d by a
wid c frin ge of submerged hyphae extend ing 4 -5 mm beyond th e ae rial growth;
odo u r lacking; exudate ab undantly produced in pale yellow drop s; very pale
am ber pigment diffu sin g into the su rrou nding agar; colony revers e (colour
plat e 75, b ) in cinnamon shades; penicilli esse ntia lly as described aboye , with
the conidiopho res arising m ostl y from tr ailing ae rial hyphae .
Co lon ies up on M EA (colour plat e 75, C and c) at ta in ing a diamet er of 60
mm in 14 days at room temperatu re (20-25C ), cons isti ng of a deepl y velvety
to subla nate felt , slightly zanat e in central arcas, pla ne , ab undantly spo ring
throu ghou t in pale blu e- green colours (Sguy, PI. XXVIII , 4 15); thin m argin
surrou nde d by a frin ge of submerged hyph ae exte nd ing 4-5 mm beyond th e
ae rial growth; exuda te a nd odo ur lacking ; no coloured pigm ent diffu sin g into
the su rro u nd ing agar; colony reverse (colou r plate 75, e) in pale yellow sha des;
peni cilli esse ntially as described aboye, but with the stipes of the conidiophores
arising mostly fro m th e su bs tratum an d from ae rial hyphae .
The spec ies description is cen tered upon th e type strain CBS 226. 28 = C M I
40,2 17 = ATCC 10,496 = NR RL 2, 023 = I Fa 7,766 = QM 1,964 = IJ FM 5126,
isolat ed by K . Zaleski from soil in Poland , 1927 , and up on strain NR RL 9 12 =
C BS 225.28 isolated fro m th e sa me sou rce by Zaleski in the sa me year , and
m aintained in artificial cultu re since 1928 in th e Northern R egional R esearch
Laborat ory , Bureau of Agricultural and Industrial Chemistry, U.S . D epart
m ent of Agriculture , Peoria , Illinois. However , although it duplicates th e
76. Penicillium rolfsii Thom : fig. 76, colou r plate 76, a nd SEM plate 13,
(centre: right).
~50 ~
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00 O O O
00
Oo
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O
O
.
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,: ,~' e f. {.~'$
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"
-ti
100 prn
lwlJ.1w.J
10 prn
Penici//ium rolfsii Thom - Bul!. Bur. Anim. Ind. U.S. Dep. Agric. 118: 80-81. 1910.
See also Ch. Thom, 'The Penicillia', pp. 489-490.1930; also Raper and Thom, 'A
Manual of the Penicillia', pp . 282-283. 1949.
Author's original diagnosis follows:
'Colonies upon milk -sugar-gelatin and potato or bean agars gray-green;
floccose, but with aerial part mostly long conidiophores and few vegetative hyphae,
slightly yellowish to pronounced salmon colour below; broadly spreading;
developing elliptical to globose scierotia 150-200 p'm in diameter at the surface of
the substratum in 2-3 weeks, conidiophores 200-500 p'm by 3-4 p'm; penicilli in
verticils of 3-5 branches Irnetulae) 10-17 p'm by 2-3 p'm, rarely secondary verticils
each bearing a dense vert icil of sterigmata (phialid es), 8-10 by 2 J-lm , produclng
long , parallel, or slightly divergent chains of conidia; conidia elliptical or fusiform,
3.5-6 J-lm and producing one to several germ tubas . Colonies slowly Iiquefy
milk-sugar gelatin and produce purple to neutral colours in Iitmus media.
Sent by Prof. P.H. Rolfs from Miami, Florida, on portion of pineapple , March,
1905.
Colour gray-green, with scattered white to pink sclerotia, reverse sulphur
yellowish to pronounced salmon; colour in media reddish or yellowish in special
cases, others none. Odour none.'
O u r notes follow :
Colon ies upon C A (co lour plat e 76, A and a) a tt a ining a di ameter of 65 mm
in 14 days at room temperature (20- 25 C ), cons isting of a close- textured basal
felt , overla id by a den se network of floccose wh ite ae rial hyph ae , lendin g the
colony a lanate appeara nce; th in, eve n m argin su rrou nde d by a wide fringe 01'
submerged hyphae exte ndi ng abo u t 3-4 mm beyond th e aeri al gr owth ; heavily
sporing throu ghout in gray-green to blu e-gr een shades (Sguy, PI. XXX , 449,
442), with con id ial areas partially obsc ured by the den se overgrowth of floccose
ae rial hyphae , espec ially in sub-ce ntra l a nd m arginal a reas, show ing whitc
rosy sha des in the colony su rface ; odo u r a nd exudate lacking; coloure d
pigm ent d iffus ing into th c su rrou nd ing aga r not produced ; colony revers e
developing salmon pink colours (colou r plat e 76, a); peni cilli are irregular in
pattern and essentially as described by Thom ( 19 10), but showing also sim ple
pen icilli (fig . 76, A) , with th e stipes 01' thc conidiopho re s u su ally smooth
walled , but som etimes show ing some what rou gh walls; phialides terminating
in a sho rt, tap er ed neck ; conidia strongly ellip itical, with walls smooth or
nearl y so wh en observed under the light mi cro scop e (fig . 76, B), but delicately
gra nular wh en ob serv ed under th e SEM (pl a te 13, cen tre : rig ht), mo stly
3.0 - 4.0 p.m by 2.0 -2 .8 p.m in di am et er; conidial chains loosely parallel (fig.
76, C ) . Scle ro tia wer e not obse rve d .
Colonies up on C YA (colour plate 76, B and b) broadl y spreading, attain ing
a di amet er of 65 mm in 14 days at room temperaturc (20 -2 5C) , duplicating
in cultu ral charac teristics a nd details of m orpho logy those gro wn up on C A, but
with colony su rface deepl y furrowed in a radial pa tte rn; spor ing sim ilarly as
abo ye in th e sa me shades; exudate and odour lacking; no colou red pigm ent
diffu sin g into the surrou nding medium ; colony reverse (colour plate 76, b) in
deep er salmon pink shades; peni cilli essentially as described aboye but with
conid ial chains tan gled. Scle ro tia wer e not observed .
C oloni es upon M EA (colour plate 76, C and e), attaining a diameter of 65
mm in 14 days at room temperature (20 - 25OC ) , consisting of a close-t extured
basal felt overl aid by a den se netw ork of whi te rosy floccose aerial hyphae ,
obscu ring th e otherwis e heavily sporing areas, particul arl y in central and
subce ntral zon es, influ en cing greatly th e colony appearance, wh ich devel op s
whit c rosy shadcs in ce ntral and subccnt ral arcas ; hcavily sporing arca s in
margin dcvcloping decp blu c-grccn sha des (Sguy, PI. XXX, 441); odour a nd
cxudate lacking; no coloured pigment dilTusing into th e surrounding aga r ;
colony revcrse (colour pIate 76 , e) developing yellowish to pale salm on pink
shades; peni cilli essentially as described aboye . Sclerotia were not observe d.
Colonies grawn upon th e three standard su bs trata us ed in th e curre nt study
conforrn fairly closely with Thoms (1910) original des cription but differ in its
Iailure to produce sclerotia .
W e a gr ee with R aper a nd Thom (1949) in ass ign ing thi s species to th e
Penicillium raistrickii series in th e Asymmetrica-divaritaca su b-sec tion , on
account of th e reported presen ce ol' white to pink sclerotia 150- 200 p.m in
di ameter in th e type strain when originally described.
The species description is ce nte red upon th e typ e strain CB S 368 .48 = C M I
40, 02 9 = ATC C 10,491 = NR R L 1,078 = IFa 7,785 = Q M 1,961 = IJFM 5119 ,
isolat ed by Ch . Thom from a portion of A nanas sativus sent by Prof. P .H . R olfs
frorn Miami , Florida , M arch, 1905 .
00
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Penicillium ro/fsii Thom, varo sclerotiole Novobronova - Nov. Svst, Nizhnlkh Rast.
11: 230-232. 1974.
Novobranova's original diagnosis follows:
'Colonies upon Czapek's agar attaining a diameter of 25 mm-30 mm in 10 davs,
with undulating margin; close textured , with the colony centre somewhat heaped;
furrowed; grayish violet-coloured sclerotia abundantly produced throughout the
colony surface in 34 days, elliptical to subglobose or irregularly shaped, mostly
200-350 .tm or more, up to 400 .tm in diameter. Conidial structures appearing slowly
and sparingly, particularly in submarginal zones, developing gray-green shades;
exudate abundantly produced, principally in submarginal areas, as crystal c1ear,
hyaline droplets. Colony reverse wrinkled in the centre, whitish, with centre in
marble pink colours; strongly splitting the agar underneath. Conidiophores with
walls delicately roughened, 200-500 .tm by 3.0-4.0 .tm. Penicilli borne on c1usters
of metulae, numbering 3-5 elements in the verticil, mostly 10.0-17.0 .tm by 2.0-3.0
.tm; phialides measuring 8.0-10.0 .tm by 2.0 .tm; conid ial chains long, either parallel
or divergent . Conidia smooth walled, elliptical, mostly 3.5-4.0 .tm by 2.0-3.0 .tm in
diameter. The species has been assigned to the Penici/lium raistrickii series, in the
Asymmetrica-divaricata sub-section. It is closelv related to P. ro/tsii Thom, from
which it differs particularly by its morphological characteristics and by the abundant
production of sclerotia upon Czapek's agar.' (Translated from the Russian by Prof.
L. Vilas).
hyph ae extc nd ing abou t 2 -:~ 111111 beyond the acrial growth ; ce ntral and
subcc ntral arcas deep ly radiately lurrowcd: heavily sporing throughout in
blu e-grecn shades (Sgu y, PI. XXXI, 463) ; exu date and odour lacking; no
colourcd pigment diffusing into the surrounding agar; colo ny re vers e (colour
pial e 77, b) strongly pu lling the agar away , developing brown colours : penicilli
esscntially as described aboye; sclerotia not observed upon this substratum .
C olonies u pon MEA (colour plat e 77 , C and c) growing rather restrictedly,
consisting 01' a looser textured basal felt, vel vet y in appearance, attaining a
diameter 01' 45 mm in 14 days at room temperature (20-25 C) , plane,
sornewha t zonat e , wit h margin irregu larly disse cted surrounded by a fringe 01'
su bmerged hyphae extending about 1- 2 mm beyond the aerial growth; heavily
sporing throughout in a rte m isia gr een shad es (Sguy , PI. XXIX, 430) ;
exudate and od our lackin g; no coloured pigment diffusing into th e
surrounding agar; colony reverse (colour plate 77 , e) in yellow-orange shades ;
pcnicilli esscntially as described aboye; sclerotia have not been observed upon
this substratum .
The spec ies des cription is centered upon the type strain C BS 752 .74 = VKM
F- 1815 = ATCC 24 ,724 = IJFM 7038, isolated from the surface 01' fruit 01' Pirus
malus L. variety 'Renet Burchardt' in the Alm a-Ata region 0 1' the S .S .R. 01'
Kazachstan, USSR , by T .I. Novobranova, April, 1968. It is known on ly from
the typ e spe cies .
,:" ,
~;@,;: \"'.",;:
,t
"
:8:@
GJ\'\@ . .
'*::{try
l......---J
100 prn
.R.,
to deep olive, copper yellow in marginal areas, then (after 3 weeks) becoming deep
brown coloured, with growing margin narrow and uncoloured; exudate Iimited in
amount, deeply enmeshed in the mycelial felt in central zone, colourless; colon y
reverse strongly wrinkled in the centre and slightly furrowed in marginal area,
developing bright chamo is shades, then becoming black with the production of
sclerotia in central area, (atter 4 weeks) becoming black in the centre and in some
marginal spots. The conidiophores are sparsely produced, septate, up to 500 ~m in
length by 2.3-3.5 ~m in diameter, with apices enlarging in a vesicle up to 4.0-4.5
~m in diameter, arising either from the substratum or from aerial hyphae as short
branches, mostly 10-40 ~m up to 150 ~m in length by 1.5-2.5 ~m in diameter, with
walls smooth or roughened. The penicilli are one- or two-stage branched (one-stage
branched in those arising from aerial hyphae), 100 ~m long by 130 ~m in width.
Metulae divergent, 6.5-10.5 ~m (up to 13 ~m) by 2.5 ~m, with apices inflated in a
vesicle up to 4.0-4.5 ~m (up to 5.0 ~m) in diameter, in c1usters of 4.6-9 elements in
the verticil. Phialides bottle shaped, 8-1 O ~m by 2.0-2.5 ~m, terminating in a long,
tapered neck, in clusters of 5-8 (up to 11) elements in the verticil, seldom appearing
monoverticillate. Conidia are ovoid, 3.3-4.0 ~m by 2.5-3.3 ~m, echinulate, with
eonid ial chalns show lng conspl cuou s conneetlv es between them, at first
uncoloured, then developing deep olive shades. Sclerotia are black and submerged
in the substratum.
Colonies on grape must agar growing rapidly, attaining a diameter of 75 mm in 13
days; radiately wrinkled, with centre raised, velvety, azonate, with a leathery clcse
textured basal felt, heavily sporing, at first in olve-slate gray shades, with margin
copper yellow, developing deeper slate gray -brown shades (after 3 weeks) without
marginal copper yellow colours, with a narrow growing margin (1-2 mm) colour
less; colony reverse radially wrinkled, at first showing leather brown shades, then
(after 3 weeks). developing dirty violet-brown shades, blackening in central areas
(owing to the production of sclerotia). The sclerotia are abundantly produced,
either partially submerged or completely in the substratum. The remaining charac
teristics duplicate the above description .
Upon potato-dextrose agar the sclerotia are abundantly produced, leathery, at
first colourless, then becoming deep brown to black coloured, with walls consisting
of polyhedral cells, 9-15 .tm by 5-10 .tm in diameter, being produced either super
ficially or submerged in the substratum.'
(Translated from the Russian by Prof. L. Vilasl.
Co lon ies typically loose text ured lo lan at c , growing rapidly , slightly to
heavily sporing , in lilac , ochre or avc lla ncous (never grcen) shades.
Conidiopho res va rying gre a tly in len gth , arising from the su bs trat um or as
branches from asce nd ing hyphae , with walls smooth or finely roug hened .
Pen icilli typi cally divaricate , va riable, usu all y cons isting of terminal clusters
of d ivergent metulae, or a ppearing as sim ple structures when borne on short
branches fro m asc end in g stipes .
P hialid es typically termin at ing in a lon g, ta pered , narrow neck.
Conid ia gen erally strongly ellipt ical to lemon sha ped, com mo nly a ppea ring
apiculate , smooth walled or nearly so .
K ey to sp cci cs
Paga
1. Colonies not producing sclerotia or masses of thick-walled cells.
Colonies not showing green, gray -green, or blue-g reen colours with the
ripening of conidia. Colonies deeply velvety or lanate, developing lilac,
ochre, brown, or avellaneous shades .
P. Iilacinum series
a. Colonies tanate. developing Iilacinum shades, with reverse similarly
coloured.
P. lilacinum Thom
(Paecilomyces lilacinus Samson)
b. Colonies velvety, comparatively thin, not developing lilacinum
shades.
1'. Colonies spreading broadly upon most substrata , developing
pinkish buff to avellaneous shades.
P. humulivan Beyma
2' . Colonies growing very restrictedly upon Czapek's yeast extract
agar.
aa. Colon ies developing avellaneous shades upon malt extract agar.
P. argillaceum Stolk, Evans et Nilsson
bb. Colonies developing brown to ochraceous shades upon malt
extract agar.
P. cylindrosporum Smith
. .. . 228
.... 229
. . . . 231
... . 233
.. .. 236
SO. Penicill ium lilacinum Thom : fig. 80, colou r plate 80, and SE M piate
14 , (top: right).
11
O 10 O \J
0IJO o\:)
o
~ \J~
10 prn
, c~
~.~
'~ "
eR
~J/
;:/
Penicillium Ii/acinum Thom - Bull , Bur. Anim. Ind . U.S . Dep. Agric. 118: 73-75.
1910. See also Ch. Thom, 'The Penicillia', pp. 331-334. 1930.
Combinatlo nova
Paecylomyces Iilacinus (Thom) Samsom - Studies in Mycology. 6: 58-62. 1974.
Synonymies
? Penicillium amethystinum Wehmer sensu Biourge - in Biourge's Monographie,
La Cellule. 33: 221. 1923.
Graphium cicadicicola Spegazzini - An. Mus. Nac. Hist. Nat. Buenos Aires. 20:
476. 1911.
Penicillium (Scopulariopsisl rubellum Bainier sensu Biourge - in Biourge's
Monographie, La Cellule. 33: 221. 1923.
Spicaria violacea Petch - Trans. Brit. Mycol. Soco 16: 239. 1931. (non Abbott,
1926).
Spicaria rubidopurpurea Aoki - Bull. Ser. Exp. Stn Jpn. 10: 440. 1941.
stc r ilc aeria l h yph ac ; cvc n m a r gin surroundcrl by a Iringc 01' sub mc rgcd
hyph ac , cx tc ncliru; about 1 111111 bcyond thc ae ria l grow th; cx ucla tc and odo ur
lackin g: a m be r-co lourc d pi gm cnt diffusi ng inr o t.hc surro u nd ing aga r; colony
reverso (colou r plat e 80, b) in rc dd ish brown to ci n na rnon shadcs ; pcni cilli
csscntiall y as dc scrib cd above.
Colon ics upon M EA (colour platc 80 , C and c) attaining a d iamcter 01' 40
IlIlll in 14 days a t room te mperature (20-25C), cons isting of a loose-t extured
basal Ielt , ovc rlaid by a looscly wove n net work 01' floccose hyphae , len ding a
lan ate appea rance to th e colony surface; heavily sp oring th rough out in lilac
sha des; colon y surface not wrinkled, slightly raised in th e cent re, with thin
margin surrou nde d by a fringe 01' sub me rge d hyphae exte nding abo u t 2 mm
bcyond th e ae r ial growth; odo u r a nd cxudate lackin g; no coloured pigm ent
c1i ffusin g into th e surro u nd ing agar; colony reverse (colour plate 80 , e) in
sienna brown sha des; peni cilli essentially as described aboye .
The spec ies description is ce ntered up on th e typ e stra in CBS 284 .36 = CM !
27,83 0 = ATCC 10,11 4 = ATCC 1,11 23 = N R RL 895 = Q M 7,592 = IJ FM 5 116,
isolat cd from soil by C h . T hom in th e USA, a nd m any add itional cultures
isolat ed by the author from Span ish so ils. Amo n g th ese strain IJ FM 5 150,
isolat ed as a cultu re conta m ina n t in M ay 1978 , m ay be regarded as
rep rescntativ e.
o0
O
0o 0
0 0 11
0
~O O
00
0 00
00
"",Ve-"":.}
.
----==---
100 J.lm
..
1~
f..
from th e basal felt , sm ooth wall ed ; phialides bottle shaped , usually in clusters
of 2- 8 elem ents in th e verticil, mostIy 9-15 p.m by 2. 5-3 .0 uitv, terminating in
a com pa ra tively long, narrowed taper ed neck ; conid ia elliptica l to su bg lobose,
sm oo th walled , m ostIy 3 .0- 4 .0 p.m by 2. 8 -3 .5 p.m (fig. 8 1, B, a nd SEM plate
14, centre: left) ; conid ial chains loosely tangled (fig . 81, C) .
Colon ies upon C YA (col our piate 81 , R and b) attaining a diameter of 60
mm in 14 days at room temper ature (20 - 25 C) , consisting of a tou gh , close textured bas al feIt , strong ly furrowed in a radial pattern , rais ed gra dually
tow ar ds th e ce ntral arc as, wh ere th e m yceli al felt is ofte n cra cked on upper
surface s of folds, and strongly and irregula rly fold ed ; heavily spo ring
throu gh out in cinna mo n shades , partially obsc u re d in ce ntral a nd subcentral
arcas by a thin and dense ove rg ro wth of close ly int erl acin g trailin g white
hyphae; eve n thin m argin largely surro u nde d by a fringe of su bme rge d hyphae
exte ndi ng more tha n 5 mm beyond th e ae rial gro wth; ex udate and odo u r
lacking; coloured pigment diffusing int o th e surrou nd ing agar not produced ;
colony reverse (colour plat e 8 1, b) stro ng ly pulling away th e agar underneath,
developing cin namo n shades ; peni cilli esse ntially as described aboye.
"':/i Ov
",
'V/'
"
:;;
r,
O~~
o,
' 0
!!:
0 0
{/
\)("\OO
'. BJJ=
'. 0
~: .
1::.:::
00'
: -,
'i,.
\:'' t
.
;
~m
r ,
~; l
"~~
~ ~ lC
L.-...-l
100
jrrn
eRo
e,
Penicillium orgillocoum Stolk, Evans e t NlIsson - Trans. Brlt. Mycol. Soco 53:
307-310. 1969.
Synonym
Geosmithia argillacea (Stolk, Evans et Nilsson) Pitt - Can. J. Bot. 57: 2021-2030.
1979.
The author's original diagnosis follows, since this adequately presents the species
in question:
'Colonies upon Czapek's agar attaining a diameter of 4-4.5 cm within 2 weeks at
30C; with surface growth almost velvety; somewhat loose textured, consisting of a
very thin network of hyphae either above or below the agar surface from which
conidial structures arise, in central areas overlaid by a thin overgrowth of loosely
interwoven hyphae bearing also penicilli; azonate; with margin irregularly outlined,
feathery; sporulating somewhat irregularly, showing brownish colours which range
from chamois and cinnamon-buff to avellaneous (Ridgway, PI. 30, 29, 40); exudate
lacking, odour reminiscent of pine resin; reverse of colonies uncoloured at first,
becoming yellowish or pinkish with age.
Colonies on malt extract agar spreading broadly, attaining a diameter of 6-6.5 cm
within 2 weeks at 30C, in texture and colour as on Czapek's agar but sporulating
more abundantly and with margin even , circular in outline; reverse uncoloured to
avellaneous.
Vegetative hyphae hyaline, smooth walled, 1.5-3 .tm in diameter. Conidiophores
arising either directly from the substratum or as branches from aerial hyphae,
varying greatly in size, ranging from 60 to 400 .tm in length by 2-3 (4) .tm in diameter, hyaline, septate, with walls usually roughened, but occasionally smooth
walled. Penicilli biverticillate-asymmetric, varying in complexity , mostly consisting
of somewhat appressed verticils of two to five metulae, bearing c1usters of
phialides, but occasionally developing larger penicilli showing one or two rami in
addition to the main axis . Monoverticillate heads are also presento Rami and
metulae, as well as metulae and phialides, may occur in the same verticil. AII
elements of the penicillus are hyaline, usually rough walled, but occasionally
smooth walled . Apices of conidiophores in monoverticillate heads are mostly somewhat inflated . Rami 12-35 by 1.5-3 .tm . Metulae 12-25 .tm by 1.5-3 .tm enlarged at
the apex to 4 .tm in diameter. Phialides 9-14 .tm by 1.5-2 .tm, typically occurring in
dense c1usters up to 10 in number, but in reduced smooth-walled penicilli mostly
few in number; consisting of a narrow cylindrical base, tapering more or less
abruptly to a narrowed conidium-bearing tube (neck), about 1-2 .tm by 1 .tm.
Conidia (2.5) 3-4 (4.5) .tm by 1.2-2 .tm, varying from cylindrical to ellipsoid and
ovoid, hyaline, smooth walled. Conidial chains tangled or adhering in loose , twisted
columns.
P. argillaceum is thermotolerant, having its minimum temperature at about 15C,
It9 optlmum at about 36C and ita maxlmum at about 6O oC . At high temperatures
(40-S0 0Cl the cultures are predominantly floccose, sporulating only poorly.'
O ur not es follow :
Colon ies upon CA (colour plat e , 82 , A and a) attaining a diamet er of 30- 35
mm in 14 days at room temperature (20- 25C), consisting of an extremely
loose-textured basal fe1t, mostly submerged, from which arise very sparingly
conid ial str uc ture s, lending a powdery appe a ran ce to th e colony, and a
cinna mo n coloured surface ; exu da te lacking; odour faint, aromatic; colony
rcverse (colour piate 82, a) colou rle ss; penicilli essentially as originally
dcscribed, with th e conid iopho re s arising from th e basal fe1t and from
uniculose hyphae (fig . 82 , A), strongly verrucose including th e phialides;
conid ia mostly cylindrical , 3 .5-5 p-m by 1.2-2 .0 p-m in diamet er, smooth
wall ed (fig . 82, B, and SEM plate 14, ce ntre : right); conidial ch a in s loose1y
tangled (fig . 82 , C) .
Colonies upon CYA (colour piat e 82 , B and b) growing restrictedly ,
atta ini ng a diameter of 25 mm in 14 days at room temperature (20-25 C) ,
consisting of a close-textured bas al felt overlaid by a white mycelial overgrowth
consisting of a closely interwoven network of ae rial hyphae influencing th e
colony appearance ; colony surface appea ring sublanate , slightly raised in the
centre; very poorly sporing, remaining persistently white ; exudate lacking;
odour faint , indefinite; no coloure d pigment diffusing into the surrounding
agar; colony rev ers e (colour piate 82 , b) colourless with cinnamon sh ades
showing through; penicilli essen tially as described aboye .
Colonies upon MEA (colour plate 82, C and e) growing more rapidly, attaining a di ameter of 40 mm in 14 days at room temperature (20-25 C) , consisting of a dense basal fe1t overlaid by a close1y interwoven network of floccose
ae rial hyphae , lending a lanate appearance to th e colony ; m argin irregularly
di ssected , surrounded by a fringe of submerged hyphae ext ending about 3 mm
beyond th e aerial growth; he avily sporing throughout in ave llaneou s shades
(Sguy, PI. XVII, 249); not furrowed , with tufts of white floccose a erial
hyphae in cen tral areas ; exudate lacking; odour faint, a rom atic ; no coloured
pigment diffusing into the surrounding agar; colony reverse (colour plate 82 , e)
in yellow to ochraceous shades; penicilli essentially as described aboye .
Penicillium argillaceum is be1ieved to be close1y re1ated to P. cylindrosporum
Smith, on account of th e av ellaneous to ochraceous colours of th e colonies, the
morphological details of its penicilli and the shape of th e conidia . The two
species, however, differ in th eir phialides, which are conspicuously roughened
in P. argillaceum and smooth wall ed in P. cylindrosporum , Moreov er, th e two
species ca n easily be distinguished from one another by their cultu ral aspe cto
Colonies upon MEA of P. cylindrosporum have a very dark gr eenish, almost
black rev erse (colour plate 83, e), while this pigment is lacking in P. argillaceum .
<::::J
OOO '::
c-..... B~ e'!
"V
c::::J""
O~
~o IJ
Cl ~
'~1-::,,~
~;j (t~
JI<
y
{-
~
L..----l
100J.lm
Penicillium cy/indrosporum Smith - Trans. Brit. Mycol. Soco 40: 483-484. 1957. See
also M. M. Kulik, 'A Compilation of Descriptions of New Penicil/ium species' .
Agric. Handbook No. 351. Agric. Res. Ser. U.S. Dep. Agric. p. 34. 1968.
Synonym
Geosmhie cvlindrospore (Smith) Pitt - Can. J . Bot. 57: 2021-2030. 1979.
Smith's original diagnosis follows:
' Colonias on Czapek agar thin velvety, inclined to be patchy, dull avellaneous,
with reverse uncoloured; on malt agar growing fairly well , velvety, dull avellaneous
wth sordid yellow margin and with reverse dark dirty greenish brown; on potato
ogar similar except that the reverse is somewhat more greenish; stated by Mrs J.
Robson (frorn whom the original culture was received) that green perspiration drops
ore produced in old cultures, but these have not been observed in subcultures;
penicillus sometimes with a more or less divergent cluster of metulae (the P.
citrinum tvpe) . but more often irregular, with a branch or branches from lower
nodes, bearing phialides alone or metulae and phialides mixed in the same verticil ,
somet imes showing the same mixed verticils in the terminal part of the structure;
eonidiophores long and sinuous, mostly arising from submerged mycelium, rough ,
2.5-4 .tm in diameter; rarni, when present, rough, 16-25 .tm by 2.5-4 .tm; metulae
usually 12-20 .tm long, but shorter, 9-10 .tm, in the more complieated heads, and
2-4 .tm in diameter; phialides almost cylindrical , tapering abruptly at the tip, very
regular in length, 11 .tm by 2.5-3 .tm ; eonidia usually cylindrical (hence the specific
epit het ), 4.2-5.5 .tm by 1.8-2.2 .tm, mostly 5 by 2 .tm, but oecasionally ovate to
ellipsoidal, 3.2-3.5 .tm by 2.2-2.5 .tm; eonidial chains divergent then tangled, or
sometimes in several loose, twisted columnar rnasses.'
room tcmper at u rc (20- 25C), typically vclv cty, pla no, with m argin il'rcgularly outlincd surrounded by l IHU 'I' OW Irin ge 01' sub me rged dark brown
hyphac ex ten d ing about 1 mm bcy ond th e acrial gro wth; hcavily sporing
throughout in dark avellaneous to ochraccou s shadcs; brown-coloured pigment
di ffu sing into th e surrounding ag a r ; colony rev erse (colour platc 83 , e) in d ark
green ish brown , alm ost black shades; penicilli csse n tia lly as originally
des cribed , with th e stipes 01' th e con id iop hores co nsp icuously warty (fig . 83, A)
a n d metulae usu all y smooth walled ; phi alides m ay range frorn 11 to 15 Jlm in
length by 2.5 - 3 .5 Jlm in di amet er.
The species descr iption is ce n te red upon th e typ e strain C BS 275.58 = C M I
71 ,62 3 = AT CC 18 ,223 = I FO 8 , 110 = Q M 7, 524 = IJF M 704 3, isolat ed as a
cu lture contaminant by Mrs J. Robson , 0 1' the At omic Energy Es ta blishmen t,
Harwell , UK , in 1957.
This species is to be elas sified in th e Asymmetrica -divaricat a su b-sec tion,
within th e Penicillium lilacinum series on accou n t 0 1' th e tot al la ck al' green 0 1'
blue colou rs in its colo n ies .
Key to spccies
Page
.. . . 238
. .. . 243
. . . . 254
. . . . 240
.. .. 246
... . 248
... . 250
The Penicillium j anthinellum series erec ted by Ch . Thom (1 930) to accomo datc
an unusualIy variable, individuall y and collectiv eIy, group of Penicillia with
colony texture rangin g from com pa ra tiveIy thin, veIvety, loose textured to
lanat e . They a re cha rac terize d by th eir m or e or less fast gro wing , more or lcss
bri ghtl y coloured colonies, by th eir irregular and stro ngly divergent penicilli ,
and particul arl y by thei r phial ides characteristicalIy tapering to a long and
narrow neck . Eight spec ies a re recognized in th e cu rre nt stud y , as follows:
Penicillium daleae Zal eski , P. asturianum Ramrez et M artnez , P. Janthinellum
Biourge , P. simp licissimu m (O ude mans) Thom , P. ochro-chloron Biourge , P.
piscarium W estling, P. aragonense Ramrez et Martnez , and P. miczinskii
Zaleskii. P. godleswski i Zaleski , characterize d by colon ies gene ra lIy more or less
funi cul ose , included by R aper a nd T ho m (1949) in thi s seri es, has be cn
exclude d from here to design a te th e newl y erec ted Penicillium godlewskii seri es .
Penicillium daleae and P . as tu r ia n um are distinguished particularly by th c
spira l ban ding of conid ia . Penicillium janthinellum is by far the mo st abundant
a nd most va riable. When first isolated , strains are com monly brightly
coloured, but u sualI y becom e less colou rful with con tin ue d labor atory
cultivation . Penicill ium simp licissimum is usualIy a heavil y spo ri ng form upon alI
su bstrata a nd less colou re d, eithe r in th e vegetative hyphae or in th e colony
rever se. Penicillium ochro-chloron is not eworthy for its conspicuo u sly warty
con id iopho re s and its tolerance to coppe r and high acidities. Penicillium
piscarium is a com pa ra tiveIy loose textured , lanat e form with su bg lobo se and
stro ngly echi n ulate con id ia . Penicillium aragonense, is a close textured, veIvety
form , cha racte rized by its com pa ra tively large , eIliptical, smooth walIed
conid ia. Penicillium miczynskii is di stinguished by its colonies at first white ,
velve ty, becoming blue-gr een with th e ripe n ing of conid ia, with yelIow
vegeta tive hyphae abu ndantly produced.
L..-.........J
100}Jm
13: 94-95. 1918; also Raper and Thom, 'A Manual of the Penicillia', pp. 299-303.
1949.
Synonymies
Penicillium echinulonalgiovense Abe - J . Gen. Appl. Microbiol., Tokyo. 2: 81.
1956.
Penicillium guttulosum Gilman et Abbott - lowa State Col. J. Sci. 1: 298. 1927.
Penicillium glauco-roseum Demelius - Verhand. Zool. Bot. Gesellsch. Wien . 72:
72 . 1923.
Penicillium meleagrinum, varo viridiflavum Abe - J. Gen. Appl. Microbiol. Tokyo.
2: 92. 1956.
Penicillium rivoliZaleski - Bull. Acad. Polonaise Sci. Math. Nat. Sr. B. pp. 471473. 1927.
oloni es upon C A (eolour plat o B4, A and a) attu in ing 11 di amct cr 01' :~ 0 -:~5
mm in sorn e stra ins, while 60 - 70 mm in o thc rs, in 14 da ys al room
ternperature (2 - 25 C) , cons isting of a lou gh , closc-texturcd basal 1'c11
overlaid by a closely interwoven nctwork ol' fine hyphae lending a dclicat ely
lanate ap peara nce to th e colony su rface, whi ch is unevenl y tuftcd, abruptly
rai sed , or in sorne strains somewhat fu n iculose, irregul arl y wrinkled in th e
centre and radially furrowed in m arginal areas ; with an cve n margin
surrounded by a fringe 01' submerged hyphae extending about 1 mm beyond
th e ae rial gro wth ; at first white , but in recent isolat es becomin g va rious ly
colou re d from th e tardily and irregul arly sporing areas, mostly in pale gray
(Sgu y , PI. XXIX, 430) to glauc us gray (Sguy , PI. XXX, 444 ), and th e
sim ultaneous shadi ng 01' non-sporulating areas to dull bu 1'1', ora nge -red , or in
sorne strains purple vinaceous shad es; azonate to broadl y zonate; exudate
lacking or limited in a m ou nt; odo ur strong , typi cally suggesting a roma 01' red
beet; no colou re d pigment diffusing into the surroundi ng a gar; colony reverse
(colou r plat e 84 , a) colou rless in old isolat es a l'ter m an y tr ansfers, but usually
in bright shades in recent isolates, com monly ye llow-g ree n to orange at first ,
changing to ora nge -red, m aroon or purpl e-red colou rs; penicilli typi cally
asy m me tric, strong ly di varicat e (fig. 84, A), abu ndantly produccd in sorne
strains , less in othe rs, sometimes eve nly distributed throu gh out th e colony bu t
usuall y m ore abundant in submarginal areas, borne apically on asce nd ing
con id iopho res up to 500 tm in len gth by 2. 0 -3. 0 tm in diamet er , with wall s
smooth or fin e1 y rou gh en ed , or on sho rt branc hes from ae rial hyphae , m ostl y
10- 50 tut: by 2.0 -2.8 tut , va rying greatly in com plexity, rangin g from simple
verticils 01' phialides (appearing monov erticillate) to verticils 01' m etulae 01'
unequal length , or ve rticils of both m etulae and phialides ; branch es va ria ble,
rangin g from 10 to 25 tm by 2. 0- 2 .3 tu ; metulae , m ostl y 10-1 5 uu: or
lon ger, up to 20 tm by 2 .0-2.2 tun, with a pices slightly enla rged in a vesicle up
to 3.0 tm in diameter; phialides divergent , va ria ble in dimensions, ranging
from 9 to 20 tm by 2.5-4.0 tm in the sim ple pen icilli to 7-11 tm by 2. 5-3 .0
tm in th e biverticillate asymmetrical str uc tu res , abru ptly tapering in a long,
narrowed ne ck , characte ristic 01' the series; con id ia strongly ellipt ical wh en
formed , u su all y rem aining so , but in sorne strains becoming ovate to
su bglobose with ends 01't en apicul at e and with walls more or less rou gh en ed
(fig. 84 , B , a nd SEM plat e 14, bottom : ri ght) , m ostly 3- 4 tm by 2.8 -3.2 tm
in di amet er; conid ial chains 100se1y divergent (fig. 84, C ).
Colonies upon C YA (colour plat e 84, B and b ) attaining a di ameter 01' 50
mm in 14 days at roo m temperatu re (20 -25 C), cons isting 01' a tou gh , closetextured ba sal felt , overl aid by a closely int erwoven network of fine aerial
hyphae lending a su bla nate a ppearance to th e colo ny surface, dee ply ra d ia tely
furrowed in su b marg ina l and m argin al arcas, with cent re rai sed and depressed
in a crateriform pattern ; zonate; thin , eve n m argin with a fringe 01' su bmerged
hyphae cx tc nd ing ab out 2 mm bcy ond th e Heria) gro wth ; heavily sporin g
thr ou ghout in bro wn ish {!'aY shade s, partially obscurcd by the dense network
1I1' fin e acrial hyphac d cli cat cly tintcd in lilac shadcs , cspcc ially in cen tra l a nd
su hcc n tral arcas; ycl low-co lo urcd pigmcu t di llusing into the surrounding agar;
odour strong, typically suggest ing red bce ts; ex udate lacking; colo ny reve rso
(cnlour plate 84 , b) in violet to ora nge sh ade s ; pcnicil1i essential1y as descri bed
ubove .
C olon ies u po n MEA (colour plate 84, C and e) attaining a diameter 01' 60
1I11ll in 14 days at room temperature (20-25C), consisting 01' a th in felt but 01'
a tcx ture looser than upon CA and CYA, con spicuousIy zonate, at first whit c,
(h cn becoming pale yellow; co lon y surface de ep ly la n a te , with thin margin
su rrou n ded by a wide fringe 01' submerged hyph ae , extending 10 mm 01' more
hcyond the aerial growth ; not furrowed, slightly convex; h eavily sporing in
g"l'a y shades ; exudate la cking; odour less pronounced but similar as des cribed
ah oye; n o colou red pigment diffusing into the surrounding agar; colony
rc ve rse (colour plate 84, c) in yellow -orange sh ades; penicilli com m only la r ger
Ib an upon CA and CYA but essen tially similar in pattern .
The sp ecies description is centered on strain CBS 340. 48 = C M I 40,238 =
A'I'CC 10,455 = NRRL 2,0 16 = QM 6 ,865 = IJFM 3863, isolated by K .B . Raper
rorn Ni caragua soil in 1945, a nd n umerous other strains isolated by us in
Spain .
T his species is probably the most abundant Penicillium found in soi ls, being
cosmopolitan in origin o It is al so us ually isola ted from decaying vegetation
undergoing th e final stages 01' de composition.
B5. Penicillium daleae Zaleski: fig. 85, colour plate 85, and SEM plate 15,
(top : left) .
Penici/lium daleae Zaleski - Bul!. Acad . Polonaise Sci. Math . Nat. Sr. B, pp. 495496. 1927. See also Ch. Thom , 'The Penicillia' , pp. 360-361. 1930; also Raper and
Thorn, 'A Manual of the Penicillia ', pp. 296-299. 1949.
Synonymy
Penici/lium krzemieniewskii Zaleski - Bul!. Acad. Polonaise Sci. Math. Nat. Sr.
B, pp. 490-492. 1927.
Zaleski's original diagnosis follows, since this adequately presented the species in
question when first isolated :
'Colonies in neutra l Raulin with 10% gelatin in Petri dishes, growing slowly,
attaining a diameter of 24-26 mm in 12 days, liquefying the gelatin tardily but
completely, velvety or somewhat subfloccose, zonate but indistinctly and in
marginal area, with t he whole central area broadly radiately fu rrowed , w ith centre
JI
....
",..,
ilii1>~f~ua;~~ , ~
'l J \~
100,..m
somewhat depressed and showing few colourless drops of exudate; white margin
2-3 mm wide; conidial areas at first developing blue-green shades (Klincksiek and
Valette's Code des Couleurs, Paris, 1908, No. 371, 372), becoming dark yellowgreen (Klincksiek & Valette's Code No. 273), and later dark orange-brown such as
(168, 164, 198, 139); colony reverse at first in orange yellow shades such as (171,
166, 157, to 133, 138), later becoming red-orange (84, 88, 92, 97); odour lacking or
faint; conidiophores 10-200 or 300 Jlm by 2-2.5 Jlm, with apices more or less
enlarged in a vesicle, commonly unbranched, occasionally with short branches,
flexuous, varying greatly in length, erect or ascending; penicilli mostly 10-20 .tm,
less frequently 25-30 Jlm or 40 Jlm long, with walls smooth; metulae 8, 10 Jlm-20 or
24 Jlm in length by 2.5-3.0 Jlm in diameter, in groups of 2 or 3, commonly unequal in
length and irregularly arranged, with apices commonly inflated; phialides about
9-10 Jlm by 2.5-3.0 Jlm, commonly in verticils of 3, 5-10 or 12 elernents, sornetimes occurring singly; conidia 2.5-4.0 Jlm by 2.5-3.0 Jlm, varying considerably in
size, coarsely echinulate, ovate elongated or subglobose .
Habitat: Strain isolated from pine forest soil near Poznan, Poland.'
The type st ru in 01' Penicillium daleae Za lcski exa m incd 1'01' th c pr cscnt study,
scnt by th c C en tra albureau voor Schimmclculturcs , Baa rn , Thc Nctherlands,
as stra in c ns 211 .28 , no longer produces colonies confo rm ing with Zaleskis
(1 927) dcscription , nor does it produ ce well -developcd penicilli on any su bstra tu rn tested . The conidia , how ev er, are entirely cha rac teristic of the species .
O u r not es follow :
Colonies upon CA (colour plat e 85, A and a) attaining a di amet er 01' 43 mm
in 14 da ys at room temperature , con sisting 01' a e1ose-t extured ba sal felt ,
uppea ring velvety 01' lanate owing to th e thin overgrowth 01' close ly interwoven
ac rial hyphae and ropes 01' hyphae ; ra d iately furrow ed , alm ost azonate, with
marginal are a becoming slightly gray colou re d with th e sparse devel opment 01'
ro nid ial area s; exuda te a nd odour lacking; no coloured pigm ent di ffusin g into
lil e su rro u nd ing agar ; colony reverse (colou r plate 85, a) colourless to pale
rrca rn ; penicilli very sim ple , consisting 01' single phialides 01' very small el ust er s
of phi alides (fig. 85, A) , 8-12 p,m by 2.0-2 .5 tun , te rm inating in an a bru ptly
narrowed neck; structures suggesting true peni cilli a re rare ; co nid ia are
cllip tical to su b -globo se, 3.0 - 3 .5 p,m by 2.5 -3. 0 p,m , coarse ly roughen ed , with
rou ghness a rrange d in spirally bands 01' bars (fig . 85, B, a nd SEM plat e 15,
rop : left) ; conid ial chains loosel y parallel (fig . 85, C) .
Colonies upon CYA (colour plate 85 B and b) attaining a di amet er 01' 50 mm
in 14 days at room temperature (20 -25C) , duplicating in texture a nd colony
a ppea ra nce those gro wn upon CA , but spo ring better in m arginal area s in gray
xha des (Sg uy , PI. XXXV , 522) ; thin , eve n margin su rrou nde d by a frin ge 01'
subrnerge d hyphae exte nd ing 2- 3 mm beyond th e ae rial gro wth; am be rr oloure d pigment diffusin g into th e surro u ndin g agar; exudate and odo u r
lacking; colony surface ove rlaid by a wh ite network 01' closely woven ae rial
hyph ae obsc u rin g greatly th e con id ial area s; lending a lan at e appearance to th e
colonies; colony revers e (colour pIat e 85, b) in brownish to orange shades;
pcnicilli esse ntially as described aboye.
Colon ies upon M EA (colour pIat e 85, C , a nd c) atta ining a di am eter al' 45
mm in 14 days at room temperature (20- 25 C ), sim ila r in texture a s described
ab oye , but not furrowed; heavil y sporing in brownish gr ay shade s (Sguy , PI.
XVI , 23 1); velv et y to lanat e , with thin m argin surrounded by a frin ge 01'
su bmerged hyphae exte nd ing 2- 3 mm beyond th e aeri al grow th ; pen icilli
cssentially a s described , mostly appearing sim ple; ex udate and odo u r lacking;
brown-coloured pigm ent di ffusing into th e su rro u nd ing agar; colony revers e
(colou r plat e 85, e) in ora nge shade s.
The species description is ce nte re d upon th e type strain CBS 211.28 = CMI
34.9 10 = ATcc l 0,435 = IF06,087 = NRRL2 ,025 = IJFM 5117 ,isolatedbyK.
Zaleski from a pine forest soil near Poznan, Poland in 1927 . (C olour pIat es 228
and 229 present two representative strains 01' P. daleae, isolat ed from an andosol
in the province 01' Navarra , Spain , 1976, by DI'. A .T. Martnez.)
lw.J.W.W l
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Synonym
Spicaria simplicissima Oudemans - Nederl. Kruidk. Arch. Ser. 3, 2: 763. 1903.
See also C.N. Jensen, Bull. Cornell Univ . Agric. Exp. Sta. 315: 493. 1912.
(SI: gUY, PI. XXXIII , 4112 , 4IU); exuda re limit cd lo abundaut , cry sta l clca r ,
rulou rlcs s; odo u r lackiu g ; no co lo urcc l pigm ent dilfusin g inr o th c su rro u nd ing
:I~a r ; colon y reve rso (colour plat o 1I6, a) in bul' shadc s with margin in ycllowol'ang"e colours ; pcnicilli typi call y asy rn mct r ic (fig . 86 , A) and divaricate ,
usually consisting 01' terminal clus te rs al' 2 -4 divergent metulae bearing
vc rt icils al' phialides (appcarin g esse ntially simple) , borne upon ascending
xtipos arisin g m ostly from th e substratum, al' from veg etative aerial hyphae
Ilpon sho rte r stipes; con id iophores with walls fin ely roughened , varying greatly
iu dimensions , rangin g from 200 to 800 11m al' more in length b y 2.5 -4 .0 11m in
d i.unc te r in larger structures, to very short, usuall y less th an 50 11m by 2.5 11m,
whc n arising as lat er al branch es frorn ve getative hyphae; metulae va riable,
Illoslly 12- 20 11m b y 2. 5- 3 .5 11m ; phialides in whorl s al' 3 - 10 elements in the
ve rticil , mostly 10-1 3 11m by 3 .0 -3.5 11m , abruptly tap ering to a consp icu ou s,
n.urowed , lon g neck ; con id ia a t fir st ellip tical , usuall y remaining so but
Irc que ntly appearing subglobose, mostly 3.0-4.0 11m in long axis , with walls
Iin cly roughened wh en observed under the light mi cr oscope (fig . 86 , B) but
with wall ornamentati on a p pear ing cere b rifor m when observe d under th e
SEM (plate 15 , top : right) ; con id ia l chains in long loosel y parallel colu m ns (li g .
11<;, C) .
Colon ies upon C YA (colour plate 86, B a n d b) a ttain ing a di amet er a l' 55
in 14 d ays at room temperature (20 -25 C) , sim ila r in texture to those
g-rown upon CA, but more d eeply su lca ted with numerou s radial furrow s, a nd
hcavily sporin g in blue-gr een to artemisia gree n shades, with the gr een colou r
prcd ominant (Sgu y , PI. XXVII , 403 , 40 4) ; thin, even margin surrounded by
a wide frin ge al' su b me rge d h yph ae exte nd ing about 4 mm beyond th e ae rial
g-rowth; ex u date limited , as crystal c1ear , uncoloured droplets ; odour lacking;
110 coloured pigment diffu sin g into th e su r rou n d ing agar; colony reverse
(colou r pl at e 86, b) in buff to cin namo n shades; penicilli essen tially as
dcscribed a bo ye, b u t with con id ia l chains tangled.
Colo n ies upon MEA (col our pIat e 86, C a n d c) attaining a diamet er al' 60
mm in 14 d a ys at ro om temperature (20- 25 C) , cons istin g a l' a looser textured
basal felt , plane , not furrowed , with thin m argin surrounded by a frin ge a l'
sub me rge d hyphae ext ending ab ou t 3 - 4 mm beyond th e ae r ial gro wth; heavily
spor ing throu gh ou t in blue- green shades (Sgu y, PI. XXXIII , 488); odour a nd
exudare lacking ; no coloured pigment di ffusing into th e su rro un d ing agar;
colony reverse (colour plat e 86 , c) colou rless with a green ca st showing
through ; penicilli essen tially as d escribed a boye, but with th e con id iopho re s
a rising primaril y from th e su bs tratu m, a nd with the conidial chains tangled.
The sp ecies description is ce n te red upon strain C B S 372.48 = C M I 39, 8 16 =
ATCC 10,495 = NRRL 902 = IF O 5,762 = QM 1,0 39 = IJFM 704 8 , isolat ed in 1940
Irom a flannel b a g by Mrs E .M . Laughton , Cape Provin ce, South Africa, and
add ition al cu ltures isolated from soil in Spain by th e author al' th e current
stu dy.
IIIIn
B7 . Penicillium ochro -chloron Biou rge : fig. 117 , colour plate B7 , and SE M
platc 15 (centre: len ).
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Synonymies
Penicillium cuprophilum Sato - J . Agric. Chem. Soco Jpn. 15: 359-369. 1939; and
Bull . Agric. Chem. Soco Jpn . 15: 77. 1939.
Penicillium biforme Thom varo vitriolum Sato - J. Agr ic. Chem. Soc oJpn . 15:
359-369. 1939; and Bul!. Agric. Chem. Soco Jpn . 15: 76-77. 1939.
cou ony owing lo thc closely int erw ovcn , fine , veget ativo, aerial hyphae
overlying th e co lony su rfac e , partially obs curing the otherwise heavil y sporing
a rcas with radial tufts uf white f1occose hyphae ; colony surface radially
furrowed and raised in central arca, azonate 0 1' in some cases be coming zonate
with the development 01' conidial structures ; thin, even margin surrounded by
a frin ge 01' submerged hyphae exte nding about 5 mm beyond the aerial growth;
hcavily sporing throughout in deep olive gre en shades (Sguy, PI. XXIX,
11 ~ 6) ; exudat e abundantly produced in some stra in s, not in others; odour
lacking ; no coloured pigm ent diffusing into the surrounding ag ar; colony
rcverse (colour plate 87, a) in buff to flesh shade s, with margin developing
or an ge colou rs; penicilli usually more con centrated in m argin al areas , variable
in size and in com plexity, strongly divaricate (fig. 87, A); conidiophores
va riable in length, 01'ten a risin g as branch es from aerial hyphae a nd rarely
cxceeding 100 p.m in length by 2.0-2 .5 p.m in di amet er, less com monly arising
lrorn th e substratum , up to 300 - 500 p.m in length by 2.0-2 .5 01' 3. 0 p.m in
c1iamet er , with walls conspicu ou sly warty ; penicilli appearing variously sim ple,
irr egul arly ramified, 01' occasionally with fairly well defined ve rtic ils 0 1' metulae
IIr br an ches, variabl e in dimensions, ran ging from 10 to 20 p.m by 2 .5 - 3 .0 usn ,
with walls com mo nly warty; phialides in whorls 01' 3-1 O elements in th e
vcrticil, divergent , mo stly 10-12 p.m by 2.5- 3.0 uu, abruptly tapering to a
cons picu ously long, narrowed neck; conidia elliptical with on e end com mo nly
upicula te, usuall y 3.0 - 4 .0 p.m by 2.0- 3.0 p.m in diamet er, with wall s sm ooth
or deli cately roughen ed (fig . 87, B, a nd SEM plate 15, centre: left) .
