Datos TP2 para Alumnos

Method: TAI 2015
Software: 2.7.0
Sample ID User ID Date Time Measure TypGain Conc. Units
Blank Default 4/25/2016 06:32 p.m. Blank 7.56 NaN mM
10 Default 4/25/2016 06:33 p.m. Measure 7.24 NaN mM
9 Default 4/25/2016 06:34 p.m. Measure 10 NaN mM
Blank Default 4/25/2016 07:43 p.m. Blank 3.08 NaN mM
Min Gain Max gain ratDiluton factoNorm. coeff. Formula RFU nm1 RFU1
10 3 1.00E+00 1 nm1 0 511 0
10 3 1.00E+00 1 nm1 86.8 511 86.8
10 3 1.00E+00 1 nm1 0 511 0
10 3 1.00E+00 1 nm1 217.5 511 217.5
10 3 1.00E+00 1 nm1 0 511 0
10 3 1.00E+00 1 nm1 0 511 0
10 3 1.00E+00 1 nm1 0 511 0
10 3 1.00E+00 1 nm1 0 511 0
10 3 1.00E+00 1 nm1 0 511 0
10 3 1.00E+00 1 nm1 0 511 0
10 3 1.00E+00 1 nm1 981.4 511 981.4
10 3 1.00E+00 1 nm1 11087.7 511 11087.7
10 3 1.00E+00 1 nm1 9559.8 511 9559.8
10 3 1.00E+00 1 nm1 21032.4 511 21032.4
10 3 1.00E+00 1 nm1 20031.9 511 20031.9
10 3 1.00E+00 1 nm1 0 511 0
10 3 1.00E+00 1 nm1 0 511 0
10 3 1.00E+00 1 nm1 0 511 0
10 3 1.00E+00 1 nm1 0 511 0
10 3 1.00E+00 1 nm1 0 511 0
10 3 1.00E+00 1 nm1 0 511 0
10 3 1.00E+00 1 nm1 5288.6 511 5288.6
10 3 1.00E+00 1 nm1 18531.6 511 18531.6
10 3 1.00E+00 1 nm1 32838.2 511 32838.2
10 3 1.00E+00 1 nm1 30267.7 511 30267.7
10 3 1.00E+00 1 nm1 23631.7 511 23631.7
10 3 1.00E+00 1 nm1 7430.8 511 7430.8
Delta1 nm2 RFU2 Delta2 nm3 RFU3 Delta3 nm4
NaN NaN NaN NaN NaN NaN NaN NaN
RFU4 Delta4 Std curve Std 0 conc Std 0 RFU Std 1 conc Std 1 RFU Std 2 conc
NaN NaN 1 NaN NaN NaN NaN NaN
Std 2 RFU Std 3 conc Std 3 RFU Std 4 conc Std 4 RFU Std 5 conc Std 5 RFU Std 6 conc
Std 6 RFU Std 7 conc Std 7 RFU Serial numbeFirmware Software LED 395
NaN NaN NaN USB2G38302USB2000 2.502.7.0 Blue 0
NaN NaN NaN USB2G38302USB2000 2.502.7.0 Blue NaN
NaN NaN NaN USB2G38302USB2000 2.502.7.0 Blue 0
399 403 407 411 415 419 423 427
0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0
431 435 439 443 447 451 455 459
0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0
463 467 471 475 479 483 487 491
0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0
495 499 503 507 511 515 519 523
0 0 0 0 0 0 0 0
NaN 0 0 21.3 84.7 77.7 55.6 33.4
NaN 0 0 0 0 0 0 0
NaN 423.7 399.1 319 222.7 193.7 171.3 148
NaN 0 0 0 0 0 0 0
NaN 0 0 0 0 0 0 0
NaN 0 0 0 0 0 0 0
NaN 0 0 0 0 0 0 0
NaN 0 0 0 0 0 0 0
NaN 0 0 0 0 0 0 0
NaN 0 0 384.6 958.2 973.9 866.3 763.6
NaN 5141.5 7670.5 9805.1 11064.7 10614.9 9567.6 8556.2
NaN 4122.9 6372.4 8329.3 9534.7 9166.6 8274 7406
NaN 9620.2 14479.5 18557.2 20984.9 20170.7 18262.5 16417.1
NaN 0 5010.5 12996.5 19768.2 19799.2 18401.9 17171.7
0 0 0 0 0 0 0 0
NaN 0 0 0 0 0 0 0
NaN 0 0 0 0 0 0 0
NaN 0 0 0 0 0 0 0
NaN 0 0 0 0 0 0 0
NaN 0 0 0 0 0 0 0
NaN 4161.3 4870.5 5255.7 5300.7 5058.5 4657.9 4276
NaN 10738.1 14239.2 17012.4 18513.1 17824.5 16368.4 15000.2
