Table of Contents

API 2000 LC/MS/MS TurboIonSpray

Ion Source Manual

Table of Contents
API 2000 TurboIonSpray Ion Source Manual. . . . . . . . . . . . . . . . . . . . . . . . . . 1
About This Manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1
Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1
TurboIonSpray Source Components . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3
Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6
Optimizing the TurboIonSpray . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7
Changing The Peek Tubing Transfer Line . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .12
Changing The Stainless Steel Sprayer Tube . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .14
Changing The Grounded Union/Splitter Fitting . . . . . . . . . . . . . . . . . . . . . . . . . . . . .14
Appendix A - Consumable Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Appendix B - Troubleshooting. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .17

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Table of Contents

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 API 2000 TurboIonSpray Ion Source Manual

API 2000 TurboIonSpray Ion Source

Manual
About This Manual
This manual contains the instructions required to operate the API 2000 TurboIonSpray ion
source.

Conventions
Within this manual, the following conventions are used:

WARNING! Indicates an operation that may cause personal injury if

precautions are not followed.

CAUTION! Indicates an operation that may cause damage to the instrument

if precautions are not followed.

NOTE: Emphasizes significant information in a procedure or description.

Introduction
TurboIonSpray is an Atmospheric Pressure Ion Source in which pre-formed ions in
solution are emitted into the gas phase with or without the application of heat. In this way,
quasi-molecular ions can be generated from very labile and high molecular weight
compounds with no thermal degradation.
TurboIonSpray is the technique by which a liquid sample is pumped by a liquid
chromatograph (LC) pump or syringe drive through a sprayer probe, which is maintained
at a high voltage, and is nebulized into the Ion Source creating a mist of highly charged
droplets. Once inside the Ion Source, the droplets evaporate causing the ions to enter the
gas phase by a low energy process called Ion Evaporation.
The use of an orthogonal heated gas extends the rugged and versatile technique of
TurboIonSpray to accept higher flow rates with improved sensitivity. TurboIonSpray will
accept flows from 5 to 1000 µL/min of solvent compositions from 100% aqueous to 100%
organic, such as acetonitrile, without splitting. This allows the use of 1 mm, 2 mm, and 4.6
mm analytical columns with or without splitting.
A heater probe directs a jet of heated dry gas (up to a maximum of 500°C) at the mist
produced by the sprayer. The gas is sprayed across the orifice at an angle of approximately
45° with respect to the Curtain Plate. The liquid spray emerging from the TurboIonSpray
is directed at an angle of about 45° from the opposite direction (or 135°). The
TurboIonSpray effluent and the heated dry gas intersect at an angle of approximately 90°
near the orifice. This interaction helps focus the TurboIonSpray stream and increases the
rate of droplet evaporation resulting in an increased ion signal.

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API 2000 TurboIonSpray Ion Source Manual

TurboIonSpray Setup
TurboIonSpray is ideally suited for LC/MS/MS quantitative analyses. The sensitivity
increases that are achieved with this technique are both flow rate and analyte dependent.
In the conventional IonSpray source sensitivity decreases with increased flow rate, while
the heated TurboIonSpray process increases ionization efficiency, especially at the higher
flow rates; this results in improved sensitivity. Sensitivity is compound dependent and
compounds of extremely high polarity and low surface activity usually show the greatest
sensitivity increases. The TurboIonSpray technique is mild enough to be used with labile
compounds such as peptides, proteins, and thermally labile pharmaceuticals.

NOTE: The difference between conventional IonSpray and TurboIonSpray is that in the
TurboIonSpray, the ionization of the sample is achieved with additional heater gas
delivered to aid in the desolvation of the spray. Typically the TurboIonSpray is used at
higher flow rates (greater than or equal to 40 µL/min).

Features
The following list outlines the features of the TurboIonSpray ion source:
• Able to function as conventional IonSpray source when the Heater Gas is turned off.
• Able to function with flow rates from 1 µ L/min to 1000 µL/min.
• Able to vaporize 100% aqueous to 100% organic solvents.

