DEPARTMENT OF MEDICAL LABORATORY SCIENCE

COLLEGE OF HEALTH SCIENCES

HUMAN HISTOLOGY LABORATORY

Activity Manual

FITZGERALD P. CASTILLO,RMT
FITZ GERALD P. CASTILLO, RMT
Instructor

Presented by:

______________________________________
Student

2017

HUMAN HISTOLOGY LABORATORY 1|Page

 PREFACE

This manual is designed for both clinical laboratory scientists and medical laboratory
students who are studying and interested in learning the important aspects of Human Histology.

The authors has been tasked to undertake teachings on laboratory aspects of the subject and
that no laboratory manual is yet available and designed for local use. It has commenced that most
materials that should be presented to the students must be simple and easily absorbed.

The manual is presented in a concise manner that special structures are mentioned for
the student to understand well the theoretical concepts of immunology and serology in applying
it to actual learning method.

Constructive criticism and suggestions from knowledgeable colleagues and students are
encouraged, pointing out items that need to be changed, added or deleted for the improvement
of this manual.

FITZGERALD P. CASTILLO,RMT
FITZ GERALD P. CASTILLO, RMT

HUMAN HISTOLOGY LABORATORY 2|Page

 TABLE OF CONTENTS

Activity
Title Score
Number
1 MICROSCOPY
2 INTERPRETATION OF SECTION
3 THE CELL
4 CELL DIVISION
5 EPITHELIUM
6 CONNECTIVE TISSUE
7 CARTILAGE
8 BONE AND OSSIFICATION
9 BLOOD AND HEMATOPOIESIS
10 MUSCULAR TISSUE

FITZGERALD P. CASTILLO,RMT
Total Score: _______________

HUMAN HISTOLOGY LABORATORY 3|Page

 POLICIES, GUIDELINES AND EQUIPMENTS

A. Requisition of Materials, Reagents and Equipments

1. Filled-out properly the requisition slips of materials and it should be submitted 2-3 days
before the scheduled performance of the laboratory activity to the College Laboratory
Technician.
2. Renew requisition slips for postponed activity or experiments.
3. Second requisition and loses are equally charged to the members of the group or by the
whole class. Breakages incurred during the performance of laboratory experiments or
activities maybe. Performance of experiments or laboratory activities starts from the time
the students get materials up to the time that they return such to the stock room or to the
College Laboratory Technician.
4. Request only the amount of chemicals, reagents, supplies and materials just enough for
the experiment or activity to be performed. Employ microscale or semi-microscale
techniqies whenever possible.
5. The student or the instructor will provide some of the materials if it is not available to
the college stock room that is readily available in the commercial markets.

B. Laboratory Activities and Experiments

1. Each group should be composed of 5-10 members depending on the size of the class.

FITZGERALD P. CASTILLO,RMT
Individual performance is required for small classes.
2. The laboratory instructor should employ strategies to ensure that all students are engaged
during laboratory activities or experiments.
3. The laboratory instructor should conduct pre- and post-laboratory discussion including the
discussion of safety precautions needed in the activity or in the experiment and the
discussion of how wastes should be disposed of properly.
4. The laboratory instructor should discuss other safety measures not included in the safety
policies and important laboratory procedures, operations and techniques specific to
the laboratory subject.
5. Materials borrowed should be returned fifteen (15) minutes before the end of the
laboratory period.
6. Set-up of materials made by any instructor or student should be dismantled by the same
and should dispose of properly the wastes generated from such set-up.

C. Laboratory Safety Policy

1. NO eating, drinking and chewing of gums in the laboratory. Kepp water or beverage
container in your bags. None should be seen on the top of the tables and cabinets.
2. All chemicals in the laboratories should be treated dangerous. Never taste any laboratory
material. Smell materials only if instructed to do so. Smell chemicals by carefully fanning the
top of the tube or bottle so that a little of the vapor is directed towards your nose.
3. Know the proper use and location of the safety materials and equipments (fire
extinguisher, sandbox, medicine cabinet, emergency shower, spill cabinet, fire blanker and
eye wash area).
4. Avoid unnecessary noise in the laboratory
5. Students always stay in their group.