NaN 5052.2 15067 25199.9 32596 31827.7 28772.6 25780.1
NaN 10893.1 18710.5 25678 30153.1 29133.8 26364.8 23670.5
NaN 7507.6 13378.8 19232.4 23495.4 23001 21290.1 19732.6
NaN 0 0 0 7040.4 8075.5 7920.1 7907.9
527 531 535 539 543 547 551 555
0 0 0 0 0 0 0 0
10.1 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0
125.6 107.4 89.8 76.7 65.4 58.7 50 44.9
0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0
669.7 592.5 526.3 475.1 429.1 384.4 337.1 293.8
7649 6902.1 6240.9 5711.1 5208.8 4708.1 4164.1 3685.7
6624.4 5981.1 5413.6 4965.5 4540.5 4111.8 3642.2 3225.4
14764.3 13410.5 12205.4 11242.8 10306.8 9350.8 8304.8 7375.9
16163.3 15415 14731.1 14088.1 13311.5 12379 11252.2 10195.3
0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0
3950.2 3694.9 3475.4 3285.3 3070.8 2830.3 2546.9 2290.7
13815.8 12868.9 12023 11305.1 10541.5 9702.3 8734.5 7845.2
23095.3 20881 18908.8 17353.4 15868.1 14369.5 12739.2 11293.1
21253.8 19271.3 17506.4 16107.1 14757 13388.3 11880.8 10546.6
18434.9 17431.4 16527.9 15717.4 14790.4 13715.4 12421.4 11217.5
8015.6 8193.9 8343.6 8340.2 8153.6 7789.8 7260.7 6724.6
559 563 567 571 575 579 583 587
0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0
39.2 31.1 26.3 18.6 15.6 12.7 11.1 9.3
0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0
254.1 217 186.5 160.3 139.5 122.5 106.9 94.7
3242.5 2813.5 2471.3 2154.5 1906.7 1692.9 1492 1332.1
2840.2 2469.3 2173.3 1896.6 1678.7 1493.9 1318 1178.9
6513.8 5678.6 5014.1 4394.5 3906.8 3484.3 3084.5 2764.4
9195.4 8218.2 7443.9 6723.5 6151.9 5651.5 5158.6 4740
0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0
2046.8 1807.7 1618.7 1436 1297.2 1171.4 1050.2 951.4
7002.5 6176.3 5516.8 4894.8 4405.6 3977.5 3563.2 3220.6
9947.5 8654.4 7626.2 6661.7 5907.7 5252.3 4638.2 4142.6
9302.3 8103 7149.3 6253.7 5553.9 4947 4372.4 3910.9
10065.3 8933.7 8035 7187.6 6520 5929.9 5350.4 4865
6194.3 5664.2 5245.7 4848.3 4537.1 4260.5 3978 3730.7
591 595 599 603 607 611 615 619
0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0
7.6 7.4 6.7 6.3 5.7 4.2 3.3 3.7
0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0
83 73.5 64.3 55.3 48.2 42.6 37.3 33.8
1180 1055 934 832.6 734.6 656.7 580.1 513.8
1044.5 934.3 827.5 739 652.5 583.7 515.1 455.9
2456.3 2203.1 1954.2 1748.2 1549.5 1385.7 1225.7 1084.1
4317.1 3953.6 3581.6 3263.6 2940.1 2668.4 2401.9 2161.9
0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0
852 771.7 690.6 624.6 558.7 501.2 445.1 395.2
2882.7 2604.8 2330.1 2099.6 1872 1678.9 1490.4 1328.4
3667.8 3284.6 2909.6 2605.2 2309.5 2066.2 1829.1 1616.9
3469.9 3110 2756 2467.7 2186.2 1955 1729.4 1524.8
4379.9 3972.2 3560.5 3216.1 2871.3 2584.3 2304.3 2057.1
3464.8 3224.9 2965.6 2735.9 2493.1 2281.8 2070.2 1878.7