Co lonies upon CYA (colour plate 87, B and b) att a ining a diameter 0 1' 60
mm in 14 days at room temperature (20 - 25 C ) , co nsisting 0 1' a sim ila rly
(cxtured basal feIt as described aboye , deepl y velvety to lanate, radiately
sulcated with numerous fu rro ws; eve n margin surrounded by a wid e fringe
cxtend ing about 5 mm beyond th e ae rial growth ; abu ndantly spo ring
through out in gray-green shad es (Sguy, PI. XXIX , 427) , partially obs cured ,
cspecially in m arginal areas by th e floccose overgrowth of closely interw oven
ae rial hyphae ; exudate abundantly produced as crys tal-clear, uncoloured
drops; odo ur lacking ; no coloured pigment diffusin g into th e surrounding
agar; colony revers e (colour plate 87, b) in dark cinnamo n shad es with margin
tinted in orange colours; penicilli essentia lly as described ab oye , with conidial
cha ins loosely parallel to tangled (fig . 87, C ) .
Colonies upon M EA (colour plat e 87 , C and e) attaining a di amet er of 55
m m in 14 days at room temperature (20 - 25C) , con sisting 01' a looser textured
basal feIt , velvety, plane, not furrowed , with thin , eve n margin su rro u nde d by
a frin ge of sub me rge d hyphae exte nd ing abo u t 1- 2 mm bcyond th e ae rial
growth; heavil y sporing throughout in gray-green shades (Sgu y , PI. XXIX,
429); colony surface ove rla id by a thin netw ork of aeri al hyphae and thin ropes
of hyphae; exu date and odour lacking; no coloured pigm ent diffu sing into th e
Uw.J.=.J
10~m
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.:
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100~m
CK.
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Penicillium piscarium Westling - Ark. Bot. 11: 54. 1911. See also Biourge's
Monographie, La Cellule. 33: 190-191. 1923; also Ch. Thom, 'The Penicillia', pp.
487-488. 1930; and Raper and Thom, 'A Manual of the Penicillia', pp. 308-309.
1949.
basal fclt , ov c rlaid by a dee ply Iloccosc ove rgrow th 01' interwo ve n aerial, whi tc
hyph ae , len di ng a lanat e a ppcara ncc to the colony surfacc; spo ring in graygree n shades (S guy, PI. XXIX, 428) , bu t with conid ial arcas mostly obs cu red
by th e floccose white overgrowth , exce pt in th e ce ntra l a rea; exudate and odo ur
Iacki ng ; no coloured pi gm en t di ffusi ng int o th e su rraund ing a gar ; colony
reverse in eream sha des (colour plat e 88, e) ; eonid iopho res u su ally longer th a n
on CA, rangin g from 50 to 100 .tm in len gth wh en borne as branehes and with
tr a ilin g hyphae mor e ofte n terminating in pen ieilli ; penicilli as described
aboye .
The spee ies deseription is een tere d up on th e typ e st ra in CB S 362 .48 = CMl
40 ,032 = ATCC 104,926 = NRRL 1,075 = IFO 8, 111 = V KM F-1 ,82 3 = IJFM
7056, and strain C BS 220 .30 = N R RL 2132 (both presu m abIy de seendin~ from
W estlin g' s type), isoIat ed from eod Iiver oiI by W estling in 1911. OnIy on e
add itionaI strain approx imating Penicillium p iscarium was isolat ed by P .B.
M arsh fro m a pieee of u n treated cotto n due k at Be1tsville , M aryIand , USA , as
NR R L 2022 in 1949.
I
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100 prn
fR
n;c;//;um miczvnskli Zaleski - Bull. Acad . Polonaise Sci. Math. Nat. Sr. B, pp.
482-484. 1927. See also Ch. Thorn , 'The Penicillia', pp. 488-489.1930; also Raper
and Thom, 'A Manual of the Penicillia', pp. 309-312. 1949.
Synonymies
Peniclllium sulfureum Sopp - in Ch. Thom, 'The Penicillia', p. 451 . 1930.
Penictium menqini Duch et Heim - Trav. Crypt. ddis a L. Mangin . Mus. Nat.
Hist. Nat., Paris, pp. 431-458. 1931.
mm in 14 d ay s a l 1'00 m tcm pcratu re (20 -2:>0) , co ns isiing 0 1' a dceply velv ety
felt , with thin rnar gin su rro u nde d by a wid c Irin gc 01' subme rgc d hyph ae
exte nd ing abo u t 5 mm beyond the ae rial gro wt h; colony surfacc plane
somewha t radi ately fur rowed in the centre, a ppear ing slightly floccosc owing to
th e loose-t extured floccose ove rgrowth; heavily sporing throu ghout in bluegreen shade s (Sguy, PI. X X XIII , 482) ; ex udate and odo ur lacking; no
colou red pigment diffusin g into th e su rro u nd ing agar; colony rever se (co lou r
plat e 89, c) in cream shades; penicilli esse nt ially as described aboye, with
conid ial cha ins loosely parallel to tan gled .
T he species description is centered up on th e typ e stra in C BS 220 .28 = CMI
40 ,0 30 = ATCC 10470 = NRRL 1,077 = IFa 7,730 = Q M 1,957 = IJ FM 5125,
isolat ed by K . Zaleski , Pol and , 1927 , from coni fer forest soil.
The presen ce 01' aggregates 01' inflated , heavy-walled cells in strai ns,
othe rw ise closely approx imating Penicillium miczynskii, suggests a relatio ns hip 01'
thi s spec ies to P. soppii in th e P. raistrickii seri es, hen ce its inclu sion in th e key to
th e Penicillium raistrickii series.
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100J.lm
th rough out in de cp blu e-grccn shadcs (S guy , PI. XXX, 4106); vcry thi n
margin surroundcd by a wid c frin ge 01' sub mc rged hyph nc cxtc nd ing abo ut 1mm beyond th e ae rial grow th; exuda tc and odo ur lackin g ; pale a m bcreoloured pigme n t diffu sin g in to the surrounding agar; colony rcverse (colou r
plat e 90, e) in pale yellow to ora nge sha des ; peni eilli cssc ntially as described
aboye , but with eonidiopho res mostly arising prim aril y from the su bstra tum ;
eonid ial ehains in fairly lon g eompae t eolu m ns .
T he spe eies deseription is based on th e typ e stra in IJ F M 3871 = ATCC 42,2 26,
isolated by th e au thor from th e a ir 01' M adrid , 1977 . It is at present kn own only
from the typ e strain .
i'
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~
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.'
velvcty , co nsisting 01' a close-textu rcd basal fclt , com pa ra tivcly thin, with
white , cvcn rnargin surrounded by a Irin ge 01' dccply sub mc rged hyph ac
cxtending 2 -3 mm beyond th e acrial growth; colony su rface sulca tcd hy
shullow radial furrows, moderat ely raiscd towards th e cent re ; sporing
nbundantly throughout in pal e gray-green shades (Sguy , PI. XXIX, 429) lo
pule brownish colours (Sguy , PI. XXIX , 427); exu date a nd od our lacking;
nmbc r-colou red pigment diffusing into the surrounding agar; colony rcvcrsc
(co lou r pIate 91, a) in cream to pea ch shades; penicilli , both strictl y sim ple and
1I1so typically asymmetric and divaricate, with con id iophore s arising primarily
lrom th e substratum and from trailing vegetative hyphae (fig. 91, A), smooth
walled , usually short , less than 100 Lm in length by 2.5- 3 .5 Lm in diameter,
with apices enlarged in a vesicle up to 5 Lm in diameter ; metulae in verticils 01'
" - 3 e1em ents 01' unequal len gth in th e verticil , mostly 18-45 Lm by 2.5- 3 .0
. /un ; phialides comparatively large , cylindrical , abruptIy tapered to a lon g,
narrowed neck, mostly 13-18 Lm by 2.8-4.0 tui, consisting ofwhorls 01' 3 - 8
cleme nts in the verticil, divergent; conidia usually elliptical to subglobo se ,
com paratively large, with walls smooth 0 1' nearly so (fig . 91 , B, and SEM plate
1(>, top: left) , mo stly 4.0-6.0 Lm by 3.2-5 .0 Lm in diameter, forming chains
with conspicuou s connec tives betw een th em ; con idial cha ins at first div ergent,
hecoming tangled in age (fig . 9 1, C) .
Colonies upon CYA (colour plate 91 , B and b) spread in g broadl y, attaining
a diameter 01' 55 mm in 14 days at room temperature (20-25C) , consisting 01'
a close-textu red bas al feIt, velv ety in appearance , deepl y radiatel y furrowed
with centre irregularly folded and raised ; white margin plane, with a fringe 01'
submerged hyphae extend ing about 1 mm beyond th e ae rial growth ; heavil y
sporing in blue-green shades (Sgu y, PI. XXX, 443); colony surface overla id
by a thin, close -textured network 01' fine , aeri al , white hyphae , partially
obscuring, especiall y in central and subcentral areas , th e conidial structu re s;
exudate and odour lacking; arnber-colou red pigment diffu sing into the
su rrou ndin g a ga r ; colony revers e (colour piate 91, b) in cinnamon to
ochrace ou s shades ; penicilli essentially as described aboye.
Colonies upon MEA (colour plat e 91 , C and e) attaining a diameter 01' 50
mm in 14 days at room temperature , quite sim ila r in texture to th ose gr own on
CA, consistin g 01' a thin , close-textured basal felt , showing some zon ation ; thin
margin su rrou nde d by a wid e fringe 01' sub me rge d hyphae , extend ing more
th an 10 mm b eyond th e aerial growth; colony surface deepl y radiately
furrowed , slightI y raised towards the centre; sporing abundantly in light bluegreen shades (Sguy , PI. XXX , 444) to pale brownish colou rs (Seguy , XLVII ,
694), depending on th e rip eness 01' con idia ; exu date a nd odo u r lacking ; colony
reverse in crea m to or ange shades (colour plate 91 , e); pale am be r-co lou red
pigm ent diffusing into the su rrou nding ag ar; penicilli esse ntially as described
aboye.
DOV .
Coloniae plus minus restrictae crescunt. velutinae, colore virido, griseo-virido, vel
coeruleo-virido ; penicilli divaricati, cum catenis conidiarum diversis, plus minus
compactis columnis formantur, cum phialidibus sine conspicuo collulo; conidia
exigua, non plus ultra 3 ]Lm in diametro; reverso fulgido coloratur.
Typus : Penicillium godlewskii Zaleski.
C olonies showing sorne sha des al' gr een , gray-gree n, 0 1' blue-gr een with th e
rip ening al' con id ia. Penicilli with the di varicat e charac te r well marked , with
conid ial cha ins divergent , usu ally tendin g to form m or e 01' less com pact
colu m ns . C olonies gro wing m ore 0 1' less restrictedl y , a t lea st on sorne
substrata . M etulae usually aggre ga ted into true ve rticils; phialides not
abruptly tapered to a long , narrow ed neck ; conid ia com pa ratively small,
u sually not excee d ing 3 p.rn in di amet er. C olon y reverse brightl y colou re d.
K ey to species
Paga
11. Colonies upon malt extract agar growing more rapidly, attaining a diameter exceeding 40 mm in 14 days.
A . Colonias upon malt extract agar conspicuously lanate.
1. Colony reverse developing bright orange -red shades upon malt
extract agar; similarly coloured pigment diffusing into the surroun ding agar.
P. harmonense Baghdadi .. .. 271
2. Colony reverse not as above.
P. sizovae Baghdadi . . . . 273
B. Colonies upon malt extract agar typically velvety.
1. Conidiophore walls smooth; colony reverse showing deep green
colours upon malt extract agar.
P. citreo-virens Abe ex Ramrez . .. . 279
2. Conidiophore walls conspicuously warty; colony reverse showing
typical strawberry red colour.
a. Colonies usually developi ng mouse gray shades with the ripening
of conidia.
P. novae-caledoniae Smith . . . . 275
b. Colonies remaining persistently white.
P. novae-caledoniae Smith, varo album Ramrez et Martnez ... . 278
l.l..J.J..WwJ
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fl?.
Synonym
Penicillium intricatum Thom - Bull. Bur. Anim. Ind. U.S. Dep. Agric. 118: 75-76.
1910.
Colon ies upon C A (colour pla te 92, A and a ) growing restri ctedly , attaining
a d ia meter of2 0 mm in 14 days at room te m perature (20 -25 C), cons isting of
a thin , to ugh, close -tex tured basal felt , developing usu all y wet , sodde n, mo re
or less bristly to funiculose colonies, characterist ica lly producin g a limited cro p
of con idia, rem a in in g persistently wh ite in sorne strains but in others
developing abu ndant conid ial str uc tu res in m arginal a re as , in ligh t blue
green shades (Sg uy , PI. XXX , 445); with m argin plane an d ce ntral and
subcentral a reas strong ly ra diately fu rrowed, with ce ntre folde d in a
crateriform patte rn ; odour indefini te or lackin g ; ex udate limi ted in amo u nt, as
colou rless d ro plet s enmes hed in the m ycelial felt; no coloured pigment
d iffusing in to the surro u nd ing agar; colon y reverse (colour plat e 92, a)
yellow ish; penicilli varia ble in size and pattern bu t typica lly d ivaricat e (fig. 92,
A); conidiop hores va riable in size and origi n, rangin g from 50 to 300 p.m in
"\)1
lcn gth by 1.9 - 2.4 Jtlll , ar ising eithc r di rcctly lrom th o su bs tratu m 01" as shorl
hrun chcs frorn ae rial hyphac , slighlly cn la rgcd a l th e apcx, with wall s smooth;
pcn icilli cons isti ng of singl e ve rticils 01' phial ides (a p pearing rnon overticilIat e) or
vert icils 01' m ctulae of unequal len gth , usu all y 2 - 4 eIeme nts in th e ve rticil,
nbo ut 8 -1 4 Jtm by 1.5- 2.8 u in , with apices enla rged in a ves icIe up to 3.0 ust:
in d iamc ter; ph ialides bottle shape d, in clus te rs of2-8 eIeme nts in the ve rt icil,
mostly 7- 9 Jtm by 2.3 -3.4 utn , abru ptly tapere d to a ve ry sho rt, narrowed
ncck ; conidia globose to subglobose, sm oo th wall ed wh en observed under th e
light microscope (fig. 92, B), a nd deIicat ely ro ughe ned whe n observed under
(h e SE M (plate 16 , top : right) , u su all y 2.3 -3. 0 Jtm by 1.8 - 2.5 Jtm ; conidial
cha ins stro ngly divergent a nd sho rt (fig . 92 , C).
Colon ies upon C YA (colour plate 92, B and b) growing restri ctedl y,
.utaining a di am et er of abou t 30 mm in 14 days at room temperature
(20- 25 C ) , consis ting of a to u gh, cIose-tex tured ba sal feIt, with m argin al and
subce ntral a reas strong ly suleate in a radial patt ern , a nd central areas
irr egul arl y folded and slightly raised in a craterifor m patt ern ; very poorl y
sporing, remaining persistently wh ite ; od our and exudate lacking ; d iffusin g
pigm en t abse nt ; colony reverse (colour plat e 92, b ) stro ng ly pulling apart th e
underl ying agar a nd deveIoping cinna mo n shades; penicilli esse n tially as
desc ribed abo ve .
Colon ies upon MEA (colour pIate 92 , C a nd c) atta in ing a d iamet er ofabo ut
25 mm in 14 d ays at room te m pe ra ture (20 - 25C), sim ila rly textu red as
abo ye , but with less radial fu rrows ; centre irregularl y fold ed in a crateriform
pattern ; very poorl y sporing, re maini ng persistently white; ex udate a nd odo ur
lacking ; diffusin g pi gm en t abse nt; colony reverse (colour plate 92, c)
deveIopi ng yello wish cinnamo n shades; penicilli essent ially as described aboye.
The spec ies description is cente red up on the typ e strain CBS 215.28 = CM I
40,59 1 = ATcc l0, 449 = NRRL 2,111 = IF07 , 724 = QM 7,566 = IJFM7054 ,
isolat ed by K . Zaleski from pine for est soil , Poland , 1927 and upon Thorrr ' s
notes rep or ted in his Monogr aph ( 1930, p . 366), sin ce th e spec ies is di fficult to
mainta in in culture and does no longer represent ade q uate ly the typ e whe n
first isolated, a t least in cult ural appearance.
' Colonies on neutral Czapek's agar characteristically depressed in the centre and
4 \J'"
eR.
vYlir il
100)Jm
Conldla globose, thlck - and smoot h w allod, yollow , 2.2tm In dlameter, separated
or formi ng short chains.
Cleistot hecia and sclerotia lack ing.'
(Translated fr om the Portuguese by the authorl .
a uain ing a d iumctcr 0 1' 20 IlIIlI in 11' days a t roo m tc m pernturc (20- 25C) ,
cons isting 01' a close-tcxtu rcd basal rei r, dcnsely vclvct y , plano, not u rrowcd,
with a few sca ttered tu fts 01' white sterile loccose hyphae in the centre; heavily
spo ri ng thro ughou t in very dark blu e- green shades (Sguy, PI. XXX, 491);
exudate and odour lacking ; amber-coloured pigment slowly diffusin g into thc
su rro u ndi ng agar; colony reverse (colour plate 93, c) in yellow-orange shades;
peni cilli esse nt ially as des cr ibed aboye, with conid iopho res arising primarily
from the subst ratum.
T he species description is centered u pon the type strain CBS 252.55 = ATce
12,068 = QM 6,946 = IJFM 7059, isolat ed as a culture contam inant in 1954 by
A . Batista and H . Maia, Institute 01' Mycology, U niversity 01' R ecife ,
Pern ambuco , Brazil . It is act ually kn own only from th e type strain .
.'
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Penicillium baradicum Baghdadi - Nov. Syst. Niz. Rast. pp. 107-108. 1968.
cast showing th rou gh in th e centre ; pcni cilli essc ntiully as dcscribcd , hut with
lon ger con id iophores and with con id ial cha ins formin g well -d efincd di ve rgen r
colum n s.
The species description is centered up on th e type strai n C BS 416 .69 = V K M
F-1,069 = C M I 140 ,336 = IJ FM 7106, isolat ed by V . C h. Baghdadi from Syri an
desert soil near Irna , in th e Damascu s region , Sy ria, M arch , 1964 . lt is known
only from th e type strain.
1~~~
L-----I
~ 100}Jm
R.
Colonies up on C A (co lour pla te 95, A and a) growing restrictedl y , attain ing
a diamet er al' 20 mm in 14 days at room te m pe ratu re (20-25C), con sisting 01'
a to ug h, close -tex tured basal felt, abru ptly rai sed , ove rlaid by a den se ne twork
01' ste rile white hyphae influen cin g greatly the colony a ppearance, lending it a
lanat e as pect; undulating m argin surro unde d by a narrow fringe 01' submerged
hyphac extc nd ing about 1 mm bcyond th c ae rial growth ; colony surf acc
irrcgula rly wrinklcd a nd rais cd; medium sp oring underncath the network 01'
rcri nl hyphae in blue-grc cn shades (Sguy, PI. XXX, 437); exudate and odour
lackin g; pal e amber-coloured pigment diffusing into the surro u nd ing agar;
colony rcv erse (colour plate 95, a) in cinnamon shadcs; pcnicilli typi cally
sim ple (appearing monoverticillate) and strongly divaricatc (fig . 95, A),
cunsisting al' verticils 01' branches and/or metulae al' unequal length, strongly
d ivcrgent , with conidiophores smooth wallcd, septate, arising primarily from
1he su bstra tu rn or from trailing hyphae, ranging from 50 to 300 Lm in lcngth
by 2. 5- 3.0 Lm in diameter, with apices (in simple penicilli) cnlarged in a
vcs icle up to 6 Lm in diameter; branche s up to 100 Lm in length by 2.0 -2.5 Lnl
in diameter; metulae, 30-60 tun by 2.0- 3.0 uu, with apices enlarged in a
vcs icle up to 6 .0 Lm in diamet er; phialides bottle shaped, tapering to a short,
narrow neck, mostly 13-15 Lm by 3. 0 - 4.0 Lm; con id ia globose to su bglobose ,
xmooth walled or nearly so wh en observed under th e light mi croscope (Iig. 95 ,
B) but appearing delicately granular when obs erved under the SE M (plate 16,
hottorn : left) , mostly 2.9-4 .0 ust: by 2.5-4 .0 Lm ; conidial cha ins forming loose
div ergent columns (fig . 95, C).
Colonies upon CYA (col our plate 95, B and b) atta ining a diamet er 01' 30
111m in 14 days a t room temperature , consisting 01' a tou gh, close-tex ture d basal
k it, appearing de eply velvety, radiatly wrinkled a nd rais ed , with undulating
margin entire; at first white, th en be coming coloure d in blue-gr een sh ades
sta rting from the centre (Sguy, PI. XXX , 437 , 438) with th e rip ening al'
conidia ; exu date and odour lacking; pale am ber -coloured pigment diffu sin g
i nto th e surrounding agar; colony revers e (colour piat e 95, b) in pale yellowor ange shades ; penicilli essentia lly as de scribed aboye, with conidiophores
a rising mo stly from trailing hyphae.
Co lon ies upon MEA (colour plat e 95, C and c) attaining a di amet er 01' 30
mm in 14 days a t room tem peratu re (20-25 C) , co nsistin g 01' a looser textured
ba sal felt, velvet y , slightly zonate , plane with a thin margin surrounded by a
fringe 01' submerged hyphae ex tend ing ab out 2 mm be yond the aerial growth ;
slightly rai sed in the centre ; heavily spor ing throughout in deep blue-green
sha des (Sguy , PI. XXX, 438) except in th e centre, which is overlaid by a thin
network 01' floccose aerial hyphae ; odour faint , indefinite ; ex uda te lacking; no
coloure d pigment diffusing into th e surrounding agar; colony reverse (colour
plat e 95, e) in oran ge shade s with a gr een cas t showing through in th e centre;
peni cilli essentially as des cribed aboye but with conidial cha ins forming divergent compact columns.
The species de scription is ce nte re d on the type strain C B S 415.69 = VKM F1075 = CMI 140 ,338 = IJFM 710 5, isolated by V .Ch . Baghdadi from Syrian
desert soil near Irna , in th e D amascu s region , Syria , March, 1964 . It is known
only from th e typ e strain.
96. Penicillium damascenum Baghdadi: lig . 96, colour pla to 96, and SEM
plate 16 , (bott om: rig ht).
w.w.w..u
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C\
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100 urn
Penicil!ium damascenum Baghdadi - Nov . Syst. Niz. Rast. pp . 101 -102, 1968.
margin ; pen icilli are e itber str ict ly sim ple (appeurin g m un ov ert icillat e) a m i
01' 2 - 5 m etulac 01' cq u al or un equal
Icn gth, typically di vergent (fig . 96 , A) ; m on overticillate penicilli are usu ally
p rcd ominant in th e margin; co n id iopho re s a r ising eithe r from th e su bs tratu m
a nd frorn trailing hyphae, smooth wall ed in our obse rvat ion (rough walled in
Baghdadi's original d iagnos is) , with a pice s e nla rge d in sim ple struc tures up to
:1. 5 /lm in di amet er, m ostl y 200 -250 /lm b y 1.8- 2.5 /lm when borne fro rn th e
su bstratu m , and shorter, up to 50 /lm in length when borne from aerial
hyphae ; m etulae usu all y 10 -20 /lm by 2.0 -2 .5 tuu , with a pices e nla rged in a
vesicle up to 3. 0 /lm in diamet er; phialides b ottle shape d, a b r up tly tapered to a
sho rt n eck , co m m only 7-10 /lm b y 2. 5 -3 .0 usu , in compact whorls 01' 2 -8
c lerne n ts in th e ve rtici l; coni d ia glob ose , cons p icuous ly sp inu lose (fig . 96, B,
a nd SEM plate 16, bottom: right), 2. 8 -3.5 /lm in diamet er; co n id ia l chains
lorming loo se di vergent columns (fig . 96 , C ) .
Colon ies upon CYA (colou r pl a te 96, B a n d b ) a tt a in ing a di amet er 01' 40
mm in 14 days a t ro om temperature , (20- 25C) , co ns isting 01' a close-textured
basal felt , velve ty in a p pearance, d eeply radiately furro wed a nd rais ed in th e
ce n tral a rea, with a wh ite undulating m argin surrou nde d by a frin ge 01'
sub merged h yphae ex te n d ing a bou t 1 mm beyond th e ae r ia l growth; heav ily
spor ing through out , a t fir st in brownish green colours (Sguy, PI. XLVII ,
702 ), turning to blue- gr een shade s (Sgu y, PI. XXX , 443) with th e r ipen in g 01'
co n id ia; odou r faint , ' mou ldy'; exuda te a b u n dan tly produ ced , as ye lloworan ge dro ps; a m be r pigment di ffu sing in to th e su r ro u n d ing agar; co lony
re ve rse (colou r plate 96 , b) in cin namo n to oran ge sh ades; penicilli essen tially
as de scribed a boye .
Colonies upon M EA (colour plate 96 , C a nd c) a tta in ing a di amet er 01' 25
m m in 14 days a t ro om temperature (20 - 25 C), co ns isti ng 01' a looser textured
felt , velve ty in a ppearance, not furrowed , plane , w ith even , thin m argin su rro u nded by a wide frin ge 01' submerged h yphae extending abo u t 2 mm beyond
th e ae ria l gr owth ; hea vily spo r in g throughout in blue-gray sha des (Sguy, PI.
X X X I, 463 , 464) ; ex u date a nd od our lacking ; amber-coloured pi gment di ffus ing int o the su r rou nd in g a gar; colony reve rse (co lou r plat e 96 , c) ye llow ish
with a green ish cast sho wing th ro ugh; penicilli essen tia lly as described a boye
but with con idiop hores mostly borne primarily from the su bs tra te, a nd sim ple
p enicilli predominantly encou n te re d over th e divaricat e ; di varicat e peni cilli
usu all y a r ising fro m trailing h yphae .
The species de scr ip tion is ce n te re d upon th e type strain C BS 411 .69 = V K M
F-1070 = C M I 140,337 = IJ F M 7104 , isola te d b y V. C h . Ba ghdadi fro m Sy r ia n
d esert soil n ear Irna , in the D amascu s regi on , Syria , March , 1964. It is kn own
on ly from th e type strain .
t ypically diva ricatc , co ns isting uf verticils
08
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Penicillium gorlenkoanum Baghdadi - Nov. Syst . Niz. Rast. pp. 97-98. 1968.
Colon ies upon CA (colour pla te 97, A and a) growing rather restrictedly ,
attaining a diameter of 25 mm in 14 days at roo m temperature (20 -25 C),
co nsisting of a tough , close -text ured basal felt , ty pically velve ty , radiately
sulcated by shallow furrows and raised towards the centre whi ch is umbonate ;
thin, u ndula tin g margin surrounded by a narrow fringe of submerged hyphae
extending less than 1 mm beyon d the aerial growth; heavily sporing
througho u t in blue-green shades (Sguy, PI. XXXIII , 483 , 484) , especially in
the margin: exudate abundantIy produced as large orange-coloured drops,
obs curing in central and subcentral area s th e con idial structures; odo ur
lacking ; ve ry pale a mber-coloured pigm ent diffu sing slowly into the surround ing
agar; colony reve rse (colour pIate 97, a) strong ly pulling a nd cracking the agar
away, in pale burnt sienna brow n shades (Sguy , PI. X LVII , 694, 695);
pen icilli ty pically diva ricate (fig. 97, A) , consisting of ve rti cils of 2- 3 metula e
of uneq ual len gth fairly divergen t ; coni dio phore s smooth wa lled in our
observation (in Baghdadi' s orig inal descrip tion rou gh wa lled conidiopho res
were also enco untered), mos tIy 150-1 000 .tm in len gth by 2 .5 -3 .5 tut: in
98 . P enicillium harmonense Ba ghd adi : fig. 98, colour plate 98, and SEM
u.w..U.w.J
10 jirn
O O
00
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0O0
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L.--J
100 prn
mooth wall ed or nearly so wh en obs crv ed undcr th e light mi croscope (Iig. 98,
B) but delicatc1y roughcncd wh en obs ervcd undcr th c SEM (platc 17, top:
1'Khl), ranging from 3.0 to 4 .5 p.m by 2.3 -3.5 p.m, in diameter; conidial chains
Iunnin g Iairly well-defined divergcnt colum ns (fig. 98, C) .
C olonics u pon CYA (colour plate 98, B and b) attaining a diameter 01' about
"o mm in 14 days at room temperature (20-25 C), consisting 01' a closei cxturcd basal felt, de epIy velvety to lanate , sulcated by numerous radial
lu rrows, gradually raised towards the centre; white, wide undulating margin
surrounded by a fringe ofsubmerged hyphae extending about 2-3 mm beyond
1he acrial growth; heavily sporing throughout in deep blue-green shades
(S(~ g u y , PI. XXX, 447); exudate abundantly produced, collecting as largc,
bri ght yellow-coloured drops ; odour strong, 'mouldy'; bright yellow-colourcd
pigm cnt diffusing into the surrounding agar; colony reverse (colour plate 98,
1 in bright orange shades; penicilli essentially as described aboye.
C olonies upon MEA (colour plate 98, C and c) spreading broadly, attaining
I diameter of 55 mm in 14 days at room temperature (20 -25C), consisting of
a looser textured basal felt overlaid by a loosely woven network of floccose
ic rial hyphae , lending a lanate appearance to the colony surface; wide , thin,
whit e margin surrounded by a fringe of submerged hyphae extending up to 5
111111 01' more beyond the aerial growth; colony surface plane , not furrowed or
very slightly radiately wrinkled; heavily sporing throughout in blue-green
shades (Sguy, PI. XXX, 443 , 444); conidial areas partially obscured by deep
scc iors and tufts of floccose aerial hyphae; exuda te and odour lacking; no
coloured pigment diffusing into th e surrounding agar; colony reverse (colour
plate 98, e) developing red shades; penicilli essentially as described aboye,
commonly arising from conidiophores usually borne upon aerial hyphae.
The species description is centered upon the type strain CBS 412 .69 = VKM
10'-1,078 = CMI 140,340 = IJFM 7103 , isolated from Syrian desert soil near
lrna , in the Damascus region, Syria, by V .Ch. Baghdadi, March, 1964. It is
known only from the type strain .
99. Penicillium sizovae Baghdadi: fig. 99, colour plate 99, and SEM
plate 17, centre: left).
Penicillium sizovae Baghdadi - Nov. Syst. Niz. Rast. pp. 103-104. 1968.
00
8 00
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00
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fR .
throughout in blue-gray shades (Sgu y , PI. XXXIII , 493) with conid ial areas
partiall y ob scured by th e m ycelial tloccose overgro wth; colony surface
rad iately fu rro wed a nd slightly raised tow ards th e centre ; ex uda te a bundantly
produced, as crys ta l clear, uncoloured drops ; odour lacking ; no coloure d
pi gm ent diffu sin g into th e surrounding a ga r ; colony reverse (colour plat e 99, a)
in light cin na mon shades; penicilli cha racte ristically divaricat e (fig. 99 , A) ,
co ns isting of verticils of fairly equal metulae , conspicuo us ly divergent , but
suggesting th e Biverti cillata- symmetrica in pa ttern, owing to th e regular
a rrange ment of metul a e at th e tips of th e cond iophores; sim ple penicilli borne
on lat er al branches from conidiophor es are not in freguently enco u ntere d;
co nid iophores smoo th walled , mostly arising fro m the ba sal felt , com mo nly
ra n ging from 50 to 200 IJ-m by 1.5- 2.8 IJ-m with a pices enlarge d in a ves icle up
to 5.0 IJ-m in di ameter ; m etulae in whorls of 2- 4 eleme nts in th e ver ticil ,
rangin g from 11 to 25 IJ-m by 1.5- 3.5 IJ-m , some times of eq ual len gth but
u suall y of unequal size; phialides bottle shape d, abru ptly tapering to a sho rt,
narrowed n eck , commonly 9- 14 IJ-m by 3 .0 -4 .0 IJ-m , in crow de d clus ters of
4-12 elements in the ve rticil; conid ia globose, com pa ratively sm all , sm ooth
walled whe n obse rve d under th e ligh t mi croscop e (fig. 99, B) but delicately
" .5-:LO tlTl in di arn eter ; con id ia l cha ins formin g lon g, well-defin ed divergcnt
1'0 111 IJIns ,
100 . Penicillium nooae-caledoniae Sm ith : fig. 100, colour plate 100, and
SEM plate 17, (centre right ).
Penlcillium novee-celedoniee Smith - Trans. Brit. Mycol. Soc o48: 273. 1965. See
also M. M . Kulik, 'A Compilation of Descriptions of New Penicillium Species'.
Agric . Handbook No. 351. Agric. Res. Ser. U.S. Dep. Agric. p. 58-59. 1968.
.".
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fR. .
!,-tl t
100jJm
Smith's original diagnosis follows, since this adequately presented the species in
question when first isolated:
'Colonies on Czapek's agar growing rapidly, bluish gray-green at first, becoming
grayer and duller, with narrow white margin during the growing period, thick
velvety to matted floccose, with reverse at first buff to dull pink, becoming pinkish
brown; penicilli usually with terminal verticil of metulae, these being commonly of
different lengths in the same verticil, sometimes with a secondary branch from the
next lower node of the conidiophore, typically divaricate; conidiophores rough,
mostly 3 tm in diameter; metulae 10 to 15 tm by 3 tm, often widening somewhat
towards the summit; phialides short-pointed, 8 to 10 tm by 2.2 to 2.5 tm; conidia
slightly rough, subglobose, 2,5 to 3.0 tm in diameter; conidial chains at first more or
less parallel, soon becoming tangled.
Isolated from soil, New Caledonia, by B. Huguenin, 1964.'
O ur n ot es follow :
Colon ies upon C A (co lour pla te 100 , A a nd a) a ttain ing a di amet er o f 25 - 30
mm in 14 da ys a t room te mperatu re (20 - 25 C), cons ist ing of tou gh , close-
tcxtu rcd basal Ielt., vc1vcty to sublan at c in a ppcara ncc , with whitc dcnticulatc
m urgin , surrounded by a frin gc 01' su bme rgcd hyphac cxtcncl ing ab out 2 mm
bcyond thc acrial growth ; colony su rface slightly su lca ted by shallow radial
Iurrows; hcavily sporing throughout in blu e-gray sha de s (Sg uy, PI. XXI ,
~1 ()1I ) ; cxud a te and odour lacking ; pal e vinace ous coloured pigm ent diffusing
Il lo the surrou nd ing ag ar; colony reverse (colour plat e 100, a) in purple-brown
xhudcs ; penicilli confor m with Smith's origin al diagnosis (fig . 100 , A) ,
CllIlsisting 01' con idiophores co nspicuously wa rty, ranging from 50 to 300 .tm in
lcn gth by 2.8-3 .5 .tm in diamet er, septa te, with terminal verticils ofmetulae 01'
u ncqual len gth , com mo nly 18- 25 iut: by 2. 5- 3. 5 uru , with a pices enla rge d in
1 vcsicle up to 4.0 tui: in diamet er; phialides bottle shaped, tap ering abruptly
lo a short narrowed neck , 10-14 .tm by 3 .0 - 4 .0 tui ; con id ia globose to
Il lll>globose, appe a ring smooth 0 1' nearIy so when observed under th e light
iu ic roscope (fig . 100, B) but delicat ely rou ghen ed when obse rved under the
SJo: M (pl at e 17, cen tre: right); co n id ial chains at first loosely divergent , soon
hccorning tangled (fig . 100, C ) .
Co lon ies up on CYA (colour plat e 100, B and b) spreading broadl y , att a in ing
I d iameter 01' 50 mm in 14 days a t room temperature (20 -2 5 C ), co ns isting 01'
a close-textured basal felt, deeply velvet y , ove rIa id by a th in m ycelial
uvcrgrowth cons isting 01' a closely woven network 01' fin e aerial hyphae
iu fl uenc ing gre a tly the colony a ppearance , lending to it a subla na te aspec t;
c: olony surface radiat ely furrowed , with white margin surrounded by a narrow
Iringe 01' submerge d hyphae ex tend ing abo ut 1 mm bey ond th e aerial grow th;
iu cdium sporing in pale brownish gree n shade s (Sguy , PI. XLVII , 693);
exudare and odour lacking ; reddish brown- coloured pigm ent diffu sin g into th e
xurrou nding agar; colony revers e in sienna reddish brown colou rs (co lour plat e
100 , b); penicilli esse ntially as described above .
C olonies upon MEA (co lour plate 100 , C and c) attaining a diamet er 01' 35
111111 in 14 day s at room temperature (20-25 C), cons isting 01' a close-t extured
basal felt , typi cally velvety to su bla na te , owin g to a loose ove rgrowth 01' floccose ae rial hyphae overIaying the colony surface; margin entire ; heavily
sporing throughout in mou se gray shades (Sgu y , PI. XXXV , 523); exu da te
nnd od our lacking; colony reverse (colour plat e 100 , c) developing typi cal
stra wbe rry red sha de s; sim ila rIy coloured pigm ent slowly diffu sin g into th e
sur rounding aga r ; penicilli esse ntia lly as described aboye, with con id iophores
a rising as short bran ch es fro m th e netw ork 01' tr ailing hyphae.
The spec ies description is centered upon the stra in IMI 140 ,441 = IJFM 7196,
rcceived in 1979 from th e Com m onwealth M ycological Institute, K ew , Surrey ,
England , and isolat ed by MI' Ian H . Pallet from de cayin g Lycopersicon sculentum
roots in soil near Betchw orth, Su rrey , U.K . The typ e strain IMI 104 ,1 56 ,
isolated from N ew Caled oni an soil by B. Hugu enin in 1964 , unfortunately lost
Irorn th e CMI cu ltu re collection, could not be exam ined by the au thor 01' th e
,
"
"
,e
Ll..W..W.J.JJ
10jJm
"
"
'.
000
00 0 B
O
0 00 0
O O 0 0
00
tt
100jJm
album
Ramrez et
Martnez
IrI101lnt, as min u te d roplct s c n mcs hc d in the m ycc lia l 1'1.:11 ; no co lou rcd pigrncnt dil'l'lIs in g into th e su rr ou nd ing agu r : colo ny re ve rso (co lou r plate 101, a)
11 palo cin namo n sha de s with rna rgin yellow ; pcni cilli typically divaricate (li g .
101 , A) com monly cons isting 01' verticils 01' 2 -:~ me tu lae of unequal len gth ,
umc timcs with a seco ndary branch , ran gin g fr orn 16 to 20 /lm by 2.8 - 3 .5 uiu ;
cu uid iop horcs with wall s co ns p icuo us ly warty, a risin g frorn th e b a sal felt,
n lllslly 50 - 150 /lm by 3. 0 - 4 .0 urtv; phialides bottle sh ap ed , tapering to a sho rtpui utcd ncck , in clus te rs 01' 3 -9 eleme nts in the ve rt icil, co m mo nly 10- 12 /lm
liy :1.0- 4.0 psi ; co n id ia globose to sub globose , mostly 2. 5 - 3. 5 tut , smo oth
wullcd 0 1' nearly so wh en observe d under th e light mi croscope (fig . 101 , B) bu t
k-lica tely rough ened whe n obse rve d under the SEM (pla te 17, bottom : left);
ru uid ial chains at fir st more 01' less div ergent , becoming tangled in age (fig.
lo 1, C) .
Colon ies upon C YA (co lou r plate 101 , B a nd b ) sp read ing broadly , attaining
I d ia rn et er of 55 mm in 14 d a ys a t room temperature (2 0 -2 5C), co ns ist ing 01'
I rlosc -textu re d ba sal felt, ove rlaid by a dense m ycelial network 01' ae r ia l
hyphac, with shallow rad ial fu r rows n ear th e m argin ; gro w in g m argin
MlII'l'Ou nd ed b y a fr inge 01' sub merged hyphae , exte ndi ng a bo ut 5 mm beyond
111(' ac ria! gro w th; ve ry poorly sp oring, rema ining persistently white ; odo ur
,ll'O ng , suggesti ng mushrooms; ex u date lacking; pale a m be r-colou re d p igment
d illu sin g int o th e su rro u n d ing agar; colony reverse in reddish brown shades
(I'lllou r pla te 101 , b); penicil!i esse n tially as described a bo ve .
Colon ies upon MEA (co lou r plat e ,101 C a nd e) gro win g rather restrict edly,
II tta ining a di amet er 01' 35 mm in 14 da ys a t room temperatu re (20 -25C),
l'lIlIsist ing 01' a close- tex tu re d b asal felt , de eply lanat e , with ce n tre rai sed , w ith
c vc n margin su r ro u nde d by a frin ge 01' sub mer ged hyphae exte nd ing a bo u t
1-4 m m beyond th e aeri al growth; colony surface not furrowed , rem aining
pc rsistently white ; sparsely sporing , with con id ial structures a r ising as shor t
h run ches from ae r ial h yphae ; ex u da te and odou r lack in g ; colony reverse
('olour plat e 101 , c) at fir st in cin na mon sh ades, slowly developing the typi cal
sI ra wbe rry red co lou r characte r istic 01' th e spec ies.
T he va r iety duplicat es the typ e spec ies in colony texture , rat e 01' growth a nd
in the det ails 01' m orphology , but differs from it in th e persistently white obve rse
lll' (he colon ies upon al! subs tra ta tested .
The description is ce n tere d upon strain IJ FM 7184, isolat ed by th e a u tho r 01'
the curre n t stu dy in 1979 fro m the a ir 01' M adrid , Spain . It is at present kn own
nnly from th e type strain .
102. Penicillium citreo-otrens Abe ex R amrez: fig. 102, colour plate 102,
and SEM plat e 17, (b ottom : right).
Penicil/ium citreo-virens Abe - J. Gen. Appl. Microbiol. Tokyo. 2: 87-88. 1956. See
o
-
00
O
000
-:.n 8 0
OUO O
O
10jJm
y~
also G. Smith, Trans. Brit. Mycol. Soco 46: 332. 1963; also M .M. Kulik, 'A
Compilation of Descriptions of New Penici/lium species'. Agric. Handbook No.
351. Agric. Res. Ser. U.S. Dep. Agric. pp. 28-29. 1968.
cream shades; becoming typically green-ollve In the central ares and sepia in the
marginal areas; surrounding agar usually colourless; conidiophores arising mostly
from the substratum or from the basal felt, mostly 60-250 p.m in length by 2.5-2.7
p'm in diameter, with apices 2.8-4.7 p'm in diameter and with walls smooth or nearly
so, usually unbranched; penicilli typically biverticillate and asymmetrical, consisting
of a terminal group of 3-5 compact or slightly divergent metulae that measure
about 12.5-25.0 p'm by 2.5-3.7 p'm (apices commonly enlarged to 2.6-4.7 p.m),
each supporting a cluster of 3-7 compact or parallel sterigmata (phialides)
measuring about 7.5-11.9 p'm by 1.7-2.5 p'm, and bearing conidia in columns or
loosely tangled chains up to 60-120 p.m in length; conidia globose to subglobose,
mostly 2.3-3.3 p'm, smooth walled or nearly so; and conidia walls delicately
roughened (spines less than 0.1 p.m) as shown in electron microscopy.'
"
pluu c surrou nded by a fringe 01' submcrgcd hyph ae extcn d ing abo u t 1 mm
licyond thc acria l growt h; su bmarg ina l a nd ce n tra l a rcas dccply radi atcly
1'1I1'I'Owcd and de p res scd in th c ce n tre , co lon y su rfacc co ns pic uo usly zanate ;
hunvi ly spo rin g throughout in d ccp blu e-grecn sha des (Sguy, PI. XXXI,
;1(11); cxud a te abundantly produccd , cs pec ially in th e ce n tre as large, cr yst al
C1l:11 l', colou rless drops; odou r la ckin g 0 1' indefinitc ; nu colou re d pigment
d lllu sing into the surrounding ag ar ; colony revcrse (col our plate 102 , b)
d evc lop in g ora n ge sh ades ; penicilli essen tia lly as described .
:o\on ies upon MEA (col our plate 102 , C and c) sp reading broadly ,
lll /lilling a di ameter 01' 55 mm in 14 days a t room temperature (20- 25C),
c't1l1 sis ting of a loo ser textured basal felt, slightly zona te, typically velvety , with
1II Ill'g in thin , su r ro u nde d by a wide frin ge 01' su b merg ed hyphae ex te nd ing
rho u t 5 mm beyond th e aerial grow th ; colony su rface not furrowed , plane w ith
(' (' 111 re sligh tly umbon ate; hea vily spo r ing thro ugho u t in very dark blue- green
hades (Sguy , PI. XXX , 446) ; ex udate and odo u r lacking; coloured pi gment
d iflus ing into the su r ro u ndi ng aga r is not observed; colony rev erse in crea m
Mhades with a d a rk green cast sho wi ng through in the cen tral area ; penicilli
,'sselltially as described abo ye.
T he species description is ce nte re d upon th e type strain IFO 6 ,0 30 = ATCC
1ll , ~nt = CBS 320.59 = CMI68 ,2 30 = IJFM 7069 , isolated fromJapanese soil
hy S . Ab e in 1952 , a nd many othe r strains isolat ed b y th e a u tho r fro m Sp anish
stli1.
Penicillium citreo-uirens is in clu de d in th e Asymmetrica-divaricata sub-sec tion
s iucc the m aj ority 01' th e penicilli sho w this b asic pattern. M any struc tu res,
huwcver , ap pear clearly monoverticillate w ith individual penicilli borne singly
1111 sho rt branches. While we rega rd th e present placement as co r rec t, th e
p robabl e relationship 01' th e species to th e M onoverticillat a , possibl y as a
t rn nsi tio nal for m, sho u ld not be di sregarded .
Peni cilli cha rac teristically div aricatc , wilh eo nid ial chains typically ad herent
in loase di vergcn t columns .
Con id ia globose to subglobose, with wa lls rang ing from smooth or nearly s
to conspicuo usly roughe ne d, de pe nd ing upon the spe cies.
K ey to species
Paga
1. Penicilli with the divaricate character well marked; growing rather
restrictedly, at least upon some substrata; with metulae scattered on the
conidiophore, or usually aggregated into true verticils. Colonies
develop ing typically pale blue-green or gray-green shades with the
ripening of conidia ; colony reverse often brightly coloured. Colonidial
chains tending to produce compact columns , at least in young cultures;
conidia globose to subglobose.
P. canescens series ... . 283
A. Colony reverse developing deep red or brown shades; penicilli
strongly divaricate , not tending towards ramigenous.
1. Colonies up to 0.5-1 .0 mm deep, with surface loase, more or less
lanate; conidial areas in dull blue-green shades; conidia about
2.0-2.5 Jlm in diameter; colony reverse orange, becoming brown in
age.
P. canescens Sopp .. . . 285
2. Colonies deeply lanate, up to 2.0-3.0 mm deep; conidial areas in
brighter greenish glaucus shades; conidia about 3.2-3.6 Jlm in diameter; colon y reverse in deep red shades near maroon.
P. nalgiovense Laxa .. .. 287
3. Colonies appearing typically velvety.
a. Conidiophores
smooth
walled;
conid ia
conspicuously
roughened.
P. turolense Martnez et Ramrez . . . . 293
b. Conidiophores delicately roughened; con idia strongly echinulate .
P. murcianum Ramrez et Martnez . ... 291
B. Colon y reverse orange , in dull peach shades or colourless, not
developing dark colou rs; penicilli often appearing somewhat
ramigenous.
P. jensenii Zaleski . .. . 289
Ifllgiovtmse Lax a , and P. [ensenii Zaleski, AlI 01' thcm tcnd to produ ce conicl ial
chuins in loose colu mns, all produce lon g conidiophores, often with I'a irly wellurgunized terminal peni cilli , a nd all prod uce globoso to subglobo se conid ia .
'I' wo add itional species have been inclu dcd hcre in the cur rent stud y, namcly
l'enicillium turolense M art nez et Ram rez , an d P. murcianum R arnfrez el
M ur tncz .
10:1. Penicillium canescens Sopp : fig. 103, colour plate 103, and SE M
plate 18, (top : left ).
..:.~:
.:.
:::;
(f~
(1:&
~
~g;)
iJ.~r) (~ (~
i::::.' ~
;".:
A~~1~
1~
;'.:
--:-
e"R,
Penicillium canescens Sopp - Monographie, pp. 181 -182. 1912. See also Ch.
Thom, 'The Penicillia', pp. 347-348.1930; also Raper and Thorn , 'A Manual of the
Penicillia', pp. 316-319. 1949.
~1Il>
with surlacc more or less lana te consisting 01' a loose -tcxturcd network 01'
interwoven vcgetative hyphae arising from a close-tcxtured, compact basal felt ,
lending a deeply floccose appearance to the colon y surfacc; thin margin 2-1mm wide, surrounded by a fringe 01' submerged hyphae extending about 2 -3
mm beyond the aerial growth; colony surface convex; fairly abundantly
sporing throughout in pale gray shades (Sguy, Pi. XVI, 235); exudate
limited, pale amber, as minute droplets enmeshed in the mycelial felt; odour
lacking or indefinite; no coloured pigment diffusing into the surrounding agar;
colony reverse developing yellow shades (colour plate 103, a); penicilli variable
in sizc (fig. 103, A), borne either upon conidiophores arising directly from th e
substratum and varying in length up to 500 ,tm by 2.5-3 .5 ,tm in diameter, or
upon short branches from aerial hyphae, with walls ranging from
conspicuously warty to delicately roughened or nearly smooth , consisting 01'
variously arranged divergent structures, forming verticils 01' 2- 3 metulae
usually 10-25 ,tm by 2 .5-4.0 tui, bearing clusters 01' phialides 01' 4-12
elements in the verticil, mostly 6-10 ,tm by 2.5-3 .5 tut, bottle shaped,
terminating in a tapered, short narrowed neck; conidial chains tending to
adhere into loose columns at least in young cultures, but becoming more or less
divergent in age (fig. 103, C); conidia at first ovate to subglobose becoming
globose at maturity, with walls conspicuously roughened when observed under
the light microscope (fig. 103, B) and showing a definitely warty surface when
observed under the SEM (plate 18, top: left), usually 2.5-3.0 ,tm in diameter.
Colonies upon CYA (colour plate 103, B and b) spreading, attaining a
diameter 01' 50-60 mm in 14 days at room temperature (20-25C), consisting
01' a deeply velvety to lanate felt, deeply radiately and irregularly furrowed,
with concentric ridges, with centre depressed in a crateriform pattern; with
white even margin 5 mm wide surrounded by a narrow fringe 01' submerged
hyphae extending about 1 mm beyond the aerial growth ; abundantly sporing
throughout in brownish gray shades (Sguy, Pi. XXIX, 428); exudate limited,
as small droplets enmeshed in the mycelial felt; odour faint, 'mouldy'; reddish
brown pigment diffusing into the surrounding agar; colony reverse (colour
plate 103, b) developing deep brown, almost black shades ; penicilli essentially
as described aboye .
Colonies upon MEA (colour plate 103, C and c) growing restrictedly,
attaining a diameter 01' 25 mm in 14 days at room temperature (20-25C),
consisting 01' a loose-textured basal felt, lanate, somewhat funiculose, about 1
mm deep, not furrowed, with margin irregularly dissected in a denticulate
pattern surrounded by a narrow fringe 01' submerged hyphae extending about 1
mm beyond the aerial growth; heavily sporing throughout in mouse gray
shades (Sguy, Pi. XXXV, 523); exudate and odour lacking; reddish browncoloured pigment diffusing into the surrounding agar; colony reverse (colour
plate 103 , c) developing dark reddish brown colours; penicilli essentially as
describc d a bo ye but sho w iug a tc ndency lo p rod uce mo re co m puc t colu mna 01'
cunid iu.
