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ENZYME(CATALASE): A BIOMARKER
FOR TYPE 2 DIABETES
DEPARTMENT OF BIOTECHNOLOGY
INTERNAL GUIDE
Ch.N.VANI
EXTERNAL GUIDE
DR. KAISER JAMIL
SHAYISTA NAAZ
09J21A2316
INTRODUCTION
DIABETES:
Type I diabetes:
In type 1 diabetes, the pancreas
undergoes an autoimmune attack
by the body itself, and is rendered
incapable of making insulin.
Type II Diabetes:
Insulin resistance
When sugar cannot enter the cells
they start building up in the blood
at high amounts in the blood
stream is called hyperglycemia.
catalase
2H2O2
2H2O + O2
ESTIMATION OF PROTEIN
1 ml of
ESTIMATION OF CATALASE
QUNTIFICATION BY SPECTROPHOTOMETER
The isolated DNA is of pure quality and is not contaminated
specific primers.
Reagents
Volume
Water
18.3l
Buffer
2.5l
dNTPs
1l
Forward Primers
1l (20 pmol/l)
Reverse Primers
1l (20 pmol/l)
Taq Polymerase
0.2l
DNA sample
1l
CONCLUSION
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