Concentración de enzima: 2.

0 mM
10.0 mg/mL
Concentración de Ibuprofeno:
48.5 mM

Velocidad de
Velocidad de formación de
[Ácido formación de PGG2 con 10 Porcentaje
araquidónico] de Vmax sin
PGG2 mg/mL
ibuprofeno inhibidor

(mM) (mM/min) (mM/min)

0.50 23.50 16.67 45.5%
1.00 32.20 25.25 62.4%
1.50 36.90 30.49 71.5%
2.50 41.80 37.04 81.0%
3.50 44.00 38.91 85.2%

0.0700

0.0600
f(x) = 0.020276153223497 x + 0.019367646513041
R² = 0.999313894777231
0.0500

0.0400 f(x) = 0.011604400474074 x + 0.019370661845876 sin inhi

R² = 0.999933969087073
Linear (
con inh
0.0300
Linear (

0.0200

0.0100

0.0000
0.00 0.50 1.00 1.50 2.00 2.50

INHIBIDOR COMPETITIVO
 PM ibuprofeno 206.29 mg/mmol

Porcentaje de
Porcentaje de V con Sin Con I50
Vmax con inhibicion inhibidor inhibidor
inhibidor respecto a sin
inhibicion
1/[AA] 1/Vo 1/Vo mg/ml
32.3% 70.9% 2.00 0.0426 0.0600 24.6
48.9% 78.4% 1.00 0.0311 0.0396 35.7
59.1% 82.6% 0.67 0.0271 0.0328 46.9
71.7% 88.6% 0.40 0.0239 0.0270 69.2
75.4% 88.4% 0.29 0.0227 0.0257 91.6

Sin inhibidor: m
646513041 b
Km
Vmax
661845876 sin inhibidor
Linear (sin inhibidor) kcat
con inhibidor
Linear (con inhibidor)

Con inhibidor: m
b
alpha

2.00 2.50 [I]

Ki

ITIVO
0.0116
0.0194
0.5991 mM
51.62 mM/min

25.81 min-1
0.43 s-1

0.0203
0.0194
1.75

10 mg/mL
13.38 mg/mL
64.87 mM