'I'hc spccies d escr ip tio n is ce n tc rc d upo n strni n CIIS :~ 00 .4 8 - CM128 ,260 10,41 9 = NRRL9 10 = Q M 7550 .. I./F M :~ 866 , isola ted b y Miss E . Dale in
1';Ilf.{l an d , fro m soil, in 1912, cited b y T hom in 1930 a nd by Raper a nd T hom
i 11 t 949 as representing th e spcc ics.
l'enicillium canescens appears to be som ewha t tr ansitio nal betw een P.
j anthinellum a nd P. nigricans. It gro ws more res trictedly , at least up on M EA
I ha n the spe cies 01' the for rner seri es ; it prod uces deep brown rather tha n
pu rpl e-red colours in colony rev erse , suggestive 01' P. nigricans; a nd conid ia are
globose and rou gh as in the la tter series .
A'J'l: G
104 . Penicillium nalgiovense Lax a: fig. 104, colour plate 104, and SE M
.:'
o O
00
BO O O
O 00
00
O 00
i.w.I..WW
10J.lm
0 0 0
fR.,
() Cj OO O
0 0
-r:
I..-----.--J
100J.lm
Penicilli um na/giovense Laxa - Z. Bakt. Parasitenk . Inf. Hyg. 11 Abt. 86: 160-165.
1932.See also Raper and Thorn, 'A Manual of the Penicillia', pp. 319-322. 1949.
Colonies upon CA (colour plate 104, A a nd a) growing restrict cdly , a tta in ing 11
diameter of 30 mm in 14 days a t room ternpcraturc (20 - 25 C), co ns isting al' 11
fairl y closely woven network 01' vegetative hyphae lending a de eply lanat e
appearance to the colony 2- 3 mm deep, slightly radiately furrowcd, somewhat
zonate , at first white but gradually developing pale yellow- gree n shadcs wi th
the ripening of conidia; thin , eve n margin surrounded by a fringc 01'
submerged hyphae extending about 1-2 mm beyond th e a erial growth;
sparsely sporing over most of th e colony su rface , becoming yellow-green
(Sguy, PI. XXIII, 335) in fruiting areas and usually remaining white to ro sy
white in areas of purely vege ta tive growth ; exu da te abundantly produced as
large golden ye llow drops; od our la cking; yellow-coloured pigment diffusing
into th e surrounding a ga r ; co lon y rev erse in ye llow-ora n ge colou rs (colour
plate 104, a) ; penicilli strongly divaricate (fig. 104 , A) , variable in pattern ,
consisting o f occ asion al branch es, and metulae varying gr eatly in arrangc ments and in size; con id ial structures arising commonly a s short branches frorn
aerial hyphae , less frequently from th e substratum ; con id iophores ranging
frorn very short to up to 500 p.m in length by 2.8-3.0 p.m in diameter, with
walls gen era lly delicately roughened , more or less septate ; metulae mostly in
small clusters of 2- 3 elements in the verticil , usually 7-1 3 p.m by 2.8- 3.5 p.mj
phialides com p a ra tively small , bottle sh a ped , terminating abruptly, without
consp icuou s n eck , mo stly 6- 8 p.m by 3. 0 - 4 .0 tut , borne in clusters ol' 2-6
elements in th e ve rticil; conidia elliptica l, subglobose or globose, appearing
smooth walled when ob served under th e light mi croscope (fig. 104 , B) but ve ry
finely roughened when obs erved under th e SEM (plate 18, top: right), mostly
3. 5- 4. 5 p.m by 3. 0 - 3.5 tut: in diameter, sorne occasionally larger, up to 6.0 p.m
in long axis ; con id ial chains short, tending to adhere into narrow colu m ns (fig .
104 , C) .
Colonies upon CYA (colour plate 104 , B and b ) attaining a di ameter of 40
mm in 14 days at room temperature (20-25 C) , similar in texture to those
grown upon CA, but d eep er in the ce n tre and su lca te d by numerous radial
furrow s near th e margin, whi ch is su rro u n ded by a very narrow fringe of
su bmerged hyphae not exceed in g 1 mm bey ond th e aer ial growth; sporing very
poorly with colony surface remaining persistently white or turning to yellowish
tints; exud a te as very small drops em bedded in the mycelial felt, colou rle ss;
odo u r la cking; yellowish colou re d pigment diffusing into the su r rou nd in g agar;
colony reverse (colour plate 104, b) in yellow-ora n ge shades; penicilli
ess entially as described aboye .
Colonies upon MEA (colour plate 104 , C and c) growing very restrictedl y ,
co m pa ratively thin , attaining a diamet er of 20 mm in 14 days at room
temperature (20-25 C), consisting of a close-textured basal felt , deeply
vel vety to lanate , with fin ely denticulate m argin largely submerged exte nd in g
about 2 mm be yond the aerial growth, not furrowed ; colony surface remaining
persistently white in arca s 01' purcly vegetat ivo gro wth, sha ding to pale yellowgreen shad es in areas abundantly sporing, particularly in the colony centre
(S guy , PI. XXVI , 380); exudate and odo u r lacking; no coloured pigment
diffusing into th e surrounding agar; colony reverse in yellow-ora nge shades;
penicilli essentially as described ab oye but with conid ial chains lon ger a nd
columns somewha t tangled .
The spec ies description is centere d upon the typ e strain CBS 352.48 = C M I
39, 804 = ATCC 10,472 = NRRL 911 = IFa 8,112 = QM 7,600 = IJFM 7055 ,
isolated by O . Laxa from ElIischauer chee se (01' the C amembert type), in
Nalzovy, Southern Bohemia , in 1932.
105. Penicillium jensenii ZaIeski: fig. 105, colou r pIat e 105, and SEM
pIate 18, (centre : Ieft).
{~~ :~ ,:':i~
{ :;\
;fr'f r~ <' .,
L--...J
100 pm
Penicillium jensenii Zaleski - Bull. Acad . Polonaise Sci. Math . Nat . Sr. B, pp.
494-495. 1927. See also Ch. Thom, 'The Penicillia', pp . 346-347.1930; also Raper
and Thom, 'A Manual of the Penicillia', pp . 322-323. 1949.
Synonym
Penicillium chrzsszczii Zaleski -- Bull. Acad. Polonaise Sci. Math. Nat. Sr. B, pp.
464-466. 1927.
Colonies upon CA (colour plate 105, A and a) growing restrictedly , attaining a
diameter of25-30 mm in 14 days at room temperature (20-25 C), consisting
of close-textured basal felt about 500 ,tm deep , strongly folded and radiately
wrinkled, with marginal area abruptly raised and centre d epressed, with the
subcentral underlying area com m only pulling away the agar from the culture
dish and cra cking it , with colony surface appearing loos e , finely granular,
almost lanate, more or less zonate, with abruptly raised margin 1-2 mm wide;
light to medium sporing in pale yellow-green shades (Sguy , PI. XXV , 365) in
fruiting areas and u sually remaining white in areas of purely vegetative
growth; exudate abundantly produced, particularly in the subcentral and
cen tr al depression as deep orange, crystal clear, large drops; odour lacking;
orange-coloured pigment diffusing into th e surrounding agar ; colony reverse
(colour pi ate 105 , a) developing deep orange shades; penicilli typically
divaricate (fig. 105, A), often appearing ramigenous, in larger structures
commonly con sistin g ofwell-defined terminal clusters of2, 3, or more metulae ;
con id iopho res variable , ranging from smooth walled to con sp icu ou sly warty
with warts sparsely arranged on th e conidiophore wall , arising from the
substratum up to 500 ,tm or more in length by 2 .5- 3 .0 ust: in diameter , or
borne as lateral branches on trailing hyphae , u sually shorter, not ex ceeding
100 ,tm in length, with apices enlarged in a vesicle up to 5 .5 tui: in diameter;
metulae va ria b le, usually 10-18 ,tm by 2.0-2.5 tui, with a p ices infl ated in a
vesicle up to 4 .5 ,tm in diameter , always smooth walled ; phialides bottle
shaped, usually in clu sters of 3-10 elements in the verticil, mostly 8 -9 ,tm by
2.5-3 .0 tun , conspicuously tapering to a short narrowed neck; conidia globose
to subglobose , 2.0-2 .8 tut: in diameter, with walls conspicuously but delicately
roughened (fig. 105 , B, and SEM plate 18 , cen tre : left) ; conidial ch a in s
tending to adhere in well-defined loose, divergent columns (fig. 105, C) .
Colonies upon CYA (colour plate 105 , B and b) , spreading broadly,
attaining a diameter of 45 mm, consisting of a close-textured basal felt, deeply
velvety in appearance, with the surface strongly and irregularly wrinkled and
folded in a more or less radial pattern, with the central area folded in a
crateriform pattern; undulating margin surrounded by a fringe of submerged
hyphae extending less than 1 mm beyond the aerial growth; heavily sporing
throughout in blue-green shades (Sguy, PI. XXX, 442) ; exud a te and odour
lacking; amber-coloured pigment diffusing into the surrounding agar; colony
reverse (colour plate 105, b) in reddish orange to cinnamon shades; penicilli
essen tia lly as described aboye but more abundantly produced .
Colonies upon MEA (colour plate 105, C and c) growing very restrictedly,
~Ul
altaining a diameter 01' 20- 25 mm in 14- days at room tcmperaturc (20 - 25C),
co nsistin g 01' a loose-tcxtured basal fclt, more or less lanate, very slightly
Iurrowcd and raised in the centre; with undulating margin largely submerged,
:xtending more than 10 mm beyond the aerial growth; heavily sporing in dccp
hlue-green shades (Sguy, PI. XXX, 447) : exudate and odour lacking;
colou red pigment diffusing into the surrounding ag ar not produced ; colo ny
reverse (colour plate 105, e) in yellow-orange shades; penicilli essentialIy as
described aboye but more consistentIy simple (appearing monoverticillate and
lending to ramigenous), with conidial columns more strongly accentuated than
tlpon the aboye substrata.
The species description is centered u pon the type strain CBS 216.28 = CM J
:~ 9 , 768 = ATCC 10,456 = IFa 5,764 = NRRL 909 = QM 7,587 = IJFM 7076,
isolated by K. Zaleski from soil in Poland, in 1927.
"
f( .
w..u..LJ.wJ
10 prn
@
@
Q B
} @
::o::(~
l'
100
jJm
c."R. .
extcnd ing about 2 mm or more beyond the a crial growrh; sporing thro ug ho u t
in gra y-grce n sha des (S gu y , PI. XXIX , 4:14); co lony S U r/i l CC pla nc, slightly
raised in the ce ntre ; ex udate lack ing or lirnitcd in amo un t, as minute d ro plets
en mes hcd in th e m ycelial fe1t; odour faint , ind cfinite ; rever se in bright yellowor ange sha de s (colour plat e 107 , c); golde n yellow -coloured pigm ent di ffusin g
into th e sur ro und ing agar; penicilli essentia lly as described aboye but with
conid iophores m ostly arising from th e subs tra tu m.
The species description is ce ntere d upon th e typ e strain IJ FM 7097 = ATCC
42 ,242 , and several other strains whi ch duplica te th e preceding in all
particul ars, all isolat ed by DI' A .T. M artnez from beech leal' litter in a beech
Iorest near N ancy, France, Septem be r, 1979.
Penicillium turolense is a d ist inc tive spec ies cha rac terized by its typi call y
div aricate peni cilli , with sho rt-po inted phiali des, by its spin ulose,
compara tively sm all and globose con id ia, and by its restricted gro wth upon C A
a nd M EA , a nd mor e rapid growth up on C YA. The correc t placement 01' thc
species , in our opin ion, appears to be m ost sa tisfacto r ily ass ignable to th e P.
canescens senes.
Colonies gro wing rather restrictedl y upon m ost subs tra te, cons isting usu ally
a close-textured ba sal felt with surface variable , consisting some times 0 1' a
network 0 1' tra ilin g hyphae 0 1' appearing m or e 0 1' less velve ty; con id ial areas
develop in g dull, 01'ten dark , gray to olive brown shades, almos t bl ack on so rne
su bstra ta; reverse typi cally develop in g deep orange -re d, very da rk brown , to
a lmos t black colou rs.
Conid iopho res vary ing gr eatl y in length and in origin, ar ising eithe r d irectl y
from th e substratum , 0 1' as short branches from ae rial hyphae.
Peni cilli typi cally strongly divaricat e a nd asym me tric, cons isting of
div ergent m etulae arisin g a t on e 01' more leveIs and bearin g clu sters of
ph ialides producin g conidial chains in loose colum n s 01' tangled ; sim ple
peni cilli (a ppearing mon overticillat e) are not infrequently encou ntere d.
Conid ia globose to su bglobose , stro ng ly ech in u late in sorn e species, smooth
a nearly so in othe rs.
0 1'
K ey to species
Page
l. Colonies not producing scierotia or masses of thick-walled cells. Penicilli
with the divaricate character well marked; growing rather restrictedly
upon most substrata; with metulae scattered on the conidiophore, or
usually aggregated into true verticils. Colonies developing typically dull
gray, dark brown, almost black shades (scarcely showing any green).
P. nigricans series .. .. 295
", ,1 ,
have been included in the cu rre nt study, namcly: Penicillium radulaium Sm ith,
P. granatense Ramrez, Berenguer et Martfnez , P. inflatum Stolk et Malla, P.
megasporum Orpurt et Fennell , P. ovetense Rarnrez et Martnez, and P. yarmokense
Baghdadi, all developing typi cal divaricate structures in sufficient numbers,
and dull, dark brown to almost black colours to necessitate pla cing the specics
here .
108 . Penicillium nigricans (Bainier) Thom : fig. 108, colour plate 108,
and SEM plate 18, (bottom: right).
0'.,' ~,,~ {)
C@C~~'"
f.6
(D O@
y~ y
L-........J
100pm
------
Penicillium nigricans (Bainier) Thom - 'The Penicillia' , pp. 351-353. 1930. See also
Raper and Thom, ' A Manual of the Penicillia', pp . 325-329. 1949.
Synonymies
Penicillium echinatum Dale - In Biourge's Monographie, La Cellule. 33: 278.
1923. discussed without name as C3 by E. Dale in Ann. Mycol. 12: 42. 1914; and
named by her in Ann . Mycol. 24: 137. 1926.
109. Penicillium albidum Sopp : Fig. 109, colour pIate 109, and SEM
w.wwu
10
jJm
~
0) ,_'i~..,1..,:,, @r7;}.
@ tS7 \:.~ ~1
'(
?0
~
re
. (-1
c ."
B ffft'i
<% "C,;f
.;;
~ ~~ ~
"2.1 f '~
;--~. \.. \\
Y.:fI
-::.:"';1
.~...,
'(.~:~!
t~
~
&' :
.
.m,
~\1
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l Y!)
~ l (~' yf
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100 prn
.' U 1
in uppcarance ; colony su rface overlaid by l thin, close ly wovcn nctw ork 01'
ae rial hypha e in fl uenc in g gre atly th e colony appcarancc ; hcavily spo ring
ihroughour in dark br own shade s, particul arly cons pic uo us in marginal areas,
a nd somc what ob scured in ce ntra l and subcentral areas by the den se net work
01' ae rial hyph ae sha di ng to wh ite rosy sha des; ex udate ab undantly produced as
yc llow-ora nge d rops ; odo u r lacking; no coloured pigment diffusin g into th e
surround ing agar; colony reverse (colour plat e 109, b) in re ddis h cinna mon
shades; penicilli esse ntially a s de scribed abo ye bu t with th e d ivaricat e cha rac ter
more strongly acce ntuated than upon the abo ve su bs tratum .
Colonies upon MEA (colour pla te 109 , C and c) attain ing a d ia mete r 01' 50
mm in 14 d ays a t room temperature (20 -25C), consisti ng 01' a looser tex tured
basal felt, th in , velve ty, cons pic uo us ly zon at e , not fur rowed , pla ne, wi th a very
ih in m argin mostly submerged, ex tend ing abou t 5 mm beyond the ae rial
gro wt h; heavily sporing through ou t in deep olive bro wn shades (Sguy, PI.
XX IX, 426 , 427); exuda te a nd odour lacki ng; no coloured pigm ent di ffusing
i nt o the su rround ing agar; colony revers e (colo u r pla te 109 , c) wit h redd ish
hrow n shades show ing th rough ; penicilli essentially as described aboye but
with coni dio p hores arising prim a rily from the su bstratu m, b eing longer, up to
;OO p.,m in len gth .
T he spec ies description is ce ntered upon strain CBS 254 .29 = CM I 40, 219 =
ATCC 10,408 = NRRL 2,034 = QM 7,48 1 = IJFM 7036 , isola ted by D . J anke
Irorn soil in 1928 , a nd m any ot her strains isolated fro m beech forest soil in the
Provin ce 01' Navarra, Sp ain , by D I'. A.T. M artnez , in 1977 .
110. Penicillium kapuscinskii Zaleski: fig. 110, colour plat e 110 , and SEM
S)~::~~ ~..
(ii
,b '(
~i"
t1I;
f}
I.--.J
100J,lm
tW
fR. .
i;~;~.
~r;;} \~~)
f2 r
1
l-,.-J
100 pm
'l Y
iR..
and radiately wrinkled sometimes in quadrants, with conidial area gray-green, deep
olive gray (Ridgway, PI. L1) at fi rst , becom ing drab in age (brownish drab , Ridgway,
PI. XLV) with mycelial hyphae , and marginal 3 mm white, showing stolon-Iike
hyphae reaching beyond the submerged mycelium and re-entering t he substratum;
hyphae very delicate ; reverse in pale orange shades; drops, small, colourless, well
distributed over the conidial area; conidiophores variable in length, arising from the
subst ratum or from aerial hyphae ; penicilli partly monoverticillate, mostly variously
showing one -branch, a group of sterigmata (phialides) around the main axis at the
first septum; branches (or metu lae?) 12-15 ,tm long; sterigmata (phialides) 8-10 ,tm
by 2.0-2.5 ,tm with conidial chains divergent; conidia 2.5-3.0 ,tm, with delicate
roughening or spinulosity seen only under oil immers ion .'
su r facc
and
m a rgin
plane ,
111. Penicillium melinii Thorn: fig. 111, colour pIate 111, and SEM
plat e 19, (centre : Ieft).
':1
"
"
"
:'
"
""
"
"
"
'0 0
"
10 jirn
"
o'
",,
,,
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f&)';\~
'&'
Ylr
e
.F?,
'-----J
100)Jm
Penicillium melinii Thom: In 'The Penicillia', p. 273. 1930; also Raper and Thorn,
'A Manual of the Penicillia', pp . 331-332 , 1949.
borne cithcr at thc apex 01' thc main axis 01' arising from lowcr nod es ;
conidiophores with walls strongly warty , sho rt , usu ally less than 100 Jtm in
Icn gth by 2.8-4.0 Jtm in diameter; metulae commonly 10-30 Jtm by 3.0 Jtm;
phialides comparatively large , in clusters al' 3-10 elements in the verticil,
mostly 9-15 Jtm by 3.0-5 .0 usu , conspicuously tapering to a long narrowcd
ncck, and tending to diverge; conidia globose, comparatively large, most1y
5- 6 Jtm in diameter, strongly echinulate , suggestive al' the chestnut fruit when
ob served under the light microscope (fig . 111, B) showing a strongly thorny
surface when observed under the SEM (plate 19, centre : left); con idial chains
111' to 100 tm in length, com monly divergent (fig . 111, C) .
Colonies upon CYA (colour plate 111, B and b) growing more rapidly than
a hoye, attaining a diameter 01' 40 mm in 14 days at room temperaturc
(20 - 25 C ) , similar in texture to thos e grown upon CA, but not abrupt1y
raised, and regularly sulcated by numerous radial furrows in subce ntral areas ,
with the centre irregularly folded in a more al' less cra te riform pattern, with a
(hin white margin, becoming light buff in age, surrounded by a narrow fringe
01' subm erged hyphae extending about 1 mm beyond the aerial growth; very
heavil y sporing throughout in dark olive brown shades, with the brown colour
pr edorninant , except in central areas whi ch remain persistently white;
cxuda te lacking; odour faint , 'mouldy'; no coloured pigment diffusing into the
surroun ding agar; colony reverse (colour plate 111 , b) apparently colourless
but with de ep brown shades showing through ; penicilli essentially as described
aboy e.
Colonies u pon MEA (colour pIate 111, C and c) att a ining a diameter 01' 50
mm in 14 days at room temperature (20-25C), consisting al' a looser textured
basal felt, appearing typically velv ety , not furrowed , plane , with centre
umbonate ; very thin margin largely submerged, exten ding about 5 mm al'
m ore beyond the aerial growth ; exuda te lacking; odour not pronounced ,
' mouldy '; no colou red pigment diffusing into the surrounding aga r ; colony
reverse (colour plate 111, e) in reddish brown shades with centre in deeper
brown colours; penicilli essentially as described above but with the stipes 01' the
conidiophores more coarsely roughened .
The spe cies descriptions is centered upon the type strain CBS 218.30 = CMI
40 ,216 = ATCC 10,469 = NRRL 2,041 = IFO 7,675 = QM 799 = IJFM 7053,
isolated by DI' Elias Melin from forest soil in 1929 in th e USA.
Penicillium melinii was assigned by Raper and Thom (1949) to th e
Asymmetrica-divaricata, sin ce an appreciable number 01' penicilli showed the
typi cal divaricate character 01' this group . Many , if not an actual majority , al'
th e penicilli , however, appear simple and a possible relationship to the
Monoverticillat a should not be overlooked . However, the globose, strongly
echinulate conidia and th e production al' deep brown colours with the ripening
a l' con idia are strikin gly suggestive al' th e Penicillium nigricans series , and
parti cula rly 01' P. nigricans itself lt dilfers I'I'O Ill P. nigricaus in its slro ng ly warty
co nid iop ho rcs .
112. Penicillium raciborskii Zaleski: fig. 11 2, colour plate 112 , and SEM
4b"c~
r.l o
:
'
~l ~:~! ~ .
L - -jrrn
-J
100
"
Penici/lium raciborskii Zaleski - Bull. Acad. Polonaise Sci . Math. Nat. Sr. B, pp.
454-455.1927 . See also Thom , 'The Penicillia', pp. 318-319.1930 ; also Raper and
Thom, ' A Manual of the Penicillia', pp . 333-334. 1949.
Coloni es upon CA (co lour p lat e 112 , A and a) attaini ng a di ameter 01' 45 m m
in 14 days at room tempcrature (20 - 25 C) , consistin g 01' a clos e-textured basal
felt overlaid by a dense , clos ely wov en network 01' aerial hyphae lend ing a
la na te appearance to th e colon y surface , stron gly furrowed in an irregular
pattern , rem aining pe rsi stently white th rou ghou t , w ith th e exce p tion 01' the
inter-colony margins where con id ial structures a re a bu ndan tly produ ced in
vcry pale gray-grcen sh ades (Sguy, P I. XXX , 44 5) ; exu da te lim ited in
I' he spe cies dcscript ion is ee nte re d upon the typ e strain c ns 224. 28 - e M I
40 ,56B - .vrc o 1O ,4B8 = NRR L 2,150 - I Fa 7,67 6 - IJ FM 7052, isolat ed by K.
Zaleski from forest soil under eonifc rs, in Pola nd , in 1927. 1t is kn own only
from th e typ e strain.
113 . Penicillium radulatum Smith: fig. 113 , colou r plate 113, and SEM
plat e 19 , (bottom : left) .
<.\
..
'.~
~?~
. '
- B()
.\:':
'" ~~ (\).!1r1.'
. \':'0::0;;: f'
~ . .....
r
.
V6
\ ~
'1
100 jrrn
iR.
'(~
Penici/lium radulatum Smith - Trans . Brit. Myeol. Soe. 40: 484-485. 1957. See also
M.M. Kulik, 'A Compilation of Deseriptions of New Penici/lium Speeies' . Agrie .
Handbook No. 351. Agrie. Res. Ser . U.S. Dep . Agrie . pp. 68-69. 1968.
Smith's original diagnosis follows, sinee this adequately presents the species in
question when first isolated :
' Colonies on Czapek agar spreading broadly, circular with regular margins, dark
bluish green with narrow paler edge during the rapidly growing period, beeoming
very dark smoky blue -green , smooth , velvety; respiration drops (exudate) minute,
yollow; reverse somewhat greenish yellow, with the colour diffusing throughout the
r; on malt agar or potato agar spreading fairly rapidly, 23-30 mm in 9 days at
/loe , dark grayish blue -green with paler bluish margin, velvety; drops minute,
yollow; reverse yellow to orange, with the colour diffusing slightly; penicilli
mnrkedly divaricate with the branches loosely divergent, usually with a terminal
luster of metulae and phialides mixed, and with a series of branches from lower
nodes, bearing metulae and phialides or phialides alone; conidiophores arising from
tho substrate, very rough, fairly long and 2.2-2.5 .tm in diameter; rami and metulae
ou on indistinguishable and very variable in length, rough; phialides rough, fairly
ihruptlv pointed, 8-9 by 2-2.4 .tm; conidia globose, spinulose, mostly 2.8-3.2 .tm
111 diameter, but occasionally up to 4.5 .tm, in chains which are usually divergent at
Ilrst, becoming tangled, but are sometimes more or less parallel or even in loosely
olumnar rnasses.'
O u r notes follow :
C olonies upon CA (colour plat e 113, A a nd a) growing rather restrictedl y ,
Illaining a diameter of 35 mm in 14 days at room temperature (20-25 C) ,
I'I lIIs isting of a close-textu red basal felt , typi cally velvety, not furrowed , plane ,
with thin undulating m argin su rrou nded by a fringe of submerged hyphae
xtc n d in g ab out 1 mm be yond th e aerial gr owth ; heavily sporing throughout in
d ilfcrent shades of blue-gr een, depending on th e degree of rip eness of conid ia ,
IlCing darker in m arginal area s (Sgu y , PI. XXX, 447) ; exudat e abundantly
produced , particul arly in th e colony cen tre, as deep or ange-coloured dropl ets;
odo ur lacking; deep yellow-oran ge-c oloure d pi gm ent diffusin g into th e
surr ounding agar ; colony reverse (colour pIat e 113 , a ) in or ange shades;
conidial structu res essentia lly as des cribed aboye (Smith 's original diagnosis)
( Iig. 113, A) , with conid iophores cons picuo us ly warty and phialides definitely
rou gh; con id ia are strongly rou ghened, appearing spinulose wh en obs erved
u nd er th e light microscop e (fig. 113, B) but th ey show stro ng echin ula tion
whe n ob serv ed under th e SEM (pla te 19, bottom : left) , mostly 3. 0 - 4 .0 /lm in
di ameter: conidial chains are loosely div ergent (fig . 113, C ).
C olonies upon CYA (colour plate 113, B a nd b) growing m or e rapidly th an
I he preceding, a ttaining a diamet er of 50 mm in 14 days at room temperature
(2- 25 C ), con sisting of a close -tex ture d basal felt , velvet y, with m argin
plane , not furrowed , but with cen tra l area deeply radiately furrowed and
slightly rais ed , cons picuo us ly zonate; medium spo r ing in pale drab sha de s
(S gu y , PI. XL, 590); exudate abu nda ntly produced as deep reddish or angecoloured large drops ; odour lacking; reddish brown-coloured pigm ent d iffusin g
i nto th e su rro und ing a ga r ; colony revers e (colou r plate 113 , b) in deep redhr own shades; penicilli esse ntially as described abo ye .
C olonies u pon M EA (colour plat e 113, C a nd c) spreading broadly,
a ttain ing a di ameter of 50 mm in 14 days a t room temperature (20-25 C),
cons isting 0 1' a looser tcxtu red busal Iclt , vclv ct y , plnn c , more 0 1' less zo na te in
m argin al a rca, not Iurrow ed , wi th thin m a rg in surrounclcd by l wid c frin ge 0 1'
su b merged hyphae extendi ng 5 m m 0 1' m ore bcyond the aer ial gro wt h; ve ry
h eavily spo r ing throu gh ou t in ve ry dark gr cen , almost b lac k shadcs (Sgu y , PI.
XXX , 44 6); exu date a nd odo ur la ckin g 0 1' faint , a ro matic; no colou rcd
pi gment diffusing into th e surro un d in g agar; colony reverse (colour platc 113 ,
c) in yellowish shades with a green ca st show ing throu gh ; penicilli essen tially as
described aboye .
The species description is ce ntere d upon the ty pe strain CBS 250 .58 = C M I
71 ,624 = ATCC 18 ,383 = Q M 7, 526 = IJFM 7039 , isolat ed by M r G .W .Sewe ll,
01' th e R oyal H ollow ay College , from Calluna hea thl and soil from Puttenharn
Com mo n , Su r rey, U K, M arch , 1954 . Several strains 01' Penicillium radulatum
we re recove red by G. W .Sewell from hea thl and soils in N ewb or ough ,
A n glesey, in Godlingsto n H eath a n d Pl at eau H eath , D orset , in K ell in g H eath ,
Norfolk, a nd in Rhossilli D owns, Gl amorganshi re , Wales.
Sp ecies 0 1' Penicillium in wh ich b oth co n id iophores an d phialides are
d efinitely rou gh a re few in number; a mo ng them : P. argillaceum St olk , Evans el
Nil sson , P. pallidum Smith, P. putterilli T hom , a n d P. namy slowskii Zaleski have
smooth wall ed con id ia , but no other spe cies is kn own so far , except P. radulatum
Smith , h a ving all pa rt s of the penicillus , in c1uding th e phialides, as well as th e
co n id ia, co nsp icuo us ly as pe ru la te .
:11 I
~
10 prn
{1
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0} ;:J.. 4y:.;.
'-';.
"
;, {[l
'; B
..>.;.
"''''
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" ,"
~
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' " iR,
.
~.,
100 prn
-;'
'
whit e hyph ae partiall y obsc uring th e co nid ia l struc tu res, es pcc ia lly in the
ce n tre; cx udate a bun d a ntly produ ced , as uncoloured, crys tal c1ear drops;
odo ur fa int , indefinite, 0 1' la cking; very pale rcddish br own pigment diffusing
into th e surrou nd ing a ga r; co lony revers e (colour plate 114 , b) in pale rcddish
cin namon shade s; penicil1i esse ntially as described aboye.
C olonies upon M EA (co lour plat e 114 , C and c) gro wing restrictedly,
attain ing a di ameter of 35 mm in 14 days at room temperature (20 - 25 C ),
co ns isting of a looser textured bas al felt , not furrow ed , plane , typically velvety,
with white , finel y cre ne lla ted m argin with a frin ge of submerged h yphac
exte nd ing a bo u t 2 mm beyond th e ae rial gro wth ; very heavily spo ring
th rou gh out in very dark blue-brown, almost black shade s (S guy , PI. XXXIV,
506) ; no colou re d pi gm ent diffusing into th e surrounding agar; colon y reverse
(colour plate 114 , c) developing reddish brown shades; exudate la cking; odou r
aromatic ; penicilli esse n tially as described a boye .
The species description is ce nte re d upon th e typ e stra in IJFM 5965 = ATCC
42,233 , isolated b y th e author from th e a ir of Madrid , Spain , Apri11979 . It is
ac tually kn own only from th e typ e strain .
Penicillium granatense is a distinctive species c1e arly characte rized b y its
strongly di varicate pen icilli , th e stro ngly ech in ula te conid ia, and by the very
d ark, almost black colou rs develop ed b y th e colon ies gro w n on MEA,
suggestiv e of th e P. nigricans se ries, to which th e species appears to be most
sa tisfacto rily a ssignable.
115. Penicillium inflatum Stolk et Malla: fig. 115, colour plate 115, and
/ J .I
Lu.&.J.J.=J
10pm
l5SJ rp
(j)
@ @~ <b
O-sa-fl5rj;
fd
-@
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.
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L...-....l
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';~
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arranged, glving the peni cillus and Asporgillus-lIke appearance; also reduc ed forms
consisting of only two metulae and monoverticillate penicilli occur; all elements of
the penicillus are hyaline and smooth-walled . Metulae developing successively on
the vesicula-like apex of the conidiophore, occasionally occurring also on its
subterminal portion; c1ub-shaped, rarely branched, 5-10 ,tm long, narrow at the
base, 1.5-2.2 ,tm in diameter, broadening gradually to the swollen apex, 3-6 ,tm
diameter. Phialides developing successively on the swollen apex, occasionally also
on the subterminal portion of the metula , occurring in small c1usters of 3-8, slightly
diverging, 5.5-7.5 ,tm by 2-3 ,tm, less wide at the base, narrowing at the top
abruptly to a small, but definite conidium-bearing tip (neck) (0.5-1 ,tm long).
Conidia brownish, globose to subglobose, slightly roughened, provided with mostly
two roughly parallel, very thin bands, 1.7-2.5 ,tm in diameter. Conidial chains
diverging, somewhat tangled, short, up to 60 ,tm long.
Minimum temperature 5C , optimum temperature 20-25C, maximum
temperature 30C .
Because of the swollen apices of the conidiophores and its many strongly
diverging metulae, which in large heads may be radiate, the habit of this new
species suggests a species of Aspergillus. It differs, however, from this genus in
some important characters. No defin ite foot-cells are present, the metutae do not
develop simultaneously on the apex of the conidiophore as is known to be the case
in true Aspergillus, but they develop successively. Moreover the conidiophore wall
is thin, whereas most Aspergillus have thick walls. The species shows much closer
affinities to the genus Penicillum, especially to the P. nigricans series. Its cultures
are grayish to olivaceous-buff like in this series, the conidia are brownish coloured,
while the structure of the colony agrees completely with P. nigricans (Bainier)
Thom.'
The origina l diagn osis presented aboye esse n tially con forms with our own
ob servations of th e typ e strain in con nection with th e present study. Our
additional cult ural observatio ns follow:
C olon ies upon CA (co lour piate 115, A and a) growing ve ry restrictedl y,
attaining a diameter of 10 mm in 14 days a t roo m temperature (20 -25C),
co nsisting of a tough , cio se-t extured ba sal felt , a ppearing velv et y , ove rla id by a
ciosely woven network of white vegetative h yphae influencing greatly th e colony
appearance , colony surface strongly convex, abo ut 3 mm deep ; ve ry slightly
spo ring, remaining persistently whi te; ex udate lacking; odo ur non e; no
colou red pigment diffu sin g into th e surroundin g a ga r; colony reverse (colou r
plat e 115, a ) in pinkish cin nam on shades .
Colonies upon CYA (co lour plate 115, B and b) growing more rapidly th an
upon C A, att ai n ing a di amet er of 25 mm in 14 days a t roo m temper ature
(20 - 25C) , cons isting of a close-texture d basal felt , ap pearing deepl y velvety
to lanate , stro ng ly rad iately wrinkled and grad ually rai sed tow ards the
J I ",}
nct work ol' white vegetati vo hyphae, inlluen cin g thc colony appe a ra nce a nd
OI.lsl:lII'ing com pletcly th e otherwise medium spor ing areas, whi ch remain
pcrsis ic ntly sno w white in colou r; exuda re abundantly produced as minute ,
crystul clear, colourless droplcts enmes he d in th e m yceli al felt ; odo ur lacking ;
pille arnber-colou re d pigment d iffus ing into th e surro und ing agar ; colony
n -verse in ye llow-cinnamo n shades (colour plat e 115 , b).
C olon ies upon MEA (colour plate 115, C and e) attaini ng a diameter 0 1' 30
111 111 in 14 days a t room temperature (20-25 C) , cons isting 0 1' looser textured
hnsal felt , not furrowed , thin, plane , with cen tre umbonate , cons picuo usly
zu narc : very thin m argin largeIy su bme rge d, ex te nd ing abo u t 10 mm beyond
11)(: ac rial growth; spo ring abundantly in pale brown to buff shades (Sguy,
X I,VII, 705); exuda te and odour lacking; no coloured pigment di ffusin g int o
1111: surro u nd ing agar; colony revers e (colour pia te 115, e) in ye llow ish b rown
ru lours ; penicilli esse ntially as described in th e origi na l d iagn osis (fig. 115 , A
und C); conid ia (fig. 115 , B) essentially a s described aboye whe n obse rve d
uu cle r th e light m icros cop e , but showing well-d efin ed spirally ridges wh en
oh scrved under th e SEM (pla te 20 , top : left) .
The species description is centered upon th e typ e stra in C BS 682 .70 = IJFM
7()(j2, isolat ed with four addi tional strains from the root surface 0 1' Picea excelsa
Link , in th e L venholm di strict , ]utland , by D .S . M alla , Sta tens For stli ge
I'orsgsva esen , Springforbi , D enmark , in 1970 , a nd in the H rsh olm di strict
in the northern part 01' Sjaelland , D enmark , in 1969 . An add itional stra in wa s
ixolated by R.A . Sa mso n, C .B. S. , Ba arn , The N etherlands, from forest soil
lindel' Quercus robur L. , in Spaande rswo ud nea r Hilversum , The N etherl ands ,
in O ctober 1970 .
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blaek (Ridgway, PI. XLVII) due to the presenee of abundant eonidial struetures,
shading into a white submarginal area; exudate laeking; odour not distinetive;
reverse olive-oehre to yellow-olive at 10 days, near Prout's brown (Ridgway, PI. XV)
in age, with the pigment diffusing into the agar; eonidiophores arising from the substrate or the aerial felt, septate, smooth, hyaline, 3.0-4.5 .m in diameter and up to
300 .m in height; penicilli biverticillate, asymmetrie, divarieate, variable in pattern
but usually eonsisting of a verticil of 2-6 divarieate metulae; mostly 4.0 by 10-12
.m; phialides 3-5 per metula, divergent, bottle shaped, 5.0-7.0 by 3.0-4.0 .m;
eonidia in short divergent ehains, spherieal, averaging 6.7 .m in diameter (5.0-7.0
.m), thiek walled, dark olive-brown at maturity, eoarsely eehinulate, with dark eoloured spines 1.0-1.5 .m in length; prominent disjunetors 1.0-2.0 .m long by
1.0-1.5 .m wide.
Colonies on malt extraet agar growing more slowly, attaining a diameter of 35-40
mm in 10 days, flat, unfurrowed, vegetative myeelium submerged, not forming a
brittle felt, heavily sporulating, abundantly velvety but showing a thin surfaee network of aerial hyphae bearing seattered conidial structures, with thin marginal area
white, shading rapidly through shades of American green to dull blackish green
(Ridgway, PI. XU, finally becoming olivaceous black (Ridgway, PI. XLVII); no
exudate; reverse uneoloured or nearly so; penicilli as deseribed above but oeeasio-
nolly showing much more complex structures rebranched as many as five or six
times below the level of the phialides, consistently producing the coarsely
hinulate, spherical conidia characteristic of the species.'
' I'he original diagnosis presented aboye essentially conforms with our own
nhscrva tion of the type strain in connection with the current study . Our own,
ud d ition a l, cultural observations follow :
C olonies upon CA (colour plate 116, A and a) attaining a diameter of25 mm
iu 14 days at room temperature (20- 25C), consisting of thin, close-textured
husal felt, not furrowed, flat , with thin margin surrounded by a narrow fringe
111' submerged hyphae extending about 1 mm beyond the aerial growth, colony
xu rl ace persistently white in marginal areas and showing pale yellow shades in
submarginal and central areas ; almost sterile , with colony surface overlaid by a
I hin, closely woven network of vegetative hyphae ; exudate and odour lacking;
ura ngc-colou red pigment diffusing into the surrounding agar; colony reverse
i 11 pal e yellowish shades (colour plate 116, a) .
Colon ies upon CYA (colour plate 116, B and b) growing more rapidly,
nu a ining a diameter of 40 mm at room temperature (20-25C) , similar in
tcxture to the preceding, but with undulating white margin slightly furrowed,
surr ounded by a fringe of submerged hyphae extending about 1 mm beyond
1he aerial growth; colony surfac e very poorly sporing, usually with conidial
a rcas obscured by a dense network of aerial vegetative hyphae lending a pale
llrange colour to the colony , which is almost plane and not furrowed in central
a nd submarginal areas; orange coloured pigment diffusing into the
surrounding agar; colony reverse sim ilarly coloured (colour plate 116, b) .
Colonies upon MEA (colour plate 116, C and c) attaining a diameter of 25
111m in 14 days at room temperature (20-25 C) , appearing 'wet' , white,
slightly raised in the centre , not furrowed ; slowly shading towards deep olive
hrown colours, becoming almost black with the ripening of conidia after 21
days (colour plate 116 , C); heavily sporing throughout ; colony surface overlaid
by a thin network of tloccose vegetative hyphae and ropes of hyphae
intluencing the colony appea rance during the first two weeks, then being
obscured by the ripening of conidia ; colony reverse in pale yellow shades
(colour plate 116 , c).
Penicilli essentially as described in the original diagnosis (fig . 116, A), and
conid ia attaining a diameter of 7 tui, conspicuously echinulate (fig . 116, B and
SE M plate 20, top : right).
The species description is centered upon the type strain CBS 256 .55 = ATCC
12,322 = NRRL 2,232 = QM 6,879 = WB 2,232 = IJFM 7046, isolated by] . H .
Warcup from heathland soil in Suffolk, UK, in 1947. Similar strains have been
isolated by P .A. Orpurt and D .!. Fennell from grassland soil collected at a
depth of one inch at five stations in southern Wisconsin , USA . between May
195 2 and M ay 19:>3. 0 1' th e nin c strai ns isolat ed Ih >111 Wis consin soils , cight
duplicated th e pattern presented by the typc . Howevcr, une isolat e , d iffered
fro m th ese stra ins in devel oping more slowly-grow ing, deep er , almos t lan at e
colo nies; in produ cing m ore variable and o fte n fractional or m al formed
penicil!i; and , in bearing ' wa r ty' rather th an stro ng ly ec hin ula te co nid ia .
Stra in C B S 529 .69 = VK M F-l ,824 wa s isolated by G .C. Blatt from forest soil
under Thuja occidentalis, in Canada , 1969 . Strain C BS 121. 65 was isolat ed by J.
Nicot from beach sa nd , in th e Leb anon , in 1965 .
Penicillium megasporum appears most satisfact orily relat ed to sp ecies of th e P.
nigricans se ries, espec ia lly to P. melinii, altho ugh it differs m arkedly from th e
latter sp ecies in cer ta in striking fea tures , namely : The globo se, large, rough
co nid ia of P. megasporum exc eed ing 6.5 Ilm in di amet er, wh ereas th ose of P.
melinii m easure 3.0-3 .5 uui ; a nd th e co n id iopho res of P. megasporum are
smooth upon al! substrata while th ose o f P. melinii a re cons picuo us ly warty .
T he sp ecies m ay be rega rd ed as a soil org a nism world-wide in d istribution .
117 . Penicillium ovetense Ramrez et M artnez: Fig. 117 , colou r plat e 117,
and SEM plate 20 , (centre: left).
~~~Jr
1:-- t
100l'm
f.R-
hyphae extend ing abou t 2 mm beyond the aerial gro wt h; colony surf ace fla t,
not furrowed ; heavil y sporing throu gh out in dcep olivc-gray shades (S guy, PI.
XXIX , 433 ); exuda tc and odour lacking; no colou red pigm en t diffusin g into
the su rro und ing ag ar; colony reverse (colour plate 117, e) brown with a gr een
cas t show ing throu gh . Penicilli essentially as described aboye .
The spec ies description is ce ntere d upon th e type stra in IJFM 7029 = AT CC
42,237 , isolat ed frorn sandy soil in M adrid , Spain , in 1979 , by th e author. It is
at present known only from the typ e strain .
Penicillium ovetense is a di stinctive species characterized by its typ ical
divaricate penicilli, although sim ple forms are not unfrequ ently enco untered
(appearing monoverticillate), by its globose, spin ulose conid ia , by its restricted
gro wth on most su bstra ta, a nd by th e ve ry dark olive-gray shade s a l' its colon ies
u pon MEA , su ggestiv e a l' th e species inclu de d in the P. nigricans seri es to which
it appea rs mo st satisfactor ily ass ignable .
118 . Penicillium yarmokense Baghdadi : fig. 118, colour plat e 118, and
y:~
1.....----1
100)Jm
nlcillium vermokense Baghdadi - Nov. Syst. Nlz. Rast. pp. 99-101. 1968.
~ ol o n i e s
0 0
08 00
0 0
O
co
00
o o 0 00
o 00 O o
00 o
o o
R.
22 to 24C , tanate , grayish-green , with radial zones, spotted in violet red shades ;
colony reverse and agar similarly coloured in deep red shades approximating ox
blood (Sguy, PI. X, 141); exudate Iimited in amount, colourless or lacking .
Conidiophores arising from aerial hyphae, 25 to 150 Ilm in length, smooth-walled.
Penicilli irregular in pattern, mostly divaricate, consisting of c1usters of 2 to 4
metulae, although simple penicilli (appearing monoverticillatel and irregu larly
branched are not unfrequently encountered. Branches, 8 to 15 1lm by 2.0 to 2.5Ilm;
metulae, 6 to 12 1lm by 2.0 to 2.5Ilm; phialides bottle-shaped, 5.0 to 7.5 1lm by 1.7
to 2.2 Ilm , in c1usters of 5 to 8 elements in the verticil , sometimes single on the t ip of
the metula. Conidia globose, varying in dimens ions , 1.5 to 1.8 1lm or 3.0 to 3.5 1lm in
diameter, smooth-walled , in short diverging chains.' (Translated from the French by
the author) .
01' floccosc aerial hyphae in lucn cin g gre a tly (he colo ny a ppcara ncc , not
furrowed, slightly and gradually rai sed towards the centre ; thin evcn margin
surrounded by a submerged fringe extending about 1 mm bcyond the aerial
growth; heavily sporing throughout in dark blue-grcen shades (Sguy, PI.
XXX, 447) in sectors not ov erlaid by the nctwork 01' floccose hyphae, 01'
showing arte misia gre en shades in zones covered by th e m ention ed network 01'
vegetative h yphae; odour lacking; exudate abundantIy produced as minute,
crystal clear droplets enm eshe d in the my celial felt; bright yellow-orange
coloured pigment diffusing into the surrounding agar; colony reverse (colour
plate 119, a) developing bright yellow colour; penicilli (fig. 119 , A) typi cally
div aricate and asymmetrical, with con id iophore s smooth walled, arising from
a erial hyphae, up to 200 Jlm in length by 2.8-3 .0 Jlm in diameter, consisting 01'
branches variously arranged along the conid iophore, and 01' clusters 01' metulae
01' 2-4 elements in th e verticil, bearing cro wded whorls 01' phialides; branches
usu ally ranging from 10 to 30 Jlm in length by 2.5-3.0 tut: in diameter;
mctulae , 8-15 Jlm by 2.5-2 .8 uiu, with a pices slightIy enla rged in a vesicle up
to 3.5 Jlm in diameter; phialides bottIe shaped , in clusters 01' 3-6 elements in
th e verticil , div ergent , mostIy 7-8 Jlm by 3.0 ust , conspicuously tapering to a
short , narrowed ne ck ; conidia glob ose, smooth wall ed wh en obs erved under
the light microscop e (fig . 119 , B and C), but finel y rough ened when obs erved
under th e SEM (plate 20, bottom: left) , ranging from very small, 1.5-2 .0 Jlm
diameter (fig. 119, C) to larger, about 3 .0 Jlm in diameter (fig . 119 , B) ;
conidial cha ins producing short div ergent colu m ns (fig . 119, D).
C olonies upon CYA (colour plate 119 , B and b), attaining a diameter 01' 45
mm in 14 days at room temperature (20-25 C) , cons isting 01' a close-textured
basal felt overlaid by a looser-textured network 01' floccose hyphae lending a
lana te appearance to th e otherwise deeply velvety colony surface, whi ch is
deeply radiately furrow ed with th e centre irregularly foldcd and depressed ;
thin white m argin surrounded by a fringe 01' su bm erged hyphae extending
about 1 mm beyond th e aerial growth; conspicuously zanate owing to the
variou s degrees 01' ripeness 01' con id ial zones ; heavily sporing through out in the
same shades a s th e preceding, with conidial areas more 01' less obscured by the
deep floccose ov er growth 01' aerial hyphae ; exudate abundantIy produced , a s
minute yellow droplets enmeshed in th e mycelial felt ; odour lacking; am bercolou re d pi gment diffu sing into the surround in g agar; colony rcvers e (colour
plat e 119 , b) developing reddish cinnamon shades ; penicilli essentially as
des cribed aboye.
Colonies upon MEA (colour plate 119 , C and e) attaining a diameter 01' 35
mm in 14 days a t room temperature (20 -25 C) , consisting ofa looser tcxtured
basal feIt, deeply velvety , overlaid by a loo sely wov en network 01' floccose aerial
hyphae lending a lanat e appearance to th e colony surface , not furrowed ,
slightIy rais ed tow ards th e centre , which is m arked by a loose overgrowth 01'
,J';;
loccul cn t hyphae; heavily spo r ing th rou gh out in oliv e-gr een shade s (S guy,
PI. XXIX, 422), partially obsc u rc d by the Iloccose ove rgrowt h 01' aer ial hyphae
whi ch dev elop blue- gr een sh ades (Sguy, PI. XXIX, 425) ; odo u r and exudare
lacking; deep gr enat e , blo od red, to deepl y vinaccou s, beautifully coloured,
pigment diffusing into th e su r ro u nd in g agar; co lony reverse (co lour plate 119,
e) dc veloping deep purple red to blood red colou rs; penicilli essen tially as
d escribed above.
The sp ecies description is ce n te re d upon th e type st rain C ES 38 0 .75 = C M I
197,488 = IJFM 7058 , isolat ed b y DI' Bui xu a n D ong , Faculty 01' Pharmacy,
U niv ersity 0 1' H anoi, Vi etnam , from see ds 0 1' Trit icum vulgare L. in a cereal
preservin g pit , Prague , Czechoslovakia, April , 1971.
Penicillium atro-sanguineum is a ve ry distinctive sp ecies characte rized
particul arly by th e deep red pi gment develop ed upon MEA a nd diffusing into
t he su rrou nd ing a ga r , by its typicall y divaricate penicill i produ cin g co nid ia 0 1'
two di ffer ent sizes , eithe r in th e sa me penicillus 0 1' in different st r uc tures,
whi ch is one 01' th e most striking features 0 1' thi s organism o
Ir ap pe a rs to be m ost sa tisfac to r ily assi gn able to th e Asymmetri ca-divaricata sub-section , bu t it ca nno t be placed in a ny 0 1' R ap er a nd
Thom ' s (1949) recognized series , hen ce its inclusion here .
120. Penicillium griseo-purpureum Smith: Fig. 120, Colour plat e 120, and
SEM plate 20, (bottom right).
Penicil/ium griseo-purpureum Smith - Trans . Brit. Mycol. SOCo 48: 274-275. 1965.
See also M.M. Kulik, 'A Compilation of Descriptions of New Penicil/ium Species' .
Agric. Handbook No. 351. Agric. Res. Ser. U.S . Dep. Agric. p. 43. 1968.
:1 ~ 1>
O~o
0
0 0 O
00 B O
O O 0000
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. J/
'
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c ;'
.'
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L-----I
100,JJm
l'
fR. ,
1\), with co n id io p ho res sm oo th wall cd 01' ncarl y SO, sc p ta tc, a r ising' di rectl y
Iro m the su bs tru tu m, c nla rgc d a l th e a pex in a vesicle u p lo :> un in di um et cr ,
usu all y up l o 1:>0 un in lcn gth by 2.0 - 2 .:> J.l1II in diamct er , bcaring who rls 01'
mctuluc a r mare a l' lcss unequal len gth , 10- 2:> J.l1II by 2.0 -3.0 iutv , enla rge d a t
th e a pex in a vcs icle u p to 4.5 J.lm in dia m ct cr ; phialides in clu sters 01' 3 -8
clc mc n ts in th e ve r ticil, bottl e shaped , m ostly 10 iut: by 2.5 -3 .0 uin ,
conspicuously tapering to a shor t narrow ed neck ; co n id ia com para tively small,
xmooth wall ed 01' nearl y so wh en obse rve d under th e ligh t m icr oscop e (fig. 120 ,
B) but delica tely gra n ula r wh en obse rve d under th e SEM (pla te 20, bottom :
rigbt) , mostl y 2 .0 -2 .5 J.lm in lon g ax is; co nid ia l chains sho rt, in loose colu m ns
( Iig. 120 , C) .
