Absorbancia(NM) [] G/L

0.002 0
0.325 0.5
0.434 1
1.244 2 Curva de calibracion para
2.316 3
7
2.714 5
6
2.925 6
5
f(x) = 1.8148793257x - 0.0823140119
4 R² = 0.9363913092

[] G/L
3 []
Lin
2
1
0
0 0.5 1 1.5 2 2.5 3 3.5

Absorbancia a 540nm
calibracion para DNS

7x - 0.0823140119
[] Vs Absorbancia
Linear ([] Vs Absorbancia)

2.5 3 3.5

ncia a 540nm
 Tiempo Vs Absorbancia
30 40 50 60 70
0 0.968 1.328 1.122 1.531 2.323
15 2.182 1.841 32.02 1.522 52.53
30 1.018 2.364 1.203 1.591 73.11
45 1.144 1.649 1.32 38.16 64.02

[Almidon]
30 40 50 60 70
0 198.325477 197.672113 198.0459822 197.3036881 195.866287
15 196.122188 196.741069 141.969202 197.3200222 104.745603
30 198.234732 195.791876 197.8989753 197.1947941 67.394961
45 198.006054 197.08953 197.686632 130.825716 83.892402

T=30°C T=40°C
1/[] 1/V 1/[] 1/V
0.00509886 0.00546745 0.0050828228 0.00544772
0.00504452 0.0056594 0.0051074642 0.00573941
0.00505035 0.00611244 0.0050738362 0.00614482

T=30°C
0.0062
0.006
0.0058 f(x) = - 7.3184164677x + 0.0428111286
R² = 0.4345870075
0.0056 T=30°C
1/V

Linear (T=30°C )
0.0054
0.0052
0.005
0.00504 0.00506 0.00508 0.0051 0.00512
1/[]

a b Vmax=1/b Km=a*Vmax
-7.3184 0.0428 23.3644859813 -170.990654206

T=60°C
0.012
0.01
f(x) = 1.8673338843x - 0.0039045319
0.008 R² = 0.9978388935
 T=60°C
0.012
0.01
f(x) = 1.8673338843x - 0.0039045319
0.008 R² = 0.9978388935

0.006 T=60°C
1/V
Linear (T=60°C )
0.004
0.002
0
0.0045 0.005 0.0055 0.006 0.0065 0.007 0.0075 0.008
1/[]

a b Vmax=1/b Km=a*Vmax
1.8673 -0.0039 -256.41025641 -478.794871795
 [Azucares Reductores]
30 40 50 60 70
0 1.6745232 2.3278872 1.9540178 2.6963119 4.1337127
15 3.8778118 3.2589309 58.030798 2.6799778 95.254397
30 1.7652682 4.2081236 2.1010247 2.8052059 132.605039
45 1.9939456 2.9104701 2.313368 69.174284 116.107598

Velocidades
30 40 50 60 70
V1 182.90049 183.562928 128.76613652 184.1664429933 91.6878505
V2 176.697326 174.233841 178.16666857 175.6174600267 48.9034409
V3 163.600838 162.738599 163.28887699 96.4436094644 52.3947362

T=50°C T=60°C T=70°C

1/[] 1/V 1/[] 1/V 1/[] 1/V
0.00506791 0.00776602 0.0050679094 0.0054298708 0.00954694 0.01090657
0.00507113 0.00561272 0.0050711278 0.0056941946 0.0148379 0.02044846
0.00764376 0.00612412 0.0076437571 0.0103687534 0.01192003 0.01908589

T=40°C
0.009
0.0064 0.008
0.0062 0.007
0.006 0.006
0.0058 f(x) = - 6.9881701868x + 0.0413334171 T=40°C 0.005
R² = 0.1207794414
1/V

1/V
0.0056 Linear (T=40°C ) 0.004
0.0054 0.003
0.0052 0.002
0.005 0.001
0.00507 0.00508 0.00509 0.0051 0.00511 0
1/[] 0.0045 0.005

a b Vmax=1/b Km=a*Vmax
-6.9882 0.0413 24.213075061 -169.205811138

T=70°C
0.025

0.02 f(x) = 1.7530167038x - 0.0044007123

R² = 0.8103516453
 T=70°C
0.025

0.02 f(x) = 1.7530167038x - 0.0044007123

R² = 0.8103516453
0.015
T=70°C
1/V

0.01 Linear (T=70°C )

0.005

0
0.009 0.01 0.011 0.012 0.013 0.014 0.015 0.016
1/[]

a b Vmax=1/b Km=a*Vmax
1.753 0.0044 227.27272727 398.4090909091

1/T Vs LnVmax
6

1/T LnVmax 5 f(x) = - 131.4963606x + 7.1788893535

0.033 3.15121718 R² = 0.8285359955
4
0.025 3.18689278 1/T LnVmax
LnVmax

3
0.02 4.85363155 Linear (1/T LnVmax)
0.014 5.42615074 2
1
0
0.01 0.015 0.02 0.025 0.03 0.035
1/T
 T=50°C
0.009
0.008
0.007
0.006 f(x) = - 0.2203650054x + 0.0078071868
R² = 0.0847501288
0.005 T=50°C
1/V

0.004 Linear (T=50°C )

0.003
0.002
0.001
0
0.0045 0.005 0.0055 0.006 0.0065 0.007 0.0075 0.008
1[]

a b Vmax=1/b Km=a*Vmax
-0.2204 0.0078 128.205128 -28.2564102564
1/V =
Km/Vmax∗S+1/Vmax
y = ax+b