µ=Tasa de crecimiento o velocidad específica de crecimiento

DETERMINACION DE µmax ln X = µ t µmax=1.0411 h-1

tiempo (h) N - No. de m.o. (UFC/ml) ln X G=ln2/µ = 0.666 h =40 min
0 100 4.60517018598809
16
5.29831736654804

ln (UFC/ml)
1 200
14 f(x) = 1.04114082754797 x + 4.38384583454
2 500 6.21460809842219 R² = 0.998198607357343
12
3 2000 7.60090245954208
10
4 6000 8.69951474821019
8
5 15000 9.61580548008435
6
6 40000 10.5966347330961
4
7 100000 11.5129254649702
2
8 300000 12.6115377536383
0
9 1000000 13.8155105579643 0 2 4 6

10 3000000 14.9141228466324 Tiempo (h)

G=Tiempo de generación
G=ln2/µ

Tiempo D.O.(600 nm) µ=?

0 0.303 µ= 0.229 h-1
2 0.384 G=ln2/µ = 3.03 h = 182 min
4 0.492
6 0.765
8 1.51
10 3.01

Rendimientos

X S P
Tiempo (h) Biomasa (g/l) Azúcares totales (g/l) Ac. láctico (g/l)
0 0.32 19.62 0.3
2 0.62 19.35 0.78
4 1.4 15.89 1.77
6 2.31 11.21 4.8
8 2.72 7.82 6.36
10 2.73 7.09 6.78
12 2.97 6.85 7.11
26 2.79 5.64 7.61

Yx/s
Azúcares totales (Biomasa (g/l)
Biomasa (g/l)
3.5

3
f(x) = − 0.188826178164651 x + 4.23637188974304
 19.62 0.32 Biomasa (g/l)
19.35 0.62 3.5
15.89 1.4 3
11.21 2.31 f(x) = − 0.188826178164651 x + 4.23637188974304
2.5 R² = 0.980183252889812
7.82 2.72

Biomasa (g/l)
2
7.09 2.73
6.85 2.97 1.5

5.64 2.79 1

0.5

0
6 8 10 12 14 16 18 20
Azúcares totales (g/l)
YP/S
Azúcares totales (Ac. láctico (g/l)
19.62 0.3 YP/S= 0.5202 g ac. láctico/g azúcares totales
19.35 0.78
15.89 1.77 Ac. láctico (g/l)
11.21 4.8
8
7.82 6.36
7 f(x) = − 0.520164082020424 x + 10.5162170933061
7.09 6.78 R² = 0.993946516364304
6
6.85 7.11
Ac. láctico (g/l)

5
5.64 7.61
4
3
2
1
0
4 6 8 10 12 14 16 18 20
Azúcares totales (g/l)

Yp/X Yp/x= 2.6913 g acido láctico/g biomasa

Biomasa (g/l) Ac. láctico (g/l)
0.32 0.3
Ac. láctico (g/l)
0.62 0.78
8
1.4 1.77
7
2.31 4.8 f(x) = 2.69127121116351 x − 1.03927353284388
6 R² = 0.970249519474224
2.72 6.36
Ac. láctico (g/l)

5
2.73 6.78 4
2.97 7.11 3
2.79 7.61 2
1
0
0 0.5 1 1.5 2 2.5 3
Biomasa (g/l)
 3

Ac. lá
2
1
0
0 0.5 1 1.5 2 2.5 3
Biomasa (g/l)
de crecimiento
x=1.0411 h-1
2/µ = 0.666 h =40 min

4114082754797 x + 4.38384583454161
8198607357343

2 4 6 8 10 12
Tiempo (h)

Yx/s=0.1888 g biomasa/g azúcares totales

/l)

637188974304
/l)

637188974304

16 18 20 22
les (g/l)

tico/g azúcares totales

o (g/l)

10.5162170933061

14 16 18 20 22
otales (g/l)

o (g/l)

− 1.03927353284388

2 2.5 3 3.5
sa (g/l)
 2 2.5 3 3.5
sa (g/l)