Colonies u po n C YA (co lour pl at e 120 , B a nd b) gr owing more rapidly than
ahove, a ttain ing a di am et er 01' 35 mm in 14 d ays at roo m temperature,
co nsisting 01' a close -textu re d basal felt , typicall y a nd fin ely velvety,
cons picuo us ly za nate owi ng to the various degr ees 01' ripe ness 01' co n id ial
structures; m argin Ila t, not furrowed , su rrou nde d by a narrow fringe 01'
subme rge d h yphae ex te nd ing a bo u t 1 m m beyond th e ae r ia l growth;
su bm arg inal a rea a nd cen tre d eepl y radia tely fu r ro wed with ce n tre irregularly
olde d in a crater ifor m pattern a nd sho wing crev ices; heavil y spo ring
rhroughout d evel oping brownish gree n sh ades , becoming blue- gr een with the
ripe ning 01' co n idia (Sguy, PI. XXXI , 453) , sta r ting in m arginal a reas;
cxudate a b u ndan tly produced as large drops developing deep vinaceous red
colou rs; odo u r lacking; deep red -colou red pi gm en t diffusin g into the
su r rou nd ing agar; colony reverse (colour plate 120 , b) in the same d ark violet
pu rple show n on C A; th e agar being stron gly pulled away fro m the Petri d ish ;
penicilli esse n tia lly as described .
C olonies upon M EA (colour plate 120 , C a nd e), sp read ing broadly ,
a ttain ing a diameter 01' 50 mm in 14 da ys at ro om temperature (20 -25 C),
cons isting 01' a looser textured basal felt , typi call y velvet y , flat , not furrowed ,
with thin m argin su rrou nde d by a wide frin ge 01' su bme rge d hypha e exte nd ing
abou t 5 mm beyond th e ae r ia l growth; heavily sporing throu gh out in graygree n shade s (Sguy, PI. XXI , 309); ex udate a nd odour la cking; no coloured
pigm ent di ffusin g into th e surro u ndi ng agar; colony revers e (colour plat e 120 ,
c) developing reddish brown shades sta rti ng at th e ce ntre a nd exte nd ing
gra dually towards the m argin ; penicilli esse n tia lly as described a boye .
The spec ies description is ce nte re d upon th e typ e strain C BS 406 .65 = C M !
96 ,1 57 = A TCC 22 ,353 = !J FM 7042, isolat ed by B.A . Indraratne from forest
soil under Pinus sp ., Freshfield Forest , Lancaste r, UK , 1962.
Penicillium griseo-p urp ureum clearly belon gs to th e Asym me trica - d ivarica ta
su b-section, a nd is distinguish ed by th e deep violet -purple colou rs sho w n by
th e colony reverse upon CA a nd CYA , by th e deep red-coloured pi gm ent
di ffusin g into th e surrou nd ing agar upon C YA , a nd by its co m parative ly sm all
:1:U
conid ia . Howcver , it cannot be sutis fuctor ily assigned lo any 01' the Raper and
T hom's ( 1949) recogn ized se ries, hen ce its in clu sion here. lt is actually know n
only from the type st ra in .
T he Penicillium brasilianum series is another newly erected group 01' Peni cillia ,
admitted artificially in concept, wh ich has bee n designed to cover five for ms
wh ich cannot otherwise be satisfactorily placed in any 01' R a per and T hom' s
(1949) recognized series, namely : Penicillium brasilianum Ba tista , P. kabunicum
Raghdadi, P. moldavicum Milko et Belja kova, P. onobense R am rez et Martnez,
and P. castellonense R am rez et Martnez. A close re lationship be tween these
species is, however , not necessarily presumed, and they are considered
together primarily as a matter 01' conven ience. T hey do, however, show certain
characteristics in common, particularly the divaricate character 01' th e penicilli,
its broadly spreading co lonies upon most substrata, the deeply floccose felt 01'
the colony surface, and its us ually strongly ellip itical, more or less apiculate
and rough conidia.
Key to species
Page
l. Colonies not producing sclerotia or masses of thick-walled cells.
Colonies showing some shades of green, gray , gray green, or blue-green
with the ripening of conid ia. Penicilli with the divaricate character
evident. Colon ies spreading broadly upon all substra ta, appearing more
or less floccose.
P. brasilianum series
A . Conidia globose or subglobose .
1. Conidia conspicuously spinulose.
P. castellonense Ramrez et Ma rtnez
2. Conidia smooth-walled or nearly so, pyriform , w ith one apiculate
end .
P. kabunicum Baghdad i
B. Conidia Iimoniform , conspicuously spinulose.
P. moldavicum Milko et Beljakova
. ... 328
... . 338
. . .. 331
. .. . 334
121. Penicillium brasilianum Batista: fig. 121, colour plate 121 , and SEM
plate 21, (top: left).
.'
;:.
o@ BW'@
JJ
~~ : 8~
Lu.uJ.J.wJ rl'<l
10pm
ti"
i l rt~at.
Penicillium brasilianum Batista - Ann. Soco Biol. Pernambuco. 15: 160. 1957. See
also M.M. Kulik, 'A Compilation of Descriptions of New Penicillium Species'.
Agric. Handbook No. 351. Agric . Res. Ser. U.S . Dep. Agric. p. 21. 1968.
Batista's original diagnosis follows:
'Colonies upon neutral Czapek's agar attaining a diameter of 35-40 mm in 10
days at room temperature, consisting of a thin f elt, gray-green, velvety, superfic ially
f loccose, wrinkled , plane, sometimes umbonate, with radial furrows, w ith even
margin, 2-10 mm wide; at first, after 3 days old , they are white; azonate; odour and
exudate lacking ; colony reverse yellow, appearing orange-yellow in some stra ins,
soon becoming dark brown .
Vegetative submerged hyphae hyaline. Conid iophores arising f rom submerged
hyphae, erect, slightly rough walled , with walls th in and yellow-coloured , septate,
spread ing abundantly all ove r the colony and up to 480 .tm in lengt h by 2-2.8 .tm in
diamete r.
Penicilli borne singly on the conidiophore or on strongly divar icate branches.
Branches 8-20 by 2-3 .tm . Metulae truncate, incurved, in c1usters of 2-6 elements,
atta ining 7 .5-12 .tm by 2.5-3.0 .tm .
Some st rains do not show branches and the verticils of metulae may appa rently
be looking as monoverticillate structu res. Phialides 5-8 per metula , aproximately of
equal length , appearing fusiform tapering to a narrowed neck, 5.5-10 .tm by 2.5
.tm.
Conidia form ing short or long chains, not exceed ing, however, 200 .tm in length;
they are elliptical to subglobose, yellow coloured , ech inulate, thin walled , 3.6 .tm by
4.5-5.0
.tm .
122 . Penicillium kabunicum Baghdadi : fig. 122, colour pIat e 122 , and
SE M plate 21, (top : right).
10
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jirn
OO
OO O
110
6'
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'----J
100pm
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. . -~.'' ~;\l{ ~_. :1
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.Ieep brown s hades in nge : pcni cilli (Iig . 122, A) typically div nri cat c , co ns isrin g'
01' ve rti cil s 01' m etul ac 01' tairly eq ual lc n g th in th c vcrticil 01' b ranch cs al
rli llcrcn t nodcs al' th c co nidiophorc, bearin g cro wde d clus ters 01' phi alid cs;
ru uid iophores arising frorn th e substratum and rom aerial hyphae , smoo th
wullcd 01' partially deli cately roughen ed, scpta te , ranging frorn 300 to 500 11m
lu lcn gth by 2.0-3 .0 11m in diameter when borne directly from the substratum ,
11\( 1 not cxcecding 100 11m in length when borne a s branches from a eri al
hyphae; simple penicilli (appearing monoverticillate) a re not unfrequently
uhsc rved : metulae commonl y wedge shaped wh en arranged in verticils 01' 3- 6
r-l cmc n ts in th e wh orl , sho rt, abo ut 10-13 11m in len gth by 3 .0 -3.5 11m in
rli.u uoter a t th e base , a nd 3 .5- 5. 0 11m in di amet er at th e apex; wh en arranged
in grou ps 01' two per ve rticil: m etulae are longer a nd narrower, about 25-30
/ UII by 2.0 11m with a pices enla rge d in a vesicl e up to 4 .0 11m in di ameter;
ph ialid es bottle shaped , in cro wde d clusters 01' 2 -1 O 0 1' m or e eleme n ts in th e
vcrt icil, 11-1 4 11m by 2.8-4.0 11m, tapering to a conspicuo usly long narrowed
ncck ; conid ia globose to su bg lobose with one pointed end , sm ooth wall ed,
nbout 3.0 - 6. 0 11m by 2 .5 - 4 .2 11m in diameter (fig . 122 , B and SE M plat e 21 ,
rop: right); eon id ial ehains forming loosely div ergent eolum ns (fig . 122, C ).
Colonies upon CYA (eolour plate 122 , B a nd b) spreading broadly , att a in ing
:1 diameter 01' 60 mm in 14 days at room temperature (20 - 25C ), similar in
Ic xture to th e preeeding, but with th e dense network 01' fIoeeose white hyphae
ovcrl ayin g th e velvety bas al felt m or e eve nly, ob seuring gre a tly th e othe rwise
a bunda ntly sp oring a reas, whieh develop reddish brown shade s with th e
ripen ing al' eonidia ; eolony sur faee sulc at ed by a few shallow furrow s in a radial
pattern , a nd centre depressed ; undulating m argin surrounded by a fringe 01'
subme rge d hyphae extending about 1 mm beyond th e ae r ial gro wth ; exuda te
ubundantly produeed as amber-eoloured drops ; odo u r none; a m ber-eolou red
pigm ent diffusing int o th e su rrou nd ing agar ; eolony reverse (eolour plat e 122,
h) in reddish sienna brown shades, a nd strongly pulling th e agar away fro m th e
I'etri di sh an d cracking it ; penieilli essentially as deseribed aboye .
C olonies upon MEA (eolour plate 122, C a nd e) spread in g broadly ,
atla ini ng a di amet er a l' 70 mm in 14 da ys at room temperature (20 -2 5C),
cons isting 01'a loos er textured basal feIt , appearing deeply velvet y a nd ove rla id
by a thin, closely woven network 01' fIoeeose aerial h yphae infIu en ein g greatly
t he eolony appearanee; ve ry th in , eve n margin surrounded by a frin ge a l'
submerge d h yphae extend ing a bou t 5 mm beyond th e aerial gro wth ; heavily
sporing throughout in pal e ye llow-gre en a nd gray gree n shades (Sgu y , PI.
XXVI , 380, a nd PI. XXVII , 404 , resp eeti vely) depending on th e degr ee 01'
ripe ness 01' eon id ia; exuda te a nd od our laeking; no eolou re d pi gm ent diffusing
int o th e su rrou nd ing ag ar; eolony revers e (eolour plat e 122, e) dev eloping
yellow-gree n to brownish shades .
The speeies deseription is ee nte re d on th e typ e stra in C BS 409 .69 = VKM
123. Penicillium moldavicum MiIko el BeIjak ova : fig. 123, coIour plate
oo
~O O
r!?
00
~O~ g
B
yei '1
~
100
jrrn
l?
nicillium mo/dovicum Milko et Beljakova - Nov. Syst . Niz. Rast . Acad. Sci.
U.S .S.R. Inst. Bot. Nom. Kom arovii. 22: 255256. 1967.
M ilko and Beljakova's original diagnosis follows :
' Colonies on Czapek's agar growing rapidly, consisting of a close -textured basal
1011, more or less funiculose 2-3 mm deep, at first straw yellow coloured , becoming
it or 2-3 months pale lead gray to drab in colour, with the velvety felt shading to
polo pink orange tints, poorly sporing , brittle; colony reverse developing cinnamon ,
uru nge, to brown shades . Conidiophores not ramified or w ith 2-4 scattered
brunches (rnetulae 7), smooth walled, about 15-50 Jlm (up to 100 Jlm ) by 2-3 Jlm ,
w ith apices slightly enlarged , arising from aerial hyphae or f rom ropes of hyphae.
Tho bran ches (rnetulae) are cylindrical or club shaped, mostly 10-14 Jlm by 3-4 Jlm .
Phialides bottle shaped , about 6-10 Jlm by 2.5-3 .0 Jlm, in clusters of 3, 6 to 9 , or
ingle. Conidia are elliptical or ovoid , 3-4.5 Jlm by 2.5-3.5 Jlm, echinulate, showing
pule olive shades, arranged in short chains. Conidial structures are scattered over
11 10 colony surface . Old cultures showing sometimes brown crusts , consisting of
hrow n thick-walled cells .'
(Translated from the Russian by Prof. L. Vilas).
Ou r notes follow :
C olo n ies u po n CA (colour plate 123, A a nd a ) sp rea d in g broadly , a tta in in g a
rIi.une ter o f 65 mm in 14 d a ys a t room temperature (20- 25 C) , consisting of a
..losc-tex tu red b a sal felt, d eeply ve lve ty, overla id b y a d eeply floccos e network
11 1' h yph a e and ro pes of h yphae in flu encing greatly the co lo ny su r face, whi ch
rrm ains persist ently white , n ot furrowed, and slightly umbonate in the ce n t re;
" l'I'Y thin , eve n m arg in surrounded b y a fringe o f submerged hyphae ex te nd ing
about 5 mm be yond th e ae ria l gro w th; poorly sp oring throu gh out , not
in llu enc ing th e colo n y a ppearance ; ex udate a nd od ou r la cking ; no co lou re d
pi ~m ent d iffus in g into th e surrou n d ing a ga r; co lony reverse (co lour pl ate 123,
a) in ve ry pa le cre a m shade s; penicilli (fig. 123, A) typica lly divaricate ,
ron sisti ng of sm all clusters of m etula e of 2-3 ele me n ts in th e ve rtic il, about
7 - 13 {tm b y 3.0 - 4. 0 {tm a t the tip o f short co n id iop ho res with w a lls sm oo th,
lcw scattere d branches a t different nodes of the co n id iop ho re s, be aring
phial id es in small clu st ers o f 2- 6 eleme n ts in the ve rt icil, so me times single,
Iio rne a t different leveis from th e m etul a or th e co n id iop hores; co n id iop hores
vc ry short, n o t exceed ing 50 tut: in len gth b y 2 - 3 {tm in d ia me te r; phia lid es
ho ttle sh aped , a bou t 10-1 3 p.u: b y 3. 0-3.5 tutv, sometimes lo nge r , up to 15
I~ m , tapered to a cons p icuo usly long , narrowed n eck ; numerous co iled subm crged hyp ha e , su gge stive of as comata initia ls or nematode tr a ps , have been
obse rved by u s , not reported in the or ig inal diagnosis (fig . 123, O ) ; conidia
a l first st rongly ellip tical, w ith co ns p icuous co n nective s ad he r ing a t the tip s,
lending them a lim oni form p attern , becomin g la ter m ore or less globose,
124. Penicillium onobense Ramrez el M artnez: fig . 124, colour plate 124
and SEM plate 21 , (centre: right).
Penicillium onobense Ramrez et Martnez - Mycopathologia. 74. 35-49, 1981.
:,:<
11J)@@@)
A~ '
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- _'o ).;-
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100 JJm
lc.
mostly 10-1 :3 j,tm by :3.0 - 5 .0 j,tm wit h a pices ITlOI'C 0 1' lcss vesiculoso: phi alides
div er gent in crowdc d clus ters 01' :3- 6 clemc n ts pcr vcrti cil , conspicuo usly
tapering to a definite short narrowed ncck , bottl c sha pcd , common ly
measuring 8 .0-1 2 j,tm by 2.8- 3.5 j,tm ; conid ia stron gly elliptical to subg lobosc ,
with wall s roughen ed, with roughness a rra nged in spiral bands as Penicillium
brasilianum Bati sta ; con id ial chains a t first loosely diver gcnt , bccoming tanglcd
with ag e (fig. 124, C ).
C olonies up on CYA (colour plat e 124 , B and b) spreading broadl y , a tta in ing
a diamet er 01' 65 mm in 14 days a t room temperature (20 - 25C) , finc1y
velve ty, simila r in texture to the preceding bu t more irr egul arl y wrinkled by
numerous furrows; thin eve n margin with sub me rged hypha e extend ing about
2 mm beyond the ae r ial growth; spor ing ab unda ntIy in marginal zones in
brownish shades (Sguy , PI. XLVII , 694) ; non- sporulating areas in palc
pinkish sha des; exudate limited in a mo u nt, colourless; odour " mo uldy"; no
colou red pigment diffusing into the su rround ing agar; colony reve rse (colou r
plat e 124 , b) devel oping yellow ochre to pale ora nge sha de s; penicilli
essentially as described abo ye .
Co lon ies up on M EA (co lour plat e 124 , C a nd c) at ta ining a di ameter 01' 60
mm in 14 days at room temperature (20-2 5C) , velvety, plane, surfaec
appearing gra nular owing to the sea tte red tufts 01' white floceose ae r ial hyphac
a rising from th e basal felt; ve ry irregul arly dissected margin surrounded by a
frin ge 01' stol on -like subme rge d orange hyphae ex tend ing 10 01' mor e mm
b eyond th e ae rial gro wth; heavil y sporing throu ghout in blue-gray shades
(Sg uy, PI. XL , 598) : exudate and odour laeking; conid ial structures ar ising
eithe r from a netw ork 01' aerial hyphae 01' from the stolon -like submerged
hyphae, otherw ise, essentially as described aboye .
Penicillium onobense description is cente red up on th e typ e strain IJ FM 3026 =
AT C C 42,225 an d several othe r strains, isolat ed fro m a n a ndosol under beech
forest , in th e provi nce 01' N av a rr a , Spain, by DI' A .T. M a rt nez, April , 1974.
Penicillium onobense is quite a distineti ve spee ies character ized by its typ ically
di varicate pen icilli , by its bottIe-sh aped phi alides with cons picuo us tap ering
sho rt a nd narrow necks, by the production 01' ora nge stolon- like subme rge d
hyphae upon MEA , by its spirally rou ghen ed elliptical con idia , and by its
broadl y spread ing colon ies up on all substrata . The div aricat e charac ter 01' its
fru iting structu res warrant placem en t near the Asy m me tr ica - d iva ricata
altho ug h it ca nnot sa tisfactorily be ass igne d to any 01' R ap er and T ho m' s
(1949) well-recognized series, hen ce its inclu sion here .
t;;"lo
S:t:J ~ ; ,:
B ""
~;rc~~@
,
,
i
100~m
lI~(r
~.
2.0- :U) /lm, with apices e nla rge d in a vesicle up lo 4 .5 /lrn in di arn ct er:
ph ialides div ergenl , in clu ster 01' :3- 10 c1ern ents in the vcrticil , enlargcd al thc
b a se th en consp icuo us ly tapering abruptly to a sho r t na rrow ed neck, mo stly
7- 9 /lm by 2 .5 -4 .0 iuu ; co n id ia globo se, cons pie uo us ly sp inulose (fig . 125, B,
a nd SEM plate 2 1, b ottorn : left ), co m rno nly 3. 0 -3.5 /lm in di amet er; eon id ia l
chains typicall y adhe r ing in loose di vergent well -defined colu m ns (fig . 125 , C ).
Colon ies upon CYA (co lo ur plat e 125, B and b) a ttain ing a di ameter 01' 50
m m in 14 d ays a t room temper ature (20-25 C), co ns isting 01' a simila rly
tex tured b a sal felt as described a boye , but with d eep er radial furrow s;
remaining persistently white ow ing to th e deep close ly wove n net work 01' whi tc
ae rial hypha e overl aying th e basal felt; poorly spo r in g ; m argin e nti re; exu date
as pal e ye llow drops; odo ur lack in g; a m be r-co lou re d pigment diffusing into th e
su rrou nd ing a gar ; colony reverse (co lou r plate 125 , b) developing pale ye llow
to cream shade s; penicilli esse ntially as described a boye .
Colo n ies upon M EA (co lour plat e 125 , C and e), sp read ing b ro adly ,
a ttain ing a di ameter 01' 50 mm in 14 d ays a t room temperature (20 -25 C ),
co ns isting 01' a looser textured basal felt , ve lve ty to loosely lanate in m arginal
a reas which rem a in persistently wh ite; su b margin al a nd ce n tral a reas typi cally
ve lve ty, zo na te, n ot fu r ro we d, plane , h eavily sporing through out in deep bluegreen shades (Sguy, PI. XXX , 447 ); ex u date a n d odo ur lacking ; reve rsc
co lou rless w ith a gr een cast sho w ing throu gh (co lou r pl at e 125 , c); n o colou red
pi gment diffusing into the surrou n d ing agar; penicilli esse nt ia lly as described
aboye b ut with con id ia l ch ai ns form in g m ore compact di vergent colu m ns .
Species description is ce n tere d upon the typ e strain IJFM 5 144 = AT C C
42, 229 , isolated fro m th e a ir 01' M adrid , Spain, in May, 1977, by th e au th or
(C . R .). It is at present known on ly fro m th e typ e strain .
Penicilliu m castellonense is a distin ctive species charactc r izcd by its typi cally
di va rica te pen icilli , sho r t-poin te d phialides, b ro adly sp read ing colon ies upon
m ost su bstrata, spin u lose glob ose co n id ia, a nd by its both floccose a nd velve ty
characte r 01' th e colon ies upon MEA .
It a ppears to be m ost sa tis fac to r ily ass ignab le to the Asymmetricadi varica ta su b-sec tion , but it ca n no t be placed in a ny 01' Raper a nd T ho m ' s
( 1949) recogn ized series, hence its in clu sion in th e P. brasilianum se ries .
/l m b y
hclicvcd to he closcly rc1at ed to spec ics included in thc ASYlIllIlc lric a .Iivnricat a sub -sec tion. H ow evc r, it is pr escnt cd hcre as a spec ics of dubi ous
posit ion and ha s not been inc1uded in th e keys lo spcc ies sincc the au tho r of th e
prcsc nt study was not able to exam ine anc1 st udy th e type strain of th e spec ics
in qu estion because a11 a tte rn p ts to obtain it from Internati on al C u lture
(lollcctions failed .
Chalabuda's original description follows:
'Colonies upon Czapek's agar tanate . growing rather restrictedly, developing
live-gray tints; exudate as crystal c1ear yellow drops; colony reverse developing
orange colours.
Colonies upon sugar beet root extract agar lanate, more or less circular, at first
creamy white, becoming ashen-olivaceous; exudate as numerous minute crystal
clear uncoloured droplets; colony reverse orange -brown; conidiophores 90-100 JLm
by 2 JLm , with walls commonly delicately roughened, w ith terminal divaricate
branches; phialides 7 JLm by 1.6-2 JLm ; conidia ellipsiod, smooth walled, 2.5-3.0 JLm
by 1.8-2.0 JLm in diameter, appearing yellow olivaceous in mass. The type strain
was isolated from soil in the environs of Kiev.'
(Translated from the Russian by Prof. Or. L. Vilas).
Penicillium impar Von Szilvinyi - Zentbl. fr Bakt. Parasitenk . Infek . Hyg. Abt. 11.
103: 164. 1940-41. See also M.M . Kulik, 'A Compilation of Oescriptions of New
Penicifium Species'. Agric. Handbook No . 351. Agric. Res. Ser. U.S. Oep.
Agric. p. 44. 1968.
divaricata; rnetula e 5 by 2 .tm. Phialidas 5 by 2'Lm. Conidia smooth, globosa, 2.5 .tm
in diametor, producinq chains loosely divergent . Minimum temperature of growth
goC, optimum temperature 26C, and the maximum temperature is 26C to 36C.'
(Translated from the German by the author).
Penicillium vitale Pidoplichko et Bilai - In. V .1. Bilai's 'Antibiotic-Producing Microscopic Fungi', Kiev, p. 44. 1961. See also M.M. Kulik, 'A Compilation of
Descriptions of New Penicillium Species'. Agric . Handbook No . 351. Agric. Res.
Ser. U.S . Dep. Agric. p. 79. 1968.
Penicillium vitale Pidoplichko et Bilai is a sp ecie s described in 1961 whi ch is
believ ed to be closel y rel ated to th e species bel on ging to th e Asymmetricadi varicata sub-sect ion . However , it is presented here as a sp ecies of dubiou s
p osition a nd has not b een included in th e keys to species of th e cu r re n t study
for rea sons id entical to th ose for th e two p receding species .
Pidoplichko and Bilai's original diagnosis follows:
'Colonies upon malt extract agar at first white, becoming then blue-green ,
velvety, c1ose-textured and furrowed, with a rather broad white margin, developing
greenish dirty gray shades in age. Colony reverse at first straw yellow, turning to
yellowish cinnamon in the centre and yellow in the margin, shading to cinnamon in
age. With agar turning green in colour with the growing culture. Exudate as
numerous yellow drops on the surface of young colonies.
Vegetative hyphae white. Coniophores are smooth walled, 2-3.5 .tm in diameter,
with penicilli in c1usters, with phialides forming whorls at the tips of the
conidiophores (appearing monoverticillate) or more commonly in verticils of 2-3
metulae at the end of the conidiophores. Phialides are bottle shaped, 6-g .tm by
2.0-2.5 .tm, usually in more or less compact c1usters of 2-5 elements in the verticil.
Metulae measuring g-10 .tm by 2-3 .tm. Sometimes penicilli arising from a single
divaricate branch borne at a different level on the conidiophore, being then
frequently longer. Conidia elliptical or more often ovoid when young, 2.5-3 .tm by
2.0-2.5 .tm; becoming more or less globose, broadly elliptical, round-ovoid ,
2.5-4.4 .tm by 2.6-3.0 Lm , smooth, green in mass, forming loosely parallel to
tangled chains.
The maximum temperature growth on malt agar is 37-3goC .
Colonies on Czapek's agar plus 15% gelatin and glucose growing more slowly,
greenish blue, with green shades predominating, velvety, with rather wide white
margin, usually with abundant exudate as golden yellow drops in conidial areas,
turning later on dirty gray in colour; colony reverse in olive cinnamon shades,
becoming darker in age. The gelatin is similarly coloured in olive shades and
Iiquefies with difficulty. The gelatin starts to Iiquefy during the second week of
growth (at 23-25Cl. The Iiquefied gelatin has an alkaline reaction.
."".1
olonies upon Czapek's agar plus suerosa are at first white , then blue-green,
velvotv, with wide white margin, turning dirty gray in age. Colony reverse at first
traw yellow, turning to yellow-cinnamon shades. Green-coloured pigment
:lIHusing int o the surrounding agar. Exudate abundantly produced as yellowish
rops in young colonies .'
(Translated from the Russian by Prof. L. Vilas).
haptcr 5
Asyrnmetrica-velutina sub-section
pcni cilli developing, ch a rac te r istically , into a dense and eve n st and so as to
produce a velvet y appearance , in wh ich th e vegetative mycelium is largely or
torally sub m er ged in th e substratum , a n d the aerial portion of the colon ies
run sists of erect conidiophores arising directly from the substratum like a field
01' cereal. Actually, th e term velvety is broadened enou gh here so as to include
1he m any gradations that m ay be enc ou n te red, encompassing those species
closely related but with conidiophores arising from a basal network of interwoven aerial hyphae. Flocco se , lanate, funi culose , a nd fasciculate or
xynnem a tou s forms are exclu ded from this sub-secti on .
Six series were recognized by Raper and Thom (1949) in th e Velutina and
maintained in the present st u dy as follows : Penicillium citrinum se r ies, ch a r acicri zed by as ymmetric penicilli con sistin g usually of terminal verticils of
metulae , bearing phialides producing con id ia l chains typically ad he r in g into
d ivergent columns . Penicillium chrysogenum series , ch a racte r ized by penicilli that
are often ramified several times below th e level of the m etulae , with con id ial
cha ins com m on ly adhering into well -defined colu m n s, a n d by colo n ies typica lly producing bright ye llo w pi gments in ex udate a nd colony rev erse. It is
represented actually by the sin gle species of th e same name , because th e
members of th e series recognized b y Raper a n d T ho m (1949) , namely : P.
meleagrinum Biourge , P. notatum Westling , and P. cyaneo-fuloum Biourge, h ave
been reduced to synonymies with P. chrysogenum Thom (Samson et al. 1977) .
The species is well kn own for the production of th e a n tib iotic penicillin .
Penicillium oxalicum series , ch a r acter ized by the str on gly elliptic a l conidia which
in new isolates ofte n form heavy crusts , which ch aracteristically fall away en
masse if th e cu ltu re dish is tapped . Penicillium digitatum series , actuall y
represented b y two species : P. digitatum Sa ccardo a n d P. [aponicum Smith . The
former sp ecies b eing responsible for th e so-called 'green rot' of citrus fruits .
Penicillium roquejorti series , including th e moulds u sed for th e production of
Roquefort-type cheeses, ch a r ac ter ized by colo n ies with a rach noid m argin and
by con id iop h ore s with walls conspicu ously roughened . Penicillium breuicompactum ser ies, ch a r ac te r ized b y its unusu all y co m pact p enicilli in which a ll
ele men ts a re closel y compacted and appres sed , b y th e rather restricted gro wth
01' th e colon ies up on certain suhs tra ta , ami by th e develop mcnt 01' stolon -lik..
submerged hyphac along th e colony m argin 01' most forms up on ccrta in substrata .
," 1' /
,r n l
,l " ':}
C olonics grow ing rathcr restrict cdl y upon C za pek's agar, thin bu t va ry ing
in dcpth anel tcx tu rc dep cn ding upo n th e spcc ies and strain, with conid ial areas
uppca ring velve ty , m or e or less heavily sporing , developing blue-green ,
yellow-green to olive gree n shades, often prod u cing exud ate more or less
ihu nd antl y , ra nging fro m co lou rless to brigh t yellow; colony reverse u suall y in
ycllow , ora ng e to redd ish b ro wn shades, in one species becom ing almost black.
C onid iopho res erec t , com paratively short, u su all y not exce eding 200 Jlm in
k llgth, ar ising d irectly fro m th e su bs tratu m or the basal felt in a dense sta nd,
lcss requen tly fro m aerial hyphae in more floccose strains, sm ooth walled,
usu ally not ra m ified, excep t a t th e ti p o
Pcnicilli typ ically cons isting of a n ap ica l cluster of 3 - 5, or occas ionally more
di vcrging m ctula e , eac h su p porti n g a crowde d cluster of p hialides taper ing to a
xho rt narrowed neck . Conid ial cha ins com monly adhering in to we ll-d efined
:l I I div erge nt colu m ns.
Conid ia com para tively small , not exceed ing 3.5 Jlm in d iameter, globose to
r-lliptical de pendi n g upon the species and st ra in, with wall s smooth or nea rly
so .
K cy to species
Page
1. Penicilli seldom ramified below the rever of metulae , with metulae often
more or less divergent.
P. citrinum series . . .. 349
A. Metulae typically in verticils of 2-3 elements in the verticil, often of
unequal length; conidial chains not forming well-defined columns;
conidia elliptical to subglobose; colony reverse developing almost
green-black shades in certain areas.
P. corylophilum Dierckx .... 353
B. Metulae typically in verticils of 3-5 elements in the verticil , bearing
phialides in crowded elusters and eonidial chains usually in welldefined columns; conidia globose to subglobose.
1. Conidial areas in bright blue-green shades; colony reverse developing bright yellow to orange-pink shades; usually producing eitrinin.
P. citrinum Thom . . . . 351
2. Conidial areas developing dull blue-green or yellow-green shades;
colony reverse showing dull yellow to buff shades; not producing
citrinin.
P. steckii Zaleski . .. . 355
CO IOlll'
000
B
000
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00
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"
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Penicillium citrinum Thom - Bull. Bur. Anim. Ind. U.S . Dep. Agric. 118: 61-63.
1910. Emmended by Thom in 'The Penicillia', pp. 256-257. 1930; also Raper and
Thom , 'A Manual of the Penicillia ' , pp. 345-350. 1949.
Synonym
Penicillium aurifluum Biourge - Monographie, La Cellule. 33: 250-252. 1923.
cvidc nt in so rne strni ns , not in o thcrs; conid ia l a rcas a l firs: in pal c blu c-grcen
shaclcs (Sguy ; PI. XXVII , 4(0), bccoming a rtc m isia grce n al maturi ty
(Sguy, PI. XXVll , 41B), bccorning 1lI 0U SC gray lo dce p olivo gray (S guy , PI.
XXIX , 428) in age; ex udate a bu nda n t as pale yellow lo stra w-colo u red
d ropl et s; odou r strong, ' mouldy' in sorne stra ins, faint in othe rs: ycllow to
ora nge -colou re d pigmcnt di ffu sin g in to the su rrou nd ing ag a r; eolo ny reverso
(colour pIa te 126, a) in ora nge shades 01'ten assu m ing a pinkish tin t; penicilli
typi eall y eo ns isting 01' a terminal who rl 01' 3, 4 , or occasionally m ore , some what
di vergent m etulae , mostly 15-28 p.m b y 2.5- 3 .0 p.m , with a pices enlarged in a
ves icle up to 4 p.m in di ame ter (fig . 126, A) ; eon idiopho res smooth walled ,
a rising m ostIy from th e su bstratu m or from a erial hyphae in th e colony ce n tre
or in /loeeose strains, usu all y 50-200 p.m in length by 2.5-3 p.m in di ameter;
phi alides more or less erowde d a nd pa rallel , in clu sters 01' 6- 10 or more
elemen ts in th e ve r ticil, a bo u t 13-1 7 p.m b y 3-3 .5 p.m in sorne st ra in s, or 8 - 11
p.m by 2-2 .8 p.m in others, usu all y tape ring to a co ns picuo us sho r t n arrow ed
neck; eon id ia globose to su bg lobose, mostly 3-3 .8 p.m , smooth -wa lled or
nearl y so (fig . 126, B a nd SEM plate 21, bottom : ri gh t) .
Colonies upon C YA (co lou r plat e 126, B and b) gro wing more rapidly th an
aboye, a tta in ing a dia meter 01' abou t 35 m m in 14 d ays at room temperature
(20 - 25 C ), eo nsis ting 01' a tough, close-textured basal felt, typieall y velvety,
eons pieuously zo nate, strong ly radi at ely wrinkled an d raised in th e co lony
cen tre in a erate riform pa ttern ; white eve n m argin surro u nded b y a narrow
fringe 01' su b merged hyphac exte nd ing abo ut 1 mm beyon g th e ae ria l grow th;
heavily spo ring in zo nes , vario us ly coloured in blue-green (Sguy, PI. XXX,
442), blu e-gray (Sguy, PI. XXXII I, 493) or brow ni sh shades, d ep ending
up on th e d egree 01' m aturity 01' eonidial areas ; ex udate a b u ndan tly produced ,
as light yellow d ro ps; odou r fain t , aro matic; ambe r-coloured pigment di ffusi ng
into th e su rro u nd ing agar; eolo ny reverse (co lo u r plate 126, b) in deep ora ngered shades; penieilli esse ntially as deserib ed aboye .
C olon ies upon MEA (co lour pla te 126, C and e) gro wing m ore restrictedly
th an up on CA , a ttain ing a d iamet er 01' less th an 20 mm in 14 d ays a t room
temper a ture , not furrow ed , velvety, with cen tre sligh tly urnbonat e; with
undulati ng, thin m argin surrou nde d by a narrow fringe 01' submerged hyphae
ex te nd ing abou t 1 mm beyond th e aeri al grow th ; heavily sporing th rou gh out in
deep blu e-green shades (Sguy , PI. XXX, 446) ; ex udate laeking; odo ur faint ,
aromatic; golde n yellow pigment diffu sin g in to th e su r ro u nd ing agar ; col on y
reverse (eolour plate 126, e) in bright ora nge-yellow shade s; penicilli esse n tially
as described above ; co nid ia l chains in eom paet colu m ns (fig . 126, C ).
T he spec ies description is cen te re d upon th e typ e stra in CBS 139.45 = C M I
92, 196 = NRR L 1,841 = IJFM 3755, isolated by Ch. T ho m in 1910, and many
othe r strains isolated by th e author from Sp anish soils .
R ap er and Thom' s eonce p t 01' Penicillium citrinum is broad in seope and
includcs lorrn s whi ch va ry su bstantially in pa rt icu lar cha rac teristics, yet
posscss ccrta in basi c featurcs in cornrno n ( 1949) . In thcir opin ion , it seemcd
Hall: l o bclievc th at 75 % 01' thc strains tha t produce sorne citrinin and show
pcni cilli 01' the P. trinum-t y pc com ply full y with the spec ies description .
I 'D. Penicillium corylophilum Dierckx : fig . 127, colour plat e 127, and
SE M pl ate 22, (top: left) .
i.w.J.lJ.wJ
00
10 pm
00 0
0 8 0 0
00
i~l~
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..
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'.
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100 prn
..
Penicil/ium corylophilum Dierckx - Ann . Soc oSci. Bruxelles. 25: 86. 1901 . See also
Biourge's Monographie, La Cellule. 33: 266-267. 1923; also Ch. Thom , 'The
Penicillia', pp . 254-255. 1930; also Raper and Thom, 'A Manual of the Penicillia' ,
pp. 341-345. 1949.
Synanymies
Penicil/ium cloro-Ieucon Biourge - Monographie , La Cellule. 33: 270-271 . 1923.
Penicil/ium coeruleo-viride Smith - Trans. Brit. Mycol. Soco 48: 274. 1965. See
also M. M. Kulik, ' A Compilation of Descriptions of New Penicil/ium Species'.
Ag ric. Handbook No. 351. Ag ric. Res. Ser. U.S. Dep. Agric . pp . 29-30. 1968.
. J . J. J
murgin surroundcd by a wide fringc 01' submcrged hyphae extend ing about 5
more bcyond thc ac r ial growth; hcav ily sp or in g thro ug ho u t in deep
blu c-grcen shades (Sgu y , PI. X X X , 447); exudate and odour lackin g ; no
colourcd pigment diffu sin g int o th e surrou nding agar; colony reverse (colour
pla tc 127, r ) parti ally colou rless with locali zed zo nes, pa rticu la rly in the centre
dcvclopi ng very dark green, almos t black colou rs . Penicilli esse ntially as
dcscribed above.
T he spec ies descript ion is centered on th e ty pe strain ATCC 9, 784 = C BS
:112.48 = CM I 39, 754 = N R R L 802 = QM 7,5 10, isolat ed by L. P . D ierckx in
1901 , on strain CBS 281.36 = N RRL 779 = IJ F M 3754, isolat ed by A . von
Slviny i (as Penicillium sumatrense v . Szilvinyi) fro m tropical continenta l wa ters
i l\ 1936, and m any other strains isolated fro m soi1 in the Province 01' Navarra,
Spain , by DI'. A. T . M a rtnez .
111111 0 1'
128 . Penicillium steckii Zaleski: fig. 128, colour plat e 128, and SE M plate
O O
O
80
00
00 O
O O
O O000 0O'
8
00
O00O00
00
10 pm
~~
L.-........J
jrrn
100
eR .
Fig. 128. Camera lucida
drawings of Penicillium
steckii Zaleski, CSS 260.55.
A, Detailed draw ings of the
penicilli . S, Mature conid ia.
C, Habit sketches.
.'''\1
Penicillium steckii Zalaski - Bul!. Acad. Polonaisa Sci. Math. Nat. Sr. B, pp.
469-471.1927. See also Ch. Thom, 'The Penicillia', pp. 255-256.1930; also Rapar
and Thom, ' A Manual of the Penicillia', pp. 350-352. 1949.
Colon ies upon CA (eolou r pla te 128 , A and a) growing rather restri etedl y,
attain ing a d iameter of about 30 m m in 14 days a t roo m tem perature
(20- 25C), eons isting of a tough , close- tex tured basal felt , appearing velvety,
za nate, ra ised a nd radiately furrowed , with white eve n marg in su rro u nded by
a narro w frin ge of su bmerged hyp hae extend ing abo u t 1 mm beyond th e aerial
growth ; medium to heavy sporing in zones, depend ing of the degree of ri peness
of eonidial zo nes; conidial areas in blue-green shades (Sguy, Pi. X XX , 443)
with th e ripening of eon idia; ex udate lim ite d to abu ndant depend ing upon the
strain, as pale yellow -eoloured droplets; odour at first laeking or fain t, with age
beeoming somewhat mo uldy; very pa le amber-eo loured pigme nt d iffus ing in to
the surrou nding agar; eolony reverse (eolour plat e 128 , a) in pink ish orange
shades; penieilli typieally bive rtieillate asymmetrieal (fig. 128, A), with
eonid iophores arising from the substratum or th e basal felt, smooth wa lled ,
variable in length , but eommonly eomparatively short, seldom exceeding 200
iuu, generally less, by 2.5-3 .5 iuu , u sually not ramifi ed ; penieilli eonsisting of
a ter m inal vertieil of 3-5 m etul ae bearing eompaet whorls of phialides
produeing well-defined d ive rgent eolumns (fig. 128 , C) of eon id ia u p to 150 J-m
in len gth ; m etulae ra ngi ng from 12 to 18 tui: by 3 - 4 J-m; phialides in erowded
clusters, parallel , about 8 - 12 J-m by 3.O- 4 .5 usn, bottle shaped, ta pe ring to a
conspieuous, short , narrowed neek; eonid ia comparatively small, globose to
subglobose, abo ut 2.0-2 .5 J-m in d iameter, with wa lls smooth or del iea tely
ro ughened (fig. 128 , and SEM plate 22, top : right) .
Colonies u pon CYA (eolour plate 128 , B and b) attaining a d ia mete r of 35
mm in 14 days at room tem pera ture (20 -25 C), similar in text ure and eolony
appearanee to the preeedi ng, but more deeply radiately furrowed a nd less
ra ised; heavily spori ng in zo nes in bl ue -green (Sguy , Pi. XXX , 443) to graygreen shades (Sguy, Pi. X X X V , 519), depen di ng on the degree of ripeness of
eonidia; exudate very abundan dy prod uced, as la rge yellow -eoloured drops;
odour laekin g; pale amber-eoloured pigme nt diffusi ng in to the surroundi ng
agar; colony reverse (eolour plate 128, b) in yellowish orange shades ; peni eilli
essentially as deseri bed aboye .
Colonies u pon M EA (co lour piate 128, C and e) attaini ng a diameter of 30
mm in 14 days at room temperature (20- 25 C) , eonsisting of a looser textu red
basal felt, fla t , with seetors of aerial, floeeose , vegetat ive, whi te h yph ae overlaying th e basal felt, with very thin margin surrou nded by a wide fri nge of subm er ged h yphae extendi ng abo ut 5 m m beyond the aerial growth; heavily
spo ring throughout in deep a rtemisia green shades (Sguy , Pi. XXI, 308) ;
exudate and odour laekin g ; no eoloured pigment d iffus ing into the su rrou n-
:1;17
d ill!{ agur; co lo ny reve rso (co lour plat e 1211 , e) in paj e yellow sha des, bccoming'
oru ngc-ycllow in th e ce n tre ; pcni cilli casc nt iully as dcscribed a hoye.
T he spcc ies description is ccntc red llp on stra ins CI\S 260.55 = CM140,583 =
\'['cc 10,499 = NRR L 2, 140 = Q M 6,41 :~ = I./FM 7211 , isolat ed by Prof, Wm .
11. W cston fro m a sa m ple 01' fabric ex pos cd on Barro Colorado Island ,
l'uu ama , 1948 , a nd from othe r stra ins isolatcd from so il in th e Province 01'
Nav arra by Dr. A.T. Martnez , 1975. (Colou r pl ate 236 presents a representa tiv e strain 01' P. steckii isol at ed fro m sea sa lt. )
129. Penicillium matriti* Smith: fig. 129 , colour pl at e 129 , and SEM plat e
22 , (ce ntre : left),
Lw.L!.wJJ
10 urn
o
0) 0
O O O
0
0 00 0 0 0
00 00
." o/ o;""~';f1;,?
00
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"\
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~'"l~'" ,
'",\,
Penicillium matriti Smith - Trans. Brit. Myeol. Soe. 44: 44-45. 1961. See also M.M.
Kulik, 'A Compilaton of Deseriptions of New Penicillium Species' . Agrie.
Handbook No. 351. Agre. Res. Ser. U.S. Dep. Agrie. p. 52. 1968.
N ote of the a u thor: The bi no mial P. madriti has been fou nd to be orthogra phically incorrect o
T he correc t design ati on , namely P. matriti , is ther efore prop osed.
Smith's original diagnosis follows, since this adequately presents the species in
question when first isolated:
'On Czapek agar grows fairly rapidly, pale blue-green , turning gray-green, with
white edge about 2 mm broad during the growing period, velvety but tending to
become somewhat heaped in the centre; drops numerous, small, yellowish; reverse
peach coloured, indistinctly zonate ; on malt agar grows very rapidly, grayish bluegreen, thick velvety, with numerous yellow drops and reverse very reddish brown;
penicilli with metulae each bearing a compact cluster of phialides, branching below
the metulae not observed ; conidiophores arising from the substrate, long, smooth,
2-3 m in diameter; metulae slightly swollen at the apex, 10-11 m by 2.5-3.5 m;
phialides tapering rather gradually, but not acuminate as in the Biverticillata, 7-8
m by 1.5-1.8 m; conid ia globose, finely roughened, 2-3 m, mostly 2-2.5 m in
diameter, conidial chains forming a single fairly compact columnar rnass, often bent
or slightly twisted.
This species appears to form a transition between the P. citrinum and the P.
chrysogenum series. In the lack of any branching below the metulae it is nearest to
P. steckii Zaleski, but the latter shows slower growth, thinner and more bluish
colonies, and spore chains in several distinct columns, one to each metula. P.
matritiwas also grown in parallel cultures along with P. cyaneo-fu/vum Dierckx, the
member of the P. chrysogenum series which it most closelv resembles . P. cyaneofu/vum has thicker, almost floccose, colonies, penicilli which frequently show
branching below the metulae, and much larger spores than P. matriti.
The type strain, maintained as Cato No. BB 389, was isolated in 1954, by G.
Agosti in this Department (London School of Hygiene and Tropical Medicine), from
garden soil from Madrid. A second strain BB 389 A, was isolated from soil from a
different garden in Madrid. On Czapek agar the latter forms slightly thicker colonies
than BB 389, and rather more brownish colour in reverse; on malt agar the two are
indistinguishable. '
phiu lide bot tle sha ped , tapcring lo a sho rt narrowcd neck , lIIostly B-IO /-UIl by
:1.0 - :i .5 JlIII; co n id ia globos e , about 2. :> - :i. O JllII in di a mctcr, a p pcr u -ing
IOlloo lh wall ed 01' ncarl y so wh cn obsc rvcd lind el' (he light microscop e ( fig . 129,
B) 0 1' dclicately rou gh en ed whe n obse rved undcr th c SE M (pIa te 22, ce ntre :
lef't ); co nid ial chains in well-defined di vcr gcnt colu mns (fig . 129, C).
C olonics upon C YA (eolour plato 129 , B a nd b) attain ing a diameter of 50
111111 in 14 da ys a t room te m pe ra tu re (20 -25 C ), eons isting of a sim ila rly
ic xtured ba sal felt bu t mo re regula rly wrinkled in a radial and conce nt r ical
pnt tcrn , with a narro w flat margin su rrou nde d by a frin ge of su bme rge d
hyph ae extend ing abou t 1 mm beyo nd th e ae rial growth; abundantIy sporing
in conspicuous zo nes in m ou se gray (Sgu y , PI. XXIX , 430) to pale pinki sh
': I'a y shades, dep ending on th e degree of rip eness of eon id ia; odour stro ng,
sllggesting am mon ia; exu date not obse rve d; arnbe r-colou re d pigment diffu sin g
i nro the sur rou nd ing agar ; penieilli esse ntially as deseribed aboye .
Colonies upon M EA (colour plate 129, C and c) gro wing rather restrietedl y ,
a llaining a di ameter of 45 mm in 14 days at room temperature , flat , not
lurrowed , eon spieuo usly zonate, whi te th in m argin surrounde d by a frin ge of
subrnerged hyphae extend ing about 2-3 mm beyond th e ae rial gro wth; eolony
surface typi call y velvety , ove rlaid in sectors by a thi n closely woven network of
white ae rial h yphae obse u ring th e ot he rw ise heavy spor in g are as; heavily
spo ring throughout in blue- gr een shades (Sguy , PI. XXXI , 462); exudate an d
od our laeking ; no eoloure d pigment diffu sing into th e surrounding agar;
ro lony reverse (colou r plate 129 , e) developing sien na reddish brown colou rs in
loealized seetors eorres po nd ing to th e heavily spo ring are as, and pale yellow
sha des in seeto rs eorres po ndi ng to th e ove rgrowth of vege ta tive floceose
hyphac ; penicilli essentially as described aboye .
The spee ies deseription is een tere d up on th e typ e strai n C BS 347.6 1 = CMI
B6,563 = ATC C 18,223 = Q M 7,959 = IJ FM 7128 , isolat ed from ga rde n soil in
M adrid , 1954 , by G. Agosti .
M embers of th e Penicillium citrinum series occu r upon a wid e va riety of substrata su bject to soil conta m ination including cott on a nd othe r fabrics, dairy
and food product s, tobacco, leather goo ds, and vegetable m at eri als undergoing
slow decomposition. They are com mo nly isolat ed from soil. They have been
cncou ntered more frequently th an any othe r Penicillia upon deteri or ating
m ilita ry equ ipm ent in tropical and su b- tro pical cou ntries. They m ay be
isolat ed from len s mountings in photogr aphic ca me ra s, with th e mycelia
exte nd ing ou t upon the glass surfaee and etching it sufficientIy to render it
praetically us eless .
Penicillium
dlly ,\'o,l.tt:7lUm
seri es
O U T STANDIN G FEATU R ES
Colon ies typi cally velvety, more 0 1' lcss clo se textured , with co nid iopho res
arising frorn the subs tra tu m in a dense stand ; in sorn e strain s more 01' less
floccose , with conid iopho res borne as bran ches from ae rial hyphae ; com m only
radiately furrowed .
Exudat e com mo nly abu ndantly pradu ced as bri ght yellow drops.
C olony reverse usually in th e same b right-yellow tints, becoming brown in
age, with the su rra u nd ing agar sim ila rly colou re d .
Peni cilli typi cally asy m metrical, with con id iophores smo oth walled,
irregul arly ramified in larger stru ct ures, a l' u su ally cons isting of a terminal
wh orl of m etulae in smaller pen icilli; with con id ial chains formin g well-de fined
compact colu m ns eac h arising from individual ve rticils of phialides.
Conidia sm oot h- walled a l' nearl y so, va ry ing from globose, su bglobo se to
definitely elliptical, dep ending on th e strains, and rangin g in size from abou t
2.5 to 4.5 flm in di ameter.
The series is a t present represented by a single species, Penicillium chrysogenum
Thom , since th e three othe r spec ies recognized by R ap er a nd Thom (1949 ),
namely: P. meleagrinum Biourge , P. cyaneo-fulvum Biourge , and P. notatum
W estling have recently been reduced to syno ny m ies of th e former species
(Sa mso n et al. 1977 ).
Penicillium chrysogenum Thom is espec ially commo n in natu re, occ u rrin g fairly
regul arly in soil and up on a wide variety of org an ic su bstra ta. It has received
particul ar atte n tion during th e past few decades after th e di scovery of th e a ntibiotic penicillin by P rafessor Alexander Flemin g in 1928, wh ose interest was
revived in 1940 thraugh th e demon strati on of its pot ential th erapeutic value by
Professor s E . C ha in, H.W . Florey , and others at Oxford University , UK.
The species is quite va riable in pigmentati on , exudate production , colony
texture , rat e of grawth , etc . , dep ending upon th e strain, yet all show th e
gene ra l charac teristics indicat ed aboye and ca n be readily recognized.
In th e m orphology of th e peni cilli , th e va ria tion m ay range fram stra ins in
which th e penicillu s cons ists of a conidiophore bearing a serie s of branch es ,
with eac h praducin g a m on overticillat e structu re, to a com plex a nd typi cally
asymmetrical appa ra tus. The conid ia may vary from globose, su bg lobose to
defin itely elliptical, and fro m quite small , with di ameters m ostly less th an 3 .5
tui, to strains with conid ia up to 4.5 flm in th e long ax is.