HUMAN HISTOLOGY LABORATORY 4|Page

6. Horseplay and other acts of mischief are strictly forbidden.
7. Never leave an ongoing experiment unattended.
8. Work under the fume hood when using fuming and toxic chemicals.
9. NO student is allowed to enter the stock room unless the instructor told you to do so.
10. Only instructors, stockroom personnel and students in the scheduled laboratory and student
with permission are allowed in the laboratories. Casual visitors are NOT allowed in the
laboratories.
11. Report all breakages and loses to the instructor and to the laboratory assistants.
12. Read or check labels on chemical containers.
13. Label all containers.
14. Secure permission from the laboratory instructor whenever experiments have to
be performed outside of the regular class periods.
15. Do NOT perform any experiment alone or without the supervision of the instructor.
16. Report any chemical spill to your instructor. Spill cabinets have been provided and
the materials should be used ONLY for chemical spills.
17. Always know the hazards and the physical and chemical properties of the materials
used. Material Safety Data Sheet (MSDS) are located in the chemistry stockroom and are
available by asking the laboratory assistants.
18. Report to your instructor any accident, MINOR or MAJOR.
19. Follow all written and verbal directions carefully. If you do not understand the directions,
ask your instructor.
20. Return equipment or apparatuses borrowed from the stock room. Arrange tools after use.
21. Use the necessary Personal Protective Equipment (PPE – laboratory gown, hair net, hand

FITZGERALD P. CASTILLO,RMT
gloves, eye goggles, face mask, etc.).
22. Do NOT wear contact lenses, dangling jewelry and loose clothing. Tie back long hair.
23. Observe good housekeeping practices. Keep working are organized, clean and dry
always.
24. NO using of high-heeled and open shoes in the laboratory.
25. Do NOT apply cosmetics while in the laboratory.
26. Do not use your electronic gadgets inside the laboratory unless the instructor told you to do
so.
27. Put your electronic gadgets into a silent mode to prevent destructions inside the class.
28. Only materials necessary for the activity or experiments should be in the working area.
Books, notebooks and pieces of paper must be kept away from the working area.
29. Throw your wastes in the proper trash bins. All wastes that are not biologically or
chemically contaminated should be surrendered to the laboratory assistants, who
shall place such in the receptacle found in the stock room.
30. Wash hands with soap and water after an experiments.
31. Treat preserved specimens with respect and should be disposed of properly following the
instruction given by your instructor.
32. Turn off faucets, gas tanks and outlets when not in use.
33. If you are the last to use the laboratory , check the following:
a. Faucets, gas tanks and outlets are shut off;
b. Electric fans and lights are put off;
c. Chairs or stools are arranged, and;
d. Windows and doors are closed.

HUMAN HISTOLOGY LABORATORY 5|Page

SPECIAL INSTRUCTIONS:
 
 Use the 1.5”(left) and 0.5”(right, top, bottom) margin with a RED color.
 
 There should be only 4 drawings in each page (divide the working area into 4 squares)
 
 Scale your drawings and label them on the lower-right of the drawing.
 
 In writing, used BLUE ballpoint pen ONLY.
 
 ALWAYS CITE YOUR REFERENCE WITH THE APA format in all of your activities.


Group Leaders will collect the activity papers and arrange it alphabetically then
attached the ¼
sheet of paper(write the members and your group Name) with a
PAPER CLAMP.

