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CHAPTER 10:
INTEGRATED BIOSEPARATION
SCHEME FOR PRODUCT
ISOLATION, PURIFICATION
AND FORMULATIONS UNITS
FOR BIOPROCESS
MISS. RAHIMAH BINTI OTHMAN
(Email: rahimah@unimap.edu.my)
COURSE OUTCOME 1 CO1)
CHAPTER 7 : Upstream Processing In Bioprocess Plant.
CHAPTER 8 : Bioreaction Design In Bioprocess Plant.
CHAPTER 9 : Downstream Processing In Bioprocess Plant.
UPSTREAM PROCESSES
Media Formulation
Inoculum Equipment
and
Preparation Sterilization
Sterilization
BIOREACTOR - FERMENTER
DOWNSTREAM PROCESSES
INTEGRATION
In engineering, system
integration is the bringing
together of the component
subsystems into one system and
ensuring that the subsystems
function together as a system.
In information technology,
systems integration is the
process of linking together
different computing systems and
software applications physically
or functionally.
EXAMPLE: The research
roadmap of Process Integration
study in Yeast & Ethyl Alcohol
(Y&EA) plant.
Physical model of Y&EA plant.
Fig. 5. Scheme of the integrated bioprocess. Limonene-saturated air was
supplied to the bioreactor by leading it through a washing flask filled with
pure limonene (A).Glycerol (B) and limonene (C) were continuously fed via
peristaltic pumps. Using another peristaltic pump (D) the culture broth was
continuously recirculated through a recovery loop containing a column with
a fluidized bed of anion exchanger Amberlite IRA 410 Cl (E).
CHEMICAL MOLECULE STRUCTURE
INTEGRATION
What is “Product Isolation,
Purification, and Formulation Units?
Removal of insolubles is the first step and involves the capture of the
product as a solute in a particulate-free liquid, for example the separation
of cells, cell debris or other particulate matter from fermentation broth
containing an antibiotic.
- Typical operations to achieve this are filtration, centrifugation,
sedimentation, flocculation, electro-precipitation, and gravity settling.
Product Polishing describes the final processing steps which end with
packaging of the product in a form that is stable, easily transportable and
convenient.
- Crystallization, desiccation, lyophilization and spray drying are
typical unit operations.
How to CHOOSE the appropriate sequencing of processes?