proteina (μM) zinc 50 μM (μL) Zimc (μM) RFU

25 100 1 2.123 Chart Title

25 300 3 6.002 30
25 500 5 10.121 f(x) = 1.27875753228121 x + 3.98896508847442
25 750 7.5 14.932 25
25 1000 10 20.321
25 1250 12.5 25.514 20
25 1500 15 24.142
25 1750 17.5 24.731
15
25 2000 20 25.021
Formula ocupada
10
50𝜇𝑀/𝐿 1𝐿/1000000𝜇𝐿 1/0.005𝐿 𝑧𝑖𝑛𝑐 50 𝜇𝑀
(𝜇𝐿)
5

ejemplo con tubo No 1

0
50𝜇𝑀/𝐿 1𝐿/1000000𝜇𝐿 1/0.005𝐿 100𝜇𝑀 0 5 10 15
(𝜇𝐿)=1μM
Chart Title

508847442

15 20 25