623 627 631 635 639 643 647 651
0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0
3 2.6 1.2 0 0 0 0 0.2
0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0
30.5 26.8 24.5 21.2 19 17.4 16.5 15.4
460.1 404.7 360.8 317.2 278.8 252 225.2 202.8
407.8 361.6 323.7 286.9 254.8 228.3 203 180.4
970.3 859.5 769.6 683.2 607.2 543.9 487.1 433.9
1964.3 1771 1609 1450.3 1307.5 1190.5 1077.1 971.9
0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0
354.2 315 283 253.5 226.3 206 183.5 165.5
1194.6 1066.5 958.6 853.3 756.1 678.1 605.3 542.9
1443.1 1279.8 1143.7 1013.8 896.9 803.9 718 642.2
1362.7 1207.1 1081.9 959.4 848.2 760.7 676.7 607.3
1856.5 1661 1498 1337.9 1193 1078.6 969 871.2
1721.8 1566.4 1431.5 1296.8 1176.6 1075.8 982.2 894.6
655 659 663 667 671 675 679 683
0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0
1 1.2 1.3 0.3 0 0 0 0
0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0
13.4 11.7 9.8 8.5 7.9 7.1 7 6.5
181.2 164.6 147.1 131.7 118 105.8 97 86.9
159.6 145.3 130.5 116.2 104.6 93.8 84 77.9
390.1 351.9 313.5 281.3 255.4 232 210.6 190.2
881 806.4 730.3 662.9 601.4 548.5 502.3 459.9
0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0
0 0 0 0 0 0 2 1.8
0 0 0 0 0 0 0 0
149.3 136.2 122.1 108.1 96.6 87.3 79.6 71.5
490.1 447.2 401.6 361.8 324.7 293.1 267.7 242.1
575.9 519.7 463.3 413.6 371.7 333.7 303.4 273.9
544.8 494.2 440.3 392.7 350.6 314 285.1 259.2
784 711.6 642.4 581.2 524.5 474.1 430.7 391.4
813.2 747.4 679.1 619.7 563.5 512.4 470.8 429.4
687 691 695 699 703 707 711 715
0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0
0 0 0 0 0 0.1 0 0
0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0
0.5 0 1.1 1.3 0.2 0.1 0 0.5
0 0.4 0.7 1.2 0.7 0.5 0.5 0.1
6.8 6.1 5.8 6 4.9 3.9 2.4 0.8
76.9 68.2 60.7 56.1 49.6 45.5 38.1 33.5
68.2 60.6 53.9 48 43 40.1 36.8 35.6
169.9 152.9 134.7 117.2 103.4 90.2 82.1 75.5
418.2 382.6 347.3 310.6 280.7 255.9 237.1 217.7
0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0
62.6 55.2 48.9 43 41.9 34.7 31.4 26.7
216.3 192.3 172.7 152.2 137.8 123.5 112.5 102.2
246.5 219.9 197.7 176.6 162.2 146.1 132.5 118.5
231.5 210.3 184.8 164.2 146.7 130.1 115.7 100.9
354.6 320.8 285.8 255.2 227.3 205.1 185.9 167
388.7 351.8 316.6 285.1 261.1 238.1 218.4 198.3
719 723 727 731 735 739 743 747
0 0 0 0 0 0 0 0
0 0 1.4 1.3 1.4 0 0 0
0 0 0 0 0 0 0 0
0 0 2.2 1.6 1.1 0 0 0
0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0
0 0 1.8 0 0.8 0 0 0
0 0 0 0 0 0 0 0
1.5 0.4 0.9 0 0 0 0 0
0.3 0.8 2.3 2.1 3.5 3.3 0.2 0
1.4 3.5 5.4 4.2 1.7 0 0 0.1
29.3 25.2 24.9 22.5 24.5 23.8 22.1 14.7
31.9 25.9 20.2 15.9 13.8 13.6 12.6 7.8
67.7 61.7 50.4 47.4 48.5 49.3 50.2 34.5
200.7 182.6 169.1 158.6 152.6 140.9 130 88.7
0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0
