Sol %T530 nm %T690 nm Conc.

M 50 90
5*10-4 M 0.0005
N 90 25
Mezcla 75 40

𝐴=−log⁡(%𝑇)

530 nm 690 nm
M AM = 0.301 AM = 0.046
N AN = 0.046 AN = 0.602
Mezcla Aλ 1 = 0.125 Aλ 2 = 0.398

530 𝜀_𝑀=𝐴_𝑀/𝐶_𝑀 = 602 𝜀_𝑁=𝐴_𝑁/𝐶_𝑁 = 92

690 𝜀_𝑀=𝐴_𝑀/𝐶_𝑀 = 92 𝜀_𝑁=𝐴_𝑁/𝐶_𝑁 = 1204

REEMPLAZANDO:

𝑨^𝝀𝟏= 〖𝜺 _𝑴 𝒃 〗 ^𝟏 𝑪_𝑴+ 〖𝜺 _𝑵 𝒃 〗 ^𝟏 𝑪_𝑵

𝑨^𝝀𝟐= 〖𝜺 _𝑴 𝒃 〗 ^𝟏 𝑪_𝑴+ 〖𝜺 _𝑵 𝒃 〗 ^𝟏 𝑪_𝑵

0.125= 〖 602 𝑏 〗 ^1 𝐶_𝑀+ 〖 92 𝑏 〗 ^1 𝐶_𝑁

0.398= 〖 92 𝑏 〗 ^1 𝐶_𝑀+ 〖 1204 𝑏 〗 ^1 𝐶_𝑁

CM =|■8(0.125&92@0.398&1204)|/|
■8(602&92@92&1204)| 0.000159 1.59*10-4 M
=(0.125∗(1204)−0.398∗(92))/(602∗(12
04)−92∗(92) )=
CN =|■ 8(602&0.125@92&0.398)|/|
■ 8(602&92@92&1204)| 0.000318 3.18*10-4 M
=(602∗(0.398)−92∗(0.125))/(602∗(1204)
−92∗(92) )=
 A400 A460
longNi 0.269 0.134
longCo 0.057 0.091

MUESTRA %T400 %T460

A 25 22
B 48 67
C 13 27
λ= 510 nm
b= 1 cm
P0= 95.5
C= 0.0001 M
P= 15.3
ɛ= ???

%𝑻=𝑷/𝑷_𝟎 ∗𝟏𝟎𝟎

%𝑇= 15.3/95.5∗100 = 16

𝐴=−log⁡(%𝑇)

A=−log⁡(16%) = 0.795

𝐴= ɛ𝑏𝐶
ɛ=𝐴/𝑏𝐶

ɛ= 7953.1194 lt/mol*cm