Specifications

Temperature Range
• Probe temperature from 0° to 500°C

Liquid Chromatograph
• Interfaces to any liquid chromatograph system

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 API 2000 TurboIonSpray Ion Source Manual

TurboIonSpray Nebulizer and Heater Gases (Gas 1 and 2)

• Zero Grade Air regulated to 100 psi

TurboIonSpray Source Components

Ion Source Housing
The ion source housing forms a seal with the instrument interface assembly to prevent any
solvent or sample from escaping into the laboratory environment. Any liquid or vapour
entering the source is safely transferred to a waste receptacle and fume hood with the aid
of the source exhaust pump.
The source housing incorporates two motion mechanisms (that adjust the position of the
sprayer, described below), two windows to allow the user to view the position and
condition of the sprayer, a Peek tubing transfer line, a grounded union/splitter fitting, two
latches used for locking the source in place and integrated voltage/gas connectors. The
connections for the voltage, heater and gases are made automatically once the source is
locked to the interface.
.

TurboIonSpray Ion Source

Sprayer Probe
The TurboIonSpray source contains a fixed angle adjustable sprayer probe. The sprayer
probe is adjustable in two directions: towards the orifice from a scale of 0 to 12 (adjusted
by the horizontal adjusting ring mounted on the TurboIonSpray probe), and up and down
towards the orifice from 0 to 12 by the Lateral Adjustment control on the top of the Ion
Source.

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API 2000 TurboIonSpray Ion Source Manual

Sprayer Probe

Sprayer Probe and Adjustments

The TurboIonSpray probe gas, or Nebulizer gas (Gas 1) and voltage (IS) are set at the
applications computer using the software. The connections for the voltage and gas are
made automatically once the source is locked to the interface.

Transfer Line and Grounded Union/Splitter

A short piece of Peek tubing (30 cm, 0.0025” ID) connects the sprayer probe inlet to a
grounded fitting that can be used either as a union or splitter. Since the liquid being
sprayed from the source is in contact with high voltages, the transfer line must be
connected to the grounded fitting.

Peek Tubing

Grounded Union/Splitter

PEEK Tubing and Grounded Union/Splitter

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 API 2000 TurboIonSpray Ion Source Manual

WARNING! The use of the Peek tubing transfer line connected to the
grounded fitting is mandatory since it prevents any exposed peripherals
connected to the source from floating at high voltage.

WARNING! Peek tubing (or fused silica) with Peek fittings must be used as
the transfer line. Metal tubing or fittings must not be used. The use of metal
may result in the exposure of high voltage to the user.

Refer to the procedure for changing the grounded fitting from a union connection to a
splitter described later in this document.

Heater Probe
The heater probe temperature is set in the applications computer software by modifying
the TEM parameter. The correlation between the parameter value of TEM and the actual
heater temperature is direct (for example TEM 350 is equal to 350°C at the heater). The
heater gas is also set in the applications computer software. The connections for the heater
and gas are made automatically once the source is locked to the interface.
The heater probe temperature is maintained by the Temperature Control Board (TCB)
mounted behind the IonSource panel (below the IonSource housing). The TCB adjusts the
flow of power to the heater element as a function of the difference between the actual
heater temperature and the temperature setting in the applications computer. The probe
temperature is monitored by a thermocouple connected directly to the heater element
which maintains the temperature within ±5 degrees of the applications computer setting.
The operating range for the probe is from approximately 100° to 500°C.

NOTE: The temperature is controlled by monitoring the output of a thermocouple

connected to the heater surrounding the metal tube. At the temperature control board the
thermocouple output is compared with the temperature setting, the difference determines
the power flow to the heater.

The operating temperature should be adjusted relative to LC mobile phase composition

and flow rate. In general, a combination of heat and gas flow that just allows the liquid
spray to reach dryness before reaching the curtain plate will provide optimum
performance.
Heater gas flows of approximately 6 L/min can be considered optimum for conditions
where heat is required. Ionization efficiency is improved with the input of heat for all
liquid flow rates. However, at lower flow rates (<20 µL/min) the gains are small. Heat
inputs may be desirable under low flow conditions for the additional reason of enhancing
in-source (orifice-skimmer) fragmentation. Temperatures as low as 80°-100°C have
proven useful for enhancing this capability (phosphopeptide mapping for instance) when
operating at low flow rates.

