Documentos de Académico
Documentos de Profesional
Documentos de Cultura
BIT 612
FINAL PRESENTATION
Outlines
1. Introduction
2. Objectives
3. Methods + Results
4. Conclusion
5. References
Introduction
Azzam et al., 2012
Cryptococcus neoformans
Naturally free living and
found in plants and animals
Can be opportunistic
pathogen, and also causing
fatal meningoencephalitis
Fungi
Exposed Serially
Cultured in spotted Incubated Survived
by diluted
YPD broth on YPD in 30 C, 3 Colony
radiation (100 -
medium plate d counted
for 1 h 104)
(30 C, 24 h)
Laccases play a role in melanin production. While melanin physically shields the cell
and helps quenching ROS (Dadachova & Casadevall, 2008), but it does not play a
role in mitigating other effects such as DNA damage repairing.
Remodeling of transcriptome profiles in response to gamma radiation
of C. neoformans
Microarray
C neoformans
Exposed by 3 kGy gamma RNA
Cultured in YPD qRT-PCR
radiation (0’, 30’, 60’) extraction
medium
Rig1∆ and Rig2∆ mutants showed severe sensitivity to all of the DNA-
damaging stresses that was tested. While, Rig3∆ mutant exhibited increased
susceptibility to MMS and 4-NQO.
Radiation-induced genes, such as RIG1, RIG2, and RIG3, contributed to
gamma radiation resistance, as well as DNA damage stress responses, in C.
neoformans.
Identification and characterization of a novel bZIPTF, Bdr1, for
radiation resistance in C. neoformans
qRT-PCR
Microarray Unknown
data transcript Phylogenetic
CD Analysis
Analysis
0.1
Deletion of BDR1 caused severe growth defects upon genotoxic agents exposure.
Identification of downstream targets and upstream regulators of Bdr1 for radiation
resistance in C. neoformans
Gamma radiation
Downregulation
Ergosterol
Protein translation Metabolic process
synthesis
References
Jung KW, Yang DH, Kim MK, Seo HS, Lim S, Bahn YS. 2016. Unraveling Fungal Radiation Resistance
Regulatory Networks through the Genome-Wide Transcriptome and Genetic Analyses of Cryptococcus
neoformans. mBio. 7(6): e01483-16. **
Azzam EI, Jay-Gerin JP, Pain D. 2012. Ionizing radiation-induced metabolic oxidative stress and
prolonged cell injury. Cancer Letter. 327:48–60.
Dadachova E, Bryan RA, Huang X, Moadel T, Schweitzer AD, Aisen P, Nosanchuk JD, Casadevall A.
2007. Ionizing Radiation Changes the Electronic Properties of Melanin and Enhances the Growth of
Melanized Fungi. PLoS ONE, 2(5), e457.
Dadachova E., & Casadevall A. 2008. Ionizing Radiation: how fungi cope, adapt, and exploit with the help
of melanin. Current Opinion in Microbiology, 11(6), 525–531.