0 0 0 0 0 0.5 0 0
0 0 0 0 0 0 0 0.3
0 0 0 0 0 0.4 1.7 2.3
0 0 0 0 0 0.7 2.1 2.2
22.4 21.3 20.2 23.2 21.4 19.9 18.2 12.9
90 82.2 76.7 73.9 66.8 58.6 52.7 35.9
107.4 95.1 86.9 78.9 73.3 73.9 73.5 52
86.2 75.2 69.2 68.9 62.7 58.2 52.9 35.4
146.9 133.6 117.8 113 100.2 95 88.4 61.5
178.5 163.6 150.3 139.9 127.4 118.5 109.3 76.1
751 raw 395 raw 399 raw 403 raw 407 raw 411 raw 415 raw 419
0 293 302 303 310 329 309 309
0 290 306 297 309 328 314 308
0 290 311 306 315 347 311 308
0 292 304 299 306 326 309 304
0 297 306 303 320 341 320 327
0 293 305 307 316 350 323 331
0 294 311 299 310 352 320 316
0 289 311 303 318 347 325 324
0 293 302 304 312 342 326 354
0 290 294 296 301 315 316 353
0 285 296 296 300 322 307 315
0 280 284 282 284 290 287 286
0 281 282 283 285 292 287 287
0 278 279 279 280 285 280 283
0 279 282 281 283 289 291 304
0 282 288 285 292 298 290 284
0 278 291 285 294 304 290 292
0 284 286 283 287 299 288 288
0 282 285 286 292 308 292 296
0 280 285 279 286 296 286 286
0 282 288 286 290 301 294 294
0 275 280 277 277 288 282 278
0 275 275 275 277 279 276 276
0 274 276 275 276 277 279 283
0 272 274 275 273 277 276 275
0 274 275 275 275 278 276 278
0 279 283 279 282 287 286 296
raw 423 raw 427 raw 431 raw 435 raw 439 raw 443 raw 447 raw 451
363 398 492 681 1027 1535 2227 2989
359 404 514 731 1106 1678 2428 3286
352 379 436 566 812 1172 1683 2251
345 364 415 550 771 1087 1537 2036
400 473 636 1020 1609 2564 3745 4095
415 526 733 1197 1957 3135 4095 4095
391 446 589 878 1385 2150 3115 4095
401 485 635 997 1590 2506 3673 4095
466 621 948 1691 2801 4095 4095 4095
457 642 990 1805 3072 4095 4095 4095
384 463 622 974 1541 2472 3610 4095
308 323 359 437 567 770 1047 1371
309 330 378 470 629 883 1217 1606
294 305 328 382 464 592 766 969
344 422 587 933 1512 2440 3612 4095
308 321 357 426 546 725 966 1240
328 350 406 534 746 1078 1514 2026
316 340 378 486 653 924 1254 1658
326 364 432 582 847 1226 1760 2350
309 321 352 414 540 710 928 1202
334 366 444 601 874 1273 1816 2434
287 291 301 325 364 417 488 578
282 285 290 305 332 372 423 487
294 316 358 445 581 792 1084 1405
278 278 281 290 303 322 344 377
286 299 327 381 473 617 820 1061
331 391 516 788 1244 1986 2996 4093
3891 4095 4095 3796 3145 2498 2080 1718
4095 4095 4095 4095 3457 2723 2241 1840
2926 3249 3152 2864 2377 1896 1616 1402
2670 2970 2867 2627 2177 1743 1497 1318
4095 4095 4095 4095 4095 4095 3413 2669
4095 4095 4095 4095 4095 4095 4095 3183
4095 4095 4095 4095 4095 3543 2849 2290
4095 4095 4095 4095 4095 4095 3625 2839
4095 4095 4095 4095 4095 4095 4095 4095
4095 4095 4095 4095 4095 4095 4095 4095
4095 4095 4095 4095 4095 4095 3685 2949
1786 1977 1970 1829 1575 1326 1213 1183
2102 2331 2291 2109 1790 1493 1327 1241
