Solutions: Chapter 11 127 t= 157 min Answer: 15.7 min 11.7 Growth parameters for recombinant E, coli @) ‘The average rate-equal area construction is used to determine growth rates from the concentration data. The data and ‘calculations ate tabulated below. ‘Time, #() tke) Ax (ke) arch) Sas kg me bh) 8 020 oot 033 003 ‘ns O21 oor ou, 0.06 ba sz 010 028 040 ons 032 oas 025 0.60, 10 oar 053 050 1.06 1s 108. 110 050 220 me 210 2a 050 4.064 a3 aa 248 030 827 a8 ss 250 020 1250 30 5A 1.50 0.10 15,00 3d 109 070 0.10 7.00 32 16 010 030 033 3s 17 0.10. 020 0.50 37 ué ‘The values of 4*/+ are plotted as a function of time below according to the method described on p 263. 16 14 2 10 kg v3 tet Ax at Time (h)128 Solutions: Chapter 11 Results for 4¥/g, read from the average rate-equal area curve and the calculated specific growth rates are listed below. Time, ¢(h) x= Bigg kg tr} wal Pigg hy 00 0 0 033 os 024 05 020 091 O75 0.45 141 10 070 149 1s 15 130 20 33 1s7 25 65 147 28 toa 131 30 145 134 34 110 Lot 32 35 030 35 03 0.03 a7 08 -001 ‘Values ofthe specific prow rate ae plotted asa function of time below. 20 s 1 é x é 2 10 1. 05 ° 1 2 2 4 Ti ® As expected for miost batch cell cultures, growth occurs at around the miaximiumn specific growth rate for most of the Culture period. Taking the average of the values of y between times 0.75 h and 3, dgnax = 1.50: 0.05 br}, where 0.05 is the standard deviation. Answer: 1501 © From Bq, (11.49) the observed biomass yield Yig at any point in time is equal to the ratio of the observed growth and substrate consumption rates. The average tate-equal area construction is used to determine the Substrate consumption cates 4°54 4 Bom the concentration date, x shown below.Solutions: Chapter 11 129 ‘Time, (0) sgn) ‘As (ke m3) At(h) AS (eg 3b) 00 3a 02 033 ost As ae 00 a7 00 ay ous 02 02s 080 ous m8 -03 0.25 120 is 3 “10 050 2.00 ls Bs 26 050 $20 20 207 59 950 109 - 25 157 “55 030 183 28 108. 50 0.20 250 1% $2. 335 010 355 34 a tas dio 45 32. ag 02 030 067 as bd 00 020 00 ~ 37 00 ‘The values of ~AS/, are plotted asa function of time below according to the method described on p 263. Time (ri) Results for ~%jy read from the average rate-equal area curve are listed below, together with the instantaneous - biomass yield coefficients calculated using Bq. (11.49) and the values of rx from (a).‘Time, #(b) r= S8(a¢ (eg mr tr!) Yigg = "rg Oks ke") 00 o4 o 033 os 0.10 os 06 033 075 10 04s 10 16 044 15 30 0.50 20 14 04s 25 13.9 047 28 22 047 30 33.0 046 3 nS 040 32 70 0.50 35 o4 075 37 0 - During the exponential growth phase between 0.75 h and 3 h when 1: = jimax, Yxs is approximately constant with an average value of 0.46: 0.02 kg kg"!, where 0.02 is the standard deviation, Answer: 046 kg ke"); ¥g is approximately constant during exponential growth 11.8 Growth parameters for hairy roots @ ‘The mid-point slope method is used to determine rates from the concentration data. ‘The growth data are listed and plotted below according to the method described on pp 264-265. Values of ((r)se~ (e)i-a are read from the graph, ‘Time, ¢ @) ‘Biomass concentration, x (g I!) € [@ue-@ed et) ° 068 3 = 5 195 3 30 10 421 5 36 15 554 5 38