6.

00
Absorban
Tubo cia Concentracion 5.00 f(x) = 6.50618087182824 x − 7.94410929039127E-16
505nm proteina R² = 1
00 4.00
1 0.247 1.61

Proteina
3.00
2 0.47 3.06
3 0.632 4.11 2.00
4 0.67 4.36
5 0.79 5.14 1.00

6 0.28
0.00
7 0.433 0.2 0.3 0.4 0.5 0.6 0.7

8 0.350 Absorbancia
9 0.547

y=6,5062x-8e-15 Ley de lambert-beer=A=e*C*L

y=mx+b
m=6,5062
C=A/e*L
A=absorcion Concentracion muestra A tubo 6
e=coeficiente de extinion molar
c=concentracion 6 0.252 6.5062 0.04
L=Longitud de mi celda 7 0.122 6.5062 0.02
8 0.061 6.5062 0.01
9 0.488 6.5062 0.08
10 0.229 6.5062 0.04
11 0.111 6.5062 0.02

Concentracion proteina
 Absorbancia vs proteina
4 x − 7.94410929039127E-16 6.00

5.00 f(x) = 6.50618087182824 x − 7.94410929039127E-16

R² = 1
4.00

3.00

2.00

0.5 0.6 0.7 0.8 0.9 1.00

Absorbancia
0.00
0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9

Promedio A Promedio b
0.145

0.276
na

16

0.7 0.8 0.9