I agree and abide by the set policies, guidelines and additional rules provided in print and
discussed verbally our instructor and should carry out all tasks with utmost caution. Should there

FITZGERALD P. CASTILLO,RMT
be instances

Name of the Student (PRINT) _____________________________________

Signature of the Student _____________________________________

Date Signed _____________________________________

HUMAN HISTOLOGY LABORATORY 6|Page

 NAME (SURNAME, FIRST NAME, M.I.) DATE PERFORMED (MM/DD/YYYY) SCORE

COURSE/YEAR/SECTION DATE SUBMITTED (MM/DD/YYYY)

Activity No. 1
MICROSCOPY
Objectives:
1. To manipulate and use properly the microscope.
2. To know the different parts and functions of the microscope.
3. To know the proper care and handling of the microscope.
Materials:

Microscope and Prepared Microscope Slides.
Illustrations:
1. Draw and label the actual binocular microscope.
Research Questions:
1. Define the Following:
a. Parfocal Point
b. Working Distance
c. Resolving Power
d. Refractive Index
e. Resolution
f. Numerical Aperture

FITZ GERALD P. CASTILLO,RMT

2. What are the different parts and functions of a microscope?
3. Enumerate the different Types of Compound Microscope.
4. What is the proper handling and storage of the microscope?

HUMAN HISTOLOGY LABORATORY 7|Page

 NAME (SURNAME, FIRST NAME, M.I.) DATE PERFORMED (MM/DD/YYYY) SCORE

COURSE/YEAR/SECTION DATE SUBMITTED (MM/DD/YYYY)

Activity No. 2
INTERPRETATION OF SECTION
Objectives:
1. To know the different types of cuts or planes in cutting a tissue.
Materials:

Knife, Chopping Board, Calamansi Fruit, Tomato, Hard-Boiled Egg.
Illustrations:
1. Draw and Label the different cuts or sections in each Specimen as mentioned above.
Research Questions:
1. Define the different planes of section being done.
a. Longitudinal
b. Cross section or transverse
c. Tangential
d. Oblique
2. Why are there sections made on tissues or organs?
3. What is the significance of interpreting sections correctly in the study of tissue?

FITZGERALD P. CASTILLO,RMT

HUMAN HISTOLOGY LABORATORY 8|Page

 NAME (SURNAME, FIRST NAME, M.I.) DATE PERFORMED (MM/DD/YYYY) SCORE

COURSE/YEAR/SECTION DATE SUBMITTED (MM/DD/YYYY)

Activity No. 3
THE CELL
Objectives:
1. To be familiarize with the parts and difference between a plant and an animal cell.
Illustrations:
1. Draw and label the plant and animal cell.
Research Questions:
1. Differentiate Plant cell and animal Cell.
2. Identify the different parts of the cell and its normal function in the body.

FITZ GERALD P. CASTILLO,RMT

HUMAN HISTOLOGY LABORATORY 9|Page

 NAME (SURNAME, FIRST NAME, M.I.) DATE PERFORMED (MM/DD/YYYY) SCORE

COURSE/YEAR/SECTION DATE SUBMITTED (MM/DD/YYYY)

Activity No. 4
CELL DIVISION
Objectives:
1. To familiarize and differentiate mitosis and meiosis.
Materials:

Microscope, Prepared Onion Root Microscope Slides and colored atlas.
Illustrations:
1. Draw and label the stages of mitosis and meiosis.
Research Questions:
1. Enumerate and define the different stages of mitosis and meiosis.
2. Differentiate mitosis and meiosis.

FITZGERALD P. CASTILLO,RMT

HUMAN HISTOLOGY LABORATORY 10 | P a g e

 NAME (SURNAME, FIRST NAME, M.I.) DATE PERFORMED (MM/DD/YYYY) SCORE

COURSE/YEAR/SECTION DATE SUBMITTED (MM/DD/YYYY)

Activity No. 5
EPITHELIUM
Materials:
  Microscope and Prepared Microscope Slides in Histology


Illustrations:
1. Draw and label the different types of

epithelium. Format:

FITZ GERALD P. CASTILLO,RMT

Type of Epithelium: _______________
Function: _______________________
Example: _______________________

Research Questions:
1. Define the different types of Epithelium.

