Dosis ni ri pi pi corregido zi

0 20 0 0
1.4 20 1 0.05 0.050 -1.645
1.8 20 2 0.1 0.100 -1.282
2.4 20 9 0.45 0.450 -0.126
3 20 14 0.7 0.700 0.524
4 20 18 0.9 0.900 1.282
5.2 20 20 1 1.000 #VALUE!

m= 6.762
b= 2.234

CL50= 2.565

yi calculada zi φzi Pi acumulada 1-Pi acumulada ωi

3.222 -1.778 0.082 0.038 0.962 0.186
3.960 -1.040 0.232 0.149 0.851 0.425
4.805 -0.195 0.391 0.423 0.577 0.628
5.461 0.461 0.359 0.677 0.323 0.589
 6.305 1.305 0.170 0.904 0.096 0.335

b=
m=

Limites de confianza
Sμ^2= 0.0005
Sμ= 0.0220
t= 1.9773 N= 140
μsup= 0.4516
μinf= 0.3645

LSC= 2.829
LIC= 2.314
yi xi 1/raíz 2 *pi Exp
0.39894228
3.355 0.146 0.206
3.718 0.255 0.582
4.874 0.380 0.981
5.524 0.477 0.899
6.282 0.602 0.427
#VALUE! 0.716

vi niωi niωixi niωixi^2 niωivi niωivixi niωi(xi-xpromedio)^2

3.372 3.721 0.544 0.079 12.546 1.833 0.233
3.748 8.491 2.168 0.553 31.828 8.125 0.169
4.875 12.553 4.773 1.815 61.191 23.265 0.003
5.523 11.773 5.617 2.680 65.029 31.027 0.077
 6.281 6.696 4.031 2.427 42.057 25.321 0.284

Ʃ= 43.234 17.132 7.555 212.650 89.571 0.765

α1= 2.173 CL50(1)= 2.559

β1= 6.929 μ= 0.408
Promedio x= 0.396

CL50(1)= 2.559±0.0652 mg/kg

IC95(1)= 2.314-2.829 mg/kg
 Concentración ni ri pi Abbott Miller y Ta zi yi
0 10 0 0
5 10 0 0 0.000 #VALUE! #VALUE!
10 9 0 0 0.000 0.056 -1.593 3.407
20 10 2 0.2 0.200 0.200 -0.842 4.158
40 10 5 0.5 0.500 0.500 0.000 5.000
80 10 7 0.7 0.700 0.700 0.524 5.524
160 10 9 0.9 0.900 0.900 1.282 6.282
320 11 10 0.9 0.909 0.909 1.335 6.335

Concentración ni ri pi Abbott Miller y Ta zi yi

0 10 2 0.2
5 10 1 0.1 -0.125 #VALUE! #VALUE!
10 10 3 0.3 0.125 0.125 -1.150 3.850
20 10 4 0.4 0.250 0.250 -0.674 4.326
40 10 6 0.6 0.500 0.500 0.000 5.000
80 10 8 0.8 0.750 0.750 0.674 5.674
160 10 9 0.9 0.875 0.875 1.150 6.150
320 10 10 1 1.000 0.950 1.645 6.645

xi yi
1.000 3.407
1.000 3.85
1.301 4.158
1.301 4.326
1.602 5
1.602 5
1.903 5.624
1.903 5.674
2.204 6.282
2.204 6.15
2.505 6.335
2.505 6.645

β m 1.910 CL50 40.649

α b 1.927 CL16 12.324
CL84 134.075
S 60.875
N'= 50 EEM 12.175
t= 2.011 LIC 16.170
LSC 65.129
xi

