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Amebiasis
(Amebic Dysentery)
Causal agent: Entamoeba histolytica is well recognized
as a pathogenic amoeba.
1- trophozoite
2- precyst
3- cyst(1, 2, 4 nuclei)
Trophozoite chractere
Size: 12-60m in diameter;
Non-invasive form ( minuta) / E. dispare
Invasive form (magna) contain RBC, E. histolytica
Pseudopodia:
Motility:
Ectoplasm:
Endoplasm: may be contain ingested RBC
Nucleoplasm:
invasive form
Non-invasive form
Different form of E.histolytica cyst
Life cycle
Life cycle
Epidemiology
Prevalence of amebic infection varies with level of sanitation
and generally higher in tropics and subtropics than in
tempearate climates.
- pathogen zymodemes
3 cytophagocytosis
Paraclinical Diagnosis:
Sigmoidoscopic examination:
precence of a grossly normal mucosa between the ulcers serves to
differentiate amebic from bacillary dysentery,( the entire mucosa being
involvoed in bacillary dysentery).
Hepatomegally
C.B.C. : leukocytosis in Amebic dys. rises above 12000 per
microliter, but counts may reach 16000 to 20000 per microliter.
Laboratory Diagnosis
Entamoeba histolytica must be differentiated from other intestinal
protozoa including: E. coli, E. hartmanni, E. dispare,
Microscopy
B
A
Each trophozoite has a single nucleus, which has a centrally placed karyosome
and uniformly distributed peripheral chromatin .
Trophozoites of Entamoeba histolytica with ingested
erythrocytes (trichrome stain)
E F
The ingested erythrocytes appear as dark inclusions.
Erythrophagocytosis is the only morphologic characteristic that can be
used to differentiate E. histolytica from the nonpathogenic E. dispar .
Cysts of Entamoeba histolytica
/E. dispar
GHI
H I
Cysts of Entamoeba histolytica/E.
dispar ,permanent preparations stained
with trichrome.
Immunodiagnosis
(Antibody Detection)
1- Antibody detection