MÉTODO BRADFORD

[BSA] microg ABS 595 nm media

P1 25 1.749
P1' 25 1.724 1.7365
P2 18 1.579
P2' 18 1.504 1.5415
P3 12.5 1.04
P3' 12.5 1.338 1.189 el 1,034 no lo usamos se va mucho
P4 6.25 1.034
P4' 6.25 1.208 1.121
P5 0 0.742
P5' 0 0.706 0.724

M 1.134
M' 1.227

El blanco es el aire

Chart Title
2
1.8
f(x) = 0.0395499083049515 x + 0.802158632433849
1.6 R² = 0.97377981486333
1.4
1.2
[BSA] microg ABS 595 nm 1
25 1.7365 0.8
18 1.5415 0.6
12.5 1.33 0.4
6.25 1.121 0.2
0 0.724 0
0 5 10 15 20 25 30

abs []dil []microg/mL media

M 1.134 8.4 10500
M' 1.227 10.7544304 13443.037974684 11971.519

PREPARAR EPP PARA CARGAR ELECTROFRESIS CON DISTINTAS []

 microg Cf microg/mLVf microL Vi microL de mi M V relleno H2O
1 7.5 250 30 0.7 23.3
2 15 500 30 1.5 22.5 6 TC
3 30 1000 30 2.9 21.1
4 45 1500 30 4.4 19.6

La concentración de la que parto es 10320.275

microg/mL
20 25 30
resultados alina
abs
25 1.775
25 1.71 1.7425
18 1.52
18 1.485 1.5025
12.5 1.051
12.5 1.285 1.168
6.25 1.16
6.25 1.093 1.1265
0 0.722
0 0.705 0.7135
Chart Title
2
m 1.314
1.8
m' 1.44 f(x) = 0.0394056195965418 x + 0.787340597982
1.6 R² = 0.975257346133778
1.4
1.2
1
0.8
[] abs
0.6
25 1.7425
0.4
0.2
18 1.5025
0
0 5 10 15
12.5 1.285

6.25 1.1265

0 0.7135

abs [] []final
m 1.314 13.3680203 16710.0254
m' 1.44 16.5659898 20707.4873 18708.7563
 Chart Title

= 0.0394056195965418 x + 0.787340597982709
0.975257346133778

5 10 15 20 25 30