Writing Problem and Hypothesis

Statements for Science Research(7)

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Setting of work proposal :
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Work problem :
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Quantitative specification of problem :

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Importance of problem :
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Project need : ,
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Work objective : ?
Methodology to achieve objective
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Anticipated results :
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Contribution to field :
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()
Widely distributed in aquatic environments, bacteria in the genus
Vibrio are considered autochthonous bacteria in marine and estuarine waters.
Capable of causing infection in humans, some Vibrio species have been
isolated from a variety of intestinal and extraintestinal sites. Specifically, this
bacteria can cause gastroenteritis in humans. PCR method is the conventional
means of detecting the target with the tdh gene or trh gene, in which the
specificity in the chromosome of V. parahaemolyticus gene of chitinase can be
used as the detection marker. However, this method is
inefficient in terms of detecting the target with the tdh gene or trh gene.
(NOTE : Add 2-4 sentences that describe characteristics of the problem or
statistics that reflect its severity) For instance, PCR
method requires seven days to authenticate the results. However, the PCR
method can be used as long as the effect can be authenticated. The molecular
biology method is an effective means of authenticating the results efficiently.
The inability to detect the target with the tdh gene or trh
gene more efficiently will lead to a higher incidence of food poisoning in
humans from aquatic foods, with an especially high incidence during the spring
season. Therefore, a PCR-based molecular biology method
must be developed to identify a particular gene of Vibrio parahaemolyticus
rapidly.
()
A PCR-based molecular biology method can be
developed to identify a particular gene of Vibrio parahaemolyticus
rapidly. To do so, this gene in a chromosome can be
extracted from bacteria. Amplification of this gene can be achieved by
using the PCR method. PCR-based assays can then be made by
preparing all of the genomic DNAs of the strains in Tris-EDTA buffer
(TE; pH 8.0)according to previous literature. Next, the purity and the
amount of DNA in each preparation can be estimated colorimetrically,
with the DNAs subsequently stored at 4C until further use. Additionally,
whether germs can be found in seafood can be determined using the
PCR method. As anticipated, the proposed PCR-based
molecular biology method can rapidly identify the presence of marine
bacteria in the early stages. In addition to providing quick
results with a high degree of sensitivity, the proposed method can yield
rapid and accurate results to detect Vibrio parahaemolyticus in the
environment. (NOTE : Add 2-4 more sentences that describe more
thoroughly how the proposed method contributes to a particular field or
sector)
()
Gene expression requires that many
proteins interact with a regulated element. However,
modulation of gene expression has not been thoroughly
investigated, making it impossible to determine the period
and location of gene expression. However,
expression of the same gene in a diverse cell can be
regulated based on different expressions. (NOTE : Add 2-
4 sentences that describe characteristics of the problem or
statistics that reflect its severity) and,
thus, cannot be expressed 100%.
The inability to thoroughly understand the mechanism of
distinct gene expression makes it impossible to understand
how this unique mechanism affects embryo development.
Therefore, the mechanism of gene
expression in a cell must be elucidated owing to the
importance of acquiring genes in a cell for embryo
development.
()
The mechanism of gene expression in a cell
can be elucidated owing to the importance of acquiring
genes in a cell for embryo development.
To do so, the regulatory role of a gene in the upstream as
promoter and enhancer can be analyzed by adopting
molecular cloning methods. Next, the identified gene can
be linked with a regulating gene to clarify how gene
expression sites are regulated. As anticipated,
analysis results can clarify the mechanism of gene
expression in cells and regulation of tissue-specificity
genes. Results of this study can provide a
valuable reference for efforts to develop a therapeutic
method of gene expression for patients with genetic defects.
(NOTE : Add 2-4 more sentences that describe more
thoroughly how these results contribute to a particular field
or sector)
Further details can be found at
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