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API 2000 TurboIonSpray Ion Source Manual

Exhaust Pump
The TurboIonSpray Source requires that the Source Exhaust system is properly connected
and functioning (refer to the figure Exhaust Supply Connection Points under Source
Exhaust Pump in the Optimizing the TurboIonSpray section). A filtered nitrogen or air gas
supply (free from pump oil) is delivered to the Source Exhaust pump at 60 psi pressure at
a flow of at least 4 L per minute. The Source Exhaust pump is used to vent solvent vapors
which develop in the Ion Source plenum.
It is recommended that these vapors be passed through a trap vented to a fume hood or
outside port.

WARNING! Failure to provide proper ventilation of the ion source can result
in hazardous vapors being released into the laboratory environment.

Installation
The TurboIonSpray source connects to the Vacuum Interface housing. Two latches
mounted on the source housing secure the TurboIonSpray against the vacuum interface.

To install the TurboIonSpray on the API instrument:

1. Ensure that the two source latches are in the unlocked (up) position.
2. Align the two guide pins on the source with the vacuum interface receptacles.

Guide Pins

Guide Pin Alignment

3. Slide the source along the guide pins towards the interface as far as it can go without
using excessive force.
4. Turn the two source latches to the locked (down) position.
5. The TurboIonSpray Nebulizer and Heater Gas (Gas 1 and 2), the sprayer high voltage
and heater connectors are automatically engaged once the source is locked in position.

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 API 2000 TurboIonSpray Ion Source Manual

6. Connect the liquid sample tubing to the grounded fitting which is connected to the
inlet end of the sprayer probe. Ensure that all fittings are properly seated in order to
minimize dead volumes.

NOTE: In order to reduce the band broadening of sample with solvent, thus maximizing
sensitivity, the use of 0.005” ID Peek, or fused silica tubing between the TurboIonSpray
source and the injector is recommended.

To remove the TurboIonSpray from the API instrument:

WARNING! Ion source may be hot for several minutes after it is removed
from the instrument.

1. Stop all scans and place the instrument in Standby or Overnight Quit status.
2. Turn off the liquid flow entering the ion source.
3. Disconnect the liquid sample tubing from the grounded fitting.
4. Turn the two source latches to the unlocked (up) position.
The sprayer high voltage and heater connectors are automatically disengaged once the
source is unlocked.
5. Slide the source along the guide pins away from the interface to remove it.

Optimizing the TurboIonSpray

The following section outlines the practical considerations which must be made when
optimizing the TurboIonSpray performance. It is intended to provide the qualitative
information necessary to aid the operator in quantifying the separate operating parameters.
TurboIonSpray performance depends on the following factors:
• Sprayer position
• TurboIonSpray (IS) voltage
• Nebulizer gas (NEB)
• Curtain gas (CUR)
• Heater Gas flow and Temperature (Gas 2 and TEM)
• Declustering Potential (DP) and Focusing Potential (FP) voltage (compound
dependent)
• Solvent Composition
Optimum performance on standard compounds is relatively easy to achieve and little
adjustment or tuning is required once the Source is optimized.
With experience, the operator will develop a personal optimization method which works
best. In general, once the sprayer is set up and optimum factors have been determined,
little or no readjustment of the values is required on a day-to-day basis.
For tuning purposes a compound with a known molecular ion should be introduced either
by continuous infusion or flow injection. Infusion is preferred because it provides a
continuous flow of sample. The tuning compound should have characteristics similar to
the sample to be analyzed during normal operation and should be introduced at the same
liquid flow rate.

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API 2000 TurboIonSpray Ion Source Manual

CAUTION! When optimizing the Sprayer position make certain not to spray
directly down the orifice. Spraying down the orifice may contaminate the
Vacuum Interface and Vacuum Chamber ion optics and could impact
instrument performance.