HUMAN HISTOLOGY LABORATORY 11 | P a g e

 NAME (SURNAME, FIRST NAME, M.I.) DATE PERFORMED (MM/DD/YYYY) SCORE

COURSE/YEAR/SECTION DATE SUBMITTED (MM/DD/YYYY)

Activity No. 6
CONNECTIVE TISSUE
Specimens:
  Esophagus, Vagina and Urinary Bladder


Illustrations:
1. Draw and label the specimens mentioned

above. Format:

FITZ GERALD P. CASTILLO,RMT

__________________

Research Questions:
1. What is the lining epithelium of the organs observed?
2. Which type of fibers present?
3. Name the types of cells present in this variety of connective tissue?

HUMAN HISTOLOGY LABORATORY 12 | P a g e

 NAME (SURNAME, FIRST NAME, M.I.) DATE PERFORMED (MM/DD/YYYY) SCORE

COURSE/YEAR/SECTION DATE SUBMITTED (MM/DD/YYYY)

Activity No. 7
CARTILAGE
Specimens:
  Trachea, Larynx


Illustrations:
1. Draw and label the specimens mentioned

above. Format:

FITZ GERALD P. CASTILLO,RMT

_______________________________

Research Questions:
1. What is the lining epithelium of trachea and larynx?
2. What type of connective tissue constitutes the organ?

HUMAN HISTOLOGY LABORATORY 13 | P a g e

 NAME (SURNAME, FIRST NAME, M.I.) DATE PERFORMED (MM/DD/YYYY) SCORE

COURSE/YEAR/SECTION DATE SUBMITTED (MM/DD/YYYY)

Activity No. 8
BONE AND OSSIFICATION
Specimens:
  Long Bone(Compact)


Illustrations:
1. Draw and label the specimens mentioned above. (Follow the Format in Activity 7)
2. Draw and label a human bone structure.

1. Define and Differentiate Osteocyte, Osteoblast and Osteoclast in tabular form.

2. Enumerate the different functions of the bone.
3. What are the principal processes involved in bone formation?
4. What is the probable origin of the cells involved in fracture repair?

FITZ GERALD P. CASTILLO,RMT

HUMAN HISTOLOGY LABORATORY 14 | P a g e

 NAME (SURNAME, FIRST NAME, M.I.) DATE PERFORMED (MM/DD/YYYY) SCORE

COURSE/YEAR/SECTION DATE SUBMITTED (MM/DD/YYYY)

Activity No. 9
BLOOD AND HEMATOPOEISIS
Specimens:
  Stained Peripheral Blood Smear


Directions:
  Focus the specimen under Oil Immersion lens. Examine the different types of blood cells seen.


Illustrations:
1. Draw and label the different cell found on the peripheral blood smear.
a. Erythrocytes
b. Leukocytes
i. Neutrophil
ii. Lymphocytes
iii. Monocytes
iv. Eosinophil
v. Basophil
c. Thrombocyte
d. Hematopoiesis
Research Questions:

FITZGERALD P. CASTILLO,RMT
1. What is the function of Blood?
2. What are the different components of Blood?
3. What is hematopoiesis?

HUMAN HISTOLOGY LABORATORY 15 | P a g e

 NAME (SURNAME, FIRST NAME, M.I.) DATE PERFORMED (MM/DD/YYYY) SCORE

COURSE/YEAR/SECTION DATE SUBMITTED (MM/DD/YYYY)

Activity No. 10
MUSCULAR TISSUE
Specimens:
  Small Intestine, Urinary Bladder and Uterus(l.s.)


Illustrations:
1. Draw and label the specimens mentioned above.
Research Questions:
1. What is lumen?
2. Compare the appearance and manner of arrangement of the smooth muscle cells in the organs
of the small intestine from those of the urinary bladder or the uterus.

FITZ GERALD P. CASTILLO,RMT

HUMAN HISTOLOGY LABORATORY 16 | P a g e