Discussion#1:Regulation of Ventilation Rate Experiment B Data:

t=0 t=6 t=12 t=18 average Fish#1 Fish#2 Fish#3 Fish#4 98 117 130 112 57 115 150 108 93 97 153 130 115 124 139 142 90.75 113.25 143 123

Table 1: The ventilation rates and average ventilation rate of all four fish at the time intervals of 0, 6, 12, and 18.

Weight Fish(g) Length (cm)

Fish 1 Fish 2 Fish 3 Fish 4 22 23.5 2.4 22.1 11 11 5.5 10.5

Table 2: The weight of all four fish in grams and the length of all four fish in centimeters.
Fish 1 Fish 2 Fish 3 Fish 4 1.512 1.887 2.383 5 5 33 2.05

Ventilation rate/sec

Table 3: The average ventilation rate/sec of fish 1-4.

Fish 1 Ventilation rate/g body weight 4.125

Fish 2

Fish 3 Fish 4 59.58 4.819 3 5.565

Table 4: The average

ventilation rate/g body weight of fish 1-4.

Figure 1: Average ventilation rate/sec of fish 1-4.

Figure 2: Average ventilation rate/g body weight of fish 1-4.

Experiment C Data:
# of ventilation averag e

50F
t=0 t=6 t=12 t=18 t=24 avera ge

Fish 1 69 65 86 87 88 79

Fish 2 80 82 100 93 100 91

Fish 3 57 75 88 96 102 83.6

84.533 33

Table 5: The ventilation rates and average ventilation rate of all three fish at the time intervals of 0, 6, 12, and 18 in 50 degree water.
Fish 1 18.2 6.4 11.8 9.0 Fish 2 15.1 6.6 8.5 9.0 Fish 3 13.4 6.7 6.7 8.0 avera ge

weight ttl (g) weight tray (g) weight fish (g) length (cm)

9.0 8.7

Table 6: The weight of all three fish in grams and the length of all three fish in centimeters, along with weight of the tray and weight of the tray and fish combined in 50 degree water. The average weight and average length are also shown.

70F
t=0 t=6 t=12 t=18 t=24 avera ge

Fish 1 113 131 120 114 95 114.6

Fish 2 106 136 118 121 105 117.2

Fish 3 120 133 134 133 119 127.8

averag e

119.86 67

Table 7: The ventilation rates and average ventilation rate of all three fish at the time intervals of 0, 6, 12, and 18 in 70 degree water.
Fish 1 14.9 6.4 8.5 8.5 Fish 2 14.8 6.8 8.0 8.5 Fish 3 17.1 6.8 10.3 9.0 avera ge

weight ttl (g) weight tray (g) weight fish (g) length (cm)

8.9 8.7 3

Table 8: The weight of all three fish in grams and the length of all three fish in centimeters, along with weight of the tray and weight of the tray and fish combined in 70 degree water. The average weight and average length are also shown.
Fish 1 135 169 183 175 175 167.4 Fish 2 93 160 160 115 127 131 Fish 3 133 148 153 161 173 153.6 averag e

t=0 t=6 t=12 t=18 t=24 avera ge

150.66 67

Table 9: The ventilation rates and average ventilation rate of all three fish at the time intervals of 0, 6, 12, and 18 in 80 degree water.
Fish 1 17.1 6.2 10.9 9.0 Fish 2 21.7 6.4 15.3 10.5 Fish 3 20.5 6.9 13.6 9.5 avera ge

weight ttl (g) weight tray (g) weight fish (g) length (cm)

13.3 9.7

Table 10: The weight of all three fish in grams and the length of all three fish in centimeters, along with weight of the tray and weight of the tray and fish combined in 80 degree water. The average weight and average length are also shown.
50 1.408888 89 70 1.9977 78 80 2.5111 11

vent rate/sec

Table 11: The average ventilation rate/sec of the fish in 50, 70, and 80 degree water.
vent rate/g body weight 50 9.392592 59 70 13.417 91 80 11.356 78

Table 12: The average ventilation rate/g body weight of the fish in 50, 70, and 80 degree water.

Figure 3: The average ventilation rate/sec of the fish in 50, 70, and 80 degree water.

