10

Metal MW (g/mol) Stock mg a pesar (10mL)

CuCl2x2H2O 170.48 1.50E-03 2.5572
Co(CH3COOH)2 249.08 1.50E-03 3.7362
Hg(NO3)2 324.6 1.50E-03 4.869
CaSO4x7H2O 281.1 1.50E-03 4.2165
HgCl2 271.5 1.50E-03 4.0725
FeCl3x6H2O 270.3 1.50E-03 4.0545
Muestra uM
DMPTzTris 1473.9
CuCl2x2H2O 1349.1

eq TzBp eq CuCl2x2H2O V total

1 0 2500
1 0.2 2500
1 0.4 2500
1 0.6 2500
1 0.8 2500
1 1 2500
1 2 2500
1 3 2500
1 4 2500
1 5 2500
1 6 2500
1 7 2500
1 8 2500
1 9 2500
1 10 2500
1 15 2500
1 20 2500
1 25 2500
1 30 2500
1 35 2500
1 40 2500
1 50 2500
1 60 2500
1 70 2500
 20
mg a pesar (20mL)
5.1144
7.4724
9.738
8.433
8.145
8.109

10uL de 3330uM de TzBp = 0,5absorbancia

v constante 20

[TzBp] uM umoles TzBp umoles CuCl2x2H2O [CuCl2x2H2O] uM

11.7912 0.029478 0 0
11.7912 0.029478 0.0058956 2.35824
11.7912 0.029478 0.0117912 4.71648
11.7912 0.029478 0.0176868 7.07472
11.7912 0.029478 0.0235824 9.43296
11.7912 0.029478 0.029478 11.7912
11.7912 0.029478 0.058956 23.5824
11.7912 0.029478 0.088434 35.3736
11.7912 0.029478 0.117912 47.1648
11.7912 0.029478 0.14739 58.956
11.7912 0.029478 0.176868 70.7472
11.7912 0.029478 0.206346 82.5384
11.7912 0.029478 0.235824 94.3296
11.7912 0.029478 0.265302 106.1208
11.7912 0.029478 0.29478 117.912
11.7912 0.029478 0.44217 176.868
11.7912 0.029478 0.58956 235.824
11.7912 0.029478 0.73695 294.78
11.7912 0.029478 0.88434 353.736
11.7912 0.029478 1.03173 412.692
11.7912 0.029478 1.17912 471.648
11.7912 0.029478 1.4739 589.56
11.7912 0.029478 1.76868 707.472
11.7912 0.029478 2.06346 825.384
M de TzBp = 0,5absorbancia
uL= 0.5

V CuCl2x2H2O uL Abs (294) TzBp Abs (273) Complejo

0.000
4.370
8.740
13.110
17.480
21.850
43.700
65.550
87.400
109.251
131.101
152.951
174.801
196.651
218.501
327.752
437.002
546.253
655.504
764.754
874.005
1092.506
1311.007
1529.509
8783.749
Abs (266) Complejo
V TzBp VCuCl2x2H2O VDMSO V H20 V total
10 0 35 2455 2500
10 4.3700244608 35 2450.62997554 2500
10 8.7400489215 35 2446.25995108 2500
10 13.1100733823 35 2441.88992662 2500
10 17.480097843 35 2437.51990216 2500
10 21.8501223038 35 2433.1498777 2500
10 43.7002446075 35 2411.29975539 2500
10 65.5503669113 35 2389.44963309 2500
10 87.400489215 35 2367.59951078 2500
10 109.2506115188 35 2345.74938848 2500
10 131.1007338225 35 2323.89926618 2500
10 152.9508561263 35 2302.04914387 2500
10 174.8009784301 35 2280.19902157 2500
10 196.6511007338 35 2258.34889927 2500
10 218.5012230376 35 2236.49877696 2500
10 327.7518345564 35 2127.24816544 2500
10 437.0024460752 35 2017.99755392 2500
10 546.253057594 35 1908.74694241 2500
10 655.5036691127 35 1799.49633089 2500
10 764.7542806315 35 1690.24571937 2500
10 874.0048921503 35 1580.99510785 2500
10 1092.5061151879 35 1362.49388481 2500
10 1311.0073382255 35 1143.99266177 2500
10 1529.5085612631 35 925.491438737 2500