0.699
1.000
1.301
1.602
1.903
2.204
2.505

xi

0.699
1.000
1.301
1.602
1.903
2.204
2.505

CL50= 7.961±2.134
mg/mL
IC95= 3.750-12.1mg/mL
2 Concentración ni ri pi pi corregidLog C pi calculad
0 20 0 0
2 20 2 0.1 0.100 0.301 0.171
10 20 10 0.5 0.500 1.000 0.497
20 20 16 0.8 0.800 1.301 0.637
100 20 18 0.9 0.900 2.000 0.963
140 20 20 1 1.000 2.146 1.031

m= 0.466 N'=60
b= 0.031
pi calculada corregida

0.171 m= 0.466
0.497 b= 0.031
0.637
0.963
1.031 DE50= 10.146
DE16= 1.891
DE84= 54.436
S= 5.365
E= 0.358
FCL50= 1.823
LSC= 18.502
LIC= 5.564
Concentración ni ri pi Abbott Miller y Ta zi yi
0.7 10 1 0.1 0.100 0.1 -1.282 3.718
0.9 10 1 0.1 0.000 0.100 -1.282 3.718
1.2 10 3 0.3 0.222 0.300 -0.524 4.476
1.5 10 5 0.5 0.444 0.500 0.000 5.000
2 9 9 1 1.000 0.944 1.593 6.593
2.6 10 10 1 1.000 #VALUE! #VALUE!

Concentración ni ri pi Abbott Miller y Ta zi yi

0.7 10 0 0 0.000 0.050 -1.645 3.355
0.9 10 1 0.1 0.100 0.100 -1.282 3.718
1.2 10 3 0.3 0.300 0.300 -0.524 4.476
1.5 10 5 0.5 0.500 0.500 0.000 5.000
2 10 9 0.9 0.900 0.900 1.282 6.282
2.6 10 10 1 1.000 0.950 1.645 6.645

xi yi
-0.155 3.718
-0.155 3.355
-0.046 3.718 CL50= 1.356
-0.046 3.718 CL16= 0.934
0.079 4.476 CL84= 1.965 N'= 60
0.079 4.476 t= 1.980
0.176 5 S= 3.275
0.176 5 EEM= 0.598
0.301 6.593
0.301 6.282 LIC= 0.171
0.415 6.645 LSC= 2.540
m= 6.145
b= 4.188
xi
-0.155
-0.046
0.079
0.176
0.301

xi
-0.155
-0.046
0.079
0.176
0.301
0.415
Dosis No. De levantamientos Gpo1 No. De levantamientos Gpo2 %Efecto Gpo 1
Control 47 49 97.917
1 45 44 93.750
10 32 36 66.667
30 27 27 56.250
100 16 15 33.333
300 8 7 16.667
1000 1 0 2.083

Valor de control= 48

x y
0.000 1.176
0.000 1.041
1.000 0.301
1.000 0.477 1.500
1.477 0.109 R² = 0.957
1.000
1.477 0.109
2.000 -0.301 0.500
2.000 -0.342
2.477 -0.699 0.000
0.000 0.5
2.477 -0.768
-0.500
3.000 -1.672
3 Err:502 -1.000

-1.500

-2.000
%Efecto Gpo 2 E/Emax-E Gpo 1 E/Emax-E Gpo 2 log(E/Emax-E Gpo 1) log(E/Emax-E Gpo 2)
102.083 47.000 -49.000 Err:502
91.667 15.000 11.000 1.176 1.041
75.000 2.000 3.000 0.301 0.477
56.250 1.286 1.286 0.109 0.109
31.250 0.500 0.455 -0.301 -0.342
14.583 0.200 0.171 -0.699 -0.768
0.000 0.021 0.000 -1.672 Err:502