CAUTION! To avoid possible damage to the instrument, always view the

sprayer tip through the side port when adjusting its position.

CAUTION! If unattended user operation is intended through the use of

sample control, Ensure that LC shut-off is in use to prevent flooding of the
plenum chamber.

TurboIonSpray Probe Position

The position of the TurboIonSpray Probe relative to the Orifice and to the Heater Probe is
an important factor in optimizing the TurboIonSpray performance. The probe should point
between 5 and 10 mm off axis with respect to the center of the orifice. The distance of the
Heater Probe from the orifice plane is fixed, but the TurboIonSpray can be adjusted using
the scale on the side of the Sample inlet arm. Changing from low solvent flow rates
(40µL/min) to high solvent flow rates (1mL/min) requires that the TurboIonSpray be
repositioned further away from the orifice to prevent solvent penetration through the
orifice into the mass spectrometer.

OPTIMAL CURTAIN PLATE

TURBOIONSPRAY
TARGET

ORIFICE
40 µL/min
200 µL/min
1mL/min

TurboIonSpray Positioning Across the Orifice

Also as the aqueous composition of the carrier solvent increases at high flow rates (1 mL/
min), the more visible the spray becomes and the further away from the orifice it should be
directed. See the figure above where the areas indicated in the diagram for the different
flow rates are the optimum target areas for the TurboIonSpray liquid spray. The circle
immediately around the orifice (for example the part of the orifice plate which is visible
when viewing the front of the interface) should remain clear of solvent or solvent drops at
all times.

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 API 2000 TurboIonSpray Ion Source Manual

The best position is usually a few millimeters off axis to the bottom of the curtain plate
aperture. Multiply charged proteins and peptides introduced at a few microliters per
minute usually require the sprayer to be as close as possible to the Curtain Plate.

TurboIonSpray Voltage (IS)

In positive mode, singly charged compounds usually require a high probe voltage of
between 4000 to 5500 V. In negative mode, compounds usually require a lower voltage,
between 3000 to -4500 V.

NOTE: If the TurboIonSpray voltage is set too high a blue glow can be seen at the tip of
the TurboIonSpray indicating a corona discharge. This will result in decreased sensitivity
and stability of the ion signal.

Nebulizer Gas (Gas 1)

Optimize for signal stability and sensitivity. Typically a value of 10 to 45 psi is used as
applied by the applications computer.

Curtain Gas Flow (CUR)

The Curtain Gas ensures a stable clean environment for the sample ions entering the mass
spectrometer. The gas curtain prevents air or solvent from entering the analyzer region of
the instrument while permitting the sample ions to be drawn into the vacuum chamber by
the electrical fields generated between the Vacuum Interface and the TurboIonSpray
needle. The presence of the solvent vapor or moisture in the analyzer region of the mass
spectrometer contaminates the Q0 Rod Set causing a reduction in resolution, stability,
sensitivity, and an increase in chemical background noise. As a general rule, the Curtain
Gas flow should be set as high as possible without reducing the signal significantly (for
example start at a lower value and increase the flow until the signal starts to decrease).
In order to prevent instrument contamination the Curtain Gas flow should be optimized
at the highest possible setting, but never below 11 psi, that does not result in a
significant reduction in signal intensity.
It is suggested that the TurboIonSpray be operated with curtain gas settings adjusted to the
highest flow rate possible (never below 11) without signal loss.

Heater Gas (Gas 2)

The Heater Gas (Gas 2) aids in the evaporation of solvent which aids in increasing the
ionization of the sample. The higher the liquid flow or the higher the aqueous composition
of the solvent, the higher the Heater Gas temperature and gas flow required. However, too
high a temperature can cause premature vaporization of the solvent, and result in a high
chemical background noise, while too high a Heater Gas flow can produce a noisy, or
unstable signal.

