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Planar Chromatography

Planar Chromatography... What you need: a method TLC or HPTLC precoated layers suitable equipment know how

Planar Chromatography...
A method... Developed and validated by CAMAG

CAMAG Laboratory

www.camag.com

Download methods for free...

www.camag-laboratory.com

Planar Chromatography... What you need : a method TLC or HPTLC precoated layers suitable equipment know how

Planar Chromatography...
TLC/HPTLC precoated layers available at CAMAG or at your lab supplier

TLC vs. HPTLC


Mean particle size: Particle distribution:

TLC
10 - 15 m wide

HPTLC
5 - 7 m narrow

Layer thickness:
Migration distance:

250 m
100 - 150 mm

100, 200 m
36 - 72 30 - 70 mm

Number of samples: max. 12

Migration time:
Solvent use: Detection limit:

30 - 200 min
50 ml Abs 100 - 1000 ng Fluor 1 - 100 ng

3 - 20 min
5 - 10 ml 10 - 100 ng 0,1 - 10 ng

Comparison TLC-HPTLC
TLC plate 20 x 20 cm
12 cm migration 25 mL solvent 32 min.

HPTLC plate 20 x 10 cm
7 cm migration 10 mL solvent 10 min.

Flavonoids (Ph.Eur.4)

Planar Chromatography... What you need: a method TLC or HPTLC precoated layers suitable equipment know how

Planar Chromatography
Sample application Chromatogram development Post-Chrom. Derivatization Detection & quantitative evaluation Documentation

Planar Chromatography
Sample application Sample application is the most critical step in the TLC procedure. Errors which occur during sample application, cannot be compensated for in chromatogram development or evaluation.

Planar Chromatography...
step by step
Sample Application

Sample Application
Spot versus Band application

Nanomat

Linomat Spray-on technique


volume 1L 2L 5L volume 10L

volume 1L 2L

Sample application - semi automatic LINOMAT5


Sprays on samples as narrow bands
Larger volumes can be applied (1 - 200 L) Precise sampling of programmed volumes

Sample application for:


qualitative quantitative

preparative separation
LINOMAT

Linomat 5

Turret with dosing syringe

Plate holder

Keypad & Display

Sample application: fully automatic ATS4


PC - controlled Application of spots, bands or rectangles Reduced time for application Improved reproducibility GLP compliant

Suitable for danger substances

Automatic TLC Sampler 4


Protection cover
Waste bottle Rinsing bottle

Turret with dosing syringe

Plate holder

Rack for sample vials or micro-titer plates

Display

Automatic TLC Sampler 4


Contact transfer = Spots Spray-on = Bands Spray-on = rectangular

Planar Chromatography... step by step Sample Application Chromatogram Development Evaluation Documentation

Chromatogram development
Manual separation via Glass tank
Automatic isocratic separation via ADC Fully automatic gradient separation via AMD2

ADC2
Chromatogram development with standardized conditions in a conventional glass tank
fully automatic saturation controlled humidity controlled

unsurpassed reproducible
qualifiable IQ/OQ

TLC -sprayer

Planar Chromatography... step by step Sample Application Chromatogram Development Evaluation Documentation

Visual inspection in Viewing box

Densitometry

TLC Scanner 3

Scanning the chromatogram


Integrated track

Densitometry

Spectrum Scan
Display of the selected spectra

Spectrum Library
Analysis spectrum compared with a spectrum from the library

Detection multi wavelength scan


A selection of up to 31 WL is possible

Classical densitometry Multi Wavelength Scan


One track at all wavelengths

Planar Chromatography... step by step Sample Application Chromatogram Development Evaluation Documentation

TLC VISUALIZER: Documentation, Evaluation, Archiving

TLC Visualizer
Design has been enhanced to further improve the homogeneity of the illumination of the plate: 254 nm, short wave UV - Reflectance 366 nm, long wave UV - Reflectance White light - Reflectance and/or Transmission. Ergonomic design: Easy access in to the illumination compartment via large sliding door. separate viewing window with UV-protection Timer for automatic switch-off of the light sources

Documentation system TLC VISUALIZER

Manual Optimization
If desired, a region of interest can be defined, in which the brightest spot is automatically found.

winCATS with TLC Visualizer

RF-Tool to measure RF of the substances

Planar Chromatography...
is GLP/GMP compliant: All significant parameters of a TLC method are recorded and electronically stored.

Planar Chromatography... What you need: a method TLC or HPTLC precoated layers suitable equipment know how

Planar Chromatography...
Know How... Application Lab Training courses individual Training

Planar Chromatography...
Why CAMAG?

> 40 years of experience satisfied customers individual solutions competent After Sales Service training courses

CAMAG worldwide
CAMAG
Muttenz / Switzerland www.camag.ch

CAMAG Berlin / Germany


www.camag-berlin.de

CAMAG Scientific Inc. North Carolina / USA


www.camagusa.com

CAMAG China Supporting Unit Hong Kong & Guangzhou www.camag-china.com


CAMAG Regional Support Unit Bombay / India www.hptlc-anchrom.in

Conclusion ...

..... no laboratory is
complete without a

modern HPTLC system!

Thanks for your attention !