1 atgaagtttg gtaacattta tttttcgtat cagccaccag gcgaaactca taagcagata

61 atggatcgtt ttgtgagact agggagcgtt tctgaagagc ttggttttga tacatattgg

121 acgttagaac atcattttac tgaattcggt ttaactggga gcttgtttgt ttccgctgct
181 aatttattag gaagaacaaa aacattgaaa gtaggaacta tgggggtcgt tgtacctact
241 gctcaccccg tacggcaagc agaggatgta ttgctgttag atcaactttc taaaggtcga
301 tttaattttg gtgttgttag aggcctatat cacaaggatt ttcgggtctt tggggtcaat
361 atggaagaat ctcgtgaaat aacacaagat tttcatcaga tgataatgaa gagctttgat
421 gaaggagcga tccatagtaa tagtaaacat attgattttc cagacgttaa tgtttatcca
481 acaaagtact ctaaagaaat taggacttgt atgacagcgg agtcagctag tactactgag
541 tggctagcaa ggcaagggat gccaatggta cttagttgga ttataggaac aaatgagaaa
601 aaaacacaaa tggagctata taatgaaata gcgacagaac atggccacga cataagtaag
661 attgatcatt gtttaaccta tatttgttcc gttcacgaag atggggatag agctcgtgag
721 gtatgtcgga agttcttgat cagtttttac gattcttatg taaatgcaac caacattttt
781 aatgatagtg atcaaactcg aggttacgat tatcacaaag gccagtggag agattttgta
841 ttacaaggtc atactgatac gaatcgacgt gttgattata gtcatggact taatcctgtt
901 ggaactcctg cagagtgcct tgagattatt cagcgtgata ttgatgcgac tggtattact
961 aacgtcacct gtggttttga ggctaatggc actgaagaag aaattattgc ttcgatggaa
1021 ctcttcatga ccaaggttgc ccctttctta aaagaacctc aaaaataa

5---------------------------3

Forward Primer: gttgatggtc cgctttgagt a

Reverse Primer: atcgtaaggtcgagtttga

Computer generated Primers.

Left Primer
Right Primer

1 atgaagtttg gtaacattta tttttcgtat cagccaccag gcgaaactca taagcagata

61 atggatcgtt ttgtgagact agggagcgtt tctgaagagc ttggttttga tacatattgg
121 acgttagaac atcattttac tgaattcggt ttaactggga gcttgtttgt ttccgctgct
181 aatttattag gaagaacaaa aacattgaaa gtaggaacta tgggggtcgt tgtacctact
241 gctcaccccg tacggcaagc agaggatgta ttgctgttag atcaactttc taaaggtcga
301 tttaattttg gtgttgttag aggcctatat cacaaggatt ttcgggtctt tggggtcaat
361 atggaagaat ctcgtgaaat aacacaagat tttcatcaga tgataatgaa gagctttgat
421 gaaggagcga tccatagtaa tagtaaacat attgattttc cagacgttaa tgtttatcca
481 acaaagtact ctaaagaaat taggacttgt atgacagcgg agtcagctag tactactgag
 541 tggctagcaa ggcaagggat gccaatggta cttagttgga ttataggaac aaatgagaaa
601 aaaacacaaa tggagctata taatgaaata gcgacagaac atggccacga cataagtaag
661 attgatcatt gtttaaccta tatttgttcc gttcacgaag atggggatag agctcgtgag
721 gtatgtcgga agttcttgat cagtttttac gattcttatg taaatgcaac caacattttt
781 aatgatagtg atcaaactcg aggttacgat tatcacaaag gccagtggag agattttgta
841 ttacaaggtc atactgatac gaatcgacgt gttgattata gtcatggact taatcctgtt
901 ggaactcctg cagagtgcct tgagattatt cagcgtgata ttgatgcgac tggtattact
961 aacgtcacct gtggttttga ggctaatggc actgaagaag aaattattgc ttcgatggaa
1021 ctcttcatga ccaaggttgc ccctttctta aaagaacctc aaaaataa

Comparison.
Both primer sets met the critera of having a G/C content between 40-60%,
the ending base pairs at the 3 terminal end are complimentary, the primer set is
non-complementary to each other, but both the computer and I included the T-base
pair at the 3 terminal end. We both utilized the method of having the forward and
reverse primer contain a high percentage of two specific bases. I have chosen to
have a high percentage of G and T base pairs in both primers while the computer
generated one chosen A and G so they wont be complementary to each other. We
both chosen to be on the lower side of primer length hovering at around twenty
nucleotides. My forward primer overlaps heavily with the computer generated one,
but my reverse did not. The parameter optimization at 600 was inputted causing
the program to choose a primer in the 600 region while I chose a primer in the 800
region for the reverse. Both sets of primers met all criteria except for avoiding T-
base pairs at the end.
.