m= -0.825
b= 1.217
r^2= 0.958

Chart Title
1.500

1.000 R² = 0.957821380133747

0.500

0.000
0.000 0.500 1.000 1.500 2.000 2.500 3.000 3.500
-0.500

-1.000

-1.500

-2.000
Log C Gpo1 Log C Gpo2

0.000 0.000
1.000 1.000
1.477 1.477
2.000 2.000
2.477 2.477
3.000 3.000

DI50= 29.823
Dosis(mg/kg) %Efecto Log D E/Emax-E Log(E/Emax-E)
5.00E-07 30 -6.301 0.429 -0.368 Lineal
5.00E-07 30 -6.301 0.429 -0.368 90
5.00E-07 32 -6.301 0.471 -0.327 80
1.00E-06 46 -6.000 0.852 -0.070 f(x) = 9190104.16666667 x + 34.66276041
70 R² = 0.845813624143716
1.00E-06 45 -6.000 0.818 -0.087
60
1.00E-06 46 -6.000 0.852 -0.070
2.00E-06 59 -5.699 1.439 0.158 50
2.00E-06 61 -5.699 1.564 0.194 40
2.00E-06 59 -5.699 1.439 0.158 30
3.00E-06 65 -5.523 1.857 0.269
20
3.00E-06 69 -5.523 2.226 0.347
10
3.00E-06 70 -5.523 2.333 0.368
5.00E-06 78 -5.301 3.545 0.550 0
0.00E+00 1.00E-06 2.00E-06 3.00E-06 4.00E-06
5.00E-06 74 -5.301 2.846 0.454
5.00E-06 73 -5.301 2.704 0.432

Semilog

DI50= 1.273E-06
f(x) = 45.0560639750301 x + 315.5383752484
R² = 0.986017599266336

-6.400 -6.200 -6.000 -5.800 -5.600

Hill

f(x) = 0.838188736376289 x + 4.9413661879

R² = 0.986028866089885

-6.400 -6.200 -6.000 -5.800 -5.600

 Lineal

90104.16666667 x + 34.6627604166667
5813624143716

2.00E-06 3.00E-06 4.00E-06 5.00E-06 6.00E-06

Semilog
90
80
639750301 x + 315.538375248483 70
599266336
60
50 m= 45.056
40 b= 315.538
r^2= 0.986018
30
20
10
0
6.000 -5.800 -5.600 -5.400 -5.200

Hill
0.800

0.600

188736376289 x + 4.94136618799498
m= 0.838
0.400
28866089885 b= 4.941
0.200 r^2= 0.986029

0.000
-6.000 -5.800 -5.600 -5.400 -5.200
-0.200

-0.400

-0.600
-0.400

-0.600
 Dosis(mg/kg)
Tiempo (min) Control 10 31.6 56.2 100
0 120 90 56 59 58
10 120 89 38 83 19
20 120 112 29 96 10
30 120 88 53 70 4
40 120 94 78 62 4
50 120 101 62 17 2
60 120 116 59 48 2
70 120 97 57 42 0
80 120 103 27 23 0
90 120 80 73 33 0
100 120 91 80 25 0
110 120 107 72 17 0
120 120 86 74 21 0

Tiempo (min) Control (C1+C2)/2 t2-t1 (C1+C2)/2*t2-t1 Dosis(10 mg/kg)

0 120 90
10 120 120 10 1200 89
20 120 120 10 1200 112
30 120 120 10 1200 88
40 120 120 10 1200 94
50 120 120 10 1200 101
60 120 120 10 1200 116
70 120 120 10 1200 97
80 120 120 10 1200 103
90 120 120 10 1200 80
100 120 120 10 1200 91
110 120 120 10 1200 107
120 120 120 10 1200 86
ABC= 14400

Dosis(mg/kg) log dosis %Efecto E/Emax-E Log(E/Emax-E)

10 1.000 77.917 3.528 0.548
31.6 1.500 47.708 0.912 -0.040
56.2 1.750 42.083 0.727 -0.139
100 2.000 24.861 0.331 -0.480

DE50= 45.963
 Control 10 31.6 56.2 100
ABC 14400 11220 6870 6060 3580
%E 100 77.917 47.708 42.083 24.861
100-%E 0.000 22.083 52.292 57.917 75.139

(C1+C2)/2 t2-t1 (C1+C2)/2*t2-t1 Dosis(31.6 mg/kg) (C1+C2)/2 t2-t1 (C1+C2)/2*t2-t1

56
89.5 10 895 38 47 10 470
101 10 1010 29 42.5 10 425
89 10 890 53 54.5 10 545
92 10 920 78 67 10 670
95.5 10 955 62 59 10 590
103 10 1030 59 57.5 10 575
93.5 10 935 57 56.5 10 565
96.5 10 965 27 41.5 10 415
85 10 850 73 64.5 10 645
90.5 10 905 80 68 10 680
98.5 10 985 72 64 10 640
88 10 880 74 65 10 650
ABC= 11220 ABC= 6870

