Medio de cultivo Desecho liq.

1 Triton-x-100
EDTA
Seccin de recuperacin primaria Desecho liq. 2
S-105
S-104
P-6 / AF-102
S-103 Air Filtration
S-101 S-117
P-2 / ST-101 S-116
S-112
Agua P-1 / V-103 Heat Sterilization S-114 P-11 / HG-101
S-111 S-119
Mixing P-10 / DS-101 P-9 / V-101 Homogenization P-12 / DS-102
P-3 / FR-101 Centrifugation Blending / Storage P-13 / V-102
Centrifugation
S-110 Fermentation P-8 / DCS-101 Blending / Storage
Amonio CNBr/HCOOH Storage WFI-1 Desecho liq. 3 S-121
MrETOH/Urea
S-109 P-4 / AF-101
Aire Air Filtration
P-5 / MX-101 S-128
S-108 Mixing S-130 S-125
S-123
P-14 / DS-103
P-16 / DF-101 P-15 / SR-101
P-7 / G-101 P-17 / DE-101 Centrifugation
Guan HCl IB Solubilization
Gas Compression WFI-2 P18 / R-104 Dead-End Filtration Diafiltration

CNBr Cleavage Desecho liq. 4

S-132
Desecho liq. 5

S-133 Seccin de reaccin

S-136 S-162
S-134 P19 / GBX-102 S-163
P-22 / DF-102 S-151 Enzimas S-164
Rotatory evaporator
P-24 / R-103 S-152
Diafiltration
WFI-3 S-153 WFI-4
Sulfitolysis
-139
Desecho liq. 6
S-154
S-146 S-156
S-143
S-142 S-161
S-141 S-150 S-158
S-144 S-147
S-140 P-27 / DF-104 P-28 / C-103
P-21 / C-102 P-26 / R-105 Diafiltration
P-18 / DF-103 S-Sepharose
HIC column S-102
P-19 / C-101 P-23 / R-102 Diafiltration Enzyme conversion
Desecho liq. 11
S-Sepharose Refolding
Desecho liq. 8 Desecho liq. 9
Desecho liq. 10
MrEtOH
Desecho liq. 7
 Seccin de purificacin final

S-179
S-187 WFI-E
S-182 S-178
S-185 S-177
P-29 / DF-105
Diafiltration S-107
S-115
S-184 S-181
S-176

Desecho liq 13
P-31 / DF-106
S-186 P-33 / DF-107
Diafiltration
Diafiltration P-32 / C-105
S-173
P-20 / BCF-101 Gel Filtration
S-106 P-34 / R-106
Basket Centrifugation Desecho liq. 14 S-169
Crystalization
Desecho liq. 16 Desecho liq. 15 S-170
Desecho liq. 17 S-171

S-190

P-30 / C-104
RP-HPLC

Producto

P-35 / FDR-101 Desecho liq. 13

Freeze Drying

P-37
Truck (Bulk)
Comercializacin del p.