Figure 4: The average ventilation rate/g body weight of the fish in 50, 70, and 80 degree water. Experiment D Data: Trial 1
t=0 t=6 t=12 t=18 # of ventilation small medium Fish fish 137 124 164 156 163 148 155 139

large fish 130 118 121 119 5

t=24 avera ge

169 157.6

137 140.8

112 120

Table 13: The ventilation and average ventilation rate of the small, medium, and large fish in 70 degree water at time intervals of 0, 6, 12, and 18 for Trial 1.
small Fish weight ttl (g) weight tray (g) weight fish (g) length (cm) 8.4 6.1 2.3 6.0 medium fish 15.2 6.4 8.8 8.5 large fish 29.7 7.0 22.7 11.0

Table 14: The weight of all three fish in grams and the length of all three fish in centimeters, along with weight of the tray and weight of the tray and fish combined in 70 degree water. The average weight and average length are also shown for trial 1. Trial 2
t=0 t=6 t=12 t=18 t=24 avera ge small Fish 141 136 128 170 139 142.8 medium fish 131 130 127 133 120 128.2 large fish 120 117 124 111 100 114.4

Table 15: The ventilation and average ventilation rate of the small, medium, and large fish in 70 degree water at time intervals of 0, 6, 12, and 18 for Trial 2.
Fish 1 weight ttl (g) weight tray (g) weight fish (g) length (cm) 9.8 6.1 3.7 6.5 Fish 2 13.2 6.3 6.9 7.5 Fish 3 31.1 6.4 24.7 11.5

Table 16: The weight of all three fish in grams and the length of all three fish in centimeters, along with weight of the tray and weight of the tray and fish combined in 70 degree water. The average weight and average length are also shown for trial 2.
small Fish medium large 6

fish trial 1 trial 2 vent. Rate/Sec vent. Rate/Sec 2.6 2.4 2.3 2.1

fish 2.0 1.9

Table 17: The average ventilation rate/sec of the small, medium, and large fish in 70 degree water for trials 1 and 2.
small Fish trial 1 trial 2 vent. Rate/g bodyweight vent. Rate/g bodyweight 68.5 38.6 medium fish 16.0 18.6 large fish 5.3 4.6

Table 18: The average ventilation rate/g body weight of the small, medium, and large fish in 70 degree water for trials one and 2.

Figure 5: The average ventilation rate/sec of the small, medium, and large fish in 70 degree water for trials 1 and 2.

Figure 6: The average ventilation rate/gram bodyweight of the small, medium, and large fish in 70 degree water for trials 1 and 2.

Discussion In experiment B, a relationship can be drawn between size and ventilation rate. Table 1 shows fish three to have the highest ventilation rate and table 2 shows fish three to weigh the least out of all the other fish. Tables 3 and 4, along with figures 1 and 2, demonstrate that fish 3 had the highest average ventilation rate per second and the highest average ventilation rate per gram of body weight than compared to fish 1, fish 2, and fish 4. In experiment C, a relationship can be drawn between environmental temperature and ventilation rate, in addition to size. Table 5 demonstrates that the three fish in 50 degree water had the smallest average ventilation rate, while table 9 demonstrates that the three fish in 80 degree water had the largest average ventilation rate. Table 7 shows the three fish in 70 degree water to have an average ventilation
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rate larger than the fish in 50 degree water, but smaller than the fish in 80 degree water. Table 6 and 8 show the weight and length of each fish in 50 and 70 degree water, respectively. There was not that much difference in weight and length. However, table 10 shows the fish in 80 degree water to weight much more and have a greater length than the fish in 70 and 50 degree water. Table 11 and figure 3 show the ventilation rate per second was greatest in the fish in 80 degree water and smallest in the fish in 50 degree water. Table 12 and figure 4 show the ventilation rate per gram of body mass to be largest in the fish in 70 degree water and smallest in the fish in 50 degree water, with the fish in 80 degree water being in the middle. In experiment D, an experiment was conducted involving a small size fish, a medium size fish, and a large fish. The ventilation rates were measured in two trials. Our hypothesis was that the largest fish would have the smallest ventilation rate and that the small fish would have the largest ventilation rate. Tables 13-16 demonstrated that exact phenomenon. Table 17 and figure 5 showed the ventilation rate per second to be greatest in the small fish and least in the large fish for both trials. Table 18 and figure 6 showed the ventilation rate per gram of bodyweight to be greatest in the small fish and least in the large fish. A number of conclusions can be reached regarding these experiments. For experiments B and D, it was clear that the smallest fish had the highest ventilation rate and the largest fish had the smallest ventilation rate. The reason why smaller organisms have a higher ventilation rate is that they rely on oxygen diffusing into the cell. They need to generate body heat quickly because they lose heat so quickly compared to larger fish because in small fish, the skin surface area makes up a high percentage of their body. Small fish do not have the mass for their bodies to act as a heat sink. In experiment C, the three fish in eighty degree water had the highest ventilation rate compared to the fish in 70 and 50 degree water. However, the average ventilation rate/ gram body weight of the three fish in the 70 degree water was greater than the
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fish in 50 or 80 degree water, which conflicts with the surface area to volume ratio theory. A reason for this may have been that there was a discrepancy between the number of breaths counted and the actual number of breaths the fish took in one minute. The ventilation rate per second was highest in the fish in 80 degree water. Experiment C shows the ventilation rate in a warmer climate is higher than in a cooler one.

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