Lineal Semilog Hill

m -0.533 -51.280 -0.991
b 74.488 128.260 1.520
r^2 0.981 0.891 0.979
Dosis(56.2 mg/kg) (C1+C2)/2 t2-t1 (C1+C2)/2*t2-t1 Dosis(100 mg/kg)
59 58
83 71 10 710 19
96 76 10 760 10
70 63 10 630 4
62 59 10 590 4
17 36.5 10 365 2
48 52 10 520 2
42 49 10 490 0
23 39.5 10 395 0
33 44.5 10 445 0
25 40.5 10 405 0
17 36.5 10 365 0
21 38.5 10 385 0
ABC= 6060
(C1+C2)/2 t2-t1 (C1+C2)/2*t2-t1

39 10 390
33 10 330
30 10 300
30 10 300
29 10 290
29 10 290
28 10 280
28 10 280
28 10 280
28 10 280
28 10 280
28 10 280
ABC= 3580
 Contracción (g fuerza) Contracción (g fuerza) Datos Corregidos
Concentración (M) Experimento 1 Experimento 2 Experimento 1 Experimento 2
Basal 0.9769 0.8922
0.1 0.9862 0.9126 0.0093 0.0204
0.3 1.0746 1.0373 0.0977 0.1451
1 1.6524 1.3236 0.6755 0.4314
3 1.8803 1.3727 0.9034 0.4805
10 1.8871 1.3756 0.9102 0.4834
ACh 1.9034 1.386 0.9265 0.4938

Concentración (M) log dosis %Efecto E/Emax-E Log(E/Emax-E)

0.1 -1.000 1.004 0.010 -1.994
0.1 -1.000 4.131 0.043 -1.366
0.3 -0.523 10.545 0.118 -0.929
0.3 -0.523 29.384 0.416 -0.381
1 0.000 72.909 2.691 0.430
1 0.000 87.363 6.913 0.840
3 0.477 97.507 39.108 1.592
3 0.477 97.307 36.128 1.558
10 1.000 98.241 55.840 1.747
10 1.000 97.894 46.481 1.667
 %Efecto
Experimento 1 Experimento 2

1.004 4.131
10.545 29.384
72.909 87.363
97.507 97.307
98.241 97.894
100.000 100.000

Lineal Semilog Hill

m 7.295 53.692 1.798
b 38.620 60.120 0.333
r^2 0.440 0.869 0.922

CE50= 0.653
 No. de levantamientos
Dosis (mg/kg)
Control 10 30 56.5 100
67 58 52 38 35
86 57 45 40 27
68 58 53 45 26
80 65 45 37 36
59 56 49 39 31
82 41 47 33
47

Valor de control= 69.857

Dosis(mg/kg) %Efecto E/Emax-E Log(E/Emax-E) LogC

10 83.027 4.892 0.689 1.000
10 81.595 4.433 0.647 1.000
10 83.027 4.892 0.689 1.000
10 93.047 13.382 1.127 1.000 m
10 80.164 4.041 0.607 1.000 b
10 58.691 1.421 0.153 1.000 r^2
30 74.438 2.912 0.464 1.477
30 64.417 1.810 0.258 1.477
30 75.869 3.144 0.497 1.477
30 64.417 1.810 0.258 1.477 DI50=
30 70.143 2.349 0.371 1.477
30 67.280 2.056 0.313 1.477
56.5 54.397 1.193 0.077 1.752
56.5 57.260 1.340 0.127 1.752
56.5 64.417 1.810 0.258 1.752
56.5 52.965 1.126 0.052 1.752
56.5 55.828 1.264 0.102 1.752
100 50.102 1.004 0.002 2.000
100 38.650 0.630 -0.201 2.000
100 37.219 0.593 -0.227 2.000
100 51.534 1.063 0.027 2.000
100 44.376 0.798 -0.098 2.000
100 47.239 0.895 -0.048 2.000
 %Efecto
10 30 56.5 100
83.027 74.438 54.397 50.102
81.595 64.417 57.260 38.650
83.027 75.869 64.417 37.219
93.047 64.417 52.965 51.534
80.164 70.143 55.828 44.376
58.691 67.280 47.239