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API 2000 TurboIonSpray Ion Source Manual

The table below provides recommended operating conditions for the TurboIonSpray at
three different flow rates. For each flow rate, the Curtain Gas flow rate (from setting 11 to
45 at the applications computer) should be as high as possible. The solvent composition
used for optimization was 1/1 Water/Acetonitrile. These conditions represent a starting
point from which to optimize TurboIonSpray. By an iterative process, the various settings
can be optimized using Flow Injection Analysis to obtain maximum signal-to-noise for the
compound of interest.

TurboIonSpray Suggested Operating Settings Table

Flow Rate TurboProbe Heater Gas Nebulizer

µL/min Temp. (°C) (GAS2) Flow Setting Position
(L/min) PSI (GAS 1)
V H
40 µL/min. 300 6 65 20 2 0
(200 to 500) (4 to 8) (40-90) (10-30)
200 µL/min. 400 7 75 30 3 5
(300 to 500) (4 to 8) (40-90) (15-35)
1 mL/min. 475 8 90 50 6 10
(450 to 500) (4 to 8) (40-90) (20-55)
V = Vertical position
H = Horizontal position

Turbo Temperature (TEM)

The quantity and type of sample affect the optimal TurboIonSpray temperature. At higher
flow rates the optimal temperature increases. A more significant factor is the composition
of the solvent. As the organic content of the solvent increases the optimal probe
temperature should decrease. With solvents consisting of 100 percent methanol or
acetonitrile the probe performance may optimize as low as 300°C. Aqueous solvents
consisting of 100 per cent water at flows approximately 1mL/min require a minimum
probe temperature of 425°C. Normal optimization is usually performed in increments of
25°C.
The TurboIonSpray is normally used with sample flow rates of 40 µL/min to 1000 µL/
min. The heat is used to increase the rate of evaporation and this improves ionization
efficiency resulting in increased sensitivity. See the table above for recommended
temperatures.

CAUTION! Do not operate the TurboIonSpray with probe temperatures

greater than 500oC.

Declustering Potential (DP) and Focusing Potential (FP) Voltages

Optimal Declustering Potential and Focusing Potential operating conditions with the
TurboIonSpray source should be set high enough to reduce the chemical noise but low
enough to avoid fragmentation. Start with the Focusing Potential (FP) at 300V and the
Declustering Potential (DP) at 20V.

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 API 2000 TurboIonSpray Ion Source Manual

NOTE: The fragmentation energy of a compound is a function of its structure and

molecular weight. Generally lower molecular weight compounds require less energy -
lower Declustering Potential and Focusing Potential voltages to induce fragmentation. For
some labile compounds, DP values of between 5 to 10 may be required with the
TurboIonSpray.

In general terms, the higher the Declustering Potential and Focusing Potential voltages,
the greater the energy imparted to the ions entering the analyzing region of the mass
spectrometer. The energy helps to decluster the ions and to reduce the chemical noise in
the spectrum resulting in an increase in sensitivity. Increasing the voltages beyond optimal
conditions can induce fragmentation before the ions enter the mass filters resulting in a
decrease in sensitivity. In some instances this fragmentation can prove a valuable tool
providing additional structural information.

Solvent Composition
Commonly used solvents and modifiers are acetonitrile, methanol, propanol, water, acetic
acid, formic acid, ammonium formate and ammonium acetate. The modifiers such as
TEA, sodium phosphate, TFA and dodecyl sodium sulfate are not commonly used because
they complicate the spectrum with their ion mixtures and cluster combinations. They may
also suppress the strength of the target compound ion signal. The standard concentration
of ammonium formate or ammonium acetate is from 2 to 10 millimole per liter for positive
ions and 2 to 50 millimole per liter for negative ions. The concentration of the organic
acids is 0.01% to 0.5% by volume.

Source Exhaust Pump

The Source Exhaust system is required for TurboIonSpray operation. The exhaust pump
draws the solvent vapors from the enclosed source chamber and delivers them to a trap at
the rear of the instrument chassis where they can be collected. The Source Exhaust system
is interlocked to the system electronics, such that if the source exhaust pump is not
operating to specification the instrument electronics are disabled.
The exhaust system lowers the pressure in the source slightly below atmospheric. If the
pressure in the source rises beyond a preset trip point, the instrument High Voltage Power
Supply is disabled.

