Figura 4.

Punto Isoelctrico de la Insulina

Tubo cido
Acetato
Insulina
pH
No.
Actic de sodio
40U/ml
o 0.2M
0.2M
(mL)
(mL)
1
0.23
0.02
6 U (0.06 mL) 3.6
7
2
0.15
0.10
6 U (0.06 mL)

Observaciones

3
0.5
0.20
6 U (0.06 mL)
4
0.01
0.24
6 U (0.06 mL)
5
0.00
0.25
6 U (0.06 mL)
Alejandro no recuerdo como eran los precipitados

No se observ
precipitado
No se observ
precipitado
+
++
+++

Calculos
Tubo1
Concentracin del acido

( 0.23 mL )

Concentracin de la base

( 0.02 mL )

(A)
pH= pka+ log ( HA )

0.2 M
=( 4.6 x 10
( 1000
mL )

0.2 M
=( 4 x 10
( 1000
mL )

pH=4.74 +log

( 4 x 106 )
5

(4.6 x 10 )

=3.67

Tubo 2
Concentracin del acido

( 0.15 mL )

0.2 M
=( 3 x 10
( 1000
mL )

Concentracin de la base

( 0. 10mL )

0.2 M
=(2 x 10
( 1000
mL )

(A)
pH= pka+ log ( HA )

pH=4.74 +log

( 2 x 105 )
5

(3 x 10 )

=4.56

Tubo 3

0.2 M
=( 1 x 10
( 1000
mL )

Concentracin del acido

( 0.5 mL )

Concentracin de la base

( 0. 20 mL )

0.2 M
=( 4 x 10
( 1000
mL )

(A)
pH= pka+ log ( HA )

pH=4.74 +log

( 4 x 105 )
4

(1 x 10 )

=4.34

Tubo 4
Concentracin del acido

( 0.01 mL )

0.2 M
=(2 x 10
( 1000
mL )

Concentracin de la base

( 0. 24 mL )

0.2 M
=(4 .8 x 10
( 1000
mL )

(A)
pH= pka+ log ( HA )

pH=4.74 +log

( 4.8 x 105 )
(2 x 106 )

=6.12

Tubo 5
Concentracin del acido

( 0.00 mL )

Concentracin de la base

( 0.24 mL )

pOH= 7 + pka (1/2 log (A))

0.2 M
=0
( 1000
mL )

0.2 M
=(4.8 x 10
( 1000
mL )

1
5
pO H=7 +2.37( log 4.8 x 10 )=1.88
2