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ABSTRACT 4
3
2
MicroRNA-offset-RNAs (moRNAs) were recently detected as 1
highly abundant class of small RNAs in a basal chordate. Using short
2 s = 0.3L
read sequencing data we show here that moRNAs are also produced 10 s = 0.1L
s = 0.03L
Reads
half of the miRNA families that were present in early Bilateria, while
300
evidence for moRNAs was found only for a relative small fraction of
Reads
100
0
1 INTRODUCTION 121475650 121475700
genomic position
121475750
© The Author (2009). Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org 1
D. Langenberger, C. Bermudez-Santana, J. Hertel, S. Hoffmann, P. Khaitovich, P.F. Stadler
2
Human MicroRNA-Offset RNAs
5 0.60
9 10
4
0.45 microRNAs with moRNAs (N=39)
8 10
density
expressed microRNA families (N=199)
# reads
3
7 10 0.30 all microRNA families (N=277)
2
# moRNA reads
6 10 0.15
1
10
5 1.00
4 0.75
80
3
cdf
60 0.50
# loci
2 40 0.25
1 20
0.00
Metazoa
Eumetazoa
Bilateria
Deuterostomia
Chordata
Olfactores
Vertebrata
Gnathostomata
Teleostomi
Tetrapoda
Amniota
Mammalia
Theria
Eutheria
Epitheria
Euarchontoglires
Primates
Haplorrhini
Hominoidea
0 0
5’moR
5’miR
3’miR
3’moR
1 10 100 1000 10000
# miRNA reads
3
D. Langenberger, C. Bermudez-Santana, J. Hertel, S. Hoffmann, P. Khaitovich, P.F. Stadler
under the auspices of SPP-1174 “Deep Metazoan Phylogeny” to Landgraf, P., Rusu, M., Sheridan, R., Sewer, A., Iovino, N., Aravin,
PFS, a PhD scholarship of the DAAD-AleCol program to CBS, a A., Pfeffer, S., Rice, A., Kamphorst, A. O., Landthaler, M., Lin,
formel.1 grant of the Medical Faculty of the University of Leipzig, C., Socci, N. D., Hermida, L., Fulci, V., Chiaretti, S., Foa, R.,
and the Freistaat Sachsen under the auspices of the LIFE project. Schliwka, J., Fuchs, U., Novosel, A., Muller, R. U., Schermer,
B., Bissels, U., Inman, J., Phan, Q., Chien, M., Weir, D., Choksi,
R., De Vita, G., Frezzetti, D., Trompeter, H. I., Hornung, V.,
Teng, G., Hartmann, G., Palkovits, M., Di Lauro, R., Wernet,
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