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E.C. 3.5.1.5
Hidrolasa
Conversin de urea en NH3 + CO2
Se encuentra en bacterias,
plantas, algas, hongos,
invertebrados y suelos.
Cofactor Ni2+
Primera protena en ser
caracterizada por difraccin de
rayos X.
Introduccin
Propiedades de la ureasa:
Temeperatura ptima: 30C
Tiempo ptimo incubacin: 60 s
Km =36,49 mM
Vmax = 87,72 U/g
pH ptimo: 7,4
Inhibidores: Metales pesados (Hg2+)
Reaccin principal
Mtodo de Berthelot
Mtodo de Nessler
Valoracin cido-base
Reaccin de Berthelot
Precipitacin con
acetona enfriada.
Cromatografa de
intercambio inico.
(DEAE-Celulosa)
Cromatografa por
filtracin en gel
Inmovilizacin
Alginato de sodio en
perlas
Inhibicin enzimtica
Extracto crudo de
ureasa
Inmovilizacin en
alginato
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