1247 1380 1368 1285 1128 979 939 989
4095 4095 4095 4095 4095 4095 3775 3128
1595 1747 1724 1575 1341 1113 1013 981
2649 2923 2835 2589 2170 1738 1502 1324
2175 2368 2323 2118 1789 1462 1241 1148
3062 3386 3278 3004 2477 1985 1676 1450
1515 1685 1620 1494 1252 1046 962 941
3161 3446 3309 3008 2505 1977 1710 1490
685 731 719 686 625 589 626 753
562 599 594 572 541 541 605 768
1812 1987 1924 1771 1489 1245 1120 1079
410 429 425 420 410 426 491 637
1402 1582 1575 1476 1274 1105 1067 1118
4095 4095 4095 4095 4095 4020 3394 2897
1613 1849 2227 2691 3135 3418 3419 3219
1686 1856 2205 2678 3099 3401 3351 3192
1391 1672 2092 2560 3024 3299 3246 3100
1312 1658 2125 2663 3133 3454 3429 3241
2225 2138 2147 2296 2568 2789 2772 2627
2573 2434 2426 2546 2820 3045 3020 2864
2010 2055 2269 2604 2983 3212 3238 3045
2362 2135 2007 1983 2113 2297 2274 2164
3681 3057 2547 2218 2232 2391 2404 2249
3676 2802 2003 1354 1111 1150 1145 1096
2517 2508 2605 2846 3195 3455 3392 3252
1308 1715 2221 2771 3251 3510 3488 3313
1342 1699 2152 2664 3132 3360 3353 3177
1182 1621 2158 2706 3182 3427 3397 3240
2784 2795 2852 2900 3065 3215 3172 3054
1127 1500 2044 2641 3138 3415 3411 3224
1372 1678 2118 2668 3166 3434 3420 3232
1216 1589 2072 2609 3094 3362 3352 3174
1436 1742 2180 2678 3164 3440 3398 3220
1084 1482 2020 2612 3092 3366 3381 3192
1492 1780 2205 2690 3136 3398 3391 3208
1001 1475 2047 2634 3136 3382 3367 3208
1062 1576 2175 2777 3280 3511 3498 3348
1216 1597 2106 2635 3119 3370 3357 3192
905 1384 1974 2592 3104 3356 3347 3183
1359 1815 2342 2852 3279 3497 3462 3320
2730 2893 3044 3167 3348 3486 3437 3336
2863 2564 2295 2080 1886 1749 1642 1497
2854 2494 2213 2004 1801 1667 1524 1389
2778 2479 2225 2046 1851 1719 1626 1471
2893 2567 2287 2081 1892 1726 1617 1477
2335 2091 1866 1728 1531 1435 1367 1240
2529 2267 2032 1881 1669 1554 1479 1348
2715 2406 2132 1985 1770 1653 1539 1409
1915 1762 1598 1489 1365 1268 1230 1121
2025 1822 1655 1531 1384 1305 1244 1152
980 898 826 785 726 692 668 633
2883 2557 2292 2093 1879 1758 1617 1493
2980 2671 2433 2243 2078 1945 1813 1674
2861 2573 2326 2151 1990 1863 1757 1640
2927 2648 2415 2236 2087 1971 1861 1720
2826 2633 2503 2413 2348 2281 2192 2072
2897 2576 2321 2102 1930 1795 1679 1544
2906 2586 2314 2100 1909 1792 1673 1540
2836 2531 2279 2084 1896 1758 1638 1503
2890 2596 2332 2132 1938 1808 1683 1554
2856 2559 2298 2113 1915 1802 1682 1556
2892 2608 2359 2180 1993 1864 1775 1625
2906 2634 2408 2238 2111 1993 1887 1745
3051 2791 2585 2421 2288 2185 2076 1939
2887 2602 2359 2176 2029 1905 1787 1661
2880 2597 2362 2188 2037 1919 1810 1674
3065 2821 2637 2514 2417 2328 2237 2093
3139 3004 2910 2878 2872 2822 2752 2624
1364 1226 1097 972 873 800 712 666
1284 1165 1030 902 800 731 664 626
