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Characteristics
IMMATURE CELLS
MATURE CELLS
Cell is large
Nucleoli are present
Nucleus - round
Round, lobulated or
segmented
High N:C
Low N:C ratio
(nucleus:cytoplasm) ratio
Erythrocyte Maturation
Erythropoiesis red cell production and
maturation
CD34 hematopoietic stem cell marker
Erythropoiesis is regulated by
erythropoietin, which is produced by
the kidneys
Strongest stimulus for erythropoietin
production: tissue hypoxia
Erythrocyte Maturation
Erythrocyte Maturation
Maturation Stages:
Six morphological stages of erythrocyte maturation
Erythrocyte Maturation
Erythrocyte Maturation
General Guidelines:
The ASCP published the following general guidelines
Rubriblast
Pronormoblast
) m,
This is the earliest(erythrocyte,
cell size up to 20
The nuclear:cytoplasmic ratio is 8:1
1-3 nucleoli, nucleus has dark areas of DNA
Chromatin is fine and uniform and stains intensely
Deep blue cytoplasm with no granules
Prorubricyte (Basophilic
normoblast)
RNA
Rubricyte (Polychromatophilic
normoblast)
Metarubricyte (Orthochromic
normoblast)
Mature Erythrocyte
size
It is a biconcave disc and hence referred to as a
discocyte
In a Wright stain, a central pale area is revealed which
fades gradually into the reddish pink cytoplasm
Granulocyte
Maturation
MYELOBLAST
staining chromatin
2-5 nucleoli; dark blue cytoplasm
No cytoplasmic granules
1% of the nucleated cells in the bone marrow
PROMYELOCYTE
chromatin
1-3 nucleoli; dark blue cytoplasm
Cytoplasm has large, nonspecific/primary
granules containing myeloperoxidase
2-5% of the nucleated cells in the bone marrow
MYELOCYTE
be differentiated
12-18 m; NC ratio 2:1; round nucleus with coarse chromatin
Light blue to light pink cytoplasm; prominent golgi apparatus
Specific/secondary granules that contain hydrolytic enzymes
(e.g. alkaline phosphatase and lysozyme) are present
Nonspecifc granules are present and may still stain
Last stage capable of cell division
METAMYELOCYTE
BAND (STAB)
nucleus
Cytoplasm is pink and filled with specific/secondary granules
Primary granules are present but do not stain
Stored in the bone marrow and released when there is increased
demand
Earliest stage that can be seen in the periperal blood of a
healthy person
MATURE GRANULOCYTES
NEUTROPHIL EOSINOPHIL
10-15 m; NC ratio
1:3
12-16 m
BASOPHIL
10-15 m
Nucleus is usually
bilobed
Nucleus is
unsegmented or
bilobed
Cytoplasm contains
fine lilac secondary
granules
Cytoplasm contains
large, bright redorange secondary