Validación del método filtración por membrana

para análisis microbiológico de coliformes

totales y Escherichia coli en aguas marinas
Membrane filtration method validation to total coliforms and Escherichia coli
microbiological analysis in seawaters
Nombres:
Nicolás Acosta Cubillos
Michel Camila Garzón Pulido
Alejandro Ruíz Sánchez
Valeria Ariza Hernández
 Introducción

Agar Chromocult
para coliformes
totales y Validación del método filtración por
Escherichia coli membrana

“Confirmación, a través del examen y el aporte de evidencias objetivas, de que se cumplen

los requisitos particulares para un uso específico previsto”
 Metodología

01 02 Filtración de la muestra

10 ml de agua de
mar y se completó
a 100 ml con agua
destilada estéril

Mx nivel superficial en frascos

Schoot de 250 ml estériles
 Metodología
Siembra Agar Chromocult

Colonias de coliformes de
color rojo asalmonado

Temperatura de
35+/-0.5°C por Colonias de E. coli
un periodo de adquieren un color entre
azul oscuro y violeta
24 horas.

Medio de cultivo cromógeno diferencial para el

análisis microbiológico de muestras de agua. En 24
horas permite la detección de E. coli y bacterias
coliformes del agua potable
 Pruebas confirmativas
Proceso de validación de coliformes totales
y E. Coli

Oxidasa

Agar VRB (Violeta

cristal-Rojo
neutro-Bilis-Lactosa)

Detección y recuento de E. coli Coliformes

colonias de bacterias
coliformes de alimentos y
piensos, agua y otros Los datos de las variables repetibilidad y
reproducibilidad fueron obtenidos a través de MINITAB
materiales.
 Resultados

Tabla 1.. Extraida de Membrane filtration method validation to total coliforms and Escherichia coli
microbiological analysis in seawaters
 Resultados

Tabla 2.. Extraida de Membrane filtration method validation to total coliforms and Escherichia coli
microbiological analysis in seawaters
 Resultados

Tabla 3.. Extraida de Membrane filtration method validation to total coliforms and Escherichia coli
microbiological analysis in seawaters
 Resultados

Tabla 4.. Extraida de Membrane filtration method validation to total coliforms and Escherichia coli
microbiological analysis in seawaters
 Resultados

Tabla 5.. Extraida de Membrane filtration method validation to total coliforms and Escherichia coli
microbiological analysis in seawaters
 Resultados

Tabla 6.. Extraida de Membrane filtration method validation to total coliforms and Escherichia coli
microbiological analysis in seawaters
 DISCUSIÓN Y CONCLUSIÓN

❖ Determinación de parámetros de repetibilidad

❖ Las variables mostraron diferencias significativas, para el indicador
bacteriológico de coliformes totales
❖ Se presenta un rechazo de hipótesis
❖ Evaluación de reproducibilidad

Durante el desarrollo del proceso de validación de coliformes totales y E. coli, el método

normalizado filtración por membrana en agar Chromocult fue aplicable para el análisis del
analito.
 Normatividad
DECRETO 2811 DE
1974 LEY 56 DE 1987

DECRETO LEY DECRETO 644 DE

2324 DE 1984 1990

DECRETO 1681 DE DECRETO 2858

1978 DE 1981
 Normatividad
DECRETO 2105 DE
1983
DOCUMENTO
CONPES 1750 DE
1995
LEY 2324 DEL 18
DE SEPTIEMBRE
DE 1984 LEY 99 DE 1993
GOBIERNO
NACIONAL
 Normatividad
Decreto 321 de 1999

Decreto 3100 de LEY 12 DE 1992

2003

Decreto 3440 de DECRETO 2190 DE

2004 1995
 REFERENCIAS
● López, K. (2015). Validación del método filtración por membrana para análisis microbiológico de
coliformes totales y Escherichia coli en aguas marinas. Bol. Cient. CIOH 2015, 33: 215–220.
Disponible en:
https://www.cioh.org.co/dev/publicaciones/acceso_dev.php?nbol=cioh_bcc3313.pdf
● http://www.upme.gov.co/guia_ambiental/carbon/gestion/politica/normativ/normativ.htm#BM2
_12_Normatividad_sobre_mares_y_costas
● https://repository.ean.edu.co/bitstream/handle/10882/1615/NormasAmbientales.pdf;js
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