Lineal Semilog Hill

-0.385 -34.828 -0.742
81.852 116.990 1.416
0.784 0.770 0.741

82.669
 Desplazamientos g
Concentración (µg/mL) ACh 1/Concentración (µg/mL) Ach (1/A) Log C Ach Ach + A
0.001 1000 -3 0.2 0.1
0.01 100 -2 0.4 0.2
0.1 10 -1 1.1 0.7
1 1 0 1.8 1.5
10 0.1 1 2.1 1.7
100 0.01 2 2.1 1.7

%Efecto E/Emax-E
Ach Ach + A Ach + B Ach
9.524 4.762 4.762 0.105
19.048 9.524 14.286 0.235
52.381 33.333 28.571 1.100
85.714 71.429 57.143 6.000
100.000 80.952 95.238 #DIV/0!
100.000 80.952 100.000 #DIV/0!

ACh Ach+A
1.000 1.000
f(x) = 0.433268635397856 x + 0.0624566963915698
f(x) = 0.593740618222601 x + 0.693968777384579
0.500 R² = 0.926767464484493
0.500
R² = 0.977410887611708
0.000 0.000
-3.5 -3 -2.5 -2 -1.5 -1 -0.5 0 -4 -3 -2 -1 0 1 2 3
-0.500 -0.500

-1.000 -1.000

-1.500
-1.500

1/(A) vs 1/%E
0.250

0.200 f(x) = 0.000186188384882968

0.000188972729449122 x + 0.0294716843376044
0.0238384184302978 ACh
R² = 0.871686277068849
0.958303229306025
Linear (ACh)
0.150
Ach+A
1/%E

0.100 Linear (Ach+A)

f(x) = 8.93745023608548E-05 x + 0.0181587787399011
R² = 0.885792702342807 ACh+B
0.050 Linear (ACh+B)

0.000
0 200 400 600 800 1000 1200
1/(A)
plazamientos g
Ach + B
0.1
0.3
0.6
1.2
2
2.1

E/Emax-E Log(E/Emax-E) 1/%E

Ach + A Ach + B Ach Ach + A Ach + B Ach
0.050 0.050 -0.978 -1.301 -1.301 0.105
0.105 0.167 -0.628 -0.978 -0.778 0.053
0.500 0.400 0.041 -0.301 -0.398 0.019
2.500 1.333 0.778 0.398 0.125 0.012
4.25 20 #DIV/0! 0.628 1.301 0.010
4.25 #DIV/0! #DIV/0! 0.628 #DIV/0! 0.010

Ach+B Ach
m= 0.594
1.500
+ 0.0624566963915698 b= 0.694
1.000 r^2= 0.977
f(x) = 0.610720996964787 x + 0.400490492475311
R² = 0.945723791014911 0.500 CE50= 0.068
1 2 3
0.000
-3.5 -3 -2.5 -2 -1.5 -1 -0.5 0 0.5 1 1.5
-0.500

-1.000

-1.500

Ach Ach + A Ach + B

m= 9.00E-05 2.00E-04 2.00E-04
ACh b= 0.018 0.024 0.030
Linear (ACh)
r^2= 0.886 0.958 0.872
Ach+A
Linear (Ach+A) Emax= 54.945 41.667 33.898
ACh+B x= -2.02E+02 -1.20E+02 -1.48E+02
Linear (ACh+B)
Antagonistas no competitivos
 1/%E
Ach + A Ach + B
0.210 0.210
0.105 0.070
0.030 0.035
0.014 0.018
0.012 0.011
0.012 0.010