1364 1250 1090 1004 891 816 721 700
1364 1225 1081 979 863 793 693 658
1156 1068 931 840 760 711 630 599
1233 1121 995 903 808 747 667 635
1312 1182 1047 933 853 781 692 661
1062 978 863 803 721 688 620 582
1052 971 878 799 712 681 603 585
594 566 517 496 456 444 414 409
1365 1249 1089 977 871 789 726 672
1551 1386 1246 1109 997 902 825 753
1490 1350 1212 1087 973 879 805 747
1569 1428 1284 1152 1034 938 859 790
1916 1760 1595 1451 1313 1215 1127 1053
1401 1266 1140 1015 910 819 746 689
1382 1262 1117 997 894 823 742 688
1384 1240 1102 994 890 804 720 680
1401 1274 1142 1028 908 816 750 688
1418 1284 1143 1008 906 834 754 698
1474 1333 1208 1076 976 892 798 737
1596 1459 1306 1179 1049 962 877 808
1787 1622 1461 1310 1180 1066 981 906
1516 1369 1232 1103 992 900 827 757
1533 1385 1244 1115 1000 909 832 766
1931 1758 1594 1437 1295 1179 1088 1003
2446 2260 2063 1880 1722 1587 1482 1385
633 583 539 511 479 453 437 417
591 543 500 477 450 424 408 392
652 595 543 527 489 461 445 421
622 577 531 507 467 452 430 408
581 540 490 470 445 421 417 396
604 558 523 495 464 451 427 400
619 564 530 514 463 460 433 411
572 535 495 479 451 432 419 404
555 516 486 464 439 419 422 393
399 382 367 356 344 342 333 328
633 576 539 508 470 454 431 410
706 654 608 573 534 502 475 450
692 644 597 562 527 497 467 445
734 682 636 597 557 526 489 468
992 922 857 807 749 701 648 613
637 596 555 521 486 461 440 418
643 584 546 517 481 460 438 410
631 584 548 511 484 452 432 411
644 593 558 526 484 460 436 416
651 602 562 531 498 472 448 421
688 637 591 557 522 495 466 440
753 700 649 609 564 533 502 475
841 782 720 677 628 589 547 512
700 653 607 570 532 501 473 451
711 658 613 577 539 509 476 451
932 864 804 749 693 647 601 562
1316 1220 1141 1075 989 924 851 791
395 381 370 361 346 343 349 334
370 361 363 346 335 335 337 324
407 386 384 377 351 350 358 340
397 380 375 353 335 350 339 328
379 365 371 354 336 334 348 337
388 370 363 356 333 344 359 340
390 381 372 359 340 345 354 343
384 376 369 363 333 343 347 341
375 369 357 356 333 334 340 326
322 315 319 313 300 306 315 300
389 383 367 359 340 341 332 332
428 405 392 378 363 355 347 332
422 400 390 375 363 354 344 335
442 418 402 386 372 360 350 340
572 535 511 486 460 439 421 404
398 381 373 367 345 340 337 328
398 381 375 362 348 342 338 327
392 378 368 351 338 334 333 319
400 382 374 358 342 341 338 325
402 385 377 362 346 340 334 328
417 407 388 374 360 348 348 334
443 423 406 390 376 361 355 341
487 455 439 417 398 382 370 357
426 404 388 373 361 349 340 331
429 408 390 377 362 351 341 333
525 493 471 445 427 405 391 375
732 680 648 604 563 527 500 480
343 330 337 318 314 313 310 305
334 328 333 314 304 312 304 298
352 338 349 325 320 322 314 303
