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Biologically active filters – An advanced water treatment process for contaminants of

emerging concern

EDGAR DE JESÚS CAMPO CASTRILLÓN

Presentado a
Lesly Tejada

UNIVERSIDAD DE CARTAGENA
FACULTAD EN INGENIERÍA
MAESTRÍA EN INGENIERÍA QUÍMICA
CARTAGENA DE INDIAS, D.T y C.
2017
PAPER

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INTRODUCCIÓN

BAF/Biologyc Actived
al actived
Carbon/GAF
Filters
Negro de Humo

water treatment
process
Analysis in removing
biodegradable
natural organic
matter (NOM)

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Materiales y Métodos
8 colums: 4 with BAF+GAC/ 4
BAF(dual media)

25-44mL/min

10-18 min residence time

Backwash (cleaning)

Ozone generator3 g O3/hr

Source water was collected from


the PVWC Plant after coagulation
179 and clarification and was
maintained at 21-25ºC.

acetaminophen and ibuprofen (analgesics); erythromycin, sulfamethoxazole, and trimethoprim (antibiotics);carbamazepine


(antiepileptic); atenolol (β-blocker); gemfibrozil (blood lipid regulator); TCEP (fire retardant); cotinine (nicotine metabolite);
aminotriazole, atrazine, and DEET (pesticides); caffeine (psychomotor stimulant); 17β-estradiol (steroid); and, iopromide
(x-ray contrast agent).
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Materiales y Métodos
Monitoring
A number of parameters were monitored over the course of this study (14 months).Oxygen consumption and pH change in
the filters were investigated based on dissolved oxygen (DO) and pH measurements.

Growth of bioluminescent bacteria Pseudomonas fluorescens P-17 and Spirillum sp.

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RESULTADOS

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RESULTADOS

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CONCLUSIONES
Existing filters in water treatment plants can be converted into BAFs for the treatment of CECs.

GAC BAFs showed greater CEC removal compared to dual media BAF.

EBCT played a critical role in CEC removals in BAFs.

Pre-ozonation dosage based on the ozone demand showed the most significant improvement in CEC removals.

TCEP and iopromide were the most recalcitrant compounds for BAFs.

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PAPER

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ABSTRACT
La contaminación del agua con antibióticos causada por la descarga de aguas residuales y aguas residuales con antibióticos no
tratados de la cría de animales es cada vez más grave, especialmente en los países en desarrollo.

Los métodos de tratamiento del agua contaminada con antibióticos son complicados, costosos y lentos.

En el presente paper se presenta una membrana de ultrafiltración enzimática capaz de degradar antibióticos de manera rápida
y a bajo costo.

La encima Penicinilasa fue inmovilizada en BPPO (poly-2,6-dimethyl-1,4-phenylene oxide) y mostró un desempeño


excelente, con una capacidad de 335 L/m2 ∗ h con un paso simple. Una porción de 25 cm2 puede proveer agua a una familia
de 4 personas (10 L por día).

Keywords
Antibiotic pollution
Enzyme immobilization
Water treatment
Asymmetric polymer membrane
High stability
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INTRODUCCIÓN

Combination
Antibiotic of enzyme and
pollution membrane
Negro de Humo technology

BPPO
Penicillinase
membrane

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Materiales y Métodos

Membrana BPPO + Degradación BP


• BPPO Inmov. Penicinalasa empleando P-BPPO
• FTIR spectrometer • PBS buffer under
• Penicilinasa
•20%wt BPPO+NMP+DI H2O • 20 scans •DI H2O + 3mg/L room
• Bencilpenicilina sódica •Pieza 3.5 cm +2mL • Análisis morfológico por Bencilpenicilina sódica temperature and
• N-Methyl-2- Penicilinasa y 4 h shaker SEM •Filtrada por membrana P-
60rpm- TA BPPO
re-used for daily
pyrrolidone(NMP) (scanningelectronmicrosc degradation of
•Wash with DI H2O + sln Na •1-5 x 10^5 Pa
opy)
CL(1M) •Recolectadas muestras penicillin
Prueba de estabilidad
Materiales Carac. Membranas
de la membrana

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Materiales y Métodos

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RESULTADOS

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RESULTADOS
Autors measured the protein concentration of the
original penicillinase solution and the reaction solution
after im- mobilization : 0.07 mg/ml and 0.006 mg/ml, more
than 90% of the protein was immobilized into BPPO
membrane.

Based on the concentration difference of enzyme before


and after immobilization, it is estimated that the loading
amount of immobilized enzyme is 6.2 mg/g (protein
weight/BPPO membrane weight)

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RESULTADOS
Characterization of immobilization mechanism

Peaks at 1540cm^-1; 1650cm^-1; 1285cm^-1

The changes of FTIR spectra after enzyme immobilization


confirm the successful covalent bonding of peni- cillinase
onto the BPPO membrane surface rather than simple
physical adsorption.

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RESULTADOS
Performance of P-BPPO membrane for penicillin-polluted water treatment

Peaks at 1540cm^-1; 1650cm^-1; 1285cm^-1

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RESULTADOS
Performance of P-BPPO membrane for penicillin-polluted water treatment

4%, 7% 13% decrease of effect due to


changes in pressurre

Accordingto the degradation rateand flux, at


a pressure of 5x10^5 Pa, the degradation
capacity ofP-BPPO is estimated to be 2170
mg/(m^2 h)
There was no detectable decrease of activity
after 10 times reuse and 10 days storage under
room temperature

The free penicillinase lost 80% of its activity


over7days storage at room temperature

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CONCLUSIONES
The excellent stability of our P-BPPO membrane under room condition suggests its availability for industrial or domestic
application.

This prompted us to conduct a degradation test using tap water containing antibiotic, to eval- uate feasibility of P-BPPO for
household water treatment.

While there was no detectable decrease of penicillin concentration by pristine BPPO, a fully degradation of penicillin was
realized by just a single passing through P-BPPO .

Also:

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REFERENCIAS
R. Gothwal,T.Shashidhar,Antibiotic pollution in the environment:a review, Clean. -Soil.AirWater43(2015)479–489.

H. Park,Y.K.Choung, Degradation of antibiotics (tetracycline,sulfathiazole, ampicillin)


using enzymes of glutathion S transferase,Hum. Ecol.Risk Assess.13(2007)1147–1155.

M.Chhabra,S.Mishra,T.R. Sreekrishnan, Laccase/mediator assisted degradation of


triaryl methane dyes in a continuous membrane reactor,J.Biotechnol.143(2009)69–78.

R.Gnanasekaran, H.A.Mottola,Flow-injection determination of penicillins using


immobilized penicillinase in a single bead string reactor,Anal.Chem.57 (1985)1005–
1009.

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GRACIAS
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