Calculo de Acido Grasos

Acido Graso C13: 0

cantidad de la solucion (g)
densidad de cloroformo(g/ml)
concentracion (mg/L)

Dilucion de la muestra
masa de la muestra (g)
1.1422
Volumen metilacion (ml)
20
Volumen alicuota (ml)
5
Volumen de enrace (ml)
20
Factor de dilucion ( L )
0.08

C11:0(mg/L)
C13:0(mg/L)

Ester Metilico de C11:0

Volumen de la solucion (ml)
concentracion (mg/L)

Concentracion Teorica de patron interno en la Ultima Dilucion

Ester Metilico de Acidos Grasos
40.26
84.09067765
Concentracion Experimental de patron interno en la Ultima Dilucion

Nombre
C11:0
C13:0

Ester Metilico de Acidos Grasos

estandar
muestra
[mg/L]
Area
Area
78.1
1745747
569319
43.400
1027770
845275
Concentracion Experimental en la Ultima Dilucion
ester metilico de acidos grasos
estandar
muestra

Nombre
C14:0
C14:1cis 9
C16:0
C16:1cis 9
C18:0
C18:1cis 9
C18:1cis 11
C18:2cis 9 12
C20.0
C18:3 cis 6 9 12
C20:1 cis 11
C18:3 cis 9 12 15
C18:4 cis 6 9 12 15
C20:2 cis 11 14
C22:0
C20:3 cis 8 11 14
C22:1 cis 13
C20:3 cis 11 14 17
C20:4 cis 5 8 11 14
C20:4 cis 8 11 14 17
C24:0

[mg/L]
31.640
5.270
84.370
26.370
42.180
68.550
21.090
10.550
5.270
6.410
47.460
10.550
0.000
9.950
5.270
5.440
15.820
5.270
15.820
0.000
5.270

Area
693399
112507
1870134
564700
916087
1539902
509172
230924
119392
160261
1018078
217077

Area
338827
31749
2678385
695116
811945
4005692
469547
3431763
22814
71203
157322
269593

201428
98720
106591
310833
100676
288175

188376
16318
100055
17183
6757
92311

836085

191789

C20:5 cis 5 8 11 14 17
C24:1 cis 15
C22:4 cis 7 10 13 16
C22:5 cis 4 7 10 13 16
C22:5 cis 7 10 13 16 19
C22:6 cis 4 7 10 13 16 19

52.730
5.270
5.300
4.500
8.320
63.280

47330
48205
71309
59119
89752
650302

8351
4611
20669
54056
490690

cido Graso C13: 0

3.1871
1.484
2940

er Metilico de C11:0
1
805.20

Dilucion
Acido Graso
37.44091389
78.92582547
% Recuperacion
C13:0

a Dilucion

uestra

Acido Graso
muestra
[mg/L]
[mg/L]
40.26
37.44091389
56.420922 52.9555473

67.09533538

Factor de
Correccion
1.58069603
Nombre
C11:0
C13:0

n
Acido Graso
muestra

uestra
[mg/L]
36.423973
3.5036213
284.67174
76.472566
88.07497
420.09544
45.819165
369.36583
2.372427
6.7094127
17.277948
30.867631
0
21.922236
2.0522413
12.03021
2.0603186
0.833287
11.938772
0
2.8479995

[mg/L]
34.31625302
3.29917958
269.9039699
72.47843194
83.93824911
400.2298878
43.65149323
351.7799628
2.270500411
6.387546287
16.53152494
29.38784205
0
20.96862234
1.971045252
11.50395832
1.978394368
0.796819424
11.41322488
0
2.743653756

C14:0
C14:1cis 9
C16:0
C16:1cis 9
C18:0
C18:1cis 9
C18:1cis 11
C18:2cis 9 12
C20:0
C18:3 cis 6 9 12
C20:1 cis 11
C18.3 cis 9 12 15
C18:4 cis 6 9 12 15
C20:2 cis 11 14
C22:0
C20:3 cis 8 11 14
C22:1 cis 13
C20:3 cis 11 14 17
C20:4 cis 5 8 11 14
C20:4 cis 8 11 14 17
C24:0

0
2.1508645
0.8073887
3.7064738
11.805357
112.49018

0
2.071606437
0.774701772
3.555574924
11.32461776
107.8856098

C20:5 cis 5 8 11 14 17
C24:1 cis 15
C22:4 cis 7 10 13 16
C22:5 cis 4 7 10 13 16
C22:5 cis 7 10 13 16 19
C22:6 cis 4 7 10 13 16 19