337 327 332 319 313 309 309 307
335 336 341 324 315 316 306 302
349 332 347 322 318 323 312 305
349 336 348 325 319 316 316 308
347 339 345 322 315 315 312 305
336 334 336 320 317 314 307 302
306 302 305 298 296 295 292 287
326 327 331 314 308 309 308 302
332 322 321 313 308 304 299 299
333 323 320 313 306 306 300 297
332 326 323 314 310 306 301 299
390 374 366 353 346 336 328 325
323 320 321 309 301 299 298 295
332 317 320 311 306 302 301 300
325 316 316 306 302 300 296 292
326 318 324 310 306 302 302 297
324 318 318 309 302 304 298 294
332 326 328 314 308 303 300 300
336 327 322 317 309 305 305 297
348 338 332 324 316 312 309 304
323 317 311 306 302 298 295 293
325 318 313 306 303 300 295 293
363 351 342 333 326 320 313 308
458 436 417 397 388 376 361 350
315 310 311 311 302 304 306 301
318 302 304 306 299 303 310 298
325 305 325 322 305 310 319 306
314 303 308 304 297 303 305 306
319 308 317 312 302 316 313 308
323 313 312 312 297 313 315 308
315 305 313 313 299 311 306 306
323 304 318 313 304 310 311 305
316 309 312 310 303 306 310 299
300 290 294 295 291 290 294 290
306 302 293 302 297 299 297 298
300 294 294 294 291 288 290 287
297 296 294 293 289 290 288 284
298 295 293 293 290 288 286 285
320 314 311 307 301 301 298 296
299 297 292 295 290 291 289 288
302 294 300 294 292 294 293 293
294 292 292 292 288 290 290 287
302 295 294 296 288 290 296 290
298 294 298 293 290 292 290 286
302 296 299 298 289 291 293 290
300 293 295 292 290 288 285 284
302 296 295 293 288 288 286 284
291 289 288 286 283 281 282 280
292 288 287 286 283 281 282 280
306 301 301 296 292 290 289 286
345 337 331 324 316 312 307 302
309 306 307 296 311 299 303 301
311 304 306 293 307 299 297 297
317 307 310 297 317 300 309 308
305 302 301 292 312 294 299 293
314 311 305 295 316 304 304 309
321 313 313 300 316 308 302 306
313 310 310 307 316 300 301 311
314 310 311 300 320 302 308 308
308 306 307 300 309 306 304 302
295 288 291 288 296 290 286 294
302 295 296 294 300 291 290 296
287 286 285 284 284 282 286 285
287 288 285 285 287 284 285 282
285 283 283 282 283 279 281 280
292 292 291 288 289 286 286 285
289 286 288 287 287 285 288 284
294 292 294 286 296 288 286 288
290 288 293 279 290 286 285 284
290 288 291 280 290 290 289 286
290 289 292 282 288 287 284 285
295 291 292 281 293 290 286 288
285 284 283 280 284 281 282 278
284 283 283 278 281 281 280 279
279 278 279 278 277 277 276 276
279 278 277 277 278 276 276 276
286 285 281 281 280 278 280 277
301 298 296 292 293 286 287 285
raw 743 raw 747 raw 751
299 308 290
296 308 289
300 313 295
293 310 289
303 319 296
303 323 297
302 320 292
301 316 293
294 311 285
289 296 280
292 300 280
282 285 281
282 284 279
280 282 279
284 285 281
283 290 282
290 296 286
282 291 275
