CURVA PATRON DE GLUCOSA

Concentracion (mg/0,5 ml) Abs1 Abs2

3.35 2.464 2.416
2.50 1.964 1.955
1.65 1.305 1.292
1.00 0.771 0.722

Ensayo 1
Diluciones Abs 1 Conc. Azuc. (mg/0.5ml)
D1 2.045 2.719
D2 2.050 2.726
D3 2.044 2.719
D4 2.041 2.714
D5 2.052 2.729

Y X
Conc. Azuc.
Diluciones (mg/0.5ml)/promedio Proporción enzima
D1 2.570 0.00875
D2 2.577 0.00750
D3 2.570 0.00500
D4 2.565 0.00375
D5 2.581 0.00250
 Blanco enzima Abs 1
Abs promedio BD1 -0.007
2.440 BD2 -0.003
1.960 BD3 0.011
1.299 BD4 0.010
0.747 BD5 0.009

Ensayo 2 Ensayo 3
Abs 2 Conc. Azuc. (mg/0.5ml) Abs 3 Conc. Azuc. (mg/0.5ml)
1.839 2.44 1.925 2.55
1.844 2.44 1.930 2.56
1.838 2.44 1.924 2.55
1.835 2.43 1.921 2.55
1.846 2.45 1.932 2.56

Y (Concentración) X (Proporcion de enzima) Grafica

Menor 2.565 0.00375 2.585
Mayor 2.581 0.0025
2.58 f(x) = − 12.8

Concentracion azucares
R² = 1
2.575
y = -12,8x + 2,613
2.57
2,565=-12,8x + 2,613
2,565=-12,8x + 2,613 2.565
x: 2,565-2,613/-12,8 2.56
x: 0,00375
2.555
0.002 0.0022 0.0024 0.0026 0.002
FPU/ml
Propo
99.1957104557641
 Abs 2 Abs promedio
0.040 0.017
0.026 0.012
CURVA PATRON DE GLUCOSA
0.023 0.017
0.031 0.021 4
0.009 0.009 3.5
3 f(x) = 1.37098580067428 x − 0.083829498111348
R² = 0.992279695007577

Concentración
2.5
2
Proporción enzima 1.5
0.00875
1
0.00750
0.5
0.00500
0
0.00375 0.600 0.800 1.000 1.200 1.400 1.600 1.800 2.000 2.200 2.400
0.00250 Absorbancia

Grafica AR vs PE

f(x) = − 12.8 x + 2.613

R² = 1

2 0.0022 0.0024 0.0026 0.0028 0.003 0.0032 0.0034 0.0036 0.0038 0.004
Proporción enzima
DE GLUCOSA

083829498111348

600 1.800 2.000 2.200 2.400 2.600

bancia