Ach + A Ach + B
0.433 0.611
0.062 0.400
0.927 0.946
0.717 0.221 (µg/mL)
 %Efecto 1/%Efecto
Concentracion del Ligando (mg/mL) 1/[A] L L+B1 L+B2 L
1 1.000
1.7 0.588
3.2 0.313 13 12 0.077
5.6 0.179 23 17 0.043
10 0.100 42 28 0.024
17 0.059 68 14 37 0.015
32 0.031 85 20 51 0.012
56 0.018 100 55 66 0.010
100 0.010 100 66 84 0.010
170 0.006 90 95
320 0.003 99 100
560 0.002 100

f(x) = NaN x + NaN

R² = 0
(1/[A])/(1/%Efecto)
0.2
f(x) = 0.0382729825024883 x + 0.10413574389047
0.18 R² = 0.904623249940908
0.16
0.14
0.12
0.1
0.08
0.06
0.04
0.02
0
0 1 2 3 4 5 6 7 8 9

L Linear (L)
L+B1 Linear (L+B1)
Column H Linear (Column H)
 1/%Efecto
L+B1 L+B2

0.083
0.059
0.036
0.071 0.027
0.050 0.020
0.018 0.015
0.015 0.012
0.011 0.011
0.010 0.010
0.010

L L+B1 L+B2
m= 0.226 1.181 0.242
b= 0.004 0.004 0.011
Emax= 228.187 229.751 91.548
x= -0.019 -0.004 -0.045

B1 competitivo
 % Efecto de ACh + Am1
Conc(M) de Am1
Concentración (M) de ACh %Efecto Ach
Log C 1.00E-04 1.00E-03 1.00E-02
0.0001 -4.000 19
0.0003 -3.523 37
0.0005 -3.301 53
0.001 -3.000 69 18
0.003 -2.523 83 40
0.005 -2.301 58 17
0.01 -2.000 72 32
0.03 -1.523 85 49
0.05 -1.301 69 15
0.1 -1.000 80 39
0.3 -0.523 51
0.5 -0.301 63
1 0.000 83

r^2 b m [Am1]
Ach 0.981 200.124 45.246 0
Ach+Am1 (1.00E-04) 0.971 161.180 46.965 1.00E-04
Ach+Am1 (1.00E-03) 0.983 128.705 48.804 1.00E-03
Ach+Am1 (1.00E-02) 0.967 80.058 47.773 1.00E-02

pA2
3.000

2.500 f(x) = − 0.894419510421003 x + 4.44447048583184

R² = 0.996106385775443
2.000

1.500

1.000

0.500

0.000
1.5 2 2.5 3 3.5 4 4.5
 Semilog
f(x) = 46.9648290222209 x + 161.179986848403
f(x) = 45.2456097000418 x + 200.124073290249
R² = 0.971114845578409
R² = 0.980879014827882 f(x) = 47.7
f(x) = 48.8037369138153 x + 128.70547450846
R² = 0.983347017995234 R² = 0.966

-4.500 -4.000 -3.500 -3.000 -2.500 -2.000 -1.500 -1.0

ACh Linear (ACh) ACh+Am1(1.00E-04) Lin

Ach+Am1(1.00E-03) Linear (Ach+Am1(1.00E-03)) Ach+Am1 (1.00E-02) Lin

CE50 [Am1] A'/A (A'/A)-1

0.0005 A 0
0.0043 A'1 1.00E-04 8.926 7.926
0.0244 A'2 1.00E-03 50.733 49.733
0.2349 A'3 1.00E-02 488.435 487.435

b= 4.444
m= -0.894

pA2= 4.969

4 4.5
 90
48403
80
f(x) = 47.7730074163502 x + 80.0575442879257
369138153 x + 128.70547450846
017995234 R² = 0.966992005529287 70
60
50
40
30
20
10
0
-1.500 -1.000 -0.500 0.000

ACh+Am1(1.00E-04) Linear (ACh+Am1(1.00E-04))

Ach+Am1 (1.00E-02) Linear (Ach+Am1 (1.00E-02))

log((A'/A)-1) Menos log [B]

0.899 4
1.697 3
2.688 2
 % Efecto de ACh + BM1
Ach Conc(M) de BM1
%Efecto ACh
(M) 1 2 4
0.05 3.3 2.6 1.7 1
0.1 6.4 5 3.4 1.8
3.2 40.5 32 21.5 11.7
5.6 68.5 54.1 36.3 19.9
10 79.2 62.6 42 23
32 87.2 68.9 46.2 25.3
56 95.6 75.5 50.7 27.7
100 97.4 77 51.6 28.3

