Modelo de Michaelis-Menten

〖−�〗 _𝑆= (𝑉_𝑚𝑎𝑥 ∙ 𝑆)/(𝐾_𝑀+𝑆)

Lineweaver-Burk
1/ 〖−�〗 _� = (𝐾_𝑀+ 𝑆)

S -r 1/S 1/-r
0.2 1.08 5.0 0.93 1/Vmax = 1,3
0.02 0.55 50.0 1.82
0.01 0.38 100.0 2.63 KM/Vma
0.005 0.2 200.0 5.00
0.002 0.09 500.0 11.11 KM=0,0266

Gráfico de Michaelis-Menten
1.2
1
0.8
-rureia

0.6
0.4
0.2
0
0 0.05 0.1 0.15 0.2 0.25
Curéia

Hanes-Woolf
𝑆/ 〖−�〗 _� = �_�/�_��� +1/�_��
S -r S/-r 1/Vmax = 0,826 kmol/m3s
0.2 1.08 0.185
0.02 0.55 0.036 KM/Vmax=0,02 s
0.01 0.38 0.026
0.005 0.2 0.025 KM=0,0242 kmol/m3
0.002 0.09 0.022

Eadie-Hofstee
〖−�〗 _𝑆= �_��� −𝐾_𝑀 〖−�〗 _𝑆/𝑆

S -r -r/S Vmax = 1,2216 kmol/m3s

0.2 1.08 5.4
0.02 0.55 27.5 KM = 0,024 s
0.01 0.38 38.0
0.005 0.2 40.0 KM = 0,0242 kmol/m3
0.002 0.09 45.0
= (𝐾_𝑀+ 𝑆)/(𝑉_𝑚𝑎𝑥 _ 𝑆) _� = 1/�_��� +𝐾_𝑀/𝑉_𝑚𝑎𝑥 1/𝑆
1/ 〖−�〗

1/Vmax = 1,33 kmol/m3s

KM/Vmax=0,02 s

KM=0,0266 kmol/m3

Gráfico de Lineweaver-Burk
12.00
10.00 f(x) = 0.0207267768x + 0.7530807308
8.00 R² = 0.9992014853
-1/ruréia

6.00
4.00
2.00
0.00
0.0 100.0 200.0 300.0 400.0 500.0 600.0
0.25 -1/Curéia

��� +1/�_��� 𝑆
Gráfico de Hanes-Woolf
0.200
f(x) = 0.8264082924x + 0.0198456136
0.150 R² = 0.9997606121
0.100
-r
 Gráfico de Hanes-Woolf
0.200
f(x) = 0.8264082924x + 0.0198456136
kmol/m3s 0.150 R² = 0.9997606121
S/-r 0.100
0.050
0.000
0 0.05 0.1 0.15 0.2 0.25
S

_𝑀 〖−�〗 _𝑆/𝑆

Gráfico de Eadie-Hofstee
kmol/m3s 1.2
1 f(x) = - 0.024427046x + 1.2216352944
R² = 0.9823850272
0.8
0.6
-r

0.4
0.2
0
0.0 5.0 10.0 15.0 20.0 25.0 30.0 35.0 40.0 45.0 50.0
-r/S
600.0
 0.25

45.0 50.0