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API 2000 TurboIonSpray Ion Source Manual

e Curtain Gas Supply Exhaust Supply Valve Waste Out

Gas1/Gas2 Exhaust Waste Out

Exhaust Supply Connection Points

Operating Tips
1. When running the TurboIonSpray, with or without the Heater Probe on, run the
Curtain Gas at as high a flow rate as possible. The Curtain Gas should optimize about
30% higher when TurboIonSpray is in use. This will help to:
a) prevent penetration of the Curtain Gas which can produce a noisy signal,
b) prevent contamination of the orifice, and
c) increase the overall signal to noise ratio
2. The liquid spray from the TurboIonSpray should be directed away from the orifice in
order to:
a) prevent contamination of the orifice,
b) prevent piercing of the Curtain Gas which can create an unstable signal, and
c) prevent electrical shorting due to the presence of the liquid.
3. The higher the liquid flow or the higher the aqueous composition of the solvent, the
higher the temperature and Heater Gas (Gas 2) flow required. However, too high a
temperature can cause thermal degradation of the compound, and result in a high
chemical background noise, while too high a heater gas flow can produce a noisy, or
unstable signal.

Changing The Peek Tubing Transfer Line

A Peek tubing transfer line is used to carry the sample and solvent from the Sample Inlet
to the metal sprayer tube where it is nebulized and ionized. The standard TurboIonSpray
set-up uses a 30 cm, 0.0025” (65 µ m) ID Peek tubing transfer line.

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 API 2000 TurboIonSpray Ion Source Manual

WARNING! The use of the Peek tubing transfer line connected to the
grounded fitting is mandatory since it prevents any exposed peripherals
connected to the source from floating at high voltage.

To exchange the Peek tubing transfer lines in the TurboIonSpray in the

event of blockage of the existing line:
1. Remove the TurboIonSpray and set on its side.
2. Unscrew the Peek tubing fittings from the probe inlet and grounded union/splitter
fitting and discard the old tubing (the fittings may be re-used if they have not been
over-tightened and damaged).

Peek Tubing
3. Obtain a new piece of 0.0025” Peek tubing (it must be at least 30 cm long) and place
Peek connection fittings on each end.

WARNING! Peek tubing (or fused silica) with Peek fittings must be used as
the transfer line. Metal tubing or fittings must not be used. The use of metal
may expose the user to high voltage.

4. Connect the Peek tubing to the probe inlet and grounded union/splitter fitting.

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API 2000 TurboIonSpray Ion Source Manual

Changing The Stainless Steel Sprayer Tube

The standard TurboIonSpray set-up uses a 0.004” (100 µm) ID metal sprayer tube. The
procedure outlined below should be used to exchange the metal tube in the TurboIonSpray
in the event of blockage or degradation of the sprayer tip.
1. Remove the TurboIonSpray and set on its side.
2. Disconnect the Peek transfer line from the probe inlet.
3. Unscrew and remove the black inlet probe fitting. A Peek union, metal spring and the
metal sprayer tube can now be removed from the probe.
4. Unscrew the fitting holding the metal tube to the Peek union. Remove the tube from
the fitting and discard. Do not discard the fittings.

Metal Sprayer tube removed

5. Place a new sprayer tube and ferrule in the fitting and screw into the Peek union.
Ensure that the sprayer tube is placed as far as it can go into the Peek union to
guarantee a leak-proof seal (two wrenches should also be used to tighten the fittings).
6. Place the metal spring and metal tube/Peek union back into the probe.
7. Place the black inlet probe fitting over the Peek union and tighten. This fitting is used
to adjust the protrusion of the metal sprayer tube tip at the end of the probe. The metal
tube should protrude approximately 0.75 mm, although a protrusion between 0.5 to
1.25 mm is also acceptable.