130 . Penicillium chrysogenum Thom : fig. 130 , colo ur plat e 130 , and SEM
plat e 22 , (centre : ri ght) .
Penicillium chrysogenum Thom - Bull. Bur. Anim . Ind. U.S. Dep. Agric. 118:
00 o
000
O
000
B
O 00Wul=J
10jJm
Penicillium citreo-roseum Dierckx - Ann. Soc o Sci. Bruxelles. 25: 86. 1901; sea
also Biourge's Monographie, La Cellule. 33: 182-184. 1923; also Ch. Thom, 'The
Penicillia' , pp. 265-266. 1930; also Raper and Thorn, 'A Manual of the Penicillia',
p . 373. 1949.
Penicillium chlorophaeum Biourge - Monographie, La Cellule. 33: 271 -273.1923.
See also Ch. Thom, 'The Penicillia', pp . 262-263.1930; also Raper and Thom, 'A
Manual of the Penicillia', pp. 363-364. 1949.
Penicillium cyaneo-fulvum Biourge - Monographie, La Cellule. 33: 174-176. 1923;
See also Ch. Thom, 'The Penicillia', p. 267. 1930; also Raper and Thom, 'A
Manual of the Penicillia', pp. 371-372. 1949.
Penicillium fluorescens Laxa nomen nudum (?) - It was cited but not described by
O. Laxa, Zentbl. Bakt. Parasitenk. Inf. Hyg. 11 Abt. 86: 164-165. 1932. See also
Raper and Thom , 'A Manual of the Peniciltia', p. 364. 1949.
Penicillium griseo-roseum Dierckx - Ann. Soco Sci. Bruxelles. 25: 89. 1901. See
Biourge's Monographie, La Cellule. 33: 168-170. 1923; also Ch. Thom, 'The
Penicillia', pp . 263-264. 1930.
Penicillium meleagrinum Biourge - Monographie, La Cellule. 33: 147-149. 1923.
See also Ch. Thom, 'The Penicillia', pp. 266-267. 1930; also Raper and Thom, 'A
Manual of the Penicillia', pp. 364-367. 1949.
Penicillium notatum Westling - Arkiv. Bot. 11: 55, 95-97. 1911. See also
Biourge's Monographie, La Cellule. 33: 179-181. 1923. Also Ch. Thom, 'The
Penicillia', pp . 264-265. 1930; also Raper and Thom, 'A Manual of the Penicillia' ,
pp. 367-371. 1949.
Penicillium roseo-citreum Biourge - Monographie, La Cellule. 33: 184-186. 1923.
See also Ch. Thom, 'The Penicillia', p. 323. 1930; also Raper and Thom, 'A
Manual of the Penicillia', p. 364. 1949.
Penicillium rubens Biourge - Monographie, La Cellule. 33: 265. 1923. See also
Ch. Thom, 'The Penicillia', pp. 249-250. 1930; also Raper and Thom, 'A Manual
of the Penicillia', p. 364. 1949.
th rou ghour in most stru ins, in o thcrs o tcn sho wing sorne tcndcn cy lo rc mn in
srerilc in centra l arcas with veget ati vo hyph ae ycllowish to crc a m colou rcd,
Iu:collling dcc p bl ue grcc n (S guy , PI. X X X , 447 ) with the ripc ning 01' con id ia;
cxuda te ab unda nt ly produ ced in most stra ins, as golde n yellow co ns picuo us
dro ps ; odou r lacking 0 1' ind efinitc ; bright yellow-coloure d pigme nt diffusin g
l l lo (he surro und ing agar; colony reverso (colour pla te 130 , a) in bri ght yellow IIl'ange shades; conid iophorcs arisin g mostly fro m th e su bstratum in a den se
xtund , smoo th wall ed , variable in leng th, us ually rangin g from 150 to 400 J-t m
111' mor e in length by 3.0-3 .5 J-tm in di am eter, occasionally bo rne fro m aerial
hyph ae a nd shorte r, abo ut 50- 75 J-tm in leng th, with ap ices somewhat inflat ed ;
pcni cilli asy mmetric (fig . 130 , A) , commonly branched , with branch es a nd
nurin axes each usu all y bearing 2 -4 metulae to form a fairly large peni cillu s,
k ss com mo n ly cons isting 01' a simple terminal vertic il 01' m etulae only, bea rin g
rl us ters 01' phialides producing con id ial chains up to 50 - 75 J-tm in length ,
uu igled 0 1' adhe re nt int o fai rly well-defin ed colu m ns; branches va riable,
ro m mo nly 15- 25 J-t m by 3.0 -3 .5 J-tm ; me tulae u su all y 10-1 3 J-tm by 3 .0 - 4. 0
tIJl, occasionally lon ger; phialides bottl e shaped, in fairly com pact cluste rs 0 1'
'1- 6 ele me n ts in the vertici l, m ostly 8 - 10 tut: by 3.0-4. 0 J-tm but va riable in
d ilferent strains, abru ptly ta pered to a sho rt narrowed nec k; conidia ellipt ical,
su bglobose 01' globose, dep ending u po n th e stra in, mostly 3.0 -4.0 J-tm by
L.B- 3 .5 utt , occasionally larger, smooth wall ed 0 1' nearly so wh en observed
lindel' th e light microscope (fig . 130 , B) but del ica tely ro ughe ned wh en
observed under th e SEM (piat e 22, ce ntre: right) ; coni dial chains in more 0 1'
lcss defin ed colu m ns.
Co lonies u po n C YA (colour plate 130, B and b) spread ing as upon C A,
altaining a d iame ter 0 1' abo u t 50 mm in 14 days at roo m temper ature
(20- 25C), sim ilar in tex ture to the precedin g but heavier sporing throu gh out
in mos t stra ins a nd conspicuo us ly zonate, in th e same deep blue-gr een shades
with the ri pening 0 1' coni d ia (Sguy, PI. X XX, 447); with thin margin
surrou nde d by a fringe 01' su bmerge d hyphae exte nd ing abou t 3 mm beyond
the ae rial growth; strong ly radiat ely furrowe d and pulling the agar away and
crac king th e colony su rface in th e centre as deep crevices along th e ra d ial
Iurrows; ex udate abu ndantly produced as large , b right golde n yellow drops;
odo ur lacking ; bright golde n yellow-coloured pigment di ffus ing abu ndantly
int o th e surrou ndi ng agar; colony reverse (colou r plate 130 , b) develop in g deep
ora nge -yellow shades; peni cilli esse ntia lly as descri bed a boye bu t cons istently
heavier spo ring .
Colonies upon M EA (colour piate 130 , C and c) attaining a di ameter 01'
55- 60 m m in 14 days at ro om temperatu re (20-25 C), cons isting 0 1' a looser
tex tured basal felt , flat , never fu rrowed, strictly velvety, zanat e, with thin
m argin surrounded by a fringe 0 1' su bme rge d hyphae exte nd ing abo ut 4 mm
beyond th e ae riaJ gro wth; heaviJy spo ring throu ghout in gr ay-gr een shades
(S gllY, PI. XXIX , 429); cx uda tc a nd odo u r luckin g; no COl0 11I'l: d pi gm en!
dillu sing in to th c su rro u nd ing aga r; co lo ny rev erso (co lour plat o 130, e) in dul l
yellowish, tu vinaceous or brownish drab shadc s: pcni cilli cssc ut iully as
described ab oye but tending to dc vclop better defin ed colu m ns 01' co nid ia (Ilg.
130, C).
The spec ies description is cen tered up on th e typ e stra in C BS = 306 .48 = C M I
24, 314 = AT C C 10,10 6 = Q M 7,500 = NR R L 807 = IJFM 7063, isolated by C h.
Thom , and up on com parative exa m ination al' many add itiona l stra ins
representing th e sa me basi c cu ltu ra l a nd morphologic al charac te ristics, a rno ng
th em , strain C BS 355.48 = C M ! 39 ,759 = ATCC 10,108 = NRRL 821 , isolat cd by
R. W estling frorn bran ch es of Hyssopus in Norway , 1910 , is the formerly typ c
stra in of Penicillium notatum W estling, actually syno ny mo us with P. chrysogenum
Thom . (Co lou r plat es 237 and 238 present two representati ve stra ins of P.
chrysogenum, isolat ed frorn th e a ir by th e autho r. )
lJ.J.J..WwJ
10jJm
O O
000
00 0
0 800 0
O
00
00 O
000
1~ 11
O
0
Fig . 131. Camera lucida
drawi ngs of Penicillium
chrysogenum Th om mut o
fulves cens Takashima, Ar ima
et A be ex Ramrez, CBS
319.59. A , Detailed draw ings
of the penicilli. B, Mature
conid ia. e, Habit sketc hes.
C olonies spreading broadl y , velutinou s, flat 01' irregularl y 0 1' radi at ely
furrowed; heavily sporing throu ghout in dull to deep blue- green shad es ;
velve ty ; with conid ia 01'ten formin g deep crusts whi ch break off wh en th e
culture vessel is trapped .
Co nid iophores typi cally arising from th e subs tra tu m in a den se a nd eve n
sta nd, va r iable in length but u su ally not excee ding 200 Lm ; smo oth wall ed.
Pcni cilli typi call y biv erticillat e asym me trical, cons isting 0 1' 2 0 1' more
metulae , 0 1' irregularl y branch ed , with branch es and metulae some time s
arising a t th e sa me level , 01'ten closely appres sed.
Phi alides closely parallel , com monly producing chains 0 1' conidia adhering in
loose to com pact colu m n s up to 500 Lm 0 1' m or e in len gth .
Con idia usu ally elliptical, sm ooth-walled 0 1' conspicuo usly spinulose.
K ey to spe cie s
Page
1. Penicilli typically rebranched below the level of metulae, with the main
axes and branches terminating in verticils of metulae. Penicilli commonly
long, with elements loosely arranged and often divergent. Conidio
phores smooth walled; margin not appearing arachnoid. Colonies not
producing yellow pigment in exudate or colony reverse; conidia
commonly 5 ,tm or more in long axis; elliptical or subglobose , fairly
uniform in size; common in soil.
P. oxalicum series . .. . 366
A. Conidia smooth walled.
1. Conidia elliptical; colonies plane or nearly so, with conidia often
forming deep crusts; con idial chains appearing 'silky' when viewed
under lower power.
P. oxalicum Currie et Thom . ... 367
2. Conidia subglobose; colonies radiately furrowed, with conidia not
forming crusts; colony reverse upon CYA developing deep cherry
orenge-red shades.
Th c series as recogni zed by R aper and 'I'horn has been broaden ed eno ugh as
include in it one species: Penicillium fen nelliae Stolk, add itional to th e two
wcll-marked , vclvety spec ies with elliptica l, smooth-wa lled conidia already
incluclcd in it (Rape r a nd T ho m 1949). It is a rtificial in characte r and was
introduced primarily as a m atter of conve n ience .
Alth ou gh a cla se relat ion ship is not presumed between th ese three species,
thcy do possess su fficient cha racters in com mo n to locat e th em here m or e
n -ad ily tha n any whe re else.
O ne of th e spec ies , Penicillium oxalicum Currie el Thom is world wid e in
clist ribution a nd one of th e most ubiquitou s of all th e Penicillia . It has long
I leen known to represent a normal com po ne nt of th e soil m ycoflora .
The seco nd spec ies , Penicillium atramentosum T ho m is prob abl y not close ly
rclated to P. oxalicum , and unlike it , P. atramentosum appears to be
ro rnpa ra tively rare .
lo
CJo
00
O Ooa
00
o
1i ,
L--...J
100 ).1m
at.
close-rcxturcd basal Iclt , typ ically velvcty, stro ng ly rnd iatcly urrowed and
rlcprcsscd in th e ce n tre , ov erl aid hy a thin , closely wo ven network 01' aerial
hyph ae inllucncin g somewhat th e colony appcarancc ; with a thin m argin
su rr ou nded by a fringc 01' submcrged hyph ae cx te nd in g about 3 mm beyond
h ae rial gro wth; heavil y spo ring throughou t , dev eloping gray-gr een shade s
(SI:gUY, PI. XXIX , 428 ) instead 01' the deep blue-green sho w n by th e
pr cccding ; ex udate a nd odo ur lacking ; no colou re d pi gment diffusing into the
su rr ou nd ing aga r ; colony reverse (col our plat e 132, b) devel oping reddish
h rown shades in th e ce n tre, else whe re cin namo n colou rs; p eni eil1i esse n tia l1y as
. lcscr ibe d above .
Colon ies upon M EA (co lour plat e 132 , C a n d e) sp read ing b ro adly ,
a tta ining a diamet er 01' 60 mm in 14 days a t room temperature (2 0- 25 C ) ,
l'llns isting 01' a looser textured b asal felt , very hea vily sporing forming deep
rru sts as not ed a boye, partiall y obsc u re d, es pecia lly in margin al a reas, by an
.ibundant ove rg rowt h consisting 01' a loosely woven net work 01' deeply floccos e
nc rial hyphae, influencin g greatly th e colo ny ap pearance; not furrow ed; od ou r
.m d cxu da te lacking; pale a m ber-colou re d p igment d iffu sing in to th e su rro u ndin g agar; colony rev er se (colour plate 132 , c) d ev eloping d eep purple brown
shades in th e ce n tre , elsewhere sh owing cin n a m on tints ; penicil1i essen tial1y as
dcscr ibed ab oye .
T he spec ies d escription is ce n te re d upon typi cal cu lt u res suc h a s C BS 219 .30
= NR R L 787 , isolat ed by C u rr ie a n d Thom in 191 5 , strain C BS 358 .48 = C M I
:19 ,750 = ATC C 10 ,476 = N R R L 790 = Q M 6 ,747 , isolat ed in 1934 from Blu e
Danish cheese b y S . H all , a n d upon m any othe r strains isol at ed from soil in th e
Pr ovin ce 01' N av arra by DI'. A .T. Martnez .
Penicillium oxalicum wh en long m aintained in laborat ory a rtificial cu ltu re
sho ws a m arked tendency to produce grad ual a n d progressive va r ia tion away
from th e or igin al, espeeial1y an in clination to be come more lanat e and less
hea vily spo rin g . It is cosm op olita n in its habitat and wo rld wide in distribution .
I t is espccial1y frequently isolated from soil a n d from plant residues undergoing
slow decomposition .
1I
133. Penicillium atramentosum Thom : fig. 133, colour plat e 133, and SEM
Colonies upon CA (colour pl at e 133, A and a) attain ing a diamet er 01' abo u t
45 -50 mm in 14 days at room temperature (20 -25 C) , consisti n g 01' a typi cal1y vel vety b a sal felt , co m para tive ly thin and flat, not furrowed, excep t 1'01'
-,
!! JI
e ~"""'
~l
~ ' '
L...--.....I
100pm
1t,
R.
vc rt icil, moxtl y
nnrrowed ncck ;
:111 <1 SEM pl at c
rh u in s ad he ring
C olon ice upon C YA (colour pia te 133, B a nd b) spread ing broadl y , attain ing
a diam eter (JI' 60 mm in 14 days a t roo m temperature (20 - 2S0C), cons isting 01'
a iypi cally velvety basal felt , with wid e flat m argin (ab ou t 10 mm in width)
su rrou nded by a frin ge 01' subm erged hyphae exte nd ing abo u t S mm beyond
II\(: acrial growth; su bmarg ina l and central are as deepl y and stro ngly sulc ated
Iy nu merou s radial furrow s and ove rla id by a thin overgrowth 01' fine ste rile
hyph ae influ en cin g th e colony appearance; heavily sporing th rou gh out in deep
grccn sha de s (S guy, PI. XXX , 441) particul arl y cons picuo us in m a rgin al
arcas , elsewhere partially obs cured by th e thin overgrowth 01' pinkish coloured
xtcr ile hyph ae ; odo ur lackin g ; exudate limited in amo u nt, as crys ta l-clear
.lroplets enmes he d in th e myceli al felt ; purple re d to vinaceous coloured
pigment diffusing in to th e surround ing aga r; colony reverse (colour plate 133 ,
h ) develop ing deep purple red to che rry red shades; penicilli es sent ially as
.lcscribed aboye.
Colonies upon M EA (co lour plate 133, C a nd c) at ta ining a di amet er 01' 55
111m in 14 days at room temperature (20- 2S0C), cons isting 01' a looser textured
basal felt , typi call y velve ty, azo nate, flat , not furro wed, with very thin m argin
xurrou n de d by a frin ge 01' submerged hyphae exte nd ing abo ut 5 mm 01' more
bcyond th e ae rial growth; heavily sporing throu gh out in blue-gr een shades
(Sguy, PI. XXXI , 462) wi th th e ripe ning 01' co ni d ia; colony surface showing
locali zed secto rs 01' sterile whi te hyphae slightly obsc u ring th e heavily spo ri ng
ar cas, lending grayish green tin ts to th ese sec to rs; no coloured pigm ent
d iffusin g in to th e surrou nd ing agar; colony reverse (colour pia te 133 , c) at first
colo urless becomin g ora nge in age with a green cast sho wing throu gh ; pe nicilli
cssentially as described aboye .
T he species description is ce nte re d upon th e typ e stra in CBS 29 1.48 = C M I
:19 ,752 = AT C C 10,104 = N R R L 795 = I FO 8, 137 = Q M 7,483, isolat ed by C h.
Thom from Fren ch Came m be rt cheese a t Storrs, Con necticut, U. S .A . in
1905. T he cultu re has rema ined remarkably sta ble during more th an 7S years
that it has been under labor atory cultivation .
Penicillium atramentosum does not appear to be wid ely d istributed in natu rc
out re prese nta tives 01' the spec ies are occasionally isolat ed from soil.
134 . Penicillium fe nnelliae Stolk: fig. 134 , colour plat e 134 , and SEM
~(
I.~
1
te
1,
tM
,
~
u..w..4:J..J
10
jrrn
I/?
fi< (Ji
~~ \?)
<\l>: !6~~
/?.
Stolk's original diagnosis follows, since this adequately presents the species in
question when first isolated:
'Colonies on Czapek agar growing restrictedly, attaining a diameter of 30 mm
within 2 weeks at 25C, consisting of a thin, buff to orange, ultimately pinkish basal
felt bearing abundant conidial structures, velvety, azonate , commonly buckled in
central areas and furrowed in a radial pattern, sporulating throughout the colony
but heaviest in submarginal areas. Conidiaf areas showing blue-green shades
approximating artemisia green, with the very narrow growing margin light celandine
green (Ridgway, PI. 47); when young surrounded by small orange zone, approximating orange cinnamon (Ridgway, PI. 29), consisting of submerged vegetative
mycelium studded with orange granules. Sectors which sporulate less abundantly
show orange brown shades approximating cinnamon when young, becoming
pinkish near Iight corinthian red with age (Ridgway, Pis. 29, 27). No macroscopically visible exudate drops are produced. Reverses of colonies showing orange
brown colours, ranglng fram cinnaman-rufous at tho margln thraugh hay's russet to
hnornat ite red in the centre, becaming dark purpla near hay's marcan with age
(Rldgway, Pis. 14, 27, 13) with the surroundlng agar caloured in Iighter tints of the
lino shades but at length becoming intensely coloured.
Colonies on malt agar growing more rapidly, attaining a diameter of 55-60 mm
w lthin 2 weeks at 25C, plane, velvety, very thin, azonate; conidial structures
produced throughout the culture but generally in greater abundance in central than
In marginal areas, showing blue-green shades near artemisia green (Ridgway, PI.
47) against a background of the orange coloured, largely submerged vegetative
mycelium. Marginal zone broad, showing conspicuous orange colours near
i lnnarnon-ruf o us (Ridgway, PI. 14). and consisting of radiating hyphae with the
jreen conidia l areas following the hyphae in unevenly radiating lines. Exudate
luckinq. Reverse of colonies show orange shades near zinc orange and cinnamonrufous (Ridgway, Pis. 15, 14).
Vegetative hyphae rather coarse, studded with orange granules uneven in diameter, 4.5-6.5 .m wide, often showing conspicuous swellings, especially on malt
Iga r. Conidiophores arising from the basal felt as well as directly from the substrat um, hyaline, septate, smooth-walled and fairly long, ranging from 250-500 .m
in length by 2.5-4.5 .m in diameter. Penicilli typically biverticillate and asymmetric,
vmying in complexity, but usually showing one or very rarely two rami in addition to
the main axis, each bearing like the main axis verticils of two to five metulae
torminating in clusters of phialides. Occasionally metulae and phialides arise from
the same level. Branched metulae may occur. Smaller heads, only consisting of a
verticil of metulae with phialides are also presento AII elements of the penicillus are
slight ly appressed, hyaline and smooth walled. Rami variable in length, ranging
from 14-35 .m by 2.5-4 .m in diameter, usually slightly enlarged at the apex up to
3- 5 .m in diameter. Metulae measuring 10-16 .m by 2.5-4 .m, enlarged at the
apex to 3.5-5.5 .m in diameter. Occasionally secondary metulae develop agreeing
in shape with the primary ones. Phialides occurring in fairly compact clusters of four
to six, with their axes roughly parallel, consisting of a large nearly cylindrical basal
part tapering distally to a small conidium -bearing tube (neck). Phialides usually
measuring 9-14 .m by 2-3 .m , occasionally larger up to 20 .m in length. Conidia
varying from cylindrical to ellipsoid with their bases sometimes flattened, hyaline,
extremely variable in size, commonly 3.5-5 .m by 1.5-3.0 .m, but occasionally
larger up to 10 .m, showing finely roughened walls and producing tangled chains
up to 150 .m in length. Neither ascocarps nor sclerotia were observed.'
Our not es follow:
Colonies u po n CA (colour plat e 134, A and a) in our observatio n co nform
Iairly well with Stolk's orig inal d iagnosis ei the r in rat e of growth or in ge neral
cultural characte ristics and in detail s of morpho logy (fig. 134, A, B and C), bu t
with conid ia co ns isten tly larger , ranging u sually from 5-7 tut: by 2.5 -4 .5 tut ,
co n sp icu o u sly spinulose when obscrvcd lindel' th e light mi croscope (Ii g. 1:H ,
B) and granular wh cn ohscrvcd undcr th c S EM (plat c :,n , IOp: righr).
Colonies upon CYA (colour plate 134, A and a) growing fast cr, auaining a
diameter of 45 mm in 14 days at room temperature (20-25C), con sisting 01' a
clos e-textured basal orange felt, typically velv ety, strongly wrinkled in a radial
pattern, conspicuously zanate and umbonate in the centre; with thin margin
surrounded by a fringe of deep orange-red coloured submerged hyphae
ext ending about 1 mm beyond the aerial growth ; sporing abundantly in central
areas in deep blue-gray shades (Sguy, PI. XXXI, 461) in conspicuous zoncs
with th e ripening of conidia, remaining persistently orange elsewhere; exudate
and odour lacking; reverse of colonies (colour piate 134, b) developing very
dark purple-red vinaceous shades ; similarly coloured pigment slowly diffusing
into the surrounding agar; penicilli essentially as described aboye .
Colonies upon MEA (colour piate 134, C and c) in our observation are
duplicating those described in the original diagnosis and heavily sporing
throughout in blue-green shades (Sguy , PI. XXX, 437, 438).
The species description is centered upon the type strain CBS 711.68 = CMI
151,747 = ATCC 22,050 = IJFM 7120, isolated by DI'. R . Bouillenne , Institut
Botanique, Liege, Belgium from Congolese soil in 1956 . It is at present known
only from the type species .
Penicillium Jennelliae appears to be most satisfactorily assignable to the
Asymmetrica-velutina subsection near the P. oxalicum series , on account of its
typically biverticillate and asymmetrical penicilli and its velvety cultural
appearance . It clearly differs from both P. oxalicum Currie et Thom and P.
atramentosum Thom in the shape , size and roughness of its conidia and in its
cultural appearance .
lo uid iop ho rc s coa rsc, co m para tively sho rt , ari sin g lrom su b mc rgc d hyphac
111'
Pcnicilli asymmctric, very irregular in siz e and pau crn, co rn para tivc ly vcry
IlII'ge, with id cntity 01' branchcs ami m ctulue olt cn poorly csta blishc d , bcaring
phialidcs a nd con id ial cha ins al vari ou s lcv els in th e co n id iopho re .
Co n id ia a t Iirst cy lind rical, usu all y becoming clliptical 01' occasionall y su b~ l () l>o sc , extre mcly variable in size but larger than in a ny othe r ser ies.
'I'h c seri es is ac tually represented by two sp ecie s, Penicillium digitatum
Saccard o a nd P. j aponicum Smith . The former is we ll kn own 1'01' being responsib lc 1'01' ser iou s losses 01' citrus fruit s in com me rce. In its natural habitats , thi s
mould produces a n olive-green colou red rot th a t ca n gene rally b e easily
disl in guished from th e 'blue-green rot ' 01' citrus fru its produced by m embers 01'
lite Penicillium italicum series. The latter spec ies isol at ed a nd fir st d escribed b y
Al: without indicating its origin is known only from th e type strain . It
rx -scmble s P. digitatum only in th e size a nd th e shape 01' th e co n idia .
lu..u..W..uJ
10 }lm
\J
c:000
) o
fR .
Peniclllium digit8tum Saccardo - Sylloge Fungorum, Vol. IV: 78. 1886. Sea also
Ch. Thom, Bull . Bur. Anim . Ind. U.S. Dep. Agric. 118: 31-33. 1910; also Ch.
Thom, 'The Penicillia', pp. 242-245. 1930; also Raper and Thom, 'A Manual of the
Penicillia', pp. 386-390. 1949.
Synonym
Coloni es upon C A (colour plat e 135, A) grow ing ve ry res trictedly, from
punctiform to 10 mm in di ameter in 14 days at room temperature , consisting
mo stly of subme rged vegetative mycelium from whi ch a few conidial stru ctures
a rise of sm all dimen sions and irregular pattern , presenting without any doubt
th e gen eral aspec t of moulds suffering from sorn e typ e of nutritional deficiency
(a vitamin deficiency in th is particul ar case).
Colonies up on C YA (colour plate 135 , B a nd b) growing lu xuriantly ,
broadly spreadi ng, attaining a diameter of abo u t 40 mm in 14 day s at room
temperature (20-25 C) , cons ist ing of a den sely textured basal felt, typically
velve ty, flat in submarg inal and m argin al a reas and irregul arI y rai sed ancl
wrinkled in th e centre in a crateriform pattern , with undulating thin margin
surrounde d by a frin ge of sub me rge d hyphae extending abo ut 5 mm beyond
the ae rial gro wth ; heavily sporing th roughout in yellow- green to olive green
shad es (Sguy, PI. XXV , 373), show ing sorne zonation; exudate lacking;
odo ur pronounced , aromatic; no colou red pigment diffusing in to th e su rrou nding ag ar; colony reverse (colour plate 135, b) developing redd ish brown
shades; pe nicilli cha racte rized by great irregul arity in th e number and
dimension s of parts (fig. 135, A), usu all y cons isting of an indefinite number of
bran ch es a nd/or m etulae su ppo rting a limited but va riable seri es of phi alides,
eac h producing a cha in of co nidia that a re typi call y elliptical bu t showing wide
individual variation ; con idio pho res very sho rt , com mo nly rangin g from 30 to
100 Jlm in length by 4. 5-6 Jlm in di am eter, smooth walled , arising from
submerged hyphae or from th e basal fe1t; penicilli typi call y biv erti cillate
asym me trica l but va ry ing greatly in dimen sion s and in com plexity, with
branch es, metulae and phialides often poorI y defin ed ; cons isting of bran ches
vari abl e in len gth , bearing eithe r metulae and ph ialides, or phialides alone;
metul ae variabl e , com mo nly rangin g from 15- 35 Jlm by 5-7 uit, and bearing
small clu sters of phi alides eq ually va riable, rangin g from 10 to 30 Jlm by 4- 5
iuu , more or less cylindrical or bottle shap ed, terminating to a broad en ed sho rt
neck ; con idia at first cylindrical, becoming elliptical, sm ooth and thick wa lled
(fig. 135 , B, and SEM plat e 23, centre: left ), va ryi ng greatly in shape and
,J I I
I'I'OIll
:L5 lo 5.0 un by
1.11 -6 .0 .t lll , and occasion ally up to 10-1 2 .tlll by 6 -8 .tm ; conid ial cha ins
v/ll'ying grea tly in lcn gth , up lo 150 .tlll , usuull y di vergent a nd com mo nly
I w('(lllling cnta ng lcd with other cha ins Irom [he sa rne on adjacen t pen icilli.
Colonies upon M EA (colour plat e 135, C an d c) sp read ing rapidly, attaining
1 d iamctcr of 40 -60 mm in 14 da ys a t room ternperature (20 - 25C ), cons isl ill f.{ 0 1' a looser textured basal felt , thin , flat , not fu rrowed , with very thin ,
irrc gula rly di ssected m argin surrau nde d by a fringe of su bmerged hyphae
l'xlc ndi ng abo u t 4 mm beyond the ac rial growth ; heavily spo ring throu gh out ,
uppoa rin g den sely velve ty ; conid ial areas developing pale yellow-green shades
(SI;gUY, PI. XXV , 366); cxudate lacking ; odour pronounced , a ro matic ; no
I'II lourcd pigment d iffus ing int o th e surroundin g agar; colony reverse (colour
plat c 135, e) uncoloured 01' in d ull tan shade s with a pale grc en ca st showing
hrou gh: pen icilli essentially a s describe d aboy e .
T he spe cies description is cc nte red up on strain C BS 3 19.48 = I MI 39, 748 =
"TCC 10,030 = NRRL 786 = I F a 7,006 = Q M 7,552 = IJ FM 722 1, isolat ed by
( :11 . Thom in 1904 at Storrs, C onnecticu t , USA , fro m Citrus sp . , and
nume rous strains mor e rccently isolated by th e author in Spain, from Citrus
uurantium Ri sso , Cit rus vulgaris Ri sso , Citrus limonium Risso , Citrus medica Risso ,
a lid from Citrus deliciosus Risso .
T he description as presented ade quately covers all but a n occasion aI
in cmber of thi s spec ies a s th ey a re isolated from fruits collected frorn packing
plants, sto rage wareh ous es, a nd market s. OccasionaI variants have been
r-ncountered . O ne suc h form producin g wh ite conid ia and showing no green
ro lour was isolat ed by DI'. H .S . Fawcet t, Ri verside , C aliforn ia , U SA in 1924 ,
.md was citc d in Thom ' s M onogr aph ( 1930 , p . 245) as a new va riety ,
l 'enicillium digit atum Saccardo va l'. calijornicum Thom . It has been reported as
dcstructive of ora nge s in th e u sual olive green form . The strain was soon lost
lrom Thom ' s collection and ha s not again bee n rep orted . T he occ urre nce of
white 0 1' ta n varia nts has been obs erved in m any spec ies, and the strain upon
which Thom based his va riety was undoubtedl y of such or igin o
136 . PenicilliumJaponicum Smith : fig . 136 . colou r plat e 136, and SEM
:17B
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'Colonies on Czapek agar spreading rapidly and broadly, velvety, rather pale bluegreen, with a very broad margin consisting of a loose web of interlacing hyphae,
almost white; reverse reddish brown except that there is often a central spot which
is almost colourless; penicilli typically asymmetric, either consisting of a compact
cluster of metulae and phialides or having two to several rami bearing metulae, or
occasionally very large and complex with four stages of branching, the totallength
being 50-80 p'm; conidiophores mostly arising from the submerged mycelium,
smooth, often sinuous, 3-4 p.m in diameter; metulae variable but mostly 10-16 p.m
by 3-4 p.m; phialides remarkably uniform in length, 12 p.m by 2.8-3.0 p.m; conidia
very variable in size and shape, mostly ovoid to elongate, occasionally pyriform,
smooth or with a slightly uneven surface, 3.5-7 p.m by 3-4 p.m, in chains, roughly
parallel at first, then tangled.
The varietal epithet proposed by Abe refened to the spreading growth of the
isolate . However, P. digitatum, on a suitable culture medium, spreads extremely
rapidly, so the epithet is an unfortunate one. More serious is the fact that the variety
resembles P. digitatum in the size and shape of the conidia. It differs in surface
;17!}
i olour. reverse col our, and ty pe of penicillu s, also in it s ability to grow well on
ipe k agar. It is difficult to prepare slides of P. digitatum, the penicilli being frag ile
'II1d rarely found unbroken in f luid mounts. P. jeponicum , on the cont rary, is easy to
rnoun t, and , provided that the culture is not too old , readily gives slides showing
ores of intact penicilli . The varietal epithet used by Abe is unsuitable as a specific
oplthet , so it has been cons idered adv isable to provide a new name for the fungus.'
.J O\ I
dense nciwork 01' white Iloccosc vegetativ o hyph ac influ cn cin g gr eally th c
colony appearance ; with margin a ppearing gra n ular owing to thc production
of abu ndant synnem at a ; colony su r face sulcated by sha llow radial f'urrows a nd
slightly rai sed in ce ntral areas; heavily spo ring throughoul in pale blu c-gr ccn
sha des to blue-gray tints (Sgu y , PIs XXIX, 435, and XXX , 449) ; ex uda re
and odo u r lacking; dark a m ber colou re d pigm en t diffusin g int o the
su rrou nd ing agar; colony reverse (colour plate 136, b) stro ng ly pulling the ag ar
away fram th e cult ure dish a nd cracking it , dev eloping deep reddish brown
sha des; pe nicil1i esse ntially as described by G . Sm ith but with all elernents
la rger ; con id iopho res m ostly a risin g from the su bs tratum, m ore 0 1' lcss
ag gregated into erec t bundles 0 1' synnemat a , parti cul arl y cons picuous in
margin , e1sewh ere th ey are obscu red by th e den se ove rg rowth of white floccose
hyphae , measuring up to 200 tm in len gth by 4-6 uit: in di amet er; branchcs
whe n present m ostl y 20- 40 utt: by 4-5 tm ; m etul ae variable but m ostly 20 -25
tm by 3 - 5 tm ; phialides mostly 15- 18 tm by 3.5 - 5.O iut , tap ering to a
conspicuo us ly long narrowed neck; con idi a varia ble in size, usuall y strang ly
elliptical , m ostly rangin g from 4 .5 to 9.0 tm by 3.0 -4 .5 tut , srnoo th-wa lled or
nearl y so (fig. 136, B, and SE M plat e 23, ce ntre: right) ; con id ial chains at first
rou ghly parall el (fig . 136 , A) , th en becomin g tangled (fig . 136 , C) .
C olonies on M EA (colou r plate 136 , C and c) gro wing rapidly a nd braadly,
attaining a di ameter of 65 mm in 14 days at raom te m pe rature (20 -25C ),
cons isting of a loos er textu red b asal felt , flat, not fu rrowed, with wid e a nd thin
m argin su rrau nde d by a frin ge of reddish brown-coloured , su bmerged h yphae
exte nd in g m ore th an 5 mm beyond th e ae rial gra wth, cons picuo us ly zo na te,
with colony surface appearing gra n ula r owing to th e numeraus fas cicles 01'
synnem at a arrange d in co ncentric zones in su bmarg inal a nd m arginal areas,
with ce ntre ove rlai d by a loosely wov en netw ork of floccose h yphae lend ing a
lanat e appearance to it ; heavily sporing throu gh out in gray-green shades
(Sguy, PI. XXX, 449) with fascicles most abu ndantly praduced along th e
int ercolon y m argin s; od our strong, suggesti ng fennel (A nethum f oeniculum L.) ;
exu da te la cking ; no coloured pigment diffu sing in to the surra u nd ing a ga r ;
colony reverse developing deep reddish brown shades (colour plat e 136, e);
peni cilli (fig . 136 , A) esse ntially as described aboye .
The species description is ce nte re d upon th e type stra in C BS 32 7.59 = C M I
68 ,233 = ATC C 18 ,317 = IFO 5, 747 = Q M 7,298 = IJFM 7129 , isolated by S .
Ab e , prabably fram soil , J apan , 1956 . It is known only fram the typ e stra in .
Penicillium japonicum Sm ith, as received fram th e Centraalbu reau voor
Schimmelcultures, Baarn , The Netherl ands, in 1979 , ac tu ally exhib its noteworthy va riation away frorn th e original, p articul arly the restricted grawth a nd
poor sporulation upon C zape k's agar culture m ed ium (colou r pla te 136, A) ,
prob abl y due to a vitam in defi ciency suffered by th e strain in question al't er a
long peri od of lab oratory cultiva tion, as it ca n c1early be inferred from th e
lu xuri ant gro wth ami ubu nda n t sp orulation upon C za pck ' a gar plus a dr lcd
viuu nins (as Difco yca st cx trac t) (SCl: (colour plat c 136, B) . Furthermorc, in
11111' obse rva tio ns , P. [aponicum grow n UpOll malt ex trae r agar develops a strong
1I 1ld rha rac ter istic odour suggesting fenncl and whcn ~rown upon CY A and
M EA (colour plate 136, B and C) produces abundant fasciculation, features
111)1 observed by previous authors .
A close relationship between Penicillium japonicum Smith and P. digitatum
Saccardo is not presumed here, and they are con sidered together primarily as a
u uutcr of eonvenienee. They do , however, show eertain features in eommon,
p.ut icularly the eomparatively large asymmetrie eonidial struetures and the
sha pe and size of conidia . However, in our opinion, the produetion of
xyn nc m a ta suggestive of a eertain relationship to speeies included within the
Asym me triea - faseieu lata sub-seetion should not be overlooked .
.) 0
. . . . 391
.... 385
. . . . 389
.... 387
The Penicillium roquejorti series a s u nderstood by Raper and Thom ( 1949) was
represented by two species, namely , Penicillium roquejorti Thom a nd P. casei
Staub, whi ch is now regarded as synonymou s with P. verrucosum D ierckx , va ro
cyclopium (Westling) Samson , Stolk et H adlok (Samson et al. 19 76).
In the present study, the series has been broadened enough in concept to
accomodate four additional forms as follows: Penicillium jagi Martnez el
R amrez, P. cordubense Ramrez et Martnez , P. mali Novobranova, and P.
jarinosum Novobranova .
The principal species of th e series, P. roquejorti Thom has been studied
particularly with relation to its role in cheese manufacture . lt is the
predom inant mould in the who le group of cheeses charac te rized by streaks, or
'marbling ' , of green mo ulds . C losel y related strains of thi s form appear in thc
true Roquefort , or sheep's m ilk chee se of sou the rn F rance, in the ' from ages
bleus ' , or blu e cheeses of cen tr al France, m ade either from sheep' s m ilk or
cow ' s mi lk , in th e Gorgonzola che ese from northern Italy, in th e Stilton ch eese
as made in England, in the Gammelost cheese as made in Norway, in the Bluc
chee se as made in D enmark , or th e American Roquefort cheese as made in the
US , and in many varicti es of loose-textu red che eses whi ch obtain their
charac teristic flavour from th e moulds which invade th e ha les and crac ks
throughout th eir mass .
A clas e relationship between Penicillium roquejorti Thom a nd the four
additional sp ecies incl uded now in th e series is not presumed , and th ey are
considered together primarily as a matter of con ve nience. They do, however,
show certa in characte ristics in com m on , particu la rly th e co nspicuou sly roughwa lled conidiophores, th e co m m only thin arac h noid margins, particularly
upon malt extract agar , th e dark green colou rs develop ed in th e colony surface
and in th e rev erse , and th e globose, thi ck walled and smooth conidia .
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Penicillium roqueforti Thom - Bull . Bur . Anim . Ind . U.S. Dep. Agre. 82: 35-36.
1906. Also Ch. Thorn, ibid. , 118: 34. 1910; also Ch . Thom , 'The Penicillia ', pp .
277-279. 1930; also Raper and Thom, 'A Manual of the Penicill ia' , pp. 395-401.
1949.
Synonym ies
Penicillium aromaticum Sopp - Monograph ie, pp. 159-161. 1912. See also Raper
and Thorn, ' A Manual of the Penicillia' , p. 400. 1949.
Penicillium etro-viride Dierekx - Ann. Soe. Sci. Bruxelles . 25: 87. 1901. See also
Biourge's Monographie, La Cellule . 33: 199. 1923; also Raper and Thom , 'A
Manual of the Penicillia ', p. 399. 1949.
Penicillium etro-viridum Sopp - Monographie, pp. 149-150. 1912. See also Raper
and Thorn, ' A Manual of the Penicill ia.' p . 400. 1949.
Penicillium biourgeii Arnaudi - BolI. 1st. Sieroterapieo Milanese. 6: 25-27. 1927;
also Zentbl. Bakt. Parasitenk. Inf. Hyg. 11 Abt. 73: 321-330. 1928; also Raper and
Thom , ' A Manual of the Penicillia' , p. 401. 1949.
whit c acrinl hypha c pnrtially obs curing the oth crwisc hcavily sporin g co nid ia l
ureus; co n id ia l arcas d cv cloping gray -grccn sh ades with th c ripcnin g 01' conidia
(Sg llY, PI. XXIX, 4:n); exuda re limir cd in amount , as minute colou rless
droplcts cnrnc shed in th e my cclial fclt ; odour lacking; no colou re d pigment
dilTusing into th e su rrou nd ing agar ; colony rcvcrs e (colour plat e 137 , b)
ilevcloping brown to lilacinum shades in th e cen tre, almost colou rless in th e
margin ; peni cilli essentially as described abuve .
C olun ies upon MEA (colour plate 137 , C a nd c) attain ing a di ameter of 65
111m in 14 days (20 -2 5C), consisting of a very thin loose-textured basal felt ,
iypically velvet y , with a very wid e arachnoid margin, largely sub m er ged and
uxtcnd ing abou t 10 mm beyond th e aeri al growth ; colony surface flat , not
urrowed ; heavily spo rin g throughout in blue-green shade s (Sguy , PI.
XXVII , 403); exudat e and odo ur lacking; no coloured pigment diffu sin g into
lile surro und in g agar; colony reverse (colour plate 137, e), at first colou rle ss
with a gree n cast showing through , blackening in age; conid ial struc tures
usually exh ib iting increas ed roughness and con id ial chains tending to form
\on ger a nd more com pa ct colu m n s (fig. 137, C) , up to 300 tut: in len gth .
Species description is cen tere d upon th e typ e strain CBS 221. 30 = CMI24, 31 3
= ATCC 10,110 = ATCC 1,129 = NRRL 849 = IF0 5,459 = QM 1,9 37 = IJFM
7201, isolated in 1904 from french R oquefort chee se by Ch . Thom , and from
nurnerous other stra ins possessing similar cultu ra l a nd morphological characleri stics that hav e been isolat ed by th e author from different sub strata . Among
such cult ure s now m aintained in our collection strain IJFM 3706, isolated by th e
au tho r in 1976 from Cabralc s chee se of no rthe rn Spain , made from cow's milk ,
ma y be m entioned and regarded as entire ly typical of th e species as it is
understo od by R aper and Thom (194 9). (Colour plates 239 and 240 present
lwo representati ve strain s of P. roqueJorti, isolated by th e autho r from Cabrales
cheese a nd from th e air , respectively .)
138 . Penicilliumfagi Martnez el Ramrez : fig. 138, Colour plate 138, and
SEM plate 23, (bottom: right).
Penici/lium fagi Martnez et Ramrez - Mycopathologia . 63: 57-59. 1978.
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nxcs rou ghly parall cl, a bo u t 7-10 J.t11l b y 2.:i -:L:i ,tlll , tapcrin g abruptl y l o a
sho rt narrowcd ncck ; co n id ia co m pa ra tivc ly vc ry small , 2 -2 .5 ,tm in di amct cr ,
globo sc, with wall s srnoot h 01' ncarl y so ( lig. 138 , B, a nd SEM pl at e 23,
ho uo rn: r igh t); eo n id ia l chains loosel y parall el beco m ing tangled in age (fig .
l :lS , C) .
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textured b asal felt , typically ve lve ty , consp icuo u sly zonate, radiately su lcated
with shallow furrows, and with centre rais ed; with thin undulating margin
surrounded by a fringe of submerged hyphae ext ending about 2 mm beyond
th e aeri al gro wth; with colony surface ov erl aid by a thin , closel y wov en network of ae rial hyphae influencing greatl y the colony appe a ra nce ; heavily
spo ring in blue-green shades (Sguy, PI. XXVII , 402) with con id ial areas
partially obscured by th e thin overgrowth of aerial hyphae shading to
ochraceou s tints; exudate abundantly produced, as large dark amber- coloured
drops ; od our faint , ' m ouldy ' ; no coloured pigment diffusing into th e
surrounding ag ar; colony rev ers e in reddish brown shade s with a gree n cas t
showing through (colour plat e 139, a); penicilli (fig. 139, A) typically
bi verticillate asym me tric, charac teristically de veloped in a dense and eve n
stand to produce a velvet y effect, variously ramified , with con id iophores
/
conspicu ous ly spinulose, arising primarily from th e su bstra tum, consisting of
two or three sta ge branched struc tures ; con id iophores short up to 150 p.m in
len gth by 2.5-3.5 p.m in di ameter; rami ranging from 15 to 20 p.m by 3.0-4 .0
p.u , some what appressed , rou gh wall ed ; m etulae more or less cylind rica l,
. J U ;J
usually rough wall cd, mosrly 7 -14J.ull by :1.O - !Ul/UII with a p iccs c n la rged in a
vcsicle up to 4 -6 1t1ll in di um et cr, smoo th-wa lled metulae are occasionally
c nco un te red (fig. 139, A); phialidcs bottlc sh aped, 7-121tm by 2.7 -4.0 1tm,
lapering abruptly to a short-pointcd nc ck; co n id ia globose to subglobose ,
~ . 8 - 4 . 0 tut: in long axis, smooth wall ed 01' nc arly so (fig. 139 , B, and SEM
plato 24, top: left) ; co n id ia l cha in s tending to produce long, loosely parallel ,
c1i vergent colu m n s (fig. 139, c).
Colonies upon CYA (colour plate 139, B a n d b) growing m ore rapidly than
aboye, attaining a diameter of a bo ut 50 mm in 14 days at room temperature
(20- 25C ), co ns istin g of a c1ose-textured felt , typically velv ety , stronglyand
dcepl y wrinkled in a radial p attern , with ce n tre raised and fold ed in a
cra ter ifo rrn pattern , with thin , flat , undulatin g m argin surrounded by a frin ge
01' su bmer ged hyphae extending about 3 mm beyond th e aerial growth; very
heavily sporin g throughout in deep blue-green shades (Sguy , PI. XXX , 447)
with the ripening of con id ia, show in g ochra ceous shades in ce n tr al areas;
odour faint , indefinite ; exu da te limited in amount, as minute a m be r coloured
droplets enmeshed in th e m yeelial felt ; no colou re d pigment diffusing into th e
su rrou nd in g aga r ; colony reverse (colour pl at e 139 , b) developing reddish
cin na m on shades; penicilli esse n tia lly as d escribed aboye .
Colonies upon MEA (colour pi ate 139 , C and c) attaining a diameter of
a bou t 45 mm in 14 days at room temperature (20 - 25 C ) , con sistin g of a looser
tex tu red bas al felt, a p pea r ing vel vet y to powdery in heavil y spor in g areas , with
margin thin , a rac h noid, largely submerged a n d extending up to 5 mm b eyond
(he . a erial growth ; heavily sporing throughout in deep blue-green shades
(Sgu y , PI. XXX , 447) ; exu d a te and odour a b se n t ; colony su r fac e flat , not
Iurrowed; no colou red pi gment diffusing into th e surrounding a ga r; colony
rev ers e (col our pl ate 139 , e) developing very d ark reddish brown shades;
penieilli ess entially as des cribed a boye.
The sp ecies description is ce n te re d upon th e type strain IJFM 7030 = ATCC
42,238, isolated by th e author of the current stu d y from sand y soil, Madrid,
Spain, M ay 1979 . It is at present known only from th e type strain .
Penicillium cordubense is a distinctive species ch a rac te rized by its typically
velvety colo n ies, with ara chnoid margins, especially upon MEA , b y th e very
dark-coloured reverse upon MEA and its co n sp icuo u sly rough-walled
con id iopho re s. The correet plaeement of this sp eeies appears th en , to b e m ost
sa tisfae tor ily ass ign able to th e P. roqueforti series in the Asymmetriea-velutina
sub -seetio n .
140 . Penicillium mali N ovobranova: fig . 140 , colou r pIate 140 , and SEM
pIate 24 , (top: right).
Penici/lium mali Navabranava - Bial. Nauki. 15: 103-108. 1972.
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C olonies upon C A (colou r plat e 140 , A and a) att a ining a di amet er of abo u t
40 mm in 14 days at room temperature (20 -25 C) , cons isting of a rather loo setextured basal felt , typi cal1y velv ety , som ewhat zanat e, flat , with white undulatin g m argin surrounded by a narrow fringe of su bme rge d hyphae exte nd ing
abo u t 1 mm beyond th e aerial gro wth; heavily sporing throu gh out in deep
gre en sha de s (Sgu y , PI. XXVII , 401); with th e rip ening of conid ia , pa ssin g
through va r ious shades of blue-gr een (Sguy , PI. XXX , 446 , 447); odo ur
faint , ' mouldy ' , exudate abu ndantly produced , as dark am ber drop s; red dish
brown-coloured pi gm ent diffu sin g into th e surrou nd ing agar; colony rever se
(colour piate 140 a) in pale cin namo n to reddish brown shades; peni cil1i (fig.
140 , A) typi cal1y bivertic il1ate asymm etrical , cons isti ng of ve rticils of ram i a nd
metulae or verticils of m etulae alone, m or e or less appressed; con id iopho res
mostly a rising fro m th e bas al felt in a den se and eve n stand, com mo nly sho rt
up to 100-1 50 ,tm in len gth by 3- 4 ,tm in diameter , septa te, wit h wa l1s
conspicuo us ly a nd fine1 y spi n ulose , with apices enla rge d in a vesicl e up to
4.5 - 6 .0 ,tm in di ameter; rami wh en present in number of on e or two eleme nts
in th e vert icil, u su al1y 18-25 ,tm by 3 .5 tui , cons picuo us ly spinulose;metul ae
lormiu g who rls 01' 2- :i clc mc n ts in lhe verticil , lIIos11 y 11 -1 511-111 by :i.O - 1'.5
unv , wi th pi ces c n la rgc d in a vesiclc up l o :i.5 -5.0 tit u in di amet cr , wi th wall s
lind y spinulosc ; phi alid cs ve ry regul ar in sizc, bottl e sha pe d, com mo nly 8- 10
Itlll by 3. 5 - 4.0 1I-m, tapcring grad ua lly to a n inconspi cu ou s, com paratively
widc neck ; co n id ia globose to subglobo se, smooth walled or nearl y so whe n
observed under th e light mi cr oscop e (fig . 140, B), bu t delicately gra n ula r when
observed under the SE M (pl ate 24 , top: righ t), m ostly 4-5 11-m by 3 .5 - 4.5 11-m
in di ameter; co nid ial chains te ndi ng to adhe re in to loos ely parallel divergen t
colu m ns (fig . 140 , C).
Colonies upon C YA (colour plat e 140 , B a nd b) atta in ing a d iameter 01' 45
mm in 14 days at roo m temperature (20 -25 C), consis ting 01' a close r textured
basal felt, ve lve ty, a ppearin g finel y granu la r, stro ngly and deepl y sulca ted by
nu rnerou s radial fu rro ws and slightly raised towards th e ce nt re; with sorne
tloccose tufts 01' white ae rial h yph ae in th e colony cent re; with a th in wh ite
rna rgin su rrou nde d by a fringe 01' su bme rge d hyphae exte nd ing abo u t 1 m m
beyond th e ae rial growth; heavily spo ring th rou gh out in di fferent shades 01'
brown- gr een to deep gree n (Sguy, PIs XXI , 3 14, and X X VI , 386) with the
ripening 01' co nid ia ; odo u r suggesting Actinomycetes; exudate limited in
amount, enmeshed in th e m ycelial felt; pale amber-coloured pi gm ent d iffusing
into th e surrounding aga r; colony reverse (colour plate 140 , b) in reddish
cinnamon shades; peni cilli esse nt ially as described aboye.