285 296 280
284 290 279
286 294 281
280 284 276
277 279 275
275 276 274
274 276 274
277 279 274
282 286 282
Nanodrop 3300 ( fluorescencia)
eppendorf cc fluoresceina RFU

1 19768.2
2 20984.9
3 9534.7
4 11064.7
5 958.2
6 0
Nanodrop 1000 (Uv- Vis)

eppendorf cc fluoresceina Abs. 230 Abs. 490
1 0.887 1.820
2 0.986 1.254
3 0.622 0.767
4 0.370 0.437
5 0.249 0.242
6 0.137 0.124
7 0.054 0.040
8 0.028 0.019
9 0.003 0.002
10 0 0
N Dilucin Concentracin terica de
fluorescena (M)
1 0.02
2
4 x 10-3
3
8 x 10-4
4
1,6 x 10-4
5
3,2 x 10-5
6
6,4 x 10-6
7
1,28 x 10-6
8 2,56 x10-7
9
5,12 x 10-8
10
1,02 x 10-8
Ndilucion cc fluorescena RFU Nanodrop 3300
1 0.2 19768.2
2 0.04 20984.9
Grfico de Abs 490 nm vs concentracin de fluorescena
3 0.008
2.000 9534.7
4 0.0016 11064.7
1.800 f(x) = 8.5678909821x + 0.2563427474
5 0.00032 R = 0.7373488859
958.2
1.600
6 0.000064 0
1.400
Absorbancia 1.200
1.000
Ndilucion cc fluorescena 0.800 Abs. 230 Abs. 490 Nanodrop 1000

1 0.600
0.2 0.887 1.820
2 0.400
0.04 0.986 1.254
3 0.008
0.200 0.622 0.767
4 0.0016
0.000 0.370 0.437
0 0.05 0.1 0.15 0.2 0.25
5 0.00032 0.249 0.242
6 0.000064 0.137 0.124 Concentracin (M)
7 0.0000128 0.054 0.040
8 0.00000256 0.028 0.019
9 0.000000512 0.003 0.002
10 1.024E-07 0 0
Grfico de RFU () vs Concentracin de fluorescena
uorescena
25000
20000 f(x) = 73315.616021951x + 7330.4948407281

R = 0.4217461245
RFU (F) 15000
10000
5000
0.2 0.25 0
0 0.05 0.1 0.15 0.2 0.25
Concentracin (M)
Grfico de Abs 230nm vs concentracin de fluorescena
1.200
1.000
f(x) = 3.9274508027x + 0.23541374
R = 0.4355264035
0.800
Absorbancia
0.600
0.400
0.200
0.000
0 0.05 0.1 0.15 0.2 0.25
Concentracin (M)
escena
0.2 0.25
NADH RFU
0.1 13
0.2 24.6
0.3 49.9
0.5 59.7
0.6 71.2
16
1200
0.7 83.5
0.8 95.1 14 f(x)f(x)
= 349933.554817276x
= 133.25x + 326.5 + 0.0214285714
1000 R =R0.9997603331
= 0.9998775182
x 42.3
12
800
0 0 10
8.60E-06 3.02
1.72E-05 5.98 8
600
2.58E-05 9.18 6
3.44E-05 12.13 400
4.30E-05 14.96 4
200
2
0.00E+00 323
2.00E+00 597 0 0
0.00E+00
0 2.00E+00
0.00001 4.00E+00
0.00002
6.00E+00 0.00003
8.00E+00
4.00E+00 862
6.00E+00 1123
100
90 f(x) = 112.2020547945x + 5.4219178082
R = 0.9727971525
80
70
817276x
326.5 + 0.0214285714 60
1
182 50
40
30
Column B20
Linear (Column
10 B)
0
0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9
4.00E+00
0.00002
6.00E+00 0.00003
8.00E+00 0.00004 0.00005
0.8 0.9

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Datos TP2 para Alumnos

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Method: TAI 2015

eppendorf cc fluoresceina RFU

Nanodrop 1000 (Uv- Vis)

Ndilucion cc fluorescena 0.800 Abs. 230 Abs. 490 Nanodrop 1000

20000 f(x) = 73315.616021951x + 7330.4948407281

RFU (F) 15000

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