[BM1] Emax [BM1] -log [BM1]

Ach 0 97.4 Ach 0.000
Ach+BM1 1 77 Ach+BM1 (1M) 1.000 0.000
Ach+BM1 2 51.6 Ach+BM1 (2M) 2.000 -0.301
Ach+BM1 4 28.3 Ach+BM1 (4M) 4.000 -0.602

pA2
0.600

0.400
f(x) = − 1.60208647558357 x − 0.562593807971585
R² = 0.997381568432164
0.200

0.000
-0.700 -0.600 -0.500 -0.400 -0.300 -0.200 -0.100 0.000
-0.200

-0.400

-0.600

-0.800
Emax/Emax' (Emax/Emax') -1 log ((Emax/Emax')-1)
1.000
1.265 0.265 -0.577
1.888 0.888 -0.052
3.442 2.442 0.388

b -0.5626
m -1.6021
pA2` -0.351
 % Efecto de pilocarpina + berberina
Pilocarpina (M) %Efecto Pilocarpina Conc(M) de berberina
1.00E-05 3.00E-05 6.00E-05
1.00E-10 0.03 0.02 0.01 0.01
1.00E-09 0.23 0.13 0.07 0.05
1.00E-08 1.61 0.93 0.52 0.33
1.00E-07 10.4 6.21 3.59 2.28
1.00E-06 45.1 31.91 20.87 14.16
1.00E-05 85.33 76.84 65.13 53.87
1.00E-04 97.63 95.92 92.97 89.21
1.00E-03 99.66 99.4 98.94 98.32

Lineal
120

100 f(x)
f(x) == 73735.7984402193
84050.5875175889 xx ++ 32.2576669857137
77763.5542247479
81480.0264399865 28.1195064656788
23.9458297742797
20.6050574059607 Pilocarpina
R²
R² == 0.322205880745774
0.371638366917842
0.431214205629953
0.487643707554706
Linear (Pilocarpina)
pilocarpina + berberina (1.00E-05)
80 Linear (pilocarpina + berberina (1.00E-
05))
pilocarpina + berberina (3.00E-05)
60 Linear (pilocarpina + berberina (3.00E-
05))
pilocarpina + berberina (6.00E-05)
40 Linear (pilocarpina + berberina (6.00E-
05))

20

0
0.00E+00 2.00E-04 4.00E-04 6.00E-04 8.00E-04 1.00E-03 1.20E-03

r^2 b m [Pilocarpina]
Hill Pilocarpina 1.000 5.033 0.854 0.00E+00
Hill pilocarpina + berberina (1.00E-05) 1.000 4.759 0.848 1.00E-05
Hill pilocarpina + berberina (3.00E-05) 1.000 4.537 0.853 3.00E-05
Hill pilocarpina + berberina (6.00E-05) 1.000 4.233 0.833 6.00E-05

pA2
0.800

0.700 f(x) = − 1.00281076871963 x + 4.97809084366056

R² = 0.999886310200326
0.600

0.500

0.400

0.300

0.200

0.100
 R² = 0.999886310200326
0.600

0.500

0.400

0.300

0.200

0.100

0.000
4.100 4.200 4.300 4.400 4.500 4.600 4.700 4.800 4.900 5.000 5.100
-0.100
 Log(E/(Emax-E) Log C
Pilocarpina 1.00E-05 3.00E-05 6.00E-05 -10
-3.523 -3.699 -4.000 -4.000 -9
-2.637 -2.885 -3.155 -3.301 -8
-1.786 -2.027 -2.282 -2.480 -7
-0.935 -1.179 -1.429 -1.632 -6
-0.085 -0.329 -0.579 -0.783 -5
0.765 0.521 0.271 0.067 -4
1.615 1.371 1.121 0.917 -3
2.467 2.219 1.970 1.767

