Resultados

(Los presentes resultados se calcularon en el programa EXCEL gracias a las indicaciones de los
profesores de laboratorio).

Resultados de tensión de músculo liso vascular normalizada para distintas concentraciones de histamina

[H] (nM) [H] (M) log [H] (M) E0 (%) Corrección E0 log E0
1 0.000000001 -9.00000 0.0200 0.00020 -3.69877
4 0.000000004 -8.39794 0.0799 0.00080 -3.09700
16 0.000000016 -7.79588 0.3190 0.00320 -2.49471
64 0.000000064 -7.19382 1.2638 0.01280 -1.89269
256 0.000000256 -6.59176 4.8706 0.05121 -1.29062
1024 0.000001024 -5.98970 16.9987 0.20486 -0.68854
4000 0.000004 -5.39794 44.4444 0.80036 -0.09672
16000 0.000016 -4.79588 76.1905 3.20337 0.50561
64000 0.000064 -4.19382 92.7536 12.84427 1.10871
256000 0.000256 -3.59176 98.0843 51.87724 1.71498
1024000 0.001024 -2.98970 99.5141 215.91256 2.33428
4096000 0.004096 -2.38764 99.8781 1030.73375 3.01315
20000000 0.02 -1.69897 99.9750

Resultados de tensión de músculo liso vascular normalizada para distintas concentraciones de 2-PEA

[H] (nM) [H] (M) log [H] (M) E0 (%) Corrección E0 log E0
1 0.000000001 -9.00000 0.00050 5.05003E-06 -5.29671
4 0.000000004 -8.39794 0.00200 2.02004E-05 -4.69464
16 0.000000016 -7.79588 0.00800 8.08065E-05 -4.09255
64 0.000000064 -7.19382 0.03200 3.23304E-04 -3.49039
256 0.000000256 -6.59176 0.12780 1.29245E-03 -2.88859
1024 0.000001024 -5.98970 0.50940 5.17155E-03 -2.28638
4000 0.000004 -5.39794 1.96080 2.02042E-02 -1.69456
16000 0.000016 -4.79588 7.40740 8.08646E-02 -1.09224
64000 0.000064 -4.19382 24.24240 3.24237E-01 -0.48914
256000 0.000256 -3.59176 56.14040 1.30957E+00 0.11713
1024000 0.001024 -2.98970 83.66010 5.45024E+00 0.73642
4096000 0.004096 -2.38764 95.34450 2.60120E+01 1.41517
20000000 0.02 -1.69897 99.00990
*E = x10exponente
 Resultados de tensión de músculo liso vascular normalizada para distintas concentraciones de 2-TEA

[H] (nM) [H] (M) log [H] (M) E0 (%) Corrección E0 log E0
1 0.000000001 -9.00000 0.01310 0.00022 -3.66069
4 0.000000004 -8.39794 0.05250 0.00088 -3.05751
16 0.000000016 -7.79588 0.20930 0.00350 -2.45576
64 0.000000064 -7.19382 0.82870 0.01401 -1.85361
256 0.000000256 -6.59176 3.18280 0.05603 -1.25157
1024 0.000001024 -5.98970 10.98320 0.22413 -0.64949
4000 0.000004 -5.39794 28.00470 0.87565 -0.05767
16000 0.000016 -4.79588 46.66990 3.50469 0.54465
64000 0.000064 -4.19382 56.00120 14.05265 1.14776
256000 0.000256 -3.59176 58.94770 56.75688 1.75402
1024000 0.001024 -2.98970 59.73340 236.19375 2.37327
4096000 0.004096 -2.38764 59.93310 1126.56203 3.05176
20000000 0.02 -1.69897 59.98630

Log E0 vs Log []
4.00000

3.00000

2.00000

1.00000

0.00000
-10.0000 -9.0000 -8.0000 -7.0000 -6.0000 -5.0000 -4.0000 -3.0000 -2.0000
Log E0 (%)

Histamina
-1.00000 2-PEA
2-TEA

-2.00000

-3.00000

-4.00000

-5.00000

-6.00000

Log [] (M)
 Histamina 2-PEA 2-TEA
A 5.3565 3.7604 5.3955
B 1.0078 1.0080 1.0078
r 0.9999 0.9999 0.9999
R2 0.9998 0.9998 0.9998

Histamina 2-PEA 2-TEA

5.3565 3.7604 5.3955
CE50 ( ) ( ) ( )
=10 −1.0078
¿ 10 −1.0080
¿ 10 −1.0078
= 4.8412x10-6 1.8597x10-4 4.4285x10-6
Emax 99.9750 99.0099 59.9863
DE50 y = mx+B y = mx+B y = mx+B
(DE50 = y  x= 0) y = 1.0078x + 5.3565 y = 1.0080x + 3.7604 y = 1.0078x + 5.3955
y = 1.0078(0) + 5.3565 y = 1.0080(0) + 3.7604 y = 1.0078(0) + 5.3955
y= 5.3565 y= 3.7604 y= 5.3955
DE50= 227247.9635 M DE50= 5759.7018 M DE50=248599.3564 M

2-PEA 2-TEA
ER 99.0099 59.9863
= =
99.9750 99.9750

=0.9903 =0.5900
−4
PR 1.8597 x 10 4.4285 x 10−6
= =
4.8412 x 10−6 4.8412 x 10−6

=0.3841x10-2 =0.9147
 100.0000

90.0000

80.0000

70.0000

60.0000
E0 (%)

50.0000
Histamina
40.0000 2-PEA
2-TEA
30.0000

20.0000

10.0000

0.0000
-10.0000 -9.0000 -8.0000 -7.0000 -6.0000 -5.0000 -4.0000 -3.0000 -2.0000 -1.0000
Log [] (M)