Changing The Grounded Union/Splitter Fitting

A Peek tubing transfer line connects the sprayer probe inlet to a grounded fitting that can
be used either as a union or splitter. Since the liquid being sprayed from the source is in
contact with high voltages, the transfer line must be connected to the grounded fitting.

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 API 2000 TurboIonSpray Ion Source Manual

WARNING! The use of the Peek tubing transfer line connected to the
grounded fitting is mandatory since it prevents any exposed peripherals
connected to the source from floating at high voltage.

To change between the union and splitter insert fittings:

1. Remove the TurboIonSpray and set on its side.
2. Disconnect the Peek transfer line from the ground fitting.
3. Disconnect any other fittings or tubing that may be connected to the grounded fitting.
4. The insert within the grounded fitting should now move freely and can be removed by
tilting the source and allowing it to fall out.
5. Place the new insert into the fitting and align it such that all the openings match.
6. Connect the Peek transfer line to the grounded fitting and any other fittings or tubing
that may be used.

WARNING! Peek tubing (or fused silica) with Peek fittings must be used as
the transfer line. Metal tubing or fittings must not be used. The use of metal
may result in the exposure of high voltage to the user.

15
Appendix A - Consumable Parts

Appendix A - Consumable Parts

The following is a list of parts included in the Kit of consumable parts (part number
022559) supplied with the TurboIonSpray assembly.

Item Part No. Description (Quantity)

1 019479 Probe Spring (1)
2 016485 0.0025” ID Peek Tubing (60 cm)
3 019663 Probe Inlet Nut (1)
4 013297 Viton O-Ring, 7/64” ID (2)
5 018783 Viton O-Ring, 4 mm ID (2)
6 018782 Metal Electrode Tube (2)
7 016325 Peek LC Fitting (2)
8 018722 Peek Union (1)
9 019675 Splitter Tee Insert, 0.25 mm bore (1)
10 019674 Peek Ferrule (4)
11 018784 Viton O-Ring, 2 mm ID (2)

16
 Appendix B - Troubleshooting

Appendix B - Troubleshooting
Introduction
The troubleshooting procedures given here are suitable for use by operators who have
access to an ohm meter and are familiar with its use. These procedures do not require
removal of the instrument covers and do not expose the operator to hazardous voltages.
The heater control circuits are protected by a resetable over current device. This device
may be tripped by excessive heater current or by failure to approach the desired operating
temperature within about 5 minutes. When tripped the device will remove power from the
heater. To reset the protective device turn power off the system for about one minute or
remove the ionizer from the system for about one minute.
Diagnosis of the nature of the fault is done by means of ohm meter readings taken across
the pins of the ionizer 24 pin connector on the ionizer which is shown below.

Ionizer 24 Pin Connector

17
Appendix B - Troubleshooting
FAILURE MODES HEATED IONIZER Table
# CAUSE
1 Short circuit in heater.
2 Open circuit in heater.
3 Heater grounded.
4 RTD short circuit.
5 RTD open circuit.
6 RTD grounded.
7 Poor thermal contact
between the RTD and
the heater.
18
SYMPTOMS DIAGNOSIS
No heat. Heater resistance
measured across pins 7 and
19 is less than 15 ohms.
No heat. Heater resistance
measured across pins 7 and
19 is greater than 25 ohms.
No heat. Heater resistance from pin
9 to ionizer frame is less
than 100K ohms.
No heat. RTD resistance measured
across pins 9 and 10 is less
than 100 ohms.
No heat. RTD resistance measured
across pins 9 and 10 is
greater than 300 ohms.
No heat. RTD resistance from pin
10 to ionizer frame is less
than 100K ohms.
When starting the heater The heater works normally
with the temperature set when the temperature is set
to greater than 300°C, to 150°C
the heat comes on for
about 5 minutes and
then goes off.
CURE
Call service to replace
heater.
Call service to replace
heater.
Call service to replace
heater.
Call service to replace
RTD.
Call service to replace
RTD.
Call service to replace
RTD.
Call service to correct
clips attaching the RTD
to the heater.