Semilog
120

100
f(x) = 17.5032142857143 x + 156.269642857143 pilocarpina
R² = 0.891044620060171
f(x) = 17.0004761904762 x + 149.423095238095 Linear (pilocarpina)
R² = 0.871721297137373
f(x) = 16.2871428571429 x + 141.128928571429 pilocarpina + berberina (1.00E-05)
na (1.00E-05) R²
f(x)= =0.845605293847371
15.5532142857143 x + 133.374642857143
80 Linear (pilocarpina + berberina
berberina (1.00E- R² = 0.819499274609028 (1.00E-05))
pilocarpina + berberina (3.00E-05)
na (3.00E-05) Linear (pilocarpina + berberina
60
berberina (3.00E- (3.00E-05))
pilocpilocarpina + berberina (6.00E-
na (6.00E-05) 05)
berberina (6.00E- 40 Linear (pilocpilocarpina + berberina
(6.00E-05))

20

0
-11 -10 -9 -8 -7 -6 -5 -4 -3 -2

CE50 [M] [Pilocarpina] `-log [Pilocarpina] A'/A (A'/A) -1

1.27E-06 A 0.000
2.43E-06 A'1 0.000 5.000 1.917 0.917
4.83E-06 A'2 0.000 4.523 3.801 2.801
8.27E-06 A'3 0.000 4.222 6.514 5.514

66056
b 4.978
m -1.003
pA2 4.964
4.800 4.900 5.000 5.100
 Hill
3.000

f(x) = 0.853468300435154 x + 5.03251050405595

f(x)
R² ==0.999976204306319
0.847682903221816 x + 4.75884854940686 2.000
f(x)==0.999978080352635
R² 0.853327126661761 x + 4.53645750802844
f(x)==0.999989089049977
R² 0.83278229539775 x + 4.23265907708416 pilocarpina
R² = 0.999450403494647 1.000 Linear (pilocarpina)
(1.00E-05)
rberina pilocarpina + berberina (1.00E-05)
0.000 Linear (pilocarpina + berberina
(3.00E-05) -11 -10 -9 -8 -7 -6 -5 -4 -3 -2 (1.00E-05))
rberina pilocarpina + berberina (3.00E-05)
-1.000 Linear (pilocarpina + berberina
rina (6.00E- (3.00E-05))
pilocarpina + berberina (6.00E-05)
-2.000
+ berberina Linear (pilocarpina + berberina
(6.00E-05))
-3.000

-4.000

-5.000

log ((A'/A)-1)

-0.038
0.447
0.741
 % Efecto de Serotonina + aceite
%Efecto Concentración (µg/mL) de aceite
Serotonina (µg/mL) Serotonina 30 100
1.00E-10 0.05 0.05 0.03
1.00E-09 0.32 0.28 0.21
1.00E-08 1.88 1.66 1.23
1.00E-07 10.36 9.11 6.76
1.00E-06 41.05 36.11 26.78
1.00E-05 80.76 71.04 52.67
1.00E-04 96.2 84.62 62.74
1.00E-03 99.35 87.39 64.8

[Aceite] Emax
Serotonina 0 99.35
Serotonina + aceite (30 µg/mL) 30 87.39
Serotonina + aceite (100 µg/mL) 100 64.8
Serotonina + aceite (300 µg/mL) 300 27.56

pA2
0.600

0.400
f(x) = − 1.2771245303593 x − 2.7751273140903
R² = 0.995003906713626 0.200

0.000
-2.600 -2.400 -2.200 -2.000 -1.800 -1.600 -1.400
-0.200

-0.400

-0.600

-0.800

-1.000
% Efecto de Serotonina + aceite
Concentración (µg/mL) de aceite
300
0.01
0.09
0.52
2.87
11.39
22.4
26.69
27.56

[Aceite] `-log [Aceite] Emax/Emax'

Serotonina 0 1
Serotonina + aceite (30 µg/mL) 30 -1.477 1.137
Serotonina + aceite (100 µg/mL) 100 -2.000 1.533
Serotonina + aceite (300 µg/mL) 300 -2.477 3.605

0.600

0.400
b -2.775
0.200 m -1.277
pA2` -2.173
0.000
-1.600 -1.400
-0.200

-0.400

-0.600

-0.800

-1.000
(Emax/Emax') -1 log ((Emax/Emax')-1)

0.137 -0.864
0.533 -0.273
2.605 0.416