Colonies upon M EA (co lou r plate 140 , C and c) attaining a di ameter 01'
abo ut 50 mm in 14 days at room temperature (20- 25 C), consisting 01' a loosetext ured basal fel t , appearing velvety to delicately gra n ula r, flat, thin , with
ma rgin ar achnoid largely submerged and exte nd ing abou t 3 mm beyond the
aerial growth, wit h small sectors 01' floccose wh ite ae rial hyphae u su ally
ove rlaying th e colony centre ; heavily spo ring throu gh out in yellow -green
sha des (Sg uy, PI. XXVI , 386); odo u r lacking; ex udate limited in a mo u nt, as
m in ute colourless d roplet s em be dde d in m ycelial felt ; no co loured pigm ent
d iffus ing into the surrounding agar; colony reverse (colou r plat e 140 , c) at first
colourless, th en blackening in age; peni cilli esse n tially as described ab oye but
with conid ial chains tendin g to produce m ore com pact co lumns,
T he spec ies description is ce nte red up on th e typ e strain CBS 500. 73 = VKM
F-1 ,751 = ATCC 24,72 7 = IJ FM 7130 , isolated from Pirus malus L. fruit, in 1964
by 1'.1. N ovobranova , Institu te 01' Pomology and Ampelogr aphy , SS R 01'
Kazachstan , in the Alma- At a region, U SSR .
Penicillium mali N ovobranova is a dis tinctive spec ies whi ch , in ou r opinion
appears to be m ost satisfac torily ass igna ble to the P. roqueforti series on accou nt
01' its cultu ra l characterist ics a nd detail s 01' m orphology .
141. Penicillium f arinosum N ovob ra n ova: fig . 141 , colo ur pl ate 141 , a n d
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Fig. 141. Camera lucida
drawings of Penicillium
farinosum Novobranova ,
CBS 499.73. A, Detailed
drawings of the penicilli . B,
Mature conidia . C, Habit
sketches .
Penicillium farinosum Novobranova - Nov. Syst. Niz. Rast. 11: 232-233. 1974.
Novobranova's original description follows :
'Colonies upon Czapek's agar attaining a diameter of about 30-40 mm in 10-12
days , consisting of a loose -textured felt appea ring pruinose or flour Iike at the
colony surface, which is gray-green coloured. Colony reverse uncoloured . Exudate
lacking. Odour very pronounced , 'mouldy'. Penicilli typically asymmetric, with
conidial chains tangled . Conidiophores coarsely roughened , 200-300 p.m by 4.5-5.6
(6.0) p.m, rami roughened , 12.0-18.0 by 3.5-4.0 p. m, metulae in whorls of 3-4
elements in the verticil , 10-12 p'm by 3.2-3.5 p. m, phialides 6.0 -8.0 p'm by 2.2 -2.5
p. m . Conidia smooth walled , globose to subglobose, 3.5-4.0 p.m in diameter. It
belongs to the Asymmetrica section . Related species unknown.'
(Translated from the Russian by Prof . L. Vilas) ,
Colonies u pon CA (co lour plate 141 , A and a) growing rather restrictedly,
attaining a diamet er of about 25 mm in 14 days at room temperature , velvety
in appearance, almost flat , not furrowed, or sligh tly fold ed in th e cen tre,
cons isling 0 1' a dense busal Iclt , with whitc (hin murgin surro u ndcd by a ri ugc
0 1' sub me rged hyph ac extc nd ing up lo 1:> II1Ill bcy ond thc acri al gro wth ; colony
surlace ovcrla id in places by a loosc1y WOVl:n netw ork of Iloccose white aerial
hyphae , lending lo th c colony a lan at c appcara ncc ; heavily spo ring throu gh out
in dccp grcc n shadc s (Sguy, PI. X X XI, 46 1); odour pen ctra ting , ' mo uldy',
exud a re limited in amo u nt, as minut e dropl ets enrneshed in th e m ycelial felt;
110 coloured pigm ent d iffus ing int o th e su rround ing agar; colony reve rse
(co lour plat e 141 , a) in pal e cream sha des ; peni cilli typically asym me tric (fig.
141 , A), co ns isting mostly of verticils of 3- 6 m etulae and less fre que ntly of
hranc hcs and metul ae , with all ele ments exce pt the phialides coa rse ly tuberenla te 0 1' wa rty; conidio pho res lon g , up to 300 /l m 0 1' m or e in length by
'1.0 - 5.5 /lm in d iameter, slightly enlarged at apex up to 4.5 -6.5 /lm in di ameter; ra mi 15- 25 /lm by 4 .0 /lm ; m etulae more 01' less cylindrica l, 13- 23 /lm
by 3.5 -4 .5 /lm , in clusters of 3-6 01' m ore ele me nts in th e verticil, rath er
d osely appressed; phialides bottle shape d, taperin g in a m or e 0 1' less lon g and
na rr owed neck , commo nly 9- 13 /lm by 4. 0 -4 .8 /lm ; con id ia globose to su b globose, 3 .5 -5 .0 uit: in long ax is , with walls smooth wh en obse rve d under th e
light micr oscop e (fig. 141, B) and very finely gra n ula r wh en obse rve d under
the SE M (pla te 24, centre : left) ; con id ial chains tending to adhe re into long
loosely parallel colu m ns (fig. 141, C) up to 300 utt: in len gth .
Colonies upon C YA gro wing m or e rapidly and broadl y spread ing (colou r
plate 141 , B and b) a tta ining a di ameter of 60 mm in 14 days at room
tem perature (20 - 25 C) , cons isti ng of a loose-textured basal felt, some what
fur ro wed an d rai sed , appearing velvety , with m argin thi n and irregul arl y
di ssected , largely subme rge d , ex te ndi ng abou t 3 mm beyond the ae rial gro wth,
app earing some what arach no id; very heavily sporing throu gh out in blue-green
sha des (S guy , PI. XXXI , 46 1); exudate abu ndantly produced as crys ta l clear ,
un coloured drops ; odo ur stro n g, suggestive of the Beta vulgaris L. aroma; no
coloured pigment diffusin g into th e su rrou nd ing ag ar ; colony reverse (colour
plat e 141 , b) developi ng reddish bro wn shades wit h a green cast sho wing
thro ugh in m argin ; peni cilli essentially as descri bed aboye .
Colonies upon M EA (colour pIate 141 , C a nd e), spre ad ing broadl y ,
attaining a di ameter of 55 mm in 14 days at room temperature (20- 25 C),
consisting of a loose-t extured basal felt, typi call y velvet y , some what powdery
0 1' pr uinose, fla t , not fur rowed, with margin arach no id, largely sub me rged,
exte nd ing abo u t 3 mm beyond th e aerial gr ow th ; heavily spo ring throu ghout in
gree n sha des (Sguy, PI. XXVIII, 417); odo ur penetrating, aromati c; exudate
lacking; no coloured pigm ent di ffusin g into th e surro und ing agar; colony
reverse (colour pIat e 141 , e) in yellowish shade s with a gree n ca st .showing
th rou gh ; penicilli essentially as describe d aboye , with con id iopho res eve n
lon ger, m ostly arising primarily from th e su bs tratu m, and con idial chains
adhe ring in m ore com pact colu m ns .
"."1'
'I'hc spec ics dcscripti on is cc nrcrcd llpOIl rhc t y p c srrn in GIlS 199. 7 :~ - VI< M
F-1 ,746 = CMl 174 ,717 = ATCC 24 , ni = I.lFM 7 1:11, isolat cd from (he su rfac
0 1' 'Schasselas' u nd er sto rage, in Octo bc r 1967 , by T .I. Novobran ova ,
lnstitu te 0 1' Pomology a nd Ampelography , al' the SSR of Kazach st an , USSR ,
in th e Alma -Ata region . lt is kn own only fro m th e typ e st ra in.
Penicillium fari nosum N ovobranova , on accou n t 0 1' its cult u ra l charactc ristics
and details al' morp ho logy is beli eved to be m ost sa tisfac torily assign able to the
P. roqueforti series.
Penicillium brevi-compactum se r ies
O UT ST A NDI NG F E AT U R E S
Colon ies grow ing rather restrictedl y , typi call y co ns isti ng 0 1' a close-tex turcd
ba sal fel t, w ith su rface growt h velve ty to almost lanat e , more a l' less co ns picuo us ly fu r rowed, heavily sporing th rough out, in variou s shades 0 1' dull yellowgree n, gray-gree n, ave lla neous to brown ; colony reverse usu all y in dull yellow ,
gree nish gray, 0 1' b row nish shades .
Conidiop ho res variable in length , com paratively long whe n borne di rectly
from th e su bstra tum, 0 1' short wh en a rising as branches fro m ae ria l hyphae,
usu all y smooth wa lled 0 1' del icat ely ro ughe ne d .
Marg inal stolo ns typi call y p roduced in m ost me mbe rs 01' th e series whe n
cultivated upon m oist su bs trata 0 1' under very humid cond itions.
Penicilli asym metrical , ty pica lly ra mi fied , characterized part icula rly by th e
co m pa ra tive sho rt and very co m pact b ase , with ra m i a p pressed , m et ul ae and
p hialides numerou s a nd closely cro wded; not ramified in sorne stra ins,
cons isti ng a l' terminal clusters a l' metul ae bearing crowded phi alides .
Conid ia globose , elliptica l lo more 01' less pyri form , smooth 0 1' delicately
ro ug he ned , wi th co nid ial chains tending to ad he re in mo re a l' less loosely
parallel colu m n s a l' definitely ta ng led .
K ey to species
Page
1. Penicilli typically rebranched below the level of metulae, with the main
axes and branches terminating in verticils of metulae. Penicilli comparatively short, with all elements closely appressed.
P. brevi-compactum series . . . . 394
A. Penicilli typically showing one or more side branches below the level
of metulae.
1. Conidia smooth-walled or nearly so.
P. vo/gaense Beljakova et Milko ... . 405
2. Conidia conspicuously roughened.
a. Conidiophore walls conspicuously roughened .
P. brevi-compactum Dierckx ... . 396
terminal verti cil 01' lil e! II lac supporting a cluste r 01' crowdcd phi alidcs. In SOIll"
stra ins thi s t yp e 01' pcni cillu s occu rs almos t cxclus ivcly. C on id ia l cha ins a re
variable in lcn gth , a ppearing loosely parallel 0 1' div er gcnt , with liule or no
tenden cy to form colu m ns , becoming tanglcd in ag c (lig . 142, C ).
Vi ewed under high magnificati on , th e peni cilli 0 1' mo st strains may be
stro ngly sugges tive 01' Penicillium citrinum a nd relat ed spec ies, exce pt 1'01' thcir
com pactness a nd larger size; viewed under low magnifi cati on, they show
masses 0 1' tan gled 01' div ergent chains 01' con id ia rather th an well-defin ed
colu m ns .
~L.
'i
(~~rl '1'\~{
~
'1
L...--..J
100 jirn
Penicillium brevi-compectum Dierckx - Ann. Soc oSci. Bruxelles. 25: 88. 1901. See
also Biourge's Monographie, La Cellule. 33: 155-157. 1923; also Ch. Thom, 'The
Penicillia', pp. 295-296. 1930; also Raper and Thorn, ' A Manual of the Penicillia',
pp. 407-411.1949.
Synonymies
Peniciltiurn cressum Sopp - Monographie, pp. 147-148. 1912. See also Ch.
Thom, 'The Penicillia', pp . 297-298.1930; sea also Raper and Thom , 'A Manual of
the Penicillia.' p. 410. 1949.
Penicillium blelowiezense Zaleski - Bull, Acad. Polonaise Sci. Math. Nat. Sr. B,
pp . 450-451. 1927. See also Ch. Thom, 'The Penicillia', pp. 303-304. 1930; also
Raper and Thom , 'A Manual of the Penicillia', pp. 410-411 .1949.
Penici//ium hagemi Zaleski - Bull , Acad . Polonaise Sci. Math. Nat. Sr. B, pp.
448-450. 1927. See also Ch. Thom , 'The Penicillia' , pp . 298-299. 1930; also Raper
and Thom , 'A Manual of the Penicillia', p. 411.1949.
Penicil/ium pa tris-meiZaleski - Bull, Acad. Polonaise Sci. Math. Nat. Sr. B, pp.
496-498. 1927. See also Ch. Thom, 'The Penicillia', p. 303. 1930; also Raper and
Thom, 'A Manual of the Penicillia', p. 411 . 1949.
Penici//ium szaferi Zaleski - Bull . Acad. Polonaise Sci. Math . Nat. Sr. B, pp .
447-448. 1927. See also Ch. Thom, 'The Penicillia', pp. 299-300. 1930; also Raper
and Thom , 'A Manua l of the Penicillia', p. 411 . 1949.
dclicat cly rou ghcn cd (Iig , 142 , B, aIHI SEM plnrc H, ce n tre: right); conid ial
chai ns becollling ta uglcd in age (Iig . 11-2 , C).
Colo n ics upon C YA (co lou r plat e 142 , B a nd b) all ainin g a diamc tcr 01' 15
m m in 14 d a ys a t roo m ternpcrature (20 - 25C), co ns ist ing 01' a close- tcx tu rcd
a nd tou gh basal felt , strongly radia tely urrowed a nd irregul arly rai sed , wi th
ab ru pt m a rgin sligh tly su b me rged, ex te nd ing less than 1 mm beyond th e aerial
growth; spori ng less ab u ndan tly than a boye in th e sa me sh ades; odou r lacking;
exudate lim ited 01' lacking; ye llow ish coloured pi gment diffusing in to th c
su rrou nd ing agar; colony reverse (colour piate 142 , b ) in reddish cin namo n
shades, strongly pulling th e agar a way fro rn th e cu lture dish a n d cracking it ;
penicilli esse ntia lly as describ ed aboye.
Colon ies upon M EA (co lour plat e 142 , C a n d c) a ttain ing a di amet er 01'
about 20 m m in 14 days at room temperature (20 -2 5C), con sisting 01' a
velvety felt, not furrowed, pla ne, with m a rgin la r gely su b merged, exte n di ng
abo u t 4 m m b eyon d th e aerial growth; ve ry slight ly rai sed in colony ce n tre ;
hea vily spo ring in the sa me shades as aboye; m argin m ore 0 1' less irregul arly
crene llate in old cu ltu res from the irregul a r develop me n t al' stolo nlike hyphae;
odo u r a n d ex udate lack in g ; no coloured pi gment diffu sing into the
su rrou nd ing agar; colony reve rse (co lour pia te 142 , c) in pale ye llow ish shades;
peni cilli esse n tially as described a boye but with the co n id iopho re walls more
definitely ro ughened (fig. 142, A).
T he species d escri ption is centere d upon strain CBS 2 10.28 = CMI92,266 =
IJFM 72 15, isolated by K. Zaleski in 1926 as the typ e strai n 01' h is Penicillium
patris-m ei, from soil under con ifers in Poland , a nd upon m any othe r strains
isolated b y DI' A.T. M a rtnez from a n andosol in th e Provin ce 01' N a varra ,
Sp ain , under Fagus silvatica L. (Colour pl a tes 24 1 a nd 242 presen t two
re p resen tati ve strains 01' P. brevi-compactum, isola ted fro m soil by A .T.
M art nez , 1977 .)
Penicillium brevi-compactum D erckx val'. magn um val' n o v . : fig. 223 (see
p . 616), colo u r plate 22 3, a n d SEM pl ate 24, (bottom : left).
Latin diagnosis of Penicillium brevi-compactum Dierckx varo magnum varo nov.
Coloniae in agaro Czapekii, post duas hebdomadas ad 20-25C, 35 mm in diametro attingunt, velutinae, laxo texto basali coacto mycelio, planae, cum margine
inaequaliter plerumque e hyphis (2 mm) submersis formatur, planae ad marginem,
umbonatae ad centrum; fortiter sporulantes, cum fructificationibus conidialibus
coeruleo-viridibus; exsudatio parco; odor abest; sine pigmento in medio diffluente;
reverso sine colore. Penicillus varietatis a Penicillium brevi-compactum Dierckx
differt in latitudine conidiophorum, ramorum et metularum; conidiophora laevia,
tenus 7.5-8.0 p,m in diametro; rami laevia, tenus 7 p,m in diametro; metulae laevia,
tenus 6.5 p,m in diametro; conidia globosa vel subglobosa, 3-4 p,m in diametro,
asperula , in intricatis catenis formantur.
Colonies upon Czapek's agar (eolour plate 223 , A and a) attaining a di ameter of 35 mm in 14 days at room temperatu re (20 -25 C), eonsisting of a
loose- tcxt u red basal felt , d eep ly velvety to su bla na te , not fur rowed , with thin
white margin su rrou nd ed by a fri nge of submerged hyph ae extending a bou t 2
111 111 beyond the ae ria l growth, wi th eolony su rface pla ne in marg inal and su bIlIargin al a reas, a nd abruptIy raised as a plateau in th e cen tre, overla id by a
loosely wov en ne twork of white floccose hyph ae len ding a lanate a p peara nce to
I he central a rea; heavily sporing th rou gh ou t in deep blue-g recn shades (Sg uy ,
1'1. XXXI , 46 1); wit h the blue predom inant ; exudate lim ited in a mo u nt,
rnmeshed in th e mycelial felt ; odour lacki ng ; no coloured pigme nt diffus ing
1I10 th e surrou nding ag a r ; eolony rev ers e (co lou r plat e 223 , a) eolourless;
I)('nicilli (fig . 223 , A) typ ically of the Penicill ium breoi-compactum type bu t
ons pic uously larger and sto uter, persistentI y smooth wa lIed, wit h conid iophores ereet , arising from the m ycelial basal felt a t the agar surface in a close
and even stand , usualI y straight a nd rigid, un scp ta tc , rangin g fro m 400 to 500
t m or more in lcngth by 7.5 - 8 .0 ;,m in diamcter, with a pices eom monly
swollcn u p to 10 ;,m in d iamete r; pen icilli usu all y consisti ng of cro wde d
vcrticils of closely a ppressed sto ut ram i, mostIy 10-1 8 tui: b y 5 -7 u u,
numbe ring u p to 10 elements or m ore in thc vc rticil, vert icils bearin g stou t
mctulae, com m only wedge shaped , measu ring 4 .5- 7.0 ;,m in dia m eter in a
med ian plane b y 9 -12 ;,m in length , enlarged a t the ap cx up to 7.5 ;,m in
d ia me ter; phialides bottIe shaped, mostIy in who rls of 3 -6 elc m ents in the
vcrtieil, us ually 9 - 14 ;,m by 4 .5 -5 .5 ;,m ; co nidia globose to subglobose,
3. 0 - 4 .0 ;,m in d ia me te r , wit h wa lls delicately ro ughened (fig . 223 , 8 , a nd
SEM plat e 24 , bottom : left); con id ial cha ins usu all y ta ng led (fig. 223, C) .
Colo nies u po n CYA sp read ing broadl y (colour plate 223 , B) , attai n ing a
d ia meter of 55 mm in 14 d ays at roo m tem peraturc (20- 25 C), consisting of a
deeply velvety felt, co nsp icuo us ly za nate a nd radia tely wrin kled, slightIy
ra iscd, with white thin m a rgi n surrou ndc d by a rin gc 01' su b me rged hyph ae
exte nd ing a bo u t 2 mm bcyond th e ac rial gro w th; co lo ny surf ace ovc rla id by a
loosely woven n etwork of'white Iloccose ae r ial hyph ae lendin g a co ttony a ppc arance to th e co lony; heavil y sporing throu gh out in th e sa me sh ades as aboy e;
ex u date limited in a mo u n t, as minute co lou rlc ss drops; odour lacking ; pale
a m be r colou red pi gment diffu sing in to th e su rrou n d ing agar ; colo ny reverso
(colour pIa te 223, b ) in pale orange sh ades; penicilli esse n tial1y as de scr ibcd
aboye.
C olonies upon MEA (co lour pIate 223, C a n d e) a tta in ing a di ameter 01' 30
mm in 14 d ays a t room temperature (20-25C), co ns isting of a deepl y velvety
elt, appear ing granu lar a nd co ns picuous ly zo nate, with irregul arly di ssectcd
m argin developing sto lon- Iike su bmerge d hyphae exte nd ing a bo u t 2 mm
beyond th e aeria l growth; hea vily spo ring throu gh out in th e sa me shades 01'
blue-green as a boye; colony su rface not fu r rowed a nd Ilat ; ex u date a nd od ou r
lacking; no coloured pigment diffusin g into the su rrou n d ing agar; colony
rever se (colou r pla te 223, c) in pale oran ge shades; pen icil1i esse ntia lly as
descri bed aboye .
The va riety description is ce n te re d upon th e typ e strain IJFM 59 54, isolated
by th e au thor as a cu ltu re co n ta m inan t, M adrid , Spain, May , 197 9 . It is
kn own only frorn th e typ e strain.
Penicillium breoi-compactum Di erckx va r. magnum d iffers m arkedl y fro rn the
ty pe species, particul arl y by its b ro adl y sp re ad ing colo n ies upon al1 subs tra ta
a nd by its sto u te r a n d larger penicil1i, but othe rw ise, it ap p rox imates th e ty pc
spec ies we l1 enou gh in th e characte r istics a ttr ib u te d b y R ap er a n d Thom to th e
spec ies to warrant recogn ition as a va riety a n d not as a sepa rate species in th e
P. breoi-compactum ser ies . Therefore , it is d escribed a nd p rop osed as a n ew
variety wi th th e spec ific n ame of Penicillium breoi-comp actum D ierckx va r.
magnu m . T he va r ieta l ep ithe t refers to th e broadl y spread ing colo n ies,
particularl y upon G YA (co lour plat e 223, B) a n d th e sto u t penicil1i (fig . 223,
A).
143 . Penicillium stoloniferum T ho m: fig. 143 , colour plate 143 , a nd SEM
plat e 24 , (b ottom : ri ght).
Penicil/ium stoloniferum Thom - Bull, Bur. Anim. Ind. U.S. Dep. Agric. 118: 68-69.
1910. Also Ch. Thom, 'The Penicillia', pp. 292-294. 1930; also Raper and Thom,
'A Manual of the Penicillia.' pp. 412-414. 1949.
Synonymies
Penicillium biourgeianum Zaleski - Bul!. Acad. Polonaise Sci. Math. Nat . Sr. B,
pp. 462-464. 1927. See also Ch. Thom, 'The Penicillia', pp. 296-297. 1930; also
Raper and Thorn, 'A Manual of the Penicillia' , p. 414. 1949.
0 0
0 80 O
O
ca 00 0 0
O 0 00 O
O 00
00
O
00
,~~
1~~~'\
~r~'
0:,'4
1~1 ~
100
jirn
fR .
Penicillium erectum Bainier - Bull. Soc o Mycol. France. 23: 13. 1907. See also
Ch. Tho m, 'The Penicillia' , p. 295. 1930; also Raper and Thom , 'A Manual of the
Penicillia', p. 413. 1949.
Penicillium griseo-brunneum Dierckx - Ann . Soc o Sci. Bruxelles. 25: 88. 1901.
See also Biourge's.Monographie, La Cellule. 33: 162-163. 1923; also Ch. Thom ,
'The Penicillia', p. 302. 1930; also Raper and Thom, 'A Manual of the Penicillia' ,
p. 413. 1949.
Penicillium tabascens Westling - Arkiv . Bol. 11 : 56, 100-102. 1911. See also Ch.
Thorn, 'The Penicillia' , pp . 300-301. 1930; also Raper and Thom , 'A Manual of the
Penicillia', pp . 413-414. 1949.
XXIX, 12G), hu! lI\oslly ob scu red hy th c lloccos ovcrgrowth 01' whit e acrinl
hyphac; thin and Ila! m argin su r rou ndc d by a narrow fringe 01' submcrgcd
hyph ac ext ending less than 1 mm beyond th e ac ria l growth ; cxudate ami
odou r lackin g ; pale yellow pigment slowly diffu sing into the surrounding agar;
colony rev erse (col our pi ate 143, a) in pale cre a m sh ad es; penicilli (lig. 143, A)
typically co m p ac t with all elements clos ely a p p re ssed, forming loosely p arall cl
or divergent ch ains 01' con id ia , presenting th e pattern 01' short , co m pact
brushes, with con id iopho res a r ising eithe r from submerged hyphae at th e agar
su rface, or a s lateral ramifications from aerial h yphae , the forrn er rangin g from
100 to 350 flm in length by 3. 0 - 3 .5 flm in di ameter, the latter from 35 lO 100
flm by 3 .0 - 3 .5 tuts , smooth wall ed; larger penicilli co ns isting usu all y a l'
ve rticils 01' metulae a nd sometimes on e , or occasion ally more , sid e rami ea ch
terminated in a whorl a l' m etulae; smalle r penicilli com mo nly co nsisting 01' a
sin gle terminal ve rticil 01' closely a pp res sed m etulae each bearing cro wde d
phialidcs ; rami , when present usuall y 10-25 flm by 3. 0 -3 .5 flm ; m etulac ,
ran ging from 3 to 8 in number in th e verticil, mostly 11-1 5 iut: b y 2 .8-5 .0 flm ,
with a p ices e nla rged up to 4- 6 flm in di amet er; phialides in co m pac t cluste rs
4- 8 elemen ts in th e verticil , b ottl e sh aped , mostly 7-11 flm b y 3- 4 flm,
taper ing to a sho rt n arrowed neck ; con id ia elliptica l wh en you ng , but
becoming globose to subglobose a t m aturity , mostly 2.5-3 .5 flm in di amet er,
appear ing smooth walled 0 1' nearly so when obs erve d under th e light
mi cr oscope (fig. 143 , B) but fin ely roughened when obse rve d under th e SEM
(pl at e 24 , bottom : right); co n id ial cha ins forming loosel y parallcl or divergent
colu m ns (fig. 143, C) .
Colon ies upon CYA (col our pl at e 143 , B and b) sp re ad ing broadly , a tta in ing
a di ameter a l' 50 mm in 14 days at room temperature (20- 25 C ), co ns isting 01'
a close -textu re d ba sal felt , deepl y velve ty a nd stron gly sulcated by numerous
radial furrows, grad ually raised tow ard s th e colony ce n tre, with e n tire m argin
white ; heavily spor ing th roughout in blue- gr een shades (Sguy, PI. XXX,
443) ; conspicuously zonate; odo ur faint, indefinite ; ex u date abundantly
produced , as crys tal clear , p al e a m bc r-colou rc d drops ; a m be r-colou red
pi gment d iffu sing into th e surro u nd ing aga r ; colony reverse (co lour plat e 143,
b) in sa ndy to cinnamon shades, a nd strongly pulling a n d splitting th e
underlayin g agar ; penicilli esse n tially as described a boye .
C olon ies upon MEA (colour plate 143 , C a nd c) growing restrictcdl y,
att aining a di amet er 01' ab ou t 25 mm in 14 d ays a t ro om temperature
(20- 25C), con sisting 01' a dense velvety felt , slightly radiat ely furro wed in th e
ce ntre, alm os t flat , with irregularly di ssected ye llow m a rgin largel y su b me rged
ex te nd ing a bo u t 4 mm beyond th e ae
r ia l growth, co ns isting 01' stolon-like
/
hyphae co m para tively stout ; h ea vily spo ring throu ghout in gray-g reen shad es
(S guy, PI. XXIX , 428); exu date a n d odo u r lacking ; no coloured pi gment
diffusing into the surrounding ag a r; colony rev erse (co lou r piat e 143, e) in
I-H . Penicillium paxilli Bainier: fig. 144, ca la u r plat e 144 , and SEM plate
::!:i, (tap : left) .
W-!J..LJ.J..W
10J,lm
O O 00
B O
OQO
DO O
'i".""
:';;'~ t
. _,~.
-","
.,. ~ \~\,
'/I/~
"1"
100J,lm
fR.
Penicillium paxilli Bainier - Bul. Soc oMycol. France. 23: 95-96. 1907. See also Ch.
Thom, 'The Penicillia' , pp. 294-296. 1930; also Raper and Thorn, 'A Manual of the
Penicillia.' pp. 414-416. 1949.
co ns p icuo us ly zo na tc, almos t planc 01' show in g lew sha llow ra d ial u rrow s:
colo ny su rlacc ovc rla id by a loosely WOVCII nctwork 01' whit e loccosc ac ria l
h yphae , either in localized sec to rs 0 1' in marginal arcas, rorn whi ch scco ndury
conidial structu res are dcvelop ed ; flat grow ing m argin white su rro u nclcd by /1
narrow frin ge 01' sub me rge d hyphae exte nd ing about 1 mm beyond th e aeriul
grow th; heavily spo r ing throu gh ou t , at first in avella neo us sha des (Sguy , 1'1.
XIII , 190) during th e first 2 week s, sha d ing th e blue- gr een (Sguy , PI. XXI X,
425) with th e late ripe ning 01' con id ia; exudate a nd od our lacking; pal e a mb crcoloured pi gm ent slowly di ffusing into th e surrou nd ing agar; colony reverso in
dull yellow to cinnam on sha des (colour plat e 144 , a); peni cilli typically
com pact (fig. 144 , A), consisting usually 01' a sing le termi nal ve rticil 01' 2- 11
metulae from which arise di vergent chains and loos e colu m n s 01' conidia (Ii g.
144 , C) ; con id iopho re s arising primarily from th e substratum in a den se anrl
eve n sta nd, occasionally a s short branches from aeri al hyphae , va riable in
len gth , com mo nly 200 -300 p.m by 2. 5 - 4 .0 p.m , so me times longer u p lo GOO
tuu ; with walls sm ooth 01' nearl y so; metulae 12-1 5 p.m by 3 .0- 4 .5 uit, wi th
apices enla rged to a vesicle up to 4- 6 p.m in d iamet er; phialides bottle sha pcd ,
in wh orl s 01' 3-9 eleme nts in th e verticil , mos tly 8-11 p.m by 3 .0-3. 5 .ull ,
tap ering abr u ptly to a sho rt narrowed neck ; conid ia mostl y ellipticnl,
com mo nly 3 .0 - 3 .5 p.m by 2.0 -3 .0 p.m in d iame te r, with walls smoo th (J I'
neari y so wh en obse rve d under th e light mi croscop e (fig. 144, B) but delical ely
rou ghened wh en obse rved under th e SEM (pIate 25, top : left ) ; con id ial chains
loosely parallel 0 1' divergent colu m ns (fig . 144 , C).
Colonies upon C YA (colour plat e 144 , B a nd b) spreadi ng broadly , atta ining
a di ameter 01' 50 mm in 14 days at roo m temperature (20-25 C), cons isting 01'
a sim ila riy textured ba sal felt as aboye, but m arked by numerous and dcep
radial fu rro ws, with ce ntre some wha t raised and irregul ari y fold ed , wi th a thin
white margin surro u nde d by a n arrow frin ge 01' su b me rge d hyphae extend ing
abo ut 1 mm beyond th e ae rial gro wth; heavily spo ring th rough out at first in
ave lla neo us shades (Sguy, PI. XIII , 190), th en becoming blue-gr een colou red
with the rip en in g 01' con id ia (Sguy, PI. XXXI, 463 ) whi ch sta rts in margin al
areas; odo u r lacking 0 1' faint ; ex udate lacking ; pale amber-pigment diffusin g
into th e su rround ing aga r; colony reverse (colou r plat e 144 , b) in orange to
cinnamo n shades ; peni cilli esse nt ially as descr ibed aboye .
Colonies upon M EA (colour plate 144 , C and c) spreadi ng broadl y,
atta in ing a di ameter 01' 50 mm in 14 day s at room temperature (20 - 25C) ,
consisting 01' a looser textured ba sal felt , conspicuously za nate , typi cally
velvety , with ve ry thin m argin su rrou nde d by a eringe 01' su bme rged hyphae
exte nd ing abo ut 2 m m beyond th e ae rial gro wth; colony su rface plane , not
furro wed ; heavily spo ring througho u t in brownish gree n shades (Sguy, PI.
XXI , 303) an d rem aining persistently so ; ex udate and odo ur lacking; no
coloured pigment di ffusin g in to th e su rrou nd ing agar; colon y reverse (colour
plute 144 , e) dcvclopin g ycllowish cinnamon shudcs with a grec nish cast
showing throu gh ; peni cilli essc n tia lly as descri bcd aboye .
'I'he species descri ption is cen tercd u pon thc type stra in c ss 360 .48 = C M I
'10 ,226 = AT C C 10,480 = NRR L 2,008 = QM 725 = IJ FM 7122 , isolat ed by Prof.
W. C. Hutchinson , Bar ro Colora do Island, Panam a , from optical in struments,
I !l17. Stra ins of thi s species are frequently isolat ed from deteri or ating mili ta ry
cq uipment , from fleshy fung i, nuts, etc .
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hyph ae, heavlly sporlng, at first blue-gray wlth a greenlsh cast , th en (after 45 days)
shading to olive gray to tobacco, and usually (after 3 months) becoming violet-gray
in colour; with even white margin, 1-1.5 mm wide; exudate Iimited, colourless;
colony reverse in pale lemon yellow shades. Conidiophores erect, not branched,
occasionally with a side branch 260-400 fLm by 4-4.6 fLm, with apices inflated up to
5 (7.S) fLm in diameter, delicately roughened, arising either from the substratum or
as branches from aerial hyphae or ropes of hyphae (particularly in the colony
centre). Penicilli typically cornpact, usually symmetrical, two- to three-stage
branched , and asymmetrical with a side branch under the level of the metulae .
Branches when present 9-11.7 fLm by 2.5-3.3 fLm, with apices enlarged in a vesicle
up to 3.6 fLm in diameter, usually numbering 4 in the verticil. Metulae 7.S-10.0 fLm
by 2.6-4.0 fLm, slightly enlarged at the apex, numbering up to 5 elements per
verticil. Phialides 7.S-9.0 fLm by 2.0 fLm, numbering 6-S per cluster. Conidia rough
walled, at first elliptical, 2.6-3.3 fLm by 2.0-2.6 fLm, then becoming globose,
2.6-3.6 fLm in diameter forming more or less divergent chains, blue-gray coloured in
mass.
Colonies upon malt extract agar appearing velvety to tanate, heavily sporing, at
first bluish gray, with margin white up to 2 mm wide, then becoming (after 45 days)
dark gray, with the colony reverse reddish coloured. Conidiophores arising either
from the substratum and from aerial hyphae or from ropes of hyphae . Otherwise as
described aboye. Odour faint, suggestive of dried fleshy fungi. Maximum
temperature of growth about 30C, minimum SOC, optimum 20-26C.'
(Translated from the Russian by Prof. L. Vilas).
O ur no tes follow:
Co lonies u pon CA (colour plat e 145, A and a) growing rather rest rictedly ,
attaining a d ia meter of 30 mm in 14 days at roo m tem perature (20-25 C) ,
consis ting of a close -tex tured b asal felt ove rla id by a dense network of white
floccose ae ria l hyphae len din g a deeply lanat e appearance to the colony
surface; slight ly raised in th e ce nt re , not fu rro wed, with a thin eve n m argin
surrou nde d by a narrow fringe of su bm er ged hyphae exte nd ing a bout 2 mm
beyo nd th e aerial grow th; medium sporing throughout in gray-gre en shade s
(Sguy, PI. XXVII , 404), but with conid ial areas almost com pletely obs cu red
by th e floccose ove rg row th of white ae rial hyphae ; exudate and odou r lacking;
pale yellow -coloured pigment di ffusin g into th e su rround ing agar; colony
reverse (co lour pIate 145, a) in pale yellow shade s; peni cilli (fig . 145, A) as
descr ibed aboye, suggestivc of th e Penicillium paxilli typ e , usu all y consisting of
small ve rticils of ram i, ranging from 20 to 25 I-tm in length by 2.0 -2.5 I-tm in
di am eter, be a ring wh orl s of 2- 3 metulae , mostly 9- 15 I-tm by 2 .5 -5 .0 tut,
ea ch su pp orting cro wded clus ters of 2- 8 phial ides u suall y 8 -1 OI-tm by 2.5 - 3 .8
tut , tape r ing to a short narrowed neck ; all ele me nts in th e penicillu s are m or e
or less closely appressed ; co nid iopho res smooth walled , com mo nly short , up to
I!jO Itlll in lcn grh hy 2 .:i -:~ . 0 Itlll in di nmctc r , ari sing' cithe r rom the
1I11i )slra tlllll 01' as short branch es Irom ae ria l hyph ac 01' from rop es 01' hyph ac ,
pur ticulurly in th e colony ce nt re; co nid ia cllipiical :1 .0 - 4 .5 utt: by 2.2-2. 8 tm
11) subg lobose , a ppear ing smoo th walled or nearly so wh en obse rve d under th e
ligll( micro scop e (Ii g. 145, B) but deli cately rou gh ened wh en obse rve d under
t lu: SE M (plate 25, top : ri ght) ; conid ial chains forming loosely paraUeI 01'
dcflnir ely ta ng led (fig . 145, C ).
Colonies upon C YA (colour pla te 145 , B a nd b), a ttain ing a diameter 01' 65
111111 in 14 days at room temperature (20- 25C), cons isting 01' a c1ose-textured
hasa l clt , a ppearing deepl y velvety to subla nate 0 1' funi cul ose , m arked by
1III III CroUS and deep radi al fur row s, slightly ra ised in the ce ntre, with thin white
Illa rgin surrou nded by a wide frin ge 01' su bmerge d hyphae exte nd ing about 4
111111 bcyond the ae rial growth ; heavily sporing throughout in brownish gray
sltadcs (Sguy, PI. X LVII , 694), with conid ial a reas loosely ove rla id by a
ru-twork 01' floccose and fu n iculose veget ative hyphae ; odo ur and exudate
la('kin g; pale yeUow-colou red pigment d iffusing in to the surrou nd ing agar;
ro lony reverse in cin namon shades (colour plat e 145, b); peni ciUi esse ntia Uy as
. k-sc rib ed abo ye.
C olonies u pon M EA (colour pia te 145, C and c) growing m or e res trictedly
Ihan up on C YA , atta ining a di amet er 01' 45 mm in 14 days at roo m
11'llIperatu re (20-25 C), co ns isting 01' a den se velvety felt , com pa ratively thin
.uid flat , not furrowed, with th in eve n m argin mostly su bme rge d , exte ndi ng
nbout 4 mm beyond the ae rial growth, colony su rface loosely ove rla id by a
uct work 01' ae rial ve geta tive hyph ae and rop es 01' h yphae ; heavily spo ring
throug ho ut in a rtemisia gree n shades (Sg uy, PI. XXIX , 429); exudate a nd
odo ur lacking ; no coloured pigment di ffusin g into th e surrou nd ing agar;
ro lony reverse (colour plat e 145 , c) developing yeUowish cin namo n sha des ;
pcni cilli esse nt ially as descri bed aboye.
T he species description is ce ntered upon the typ e strain CBS 626. 72 = VKM
11-1 ,127 = C M I 167 ,384 = IJ F M 7114 , isolat ed by L.A . Belj ak ov a and A .A .
Milko in 1966 fro m soil near Pless, U SSR. It is kn own only from th e type
st ra in .
Penicillium volgaense appears to be most sa tisfac torily ass ignable to th e P. breoirompactum series near P. paxilli Bainier on accou nt 01' the resemblance 01' its
peni ciUi with those 01' th e la tter spec ies. The presen ce 01' terminal ve rticils 01'
metulae in both form s is stro ng ly su ggestive 01' th e Penicillium citrinum series,
bu t they d iffer from it by its larger peni ciUi with aU elements m or e close ly
app resse d .
146 . Penicillium skrj abinii Sh moti na el Golov leva : fi g. 146 , colour plate
146 , a nd SE M plat e 25, (centre : Ieft ).
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Shmotina and Golovleva 's original diagnosis follows, as this adequately presents
the species in question when recently isolated :
' Colonies upon Czapek's agar attaining a diameter of 38-46 mm in 12-14 days,
velvety, radiately furrowed, surface irregular, w ith centre folded, pale pink
coloured , w ith margin granular surrounded by a fringe of submerged hyphae
extending 1-2 mm beyond the aerial growth, somewhat zonate. in blue-green
shades , green , dull green , or chrysolite green colours; colony reverse at first cream
coloured, then shading to terracotta in age, with marginal and submarginal areas in
lemon yellow , sulphur yellow to whitish tints. Exudate colourless or cream
coloured , limited in amount or lacking . Odour pleasant (suggestive of the cheese
mould's aroma). Conid iophores erect, not ramified, with apices enlarged up to 4-5
Ilm in diameter, conspicuously warty, arising from the substratum or as branches
from aerial hyphae ranging from 25 to 500 Ilm, or up to 600 Ilm in length by 2.1 Ilm in
diameter. Penicilli cornpact, mostly biverticillate and asymmetric, occasionally
Imple lappearing monoverti eillate) ; bran ehes wh on present , in number of one per
punlcillus, elosely appressed against th e main axis or divergent, eonspieuously
w nrty, 26.4-32 .tm or up to 40 Ilm in length by 2.4-3 .tm up to 3.2 .tm in diameter;
rnot ulae parallel or somewhat divergent, with apiees inflated up to 4 .tm in diameter,
onspieuously warty, (3-4-6-8) 7.7 .tm- 16.1 .tm up to 20.3 .tm in length by
.1- 2.8, up to 3.0 .tm in diameter; phialides in erowded vert ieils, 6-8 elements in
lile whorl, bottle shaped, tapering to a narrow neek up to 2 .tm long , about 7.7 -10.5
IHl1 up to 19 .tm by 2.3-3.0 .tm, up to 3.5 Jlm; eonidia elliptieal, globose or
subqlobose, rough walled, 1.8-2.4 Jlm by 3.5-5.0 .tm in diameter, in ehains of
50- 100 Jlm long .
Colonies upon malt extraet agar attaining a diameter of 47-54 mm in 10 days,
wa nular, w ith centre velvety, slightly raised, irregularly folded in a radial pattern, in
loccose seetors narrowing towards the eolon y margino The eolony surfaee
coloured in dark green to lead gray shades, with seetors in lead gray t ints and
colony cent re and margin white ; colony reverse developing tobaceo shades,
radiat ely furrowed in the centre . Exudate Iimited in amount, as minute droplets
enmeshed in the mycelial felt. Odour pleasant , 'mouldv' .'
(Translated from the Russian by Prof. L. Vilas ).
SEM plat c 25, centre : lc ft); co nid iul chnins lormin g loosely para lid lo
divcrgent (Iig. 146, C ).
Colon ies upon C YA (colour plate 146 , 13 and b), aua in ing a di am ct cr of 40
mm in 14 days at room temperature (20-25 C), co n sistin g o!" a closc- rextu red
basal felt, d eep ly velv ety , somewhat zanate, strongly and dccply radiatc ly
furrowed, with centre irregu la rly fold ed and sligh tly rai sed, with colon y surfacc
overlaid by a thin , closely wov en n etwork of fine white aerial hyp hae influcn cin g gr eatly the colony appearance; margin entire su rro u nde d by a very
narrow fringe of submerged hyphae extending less than 4 mm beyond th e
aerial growth; sporing we ll but gr adually , starting at th e margin and thcn
proceeding towards th e centre in deep blue-green shades (Sguy, PI. XXXI,
461), with the ripening of conidia, elsewhere developing cin n a m on shades;
odour faint, ' m ouldy'; exudate abundantl y produced , as pale amber-coloured
drops ; a m be r-coloured pi gment diffusing into th e surrounding agar; colony
rev erse (colour plate 146 , b) developing reddish brown shades; penicilli essentiall y as des cribed ab oye .
Colonies upon MEA (co lour pl at e 146, C a n d c) attainin g a diamet er of 40
mm in 14 days a t room temperature (20 -25 C) , consisti ng of a loos e-textured
basal felt , deeply velv ety to sublanate, somewhat tufted by means of whitc,
floccos e, interlacing, ae r ial hyphae, with a very th in m argin surrounded by a
fringe of submerged hyphae exte n d in g ab ou t 3 mm beyond th e aeri al growth;
he av ily sporing throughout in deep gray-green shades (Sguy , PI. XXI, 308,
309); odour faint , aromatic; ex u date lackin g ; no coloured pigment diffusing
into th e surrounding agar; colon y rev erse (co lour plate 146 , c) dev elop in g
yellowish oran ge sh ad es with a green ca st showin g through ; penicilli as
described aboye but with longer stipes a rising primarily from the substratum.
The spe cies description is ce n te re d upon th e typ e strain CBS 439 .75 = VKM
F-1940 = IJFM 7123 , isolated in 1971 by L.A . Golovleva from soil , near the
village of Mujink, in the vicinity of Blagoveshczensk, in th e Amur region,
USSR.
Penicillium skrjabinii is a distincti ve sp ecies which appears mo st clo sely related
to the spe cies included within the P. brevi-compactum seri es , particu larly to P.
paxilli Bainier and P. stoloniferum Thom, but it can easily be differentiated from
both of th em by its conspicuous ly warty stipes and by its cu ltu ral ch a racteristics .
147 . Penicillium arenicola ChaIab uda: fig. 147, coIour pIate 14 7, and
SEM pIate 25, (centre: right) .
Penicil!ium arenicola Chalabuda - Nov. Syst . Crypt . Inst. Bot. Komarov ii, Acad.
Sci. USSR . 6: 162. 1950. See also M.M. Kulik, 'A Compilaton of Descriptions of
New Penicil!ium Species'. Agric. Handbook No. 351. Agre. Res. Ser. U.S. Dep.
Agr ie. p. 16. 1968.
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syno ny rnized with P. ochraceum b y Smith (196 3), but followin g the opinion of
Sa mso n et al. (1976), at present P. brunneo-stoloniferum ca n best be regarded as a
separa te spec ies. In orde r to va lidate the spec ies a L atin di a gn osi s of it is given
below; th e n ame of the ori gin al a u thor is m aintained under R ecom m end ation
46 C of th e International Code of Bot anica l N omenclature (Stafleu (E d .),
1972 .).
Latin dia gn osis of Penicillium brunn eo-stoloniferum Ab e ex R amrez :
Coloniae in agaro Czapekii, post duas hebdomadas ad 20-25C, 45 mm in
diametro attingunt, velutinae, planae , denso te xto basali coacto mycelio , parum
prominentes ad centrum , cum aequabili margine e submersis hyphis (3 mm)
formatur; fortiter sporulantes fusco colorantur; exsudatio adest , in magnis guttulis
fl avis; odo r abest; reverso sine colore vel pallido flavo; cum pigmento flavo
diffluente in medio; penici lli pleraque biverticillati asymmetrici, valde compacti les,
brev is; con idiophora septata, laevia , e substrato vel e mycelio basali exoriuntur,
plerumque usque 120-200 .tm in longitudine per 4.0-5.0 .tm in diametro, cum
apicibus tenus 4.5-5.5 Jt m inflatibus; rami brevia, 17-20 Jtm per 3.5-4.0 Jtm;
metulae 3-6 per verticillum, plerumque 20-30 Jtm per 5.0-5.5 Jtm; phialides
lagaeniformae, 13-24 Jtm per 4.0-5.0 Jtm, 4-7 per verticillum, cum exiguo
acuminato collulo; conidia elliptica vel subglobosa, plerumque 4-6 Jtm per 3.5-4.0
Jtm in diametro, fortiter echinulata; in catenis laxis parallelis vel diversis formantur.
Neque ascomata neque sclerotia visa sunt.
Coloniae in agaro Czapekii cum extracto levedinis, post duas hebdomadas ad
20-25C, 40 mm in diametero attingunt, velutinae, cum parum radiatis sulcis,
denso texto basali coacto mycelio, cum margine continuo; fortiter sporulantes,
fusco colorantur; exsudatio adest, magnis succineis guttulis; odor abest; cum
pigmento succineo in medio diffluente; reverso rubro cinnamomeo. Neque
ascomata neque sclerotia visa sunt.
Coloniae in agaro hordeaceo, post duas hebdomadas ad 20-25C , 30 mm in
diametro attingunt; cum margine inaequaliter, velutinae, planae; fortiter
sporulantes, fusco colorantur; odor et exsudatio absunt; sine pigmento diffluente in
medio; reverso ochraceo cinnamomeo; neque ascomata neque sclerotia visa sunt.
Typus cultura e solo Japonico a S. Abe et Y . Nakano in anno 1949 isolata est,
FAT 1132 = CBS 317.59.
lescribcd above but dccper velv cty und with Iew deep radial urrow s, with
e vc n mur gin c n tirc ; heavily spo r ing throu ghout in brown shadcs (S guy, PI.
X LV 11, 696 ); cxud a te abundantly produced, as yellow-coloured drops ; odo u r
luckin g; amber-colourcd pigment difu sin g into th e su r ro u nd ing a gar; colony
rc vc rsc dev eloping reddish ochrc sh ades (colour plate 148 , b) ; penicil1i
l'ssentially as described aboye .
C olon ies upon MEA (co lou r plat e 148, C a nd c) grow ing restrict edl y ,
uu ainin g a di amct er 01' a bo u t 20 mm in 14 days a t room tempera ture
t ~ () - 25 C) , co ns isting 01' a d en se velvet y felt , almost flat , not fur rawed , with a
slight clev ation in th e colony ce n tre in a cra te r ifor m pattern , with irregul arly
.Iissccted m argin ; hea vily spo ring throu gh out in dark brown shades (Sgu y , PI.
X LV II, 701) ; ex u date a nd odo u r lacking; no coloured pi gment diffu sin g into
lile su r ro u nd ing agar; colony reverse (co lou r plate 148, c) dev eloping ochre
shades ; penicil1i esse ntially as descr ibed a bo ye.
Thc sp ecies d escription is cen te re d upon th e typ e strain C BS 3 17.59 = C M I
hH ,217 = ATCC 18 ,311 = IFO 5 ,72 7 = FAT 1,132 = QM 7, 306 = IJFM 7136 ,
.solat ed in 194 9 by S. Abe a n d Y . N a ka no frorn Japanese soil.
Penicillium brunneo-stoloniferum Abe is a di stinctive spec ies whi ch a ppears to be
III ClSt sa tisfac to r ily ass ignable to th e P. breoi-compactum se ries th an a ny whe re
lsc , but it clearly differs fro m th e othe r species included in it , b oth in its
ultu ral characte r istic s a nd in th e d etails 01' m orphology .
Colonle s grown on agar have a velvety textura and are white. The surface of the
colony is smooth and the underside is cherry yellow. The colony exudes a cherry
yellow substance int o the culture medium during cultivation.
It is difficult to induce conidia formation by P. amagasakiense on the above three
media . To observe the production of conidia , potato agar was used. The penicillium
was then typically biverticillate and asymmetric. Conidiophores of 80-200 ,tm by
3-4.5 ,tm arising from the substratum. No branches were observed. Metulae usually
in groups of 4-8, 9-15 ,tm by 2.5-4.0 ,tm, with the apices not enlarged.
To classify this strain, some microbial properties of P. amagasakiense were
observed. The results indicated that the growth of this strain is very rapid on Czapek
or steep agars and that it can assimilate nitrite . The above properties, the textura
and colour of the surface and the reverse of the colonies, and the exudate were
fairly similar to those of P. !i!acinum Thom . There are, however, sorne differences
between .the two Penicillia: Penici!lium amagasakiense rarely forrns conidia and P.
!i!acinum cannot produce glucose oxidase.' (Without a Latin diagnosis).
Penici//ium aspergi//oides Rudakov - J . Bot. U.S.S.R. 44: 867.1959. See also M.M.
Kulik, 'A Compilation of Descriptions of New Penici!lium Species'. Agric.
Handbook No. 351. Agric . Res. Ser. U.S. Dep. Agric. pp. 17-18. 1968.
Penicillium aspergilloides Rudak ov is a spec ies described in 1959 that is
believed to be closely relat ed to the species included in th e Asym metri ca - velutin a su b-sectio n. H owever, it is presented here as a no ther form of
dubiou s positi on and ha s not been included in th e keys to spe cies in th e present
study for th e same reasons not ed abo ye .
