Curva estndar de Rojo Congo

Concentracin
0
3.125
6.25
12.5
25
50
100

Tubo
Blanco
1
2
3
4
5
6

Absorbancia
0
0.112
0.235
0.433
0.858
1.580
2.78

Absorbancia

Absorbancia
1.8
1.6
1.4
1.2
1
0.8
0.6
0.4
0.2
0

y = 0.0312x + 0.0392
R = 0.9982

Absorbancia
Linear (Absorbancia)

10

20

30

40

50

60

Concentracin

Tiempo
0
5
10
15
20
25
30
35
40
45
55
60
70
80
90
100

Va Intravascular
Vaso A
Absorbancia
Concentracin
0
0
1.397
44.74
0.958
30.67
0.785
25.12
0.670
21.44
0.539
17.24
0.470
15.02
0.401
12.81
0.342
10.92
0.276
8.81
0.192
6.11
0.135
4.29
0.104
3.29
0.062
1.95
0.049
1.53
0.044
1.37

Vaso B
Absorbancia
Concentracin
0
0
0.168
5.35
0.299
9.54
0.369
11.79
0.381
12.17
0.401
12.01
0.407
13.26
0.415
13.26
0.415
12.81
0.375
11.98
0.301
9.61
0.260
8.29
0.210
6.69
0.166
5.28
0.162
5.15
0.112
3.55

Absorvancia Vaso A
1.6
1.4

y = 0.0312x + 0.0013
R = 1

Absorbancia

1.2
1
0.8

Absorvancia Vaso A

0.6

Linear (Absorvancia Vaso A)

0.4
0.2
0
0

10

20

30

40

50

Concentracin

Absorbacia Vaso B
0.45
0.4

y = 0.0317x - 0.0015
R = 0.9952

Absorbancia

0.35
0.3
0.25

Absorbacia Vaso B

0.2

Linear (Absorbacia Vaso B)

0.15
0.1
0.05
0
0

10
Concentracin

15

Vaso A

0
5
10
15
20
25
30
35
40
45
55
60
70
80
90
100

Vaso A
Concentracin
0
44.74
30.67
25.12
21.44
17.24
15.02
12.81
10.92
8.81
6.11
4.29
3.29
1.95
1.53
1.37

Concentracion Vaso A
50
40
30
Cp

Tiempo

20
10
0
0

20

40

-10

60

80

100

120

Tiempo

Vaso B

0
5
10
15
20
25
30
35
40
45
55
60
70
80
90
100

Vaso B
Concentracin
0
5.35
9.54
11.79
12.01
12.17
13.26
13.26
12.81
11.98
9.61
8.29
6.69
5.28
5.15
3.55

Concentracion de Vaso B
16
14
12
10
Cp

Tiempo

8
6
4
2
0
0

20

40

60
Tiempo

80

100

120

Vaso B (Extravascular)

Tiempo In[CpDiferencia]

La determinacin de la ke, se realiza a partir de la

funcin ln Cp versus tiempo comprendido de 40 a 100
Vaso B
Concentracin
2.55
2.48
2.26
2.11
1.90
1.66
1.63
1.26

40
45
55
60
70
80
90
100

In[Cp]
3

3.06
2.67
2.29
2.02
1.69
0.95
0.04

In(CpDiferencia)
y = -0.0936x + 3.6886
R = 0.9483

3.5
3
y = -0.0209x + 3.3919
R = 0.9843

2.5

2.5
In[Cp]

Tiempo

5
10
15
20
25
30
35

2
1.5
1

In[Cp]

0.5

1.5

0
0

10

20

30

40

Tiempo

0.5
0
0

20

40

60

80

100

120

Tiempo

Tiempo

CpExtrapolada

CpObservada

CpDiferencia

5
10
15
20
25
30
35

26.77
24.11
21.72
19.56
17.62
15.87
14.30

5.35
9.54
11.79
12.01
12.17
13.26
13.26

21.42
14.57
9.93
7.55
5.45
2.61
1.04

La ecuacin del modelo de un compartimento para la

administracin extravascular se encuentra dada por
la ecuacin 1.

Vd =
Cp = =
t=
t mx. =

Cp(t mx.)=
62.98(
31.49

In[Cp]

F=1

y = -0.0375x + 3.8303
R = 0.9921

D = 20000

3.5
3

Ka= 0.0936

Cl =Ke(Vd) = 0.0209

(866.27mL) = 18.11mL/

In[Cp]

2.5

Ke= 0.0209

2
1.5

ABC:

1
0.5
0
0

+ 53.325 + 59.5 + 60.45 + 63.575

+ 66.3 + 65.175 + 61.975 + 107.95 + 44.75 + 74.9 + 59.85
+ 52.15 + 43.5 = 850.625
ABC = 850.625

20

40

60

80

100

Tiempo

Ke = 0.0375
t = 18.48

Vaso A (Intravascular)
Tiempo
5
10
15
20
25
30
35
40
45
55
60
70
80
90
100

In(Cp)
3.8
3.42
3.22
3.06
2.84
2.7
2.55
2.39
2.17
1.8
1.45
1.19
0.66
0.42
0.31

Vd =

= 434.12mL

Cl =Ke(Vd) = 0.0375

(434.12mL) = 16.27mL/

ABC:

= 188.525 + 139.475 + 116.4 + 96.7 +

80.65 + 69.575 + 59.32 + 49.325 + 74.6 + 26 + 37.9 +
26.2 + 17.4 + 14.5 = 996.57
ABC = 996.57

120