Moyecos CINE TICO INK BICLOWm Trowrdrcions CoMPETITIVA Dewees a a fo . -. a Viva ts] . + Compete eee = ste ati 3s, 7 es + i o(-@). ; wr rr) aD =? 7 Fig. 1-2. The versus [5] plot in the presence and inthe absence of a Fixed concent tion of # competitive fibiton Fig. 4 The 1/+ ves 1/[] plot in the prezonce of diferent fixed concentrations of 2 Competitive inhibitor. L kw (8) Ly 1 v- Yous ae Vennk 7 8 8 " o Ken eK Kage See Gd Kom Fig. WAS, Replots of data taken from the reciprocal plot. (2) Shey Versus () Kagy versus8 ) Dixon lot for a competitive inhibitor: 1/e versus fin the presence of iferent fined concerations of subst. If Vg, s known, x horizontal ine as heghe 21 7/Vig Ea be drawn directly. (2) Replot ofthe slopes ofthe Den por Lnwoicvow No CoMPeTiTIvA puss Diveeto Vek Ve Us ®) K, + (SJ . [s] ++ Nencompeive ‘ner tk, 1 Fig. LAG, The » vers [5] plot in the presence of a noncompetitive inhibitor(ia = 8p pet Fig. 17, The 1/» versus 1/1] plot in the peesence of diferent fixed concenttations of a noncompetitive inhibitor iK, Il\ 4 l_/ @) tea DS a i Vows Verna n Vaasa Ke feplts of da taken fom the rere po.) Sips versus I.) ty erep Vee V/ Pa) we I = ( Yuu \ tsh) Fig: 1-49. (4) Dion plot for a noncompetitive inhibitor 1/2 versus [I] inthe presence Et iferent fined concentations of substrate. (b) Sipe ceptTwice ACOMPETITIVA SD veecfe. 25¥ina, I fig, 22. The © versus [5] plot inthe presence of an uncompetitive inhibitor rt i iz fig. 23. The 1/ versus 1/[5} plot in the presence of different fixed concentrations of an uncompetitive inhibitor A Ke 1, Ay, Ver walt? ) cet ope (y) Voce Fea i can om ow Pigg) SUS (8) Ravers POM BOL) 7Pixow Seay nr Fig. 1125. Dison plot for an uncompetitive inhibitor: 1/+ versus lat dillerent fixed concentrations ofS Tvmigicvow = MUTA Li GAL Hea i 5 ( ee) ; Ag KK ist dK ig. V4,_The » vesus [5] plot inthe presence ofa lineat mixediype inhibitor where an2and p=0,Doble _yerpwee CL-#) ig. S._The 1/= vers 1/[5] plo in the presence of diferent fixed cancenttations of 4 Tinear miestype inhibitor (a> 1,80), Because B'=0, the velocity can be diven {0 {ero at [I]= 9, Thus the limiting plot sa vertical ine through the intersection point. 8) ea typeinntiion ‘where = 2.0 "ree =) bans cent = (;-) of coat sone = ( Ki w Fig. IV, Spey, and 1/oans intercept replots of reciprocal plot data fora linear imived ype inhibitor where a> 1 and BO. Fig. 2. Dixon plot: (2) 1/o versus (I a if The characteristics of the plot ae alos! i tive inhibitor (except the intersection poi {oes ot po through the ign as docs he 1 fixed concentrations of substrate hat obtained foe a pure compet 10 1/ Vy) (8) Sie opt which re competitive ihition).