Rudak ov ' s original di agn osis follows :
'On solid media it forms circular colonies 2-3 mm deep. Colour of the mycelium
white. The growing margin is surrounded by a fringe in a radial pattern 1 mm long .
After 3-4 days a pale orange colour appears in the colony centre . This colour
gradually becoming darker and extending to all hyphae which grow wider upon the
substrate to 40-50 mm in diameter.
In aging the aerial hyphae are lost, substituted by a layer of conidial structures.
On further growth, colony colours become duller and duller, changing from orange
to dirty crimson . Sometimes short tufts of gray aerial hyphae appear in the colony
centre. Yellowish milky-coloured pigment diffusing into the surrounding agar.
Conidiophores of young colonies are rather short. When older, the conidiophores
are coarser and longer. In old cultures , the conid iophores become slightly curved,
sabre shaped, 1.5 mm long by 5-6 ,tm in diameter.
The colour of the conidiophores is pale brown with an orange casto The walls are
thick and smooth . The penicilli consist of verticils of metulae, each bearing a whorl
of phialides, or occasionally simple penicilli (appearing monoverticillate) consisting
of small c1usters of phialides at the apex of the conidiophore. Usually variously
rmn llud, w ith rami arranged in seve ral stagos bolow the level of the metulae, but
Inootly ono -sided branched . Rami are usu ally arranged together in the pattern of a
Illr oo-pronged penicillus, abou t 30 J!m in longth , bearing at their apices small
vurtlclle of generally 2 metulae per cluster, about 9 J!m in length by 3.8 p,m in
lmnutor, usually cylindrical.
Phlolides are bottle shaped somewhat swollen in the middle, tapering to a
narrowed neck, commonly 7.5 by 2.3 p,m, in small c1usters of 3 elements per verticil.
nidia ellipsoidal, colourless, with smooth walls , 1.9-2.4 J!m by 2.2-2 .8 J!m ,
fonnlng long cha ins , loosely parallel or divergent .' (Without a Latin diagnosis).
ITrenslated from the Russian by Prof. L. Vilas) .
'mic:illium pidoplichkanum Chalabuda - Nov. Syst . Ins . Bot. Komaravii, Acad. Sci .
U.S. S .R. 6: 161. 1950. See also M.M. Kulik , ' A Compilation of Descriptions of
Now Penicillium Species' . Ag ric. Handboo k No. 351. Agric. Res. Ser . U. S. Dep .
Agrc . pp. 65-66.1968.
l 'rnictllium pidop lichkan um Chalabuda is a no the r species of dubious position
11t ;1I is believed to be closcl y rel ated to th e spec ies included in th e A symme-
419
haptcr 6
Asyrnrnetrica-lanata sub-section
IN THI S SUB -SECT ION , T HE C O LON IES T YPICALL Y CONS IST OF COTTONY, LA NAT E OR
deepl y floccose networks of fin e aerial veget ati ve hyphae ove rlaying th e basal
k it, with co nidiopho res gen erally arising as branch es from ae rial h yphae , less
lrcquen tly from the su bs tra tu m , with co nid ial struc tures com mo nly a p pe aring
at first in th e colon y cent re, th en progr essin g gradually toward s th e m argin al
areas . Pen icilli u su ally com parat ively large, irregula rly ram ified, with metulae
a nd phi ali des frequently arising at different nod es of th e con id iophore . The
conid iopho res are usu all y fairl y coa rse with wall s more or less rou ghened.
Conidia l cha ins forming tangled m asses ra ther th an well-de fine d colu m ns .
T he lin es of demarcati on between the Lanata su b-sec tion a nd th e other su bsect ions of th e Asymmetrica are ad mi ttedly arb itra ry. T he L anata su b -sec tion,
indee d, me rges in sen sibl y into each of the ot he r su b-sec tions of the
Asymme trica, and species are pl aced in one or th e other on th e judgement of
the obse rve r.
Fo rms that m ay be strongly lanate but produce typi call y di varicat e pe n icilli
are placed in th e Divaricata. Sp ecies wh ich m ay sho w more or less floccul en t
colonies but a p pearing velvet y are included in th e vclu tina . Form s
predominan tly lanat e , but sho wing tra iling or a sce nd ing ropes of h yphae are
ass igne d to th e Funicul osa . Species with con id iop ho res a rising primarily fro m
the su bs tratu m, collec ting in d efinite bundles or tufts, are placed in the
Fascicul at a.
In fact , th e Lan ata su b-sec tio n of th e Asymmetrica is a n artificia l gro u ping
01' forms wh ich is ret ained here primarily as a m att er 01' conve nience , a nd is not
pres u me d, th erefore , to represent a natu ral grou ping of spec ies th at should be
cxpec ted to ex h ib it unique bioche m ical or ph ysiological charac te ristics.
O f th e nine spec ies recogni zcd by R aper a nd Thom ( 1949) in thi s series,
only Penicillium camemberti T ho m, P. comm une Thom , and P. lanosum W estling
still remain a s separate species, th e other six, namely P. caseicolum Bainier, P.
lanoso-viride Thom , P. lanosocoeruleum Thom , P. biforme Thom , P. lanoso-griseum
T ho m, and P. aurantio-candidum Di erckx h ave been reduced to sim ple
synony m ies: P. caseicolum Bainier is ac tually regarded as syno ny mo us with P.
camemberti Thom (Samson et al. 1976), P. lanoso-viride Thom is regarded as
syno ny mo us with P. verrucosum Di er ckx va ro verrucosum (Samson et al. 197 6),
-r "- \1
and th e rernaining four spe cics are a c tu ully rcgu rded as syno ny rnous with l ',
Pag
1. Colonies typically lanate, without evidence of synnemata or ropes of
hyphae or with such structures reduced and insconspicuous if present o
A. Colonies remaining persistently white with the ripening of conidia or
becoming slightly coloured in gray-green shades.
P. camemberti series . ... 420
1. AII elements of the conidiophore except the stipe smooth walled.
P. camembertiThom . . . . 421
2. Conidiophores conspicuously roughened.
P. griseum Bonorden ... . 423
B. Colonies developing some shade of green or brown.
P. commune series . ... 425
1. Conidiophore walls roughened .
a. Conidia smooth walled or nearly so.
P. commune Thom . ... 426
b. Conidia strongly echinulate.
P. giganteum Roy et Singh . .. . 433
2. Conidiophore walls smooth.
a. Conidia globose or nearly so, less than 4 Lm in diameter, delicately roughened.
P. /anosum Westling .. .. 428
b. Conidia strongly elliptical, conspicuously spinulose, up to 6Lm in
long axis.
P. echinosporum Nehira ex Ramrez .. . . 430
Colo nies floccose , cottony, d eepl y lanate , at first white and at m aturity
remaining so or becoming pale gray-gree n dep ending upon th e species; colon y
rever se yellowish cream and agar uncoloured or nearl y so .
Conid iopho re s arising from th e substratum or from th e loose ae rial network
''1' :,( I
l o 600
11lIl
149. Penicillium camemberti 1 'hom : fig. 149 , colou r plat e 149, and SEM
pla te 25 , (bo tt om : right) .
Penicillium camemberti Thom - Bull . Bur. Anim. Ind. U.S . Dep. Agrie . 82: 33.
1906; see also Thom, Bull. Bur. Anim. Ind. U.S. Dep. Agrie. 110: 50. 1910; also
Thom, 'The Penieillia', pp. 312-313.1930; also Raper and Thom, 'A Manual of the
Penicillia', pp. 426-427. 1949; see also Samson et al. Antonie van Leeuwenhoek.
43: 342-344. 1977.
Synonymies
Penicillium aromaticum 111 Sopp - Z. Bakt. Parasitenk. Inf . Hyg. Abt. 11. 4: 161.
1898.
Penicillium album Epstein - Areh. Hyg. 15: 360. 1902. (Non P. album Preuss Linnaea. 24: 135. 1851, 01' P. album Rivolta - Paras. Vgt. p. 452. 1873).
Penicillium epsteinii Lindau apud Rabenhorst - Deutsehl. Krypt. Flora, Pilze Bd.
8: 166. 1907.
Colon ies upon C A (colour pIa te 149 , A a nd a) a ttain ing a di ameter ofa bo u t
1::
LwJ..l=J
10pm
O sO
0 0 0 0
0 0 0
0 0 0
00 O (l{ .
lolo n ie upon C YA (co lour plat c 14-9, B ami h ) a tta in ing a di amct cr 01'
uho u t 40 mm in 14 d a ys a l roo m tcmporutu rc (20 -25 C), sim ilar in tcx tu re
nnd cu ltural charac tc ristics lo th osc grow n upon C A, hut m ore deepl y Iloccosc ;
hc u cr spo ring in vc ry palc grccn shadcs; odo u r ca rthy ; ex udate as minu te
. Iro plcts cn mcshe d in the mycclial felt ; co lo ny rcve rse radiatcl y wrinkled
(colou r plate 149 , b) , developin g yello wish crcam shades; no coloured pi gment
d ifTu sin g into th e su rro u nd ing agar; penicilli esse nt ially as d escrib ed aboye.
Colonies upon M EA (co lour pla te 149 , C a nd c) a ttain ing a diameter 01'
nbo u t 30 mm in 14 days a t room te m perat ure (2 0 -25 C), sim ilar in texture
.md cultu ral characte r ist ics to th e preceding , bu t spo ring m ore ab u n dan tIy in
pastel gree n shades (Sguy, PI. XXVII, 400 ); odour an d ex u date la cking; no
roloured pi gment diffu sing in to th e su rrou nding agar; colony reverse (co lou r
pla te 149 , c) in yellow ish cream shades; grow ing m argin su r ro u nded b y a
frin ge 01' su b merged hyphae extending abo u t 2 mm beyond the ae r ia l gro wt h ;
pcn icilli esse ntia lly as dcscribed a boye but with the stipes 01' th e co nid iopho res
a pp caring m ore co nsisten tIy roughe ne d than u pon CA a n d C YA .
T he species description is ce n te re d upon th e typ e st rain ATCC 10 ,4 23 = NRR L
1177 = C BS 299 .48 = IJ FM 7217 , isolated by C h . T ho m in Storrs , Con necticu t,
USA , in 1904 fro m Frenc h Camembert cheese . T he strain h as re maine d in
con tinuou s laboratory cu ltivatio n since th at time wi th out a ppare nt chan ges in
cultural ap pearanc e 01' dct ail s 01' m orphology .
150. Penicillium griseum Bonorden : fig . 150 , colour pia te 150 , a n d SEM
p ia te 26 , (top : left) .
Penicillium griseum Bonorden - Abh. Geb. Mykol. p. 92. 1864. See also Thom,
'The Penicillia', pp. 563-564. 1930.
Colo n ies upon CA (col our pIa te 150 , A a n d a ), growing r apidly , attaini ng a
diame te r 01' 55 mm in 14 d ays a t roo m te m pe rature (20-25 C) , co ns isting 01' a
cIose-text u re d b asal felt overlaid by a deeply floccose aerial m ycelium infl uencin g greatIy th e colony a p pearanc e , with ce n tre irregul arly r ai sed b y tufts 01'
whi te vegetative hyphae ; thin eve n m argin surrounded by a narrow fr inge 01'
subme rge d h yphae cx te n d in g a bo u t 5 mm beyond th e ae r ial gro wth; hea vily
spo r ing throu ghou t in pal e gray-g re en shade s, but with co n id ia l areas m ostIy
obs cured by the wh ite ove rg ro wth 01' floccose vegetativ e hyphae ; colony surface
dcvcloping pale green shades with the rip ening 01' co n id ia (Sguy, Pi s. XXI ,
3 10, a nd XX , 299) ; odo u r fa int, ' m ou ld y ' ; ex u date limited in a mou n t as
m in u te d roplet s enmeshe d in the mycelia l felt; n o coloured pi gment d iffus ing
into the su rro u nding agar; colo ny reverse (colour pla te 150 , a) develop ing
ye llow-orange shades ; penicilli (fig . 150 , A) ty p ica lly asym metrical , co n sisting
01' two- 01' three-st a ge branch ed stru ctures, with all c1emen ts 01' th e con id io-
--:
~~
..
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'
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::: u....u..l=J
:.:;
::~ 10 jrm
ffi
".:'
.:':".
.'
.'
:::1
1);
~~;
o:::
1(:::
."
;' O B
.::
:~:'
::'
,','
:.;
~~:.
<'1
~~,
~!': 08
!Y
7!
::
( O 0 00
0 0 0 0
0 0 O
.~
,:.
K.
1rl1r
100jJm
::
Fig. 150. Camera lucida
drawings of Penicillium
griseum Bonorden, ATCC
20,068. A, Detailed drawings
of the penicilli. B, Mature
conidia. C, Habit sketches.
phore except phialides , conspicuo usly rou ghen ed ; conidiophore s arising from
sub merged hyphae and from aerial hyphae , usually 100 -150 tm by 2.5- 3.5
tui , septate , with api ces enla rged in a vesicle up to 5- 6 tm in diamet er; rami
when prese nt upto 20 tm by 3 .0 ustv ; metulae in groups of2- 3 elements in th e
verticil , of unequal length , com monly 11-18 tm by 2.5 - 3 .5 tm; phi alides
bottl e shape d, in clu sters of 2-6 elements in th e ve rticil, 9-1 2 tm by 3- 4 tut,
tap ering to a short neck ; con id ia globose to subg lobose , sm ooth walled or
nearly so (fig . 150, B, and SEM plat e 26, top : left) , 3 .5-5 .0 tm in diamet er;
conid ial chains forming loo sely parallel short colu m ns (fig . 150 , C ).
Col oni es up on C YA (colour plate 150 , B and b) attaining a diameter of
about 50 mm in 14 days a t room temperature (20 -25 C) , con sisting ofa close
textured b asal felt , strongly sulca ted by numerous radial furrows and slightly
raised in th e colony centre , a ppearing deepl y velvety to subla nate, with th e
colony surface ove rlaid by a den se netw ork of dirty white-coloured aerial
vegetative hyphae influen cin g greatl y th e colony appeara nce; thin membra
nous m ar gin , su rrou nde d by a fringe of submerged hyphae exte nd ing about 1
mm beyond th e aer ial gr owth ; medium sporing in the same shad es as described
aboye; with co uid ia l ureas obs cured by thc dense whit e ovcrgrowth ; exudale
nnd odour lacking; no colou red pigm cnt dillusin g into the surrounding agar;
colony rcv crs c (eolour plat e 150, b) in yellow-orange shades; penieilli as
described above .
Colonies upon MEA (colour platc 150, C and c) attaining a diameter of 50
111m in 14 days at room temperature (20- 25C) , consisting of a looser textured
basal felt, not furrowed, but raised in the centre, with thin margin surrounded
by a wide fringe of submerged hyphae extending about 4 mm beyond the aerial
growth; colony surface partially overlaid by a white overgrowth of densely
woven aerial hyphae ; sporing sparingly in marginal areas and heavily in the
centre in blue-green shades (Sguy, PI. XXIX, 429) ; exudate and odour
lacking; no coloured pigment diffusing into the surrounding agar ; colony
reverse (colour plate 150, c) developing pale orange shades in the centre and
pale cre am to colourless elsewhere with a green cast showing through ; penicilli
essentially as des cribed aboye .
The species description is centered upon the type strain ATCC 20 ,068 = IJFM
7206, isolated from soil. The type strain , probably lost, was reported by
Bonorden as isolated from rotten leaves in 1863, in Westphalia , G ermany .
Furthermore, it was so poorly des cribed by the author as to make th e identifi
cation impossible.
' I':':ll
o
'. 1
"
', } lO}lm
.'
-"..,
~r"" 1\1 ~f
(Y!i$!;'~~ e
\ :;;r...
1
. ~
'-------'
100 jrrn
..N8ogo0
ir
)U i~ 86~c?
oogoo
00 0
O
R .
Penicillium commune Thom - Bull, Bur. Anim. Ind. U.S, Dep. Agrie. 118: 56-57.
1910; see also Ch. Thom, 'The Penieillia' , pp . 324-325. 1930; also Raper and
Thom, ' A Manual of the Penicillia ', pp. 439-440. 1949.
Synonym
Penicillium fusco -glaucum Biourge - Monographie, La Cellule. 33: 128-130. 1923.
See also Thom, 'The Penicillia', pp. 325-326. 1930.
tcxturcd basal Ielt , decply llocco sc, ah out ;OO to 700 1L1lI d ecp, zon at c, su lca icd
by few sh allow radial lurrows: with whit c cvc n m argin surroundcd b y a fringc
01' sub mc rged hyph ac extc nd ing a bo u t :~ mm bcyond th e aerial growth ; colony
s u rface gradually raised towards the cen tre; spo r in g abundantIy in zones in
diffcrcnt shades of blue-green (Sguy , PI. XXXI , 465, 463, 462), depending
on th e degree of maturity of conidia ; exu d a te colourless, as minute droplets
cnrneshe d in the mycclial felt ; odour ' m ou ld y' ; amber-coloured pigment diffusing into the surrounding agar ; colony reverse (colour pIate 151, a) developing
reddish sienna brown shades in the centre and subcentral areas and pale
cinn a m o n in the margin ; penicilli (fig . 151, A) typically asymmetrical, with
clcm ents of the penicillus arising at different levels of the conidiophore in an
irregular pattern; conidiophores varying from very short up to 500 lLm in
length by 5 .0 lLm in diameter in marginal areas of older colonies , with walls
con sp icu ou sly roughened to coarsely granular in age; rami variable , up to
15-20 lLID by 2.5-4 .0 lLm ; metulae in sm all groups of two elerne n ts in th e
verticil , usually 15-20 lLm by 3 .0-3 .51Lm; phialides few in the verticil, 2-4 per
cluster, 12-17 lLm by 3-4 tui , tapering to a conspicuous narrowed neck;
con idia elliptical to subglobose, usually 4- 5 lLm, occasionally up to 7 lLm in
long axis , with walls smooth 01' nearly so (fig . 151 , and SEM plate 26 , top :
right) ; con idia l chains tending to diverge (fig. 151 , C) .
Colonies upon CYA (colour pl ate 151, B and b) spreading broadly , attaining
a di amet er of 50 mm in 14 days at room temperature (20-25 C), consisting of
a close-textu re d basal felt, strongly sul cated by numerous a nd d eep radial
furrows, with centre slightIy raised , with the colony surface overlaid by a
floccos e overgrowth of white vegetative hyphae influencing greatIy th e colo ny
aspect ; con sp icu ou sly zonate; with thin even margin surrounded by a fringe of
submerged hyphae exte nd in g a b ou t 1 mm beyond th e aerial growth ; heavily
spo rin g throughout in various shades 01' green , brown-green and blue- green
(S guy , PIs . XXXI, 462, XXIX, 427 , XXX , 448) depending on the degree 01'
ripeness 01' conidia; odour strong, ' m ou ld y '; exu d a te abundantIy produced, as
cry stal cIear yellow amber drops; amber-coloured pigment diffusing into the
su r rou nd in g agar ; colony reverse developing deep reddish sienna brown
shades with margin cinnamon (colour pl ate 151, b) ; penicilli esse n tia lly as
described ab ove .
Colonies upon MEA (colour pl ate 151, C a nd c) attaining a di amet er 01' 30
mm in 14 days at room temperature (20-25 C) , consisting of looser textured
basal felt , plane, not furrowed , deeply velvety, with few floccose tufts 01' bluegray aerial hyphae in localized zones 01' the colony surface, with thin even
m argin surrounded by a fringe 01' submerged hyphae exte nd in g about 3 mm
beyond th e aerial growth; heavily sporing throughout in blue-green shades
(Sguy , PI. XXXI , 461) ; odour spicy; exud a te lacking; no coloured pigment
d iffusing into the surrounding agar; colony rev erse (colour pIate 151, c) in
yellowish cin n um o n shades w ith a greell casi sho w ing through; peuici lli cssen tialIy as descri bed aboye .
T he spec ies descri ptio n is ce ntcrcd lIpon th e ty pe strai n c ss 3 11.48 = C MI
39,8 12 = ATCC 10,428 = NRRL 89 0 = I FO 5,763 = Q M 1,269 = IJ FM 386 7,
whic h was isol a ted by C h . T hom a t Storrs, Con necticut, USA , from cheese in
1904. The cu lture h as been u n der continuous laborat ory cu lt iva t ion withou t
significan t change in cu ltural or mo rphological characteristics.
152 . Penicillium lanosum W estling: fig. 152 , colour plat e 152 , and SEM
t.cw.l.Lw.J
10
um
O
O8 O 0
00
0
O0 O 0
0
0 .0 0
O OO
" ~'l
\ \-'>~'. ~l.
~~,~'
Penicillium /anosum Westling - Arkiv Bot. 11: 55, 97-99. 1911. See also Ch. Thom ,
'The Penicillia', pp. 317-318. 1930; also Raper and Thorn, 'A Manual of the
Penicillia', pp . 431 -434. 1949.
ippea ra ncc lo th e colo ny surface whi ch is a l111 osI plano, showing a slighl
clcvation in th c cen tre , with ew shallow urrow s; cvc n margin surroundcd by a
trin gc 01' submcrgcd hyphac cxte nd ing alxiut ~ 111m bcyond the aerial growlh ;
slighlly sporing in marginal arcas in dark gr a y-green shades (Sguy , PI.
X X IX, 433) , c1scwhere , rcmaining persistently white owing to th e f1 occose
overgrowth; odour fragant, indefinite; exudate limited in am ou n t , as crystal
clca r minute colou rlcss droplets en me shed in th e mycelial felt; no colou red
pigment diffusing into th e surro u nd ing agar ; colo ny rev ers e (colour pl at e 152 ,
a ) in pale yellow to cream sh ades ; penicilli com parativcly large (fig. 152 , A) ,
usy mme trical, irregularl y ramified and tending to beco m e div ergent , with
rouidiophores arising co m m only a s short branch es from ae rial hyphae 100 to
~ O O /lm in length, less frequently from the substratum , ranging th en from 250
lo 600 /lm by 2.8-3 .3 tui, with walls smooth 01' nearly so; rami variabl e ,
usu all y 25 - 35 /lm by 2.8 -3.0 tui, arising in small clusters 01' two in th e
vcrticil, often arising low on th e co nid iophorc ; m etulae in numbers 01' 2- 3
clcme n ts in th e verticil , abou t 9-14 /lm by 2.5 - 3.5 usn, with apices slightly
cnla rged up to 4 .0-4.5 /lm in diamet er, commonly borne at different Ievels 01'
t he penicillus; phialides in clusters 01' 2-10 elements in th e verticil, a bo u t 9-11
Ilm by 3.0 -4.0 tuu ; tap ering to a conspicuous narrow ed and short neck ;
conid ia globose to sub globose , com parative ly small, usually 2 .8- 3.0 /lm in
diameter, with walls delicately gra nular (fig. 152, B, and SEM plate 26,
centre : left); coni d ial chains loosely parallel to tangled (fig. 152 , C) .
Colonies upon CYA (colour plat e 152 , B and b) attaining a di amet er 01' 60
mm in 14 days at room temperature (20-25 C) , similarly textured as aboye but
with colony surface strongly and deeply sulcat ed by numerous raidal furrow s,
and centre irregularly fold ed and raised ; thin growing m argin f1at, su rrou nde d
by a fringe 01' submerged hyphae exte nd ing a bou t 5 mm bcyond the aeri al
gro wth ; colon y surface overlaid by a dense network 01' white f1 occose aeri al
hyphae influencing greatly the colony appearance ; heavil y sporing margin al
a rea s in dark gray-green sh ades (Sguy, PI. XXIX, 44 3) , c1sewhere th e
co nid ia l are as are entire ly ob scurcd by th e d ense overgrowth ; od our fragrant ,
suggestive 01' soap perfumes; ex ud ate limited in amount , as minute colou rless
droplets en me shed in th e mycelial felt ; no eoloured pigment diffusing into th e
surrou nd ing aga r ; eolony rev erse (eolour plate 152 , b) developing pale orange
to er eam shades; penieilli essen tially as deseribed aboye .
Colonies upon MEA (eolour plate 152, C a nd e) spreading broadl y ,
att a ining a diamet er 01' about 60 mm in 14 da ys at room temperature
(20 - 25 C), eonsisting 01' a loose-textured basal felt , loosely floceose and zanat e
in m argin al a reas, more den sel y lanate in central and subeentral areas , not
furrowed , flat, with cen tre sligh tly umbonate , with very thin margin largely
su b me rge d, extending abou t 5 01' more mm beyond th e aerial growth; heavily
sporing throughout in dccp blue-gray shadcs (S guy, PI. XXXV , 519);
eQ~q
.G;>oa
.be ' o ;, "
fJ
C)
<&
() Q)
rfY
e
211
L.....-.I
100J.lm
fR.
nicillium echinosporum Nehira - J. Ferment . Toc hnol. 11: 861. 1933. See also
M.M. Kulik , 'A Compilation of Descrip tion s of New Penicillium Species ', Agric.
Handbook No. 351. Agric. Res. Ser. U.S. Dep. Agric. p . 36. 1968.
Prnicillium echinosp orum Nehi ra was isolat cd l'r ornJ a pan esc soil in 1932 by T .
Ne h ira , who prop osed (he na me P. echinosporum and described th e species
wiihou t p ro vidin g a La tin di agn osis. In ord er to validat e th e spe cies , a Lati n
d iagn osis 01' it is given below ; th e name 01' th e original a u thor is m a int ained
1I11der R ecornmendation 46 C 01' the Interna tion al Code of Bot anical No m enrl nt u r (Sta f1e u (Ed .) , 1972) .
Larin d iagnosis of Penicillium echinosporum N ehi ra ex R am rez:
Coloniae in agaro Czapekii restrictae crescunt, post duas hebdomadas ad
O-25C, 15 mm in diametro attingunt, denso texto basali coacto mycelio,
velutinae, inaequaliter prominents, radiatae plicatae; cum undulato margine; fortiter
sporulantes coeruleo viridibus colorantur, cum hyphis aeris flavis tectae sunt; odor
abest ; exsudatio adest, exiguus, in mycelio submerso; cum flavo pigmento in medio
diffluentis; reverso rubro fusco ; neque ascomata neque sclerotia visa sunt; penicilli
bivert icillati asymmetrici, cum conidiophoris glabris, brevis , plerumque tenus 50 ~m
in longitudine per 2.0-2.5 ~m in diametro, plurimum e mycelio basali vel e hyphis
reptabundis exoriuntur; rami 15-25 ~m per 2.0 - 3.0 ~ m ; metulae 10-25 ~m per
1.7-2.0 ~ m ; phialides 2-6 in numero per verticillum, 11-17 ~m per 1.8-2.5 ~m,
lanceolatae, cum langa et acum inato collulo; conidia elliptica, 4-6 ~ m per 2.5-4.0
ILm in diametro, echinata, in brevis catenis diversis formantur.
Coloniae in agaro Czapekii cum extracto levedinis, post duas hebdomadas ad
20-25C, 25 mm in diametro attingunt, denso texto basali coacto mycelio,
velutinae, radiatae plicatae , prominentes in centro, cum aequaliter margine; parum
sporulantes, pallido coeruleo colorantur; exsudatio et odor absunt; cum flavo
pigmento in medio diffluentls: reverso aurantio; neque ascomata neque sclerotia
visa sunt.
Coloniae in agaro hordeaceo, post duas hebdomadas ad 20-25C , 25 mm in
diametro attingunt, laxo texto basali coacto mycelio, lanatae, concavae in centro,
parum prominentes in margine ; e reticulo floccosis albis hyphis aeris tectae sunt;
parum sporulantes; odor et exsudatio absunt; sine pigmento diffluente in medio;
reverso aurantio. Neque ascomata neque scierotia visa sunt.
Typus cultura CSS 344.51 e solo Japonico a T. Nehira, anno 1932 isolatus esto
Nehira 's original diagnosis follows:
'The mycelium is yellow-green upon Czapek's agar and the margin is white and 2
mm wide . After 7 days, colonies attaining 10 mm in diameter. The white marg in disappears in age and the colony surface becomes dirty dark green coloured ; the
colony reverse is bright yellow, but sometimes developing brown colours with
'1'""
yellow-brown zonation in age. At 33 lJC, the colonias become very dark green In
colour with the reverse yellow to purple brown. No growth occurs at 37C .
When viewed under the microscope, some ropes of hyphae may be observed ,
2-3 .tm in diameter.
Penicilli biverticillate asymmetric , 21 to 33 .tm; metulae 8 to 9 .tm; phialides 10 to
12 .tm. Conidia elliptical, 2 to 3 .tm , with pointed ends; some conidia are globose , 2
to 2.5 .tm; with walls roughened and echinulate .'
(Translated from the Japanese by Miss Masae Yamamoto).
154 . Penicillium giganleum R oy el Sin gh : fig. 154, coIour pIat e 154, and
SE M plat e 26 , (bottom: Ieft) .
Penicil/ium giganteum Roy et Singh - Trans Brit. Mycol. Soco 51: 805-806. 1968.
Roy and Singh's original diagnosis follows:
'Colonies growing well on Czapek's agar, attaining a diameter of 11.5 mm in a
week at 25C, dark brown; reverse yellowish brown, slightly floccose and wrinkled;
hyphae branched, delicate, mostly 2-3 /Lm broad; conidiophores fairly long, arising
as lateral branches from the main hyphae, often simple but sometimes branched,
septate, 2-4 /Lm wide, smooth walled; penicilli irregular, varying from
monoverticillate with 1-4 phialides at the apex of a short conidiophore to partially
biverticillate or fairly regularly biverticillate, but not typical of the section
Biverticillata-symmetrica; metulae 2-6 in a verticil, 7-15 by 2.5-4 /Lm; phialides
divergent with swollen base, tapering abruptly to a long , slender tip , 6-20 by 1.5-3
.m . Conidia globose, in chains with conspicuous connectives, at first olive green
becoming dark brown at maturity, with prominent spines, generally 6-9 /Lm,
occasionally up to 12 /Lm in diameter, connectives 1.5-2.5 .m long, occasionally 4
/L m .
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The fungus f irst appeared on Martin's rose-Bengal med ium as a small blackish
colony which was later stud ied on Czapek's medium w ith 3% and 20% sucrose,
and on 2 % malt agar. It grew satisfactorily on these med ia but the growth was
maximum on Czapek's medium w ith 20% sucrose.'
phores , up to :)() Itlll in length by :~.() -4.() Itlll in diumct cr, usunlly smooth
wnllcd , but arising roin trailillg hyphac more 01' less co nspicuo usly granular,
Iwarillg al th cir tips small verti cils 01' m crulac, commonly 2-3 clements in thc
vcrricil, 10-15 P.nl by :~ .0 -4 .() tui, cac h bcaring a small cluster of 2-3
phinlidcs per whorl , bottle shaped , 8-13 tuu by 3.0 -4.5 tui, tapering abruptly
11' l co nspicuously long narrowed neck; occasiona1ly simple penici1li
(al'pearing monoverticillate) are also observed, and metulae arising at different
Ie 'vds 01' the conidiophore are not infrequently encountered; conidia compara1ivcly very large, probably exceeding in diameter that of a1l known Penicillium
xpcc ics , globose, strongly echinulate (fig . 154, B, and SEM plate 26, bottom:
11'1"), about 6-10 p.m 01' occasionally up to 12 ust: in diameter; conidial chains
short , loosely divergent, with conspicuous long connectives between the conidia
(Iig . 154, C); numerous coiled hyphae, not reported by previous authors have
Iicen observed in the present study (fig . 154, D), suggestive either of asco mata
i 1I i 1ials 01' nematodes traps .
C olonies upon CYA (colour plate 154, B and b) attaining a diameter of
:d)()ut 30 mm in 14 days at room temperature (2-25 C), consisting of a
lough , c1ose-textured basal felt, similarly textured as aboye but radiately
lurrowed , appearing deeply velvety to lanate, with thin even margin
surrounded by a fringe of submerged hyphae extending about 2 mm beyond
I he aerial growth; poorly sporing, with colony surface remaining persistently
white to yellowish coloured; odour and exudate lacking; pale yellow-coloured
pigment diffusing into the surrounding agar; colony reverse (colour plate 154,
h) developing orange shades; penicilli essentially as described aboye .
Colonies upon MEA (colour plate 154, C and c) attaining a diameter of 30
111m in 14 days at room temperature (20- 25C) , consisting of a loo ser textured
basal felt, deeply velvety, plane, not furrowed, with wide thin margin
surrounded by a fringe of submerged hyphae extending about 5 mm beyond
the aerial growth ; very heavily sporing in central and subcentral areas in deep
mouse gray shades, with the conidiophores arising in a dense stand from a
closely woven network of trailing vegetative hyphae, with walls conspicuously
granular; exudate limited in amount, as minute colourless droplets enmeshed
in the mycelial felt; odour lacking; reverse of colony (colour plate 154, c)
developing yellow to orange shades with a green cast showing through; no
coloured pigment diffusing into the surrounding agar.
The species description is centered upon the type strain CBS 144 .69 = CMI
132,774 = IJFM 7149, isolated in November 1967 by C .N . Singh from soil at
the Botanical garden of the Banaras Hindu University, Varanasi-5, India .
Other strains were isolated simultaneously from the rhizosphere of Brassica
campestris L., at the same locality .
Penicillium giganteum Roy et Singh is a quite distinctive species characterized
particularly by its giant, strongly echinulate conidia , hence the specific epithet .
436
haptcr 7
' I'horn ( 1949), p roduce colon ies ranging from definitely floccose to almost
vclvcty , bu t typi call y showing pa rt of the aer ial hyphae combin ed in to
.uu istomosin g ropes or fu ni clcs; with con id iophores partly ar ising from
sub rnerged hyphae , partly as terminal portions of ascen d ing ae r ial hyphae ,
.uul in part as branch es fro m th e ro pes of ae r ial hyphae , but n ever forming
rrcct fascicles or syn ne mata; with peni cil1i cons iste n tly a symmetrical , u sually
I wo- or three-stage branch ed .
O f th c two recognized ser ies (Rape r a nd T hom, 1949), n amely Penicill ium
trrrestre ser ies and P. pallidum series, only th e latter h as been retained in
lite present stu dy, since al1 sp ecies incl ude d by Raper a nd Thom (194 9) in th e
ormer have ac tual1y been re d uce d to sy no ny m ies wit h oth er for ms incl u de d in
t hc Asymmetrica-fascicul at a Subsection (Samson, Stolk, a nd H adlok , 1976) .
T he members of thc Penicillium pa llidum series do not a ppea r to b ear a clos e
relat ion ship to a ny recogn ized ser ies. They seem to represent a gr oup wel1
a pa r t from al1 othe rs.
Key to species
Page
. . . . 438
.. . . 438
.... 440
. . . . 442
. ... 444
Colonies growing rather restrictedly upon Czapek ' s agar but broadly
spread ing upon Cz apek-ye ast extrac t agar; usually rather thin, with su rface
growth somewhat funi culose; con id ial a reas variable in colou r, wh ite lo crca m ,
bu ff, light gray, or lav ender to vinaceous, never d eveloping true gree n shadcs ,
C onidiophores co m parative ly short , ge ne rally arising from ropes of acri al
hyphae ; con spicuo usly sep ta te , with wall s of con id iop ho res, includin g rami ,
m etulae , an d even phialides usuall y cons picuo usly rou ghen ed .
Penicilli u sually com paratively narrow, with all elements tending to be
closely ap pressed .
Phialides ar ra nged in com pact clu sters , closely parallel , com para tivcly
narrow , seldom exceeding 3.0 p.m in diamet er, with walls usually echinulatc.
C onidia stro ngly ellipt ica l to cylindrical, smo oth wall ed , oft en adherin g in
long cha in s.
Irrespecti ve of th e true rel ation ship th at m ay ex ist between the spec ies
included in this series, all of th em, however , possess ce rtain m arked
chara cte r istics in co m mo n whi ch enable th em to be cons ide re d together more
satisfacto rily th an with any othe r of th e R ap er a nd T ho m ' s (1949) recognizcd
senes.
155 . Penicillium pallidum S m ith: fig. 155 , co lo ur plate 155 , and SEM
plat e 26, (bottom : right) .
Synonymy
Geosmithia putterilli (Thom) Pitt - Can. J . Bot. 57: 2021-2030. 1979.
Penicillium pallidum Smith - Trans. Brit . Mycol. Soco 18: 88-89. 1933. Also Rapar
and Thom, 'A Manual of the Penicillia', p. 461. 1949.
Colon ies upon C A (co lou r pl at e 155 , A and a) gro wing restrict edly,
attaining a di amet er of 25 mm in 14 days at room te m pe rature (20 -2 5 OC ),
co nsisting of a thin, loos e-textured ba sal felt , with margin largely sub m erged ,
extend ing a bo u t 5 mm beyond th e ae rial growth , plane ; m yceli al growth
giv ing ris e to a sp arse devel opment of coni d ial structures and short funiculose
tu fts or rop e of ae rial hyphae bearing con id iopho res ; colo ny su rface at first
white , becoming cream colou re d in age; odo u r and ex udate lacking ; no
colou re d pi gment diffu sing into th e su rro u nd ing agar ; colony reverse (co lou r
plat e 155, a) colou rless or in pale cream shades ; penicilli asym metric and
biverticillat e (fig . 155 , A), with co nid iopho res ar ising from tr ailing h yphae or
from rop es of h yph ae , m any borne from foo t cells as obse rved in th e gen us
-,
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;~
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L.-J
100J.lm
" <'
f.K..
Aspergillus; conidiophore walls cons picuou sly roughen ed, with metulae , rami ,
a nd phi alides similarly marked; m etulae in groups of 2- 3 elements in th e
verticil, a bou t 9-14 /lm by 2. 5- 3.0 /lm ; phialides with walls con spicuo us ly
g-ra nular, narrow, taperin g grad ually to a narrowed short neck , 8-1 5 /lm by
:.! .5 - 2 .8 /l m; conidia cylind rica l, 3.5 - 4 /l m by 1.8-2.0/lm , sm ooth walled
(Iig . 155, B, and SEM plat e 26, bottom ; right) ; con id ial chain s tending to for m
loosel y parallel colu m ns (fig . 155, C) .
Colonies upon CYA (colour plat e 155, B and b) spreading broadly , attain ing
a diamet er of a bou t 45 mm in 14 d ays a t room temper ature (20-25 OC ),
consisting of a tough , close-textured basal felt, strongly radiately wrinkled , a nd
slightly raised in th e ce ntre ; a ppearing powdery to chalky, with a thin eve n
margin surrounded by a frin ge of su b me rged hyphae ex te nd ing abo u t 2 mm
beyond th e aerial gro wth; heavil y sporing throughout in pal e cream sha des ;
odo u r and exud a te lackin g; pal e a mbe r-coloured pi gm en t diffu sin g into th e
su rrou nd ing agar ; colony revers e (colour plate 155, b) devel oping ora nge
shades; penicilli esse n tially as d escribed aboye , with co nid iop ho res usu all y
short, com monly two-stage branched , not exc eeding 175 tui: in len gth by
2.0- 3.0 /lm in di amet er , arising primarily from the basal felt a nd from ro pes of
ae rial hyphae.
C oloni es upon MEA (co lour plat e 155, C and c) attain ing a diamet er of
abo u t 45 mm in 14 days a t ro om temperature (20 -25 OC ) , con sisti ng of a
't'I'\J
looscr tex tu red ba sal fclt, co ns picuo usly funi cul ose all ovcr thc colo ny surfa cc ,
flat , not furro wed , slightly urnbon at c in the centre , with thin marg in
su rro u nde d by a fringe of subme rged hyphac cxtc nding abo ut 5 mm bcyond
the ae rial growth ; heavil y spor ing throughout in Naple yellow shades (Sguy ,
PI. XIII , 190), wit h conidiop ho res short, a rising mostly from ro pes 01' hyph ac ,
giving to th e colony surface a chalky appearance; odo u r and exudate lackin g;
pale yellow coloured pigment di ffus ing into th e su rrou nding agar; colony
reve rse (colour plat e 155, e) developing pale orange shades; pe n icilli esse ntially
as described .
T he species desc ription is centered up on ou r notes on Sm ith's type strain c as
260.33 = C M I 40,214 = ATCC 10,4 78 = NRRL 2,037 = I Fa 5, 758 = QM 1958,
isolated fro m cotto n ya ro, Eng land, 1931, a nd upon strain C BS 248 .32 = N R RL
2,156 = IJFM 7118, isolat ed in 1932 in H olland fro m an elm beetl e (Scolytus sp. ) ;
the latter strain prob abl y suffers a vitamin deficien cy , clearly shown by the
very res tr icted growth upon CA (colour plate 155, A) and the rapid growth
upon CYA (co lour piate 155, B) . H owever , th e typ e strain 01' Penicillium
pallidum, as noted in Smith's original diagn osis (1933) is characterized by
broadly spreading colonies upon CA, attaining a diamet er 01' 65- 70 m m in 14
days at room te m peratu re, showing numerous radial furrows, bei ng simi la r in
texture and cultural appearance to strain C BS 248.32 cultivated upon CYA
(colour pla te 155, B) .
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(from the stipe to the phialides) (fig . 156, A); conidiophores mostly arising
Irorn ropes of hyphae, commonly short, less than 100 11m by 2.5- 3.5 11m ;
rami , in small groups of one or two elements in the verticil, about 15-25 11m
by 3.0- 3.5 11m; metulae n umbering 2-3 elements in the verticil, 12-1 8 11m by
2 .5- 3 11m ; phialides in closely appressed who rls, numbering 5- 10 elements in
the verticil, cylind rical, conspicuously wa rty, 9.0-15 .0 11 m by 1.5- 3.0 11m ;
con id ia cylin d rical, 4.0-6.5 11 m by 1.5-2.5 11m , smooth wa lled (fig . 156, E,
and SEM plate 27, top : left); conid ial chains forming fairly well-d efin ed
colu m ns (fig. 156, C).
Colonies upon CYA (colour plate 156, B a nd b) spread ing broadly, attaining
a diamet er of about 60 mm in 14 days at room temperature (20-25 OC ),
consisting of a thi n, loose-textured basal fe1t, radiately furrowed and raised in
the centre in a crateriform pattern, wit h very thin margin surrounded by a
wid e frin ge of submerged hyphae extend ing about 4 mm beyond th e aerial
growth , with growing surface ov erlaid by numerous ropes of aerial hyphae
lending a powdery appearance to the colony ; cons picu ou sly zonate; heavily
sporing throughout in Naple yellow shades ; exudate and odour lacking ; no
colou red pi gm cnt difu sin g' inro th c su rro u nd ing aga r ; colo ny rev e rso stron gly
pulling th c agar away rom th e cu ltu re pl a te , a llow ing good sporu la tio n
underneath in a reas where th e a ir has becn a vaila b lc th rou gh th c a gar cracks
(colour plate 156, b) ; penicilli esse ntia lly as des cribed aboye.
Colo n ies upon M EA (col our plat e 156, C a nd c) growing r ather restrict edly ,
a ttain ing a di amet er 01' a bou t 30 mm in 14 da ys a t room te rnpe rature
(20 - 25 OC ) , co ns isting 01' a thin , loose-textured ba sal felt , pl ane , za nate , not
fu rrow ed , with a powdery a p pearance, with a ye ry thin m argin surro u nde d by
a frin ge 01' su b merged h yphae ex te nd ing a bou t 2 mm beyond the a erial
gro wth , with th e funiculose habit re d uced 0 1' la ckin g ; heavil y sp orin g
th ro ughou t in N aple ye llo w shades , wi th co n id io pho res m ostl y a r ising from the
su b st ratu m in a dense stand , wi th co ni d ia l chains producing fairly well-defined
colu m n s up to 200 /lm long ; odou r a n d ex u date lacking ; no co lo ured pi gment
di ffu sing into th e su r ro u nd ing agar; colo ny reverse (co lo u r pl at e 156, c)
develop ing ye llo w-o ra nge sh ades .
T he spec ies description is ce nte red upon strain C BS 233 .38 = C M )
40 ,212 = NR RL 2,024 = IJFM 7182 , isolated b y J .C. N eill in N ew Zeal and , from
Beilschmiedia tawa-discoloured timber. This cu ltu re a ppare ntly differs from th e
or iginal ty pe strain describ ed by Thom , in 1930 , from a cu lture se nt to him by
F. M . Putterill , Cape T own , So u th Afri ca , a nd quickl y lost from hi s collection,
only in producing co n id ia that a re m o re co ns iste nt ly wh ite , h ence a p proach in g
Penicillium pallidum in cu lt ural a p peara nce.
157. Penicillium namy slowskii Zaleski : fig. 157 , colour pIa te 157 , and
SEM plate 27, (top : right).
Penicil/ium namyslowskiiZaleski - Bull Acad. Polonaise Sci . Math. Nat . Sr. B. pp.
479-480. 1927. See Thom, 'The Penicillia', pp. 484-485. 1930; also Raper and
Thom, 'A Manual of the Penicillia', pp. 462-464. 1949.
Synonym
Geosmithia namyslowskii (Zaleski) Pitt - Can. J. Bot . 57: 2021-2030. 1979.
Colo n ies upon C A (colour pi a te 157, A) gro w ing ye ry restrict edly , a ttain ing
a di amet er 01' 15 mm in 14 da ys at room temperature (20- 25 OC ) , co ns ist ing 01'
a very thin b a sal fel t , wholl y su b merged, almost transp arent , producing
limited con id ia l structures , m ostl y near th e colony ce ntre, dirty white to
eolou rless; odour stro ng, ear thy; ex udate la cking ; co n id ia l str uc tu re s sparse ly
p ro d uce d, w ith co ni d iophore s ge nerally arisin g fro m su b merg ed h yphae , ereet,
com pa r a tively co arse, with walls co ns picuo u sly a n d coarsely ro u ghe ne d (from
th e stipes to th e phialides in clusiv e) .
C olon ies upon C YA (colour pl ate 157 , B a nd b ) growing lu xuriantl y a n d
1':'
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Fig. 157. Camera lucida
drawings of Penicillium
namyslowski Zaleski, CBS
353.48. A, Detailed draw ings
of the penicilli. B, Mature
conidia. C, Habit sketches.
11'1
first cylind rical, becomin g lat cr cllipt ica l , :~ .5 -5 . 0 by 2.B-:1. 5 J.tlll in d iamc tc r,
with waIls smooth (lig. 157, B, an d SE M plato 27, (0)): righ t); odo ur stron g,
sugges tive of the Beta uulgaris L. a ro ma; ex udare lim itcd in a mou n t , as minute
colou rles s dropl ets en mes hed in th e m yceli al felt ; no colou rcd pigm ent
d iffus ing into th e surro u nd ing agar; colon y rever se (co lour pl at e 157, b)
developing oc h rc shadcs.
Colon ies upon M EA (co lour pIate 157, C a nd c) spread ing broadly,
atta ining a di amet er of 60 mm in 14 days at ro om te m perature (20- 25 OC ),
cons isting of a ver y thin , loose-textured basal feIt , flat , not wrinkled except at
th e colony cen tre whi ch is irregul arl y folded and ra ised in a somewha t
cra te riform pattern , with very thin marg in largely su bmerge d, extc ndi ng
5 m m 01' m ore beyond the aeria l growth; heavil y spo ring th ro ugho u t in the
sa me gray-gree n shades as aboye (Sguy, PI. X X IX, 427); odour stron g , as
aboye; ex udate lackin g; no colou re d pi gm ent diffusin g into the surrou nd ing
agar ; colon y reverse (colo u r pl at e 157 , c) developing yeIlowis h to oc h raceous
shades with a p ale gree n cas t sho wing throu gh ; penicilli esse n tia Ily as described
aboye, with co nid ial chains forming fairly we Il-de line d colum ns (lig . 157 , C) .
The species desc rip tion is ce nte re d upon the strain CBS 353 .48 = N R RL
1,070 = CM I 40,033 = ATC C 11,127 = Q M 1,932 = IJ F M 7202, whic h is believed to
be Zales ki' s type strain, isolat ed fro m Polish soil u nder co nifcrs in 1926.
The a boye description is in fairly goo d agreeme n t with Zales ki's orig inal
di agn osis excep t for th e cons picuously roughe ne d coni d iophores (Zales ki
re po rted and iIlust rated aIl walls as smooth). In study ing th e a bo ye strain,
Thom ( 1930), and R aper and Thom ( 1949) rep orted con id iophore wall s as
ro ug h. The culture received by us from the Cen traalb u reau voo r Schimme lcultu re s, Baarn , T he N etherl ands, re ta ins th e characte r observed b y T ho m
and is believed to re present Zaleski' s original isolat e despite the re po r ted
differ en ces in th e .character of th e con idiopho re waIls.
158 . Penicillium lavendulum R aper el Fennell: fig. 158 , colour pl at e 158 ,
a nd SEM plat e 27, (ce n tre : left) .
Penicillium lavendulum Raper et Fennell - Mycologia. 40: 530-533. 1948. See also
Raper and Thom, 'A Manual of the Penicillia', pp. 464-465. 1949.
Synonymy
Geosmithia lavendula (Raper et Fennell) Pitt - Can. J. Bol. 57: 2021-2030. 1979.
C olonies upon C A (co lou r plate 158, A and a) attaining a di amet er ofabo ut
40 mm in 14 d ays at room temper ature (20 - 25 OC ), co ns isti ng of a loosetex tured b as al felt, plane 0 1' nearly so, comparatively thin , with irregul arl y
d issect ed m argin surrou nded by a fringe of su b merged hyphae extend ing abo u t
'r' r d
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146
diameter , with wall s srnooth (Iig . 1:>8, B, a nd SEM plat e 27, ce ntre; lcft);
con id ial chains tending to adher e int o loose colu m ns,
C olonies upon C YA (colour plate 158, B a nd b) spread ing broadly , attainin
a di am eter of 55 mm in 14 days at room temperature (20 - 25 OC), co ns isting 01"
a close-t extured basal felt , strongly funi cul ose , com pa ra tively thin , radiatc1y
furrowed a nd somewhat rai sed in th e cen tre , with thin eve n m argin
surrou nde d by a frin ge of su b me rge d hyphae ex te nd ing abou t 3 mm beyond
the aerial growth; poorly sporing in dirty white shades, never showing
lav ender colou rs; odo ur lacking ; exudate limited in am ou nt , as colou rless
d ropl ets enme she d in th e my celi al felt ; pale yello w-coloured pigm ent diffusin g
int o th e su rro u nd ing agar; colony rever se (colou r plat e 158 , b) devel opi ng
reddish brown shades; peni cilli esse ntially as described aboye .
C olonies upon MEA (colour plat e 158 , C and e) att a ining a di amet er 01"
30 mm in 14 days at room temperature (20 -25 OC ), consisting of a loosetextured basal felt , conspicuo us ly funi cul ose with a cotto ny or velv cty
appeara nce , with a thin m argin surrounded by a frin ge of sub me rge d hyphae
exte nd ing abo u t 2 mm beyond th e ae rial growth, and centre slightly
umbon at e ; colony surface plan e , heavil y sporing throu gh out in cora l pink ,
sha des (Sgu y , PI. lII , 33); odo ur and exudate lacking; no coloured pigment
diffu sin g int o th e surro und ing a ga r; colony reverse (colour plate 158 , e)
developing ora nge to brown shades with a gree n cas t sho wing th rou gh ;
peni cilli esse ntially as dcscribed aboye but with m ost con idio pho res aris ing
fro m ro pes of h yph ae .
The- spec ies description is ce nte red up on th e type stra in CBS 344, 48 = C M I
40,5 70 = ATCC 10, 463 = NR R L 2, 146 = I F 7,729 = Q M 1929 = IJ FM 7113, isolat ed
in lul y 1947 as a laborat ory conta m ina nt by K .B, R aper a nd D.1. Fennell.
The correc t placement of thi s spec ies remain s somewha t ope n to qu estion
since it typi call y devel op s m or e irregularly and com plexly arrange d penicilli
th an othe r spec ies included within th e Penicillium pallidum series. T he spec ies is
appa rc ntly uncommon in n ature; Penicilliu m lavendulum has been rep orted twic e
as far as we know : th e type stra in and stra in CBS 582 .6 7, isolated from woo d of
Carya sp. by R . K enneth in 1967.
