Grupo Punto isoeléctrico Grupo

carboxilo
amino
terminal terminal

H2N Ala Tyr Glu Val Ser COOH

9.69 10.07 4.25 2.21
pka 9.69 -------------- 10.07 4.25 -------------- -------------- 2.21
Carga neta
Carga del Ala Tyr Glu Val Ser Carga del péptido
pH amino carboxilo
2.21---------
4.25--------- +1 -------------- 0 0 -------------- -------------- 0 +1
9.69--------- +1 -------------- 0 0 -------------- -------------- -1 0
10.07------- +1 -------------- 0 -1 -------------- -------------- -1 -1
0 -------------- 0 -1 -------------- -------------- -1 -2
0 -------------- -1 -1 -------------- -------------- -1 -3
  𝟐 . 𝟐𝟏 + 𝟒 . 𝟐𝟓
𝒑𝑰 = =𝟑 . 𝟐𝟑
𝟐