v k f k'

0 2234 1.000 2234.00 10

1 1985 0.909 2183.50
2 1759 0.833 2110.80
3 1552 0.769 2017.60
4 1362 0.714 1906.80
5 1188 0.667 1782.00
6 1027 0.625 1643.20
7 878 0.588 1492.60
8 740 0.556 1332.00
9 611 0.526 1160.90
10 491 0.500 982.00
11 415 0.476 871.50
12 466 0.455 1025.20
13 568 0.435 1306.40
14 663 0.417 1591.20
15 753 0.400 1882.504000.00
16 838 0.385 2178.80
17 918 0.370 2478.60
18 994 0.357 2783.20
19 1067 0.345 3094.303500.00
20 1135 0.333 3405.00

3000.00

2500.00

2234.00
2183.50
f(x) = 2110.80
37.6341558442x + 1502.8060606061
2017.60
R² = 0.1165938925
2000.00 1906.80
1782.00
1643.20
1492.60
1500.00
1332.00

1160.90

982.00
1000.00 871

500.00

0.00
0 5 10
500.00

0.00
0 5 10
 k
2500

2000

1500

1000

500

0
0 5 k' 10 15 20 25

3405.00

3094.30

2783.20

2478.60

2178.80
02.8060606061

1882.50
0
643.20
1591.20
1492.60

1332.00 1306.40
1160.90
1025.20
982.00
871.50

10 15 20 25
10 15 20 25
25
25
Alícuot o
Concentracion absorbancia PATRON EXTERNO
as r
1 ### 0.0020 0.3436
1.35 ### 0.0027 0.4786
1.7 ### 0.0034 0.5944
2.05 ### 0.0041 0.7031
2.4 ### 0.0048 0.8273
concentracion
0.200 cafeina= 1,46 mg/ml

ESTÁNDARES EXTERNOS
0.9
0.8
0.7
f(x) = 170.2714285714x + 0.0104771429
0.6 R² = 0.9987288055
Absorbancia
0.5
0.4
0.3
0.2
0.1
0
0.0015 0.0020 0.0025 0.0030 0.0035
Concentración (mg/ml)

Alícuot
as
Aforo
estanda Muestra Concentracion absorbancia
r1 ### 1 0.002 0.2572
2 ### 1 0.004 0.2773 0.7000
3 ### 1 0.006 0.3951 0.6000 f(x
R²
4 ### 1 0.008 0.5961
0.5000
5 ### 1.0000 0.010 0.6626
Absorbancia

Concentracion 0.4000
cafeina= 20,6 mg/ml
0.3000
0.200
0.2000

0.1000

0.0000
0.001 0.002

ESTANDAR INTERNO
Alícuot
as absorbancia
Aforo Concentracion paracetamol Concentracion
estanda
r Muestra paracetamol cafeina (mg/ml)
2 ### 2 0.0040 0.2229 0.003
3 ### 2 0.0060 0.3087 0.003
4 ### 2 0.0080 0.5139 0.003
5 ### 2 0.0100 0.6035 0.003
6 ### 2 0.0120 0.7481 0.003

0.200
ON EXTERNO

STÁNDARES EXTERNOS

14285714x + 0.0104771429
88055

5 0.0030 0.0035 0.0040 0.0045 0.0050

Concentración (mg/ml)

ADICION ESTANDAR

ADICIÓN DE ESTÁNDAR
0.7000

0.6000 f(x) = 56.48x + 0.09878

R² = 0.9396576272
0.5000
Absorbancia

0.4000

0.3000

0.2000

0.1000

0.0000
0.001 0.002 0.003 0.004 0.005 0.006 0.007 0.008 0.009 0.010 0.011
Concentración (mg/ml)

absorbancia Concentracion Absorbancia

cafeina paracetamol/ Paracetamol/
cafeina cafeina
0.112 1.333 1.990
0.1087 2.000 2.840
0.113 2.667 4.548 PATRÓN INTERNO
7.000

6.000 f(x) = 1.8031580191x - 0.4945310215

R² = 0.9903757063
5.000
 PATRÓN INTERNO
0.1089 3.333 5.542 7.000
0.1125 4.000 6.650 f(x) = 1.8031580191x - 0.4945310215
6.000
R² = 0.9903757063
Concentracion 5.000
cafeina= 3,395 mg/ml

Absorbancia
4.000

3.000

2.000

1.000

0.000
1.000 1.500 2.000 2.500 3.000 3.50

Concentración (mg/ml)
ATRÓN INTERNO

031580191x - 0.4945310215
03757063
ATRÓN INTERNO

031580191x - 0.4945310215
03757063

2.000 2.500 3.000 3.500 4.000 4.500

Concentración (mg/ml)