RT-PCR primer pairs

Name Forward Reverse

E-Cadherin TGGAGGAATTCTTGCTTTGC CGTACATGTCAGCCAGCTTC
CRUMBS3 CACTGTTTTGCCTTCATCCA CGTCTCCTTGGAGTCCTGAG
HUGL2 TTGAGCGCTTCTCTCTCTCC GACGCTCAGCCACTCACTCT
PATJ AGATGGCCGTTACTCCCTTT ACTGACAGAGGAGCCTGGAA
JAM1 TGCCTAGCCAAGGATGAGAT TGAATGCAGACAAGGTGTTTTC
GJ3B GGCTAGCCTCAGCAGACCT CACCAGCCTGAGCACAGTT
Mucin TACCGATCGTAGCCCCTATG CCACATGAGCTTCCACACAC
P-cadherin AAATGCTCAACCCTGTGTCC ATAGCAACGCAACAGGGAAA
Sciellin CAAGCAGCCTTTGGAAAATC GGCTCTTTTTATGTGTGTGACC
Eva-1 GATGCCTCCATCCTTCTCTG TTCCGGTAATGCTGGAAGAG
Desmocollin2 AAAACTTAGTGTGAGTTTGTTCATCA AAAATGATGAAAATGGACAAAGC

C
Occludin AAGGGAAGAGCAGGAAGGTC CTCCAACCATCTTCTTGATGTG
R-Cadherin TCAGCTCCCTGAACTCATCC TGGGTGCTCATGCTAGACAG
Plakophilin3 CTCGGAACGCTAGGAACAAG AAGTCCTCCTTCCGATAGCC
Epiplakin GATCCAGGAGAAGCTGCTGT AAATAGGAGCCCCGTCTCAG
Periplakin CTGGAGAGGGAACTGGATGA CCTTGTTGACATAGCGGTCA
Actin ATCTGGCACCACACCTTCTAC CAGCCAGGTCCAGACGCAGG

B. ChIP primer pairs

Name Forward Reverse

CRUMBS3 (-261/-8) ATACATACCCCGTGCGTCTC CGTGACCCGGAAAGTTTAGT
CRUMBS3 (-903/-625) CCGGCCATCTGTTTTCTGTA GAAAATTAGCCAGATGTGATGG
CRUMBS3(-2263/-2016) TCGTGAGGGCATATTTAGTTCA GCAGGGGCTCACATACACT
PATJ (-335/-127) TTGATCTTGGGTCTCGCTCT AAGGAGGGAGGACGGTTG

C. Snail-specific siRNAs

ID Forward Reverse
107915 5`-GGUGUGACUAACUAUGCAAtt 5`-UUGCAUAGUUAGUCACACCtt
115742 5`-CCUGUUUCCCGGGCAAUUUtt 5’-AAAUUGCCCGGGAAACAGGtt
115741 5’-CGAGGUGUGACUAACUAUGtt 5`-CAUAGUUAGUCACACCUCGtt.