T he members of th e Penicillium pallidum series, characterized by th eir
appressc d roughe ne d peni cilli , th eir lon g cylind rica l, ro ughe ne d phi alides
with out a conspicuous ne ck and abse nce of green colours sho w a certain
resembl an ce a nd affin ity to P. cylindrosporum Sm ith a nd P. argillaceum Stolk,
Evans el Nil sson of th e su b -sec tion Asymmetrica -divari cat a .
haptcr 8
Asymmetrica-fasciculata sub-section
.... 451
.. .. 491
.... 498
. . .. 486
14
l.w.J.!WJ
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McCulloch and Thom, J. Agric. Res. 36: 217-224. 1928. Independently published
by J.E. Machacek under the same specific name, Ann. Rep. Quebec Soc o
Protect. Plants. 19: 77-86. 1928; also Thom, 'The Penicillia', pp. 381-383. 1930;
also Raper and Thom, 'A Manual of the Penicillia', pp. 471 -475. 1949.
Colon ies upon CA (colour piate 159 , A and a) growing rather res trictedly,
attaining a diameter of about 25 mm in 14 days at room temperature
(20- 25 OC ), consisting of a fairly tough basal felt, with central area u sually
raised and deeply floccose, and wit h marginal area radially sulcated by shallow
fur rows, and surrounded by a very narrow fr inge of submerged hyphae
extending less than 1 mm beyond the aerial growth . Scierotia characteristically
produced, especia lly in recent isolates, varying in number in different isolates
and depending upon environmental factors, particularly temperature ; u suall y
produced at temperatures of 25 oC and aboye, scantily at 15 " C and below ;
sporing abundantly in blue-green shades (Sguy, PI. XXX, 438, 443) partly
obscured by the deep floccos e overgrowth of wh ite ae rial hyph ae ; exudate and
odo ur lackin g ; rcddish brown -col ou rcd pi gmcnt diffu sin g int o the su rr o u nd ing
uga r; eolony re ve rso (colour plat c 159 , a) devcloping rcdd ish bro wn shades; in
rccent isolates sclero tia norm all y appeari ng in abo u t 6- 7 days a nd developing
in succ ess ive conce ntric zo nes 0 1' as sec tors in locali zed areas, va ry ing greatly
in size , usu all y rangin g fro m 150 to 300 p.m but sometimes up to 500 p.m in
di ameter, a t first cream to pale pinkish tan, in age becoming tan 01' very pa le
br own , smooth, with walls co ns isting of th ick-w alled cells 8- 12 p.m in
d iameter; con id iophores aris ing primaril y fro m the substra tu m , m ostly as
independe nt structu res 0 1' m ononematou s but ofte n more 0 1' less aggr egated
in to definite fascicles, with wa lls eithe r smooth 0 1' ro ughe ned; gene rally few ,
scattere d, a nd in con spi cu ou s among th e sclerotia in new isolates , in older stock
cultures more abu ndant a nd co m mo nly aris ing from within th e floccose felt ,
often very long (u p to 2 m m ) and abo u t 2.5-4 .0 p.m in di ameter ; peni cilli
com paratively large , usu all y cons isting of one - 0 1' two -stage branch ed
stru ct ures (fig. 159, A), with 0 1' without one 01' two rami 20- 25 tut: by
2.8 -3.5 tut, bearing verticils of few m etulae 10-1 5 p.m by 2.8- 3.5 p.m ;
phialides 2-5 in th e vert icil, 9 - 12 p.m by 3- 4.0 tun , tap ering to a short nec k;
conid ia su bglobose, smooth wa lled 01' nearly so (fig. 159, E , and SEM piate 27.
centre : ri ght) ; 3.5 -4 .0 p.m in long ax is; con id ial cha ins often adheri ng in lon g
cha ins, more 0 1' less loosely parall el 0 1' tangled (fig . 159, C).
Colon ies upon C YA (colour plat e 159, E and b) spread ing broadl y , at ta ining
a d ia me ter of 55 mm in 14 day s at room temperatu re (20- 25 OC ), cons ist ing of
a deepl y floccose felt , radiately furrowed and rai sed towa rds th e centre , with a
thin eve n m argin surrou nde d by a narrow fri nge of sub me rge d hyph ae
extend ing 1 mm beyond th e ae r ial growth; sporing abu ndantly in mo use gray
shades (Sguy, PI. XXX V , 523), especially in marg inal zones; odo ur lack in g;
exudate ab u ndant , as la rge yellow a m be r-co lou re d drops; am ber-colou red
pigm en t di ffu sin g into th e surro u nd ing agar ; colony reverse (colour piate 159,
b) in reddish cinriamon shades; peni cilli essentially as described abo ye;
sclerotia as described abo ye, not produced in old stock cultu res.
C olonies upon M EA (colour plat e 159, C and c) attaini ng a d iame ter of
50 mm in 14 days at room temperature (20- 25 OC ), consis ting of a looser
tex tu red basal felt , plane , not fu rrowed , with centre slightly umbonat e , thinn er
th an whe n on the aboye su bs trata, floccose , with thin m argin surrou nde d by a
wide fringe of subme rge d h yphae ex tend ing abo u t 4 m m beyond the aerial
growth ; abu ndan tly spo ring in old stoc k cultures in pale yellowi sh shades
lending a gra n ula r appe arance to the colony su rface ; in recen t isolat es,
abundant p rodu cti on of sclero tia whi ch gene rally dominat e th e colony as pec t ;
odour fruity , aromatic; exudate lacking ; no colou re d pigm ent d iffus ing int o th e
surroundin g agar; peni cilli essentially as described above.
The spec ies is ofte n found to be th e ca u se of the ro t in gla d iolus corms .
The spec ies description is ce ntere d prirnarilv on the type strain C BS
1!i4
9 :~ 9
Penicillium verrucosum Dierckx - Ann . Soco Sci. Bruxelles. 25: 88. 1901.
O UTSTAN DlNG FEATURES
160 . Penicill ium verrucosum Di erekx val'. cyclop ium (Westling) Sa mso n,
Stolk, el H adlok: fig. 160 , eolour plate 160 , a nd SEM plat e 27,
(bottom: left) .
Penicillium verrucosum Dierckx var. cyclopium (Westling) Samson, Stolk et Hadlok
- Studies in Mycology. 11: 37-41. 1976.
Synonymies
?Penicillium aurantiogriseum Dierckx - Ann. Soco Sci. Bruxelles . 25: 88. 1901.
?Penicillium puberulum Bainier - Bull. Soco Mycol. Fr. 23: 16. 1907.
Penicillium biforme Thom - Bul!. Bur. Anim. Ind. U.S. Dep. Agric. 118: 54. 1910.
Also Raper and Thom, 'A Manual of the Penicillia', pp. 437-439. 1949.
Penicillium cyclopium Westling - Ark. Bot. 11: 55.1911. Also Raper and Thom,
'A Manual of the Penicillia', pp. 493-497. 1949.
Penicillium palitans Westling - Ark . Bot. 11 : 55. 1911. Also Raper and Thom, 'A
Manual of the Penicillia', pp. 488-489. 1949.
~ ,;
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Colon ies upon C A (colou r pi a te 160, A and a) usu all y sp read ing b ro adl y ,
att aini ng a di amet er of 45- 50 mm in 14 d ays at room te m pe rature
(20-25 OC ), sometimes gro wing slower, with a di amet er of 30-3 5 mm ; in
recent isolates strongly granular with m any fascicula te con id iop ho res
p articul arl y in marg inal areas, which are thin and su r rou nded by a frin ge of
sub me rged h yphae ex te nd ing about 3- 4 mm beyond th e aeria l growth;
fascicul ati on is u su all y arranged in co nspicuo us conce n tric zo nes; in sorn e
strains th e colo ny su rface is almost velve ty or floccose , but sho wing sorne
fascicul ation ; heavil y spo ring through out in gray-green to dull blue-green or
bright blue-green , ran gin g from pal e blue to gray -blue shades, ap p rox imating
arte m isia, lily , celandine , gnaphaliu m, sage-gree n , (Sguy, PIs . XXVII , 404,
401, XXX, 448, 447 , 446, XXXI , 463, 462, 461) with th e ripening of con id ia ;
ve geta tive ae rial h yphae m ostly a bsent, in sorn e strains yellow ish or h yaline ;
ex udate m ostl y present in recent isolates as colou rles s droplet s; odo u r st rong,
earthy or m ouldy , som etimes aro matic, fruity , u su all y a p ple-like, th ough in a
particul ar strain , IJ F M 3865, isolated by us as a culture con ta m inan t in our
lab oratory , M ay 1977, (sce co lo u r plat o 167 , a nd lig. 167, p. 471) a fra gr ant od ou r
suggestive of Anan s satiuum wa s dct cci cd , th e co nidio phore wall was srn ooth ,
and a bright ye llow pigment was diffu sin g into th e su rro u nd ing agar upon C A
a nd CYA (colou r plate 167, A and B). H owever, this str ain duplicated th e type
strain in details 0 1' morphology . Colony re verse (co lou r plat e 160 , a) ora ngeb rown , yellow, pinkish , purplish to ligh t m a ro on , dep endin g upon th e strain;
yellow-colo ured pi gm ent some times diffusin g into th e surrou nd ing agar.
Penicilli la rge (lig. 160 A), a bo u t 50-60 fAm in len gth , asy m metric, bearing
loosely parall el conid ial cha ins to tangled chains 0 1' co nid ia in irregul ar m asses
up to 150 fAm lon g (fig. 160, C ), with con id iophores arising from th e
subs tra tu m, m ostl y 200-400 fAm in length b y 2.8 -3 .5 fAm in diamet er,
sometimes coarser , with wall s typi call y ro ughe ne d but in sorne strains
ap pearing smooth 0 1' nearl y so (lig . 167 , A); penicillus showing one 0 1'
occasionally m ore ram i, 20-30 fAm by 3.5 tun , 0 1't en ap p ressed, u su all y
bearin g sm all clusters 0 1' 2-4 m etulae 12-17 tui: by 2.4 -4.0 tuu; each
su ppo r ting a wh orl 0 1' 4-8 phialides, abou t 9- 12 fAm by 3.0-4 .0 ust , bottle
shaped, taperin g ab ru ptly to a very sho rt neck; co nid ia globose to su bg lobose,
mostly 3-4 .5 fAm in d iam et er, with wall s smooth 0 1' delicat ely roughened (lig .
160, B, and SE M plat e 27, bottom: left ); conid ia l chains usu all y loosely
parallel to tangled , some times forming well-de fine d di vergent co lu m ns (lig .
167, C).
C olonies u pon C YA (colour pl at e 160, B a nd b) attain ing a di amet er 0 1'
60 mm in 14 d ays at roo m temperature (20-25 OC ), sim ila r in texture as noted
aboye but heavier spo ring throu gh out in da rk er shades 01' gree n (Sguy, PI.
XXIX, 431) with coni d ial zo nes arranged in co nce n tric areas; odo ur mouldy ;
ex udate en meshe d in the m ycelial felt ; a mber-colou red pi gm ent diffusin g into
th e su rrou nd ing agar , in sorne strains (co lour pl at e 167, B) bright yellow
pi gm en t d iffus ing into th e su rrou nd ing agar; colony reve rse in reddish brown
(co lour plat e 160 , b) 0 1' yellow shades (colour pla te 167, b); penicilli esse nt ially
as described a boye.
Colonies upon M EA (colour plat e 160, C a nd e) sp re ad ing b roadl y ,
a ttaini ng a di ametcr 01' 65 mm in 14 d ays at roo m temperature (20-25 OC ) ,
co ns isting 0 1' a looser text u re d basal felt, plane , not furrowed , co ns picuo usly
zo nate, a ppearing more 0 1' less gra nula r, showing co nce n tric zo nes 0 1' fasc icles;
with thin margin surro unde d by a fringe 0 1' su bmerged h yphae ex te nd ing abou t
4 mm beyond th e ae rial growth ; heavil y sp oring th ro ugho u t in very deep bluegree n shades (Sguy , Pi s. XXIX , 43 1, and X XX , 446) ; odour penetrating ,
cart hy to m ouldy; exudate la cking; no coloured pigm ent diffusin g into th e
su rrou nd ing agar ; colony reverse colou rless 0 1' developin g orange shadcs;
pe nicilli as d escribed a boye but with th e con id iop hore wa lls more coarsely
ro ug he ned .
Sp ecies description is ce nte re d up on strain C BS 161.42 = CM ! 39,803 = ATCC
11 ,507
NJ{RL
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Penicillium verrucosum Dierckx varo verrucosum Samson, Stolk et Hadlok Studies in Mycology. 11: 34-35. 1976.
Synonymies
Penici//ium verrucosum Dierckx - Ann. Soco Sci. Bruxelles. 25: 88. 1901 .
Penicillium musae Weidemann - Z. Bak. Parasitenk. Abt. 11, 19: 687. 1907.
Penicillium viridicatum Westling - Ark. Bot. 11: 53. 1911.
Penicillium stephaniae Zaleski - Bull. Acad. PoI. Sci. Sr. B. pp. 451-452. 1927.
Penicillium b/akes/eei Zaleski - Bull. Acad. PoI. Sci. Sr. B. pp. 441-444. 1927.
Penicillium psittacinum Thom - 'The Penicillia', p. 369. 1930. Also Raper and
Thom, 'A Manual of the Penicillia', pp. 448-449. 1949.
Penicillium /anoso-viride Thom - 'The Penicillia', p. 314. 1930. Also Raper and
Thorn, 'A Manual of the Penicillia', pp. 434-436. 1949.
growth ; hca vily spori ng throu gh out in decp hlu c-grccn shude (S guy, PI.
XXX, 442, 447) ; limit ed clca r exu da re pr oduccd ; odour stro ng, 'ca rthy";
pcni cilli more consisten tly asym mc tric an d la rger but ot he rwise as on C A; no
coloured pigm cnt d iffus ing int o th e su rrou ndin g a ga r ; colony reverse (colour
plat e 161, b) dev eloping pale yellow ish orang e lo reddish brown shades.
Colonies u po n M EA (colour pla te 161, C and c) atta ining a di ameter of
about 55 mm in 14 days at room tempera ture (20-25 OC ), consisting of a
looser textured basal felt , com parativ ely thi n , appearing velvety,
conspicuously zonat e , showi ng zo nes of fasc icula te conidiopho res, especially at
th e marg in , whic h is thin a nd su rro u nded by a frin ge of subm erged hyphae
cxte nd ing abo u t 5 m m beyond th e ae rial gro wth ; heavily sporing throu gh ou t
in dee p grecn colours (Sguy, PI. XXVII , 401); ex udate not produced ; odo ur
strong, ' earthy ' , suggest ing act ino mycetes; penicilli m ore abundantly
produ ced , esse ntially duplicat in g th ose u po n C A and CYA but with
con id iophores m ore cons picuously rou ghened ; no coloured pi gm ent diffu sin g
int o th e su rro u nd ing agar ; colony rever se (colou r pIale 161, e) developing
red dish brown shades .
Species descr iption is ce nte red prim arily upon strain CBS 265 .29 = ATCC
10,116 = NRR 932 = IJ FM 3864, isolat ed by Ph . Biourge as P. aureum Corda, and
stra in C BS 603 .74 = NR R L 965 , isolated by Biou rge and cons ide re d as th e
neotype of Penicillium verrucosum Di erckx .
P. oerrucosum val'. verrucosum ca n be di stingui shed from th e othe r va rieties by
its com m only restricted gro wth (ge ne rally Icss th an 30 mm in d iameter within
14 days), a nd its bri ght yellow -gree n colour upon CA. Althou gh th e strain CBS
265.29 (for me rly th e typ e strai n of P. psittacinus T hom) (see colour plate 161)
grows rather rapidly , the spec ies is cons ide re d by Samson el al. ( 1976) to be
identical to P. verrucosum va l'. verrucosum on acco unt of its identical
conid ioge no us structures.
H adl ok : fig. 162, colou r plat e 162, and SEM plat e 28, (to p: left).
Penicillium verrucosum Dierckx varo album (Smith) Samson, Stolk et Hadlok
Studies in Mycology. 11: 35-36. 1976.
Synonymy
Penicillium cyclopium Westling varo album Smith - Trans. Brit. Mycol. Soco 34:
18. 1951.
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the colony m argin , with colony surface almos t pl ane , sligh tly rais ed in
co nce n tric zones; odo u r lacking ; exudate not produced ; no colou re d pi gm ent
diffu sin g into th e su rrou nd ing agar; colon y rever se (colour plate 162, a)
developing cream rosy to pale ora nge shade s; 'conid iogenous structures
identical to the othe r variet ies (Fig. 162, A , B a nd C ) .
Co lonies upon CYA (co lou r pla te 162, B and b) sim ila r in cult ural
characte ristics, det ail s of m orphology and rat e of growth to th ose d escribed
up on C A but strongly radiately wrin kled, with m embranou s m argin , a bo ut
1 mm submerged ; odo u r and ex udate lacking; pal e yellow -colou re d pi gm ent
d iffusin g in to th e su rro u nd ing agar ; colon y reverse (co lour pIate 162, b)
developing pal e yellow -orange shades .
Colon ies u pon M EA (co lour plat e 162, C and c) attaini ng a di amet er of
50 mm in 14 d ays at room temper ature (20-25 OC ) , co nsisting of a looser
tex tu red b asal felt , ove rlaid b y an ab undan t ove rg row th of white ae ria l floccose
hyphae , lending a lanate a ppearance to th e colony su rface ; odo u r stro ng,
aro matic; ex udate lacking ; no colou re d pigment diffu sin g in to th e su rro u ndi ng
agar ; colo ny rev erse (co lour pl ate 162 , c) in yellowish orange shades.
T he vari cty descrip tion is ccntcrcd upon the typc strn in o us :H:1. 5 1 - C M I
- ATCC 111 , 2 :~ 2 - Q M ll, 1411 - IJFM 7 15G, isola tcd by Mrs S. Marcus fr orn
Musa sapicntum [ruit , a l th e London Schoo l 01" H ygien c a nd T ropical M edi cine ,
K, May, 1947.
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Penicillium verrucosum Dierckx varo melanochlorum Samson, Stolk et Hadlok Studies in Mycology . 11: 41-42.1976 .
Synonymy
Colonies u pon CA (colour plat e 163, A and a) att ain ing a diameter of about
40 m m in 14 days at roo m temperat u re (20 -25 OC ), consisting 01" a den se
vclvcty laye r 01' m on ou cruatou s con idiopho res. soru etimes showing so rne lascieulate gro wth in the margin al arcas, ran gin g from ab out 300 to 500 .un dccp in
marginal to submarg inal zon es, and up lo 1- 2 mm in the colony cent re, with
su rfaee appearing granu lar 01' meal y but gen erall y not eo nsp ieuo usly
synnem at ou s, 01't en narrowl y zo nate with shallow radi al furrows, with centre
mostly eovered by a wh ite floeeose eushion 01' ae rial veget a tive hyphae partly
obseu ring th e othe rw ise heavily spo ring eonidial areas in dark gree n eolours ,
near Ame rican 01' Russian gree n (Sg uy, PI. X XX , 441 , 446) ; odo ur stro ng,
sug gesting ea m phor aroma; ex udate limited in amo u nt, as eolou rless droplets
enmes he d in the myeelial felt ; very pale am be r-co loured pigm ent diffusing into
th e su rro u nd ing agar ; eolony reverse (colou r pla te 163 , a) in pale eream shades
to eolourless; eonidial stru etu res esse ntially sim ilar to the othe r variet ies (fig .
163, A , B a nd C) .
Coloni es upon C YA gro wing more rapidly, attaining a diam eter 01' 60 mm
in 14 days at roo m tempera ture (20-25 oC) (eolour plate 163, B a nd b) ,
appearing granular, with a th in margin su rro u nde d by a frin ge 01' sub me rged
hyphae extending about 3 mm beyond th e ae rial gro wth , with eolony su rfacc
almos t entirely eovered by a eus hio n 01' whitish floeeose ae rial hyphae greatly
obse uring th e othe rw ise abu ndantly spo ring areas in dark blu e-gr een shade s
(Sguy, PI. X X X, 442), sulcated by shallow radial fur row s; odo ur faint,
sug gesting pot atoes; exu date limi ted , as eolourless dropl et s enmeshed in the
myeelial felt; no eoloured pi gm ent diffusin g into th e surrounding agar; colony
reverse (eolour plat e 163, b) developing reddish brown to einnamo n shade s.
Co lonies upon M EA (eolour piate 163, C and e) attain ing a di ameter 01'
50 mm in 14 days at roo m tem per ature (20- 25 OC), eons isting 01' a looser
textured basal felt , eom paratively thin , heavily spo ring, plan e, exee pt 1'01' a
slight floeeul ent overgrowth all over the eolony su rfaee, with very thin margin
surrou nd ed by a frin ge 01' sub me rge d hyphae ex te nding abo ut 4 mm beyond
th e aerial gro wth; eonidial areas in deep blu e-green shades (Sguy, PI. X XX ,
442); odo u r pen etrating, cam pho r-like ; exudate not produeed ; no eolou red
pigm ent di ffusin g into th e su rrou nd ing agar; eolony re verse (eolou r piate
163, e) developing yellowish shades.
The va riety de seription is eentered upon th e typ e culture CES 487 .75,
isolated in 1975 by R . H adl ok from meat produets, Gi essen , Fed eral R epublie
01' G ermany .
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Synonymies
Penicillium ochraceum Thom - 'The Penicillia', p. 309. 1930. (cited as P.
ochraceum (BainierrThom) . Also Raper and Thom, 'A Manual of the Penicillia',
pp . 477-478. 1949.
Penicillium ochraceum Thom varo macrosporum Thom - 'The Penicillia', p, 310.
1930.
Penicillium carneolutescens Smith - Trans. Brit. Mycol. Soco 22: 253. 1939. See
also Raper and Thom, 'A Manual of the Penicillia', pp. 479-481. 1949.
hyphac cx rc nd ing a bo u t :> 111111 be yond (he aeri ul gro w th; gro wing m argin
whitc to du ll bulf in colou r, conidia l arcas !>ecollling yellowish olivo l o dar k
greenish olive 01' buffy olive wh en mature in reccnt isolat es (Sguy, PI. XXIII ,
338, 33 7); odour pron ounced , pen etratin g , 'earthy; pale ycllow- coloured
pigm ent diffusing into th e surroundin g agar ; colony rcversc (colour plat o
164 , a) in yellow 01' vinaceous shades ; co n id ia l struc turcs (li g . 164 , A , B and
C ) as in th e othe r vari eti es.
C oloni es upon C YA (colour plat e 164 , B an d b) a tta ining a di ameter 01'
50 mm in 14 days at room temperature (20 - 25 OC), cons isting 01' a tough , wet
basal felt , cove re d b y a white floccose ove rgrowth 01' ae rial hyph ae and rop es 01'
h yphae , very poorl y spo ring; exudate a nd odo u r lacking; yellow coloured
pi gm ent d iffu sing int o th e surrounding ag ar ; colony rever se (co lou r plat e
164 , b) d eveloping yellow ish to reddish brown shade s .
C oloni es upon M EA (colour piate 164 , C a nd c) attain ing a d ia me te r 01'
45 mm in 14 days at ro om temperature (20 -25 OC), ge ne ra lly plane exce p t
slightly raised in th e cen tre, loose textured with a tcnden cy to become floccose ,
more 0 1' less za nate in margin al area s, co m mo nly becoming definitely
synnematous in age, spo rula ting a bundantly in recent isolat es , with
colouratio n in lighter shade s th an upon C A 0 1' C YA; in olde r strains poorly
spo ring and sho wing ab u ndan t ropiness (co lour piate 164 , C ); odo u r m ou ldy ;
exu date not produced ; no colou re d pi gm ent diffusing into th e su r ro u nd ing
agar; colon y reverse (colou r plat e 164 , e) colou rless .
The variety descr ipti on is ce ntere d upon th e typ e strain CBS 246. 32 = C M !
92, 264 = N R R L 3, 971, originally isolat ed b y G . Bainier , and up on R ap er and
Thom ' s notes (1949) that ade q uately present th e species whe n recen tly
isolat ed . The ty pe strain , al'ter so m any years under con tin uo us la boratory
cultiva tion ha s lost m any 01' its typi cal cultu ra l characteristics and ha s beco me
more pred ominantly funi cu lose and poorl y spo ring .
Stolk el H adlok: fig. 165, colour plat e 165 , and SEM plate 28,
(centre : ri ght) .
Pencillum verrucosum Dierckx varo corymbferum (Westling) Samson, Sto lk et
Hadlok - Studies in Mycology. 11: 36-37. 1976.
Synonymies
Pencillum hrsu tum Dierckx - Ann. Soco Sci. Bruxelles. 25: 89. 1901 .
Pencllum corymbferum We stl ing - Ark . Bot. 11: 92. 1911 . See also Raper and
Thom, ' A Manual of the Penicillia', pp. 540-544. 1949.
C olonies upon C A (co lo u r plat e 165, A and a) gro wing rather rapidl y ,
.\
::.
.
10jJm
..
r-
'b0~
!~ @
'
.
.'
~( i ,
Q{.
'The colony is mealy, granulose or fasciculate on Czapek's agar, the conidiabearing layer being blue-green or yellow-green. The margin of the colony is white ,
1-2 mm wide. The colouration of the reverse side is yellow-orange, yellow-brown
to orange -brown and the pigment does not penetrate the agar . The exudate clear,
occurring in abundance. The conidiophores measure 50-400 Jlm in length, 3-3.6
Jlm in width, with a' granular or roughened surface. Sometimes the surface is
smooth. Branches of 11-24 and 3.4-5 Jlm. The height of the penicillus is 35-50 Jlm
(or more), that of the small penicillus (metulae + phialides) 17-24 Jlm. The phialides
are 7-11 .5 by 2.2-3 Jlm in size. Conidia globose with a thick wall, roughened or .
echinulate, 3.5-5 Jlm in diameter.
The original strain was obtained from Ottawa, Canada as a contaminant of an
agar medium (Raper and Thom 1949). In Abe's strain (1956) the origin is not given .
O ur not es follow :
Colo nies upon CA (colo ur pl at e 166 , A a nd a) a tta ining a d ia me te r of
45 m m in 14 days at room tem perature (20 - 25 OC ), consistin g of a basal felt
com posed of mostly monon em atou s co nidiopho res in a den se stand, with
loosely synnem a tou s conid iopho res in marginal areas, lending a gran ular
800 0
0000
0 0
10jJm
ooo~ o\
O
L...--....J
100 jJm
Manual of the Penicillia' , pp. 512-516. 1949. See also Samson et al. Studies in
Mycology 11: 20-22. 1976.
at.
Synonymies
?Penicillium glaucum Link - Mag. Ges. Nat. Freunde Berl. 3: 17. 1809; ex Pers. Mycol. Europ. 1: 40. 1822.
Penicillium elongatum Dierckx - Ann. Soc o Sci. Bruxelles 25: 87. 1901.
Penicillium juglandis Weidemann - Z. Bakt. Parasitenk. Abt. 2, 19: 683. 1907.
Penicillium variabile Wehmer - Mycol. Z. 2: 195. 1913, and Ber. Bot . Ges. 31:
310. 1913. (Not Penicillium variabile Sopp - Videnskapselskapets Skrifter
Kristiania Math. Nat. KI. 11: 171. 1912).
Penicillium plumiferum Demelius - Verh. Zool. -Bot. Ges. Wien. 72: 76. 1923.
Penicillium malivorum Ciferri - Riv. Patol. Veg. Padova. 14: 77. 1924.
Penicillium resticulosum Birkinshaw, Raistrick et Smith - Biochem. J . 36: 829.
1942.
C olonics upon C A (colour plal c 16B , A and a) gro wing rapidly, a uaining a
di ameter 01' abo ut 50 mm in 14 days at room temperature (20 - 25 OC), with
surface plane 0 1' gene ra lly sho wi ng rad ial fur rows , 01'ten ranging frorn 0.5 to
~. O mm deep , with a thin , irregul arl y di ssected, white m argin surrounded by a
tringe 01' su b me rge d hyphae ex tend ing about 2- 3 mm beyond th e ae rial
g-rowth ; ve ry heavily sporing through out with con id iophores very abundant
a nd primarily a rising from th e su bs tratu m, in sorne strains in an alm ost
continuous dense sta nd, in others (colour piate 168, A) sho wi ng conid iopho res
almos t enti rely gro u pe d into fasc icles 0 1' synne mata with colony su rface
ap pearing gran ula r 0 1' showing conspicuo us zonatio n; conid ial areas in yellowgree n to sage gr een sha des (Sguy, Pis XXVII, 403, a nd XXVIII , 417) with
the ripening 01' co nid ia; exudate limited in a mo u nt, mostly as small dropl et s
cnrneshe d in th e m ycelial felt , colou rle ss; odo u r strong, mouldy , characteristic
01' rott in g a pples; reverse almost colou rless in sorne stra ins throu gh yellow brown to red di sh ora nge in othe rs (colour plat e 168, a); am be r-coloured
pigm ent diffusing in to the surround ing agar produced in sorne strains, not in
ot hers ; conid ial structures ve ry abundant an d , in m a ss, characterizing th e
colony , with con id iop hores m ostly 150-400 p.m in length in sorne strains, but
commonly 600 -700 p.m in othe rs, by 3-4 tut: in di ameter with wa lls smooth 0 1'
de lica tely rou gh ened , term inati ng in large penicilli usu ally measuring u p to
75- 100 uu: in length , typica lly asymmetrica l, two - 0 1' three-stage branch ed ,
with rami m ostl y 20-25 uit: by 2.5 -4 .5 tui , occasionally up to 60 p.m in
length , typi call y appresse d agains t th e main ax is; m etulae bo rne in verticils 01'
2-6 eleme nts, abo ut 10- 15 p.m by 2.5-3.5 p.m ; phialides bo ttle shape d, in
cluste rs 01' 2- 8 ele me nts in th e verticil, ranging fro m 8 to 12 p.m by
2.3-3 .0 tui , tapering grad ually to a conspicuo us short and narrowed nec k;
conid ia ellipt ical whe n first formed , th en becom ing su bg lobose, 3- 4 p.m in
long ax is, with walls smooth 0 1' ne arly so (fig. 168, and SE M plat e 28, bott om :
right).
Colonies upon CYA (colour pl ate 168, B and b) attaining a d iame te r 01'
abo ut 65 m m in 14 days at roo m temperatu re (20-2 5 OC ), sim ila rly textu red
as upon C A but m or e loosely synne matous and gra n ula r , rad iately wrinkled
and heavily spo ring throu gh ou t , especially in m argin al areas, in sage green
colours (Sguy, PI. XXVII , 403), su bmarginal a nd central areas with conid ial
layer obscu red by a floccose overgrowth 01' whi te ae rial hyph ae ; odo u r stro ng,
as aboye ; exu date limited in amo u nt, colou rless, 0 1' lacking ; am ber-colou re d
pigm ent di ffusin g in sorne strains, not in ot he rs ; colon y reverse developing
deep reddish orange shades (co lour plate 168, b) ; penicilli esse ntially as
described aboye .
Colon ies u pon MEA (colour plat e 168, C a nd e) attain ing a d iameter 01'
abo ut 65 m m in 14 days (20 -25 OC), cons ist ing 01' a loose- textured ba sal felt ,
pla ne, not furro wed , conspic uo us ly zonat e , with very th in margi n su rro u nded
by a wid e Irin gc 0 1' su b me rgcd hyphac cx tc nd ing about 7 01' more mm bcyond
th e a crial g ro w th ; heavily spo r ing throu ghout in gray-green shades (S guy , PI.
XXIX, 429), with conid iophores a rising dircctly Iro rn th c substraturn in
definite fascicles a rrange d in conspicuo us conce ntrical zon es; ex uda te lacking;
odou r strong , as not ed abo ye; no coloured pigm ent diffusin g int o th e
surro und ing agar; colony rev erse (colou r plat e 168 , e) colou rle ss; penicilli
esse ntially as described aboye .
The spec ies description is ce ntere d upon th e neotyp e stra in CBS 325. 48 = CMI
39,76 1 = N R R L 976 = ATCC 7,861 = IJ FM 5 14 1, isolated frorn apples by j .S.
Cooley , Bureau 01' Plant Industry , US Dep . Agri culture , 1926 , and m any
othe r strains isolated by the a ut ho r fro rn rotten apples in M adrid .
PeniciLlium expansum is one 01' th e oldes t and best known spec ies 01' PeniciLlium,
cons titutin g, with P. glaucum Link and P. candidum Link, th e three spec ies upon
whi ch H .F. Link esta blishe d in 1809 th e genus PeniciLlium.
Hundreds 01' stra ins bel ongin g to thi s spec ies hav e been isolated from
naturally infected products. They a re com mo n in or on organ ic m at erial s in
contac t with th e soil; they occasi on ally occ u r on grains and ce real products,
including bread . They have been isolated from eggs and m eat products in cold
storage, a nd fro m th e waste su lph ite liquors 01' th e wood pulp industr y . They
are occasionally isolat ed from fabrics and othe r deteri or ating military
equ ipme n t. They are, however, m ost com mo n a nd m ost characte ristica lly
isolat ed fro m a pples and othe r pom aceou s fru its, wh er e th ey typi cally produce
a rapid soft brown ro t, hi ghl y destru cti ve , com mo nly referred to as ' blue moul d ' rol. The spo res 01' th e fu ngus a re ca rried from th e soil on th e surface 01'
th e fruit , germ inating and ente ring throu gh cuts and abra sions in th e cuticle
a nd epide rm is .
When tested in th e laborat ory by piercing a nd inocul at in g sound apples,
typ ical strains 01' PeniciLlium expansum will produce areas 01' rot from 30 to
40 mm in di amet er , exte nd ing inward to th e core, wi thin a peri od 01' 8 - 10
days.
Synonymies
Penicil/ium italicum Wehmer - Hedwigia. 33: 211. 1894. See also Beitr. Kennt .
Einh. Pilze, Abt. 2, pp . 68-72. 1895. Also Thom, 'The Penicillia', pp. 412-414.
1930. Also Raper and Thom, 'A Manual of the Penicillia', pp. 526-529. 1949.
Penicil/ium aeruginosum Dierckx - Ann. Soco Sci. Bruxelles. 25: 87. 1901.
Penicil/ium ventruosum Westling - Ark. Bot. 11: 112. 1911.
'1-/:1
lJJ.illlluJ
10J.lm
C)
O O
0
0
B
O
Ot:0
OOCJO
0
00
C)
y~?t~
lOo~Jl
't 11>
the surface 01' th e agar bc fore assuming un cree ! or ascc nd ing position (colour
plate 169, A) ; peni cil1i asymmetric (fig . 169, A), with con id iophores sm ooth
wal1ed , two-stage branched , usu ally with rami closcly a ppressed; stipes
ranging from 100 to 250 flm by 3.5 -5. 0 flm ; ra m i usually m ca suring
15-2 5 flm by 3.5-4.5 flm ; m etulae mor e or less cylind rical, borne singly or in
grou ps 01' 2-4 cle me nts in th e ve rticil, abo u t 15-20 tu by 3- 4.5 tui , bu t
frequentI y larger or smaller , sometime s ari sing a t different levels in the
peni cillu s; phialides slende r, mor e or less cylind rical, with short but
cons picuo us neck , com mo nly 10-1 3 flm by 4 flm and not infrequentI y up to 20
or m ore flm in length; con id ia typi call y cylind rical wh en young but be coming
definitely ellipt ical or eve n su bglobo se at m aturity , ex tre mely va riable, but
generally 5-6 flm by 3.0 -3.5 tun , with indi vidual spo res wh en elliptical
ranging u p to 9 flm by 5. 0- 6.5 tui , with wall s smooth or nearly so (fig . 169, B,
and SE M plate 29, top: left ) ; conid ial chains ta ngled (fig . 169, C ).
Colon ies upon C YA (colour plate 169, B and b) spre adi ng bro adl y , a ttaining
a di amet er 01' abo ut 65 m m in 14 days at roo m temper ature (20-25 OC ),
consis ting 01' a close-t extured b asal felt , cons picuo us ly za nate a nd heavily
spo ring , with conidio pho res aggregated in to well-de fined synne mata arrange d
in conce ntric zones; coni d ial areas in dee p gree n shades (Sg uy, PI. X X IX,
422); exudate limi ted , colourless; od our pro no unce d, ' mo uldy'; am be rcoloured pi gment d iffus ing int o th e su rrou nding agar; colony reverse (colour
pIate 169, b ) cons picuo us ly zonate, develop in g dark b rown zones alte rnating
with reddish bro wn arcas; penicilli esse ntially as desc ribe d above .
Colon ies upon MEA (colou r plat e 169, C a nd c) attain ing a diamet er 01'
abo u t 55 mm in 14 days at roo m tempera ture (20 -25 OC), cons ist ing 01' a
loose-textured basal feIt , cons picuou sly za nate ow ing to the fascicul at e conid ioph or es , with a very th in m argin su rro unde d by a wide fringe 01' submerged
hyphae exte nd ing abo ut 5 mm beyond th e aerial gro wth; heavily spo ring
th ro ughout in th e sa me green shades as above; odo ur strong, aro matic,
suggesting perfume; exudate not produce d; n o colou re d pi gm en t diffusin g into
th e surrounding agar; colony reverse (colour plate 169, e) cons picuous ly
zonate, developing da rk b rown colou rs; pen icilli esse nt ially as described
above.
The spec ies description is ce ntered upon th e neot yp e strain C BS 339.48 = C M I
39,760 = ATCC 10,454 = NRRL 983, isolated by Dr H .S . Fawcett, Ci trus
Experiment Station, Ri versid e , California, fro m citr us fruits, in 1930, a nd
m an y othe r strains isolat ed fro m citru s frui ts in Spain, by th e aut ho r. (colour
pIate 247 presents a representat ive strain 01' P. italicum var. italicum isolat ed by
th e autho r fro m Cayenne pe pper.)
Penicillium italicum va r. italicum is rarely found in n ature except on citr us
fruits, wh ere it ca uses a characteristic soft rot, appeari ng in advanced stages 01'
fru it dec ay as a bluish green m ould cove ring th e rind as a velvety a l' tu fted
~ 77
lnycr 01' powdcry spores. The rol ca use d by thi s spccics is clearly distin ct Irom
rluu ca use d by P. digitatum Sa ccardo . Both sp ecies m ay occ ur o n the sa me
individual fru it. The char ac te r ist ic so ft rot is generall y referred lo as ' b luemould ' citrus rot. P. italicum val'. italicum ca n be distinguish ed from P. digitatum
by th e stru cture a n d colo ur o f their co lo n ies, those of th e former are
synnem at ou s a n d blue- green , while th ose of the latter a re thin , velvety a n d
olivaceou s . M oreover , P. italicum gro ws better upon C zapek ' s agar th an P.
dZttitatum .
170. Penicillium italicum W ehmer v a l'. avellaneum Samson et G u tter: fig .
-!{~\
,..\;1j
fR .
~
100j.lm
11"{11
some timo s obscurcd by whit c locc ose acri al hyphae es pec ia lly in th c colon y
ce ntre; co n id ia l a rcas pale brown, near cinnarnon, becorning d arker, nca r
ave llane ous (Sgu y , PI. IX, 134) ; odour aromatic; exu d ate not produ ccd ;
conid iopho res mon on ematous 01' syn ne mato us, with synnemat a especially
present in m arginal areas, ofte n d evel oping from bundles of submerged
hyphac ; co ni d iophores smoo th walled , at least in th e upper parts, two-stage
b ranched (fig . 170, A), with rami usually elos ely a ppressed; with penicilli
duplicat in g in det ails of mi cr oscopi cal morphology , in size and shape those of
th e va l'. italicum; con id ia cylind rica l, sometimes ellipso idal, smooth walled (fig,
170, B, a nd SEM plat e 29, top : ri ght) , pal e brown in m ass , 4. 5. ,tm by
2.8- 4.0 ustv ; no coloured pi gmcnt diffu sing into th e su rrou nd ing agar; colon y
rever se ve ry dark violet, almost bl ack colou red (colour plat e 170, a); conid ial
chains loo sely tangled (fig . 170, C).
C olonics upon C YA (colour plat e 170, B and b), sp read ing broadly,
att aining a diamet er of abo u t 65 - 70 mm in 14 d ays at room temperature
(20- 25 OC), co nsisting of a dense basal felt , plane in margin al areas and
stro ng ly furrowed in th e cen tre in a radial pattern , with a thin margin
surrou nde d by a fringe of su b merge d hyphae ex te nd ing a bo u t 3 mm beyond
th e ae rial growth ; heavily spo ring th rou ghout in avella neous shades, show in g
sorn e zonat ion owing to th e syn ne matous co nid iopho res arranged in co ncen trical cireles; odo u r aro matic; ex udate not produced ; am ber-colo u re d
pi gm ent diffu sin g into th e surro unding agar; colony reverse (colour pl ate
170, b) in de ep reddish brown shades; penicilli essentially as described abo ye
for th e val'. italicum .
Colonies upon M EA (co lo u r plat e 170, C a nd c) attaining a di amet er of
abo u t 55 mm in 14 d ays at room tempera ture (20 -25 OC), co ns ist ing of a
looser textured basal felt , a p peari ng velvety to ' mcaly', plane , not furrowed ,
very sligh tly umbon ate in th e cen tre, with very thin m argin su r ro u nde d by a
frin ge of su b me rge d hyphae ex te nd ing abo u t 3- 4 mm beyond th e ae ria l
gr owth ; heavil y spo ring th rough out in th e sa me shades as a boye ; ex udate not
produ ced ; odo u r aro matic; no coloured pi gm ent d iffus ing in to th e surrou ding
agar; colon y rever se (colour pla te 170, c) d evel opin g yellow ish shades;
con id ioge no us structures identical with th ose on C A and CYA .
T he va riety description is ce n te re d upon the type stra in CBS 719. 73 = IJ FM
7193, isolated from Citrus sp. in Bet D a gan , Israel , by Y . G u tte r in 1973.
The varie ty diffcrs fro m th e val'. italicum m ainly b y th e avella neou s colour.
V al'. avellaneum ca n be poten tially a serious post-harvest ca use of infecti on
and su bse q ue n t decay of citrus fru its.
Colonies upon C A (colour pIate 171, A and a) gro wing rap idly , attaini ng a
d iameter 01' abou t 45 mm in 14 days a t roo m temperature (20- 25 OC ) ,
consisting 01' a dense , stro ngly syn ne matous, felt , with synne mata a rrange d in
a circula r pattern, with colony surface appearing coarsely gran ula r and with
th e great maj ority 01' conid iopho res aggregated into c1early defin ed fascic1es,
1- 2 mm deep in central and subcentral areas, not furrowed , with thin m argin
su rrou nde d by a fringe 01' su bmerged h yphae extending abo ut 3- 4 m m beyond
the ae rial gro wth; hea vily sporing throu gh out in deep blue-gr een shades
(Sguy, PI. XXX , 447) with th e rip ening 01' con id ia; exudate lacking ; odo ur
fragra nt, aro m atic; pale yellow-coloure d pigm ent slowly di ffusin g in to th e
surrou nd ing agar; colony reverse (colour plat e 171, a) developing yellowish
sha des with a green cas t sho wing throu gh ; penicilli (fig . 171, A) typi call y
as ym me tr ic, with conid iophore s coarsely rough en ed , inc1udin g the first rami ,
the ot he r ele me nts a re smooth wa lled, usu all y two- , three- 01' mo re stage
:.~. !
o
8 0
0 0 00
0 0 0
O 00
'"
r
/......
."
~ :/
"
s, ;;
j"'
:.:
.'
~:
Ir
",
....
fR.,
e,
branched , with all eleme nts multisep tat e so as to m ake its differ entiati on difficult ; all eleme nts of th e pen icillu s are closely appressed; stipes of th e
conidiophores va ry ing in length , rangin g from 50 to 200 p.m in len gth by
4.0 -4 .5 uit: in di ameter; b ranc hes or ram i va ria ble in form and size, usu ally
becoming small er at successive levels away fro m th e m ain ax is; me tulae not
readily di stingui shabl e from th e u pp ermost rami , m ostly 4.0-11 .0 p.m by
3.0 - 5.0 p.m ; stipes and first rami appearing cons picuo us ly warty , with
rem aining conid ial struc tu res smooth walled ; phialides bottle shape d,
com paratively sm all , mostl y 6.0-8 .0 p.m by 2.5 -3 .0 psi , with a conspicuo us
bu t sho rt neck ; conid ia globose to su bg lobose, 3.0- 4.0 p.m by 2.5- 3. 5 p.m,
smooth wall ed or nearl y so (fig. 171, B) .
Colonies upon C YA (colour plate 171 , B a nd b) attaining a di amet er of
55 mm in 14 days at room temperature (20- 25 OC ) , sim ila r in texture and
colony appearance to those gr own upon C A, but showing more abu ndant
fascicul a tion , with colony su rface radi at ely furro wed and za nate in m argin al
areas; h eavil y spo ring th roughout in blue-gr een sha des (Sguy , PI. XXX ,
447 ) ; exudate and odo ur lacking ; pale amber-coloured pigment diffusin g in to
the surrou nd ing aga r ; colo ny reverso (colour plale 171, b) developin g yellowi sh
cin narnon shadcs; peni cilli essc n tially as cl cscribcd aboye .
Colonies upon M EA (co lou r plat e 171, C and e) a tta in ing a diamet er of
55 mm in 14 d ays at room temperature (20-25 OC ), cons istin g of a loosetextured ba sal felt , co ns picuo usly zonate ow ing to th e co nce n trica lly a rra nged
syn ne mata; heavil y spo ring throughout in deep blue-gr een shades (Sguy , PI.
XXX, 446); ex udate lacking ; odo ur fragrant , fruity ; no colo ured pigment
diffusing into th e surrounding a gar ; colony reverse colou rless with a yellow
cast showing th rou gh (colour plate 171, e) . Penicilli essen tially a s described
a boye.
The Penicillium hispalense description is centered upon the typ e strain IJ FM
5940, isolat ed in ou r laboratory as a cult ure con taminan t in 1978. It is ac tually
kn own only fro m th e typ e strain.
Penicillium hispalense is a di stincti ve species characte rize d m ainly by its
see m ingly polyv erticillate penicilli , owing to th e multiseptate characte r of th e
e1ements of its penicilli , and by its strong ly synnematou s colo n ies .
The correc t pl acement of thi s species is sti ll ope n to questi on since it ap pears
as an intermedi at e between th e Asym m etric- fasc icu la ta and th e Polyverti cillat a
sect ions (Raper a nd Thom 1949). H ow ever , for th e time being , it is tentati vely
assigne d to the for me r sectio n, near P. granulatum Bainier.
Synonymies
Penicillium patu/um Bainier - Bull. Soco Mycol. Fr. 22: 208. 1906.
Penicillium urticae Bainier - Bull. Soco Mycol. Fr. 23: 15. 1907.
Penicillium f/exuosum Dale - Ann. Mycol. 24: 137. 1926.
Penicillium ma/tum Hori et Yamamoto - Jpn. J. Bacteriol. 9: 1105. 1954 (not
validly published) .
o
00
00 00
0 0 000 O
O 0 0 00
o O 8
O O
00 O
0 0
O
l.w..lL.w.J
jrrn
10
'IIU
4114
H owever, since patulin is app rcciab ly toxic to a nimals (Abraham and Florcy,
1949), it is regarded ac tually as a mycotoxi n .
173 . Penicillium granulatum Ba ini er : fig. 173 , colour plat e 173 , a nd SEM
$V
10 um
00 O
O OO ~OC)()
00 0 0 C)
00 O
'----'
100,JJm
Penicil/ium granulatum Bainier - Bull . Soc o Mycol. Fr. 21: 126. 1905.
Synonymies
Penicil/ium divergens Bainier et Sartory- Bull. Soco Mycol. Fr. 28: 270. 1912.
Penicil/ium schneggii Boas - Mycol. Z. 5: 73-83. 1914; and Z. Bakt. Parasitenk .
Abt. 2, 44: 695-696. 1916.
Co loni es u pon C A (colour pl at e 173, A a nd a) attaining a di ameter of abou t
40 mm in 14 days at room temperature (20 -25 OC), com pa ratively deep , up to
2-4 mm , m ore 01' less floccu lent wit h abu ndant sterile hyphae white to dull
'111:>
yc llow co lo urcd , with surlacc irrcgularly tuft cd ami radi at cl y wrinklcd , with a
I hin m argin su r ro u nde d by a narrow Iringc 01' su b mer ged h yphae exte nd ing
nhou t 1 mm beyond th e aerial growth; m edium sporing in pale blue-green
shades (Sguy , PI. XXIX , 428) ; ex u date limited to abundantIy produced ,
clca r lo pal e yell ow ; odour a romatic, usu ally pronounced ; colony reverse
usually in dull ye llow to orange 01' reddish brown shades (colour pia te 173, a),
wirh a gr een cas t showing through in marginal areas ; penicilli as ym m etric (fig.
173, A) , comparatively large, with con id iop hores sometimes m ononematous,
but more com m on ly synnematous to loosely synnematous a nd in zones ,
cspecially in m arginal areas (fig . 173, D), consp icu ou sly rough-walled , two- to
ihree-stag e branched; stipes variable in length, from 100 to 200 utt: up to very
long in synnemata, about 3.5- 4 .0 p.m in diameter; rami variable, com m only
12-30 p.m by 3.0 - 3.5 iui, somewhat appressed against the main axis;
metulae m ore 01' less cylindrical, usually rough wall ed , few in the verticil,
about 8-12 p.m by 3.0- 3.5 p.m ; phialides bottIe shape d , with a short but
consp icu ou s neck, in small crowde d clusters , usually about 7 -10 p.m b y
3.0-3 .5 p.m; conidia strongly elliptical when you n g, in age elliptical to
subglobose, 4-5 utt: by 3-4 p.m in di ameter, with walls smooth 01' nearly so
(fig . 173, B, and SEM plate 29, ce n tre: right) ; conidial ch a in s tangled 01'
divergent (fig . 173, C).
Colonies upon CYA (colour plate 173, B and b) a tta in ing a diameter 01'
45 mm in 14 days at room temperature (20-25 OC ), similar in texture as
described ab oye but poorly spor ing in brownish shades, with basal felt ov erlaid
by a dense overgrowth 01' whitish ste r ile aerial hyphae ; with a thin margin
surrounded by a fringe 01' submerged h yphae ext ending a bo u t 2- 3 mm b eyond
th e ae r ial growth ; colony surface strongly radiat ely furrowed a nd irregul arly
fold ed in th e cen tre; od our strong, suggestin g a m m onia ; exu date n ot
produced ; no colou re d pigment .diffusing into th e su rrou nd in g aga r ; co lony
rever se (colour plate 173, b) in reddish orange sh ad es ; penicilli esse n tially as
described aboye .
Colon ies upon MEA (colou r pl at e 173, C a n d e) gro wing variously , in sorne
stra ins a ttain in g a diamet er 01' 40-50 mm in 14 d ays a t room temperature
(20-25 OC ), in others restrict ed a n d not exceed ing 25 mm in th e same period
(colou r plate 173, A) , plane , not furrowed , with su rface consp icuo us ly
gr anular from the production 01' abundant fas cicles up to 1 mm 01' more in
length; heavily sporing throughout, in dull gra y-green shades (Sguy,
PI. XXIX , 426) , with surface partIy ea ve re d by an overgrowth 0 1' white
floccose ae r ia l h yphae , obsc u rin g more 01' less th e fru iting areas; exu date not
produced ; odour arom ati c; penicilli essen tially as d escribed aboye ; n o colou re d
pi gm ent diffusing into th e su rrou nd ing agar; colon y rev erse (co lou r pia te
173, e) in yellowish orange shade s.
The specie s description is centered upon th e n eo typ e strain C BS