MASA MOLECULAR
Ester Metilico de Acidos Grasos
200.3213
228.375

acido graso
186.2944
214.3482

MASA MOLECULAR
Ester Metilico de Acidos Grasos
242.402
240.3861
270.45
268.43
298.5
296.48
296.4936
294.47
326.56
292.46
324.53
292.45
290.45
322.53
354.61
320.5
352.59
320.5156
318.49
318.49976
382.66

acido graso
228.3751
226.3592
256.42
254.41
284.48
282.46
282.4667
280.45
312.53
278.43
310.51
278.43
276.41
308.5
340.58
306.48
338.57
306.4887
304.47
304.4728
368.64

316.48
380.6549
346.55
344.5376
344.53
342.51

302.45
366.628
332.52
330.5107
330.5
328.49

Nombre
C14:0
C14:1cis 9
C16:0
C16:1cis 9
C18:0
C18:1cis 9
C18:1cis 11
C18:2cis 9 12
C20:0
C18:3 cis 6 9 12
C20:1 cis 11
C18:3 cis 9 12 15
C18:4 cis 6 9 12 15
C20:2 cis 11 14
C22:0
C20:3 cis 8 11 14
C22:1 cis 13
C20:3 cis 11 14 17
C20:4 cis 5 8 11 14
C20:4 cis 8 11 14 17
C24:0
C20:5 cis 5 8 11 14 17
C24:1 cis 15
C22:4 cis 7 10 13 16
C22:5 cis 4 7 10 13 16
C22:5 cis 7 10 13 16 19
C22:6 cis 4 7 10 13 16 19
TOTAL
SFA
MUFA
PUFA n-6
lcPUFA n-6
PUFA n-3
lcPUFA n-3
n-6/n-3

% Humedad
60.00
Esteres metilicos de acidos grasos
mg /100 g
mg /100 g MS
255.11
637.79
24.54
61.35
1993.85
4984.62
535.62
1339.04
616.88
1542.20
2942.36
7355.90
320.92
802.30
2587.05
6467.62
16.62
41.54
46.99
117.48
121.02
302.54
216.20
540.49
0.00
0.00
153.54
383.86
14.37
35.93
84.26
210.65
14.43
36.08
5.84
14.59
83.62
209.05
0.00
0.00
19.95
49.87
0.00
0.00
15.06
37.66
5.65
14.14
25.96
64.90
82.69
206.71
787.88
1969.71
10970.41
27426.02
2916.78
3973.94
2987.080
353.039
1092.603
876.406
2.734

7291.95
9934.86
7467.699
882.596
2731.509
2191.014
2.734

Acidos Grasos
mg /100g

240.35
23.11
1890.41
507.64
587.91
2803.22
305.74
2463.88
15.90
44.74
115.79
205.83
0.00
146.86
13.81
80.57
13.86
5.58
79.94
0.00
19.22
0.00
14.51
5.43
24.90
79.32
755.63
10444.14
2767.60
3783.86
2846.322
337.707
1046.366
840.533
2.720

600.88
57.77
4726.04
1269.10
1469.76
7008.05
764.34
6159.69
39.76
111.85
289.47
514.58
0.00
367.16
34.51
201.44
34.64
13.95
199.85
0.00
48.04
0.00
36.27
13.57
62.26
198.29
1889.08
26110.36

% de esteres metilicos de
acidos grasos identificados
2.33
0.22
18.17
4.88
5.62
26.82
2.93
23.58
0.15
0.43
1.10
1.97
0.00
1.40
0.13
0.77
0.13
0.05
0.76
0.00
0.18
0.00
0.14
0.05
0.24
0.75
7.18
100.00

% de acidos grasos
identificados
2.30
0.22
18.10
4.86
5.63
26.84
2.93
23.59
0.15
0.43
1.11
1.97
0.00
1.41
0.13
0.77
0.13
0.05
0.77
0.00
0.18
0.00
0.14
0.05
0.24
0.76
7.23
100.00

6918.99
9459.65
7115.804
844.267
2615.915
2101.332
2.720

26.59
36.22
27.229
3.218
9.960
7.989
2.734

26.50
36.23
27.253
3.233
10.019
8.048
2.720

Acidos Grasos
mg /100 g MS